CN110342651B - Microbial enzyme composite preparation, preparation method thereof and application thereof in treatment of industrial sewage or landfill leachate - Google Patents

Microbial enzyme composite preparation, preparation method thereof and application thereof in treatment of industrial sewage or landfill leachate Download PDF

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CN110342651B
CN110342651B CN201910742445.XA CN201910742445A CN110342651B CN 110342651 B CN110342651 B CN 110342651B CN 201910742445 A CN201910742445 A CN 201910742445A CN 110342651 B CN110342651 B CN 110342651B
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aerobic
anaerobic
preparation
filtrate
bacterial protease
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CN110342651A (en
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张瑞娜
刘超
傅乾
邰俊
杨韬
余召辉
曹瑞杰
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Shanghai Environmental Sanitation Engineering Design Institute Co ltd
Shanghai Institute for Design and Research on Environmental Engineering Co Ltd
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Shanghai Environmental Sanitation Engineering Design Institute Co ltd
Shanghai Institute for Design and Research on Environmental Engineering Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/06—Contaminated groundwater or leachate
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a microbial enzyme composite preparation, a preparation method thereof and application thereof in treating industrial sewage or landfill leachate, and relates to the technical field of sewage treatment. The aerobic bacterial protease and the anaerobic bacterial protease extracted from the activated sludge can provide a large amount of active enzymes for aerobic microorganisms and anaerobic microorganisms in the EM microbial inoculum, so that the initial reaction rate is improved, the initial COD removal rate of the EM microbial inoculum can be obviously improved, and the sewage environment which can possibly break the balance of the EM microbial inoculum is rapidly improved, so that the microbial enzyme composite preparation provided by the invention can be suitable for treating various industrial sewage or landfill leachate.

Description

Microbial enzyme composite preparation, preparation method thereof and application thereof in treatment of industrial sewage or landfill leachate
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a microbial enzyme composite preparation, a preparation method thereof and application thereof in treating industrial sewage or landfill leachate.
Background
With the development of industrialization and the increase of urban population, the urbanization is continuously enlarged, so that the discharge amount of water, waste water and sewage in cities is continuously increased, the shortage of water resources and the pollution of water bodies are aggravated, and the treatment of urban domestic sewage and industrial sewage or landfill leachate becomes a focus of great concern in environmental protection. In recent decades, the treatment technology of municipal sewage has been developed continuously, mainly including aerobic microorganism treatment technology, and mainly divided into two main treatment processes, namely an activated sludge process and a biofilm process. The conventional activated sludge process has poor nitrogen and phosphorus treatment effect and is not easy to reach the discharge standard, so that the aerobic treatment and the anaerobic treatment are combined during sewage treatment to further remove ammonia nitrogen.
The EM (effective microorganisms) technology is proposed by professor of professor Jia Lufu of Youki university in Japan, 20 th century, 80 th era, and the EM microbial inoculum is a compound microbial inoculum which takes photosynthetic bacteria as the center, coexists with azotobacter, propagates, adopts a special fermentation process, and mixes the carefully screened aerobic microorganisms and anaerobic microorganisms in a proper proportion to cultivate. Useful substances and secreted substances thereof produced by various microorganisms during their growth become substrates and raw materials for microbial population growth, and a complex and stable microbial system is formed by the symbiotic proliferation relationship between them, thereby performing various functions. At present, the EM microbial inoculum has good application effects in the aspects of agriculture, animal husbandry, breeding industry, environmental purification and the like.
After the EM microbial inoculum is applied to the sewage, beneficial bacteria can quickly occupy an dominant position and absorb nutrient substances (most organic substances and part of inorganic substances) in the sewage, so that the sewage is clear, the stink is eliminated, and the ecological environment is recovered. In many countries around the world, sewage treatment is carried out by using EM microbial inoculum, and Japanese, Netherlands, New Zealand, Pakistan, China, Thailand and the like are widely applied. The EM microbial inoculum is used for treating sewage and boiling water, the effluent quality is good, the biological phase is increased, the sludge yield is low, and the scum can be inhibited.
Zhang Yong et al studied the application of EM technology in the treatment of wastewater from Curcuma rhizome processing, and the results showed that EM flora can treat wastewater containing high salt, oil and cyclic compounds, and has strong resistance to high concentration sulfate (Zhang Yong, Zhang Cheng, Qicheng, et al. application of EM technology in the treatment of wastewater from Curcuma rhizome processing [ J ]. environmental science and technology, 2005 (S1): 119-120.). The combined treatment of the waste water from saponin production by EM (effective chemical oxygen demand) bacteria and aerobic sludge by Liu Jian Party, Song Feng Ming and the like is researched, and the results show that under the optimal test conditions, the removal rate of COD (chemical oxygen demand) of the waste water by EM bacteria and aerobic activated sludge in an SBR reactor reaches 97.95%, the removal rate of chromaticity reaches 66.67%, EM bacteria have strong adaptability to high COD load and high salt concentration, and can grow well in the comprehensive waste water from saponin production (Song Feng Ming, exhaling, Liu Yin. research on the treatment of the waste water from saponin production by yeast [ J ]. environmental pollution treatment technology and equipment, 2004, 5 (4): 66-69.; Ling Stone, Feng Gui, Jian Liu, and the like. The method for treating sewage by using the EM microbial inoculum is simple and convenient to operate, low in cost and good in effect of removing a large amount of organic matters in sewage, and is a safe and effective sewage treatment method.
However, the sewage to be treated has different environments, and some sewage is not suitable for the growth of the flora of the EM microbial inoculum or inhibits the growth of part of beneficial microorganisms, such as photosynthetic bacteria and the like. The unsuitable sewage environment can cause unbalance of functional flora of the EM microbial inoculum, and break the symbiotic proliferation relation of the EM microbial inoculum, so that the EM microbial inoculum can not play an effective sewage purification role. Therefore, there is a need in the art to solve the problem that EM agents cannot be used directly.
Disclosure of Invention
The invention provides a microbial enzyme composite preparation containing EM microbial inoculum, aiming at overcoming the defect that the existing EM microbial inoculum cannot adapt to partial sewage environment to cause sewage treatment failure, and the microbial enzyme composite preparation can effectively improve the initial purification rate of the EM microbial inoculum and quickly transform sewage into an environment suitable for the survival of the EM microbial inoculum.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a microbial enzyme composite preparation, which comprises EM microbial inoculum, aerobic bacterial protease and anaerobic bacterial protease.
Preferably, the mass ratio of the EM microbial inoculum to the aerobic bacterial protease to the anaerobic bacterial protease is 5-10: 2-5: 3-7.
Preferably, the dosage form of the microbial compound preparation comprises powder, granules, liquid, tablets and capsules.
The invention also provides a preparation method of the microbial enzyme composite preparation in the technical scheme, which comprises the following steps:
(1) taking sludge at the bottom of an aerobic tank and sludge at the bottom of an anaerobic tank of a sewage treatment plant, diluting the sludge with water by 5-10 times respectively, and filtering to obtain aerobic crude filtrate and anaerobic crude filtrate;
(2) filtering the aerobic coarse filtrate and the anaerobic coarse filtrate with 0.45 μm microporous filter membrane respectively to obtain aerobic fine filtrate and anaerobic fine filtrate;
(3) inoculating the aerobic refined filtrate into a fermentation medium for aerobic culture for 3-7 d to obtain aerobic fermentation liquor;
inoculating the anaerobic refined filtrate into a fermentation medium for anaerobic culture for 3-7 d to obtain anaerobic fermentation liquor;
(4) respectively centrifuging the aerobic fermentation liquor and the anaerobic fermentation liquor, respectively taking the centrifuged precipitates to perform cell disruption and alkali extraction and acid precipitation to extract protein, and obtaining aerobic bacterial protease and anaerobic bacterial protease;
(5) mixing the EM microbial inoculum, the aerobic bacterial protease and the anaerobic bacterial protease in proportion to obtain the microbial enzyme composite preparation.
Preferably, the aperture of the filter membrane for filtering in the step (1) is 1-50 μm.
Preferably, the fermentation medium in the step (3) is industrial sewage or landfill leachate.
Preferably, the method for cell disruption in step (4) is ultrasonication.
The invention also provides application of the microbial enzyme composite preparation in the technical scheme in treatment of industrial sewage or landfill leachate.
Preferably, the aerobic bacterial protease and the anaerobic bacterial protease in the microbial enzyme composite preparation are obtained by taking industrial sewage or landfill leachate to be treated as a fermentation medium.
Preferably, the microbial enzyme composite preparation has the viable count of 0.5 multiplied by 104～4×104The proportion of each cubic meter of industrial sewage or landfill leachate is added into the industrial sewage or the landfill leachate.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a microbial enzyme composite preparation, which comprises EM microbial inoculum, aerobic bacterial protease and anaerobic bacterial protease. The aerobic bacterial protease and the anaerobic bacterial protease extracted from the activated sludge can provide a large amount of active enzymes for aerobic microorganisms and anaerobic microorganisms in the EM microbial inoculum, so that the initial reaction rate is improved, the initial COD removal rate of the EM microbial inoculum can be obviously improved, and the sewage environment which can possibly break the balance of the EM microbial inoculum is rapidly improved, so that the microbial enzyme composite preparation provided by the invention can be suitable for treating various industrial sewage or landfill leachate.
The invention also provides the application of the microbial enzyme composite preparation in the technical scheme in the treatment of industrial sewage or landfill leachate, and the microbial enzyme composite preparation can be directly added into the industrial sewage or landfill leachate to be treated, so that the operation is simple and convenient, the cost is low, and the sewage treatment effect is good.
Detailed Description
The invention provides a microbial enzyme composite preparation, which comprises EM microbial inoculum, aerobic bacterial protease and anaerobic bacterial protease. In the invention, the mass ratio of the EM microbial inoculum to the aerobic bacterial protease to the anaerobic bacterial protease is preferably 5-10: 2-5: 3-7; more preferably 6-8: 3-4: 4-6. The aerobic bacterial protease and the anaerobic bacterial protease are complex enzymes and are derived from the sludge at the bottom of an aerobic pool and the sludge at the bottom of an anaerobic pool of a sewage treatment plant.
In the present invention, the effective viable count of the microbial enzyme complex preparation is preferably not less than 1X 105More preferably 1 × 105～1×106And (4) respectively. In the present invention, the formulation of the microbial composite preparation includes, but is not limited to, powders, granules, liquids, tablets and capsules. In the present invention, the microbial composite preparation may further include adjuvants including, but not limited to, fillers, stabilizers, preservatives, pH regulators, and microbial nutrients.
The invention also provides a preparation method of the microbial enzyme composite preparation in the technical scheme, which comprises the following steps:
(1) taking sludge at the bottom of an aerobic tank and sludge at the bottom of an anaerobic tank of a sewage treatment plant, diluting the sludge with water by 5-10 times respectively, and filtering to obtain aerobic crude filtrate and anaerobic crude filtrate;
(2) filtering the aerobic coarse filtrate and the anaerobic coarse filtrate with 0.45 μm microporous filter membrane respectively to obtain aerobic fine filtrate and anaerobic fine filtrate;
(3) inoculating the aerobic refined filtrate into a fermentation medium for aerobic culture for 3-7 d to obtain aerobic fermentation liquor;
inoculating the anaerobic refined filtrate into a fermentation medium for anaerobic culture for 3-7 d to obtain anaerobic fermentation liquor;
(4) respectively centrifuging the aerobic fermentation liquor and the anaerobic fermentation liquor, respectively taking the centrifuged precipitates to perform cell disruption and alkali extraction and acid precipitation to extract protein, and obtaining aerobic bacterial protease and anaerobic bacterial protease;
(5) mixing the EM microbial inoculum, the aerobic bacterial protease and the anaerobic bacterial protease in proportion to obtain the microbial enzyme composite preparation.
The method comprises the steps of taking sludge at the bottom of an aerobic tank and sludge at the bottom of an anaerobic tank of a sewage treatment plant, diluting the sludge by 5-10 times with water, and filtering to obtain aerobic coarse filtrate and anaerobic coarse filtrate. The invention aims to dilute and filter with water so as to primarily separate sludge from microorganisms in the sludge and remove large sludge. In the present invention, the dilution factor of water is a ratio of a volume of sludge at the bottom of the tank to a volume of water, and the dilution factor of water is preferably 6 to 8 times. In the invention, the aperture of the filter membrane for filtration is preferably 1-50 μm. In the present invention, the filtration method includes suction filtration.
After obtaining the aerobic coarse filtrate and the anaerobic coarse filtrate, respectively filtering the aerobic coarse filtrate and the anaerobic coarse filtrate by 0.45 mu m microporous filter membranes to obtain aerobic fine filtrate and anaerobic fine filtrate. The invention adopts a 0.45 mu m microporous filter membrane for filtration, and aims to further separate microorganisms such as bacteria and the like from other impurities in the crude filtrate. In the invention, the filtration times of the 0.45-micron microporous filter membrane are preferably 2-3 times, and the filtrates are combined when the 0.45-micron microporous filter membrane is filtered for multiple times.
After obtaining aerobic refined filtrate and anaerobic refined filtrate, inoculating the aerobic refined filtrate into a fermentation medium for aerobic culture for 3-7 days to obtain aerobic fermentation liquor; and inoculating the anaerobic refined filtrate into a fermentation medium for anaerobic culture for 3-7 d to obtain anaerobic fermentation liquor. The invention aims to enrich aerobic bacteria and anaerobic bacteria in aerobic refined filtrate and anaerobic refined filtrate which are respectively fermented by fermentation culture media. In the present invention, the temperature of the aerobic culture is preferably 25 to 35 ℃, and more preferably 28 ℃. In the present invention, the time for the aerobic culture is preferably 4 to 6 days, and more preferably 5 days. In the invention, the temperature of the anaerobic culture is preferably 25-35 ℃, and more preferably 28 ℃. In the invention, the time for anaerobic culture is preferably 4-6 days, and more preferably 5 days. In the invention, the fermentation medium is preferably industrial sewage or landfill leachate, and the industrial sewage or landfill leachate is used as the fermentation medium, so that on one hand, the culture cost can be saved, and on the other hand, aerobic bacteria or anaerobic bacteria can secrete more enzymes suitable for decomposing the industrial sewage or the landfill leachate, so as to better assist the EM microbial inoculum in sewage treatment.
After the aerobic fermentation liquor and the anaerobic fermentation liquor are obtained, the invention respectively centrifuges the aerobic fermentation liquor and the anaerobic fermentation liquor, respectively takes the centrifugalized sediment for cell disruption, alkali extraction and acid precipitation to extract protein, and obtains the aerobic bacterial protease and the anaerobic bacterial protease. In the invention, the speed of centrifugation is preferably 1500-3000 rpm, and more preferably 2000 rpm; the time for centrifugation is preferably 3-5 min. In the present invention, the method for cell disruption is preferably ultrasonication, and ultrasonication has low damage to the activity of protease. In the invention, the sediment is preferably mixed with normal saline according to the volume ratio of 1: 1-5 before the ultrasonic crushing so as to complete the ultrasonic crushing. The invention has no special limitation on the specific acid-base types of the alkali extraction and acid precipitation, and can finish the protein extraction.
After aerobic bacterial protease and anaerobic bacterial protease are obtained, the EM microbial inoculum, the aerobic bacterial protease and the anaerobic bacterial protease are mixed according to a proportion to obtain the microbial enzyme composite preparation. If the microbial enzyme composite preparation further comprises auxiliary materials, the mixture of the EM microbial agent, the aerobic bacterial protease and the anaerobic bacterial protease is mixed with the auxiliary materials to prepare different formulations, and the invention has no special limitation on the formulation.
The invention also provides application of the microbial enzyme composite preparation in the technical scheme in treatment of industrial sewage or landfill leachate. Preferably, the aerobic bacterial protease and the anaerobic bacterial protease in the microbial enzyme composite preparation are obtained by taking industrial sewage or landfill leachate to be treated as a fermentation culture medium. The invention can customize a special microbial enzyme composite preparation for industrial sewage or landfill leachate to be treated in a targeted manner, and has better treatment effect.
In the present invention, the microbial enzyme complex preparation preferably has a viable count of 0.5X 104～4×104The ratio of each cubic meter of industrial sewage or landfill leachate is added into the industrial sewage or the landfill leachate, and the viable count is more preferably 1 × 104～2×104Per cubic meter of industrial sewage or landfill leachate. The microbial composite preparation can be suitable for treating industrial sewage or landfill leachate from various sources, can be directly applied, and is convenient to use.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Self-prepared CODCrThe concentration is 3000mg/L, BOD5The concentration of the synthetic wastewater is 1200mg/L, pH value, and the synthetic wastewater has a value of 10-11.
(1) Taking 10L of aerobic tank bottom sludge and anaerobic tank bottom sludge of a sewage treatment plant in a certain Shanghai area respectively, adding 10 times of water by volume respectively, mixing, and filtering with a 30-micrometer filter membrane to obtain aerobic coarse filtrate and anaerobic coarse filtrate;
(2) filtering the aerobic coarse filtrate and the anaerobic coarse filtrate with 0.45 μm microporous filter membrane respectively to obtain aerobic fine filtrate and anaerobic fine filtrate;
(3) inoculating the aerobic refined filtrate into the synthetic wastewater for aerobic culture for 5d to obtain aerobic fermentation liquor; inoculating the anaerobic refined filtrate into synthetic wastewater for anaerobic culture for 7d to obtain anaerobic fermentation broth;
(4) and (3) respectively centrifuging the aerobic fermentation liquor and the anaerobic fermentation liquor (the centrifugal rotation speed is 2500rpm, and the centrifugation is carried out for 3min), adding physiological saline into the centrifuged precipitates according to the volume ratio of 1: 1, and then carrying out ultrasonic cell disruption to obtain the disrupted aerobic fermentation liquor and the disrupted anaerobic fermentation liquor. Respectively adding 0.1M NaOH solution into the crushed aerobic fermentation liquor and the crushed anaerobic fermentation liquor until the pH value is 12.0, standing for 2h, then adding 0.1M HCl solution until the pH value is 3.5, standing for 2h again, and then filtering, wherein precipitates are aerobic bacterial protease and anaerobic bacterial protease;
(5) mixing EM microbial inoculum (commercially available EM microbial inoculum stock solution), aerobic bacterial protease and anaerobic bacterial protease according to the mass ratio of 6: 4 to obtain the microbial enzyme composite preparation.
Example 2
Self-prepared CODCrThe concentration is 3000mg/L, BOD5The concentration of the synthetic wastewater is 1200mg/L, pH value, and the synthetic wastewater has a value of 10-11.
(1) Taking 10L of aerobic tank bottom sludge and anaerobic tank bottom sludge of a sewage treatment plant in a certain Shanghai area respectively, adding 5 times of water by volume respectively, mixing, and filtering with a 5-micrometer filter membrane to obtain aerobic coarse filtrate and anaerobic coarse filtrate;
(2) filtering the aerobic coarse filtrate and the anaerobic coarse filtrate with 0.45 μm microporous filter membrane respectively to obtain aerobic fine filtrate and anaerobic fine filtrate;
(3) inoculating the aerobic refined filtrate into the synthetic wastewater for aerobic culture for 7d to obtain aerobic fermentation liquor; inoculating the anaerobic refined filtrate into synthetic wastewater for anaerobic culture for 7d to obtain anaerobic fermentation broth;
(4) and (3) respectively centrifuging the aerobic fermentation liquor and the anaerobic fermentation liquor (the centrifugal rotation speed is 1500rpm, and the centrifugation is 4min), adding physiological saline into the centrifuged precipitates according to the volume ratio of 1: 2, and then carrying out ultrasonic cell disruption to obtain the disrupted aerobic fermentation liquor and the disrupted anaerobic fermentation liquor. Respectively adding 0.1M NaOH solution into the crushed aerobic fermentation liquor and the crushed anaerobic fermentation liquor until the pH value is 12.0, standing for 2h, then adding 0.1M HCl solution until the pH value is 3.5, standing for 2h again, and then filtering, wherein precipitates are aerobic bacterial protease and anaerobic bacterial protease;
(5) mixing EM microbial inoculum (commercially available EM microbial inoculum stock solution), aerobic bacterial protease and anaerobic bacterial protease according to the mass ratio of 7: 3: 6 to obtain the microbial enzyme composite preparation.
Example 3
Self-prepared CODCrThe concentration is 3000mg/L, BOD5The concentration of the synthetic wastewater is 1200mg/L, pH value, and the synthetic wastewater has a value of 10-11.
The microbial enzyme complex preparation prepared in example 1 and EM microbial inoculum were mixed in accordance with viable count of 1X 105The sewage/cubic meter is put into 10L of synthetic wastewater, which is marked as an experimental group and a control group, and the wastewater is continuously stirred for 72 hours and blown and aerated once every 2 hours.
1. Taking water samples of the experimental group and the control group which are treated for 2 hours for microscopic examination, and observing the biological phases by adopting a biological microscopic image analysis system.
Microscopic examination results show that the water sample of the control group for 2h contains no photosynthetic bacteria and filamentous bacteria, while the water sample of the test group for 2h contains photosynthetic bacteria and filamentous bacteria. Earlier researches find that when the EM microbial inoculum is cultured by using the synthetic wastewater, photosynthetic bacteria and filamentous bacteria are lost, which is probably because the self-made synthetic wastewater does not meet the growth of certain microbial populations in the EM microbial inoculum, so that the mutual symbiotic effect of EM microbial populations is broken. The microbial enzyme composite preparation added with the aerobic bacterial protease and the anaerobic bacterial protease realizes no loss of flora in the same synthetic wastewater, and overcomes the problem that the flora balance of EM microbial inoculum is broken in partial industrial sewage or landfill leachate.
2. Respectively taking water samples of an experimental group and a control group which are treated for 2h, 24h and 72h, and determining COD (chemical oxygen demand) in each water sample by using a potassium dichromate methodCrConcentration and calculating COD at different treatment timesCrThe results of the removal rate are shown in Table 1.
TABLE 1 COD at different treatment timesCrRemoval rate
Group of Treatment of COD for 2hCrRemoval rate COD for treating 24hCrRemoval rate Treatment of 72h CODCrRemoval rate
Experimental group 23.7％ 58.1％ 82.2％
Control group 10.2％ 53.0％ 76.8％
As can be seen from the data in Table 1, the COD at 2h was observed in the experimental group compared to the control groupCrThe removal rate is obviously improved, which shows that the microbial enzyme composite preparation provided by the invention can effectively improve the purification efficiency during initial treatment, quickly improve the environment of sewage to be treated and enable the EM microbial inoculum to be more easily adapted to the sewage environment. Experiment group for treating COD for 24h and 72hCrThe removal rate is obviously higher than that of a control group, which shows that the microbial composite preparation provided by the invention has better effect on sewage treatment.
Example 4
Getting COD of a place in Shanghai CityCrThe concentration is 7000-9000 mg/L, BOD5The concentration of the landfill leachate is 4500-6000 mg/L, pH, and the value is 5-6. The microbial enzyme composite preparation prepared in the example 1 is used for treating for 24 hours, and COD in the landfill leachate after treatment is detectedCrThe results show the COD after 24hCrThe removal rate was 51.6%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (8)

1. The preparation method of the microbial enzyme compound preparation is characterized by comprising the following steps of:
(1) taking sludge at the bottom of an aerobic tank and sludge at the bottom of an anaerobic tank of a sewage treatment plant, diluting the sludge with water by 5-10 times respectively, and filtering to obtain aerobic crude filtrate and anaerobic crude filtrate;
(2) respectively filtering the aerobic coarse filtrate and the anaerobic coarse filtrate by using 0.45-micrometer microporous filter membranes to obtain aerobic fine filtrate and anaerobic fine filtrate;
(3) inoculating the aerobic refined filtrate into a fermentation medium for aerobic culture for 3-7 d to obtain aerobic fermentation liquor;
inoculating the anaerobic refined filtrate into a fermentation medium for anaerobic culture for 3-7 d to obtain anaerobic fermentation liquor;
(4) centrifuging the aerobic fermentation liquor and the anaerobic fermentation liquor respectively, and taking the centrifuged precipitates for cell disruption and alkali extraction and acid precipitation to extract protein to obtain aerobic bacterial protease and anaerobic bacterial protease;
(5) mixing the EM microbial inoculum, the aerobic bacterial protease and the anaerobic bacterial protease in proportion to obtain the microbial enzyme composite preparation.
2. The preparation method according to claim 1, wherein the mass ratio of the EM microbial inoculum, the aerobic bacterial protease and the anaerobic bacterial protease is 5-10: 2-5: 3-7.
3. The preparation method according to claim 1 or 2, wherein the microbial enzyme complex preparation is in the form of powder, granules, liquid, tablets and capsules.
4. The method according to claim 1, wherein the pore size of the filter membrane for filtration in step (1) is 1 to 50 μm.
5. The method according to claim 1, wherein the fermentation medium in step (3) is industrial sewage or landfill leachate.
6. The method for preparing the cell of claim 1, wherein the method for disrupting the cells of step (4) is ultrasonication.
7. The use of the microbial enzyme complex preparation prepared by the preparation method of claim 1 in the treatment of industrial sewage or landfill leachate.
8. The use of claim 7, wherein the microbial enzyme complex preparation has a viable count of 0.5 x 104～4×104The proportion of each cubic meter of industrial sewage or landfill leachate is added into the industrial sewage or the landfill leachate.
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