CN109730093B - Aphid repellent agent and preparation method and application thereof - Google Patents
Aphid repellent agent and preparation method and application thereof Download PDFInfo
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- CN109730093B CN109730093B CN201811629756.7A CN201811629756A CN109730093B CN 109730093 B CN109730093 B CN 109730093B CN 201811629756 A CN201811629756 A CN 201811629756A CN 109730093 B CN109730093 B CN 109730093B
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Abstract
The invention relates to the field of biopesticides, and in particular relates to an aphid repellent agent as well as a preparation method and application thereof. The aphid bacteria-expelling agent comprises microzyme (Saccharomyces) CGMCC No.3278 and Rhodococcus (Rhodococcus) CGMCC No. 3276. The aphid repellent agent can obviously reduce the incidence rate of aphids and simultaneously promote the yield increase of crops.
Description
Technical Field
The invention relates to the field of biopesticides, and in particular relates to an aphid repellent agent as well as a preparation method and application thereof.
Background
Aphids are important pests on plants such as wheat, fruits and vegetables, and due to the extremely high occurrence and propagation speed of the aphids, the aphids can not only directly eat plant juice, but also spread various plant viruses, and cause serious loss on the yield and quality of the wheat, the fruits and the vegetables.
At present, chemical control is mainly adopted for aphid control in fact, because aphid propagation speed is extremely high, and other methods are difficult to control plant diseases and insect pests efficiently and quickly due to various reasons. The conventional pesticide application method of chemical control is to carry out whole-plant spraying in the aphid emergence period, and the side effect is that natural enemies are directly killed and the environment is polluted, and the food safety is seriously affected by the overproof residues caused by the current situation that chemical pesticides are excessively depended on and the use is unreasonable. Therefore, in order to reduce a plurality of serious negative effects caused by pesticides, the prevention and control of plant pests such as wheat, fruits, vegetables and the like urgently needs to develop a pollution-free prevention and control technology.
Disclosure of Invention
The invention aims to provide an aphid repellant.
It is a further object of the present invention to provide a method for reducing the incidence of aphids.
The aphid inoculant provided by the invention comprises microzyme (Saccharomyces) CGMCC No.3278 and Rhodococcus (Rhodococcus) CGMCC No. 3276.
The aphid inoculant provided by the invention is characterized in that the volume ratio of yeast (Saccharomyces) CGMCC No.3278 to Rhodococcus (Rhodococcus) CGMCC No.3276 is 2: 1.
The invention also provides a method for preparing the aphid bacteria repellent, which comprises the step of enlarging culture of microzyme (Saccharomyces) CGMCC No.3278) and Rhodococcus (Rhodococcus) CGMCC No.3276, wherein the formula of the enlarging culture medium is as follows: 100g of sucrose, 7g of peptone, 7g of yeast extract and KH2PO41g，MgSO40.5, 1L of distilled water, and the pH value of the solution is 4.0-6.5.
The invention also provides a method for reducing aphid incidence, which comprises the step of spraying the mixed liquid of microzyme (Saccharomyces) CGMCC No.3278) and Rhodococcus (Rhodococcus) CGMCC No.3276 on the whole plant.
The method for reducing the aphid incidence rate, provided by the invention, is characterized in that the volume ratio of yeast (Saccharomyces) CGMCC No.3278 to Rhodococcus (Rhodococcus) CGMCC No.3276 is 2: 1.
The invention also provides application of the aphid repellent agent in reducing the incidence rate of plant aphids, wherein the plants can be crops, vegetables and fruit crops.
The invention also provides application of the aphid repellent agent in improving crop yield, wherein the crops are corn, wheat, soybean and/or rice.
The aphid repellent agent can obviously reduce the incidence rate of aphids, can promote the yield increase of crops, does not damage the environment composition, and has low application cost and obvious insect expelling effect.
Drawings
FIG. 1 shows the growth of wheat after spraying with the aphid repellents of the invention.
Detailed Description
Example 1 preparation of aphid repellents
The volume ratio of the yeast (Saccharomyces) CGMCC No.3278 to the Rhodococcus (Rhodococcus) CGMCC No.3276 is 2: 1. The greasiness-expelling biological microbial inoculum is produced by using an amplification culture medium for production and amplification culture, and the formula of the amplification culture medium is as follows: 100g of sucrose, 7g of peptone, 7g of yeast extract and KH2PO41g，MgSO40.5, 1L of distilled water, and the pH value of the solution is 4.0-6.5.
And diluting the expanded culture by 100 times, stirring uniformly, transferring into a sprayer, and directly spraying the whole plant. The number of spraying is determined according to the growth characteristics of the plants, for example, wheat is sprayed for 2-3 times.
Controls without any chemical and biological pesticide applied were set, controls with only chemical pesticide applied, test group with CGMCC No.3278 applied, test group with CGMCC No.3276 applied, test group with yeast (Saccharomyces) CGMCC No.3278 and Rhodococcus (Rhodococcus) CGMCC No.3276 applied.
Test results show that the aphid repellent agent reduces the incidence rate of aphids by more than 90 percent and increases the yield by 10 to 20 percent. The pesticide reduces the incidence of aphids by about 60-70%.
The test result shows that the test group applying the microzyme (Saccharomyces) CGMCC No.3278 and the Rhodococcus (Rhodococcus) CGMCC No.3276 has the synergistic technical effect on reducing the aphid incidence.
In 2017, 4 months, 10 days to 5 months, 20 days, the aphid repellent agent is diluted by 100 times, the whole plant is sprayed for 2 times in total, the yield per mu is 1300 jin, and 300 jin is produced per mu. Meanwhile, the cypermethrin pesticide is applied to the wheat outside 8 mu of test field for 3 times, 500 ml of the cypermethrin pesticide is directly sprayed to the whole wheat field each time, and the yield per mu is 1000 jin.
In 2017, in 5-8 months, a pear bagging test is carried out in a pear producing area in Zhao county, Hebei, but the aphid repellent agent is diluted by 100 times in advance before the coming value of aphids, the whole plant is sprayed, the spraying is carried out for 4 times in total, the yield per mu is 2800 jin, and 840 jin is produced per mu. And the yield per mu of the bagged pears is 1960 jin at the same time.
Claims (1)
1. A method for reducing aphid incidence is characterized in that yeast (Saccharomycetes) is sprayed on the whole plantSaccharomyces) CGMCC No.3278 and Rhodococcus (Rhodococcus) CGMCC No.3276 mixed liquid, wherein the yeast (Saccharomycetes cerevisiae) (yeast)Saccharomyces) CGMCC No.3278 and Rhodococcus (Rhodococcus) The volume ratio of CGMCC No.3276 is 2: 1.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810562560 | 2018-06-04 | ||
| CN2018105625604 | 2018-06-04 |
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| CN109730093A CN109730093A (en) | 2019-05-10 |
| CN109730093B true CN109730093B (en) | 2021-07-20 |
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| CN201811629756.7A Active CN109730093B (en) | 2018-06-04 | 2018-12-29 | Aphid repellent agent and preparation method and application thereof |
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| DE102006020728A1 (en) * | 2005-04-27 | 2006-11-09 | Technische Universität Dresden | Coating material, useful for coating a surface, comprises bio-emulsifying agent or a mixture of bio emulsifying agent |
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