CN111747541A - Water treatment microbial agent - Google Patents
Water treatment microbial agent Download PDFInfo
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- CN111747541A CN111747541A CN202010787583.2A CN202010787583A CN111747541A CN 111747541 A CN111747541 A CN 111747541A CN 202010787583 A CN202010787583 A CN 202010787583A CN 111747541 A CN111747541 A CN 111747541A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
Abstract
The invention comprises the following components in parts by weight: 15 parts of microbial agent, 20 parts of microcrystalline cellulose, 15 parts of zeolite powder and 45 parts of activated carbon particles. The microbial agent comprises the following strains in parts by weight: 25 parts of bacillus licheniformis, 25 parts of nitrobacteria and 2 parts of rhodopseudomonas palustris. Different types of microbial bacteria in the strain are matched and promoted with each other, so that the wastewater treatment effect is obviously improved, ammonia nitrogen and nitrite can be degraded, and the ammonia nitrogen and the nitrite can be removed by converting denitrifying bacteria into nitrogen; can convert nitrogen in water into amino acid, accelerate the decomposition speed of organic matters in the water body, participate in the metabolism of sulfur-containing compounds, thereby removing harmful substances such as ammonia nitrogen, phosphorus, sulfide and the like in the water and improving the water quality. The microbial agent has the advantages of short reaction time, large microbial load, strong impact resistance, excellent solid-liquid separation effect, less sludge generation, convenient operation and the like, can permanently and effectively remove organic pollutants in wastewater, has better biological activity and can grow and reproduce when treating wastewater, and realizes the recycling of microorganisms.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a water treatment microbial agent.
Background
The problem of water pollution is getting worse, and domestic sewage enters a municipal sewage plant after being collected by a municipal drainage pipe network and is discharged into a natural water body after being treated to reach the standard. Ammonia nitrogen in the water body can be converted into free ammonia through ionization balance, so that aquatic organisms and human bodies are damaged. The aquatic organisms metabolize ammonia nitrogen and consume a large amount of oxygen, and the method is also an important reason for water deterioration. The ammonia nitrogen can be converted into nitrite nitrogen and nitrate nitrogen in water. Therefore, ammonia nitrogen also becomes the water pollutant index of the key control in China.
Biological denitrification is one of the more economical and effective methods for solving the nitrogen pollution.
Disclosure of Invention
The invention aims to provide a water treatment microbial agent which has the advantages of short reaction time, large microbial load, strong impact resistance, excellent solid-liquid separation effect, less sludge generation, convenience in operation and the like, can permanently and effectively remove organic pollutants in wastewater, has good biological activity and can grow and reproduce when the wastewater is treated, and the recycling of microorganisms is realized.
The technical scheme of the invention is as follows: a water treatment microbial agent comprises the following components in parts by weight: 15 parts of microbial agent, 20 parts of microcrystalline cellulose, 15 parts of zeolite powder and 45 parts of activated carbon particles. The microbial agent comprises the following strains in parts by weight: 25 parts of bacillus licheniformis, 25 parts of nitrobacteria and 2 parts of rhodopseudomonas palustris. Different types of microbial bacteria in the strain are matched and promoted with each other, so that the wastewater treatment effect is obviously improved, ammonia nitrogen and nitrite can be degraded, and the ammonia nitrogen and the nitrite can be removed by converting denitrifying bacteria into nitrogen; can convert nitrogen in water into amino acid, accelerate the decomposition speed of organic matters in the water body, participate in the metabolism of sulfur-containing compounds, thereby removing harmful substances such as ammonia nitrogen, phosphorus, sulfide and the like in the water and improving the water quality.
A preparation method of a water treatment microbial agent specifically comprises the following steps:
step 1: respectively inoculating Bacillus licheniformis, nitrifying bacteria and Rhodopseudomonas palustris to solid culture medium, culturing at 22-32 deg.C for 45-48 hr to obtain strain, scattering the colony, and mixing;
step 2: compounding the thalli according to the formula amount, inoculating the thalli into a liquid culture solution according to the inoculation amount of 1.8-2.4%, culturing for 50-70h at the temperature of 25-35 ℃ and the pH of 7-8 to logarithmic growth phase, centrifuging, concentrating and washing the thalli to obtain a microbial agent;
and step 3: taking microbial agent, microcrystalline cellulose and zeolite powder active carbon particles according to a formula, sterilizing the microcrystalline cellulose and zeolite powder active carbon particles, uniformly mixing with the microbial agent, then adding sterile water, carrying out oscillation culture for 40-60min under an aseptic environment with the rotating speed of 90-130r/min and the temperature of 25-35 ℃, adding sodium alginate and polyvinyl alcohol, and carrying out magnetic stirring uniformly under the condition of 100 plus 150r/min to obtain a mixed solution for later use, wherein the sodium alginate is 0.6 time of the weight of the microbial agent, the weight of the polyvinyl alcohol is 5 times of the weight of the microbial agent, the sodium alginate and the polyvinyl alcohol are used as wall materials for forming capsules, so that microorganisms embedded in the membranes and external microorganisms under an open condition can carry out certain proliferation and maintain normal physiological and biochemical activities by using a carbon source released by the membranes, and have a certain promotion effect on the removal of nitrogen in a water body, the microorganisms in the microcapsule proliferate after being released into the sewage environment;
and 4, step 4: dripping the mixed solution into a mixed solution of sterile calcium chloride and sterile boric acid at a constant speed within 30-40min, crosslinking and curing for 15-25h at the temperature of 30-35 ℃ to obtain gel spheres, quickly washing the gel spheres for 2-3 times by using sterile water, transferring the gel spheres into a chitosan-sodium citrate solution, stirring for 25-30min to obtain microcapsules with the diameters of 1.5-3mM, adding the microcapsules into a 0.07mol/L sodium citrate solution for washing, freeze-drying at the temperature of-4-0 ℃ until the water content is less than or equal to 8 percent to obtain the microbial agent for wastewater treatment, wherein the microbial agent is of a double-layer microcapsule structure, the concentrations of the sterile calcium chloride solution and the sterile boric acid solution are 6 percent, the volume ratio of the sterile calcium chloride solution to the sterile boric acid solution is 1:0.53, preferably, the chitosan-sodium citrate solution is a mixed group of 0.07M sodium citrate solution, 16mg/mL chitosan solution and 0.03mM benalaxyl, the volume ratio of the R (-) -benalaxyl and the S (+) -benalaxyl in the benalaxyl is 1:1:0.005,
Detailed Description
Example 1:
a water treatment microbial agent comprises the following components in parts by weight: 15 parts of microbial agent, 20 parts of microcrystalline cellulose, 15 parts of zeolite powder and 45 parts of activated carbon particles. The microbial agent comprises the following strains in parts by weight: 25 parts of bacillus licheniformis, 25 parts of nitrobacteria and 2 parts of rhodopseudomonas palustris.
A preparation method of a water treatment microbial agent specifically comprises the following steps:
step 1: respectively inoculating Bacillus licheniformis, nitrifying bacteria and Rhodopseudomonas palustris to solid culture medium, culturing at 22-32 deg.C for 45-48 hr to obtain strain, scattering the colony, and mixing. The preparation method of the solid culture medium comprises the following steps: taking 15-25 parts of potato, 1.5-2.5 parts of glucose, 0.2-0.4 part of monopotassium phosphate, 0.1-0.2 part of magnesium sulfate, 1.7-2.2 parts of agar, 10.001-0.003 part of vitamin B and 150 parts of water 130-containing materials by weight, putting the potato into boiling water for boiling for 35-45min, then filtering by using multilayer wet gauze, adding the glucose, the monopotassium phosphate, the magnesium sulfate and the agar into the filtrate, stirring until the mixture is dissolved, and sterilizing to obtain a culture medium for later use;
step 2: compounding the thalli according to the formula amount, inoculating the thalli into a liquid culture solution according to the inoculation amount of 1.8-2.4%, culturing for 50-70h at the temperature of 25-35 ℃ and the pH value of 7-8 to logarithmic growth phase, centrifuging, concentrating and washing the thalli to obtain the microbial agent. The preparation method of the liquid culture medium comprises the following steps: taking 25-30 parts of wheat bran, 15-21 parts of soybean meal, 10-15 parts of peanut shell powder, 0.4-0.7 part of salt, 0.2-0.4 part of sodium dihydrogen phosphate, 0.12-0.16 part of ammonium molybdate tetrahydrate, 0.5-1.3 part of magnesium sulfate, 0.3-0.5 part of ferric sulfate, 0.8-1.5 parts of calcium chloride, 0.2-0.3 part of growth promoter, 0.04 part of tween-800.02, 0.001-0.003 part of vitamin and 120 parts of water 100, adding water according to the weight ratio of 1:5-8, uniformly mixing, carrying out pressure maintaining treatment at the pressure of 1.8-2.3MPa and the temperature of 130-150 ℃ for 8-10min, cooling, and reserving for later use, wherein the growth promoter is a mixture of hydrated nitric acid, hydrated cerium nitrate and N-acetyl-L-glutamic acid with the weight ratio of 1:0.7-0.8: 0.03-0.04;
and step 3: taking a microbial agent, microcrystalline cellulose and zeolite powder active carbon particles according to a formula, sterilizing the microcrystalline cellulose and the zeolite powder active carbon particles, uniformly mixing the sterilized microcrystalline cellulose and the zeolite powder active carbon particles with the microbial agent, then adding sterile water, carrying out shake culture for 40-60min under an aseptic environment with the rotating speed of 90-130r/min and the temperature of 25-35 ℃, adding sodium alginate and polyvinyl alcohol, and carrying out magnetic stirring uniformly under the condition of 100-150r/min to obtain a mixed solution for later use, wherein the weight of the sodium alginate is 0.6 times that of the microbial agent, and the weight of the polyvinyl alcohol is 5 times that of the microbial agent;
and 4, step 4: dripping the mixed solution into a mixed solution of sterile calcium chloride and sterile boric acid at a constant speed within 30-40min, crosslinking and curing for 15-25h at the temperature of 30-35 ℃ to obtain gel spheres, quickly washing the gel spheres for 2-3 times by using sterile water, transferring the gel spheres into a chitosan-sodium citrate solution, stirring for 25-30min to obtain microcapsules with the diameters of 1.5-3mM, adding the microcapsules into a 0.07mol/L sodium citrate solution for washing, freeze-drying at the temperature of-4-0 ℃ until the water content is less than or equal to 8 percent to obtain the microbial agent for wastewater treatment, wherein the microbial agent is of a double-layer microcapsule structure, the concentrations of the sterile calcium chloride solution and the sterile boric acid solution are 6 percent, the volume ratio of the sterile calcium chloride solution to the sterile boric acid solution is 1:0.53, preferably, the chitosan-sodium citrate solution is a mixed group of 0.07M sodium citrate solution, 16mg/mL chitosan solution and 0.03mM benalaxyl, the volume ratio of the R (-) -benalaxyl and the S (+) -benalaxyl in the benalaxyl is 1:1:0.005,
example 2:
and (3) testing the water treatment performance:
feed water NH3The average concentration of N was 320mg/L and the average concentration of Total Nitrogen (TN) was 601 mg/L. In the operation process, the average removal rate of ammonia nitrogen is 63%, the average removal rate of total nitrogen is only 51%, and the average removal rate of COD is 66%. The microbial inoculum prepared in example 1 is added into an aeration tank according to the adding amount of 5%, after the aeration tank runs for 30 days, the average ammonia nitrogen removal rate is 98%, the average total nitrogen removal rate is 92%, the average COD removal rate is 99%, the sludge concentration is reduced by 7% compared with that before the microbial inoculum is not added, and the adding of the microbial inoculum improves the average ammonia nitrogen removal rate in wastewater, the total nitrogen removal rate and the COD removal rate.
Claims (11)
1. A water treatment microbial agent is characterized in that: the microbial agent comprises the following components in parts by weight: 8-12 parts of microbial agent, 5-20 parts of microcrystalline cellulose, 8-18 parts of zeolite powder and 30-50 parts of activated carbon particles.
2. The microbial agent for wastewater treatment according to claim 1, wherein: the microbial agent comprises the following strains in parts by weight: 15-25 parts of bacillus licheniformis, 22-28 parts of nitrobacteria and 2-3 parts of rhodopseudomonas palustris.
3. The water treatment microbial agent as claimed in claim 1, which comprises the following components in parts by weight: 10 parts of microbial agent, 20 parts of microcrystalline cellulose, 15 parts of zeolite powder and 45 parts of activated carbon particles.
4. The microbial agent for wastewater treatment according to claim 2, wherein: the microbial agent comprises the following strains in parts by weight: 25 parts of bacillus licheniformis, 25 parts of nitrobacteria and 2 parts of rhodopseudomonas palustris.
5. The microbial agent for wastewater treatment according to claim 1, wherein: the preparation method of the microbial agent comprises the following steps:
1) respectively inoculating bacillus licheniformis, nitrobacteria and rhodopseudomonas palustris to a solid culture medium to culture to obtain an activated strain for later use;
2) compounding the thalli according to the formula amount, inoculating the thalli into a liquid culture solution, culturing to logarithmic growth phase, centrifuging, concentrating and washing the thalli to obtain a microbial agent;
3) taking microbial agent, microcrystalline cellulose and zeolite powder active carbon particles according to a formula, sterilizing the microcrystalline cellulose and the zeolite powder active carbon particles, uniformly mixing the sterilized microcrystalline cellulose and the zeolite powder active carbon particles with the microbial agent, then adding sterile water, carrying out shake culture, adding sodium alginate and polyvinyl alcohol, and uniformly stirring by magnetic force to obtain a mixed solution for later use;
4) and dropwise adding the mixed solution at a constant speed into a mixed solution of sterile calcium chloride and sterile boric acid, crosslinking and solidifying to obtain gel spheres, washing with sterile water, transferring into a chitosan-sodium citrate solution, stirring to obtain microbial preparation microspheres, adding into the sodium citrate solution, washing, and freeze-drying to obtain the microbial preparation for water treatment.
6. The method for preparing a microbial agent for water treatment according to claim 5, wherein: in the step 3, the weight of the sodium alginate is 0.6 time of that of the microbial agent, and the weight of the polyvinyl alcohol is 5 times of that of the microbial agent.
7. The method for preparing a microbial agent for water treatment according to claim 5, wherein: in the step 4, the chitosan-sodium citrate solution is a mixed group of 0.07M sodium citrate solution, 16mg/mL chitosan solution and 0.03mM benomyl, and the volume ratio of the chitosan-sodium citrate solution to the benomyl is 1:1: 0.005.
8. The method for preparing a microbial agent for water treatment according to claim 7, wherein: in the step 4, the ratio of R (-) -benalaxyl to S (+) -benalaxyl in the benalaxyl is 1: 0.04.
9. The method for preparing a microbial agent for water treatment according to claim 5, wherein: in the step 4, the concentrations of the sterile calcium chloride solution and the sterile boric acid solution are both 6%, and the volume ratio of the sterile calcium chloride solution to the sterile boric acid solution is 1: 0.53.
10. The method for preparing microbial inoculum for wastewater treatment according to claim 5, which is characterized in that: in the step 4, the crosslinking curing temperature is 37 ℃ and the time is 14 h.
11. The method for preparing microbial inoculum for wastewater treatment according to claim 5, which is characterized in that: the diameters of the microbial agents for wastewater treatment in the step 4 are all 1.5-3 mm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010787583.2A CN111747541A (en) | 2020-08-07 | 2020-08-07 | Water treatment microbial agent |
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| CN202010787583.2A CN111747541A (en) | 2020-08-07 | 2020-08-07 | Water treatment microbial agent |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112481249A (en) * | 2020-11-27 | 2021-03-12 | 天津滴碳节能科技有限公司 | Preparation method of immobilized biocatalyst |
| CN112715890A (en) * | 2020-12-25 | 2021-04-30 | 贵州统之源食品有限公司 | Immobilized pickle starter culture and application thereof |
| CN115353258A (en) * | 2022-08-29 | 2022-11-18 | 江苏利然环保科技有限公司 | Modular water purification device for improving water quality of water body |
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2020
- 2020-08-07 CN CN202010787583.2A patent/CN111747541A/en active Pending
Patent Citations (3)
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| WO2008040157A1 (en) * | 2006-09-28 | 2008-04-10 | Wuhan Probiotic Spring Biotechnology Co., Ltd. | One kind of artificial biomembrane and its preparation method |
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| CN109609406A (en) * | 2018-12-27 | 2019-04-12 | 广州市金龙峰环保设备工程股份有限公司 | A kind of slow-release microbial bacterial agent and preparation method thereof administered for black and odorous water |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112481249A (en) * | 2020-11-27 | 2021-03-12 | 天津滴碳节能科技有限公司 | Preparation method of immobilized biocatalyst |
| CN112715890A (en) * | 2020-12-25 | 2021-04-30 | 贵州统之源食品有限公司 | Immobilized pickle starter culture and application thereof |
| CN112715890B (en) * | 2020-12-25 | 2024-01-09 | 贵州统之源食品有限公司 | Immobilized pickle starter and application thereof |
| CN115353258A (en) * | 2022-08-29 | 2022-11-18 | 江苏利然环保科技有限公司 | Modular water purification device for improving water quality of water body |
| CN115353258B (en) * | 2022-08-29 | 2023-10-27 | 江苏利然环保科技有限公司 | Modularized water purifying device for improving water quality of water body |
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