CN102531199A - Method for treating monosodium glutamate wastewater by utilizing lipomyces starkeyi - Google Patents
Method for treating monosodium glutamate wastewater by utilizing lipomyces starkeyi Download PDFInfo
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- CN102531199A CN102531199A CN2012100089529A CN201210008952A CN102531199A CN 102531199 A CN102531199 A CN 102531199A CN 2012100089529 A CN2012100089529 A CN 2012100089529A CN 201210008952 A CN201210008952 A CN 201210008952A CN 102531199 A CN102531199 A CN 102531199A
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Abstract
The invention relates to a method for treating monosodium glutamate wastewater by utilizing lipomyces starkeyi, and the method comprises the following steps of: (1) inoculating lipomyces starkeyi onto an activation medium to carry out activation culture, so as to obtain activated cells; (2) inoculating the activated cells into a liquid seed culture medium to carry out seed culture, so as to obtain liquid seeds; (3) inoculating the liquid seeds into a fermentation lipid-producing culture medium to carry out fermentation culture, so as to obtain fermentation liquor; and (4) carrying out centrifugal separation on the fermentation liquor, and collecting thallus precipitate, wherein the supernatant is the treated monosodium glutamate wastewater. In the method provided by the invention, lipomyces starkeyi is used for treating waste water, a great amount of microbial oil can be obtained, and the waste water can be effectively treated while environmental pollution is reduced.
Description
Technical field
The present invention relates to a kind of gourmet powder waste water that utilizes and produce greasy method, be specifically related to utilize this Da Shi saccharomyces oleaginosus to handle the method for gourmet powder waste water, belong to technical field of biotechnology.
Background technology
Gourmet powder waste water as a kind of waste water of foodstuffs industry, is a kind of organic waste water of high density. it has characteristics such as acid strong, high COD, high BOD, high sulfate radical, high thalline content, low temperature; Through detecting, contain COD3 in every liter of gourmet powder waste water, 7389 ± 1000mg/L; The residual sugar amount is 5.03633 ± 0.5g/L, and protein contnt is 12.97359 ± 0.2g/L, and pH=2.86 ± 0.25; Simultaneously, the msg output of China occupies the first in the world, and the high-concentration waste water in the glutamate production process is treated as not; A large amount of directly dischargings can cause serious environmental problem, destroy the eubiosis.Traditional gourmet powder waste water recycling treatment has increased the reasons such as operation cost of enterprise because the operation cost expense is higher, and the practical application achievement of waste water reclaiming seldom.Therefore, we need seek a kind of inexpensive method and handle gourmet powder waste water.
Oleaginous microorganism is meant can produce greasy mikrobe; There are yeast, mould, bacterium and algae etc. all to contain oleaginous microorganism; Wherein the yeast of eucaryon, mould and algae can be synthesized with vegetables oil and formed similar triglyceride level, the then synthetic special lipid of the bacterium of protokaryon.What study morely at present is yeast, algae and mould.The mikrobe that is used to produce pufas now mainly is algae, bacterium and fungi, because bacterium yields poorly, so mainly concentrate on algae and fungi at present.Common oleaginous yeast has: shallow white Cryptococcus (Cryptococcus albidus), curved Cryptococcus (Cryptococcus albidun), this Da Shi saccharomyces oleaginosus (Lipomyces), Trichosporon pullulans (Trichospiron pullulans), produce oil saccharomyces oleaginosus (Lipomy slipo fer), gluing rhodotorula (Rhodotorula giutinis), type red winter of yeast full (Rhodosporidium toru loides) etc.Common produce oil mould has: native mould (Asoergullus terreus), purple paralysed ergot (Clavicepspurpurea), jowar pleat are embraced ustilago (Tolyposporium), Mortierella alpina (Mortierella alpina), Mortierella isabellina (Mortierella isabellina) etc.Common produce oil marine alga has: diatom (diatom) and tenaculat Habenaria (Spirulina).
Utilize oleaginous microorganism to handle gourmet powder waste water; Not only can reduce production costs; Obtain a large amount of microbial oils, but also can alleviate or effectively handle waste water pollution problem, reduce pollution environment; Help environment protection and continuous development of society economy, environment protection and recycling economy are all had positive effect.Therefore, making full use of oleaginous microorganism processing gourmet powder waste water alleviation china natural resources problem is significant.
Summary of the invention
The present invention is directed to the deficiency of prior art, a kind of method of utilizing this Da Shi saccharomyces oleaginosus to handle gourmet powder waste water is provided, this method can obtain the by product grease simultaneously.
Utilize this Da Shi saccharomyces oleaginosus to handle the method for gourmet powder waste water, may further comprise the steps:
(1) this Da Shi saccharomyces oleaginosus be inoculated in carry out activation culture on the activation medium, Kiwi;
(2) Kiwi that step (1) is made is inoculated in and carries out seed culture in the liquid seed culture medium, obtains liquid seeds;
(3) liquid seeds that step (2) is made is inoculated in fermentation and produces in the fat substratum, and shaking culture 4d～5d under the condition of 28～32 ℃ of temperature, 150～200r/min obtains fermented liquid;
(4) get the fermented liquid that step (3) makes, through spinning, the separating thallus deposition, supernatant is the gourmet powder waste water after the processing.
It is to add glucose 70～100g in every liter of gourmet powder waste water that the fat substratum is produced in said fermentation.
Activation medium in the said step (1), component is (g/L) as follows: glucose 20, peptone 10, yeast extract paste 10, agar 20.
Activation culture condition in the said step (1) is: under the condition of 28～32 ℃ of temperature, cultivate 3d-4d.
Liquid seed culture medium in the said step (2), component is (g/L) as follows: glucose 70, (NH
4)
2SO
42, KH
2PO
41, MgSO
47H
2O 0.5～1.0, yeast extract paste 0.5, pH=5.8～6.0.
Seed culture condition in the said step (2) is: shaking culture 1d under the condition of 28～32 ℃ of temperature, 150～200r/min.
Inoculum size in the said step (3) is 8～12wt%.
Spinning condition in the said step (4) is: centrifugal 5～10min under the 8000rpm.
Said substratum all needs at 121 ℃ of following saturation steam sterilization 20min.
This Da Shi saccharomyces oleaginosus of the present invention (Lipomyces starkeyi) can be buied by market for this area oleaginous yeast commonly used.For example this Da Shi saccharomyces oleaginosus of culture presevation GIM2.142 is preserved in microbial strains preservation center, Guangdong Province.
What the present invention did not limit in detail all gets final product by state of the art.
Beneficial effect
1, the present invention is fermentation raw material with the gourmet powder waste water, realizes utilization of waste material, resources circulation, advantage such as comprehensive cost is low;
2, the inventive method is utilized oleaginous microorganism---and this Da Shi saccharomyces oleaginosus is handled waste water, not only can obtain a large amount of microbial oils, but also can effectively handle waste water, reduces environmental pollution.
Embodiment
Below in conjunction with embodiment the present invention is done further elaboration, but institute of the present invention protection domain is not limited thereto.
This Da Shi saccharomyces oleaginosus (Lipomyces starkeyi) among the embodiment (bacterium numbering: GIM2.142) purchase microbial strains preservation center in Guangdong Province.Gourmet powder waste water among the embodiment is taken from Chiping County, Liaocheng Shandong Gourmet Powder Factory, through detecting, and the substance C OD 3.7389 ± 1000mg/L that contains in every liter of gourmet powder waste water, the residual sugar amount is 5.03633 ± 0.5g/L, protein contnt 12.97359 ± 0.2g/L.
Embodiment
Method with this Da Shi saccharomyces oleaginosus processing gourmet powder waste water may further comprise the steps:
(1) this Da Shi saccharomyces oleaginosus is inoculated on the activation medium activation culture 3d under the condition of 30 ℃ of temperature, Kiwi; Activation medium, component be (g/L) as follows: glucose 20, peptone 10, yeast extract paste 10, agar 20.
(2) Kiwi that step (1) is made is inoculated in the liquid seed culture medium, and shaking culture 1d under the condition of 28～30 ℃ of temperature, 150r/min obtains liquid seeds; Liquid seed culture medium, component be (g/L) as follows: glucose 70, (NH
4)
2SO
42, KH
2PO
41, MgSO
47H
2O 1, yeast extract paste 0.5, pH=6.0.
(3) liquid seeds that step (2) is made is inoculated in the fermentation product fat substratum by 10wt% and carries out fermentation culture, and shaking culture 4d under the condition of 30 ℃ of temperature, 200r/min obtains fermented liquid;
(4) get fermented liquid that step (3) makes in centrifuge tube, centrifugal 5min under 8000rpm, the separating thallus deposition, supernatant is the gourmet powder waste water after the processing.
It is to add glucose 80g in every liter of gourmet powder waste water that the fat substratum is produced in said fermentation.
Gourmet powder waste water after the processing detects:
DNS colorimetric method for determining residual sugar amount, National Standard Method GB11914-89 measures COD, and nitrogen determination is measured protein contnt.
The index of waste water detects after treatment, and residual sugar amount, protein and COD are respectively 0.036g/L, 8.62g/L and 0.9362mg/L, and wherein the clearance of protein and COD is respectively up to 32.66% and 74.96%.
Thalline living weight (DW) is measured:
Get fermented liquid 10mL, centrifugal 5min under 8000rpm collects bacterial sediment; With deionized water wash 2 times, centrifugal 5min under 8000rpm, supernatant discarded; Thalline is put into thermostat container; Dry to constant weight in 105 ℃, be cooled to the weight that weighing disk after the room temperature adds dry mycelium, the weight that the weight that weighing disk adds dry mycelium deducts weighing disk draws dry cell weight.
The numerical value of thalline living weight is 4.61g/L.
Greasy extraction determination of yield: chloroform methanol method
Get under the fermented liquid 10mL 3000r/min behind the centrifugal 10min, bacterial sediment with the 4mL hexane after, adopt the chloroform-methanol method to measure.1: 2 (v/v) CHCl that adds 6mL
3: CH
3OH shaking table vibration 2min adds 2mL CHCl
3Vibration 2min adds 2mL deionized water vibration 2min again, and the centrifugal 5min of 3000r/min gets chloroform layer, and volatilization is removed chloroform and got grease.
The numerical value of grease yield is 1.14g/L, and the numerical value of fat content is 24.73wt%.
Claims (8)
1. utilize this Da Shi saccharomyces oleaginosus to handle the method for gourmet powder waste water, it is characterized in that, may further comprise the steps:
(1) this Da Shi saccharomyces oleaginosus be inoculated in carry out activation culture on the activation medium, Kiwi;
(2) Kiwi that step (1) is made is inoculated in and carries out seed culture in the liquid seed culture medium, obtains liquid seeds;
(3) liquid seeds that step (2) is made is inoculated in fermentation and produces in the fat substratum, and shaking culture 4d～5d under the condition of 28～32 ℃ of temperature, 150～200r/min obtains fermented liquid;
(4) get the fermented liquid that step (3) makes, through spinning, the separating thallus deposition, supernatant is the gourmet powder waste water after the processing.
2. the method for claim 1 is characterized in that, the activation medium in the said step (1), and component is following, and unit is g/L: glucose 20, peptone 10, yeast extract paste 10, agar 20.
3. the method for claim 1 is characterized in that, the activation culture condition in the said step (1) is: under the condition of 28～32 ℃ of temperature, cultivate 3d～4d.
4. the method for claim 1 is characterized in that, the liquid seed culture medium in the said step (2), and component is following, and unit is g/L:
Glucose 70, (NH
4)
2SO
42, KH
2PO
41, MgSO
47H
2O 0.5～1.0, yeast extract paste 0.5, pH=5.8～6.0.
5. the method for claim 1 is characterized in that, the seed culture condition in the said step (2) is: shaking culture 4d～5d under the condition of 28～32 ℃ of temperature, 150～200r/min.
6. the method for claim 1 is characterized in that, it is to add glucose 70～100g in every liter of gourmet powder waste water that the fat substratum is produced in said fermentation.
7. the method for claim 1 is characterized in that, the inoculum size in the said step (3) is 8～12wt%.
8. the method for claim 1 is characterized in that, the spinning condition in the said step (4) is: centrifugal 5～10min under the 8000rpm.
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| CN2012100089529A CN102531199A (en) | 2012-01-12 | 2012-01-12 | Method for treating monosodium glutamate wastewater by utilizing lipomyces starkeyi |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105110557A (en) * | 2015-08-24 | 2015-12-02 | 华南农业大学 | Method for treating kitchen wastewater through flocculation flotation-oil production microorganisms |
| CN114162978A (en) * | 2021-12-14 | 2022-03-11 | 昆明理工大学 | Application of cryptococcus oxytetracycline in wastewater treatment |
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| CN102080119A (en) * | 2009-11-26 | 2011-06-01 | 北京化工大学 | Method for producing oil by mixed culture of yeast and alga |
| US20110252696A1 (en) * | 2010-04-14 | 2011-10-20 | Solazyme, Inc. | Fuel And Chemical Production From Oleaginous Yeast |
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2012
- 2012-01-12 CN CN2012100089529A patent/CN102531199A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102080119A (en) * | 2009-11-26 | 2011-06-01 | 北京化工大学 | Method for producing oil by mixed culture of yeast and alga |
| US20110252696A1 (en) * | 2010-04-14 | 2011-10-20 | Solazyme, Inc. | Fuel And Chemical Production From Oleaginous Yeast |
Non-Patent Citations (2)
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| 王华等: "啤酒生产废水培养斯达油脂酵母发酵产油脂培养基的优化", 《中国油脂》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105110557A (en) * | 2015-08-24 | 2015-12-02 | 华南农业大学 | Method for treating kitchen wastewater through flocculation flotation-oil production microorganisms |
| CN105110557B (en) * | 2015-08-24 | 2018-09-18 | 华南农业大学 | A kind of method of flocculation-air floating-oleaginous microorganism processing kitchen waste water |
| CN114162978A (en) * | 2021-12-14 | 2022-03-11 | 昆明理工大学 | Application of cryptococcus oxytetracycline in wastewater treatment |
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