CN110386676A - A kind of double tank up flow type biological filter tank systems and its method for handling organic matter - Google Patents

A kind of double tank up flow type biological filter tank systems and its method for handling organic matter Download PDF

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CN110386676A
CN110386676A CN201910737597.0A CN201910737597A CN110386676A CN 110386676 A CN110386676 A CN 110386676A CN 201910737597 A CN201910737597 A CN 201910737597A CN 110386676 A CN110386676 A CN 110386676A
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reactor
sulfate
tank
flow type
biological filter
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刘波文
刘济忠
石井裕之
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DONGGUAN TAITENG EP MATERIAL TECHNOLOGY Co Ltd
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DONGGUAN TAITENG EP MATERIAL TECHNOLOGY Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/101—Sulfur compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The application belongs to field of wastewater treatment, more particularly to a kind of double tank up flow type biological filter tank systems and its method for handling organic matter, it is characterized by: the second waste water energy is drawn into first reactor bottom, it can be connected at the top of first reactor with second reactor bottom, and second reactor lower part is connected with nitrification tank, first reactor and second reactor are made of acrylic cylinder, bio-carrier made of 2/3 filled polypropylene foam of first reactor cylinder top, bio-carrier made of 2/3 filled polypropylene foam of second reactor cylinder top, liquid flows out at the top of second reactor after second reactor processing, and test developing experiment is carried out according to particular step and particular substrate content.The reduction of acid-utilising salt and sulphur source denitrogenation of the present invention are combined, can be reached using particular substrate content and quick and precisely be tested.

Description

A kind of double tank up flow type biological filter tank systems and its method for handling organic matter
Technical field
The application belongs to field of wastewater treatment, and in particular to a kind of double tank up flow type biological filter tank systems and its processing are organic The method of object.
Background technique
Due to the eutrophication problem of enclosed waters, the nutriment removed in sewage becomes extremely important.In general, biological Denitrogenation mainly uses organic substance to carry out heterotrophic denitrification bacterial treatment as electron donor, but when organic carbon source deficiency, needs Add organic matter (operation cost is high).Therefore, for the denitrogenation of waste water of shortage organic carbon source, it has been proposed that many application sulphur Denitrifying bacteria removal lacks the denitrogenation method of organic carbon source sewage.This thiobacillus denitrificans (Thiobacillus It denitirificans is) a kind of using reduction sulphur source as electron donor, the autotrophy that nitrate is cultivated as electron acceptor Bacterium.But this method needs to cooperate the nitrate concentration in waste water, adjusts the reduced forms sulphur such as sodium thiosulfate and elemental sulfur The additive amount in source and the pH of waste water, such operation control become extremely complex.It is well known that sulfate reducing bacteria is nature In the anaerobic bacteria that is widely present.In the case of anaerobic or few oxygen, it can be using the organic matter of metal surface as carbon source, and benefit The energy of existence, In are obtained from redox reaction by sulfate reduction at hydrogen sulfide with the hydrogen generated in bacterial biof iotalm The hydrogen sulfide that waste water disposal facility generates in engineering, the problems such as concrete erosion can be caused.Meanwhile this sulfate reducing bacteria In anaerobic digestion to the influence of methane production, the generation of methane can make trichobacteria mass propagation in anaerobic digestion, cause Activated sludge bulking.And sulfate reduction promotes the decomposition of organic matter, the in particular to generation of acetic acid.Due to this sulfate Reduction can be by keeping anaerobic to be easy to keep its activity, while being allowed to generate vulcanization using this sulfate reducing bacteria Object supplies back segment thiobacillus denitrificans as ability source.
Summary of the invention
In order to solve the above technical problem, the present invention provides one kind to be combined by sulfate reduction and sulphur source denitrogenation, be adopted The double tank up flow type biological filter tank systems that can be quick and precisely tested with particular substrate content and its method for handling organic matter.
The technical solution of the present invention is as follows:
A kind of double tank up flow type biological filter tank systems, the second waste water energy are drawn into first reactor bottom, the first reaction It can be connected at the top of device with second reactor bottom, and second reactor lower part is connected with nitrification tank, first reactor and second Reactor is made of acrylic cylinder, bio-carrier made of 2/3 filled polypropylene foam of first reactor cylinder top, the Bio-carrier made of two reactor cylinder top, 2/3 filled polypropylene foam, liquid is anti-from second after second reactor processing Answer outflow at the top of device.
Further, nitrification tank is arranged in third reactor, be connected at the top of second reactor with third reactor so that Residual organic matter matter and sulfide are nitrified and are aoxidized.
A kind of method of double tank up flow type biological filter tank systems processing organic matters, follows the steps below,
The first step, using the first waste water culture sulfate reducing bacteria is raised and train in first reactor, in second reactor It is interior that thiobacillus denitrificans are cultivated by matrix C；
Second waste water is pumped into first reactor by second step, being capable of phase with second reactor bottom at the top of first reactor Even, liquid flows into second reactor after first reactor processing, while second reactor lower part is connected with nitrification tank, a certain concentration Nitrate enter second reactor from nitrification tank；
Sulfate reduction is wherein carried out in first reactor, sulfate reduction generates sulfide and acetic acid, and second is anti- Answer in device while carrying out heterotrophic denitrification and sulphur source oxidation process denitrification denitrification；Thiobacillus denitrificans in second reactor are utilized from the The sulfide of one reactor supply carries out denitrogenation, and the heterotrophic denitrification bacterium use in second reactor is supplied from first reactor Acetic acid carry out denitrogenation.
Further, the first waste water be artificial wastewater, and raise and train the first waste water time be one month；Second waste water is artificial Waste water.
Further, the artificial wastewater contains CH3COOK, polyprotein peptone, yeast extract, NaHCO3、KCl、 MgSO4·7H2O、CaCl2、KH2PO4、MgCl2Quality ratio be 200:400:40:71:175:157:51:91:61.
Further, the C/N ratio of liquid is 1.8-3.6 after first reactor processing, and nitrification tank is to second reactor bottom The nitrate concentration of portion's addition is 250-500mg/L, and nitrate is NaNO in the digester3。
Further, first reactor sulfate reducing bacteria produces acetic acid with organic acid；Second reactor grows sulfuric acid Salt reducing bacteria, and second reactor sulfate-reducing activity is higher by first reactor sulfate-reducing activity 30%；Second is anti- The activity for answering thiobacillus denitrificans in device is the 1/5 of heterotrophic denitrification bacterium.
Further, it is added to matrix A and sodium molybdate 960mg/L in the sulfate reduction rate measurement, mesostroma A contains CH3COOK, polyprotein peptone, yeast extract, NaHCO3、KCl、MgSO4·7H2O、CaCl2、KH2PO4、MgCl2Quality ratio For 200:400:40:71:175:157:51:91:61.
Further, it is added to matrix B in the measurement of sulphur source oxidation process denitrification denitrification rate, mesostroma B contains CH3COOK, polyprotein peptone, yeast extract, NaHCO3、KCl、CaCl2、KH2PO4、MgCl2Quality ratio be 200:400: 40:71:175:51:91:61。
Further, matrix C contains NaHCO3、KCl、CaCl2、KH2PO4、MgCl2Quality ratio be 71:175:51: 91:61；Matrix C is added in heterotrophic denitrification speed measurement.
As shown in Figure 1, first reactor: (valeric acid) hydrogenesis and acetogenesis reaction equation:
CH3CH2CH2CH2COOH+2H2O→CH3CH2COOH+CH3COOH+2H2
CH3CH2COOH+2H2O→CH3COOH+2H2+2CO2
Sulfate reduction: SO4 2-+2NH4 +→S+N2+4H2O
G ° of Δ of the reaction is -47.8KJ/mol, and infers that this biological respinse can combine nitrous acid salt form anaerobism Ammoxidation reaction obtains, reaction equation are as follows:
3SO4 2-+4NH4 +→3S2-+4NO2 -+4H2O+8H+
3S2-+2NO2 -+8H+→3S+N2+4H2O
2NO2 -+2NH4 +→2N2+4H2O
Second reactor: synchronized desulfuring denitrogenation
Part reaction sulfate reducing bacteria carries out
Sulphur source aoxidizes denitrification: 5S+6NO3 -+2H2O→5SO4 2-+4H++3N2
Heterotrophic denitrification: make carbon source using organic acid and realize heterotrophic denitrification
Third reactor: aerobic nitrification reaction: aerobic nitrification reaction: NH4 ++2O2→NO3 -+H2O+2H+
The invention has the benefit that
1. the degraded processing of organic matter is carried out according to sulfate reduction under anaerobic condition, than common liquor recycling It is few that mode (nitrate reduction and interdependent the organic matter of oxygen breathing decompose) generates sludge.Meanwhile utilizing sulfate reducing bacteria The outstanding organic matter having decomposes promotion ability, and the removal efficiency for the Organic substance in water that gives up improves.In order to handle water system sulphur grain Generation, and strengthen to the advantages of load change of nitrogen is also this treatment process.Of the invention being used by device overcomes The generation of hydrogen sulfide.This treatment process is suitble to sewer treatment facility and purification tank etc., for livestock products draining etc. organic matters, The wastewater treatment of nitrogen high concentration is also applicable in very much.The sludge quantity generated in the reactor is few, does not need during whole service Sludge is discharged.
2. the artificial wastewater that the present invention adds specific matrix A, matrix B, matrix C and certain content makes later data pair It is more significant than (compared with the existing technology), convenient for making theoretical analysis to data phenomenon.
Detailed description of the invention
Fig. 1 is sulfate reduction-sulphur source oxidation process denitrification denitrification process schematic illustration；
Fig. 2 is the liquid schematic diagram that experimental setup and sampled point acquire；
Fig. 3 a is the change procedure of sulfate concentration；
Fig. 3 b is the change procedure of nitrate concentration；
Fig. 4 a be embodiment 1 when reactor in water quality organic matter variation diagram；
Fig. 4 b be embodiment 2 when reactor in water quality organic matter variation diagram；
Fig. 5 is the experimental result of microbial biomass under anaerobic in first reactor；
Fig. 6 a is the heterotrophic denitrification experimental result of microbial biomass under anaerobic in second reactor；
Fig. 6 b is the sulphur denitrogenation experimental result of microbial biomass under anaerobic in second reactor；
Fig. 7 is the relational graph of " the also commercial weight of sulfate " and " output of acetic acid relevant to sulfate reduction "；
The relational graph that Fig. 8 passes through " the nitric acid amount being consumed according to heterotrophic denitrification bacterium " and " consumption of acetic acid "；
Fig. 9 a is that sulfate reducing bacteria, thiobacillus denitrificans, heterotrophic denitrification bacterium longitudinally inhabit figure in reactor；
Fig. 9 b is the schematic diagram that the concentration gradient of organic matter, nitrogen and sulphur source is generated in biomembrane；
Fig. 9 c is the schematic diagram that sulfate reducing bacteria nearby grows thiobacillus denitrificans under anaerobic condition；
Wherein: 1: first reactor, 2: second reactor, 3: third reactor, 4: artificial wastewater, 5: nitrification tank, 6: poly- Bio-carrier made of acryl foam, 7: the first centrifugal pumps, 8: the second centrifugal pumps, 9: first reactor influent, 10: the first Liquid after reactor for treatment, 11: second reactor bottom liquid, 12: liquid after second reactor processing.
Specific embodiment
In conjunction with attached drawing, the present invention is further illustrated.
A kind of double tank up flow type biological filter tank systems, it is characterised in that: the first waste water energy is drawn into first reactor bottom It is interior, it can be connected at the top of first reactor with second reactor bottom, and second reactor lower part is connected with nitrification tank, first is anti- Device and second reactor is answered to be made of acrylic cylinder, made of 2/3 filled polypropylene foam of first reactor cylinder top Bio-carrier, bio-carrier made of 2/3 filled polypropylene foam of second reactor cylinder top, the first waste water energy being cleaned It is enough to be exported at the top of second reactor.
Experimental method
As shown in Fig. 2, being used using two experiments to type bio-filter bed reactor.Two reactors are by diameter 140mm is highly made of the acrylic cylinder of 300mm, bio-carrier made of 2/3 filled polypropylene foam of cylinder top.For A large amount of sulfate reducing bacterias are accumulated in first reactor, need to raise and train about one month using artificial wastewater in table 1.In order to Second reactor assembles thiobacillus denitrificans, is raised and train using the matrix C in table 1.After raising and train, two reactors are connected, it will Artificial wastewater injects first reactor in table 1, then liquid flows into second reactor after first reactor is handled, while from Second reactor lower part addition nitrate (setting nitrification tank is arranged in third reactor).First reactor, the second reaction Total void tower residence time of device is 3 hours.
Then, in the nitrate concentration of the lower part of second reactor addition embodiment 1, embodiment 2, and make the second reaction Device is intake to be implemented according to the C/N ratio of embodiment 1, embodiment 2.
Liquid 10, second reactor bottom liquid after taken at regular intervals first reactor influent 9, first reactor processing 11, liquid 12 after second reactor processing, and carry out water analysis.Analysis project include sulfate, nitrate, nitrite, Organic acid and sulfide.In addition, removing equipment in the last day of experiment, the microorganism in reactor is taken out, and according to as follows The measuring of step progress the sulfate reduction rate, sulphur source oxidation process denitrification denitrification rate and heterotrophic denitrification rate:
The sludge in reactor is centrifugated first, the sludge of centrifuge separation is then put into multiple bottle (volumes of hatching In 100mL), its concentration is made to reach 1000mg/L, and fill nitrogen and replace oxygen in bottle completely, clogs bottleneck.In 20 DEG C of items Under part, magnetic stirrer culture is used.In stir culture, opens one bottle at regular intervals and analyzed.Sulfate Matrix A and sodium molybdate 960mg/L is added in rate of reduction, adds matrix B, the anti-nitre of sulphur source oxidation process denitrification in heterotrophic denitrification rate Change and add matrix C in rate, mesostroma A, matrix B, matrix C referring to table 1, the sulfate reduction rate, heterotrophic denitrification rate, Sulphur source oxidation process denitrification denitrification rate is referring to table 2.
Embodiment 1 (i.e. run1)
Nitrate concentration is 250mg/L；C/N is 3.6 in the waste water of entrance.Remaining is identical as experimental method.
Embodiment 2 (i.e. run2)
Nitrate concentration is 500mg/L；C/N is 1.7 in the waste water of entrance.Remaining is identical as experimental method.
Experimental result and analysis
First reactor and second reactor are independently raised and train one month, the sulfate almost all in first reactor It disappears, is able to confirm that sulfate reduction just in good progress.The sulfate concentration of second reactor whole process treatment liquid Increase, good gas can be observed and generated, first reactor and second reactor are connected.Fig. 3 a, 3b display connection two anti- After answering device, sulfate and nitrate changes with time situation in reactor.From bringing into operation about 20 days, in first reactor Sulfate almost disappear, and observe there is increase in a second reactor.This shows that sulphur occurs in first reactor Hydrochlorate reduction reaction generates sulfide, is oxidized again in a second reactor.On the other hand, add from second reactor lower part The nitrate added, is nearly no detectable in treatment liquid, this shows sulphur source oxidation process denitrification denitrification just in second reactor Middle progress.But since the sulfate concentration in second reactor does not return to the concentration of influent, and sulfide It remains in liquid after treatment, therefore embodiment 2 is attempted to will be added to 2 times of the nitrate concentration increase of second reactor.This When, the sulfate concentration for the treatment of liquid be restored to it is consistent with influent concentration, and after sulfide yet and does not flow out to processing In liquid, but observe that nitrate remains in liquid after treatment.
Fig. 4 a, Fig. 4 b are the organic matter variation diagram of water quality in reactor.It can observe in first reactor with sulfuric acid Salt reduction reaction protein is converted into acetic acid.According in theoretical active sludge sulfate with from the organic matter knot of protein derived It closes and generates acetic acid, the experimental result is completely the same therewith.On the other hand, in the second reactor, as the reduction of nitrate anion is (de- Nitrogen reaction), " increase of sulfate radical " and " reduction of acetic acid " both reactions can be observed simultaneously.This represent anti-second Device is answered to react using the heterotrophic denitrification of the sulphur source oxidation process denitrification anti-nitration reaction of sulfide and the organic carbon source that acetic acid is utilized The simultaneous fact.Sulfate concentration increases in " embodiment 2 " and nitrate concentration reduces, and wherein nitrate concentration increases Adding is more than " embodiment 1 ", but detects that the TOC of 20mg/L is more than " embodiment 1 " in processing water.Nitrate concentration reduces, but TOC ratio " embodiment 1 " the high 20mg/L detected in processing water.The sludge quantity very little generated in the reactor, entire Discharge sludge is not needed during operation.
Fig. 5 is the experimental result of microbial biomass under anaerobic in first reactor.With 75mg/L (0.75mM) The reduction of sulfate will increase out 25mg/L (0.76mM) sulfide.It can be observed that sulfate reduction is measuring opinion The progress of change, and as a result, can assume that the reduction speed of this sulfate is 0 secondary response, this regulation " sulfuric acid from this Salt reduction activation ".
Fig. 6 a is the heterotrophic denitrification experimental result of microbial biomass under anaerobic in second reactor, which show As the experiment of electronics donor addition peptone and acetic acid, it is able to confirm that the reduction with acetic acid, nitrate anion is reduced.Herein The increase of nitrate can hardly be observed, therefore, it is considered that heterotrophic denitrification preferentially occurs under the experiment condition.In addition, due to It is only capable of slightly observing the accumulation of nitrite after 2 hours, therefore by the reduced rate conduct of " nitrate nitrogen+nitrite nitrogen " " heterotrophic denitrification rate ".
Fig. 6 b is the sulphur denitrogenation experimental result of microbial biomass under anaerobic in second reactor, is supplied as electronics Donor is added to sulfide.From the point of view of this experimental result, with the reduction of nitrate anion, sulfate, which generates, to be increased, nitrite Almost disappear；Thiobacillus denitrificans carry out denitrification reaction according to formula below:
5HS-+8NO3 -+3H+→5SO4 2-+4N2+4H2O…(1)
In this experiment, it with the reduction of nitric acid 85mg/L (1.3mM), generates sulfate 73mg/L (0.76mM).This The generation of sulfate is slightly fewer than theoretical value (amount discusses value 0.81mM), illustrates the anti-nitre of heterotrophism of sludge (microorganism) self decomposed Change reaction while carrying out.Therefore, advancing the speed for sulfate will be turned to be scaled that " sulphur source oxidation process denitrification is anti-according to chemical formula (1) Rate of nitrification ".Table 2 reflects that " the sulfate reduction rate " of two reactors, " heterotrophic denitrification rate ", " sulphur source oxidation is de- Nitrogen denitrification rate ".
It is 0.05~2.5mgSO from the sulfate reduction rate in general activated sludge4/ gMLSS ﹒ hr, it can be seen that this The sulfate-reducing activity of two reactors of secondary experiment is very high.Meanwhile it being lacked in the sulfate-reducing activity of second reactor Sulfate reduction performance 30%-33% higher than the sulfate-reducing activity of first reactor under the conditions of oxygen.In second reactor It observes high-sulfate reduction activation, sulfur cycle is formed by sulfate reducing bacteria and sulphur denitrifying bacteria.On the other hand, sulphur source The activity of oxidation process denitrification bacterium (thiobacillus denitrificans) is the 1/5 of heterotrophic denitrification bacterium.
When carrying out the batch experiment of anaerobic condition, the molybdate (inhibition of sulfate reducing bacteria is added in matrix Agent) as the result is shown be Fig. 5.According to the addition of this molybdate, it is able to observe that sulfate reduction is totally constrained.Meanwhile The incrementss of acetic acid decline, and propionic acid accumulation amount increases.
It is acetic acid that sulfate reducing bacteria, which is chemically reacted from following two by propionic acid:
1) sulfate reducing bacteria directly absorbs propionic acid and is oxidized to (Desulfobulbus when acetic acid propionicus)
4CH3CH2COO-+3SO4 2-→4CH3COO-+4HCO3 -+H++3HS-…(2)
2) when hydrogen ion and acetic acid coexist, (most of sulfate reducing bacterias can use hydrogen ion, remove Desulfobacter
Except acetoxidance, D.latus)
CH3CH2COO-+3H2O→CH3COO-+HCO3 -+H++3H2…(3)
SO4 2-+4H2+H+→HS-+4H2O…(4)
Such as either with or without consumption hydrogen ion in reaction equation (4), then reaction equation (3) would not occur.When reaction equation (3) and instead When formula (4) being answered to combine, it becomes and reaction equation (2).With the reduction of 3 sulfate ions, 4 propionates produce 4 second Acid ion.And in this reaction, sulfate reducing bacteria directly converts propionic acid, and it is identical also to can use hydrogen ion progress Reaction.
Fig. 7 illustrates " sulfate reduction amount " and " output of acetic acid relevant to sulfate reduction " in this experiment Relationship.
In batch experiment, the second when production quantity (Δ Ace (Mo-)) of acetic acid subtracts addition molybdenum under conditions of not adding molybdenum The production quantity of acid (after Δ Ace (Mo+), is estimated as acetic acid production quantity involved in sulfate reducing bacteria (Δ Ace (SRB)) (i.e. Δ Ace (SRB)=Δ Ace (Mo-)-Δ Ace (Mo+) is tested).In addition, as described above, in first reactor, with The reduction reaction of sulfate observe the generation of acetic acid, acetic acid production quantity involved in treatment reactor sulfate reduction is as follows Regulation.
Firstly, seeking the acetic acid formation speed k of not interdependent sulfate reduction from the addition experiment of the molybdenum of Fig. 5.Secondly, from processing Production quantity (Δ Ace of the residence time presumption of the hydrology of device in the not related acetic acid of sulfate reduction of first reactor (SRB-)=kt).Next, being determined by subtracting the value from the acetic acid actual production (Δ Ace) in first reactor It is related to the acetic acid production quantity (Δ Ace (SRB)=Δ Ace- Δ Ace (SRB-)) of sulfate reduction.Dotted line in figure assumes that sulphur Molded Line of the hydrochlorate reduction according to reaction equation (2) under a situation arises.It coincide from experimental result and this Molded Line, sulfuric acid can be estimated Salt reducing bacteria has correlation according to the generation of reaction equation (2) acetic acid.In general activated sludge sulfate reducing bacteria movement and This result is similar, this characteristic is considered as the characteristic of the sulfate reducing bacteria of sewage origin.
On the other hand, use acetic acid that can be indicated as the heterotrophic denitrification of nutrient source by reaction equation (5):
5CH3COO-+8NO3 -+8H+=5CO2+5HCO3 -+4N2+9H2O…(5)
Sulphur source oxidation process denitrification anti-nitration reaction and heterotrophic denitrification reaction carry out simultaneously in reactor.According to equal reaction equations (1) nitric acid of sulphur denitrogenation consumption is determined by the incrementss of sulfate.Fig. 8 shows the nitric acid of heterotrophic denitrification bacterium consumption Amount, and the consumption of itself and acetic acid is compared.Also it illustrates in batch experiment together, also shows denitrogenation amount and acetic acid Consumption.
Since experimental result is consistent with the Molded Line that anti-nitration reaction occurs according to reaction equation (5) is assumed, can speculate anti- The heterotrophic denitrification bacterium in device is answered mainly to react using acetic acid.This indicates the sulfate being utilized in first reactor The sulfide and acetic acid generated is restored, is steadily made in the sulphur source oxidation process denitrification and heterotrophic denitrification of second reactor With.
This experiment, it is thus identified that the excellent heterotrophic denitrification activity of second reactor and sulphur source oxidation process denitrification denitrification are living Property.Sulphur source oxidation process denitrification denitrification is inhibited under the conditions of the nitrate of embodiment 1 is insufficient, it is believed that heterotrophic denitrification is excellent First.This is G ° of Δ=- 12.4 for the sulphur source oxidation process denitrification that thermodynamically reaction equation (1) indicates, the heterotrophism that (5) formula indicates is anti- Δ G °=- 23.74 of nitrification.However, although organic matter is remaining, it is de- that sulphur source oxidation occurs completely under conditions of embodiment 2 Nitrogen denitrification, and activity is very high, it can thus be appreciated that both heterotrophic denitrification activity and sulphur source oxidation process denitrification activity are total in reactor It deposits.
On the other hand, there are in second reactor of nitric acid (under anoxic conditions), it is thus identified that high sulfate reduction Activity.The thing that original nitrate reduction denitrogenation and sulfate reduction carry out simultaneously, is the inexplicable phenomenon of thermodynamics, following public Shown in formula, generally in active sludge treatment, the presence of nitric acid can inhibit the reduction of sulfate:
1/8SO4 2-+19/16H++e-=1/16H2S+1/16HS-+1/2H2G ° of O Δ=5.085 ... (6)
1/5NO3 -+6/5H++e-=1/10N2+3/5H2G ° of O Δ=17.128 ... (7)
This is that sulfate reducing bacteria carries out fermentating metabolism or in a dormant state in the processor.Even if in anaerobic digestion In there are sulfate, it is well known that thermodynamically unfavourable methane fermentation in long period operation normally has precedence over sulfate reduction, And sulfate reduction is also to occur in the presence of oxygen.
1/8CO2+H++e-=1/8CH4+1/4H2G ° of O Δ=5.763 ... (8)
1/4O2+H++ e-=1/2H2O Δ G °=- 18.675 ... (9)
Equally in this experiment, it is believed that sulfate reduction occurs in the presence of nitrate, and is locating It manages and forms sulphur redox cycle in device.If forming sulphur source circulation, the sulphur that reuse is generated by sulfate reducing bacteria Compound, and organic matter is decomposed.
This phenomenon can be assumed by following three in makeshift device to illustrate:
(1) sulfate reducing bacteria, sulphur denitrifying bacteria, heterotrophic denitrification bacterium inhabit (figure along longitudinal direction in the reactor 9a).That is: heterotrophic denitrification bacterial multiplication is consumed the middle part as low concentration in available organic matter in bottom of device Position proliferation thiobacillus denitrificans, sulfate reducing bacteria are bred on the device top (Fig. 9 a) that nitrogen disappears.
(2) concentration gradient (Fig. 9 b) of organic matter, nitrogen and sulphur source is generated in biomembrane.That is, biofilm surface Heterotrophic denitrification bacterium, the sulphur source denitrifying bacteria in the middle part of film, sulfate reducing bacteria is present in film and carrier contact field domain.
(3) in narrow space, sulfate reducing bacteria nearby grows thiobacillus denitrificans (Fig. 9 c) under anaerobic condition.
In fact, one or several phenomenons in these three combine, biofacies is constituted.
The composition of artificial wastewater and each matrix in 1 batch experiment of table
2 denitrification rates of table look at table
The above is only presently preferred embodiments of the present invention, is not intended to limit the present invention in any form, though So the present invention has been disclosed as a preferred embodiment, and however, it is not intended to limit the invention, any technology people for being familiar with this patent Member without departing from the scope of the present invention, when the technology contents using above-mentioned prompt make it is a little change or be modified to The equivalent embodiment of equivalent variations, but anything that does not depart from the technical scheme of the invention content, it is right according to the technical essence of the invention Any simple modification, equivalent change and modification made by above embodiments, in the range of still falling within the present invention program.

Claims (10)

1. a kind of double tank up flow type biological filter tank systems, it is characterised in that: the second waste water energy is drawn into first reactor bottom, It can be connected at the top of first reactor with second reactor bottom, and second reactor lower part is connected with nitrification tank, the first reaction Device and second reactor are made of acrylic cylinder, raw made of 2/3 filled polypropylene foam of first reactor cylinder top Object carrier, bio-carrier made of 2/3 filled polypropylene foam of second reactor cylinder top, liquid after second reactor processing It is flowed out at the top of from second reactor.
2. a kind of double tank up flow type biological filter tank systems according to claim 1, it is characterised in that: nitrification tank setting is the In three reactors, it is connected with third reactor at the top of second reactor so that residual organic matter matter and sulfide carry out nitrification and oxygen Change.
3. a kind of method of double tank up flow type biological filter tank systems processing organic matters according to claim 1, feature exist In: it follows the steps below,
The first step is led in second reactor using the first waste water culture sulfate reducing bacteria is raised and train in first reactor Cross matrix C culture thiobacillus denitrificans；
Second waste water is pumped into first reactor, can be connected at the top of first reactor with second reactor bottom by second step, the Liquid flows into second reactor after one reactor for treatment, while second reactor lower part is connected with nitrification tank, certain density nitre Hydrochlorate enters second reactor from nitrification tank；
Sulfate reduction is wherein carried out in first reactor, sulfate reduction generates sulfide and acetic acid, second reactor In carry out heterotrophic denitrification and sulphur source oxidation process denitrification denitrification simultaneously；Thiobacillus denitrificans in second reactor utilize anti-from first The sulfide for answering device to supply carries out denitrogenation, and the heterotrophic denitrification bacterium in second reactor uses the second supplied from first reactor Acid carries out denitrogenation.
4. the method for double tank up flow type biological filter tank systems processing organic matters according to claim 3, it is characterised in that: the One waste water be artificial wastewater, and raise and train the first waste water time be one month；Second waste water is artificial wastewater.
5. the method for double tank up flow type biological filter tank systems processing organic matters according to claim 4, it is characterised in that: institute It states artificial wastewater and contains CH3COOK, polyprotein peptone, yeast extract, NaHCO3、KCl、MgSO4·7H2O、CaCl2、KH2PO4、 MgCl2Quality ratio be 200:400:40:71:175:157:51:91:61.
6. the method for double tank up flow type biological filter tank systems processing organic matters according to claim 3, it is characterised in that: the The C/N ratio of liquid is 1.8-3.6 after one reactor for treatment, and nitrification tank is to the nitrate concentration that second reactor bottom is added 250-500mg/L, nitrate is NaNO in the digester3。
7. the method for double tank up flow type biological filter tank systems processing organic matters according to claim 3, it is characterised in that: the One reactor sulfate reducing bacteria produces acetic acid with organic acid；Second reactor grows sulfate reducing bacteria, and second is anti- Device sulfate-reducing activity is answered to be higher by first reactor sulfate-reducing activity 30%；The work of thiobacillus denitrificans in second reactor Property is the 1/5 of heterotrophic denitrification bacterium.
8. the method for double tank up flow type biological filter tank systems processing organic matters according to claim 3, it is characterised in that: sulphur It is added to matrix A and sodium molybdate 960mg/L in the measurement of hydrochlorate rate of reduction, mesostroma A contains CH3COOK, polyprotein peptone, ferment Female extract, NaHCO3、KCl、MgSO4·7H2O、CaCl2、KH2PO4、MgCl2Quality ratio be 200:400:40:71: 175:157:51:91:61。
9. the method for double tank up flow type biological filter tank systems processing organic matters according to claim 3, it is characterised in that: sulphur It is added to matrix B in the measurement of source oxidation process denitrification denitrification rate, mesostroma B contains CH3COOK, polyprotein peptone, yeast extract Object, NaHCO3、KCl、CaCl2、KH2PO4、MgCl2Quality ratio be 200:400:40:71:175:51:91:61.
10. the method for double tank up flow type biological filter tank systems processing organic matters according to claim 3, it is characterised in that: Matrix C contains NaHCO3、KCl、CaCl2、KH2PO4、MgCl2Quality ratio be 71:175:51:91:61；Heterotrophic denitrification speed Matrix C is added in rate measurement.
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Application publication date: 20191029