CN102642993B - Alcohol fermentation wastewater treatment method - Google Patents
Alcohol fermentation wastewater treatment method Download PDFInfo
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- CN102642993B CN102642993B CN 201210133467 CN201210133467A CN102642993B CN 102642993 B CN102642993 B CN 102642993B CN 201210133467 CN201210133467 CN 201210133467 CN 201210133467 A CN201210133467 A CN 201210133467A CN 102642993 B CN102642993 B CN 102642993B
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Abstract
The invention discloses an alcohol fermentation wastewater treatment method. The method includes the steps: removing solid impurities in alcohol fermentation wastewater, adjusting the pH (potential of hydrogen) value of the alcohol fermentation wastewater to range from 5.0 to 8.0, inoculating oil yeast seed liquid according to an inoculums size of 2.5-20%(v/v), aerobically fermenting for two to five days at the fermentation temperature ranging from 25 DEG C to 35 DEG C, collecting yeasts in the wastewater after fermentation is completed, and subjecting the wastewater to subsequent treatment according to discharging requirements. Using the method for treatment of alcohol fermentation wastewater is high in treatment efficiency and COD (chemical oxygen demand) removal rate and can omit a long-time strain domestication period required by traditional anaerobic or aerobic activated sludge treatment methods while produce the yeasts rich of oil, protein, nucleic acid, lycopene and other high-added-value components, and only simple and conventional aerobic fermentation equipment is needed instead of technical equipment which is expensive and complex in use and required by the anaerobic activated sludge method and the like, so that the method is simple in process and low in cost. Additionally, no nutrient substance needs to be added in the whole fermentation process, and accordingly the fermentation period is short.
Description
Technical field:
The present invention relates to a kind for the treatment of process of alcohols fermentation waste water, be specifically related to a kind of remarkable method that reduces alcohols fermentation waste water COD the yeast of production high added value simultaneously thalline of saccharomyces oleaginosus fermentation of utilizing.
Background technology:
In recent years, along with the exhaustion of petroleum resources, the mankind are increasing to the exploration of new forms of energy.Can produce a large amount of biological liquid fuels as butanols, ethanol etc. by microbial fermentation technology, be an effective outlet that solves current energy dilemma.After fermentation ends, by simple distillation technique, can reclaim the products such as butanols, ethanol and acetone in fermented liquid.Yet remaining fermented liquid is rich in the organic acids such as butyric acid, acetic acid, its COD often, more than 4000mg/L, therefore needs follow-up wastewater treatment just can reach emission standard.
Saccharomyces oleaginosus (oleaginous yeast), can utilize the yeast of the high producing microbial grease of various carbon source materials and tropina, starts in recent years to be used to the processing of various trade effluents.For example, the big seminar of the Tan Tian of Beijing University of Chemical Technology can process with rhodotorula glutinis the trade effluents such as monosodium glutamate, starch, papermaking, and produces microbial oil (Bioresour.Technol.2010,101:6092-5).Venkata Subhash etc. utilize grease microorganism process corn cob waste water (Bioresour.Technol., 2011,102 (19), 9286-9290).Yousuf etc. utilize saccharomyces oleaginosus process sweet oil waste water etc. (J.Agric.Food Chem., 2010,58 (15), 8630-8635).Different from above-mentioned fermentation waste water, the alcohols fermentation waste water is rich in the organic acids such as butyric acid, acetic acid, and COD is often more than 4000mg/L.As a rule, use internal-circulation anaerobic reactor (IC) and utilize anaerobic activated sludge can process this organic acid fermentation waste water that is rich in and can reach certain treatment effect.But this technique needs complicated internal-circulation anaerobic reactor, and construction cost is higher.And active sludge need to reach the domestication of several months just can reach treatment effect, thereby processing efficiency is lower.
At present, there are no document or patent report, use saccharomyces oleaginosus to process the alcohols fermentation waste water.
Summary of the invention:
The purpose of this invention is to provide a kind of can effectively process alcohols fermentation waste water, processing efficiency high, process easy, cost is low, the treatment time is short, after fermentation ends, can collect the treatment process of alcohols fermentation waste water of the yeast thalline of high value added products such as being rich in grease, protein, nucleic acid, Lyeopene.
The treatment process of alcohols fermentation waste water of the present invention, it is characterized in that, comprise the following steps, remove solid impurity in the alcohols fermentation waste water, the pH value of regulating again the alcohols fermentation waste water is 5.0～8.0, then by inoculum size, be 2.5～20% (v/v) access saccharomyces oleaginosus seed liquor, leavening temperature is 25～35 ℃, and fermentation time is 2～5 days, carry out aerobic fermentation, after fermentation ends, collect the yeast thalline in waste water, waste water carries out subsequent disposal according to emission request.
In described removal alcohols fermentation waste water, solid impurity is preferably removed solid impurity in the alcohols fermentation waste water by filtering or precipitating.
Described saccharomyces oleaginosus can be any in rhodotorula glutinis (Rhodotorula glutinis), trichosporon cutaneum (Trichosporon cutaneum), circle red winter spore yeast (Rhodosporidium toruloides), Lipomyces starkeyi (Lipomyces starkeyi), light white latent ball yeast (Cryptococcus albidus), Trichosporon dermatis and Trichosporn coremiiforme.
Described saccharomyces oleaginosus seed liquor is preparation by the following method preferably, by saccharomyces oleaginosus bacterial classification access activation medium, and 25～35 ℃, activation culture 24～48 hours, described activation medium is glucose or wood sugar 20g/L, peptone 10g/L, yeast powder 10g/L, and surplus is water.
Yeast thalline in described collection waste water preferably is rich in the yeast thalline of grease, albumen, nucleic acid, Lyeopene and other high added value composition by filtration or centrifugal collection.
Beneficial effect of the present invention is as follows:
1. process the alcohols fermentation waste water according to method of the present invention, not only processing efficiency is high, the COD clearance is high, can also exempt as traditional anaerobism, required long-time strain domestication stage of aerobic activated sludge treatment process, produce the yeast thalline that is rich in grease, albumen, nucleic acid, Lyeopene and other high added value composition simultaneously, there is application prospect preferably.
2. saccharomyces oleaginosus is processed the aerobic fermentation equipment that alcohols waste water only needs simple and regular, without as anaerobic activated sludge method etc. use the processing unit (as inner circulation reactor etc.) of expensive complexity, technique is simple, with low cost.
3. whole fermenting process is without adding any nutritive substance, and fermentation period is shorter, and product yeast microorganism collection is simple.
Embodiment:
Following examples are to further illustrate of the present invention, rather than the restriction to inventing.
Embodiment 1:
Utilize rhodotorula glutinis (Rhodotorula glutinis) to process butylic fermentation waste water
By in the activation medium of rhodotorula glutinis (Rhodotorula glutinis) access 100ml, 25 ℃, activation culture 48 hours, obtain the rhodotorula glutinis seed liquor, described activation medium is: glucose 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
By the butylic fermentation waste water filtering, remove solid impurity in butylic fermentation waste water, the initial COD of butylic fermentation waste water after removal impurity is about 25000mg/L, the initial pH value of regulating waste water with acetic acid is 5.0, inoculum size access rhodotorula glutinis seed liquor with 10% (v/v), 28 ℃ of lower aerobic fermentations are after 3 days, yeast thalline in centrifugal collection butylic fermentation waste water, waste water carries out subsequent disposal according to emission request, the COD clearance of the butylic fermentation waste water after the rhodotorula glutinis fermentative processing can reach 75%, and the yeast scale of construction is 5.5g/L.
Embodiment 2
Utilize trichosporon cutaneum (Trichosporon cutaneum) to process butylic fermentation waste water
By in the activation medium of trichosporon cutaneum (Trichosporon cutaneum) access 50ml, 35 ℃, activation culture 24 hours, obtain the trichosporon cutaneum seed liquor, described activation medium is: wood sugar 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
By the butylic fermentation wastewater sedimentation, remove solid impurity in butylic fermentation waste water, the initial COD of butylic fermentation waste water after removal impurity is about 23000mg/L, the initial pH value of regulating waste water with calcium hydroxide is 8.0, inoculum size access trichosporon cutaneum seed liquor with 5% (v/v), 28 ℃ of lower aerobic fermentations are after 4 days, yeast thalline in centrifugal collection butylic fermentation waste water, waste water carries out subsequent disposal according to emission request, the COD clearance of the butylic fermentation waste water after the trichosporon cutaneum fermentative processing can reach 80%, and the yeast scale of construction is 6.5g/L.
Embodiment 3
Utilize the red winter spore yeast of circle (Rhodosporidium toruloides) to process ethanol fermentation waste water
To justify in the activation medium of red winter spore yeast (Rhodosporidium toruloides) access 80ml, 30 ℃, activation culture 36 hours, obtain the red winter spore yeast starter liquid of circle, described activation medium is: wood sugar 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
By the butylic fermentation wastewater sedimentation, remove solid impurity in butylic fermentation waste water, the initial COD of butylic fermentation waste water after removal impurity is about 12000mg/L, the initial pH value of regulating waste water with ammoniacal liquor is 6.5, the red winter spore yeast starter liquid of inoculum size access circle with 8% (v/v), 28 ℃ of lower aerobic fermentations are after 2 days, yeast thalline in centrifugal collection butylic fermentation waste water, waste water carries out subsequent disposal according to emission request, the COD clearance of the butylic fermentation waste water after the red winter spore yeast fermentation of circle is processed can reach 70%, and the yeast scale of construction is 3.8g/L.
Embodiment 4
Utilize Lipomyces starkeyi (Lipomyces starkeyi) to process ethanol fermentation waste water
By in the activation medium of Lipomyces starkeyi (Lipomyces starkeyi) access 150ml, 30 ℃, activation culture 36 hours, obtain the Lipomyces starkeyi seed liquor, described activation medium is: wood sugar 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
By the butylic fermentation waste water filtering, remove solid impurity in butylic fermentation waste water, the initial COD of butylic fermentation waste water after removal impurity is about 21000mg/L, the initial pH value of regulating waste water with sodium hydroxide is 6.5, inoculum size access Lipomyces starkeyi seed liquor with 20% (v/v), 30 ℃ of lower aerobic fermentations are after 5 days, yeast thalline in centrifugal collection butylic fermentation waste water, waste water carries out subsequent disposal according to emission request, the COD clearance of the butylic fermentation waste water after the Lipomyces starkeyi fermentative processing can reach 78%, and the yeast scale of construction is 4.9g/L.
Embodiment 5
Utilize light white latent ball yeast (Cryptococcus albidus) to process butylic fermentation waste water
By in the activation medium of light white latent ball yeast (Cryptococcus albidus) access 125ml, 30 ℃, activation culture 36 hours, obtain the light white latent ball yeast seed liquor, described activation medium is: wood sugar 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
By the butylic fermentation waste water filtering, remove solid impurity in butylic fermentation waste water, the initial COD of butylic fermentation waste water after removal impurity is about 24000mg/L, the initial pH value of regulating waste water with sodium hydroxide is 7.5, inoculum size access light white latent ball yeast seed liquor with 12% (v/v), 28 ℃ of lower aerobic fermentations are after 4 days, yeast thalline in centrifugal collection butylic fermentation waste water, waste water carries out subsequent disposal according to emission request, the COD clearance of the butylic fermentation waste water after the light white latent ball yeast fermentative processing can reach 65%, and the yeast scale of construction is 3.2g/L.
Embodiment 6
Utilize Trichosporon dermatis to process ethanol fermentation waste water
By in the activation medium of Trichosporon dermatis access 25ml, 30 ℃, activation culture 36 hours, obtain Trichosporon dermatis seed liquor, described activation medium is: glucose 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
By the butylic fermentation waste water filtering, remove solid impurity in butylic fermentation waste water, the initial COD of butylic fermentation waste water after removal impurity is about 18000mg/L, the initial pH value of regulating waste water with sodium hydroxide is 7.8, inoculum size access Trichosporon dermatis seed liquor with 2.5% (v/v), 25 ℃ of lower aerobic fermentations are after 4.5 days, yeast thalline in centrifugal collection butylic fermentation waste water, waste water carries out subsequent disposal according to emission request, the COD clearance of the butylic fermentation waste water after Trichosporon dermatis fermentative processing can reach 82%, the yeast scale of construction is 4.5g/L.
Embodiment 7
Utilize Trichosporn coremiiforme to process butylic fermentation waste water
By in the activation medium of Trichosporn coremiiforme access 80ml, 30 ℃, activation culture 36 hours, obtain Trichosporn coremiiforme seed liquor, described activation medium is: glucose 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
By the butylic fermentation waste water filtering, remove solid impurity in butylic fermentation waste water, the initial COD of butylic fermentation waste water after removal impurity is about 28000mg/L, the initial pH value of regulating waste water with sodium hydroxide is 8.0, inoculum size access Trichosporn coremiiforme seed liquor with 7.5% (v/v), 35 ℃ of lower aerobic fermentations are after 5 days, yeast thalline in centrifugal collection butylic fermentation waste water, waste water carries out subsequent disposal according to emission request, the COD clearance of the butylic fermentation waste water after Trichosporn coremiiforme fermentative processing can reach 76%, the yeast scale of construction is 5.1g/L.
Embodiment 8
Utilize Trichosporn coremiiforme to process butylic fermentation waste water
By in the activation medium of Trichosporn coremiiforme access 80ml, 30 ℃, activation culture 28 hours, obtain Trichosporn coremiiforme seed liquor, described activation medium is: glucose 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
By the butylic fermentation waste water filtering, remove solid impurity in butylic fermentation waste water, the initial COD of butylic fermentation waste water after removal impurity is about 4000mg/L, the initial pH value of regulating waste water with sodium hydroxide is 6.5, inoculum size access Trichosporn coremiiforme seed liquor with 20% (v/v), 30 ℃ of lower aerobic fermentations are after 5 days, yeast thalline in centrifugal collection butylic fermentation waste water, waste water carries out subsequent disposal according to emission request, the COD clearance of the butylic fermentation waste water after Trichosporn coremiiforme fermentative processing can reach 80%, the yeast scale of construction is 0.81g/L.
Claims (4)
1. the treatment process of an alcohols fermentation waste water, it is characterized in that, comprise the following steps, remove solid impurity in the alcohols fermentation waste water, the pH value of regulating again the alcohols fermentation waste water is 5.0～8.0, then by inoculum size, be volume ratio 2.5～20% access saccharomyces oleaginosus seed liquor, leavening temperature is 25～35 ℃, and fermentation time is 2～5 days, carry out aerobic fermentation, after fermentation ends, collect the yeast thalline in waste water, waste water carries out subsequent disposal according to emission request;
Described saccharomyces oleaginosus is any in rhodotorula glutinis (Rhodotorula glutinis), trichosporon cutaneum (Trichosporon cutaneum), circle red winter spore yeast (Rhodosporidium toruloides), Lipomyces starkeyi (Lipomyces starkeyi), light white latent ball yeast (Cryptococcus albidus), Trichosporon dermatis and Trichosporn coremiiforme.
2. the treatment process of alcohols fermentation waste water according to claim 1, is characterized in that, in described removal alcohols fermentation waste water, solid impurity is to remove solid impurity in the alcohols fermentation waste water by filtering or precipitating.
3. the treatment process of alcohols fermentation waste water according to claim 1, it is characterized in that, described saccharomyces oleaginosus seed liquor is to prepare by the following method, by in saccharomyces oleaginosus bacterial classification access activation medium, 25～35 ℃, activation culture 24～48 hours, described activation medium is glucose or wood sugar 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.
4. the treatment process of alcohols fermentation waste water according to claim 1, is characterized in that, the yeast thalline in described collection waste water is by filtering or centrifugal collection.
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| CN103911297A (en) * | 2014-02-28 | 2014-07-09 | 中国科学院南海海洋研究所 | Rhodosporidium toruloides Y0 and application thereof |
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| CN114686535A (en) * | 2020-12-31 | 2022-07-01 | 中国科学院广州能源研究所 | Method for pretreating alcohol fermentation wastewater to improve conversion rate of grease yeast and regulate composition of grease fatty acid |
| CN112553095B (en) * | 2021-01-09 | 2022-03-22 | 仲恺农业工程学院 | Compound microbial inoculum for treating high-concentration kitchen wastewater and preparation method thereof |
| CN113956116A (en) * | 2021-12-03 | 2022-01-21 | 西北工业大学深圳研究院 | Method for preparing microbial probiotic fertilizer by fermenting kitchen waste liquid |
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| IT1393929B1 (en) * | 2008-12-18 | 2012-05-17 | Eni Spa | PROCEDURE FOR THE PRODUCTION OF BIO-OIL FROM BIOMASS |
| WO2011130573A1 (en) * | 2010-04-14 | 2011-10-20 | Solazyme, Inc. | Fuel and chemical production from oleaginous yeast |
| CN101857884B (en) * | 2010-06-10 | 2012-11-28 | 江苏联海生物科技有限公司 | Method for producing ethanol by using acetone-butanol fermentation wastewater as ingredient |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103911297A (en) * | 2014-02-28 | 2014-07-09 | 中国科学院南海海洋研究所 | Rhodosporidium toruloides Y0 and application thereof |
| CN103911297B (en) * | 2014-02-28 | 2015-10-28 | 中国科学院南海海洋研究所 | A kind of Rhodosporidium toruloides Y0 and application thereof |
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