CN1395839A - Preparing process and usage of muscardine insecticide - Google Patents
Preparing process and usage of muscardine insecticide Download PDFInfo
- Publication number
- CN1395839A CN1395839A CN 02116782 CN02116782A CN1395839A CN 1395839 A CN1395839 A CN 1395839A CN 02116782 CN02116782 CN 02116782 CN 02116782 A CN02116782 A CN 02116782A CN 1395839 A CN1395839 A CN 1395839A
- Authority
- CN
- China
- Prior art keywords
- culture
- tank
- jar
- sterilization
- muscardine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A muscardine insecticide for eliminating grub is prepared through collecting the dead sick grubs, separating, purifying, screening to obtain the muscardine D5 strain, and culturing in liquid to obtain the product. Its advantages are high effect and short preparing period.
Description
The present invention relates to the white muscardine fungi that can be used as biological insecticides that a kind of separation screening obtains, (a kind of muscardine of the muscardine in the white muscardine fungi particularly, be kept at China Committee for Culture Collection of Microorganisms common micro-organisms center on April 17th, 2002, it abbreviates CGMCC as, and deposit number is CGMCC NO.0737) seed selection, production method and in the application in biological insecticides field.
White muscardine fungi can kill off the insect pests by the body wall contact infection in natural conditions, evidence, kind of insect surplus the white muscardine fungi energy parasitic 700,, insect safer to people and animals and environment generally is difficult for developing immunity to drugs, can uses simultaneously with some chemical pesticide (insecticide, miticide, bactericide).
China white muscardine fungi began to produce in batches and use from the fifties, great majority adopt to produce by indigenous method promptly uses triangle shake-flask culture liquid spawn, insert in the solid culture medium and cultivate, long (solid culture 7～10 days of this explained hereafter cycle, dry 7 days), pollute easily, and yearly productive capacity has only 10～15 tons of former powder/factories.But the storage stability of product does not have solution, and general storage at normal temperature is in half a year.
At present, various crop such as grub harm peanut, soybean, lawn, vegetables, nursery, hazard area reaches several hundred million mus, only be example with the peanut, 4,500 ten thousand mu of peanuts are planted by China, endangered by grub, calculate with the white muscardine fungi control by 20% area, need 9,000,000 mu of controls, need 4500 tons in white muscardine fungi preparation.
The objective of the invention is to overcome the deficiency of prior art, seed selection is a kind of to have the desinsection white muscardine fungi (be grub special-purpose beauvericin insecticide) of fine killing ability to grub, designed with short production cycle, be suitable for suitability for industrialized production liquid processes production method and corresponding insecticidal preparation.
The present invention is achieved through the following technical solutions:
Feature of the present invention comprises muscardine (Beauveria bringniartii) CGMCC NO.0737 bacterial strain, production method, preparation and the application thereof of white muscardine fungi.
The bacterial strain that the present invention relates to is to gather the worm corpse of falling ill from the serious area of grub harm, the separation and purification row filter of going forward side by side, obtain muscardine (Beauveria bringniartii) D5 bacterial strain, be kept at China Committee for Culture Collection of Microorganisms common micro-organisms center on April 17th, 2002, it abbreviates CGMCC as, and deposit number is CGMCC NO.0737.This bacterial strain bacterium colony powdery, white, after become faint yellow.Conidiogenous cell Dan Sheng seldom clusters, and it is very thin to produce the spore axle, geniculation, and the little tooth of tool is prominent.Conidium is transparent, and is smooth, and ellipse, base portion have little point prominent sometimes, 2～2.5 μ m * 4.5～6 μ m.The highest to big black gill cockchafer larva virulence, reach 93.1%, sporulation quantity has improved 2.8 times than starting strain.
Muscardine D5 of the present invention (CGMCC NO.0737) the bacterial strain technological process of production is seen Figure of description 1.Production method of the present invention is described in detail as follows: (1) is with a kind of muscardine of the original production bacterial strain of D5, be kept at China Committee for Culture Collection of Microorganisms common micro-organisms center on April 17th, 2002, it abbreviates CGMCC as, deposit number is CGMCC NO.0737, evenly insert (production strain inclined plane culture medium prescription: sucrose 2% on the slant medium, dusty yeast 0.5%, peptone (fish) 0.5%, copper sulphate 0.005%, zinc sulphate 0.005%, ferrous sulfate 0.005%, cotton benevolence powder 0.5%, ground rice 0.5%, boric acid 0.01%, manganese sulphate 0.002%, lactose 0.3%, agar 2%), put and in 26 ℃ ± 1 ℃ incubator, cultivate 7～10d, take out when generation of conidium is good, can do to produce and use slant strains.(2) adding liquid nutrient medium or aqua sterilisa in the slant strains are washed culture and become bacteria suspension.(3) shake the bottle and seeding tank in liquid culture method with amount (the liquid culture based formulas: sucrose 2% of seed amplification culture to the required seed liquor of fermentation tank culture, dusty yeast 0.5%, peptone (fish) 0.5%, copper sulphate 0.005%, cotton benevolence powder 0.5%, ground rice 1%, magnesium sulfate 0.1%, ferrous sulfate 0.005%, potassium dihydrogen phosphate 0.2%.), canned coefficient: 70%.Fermentating controling condition: tank pressure 0.2kg/cm
2, throughput is 1: 1 (v/v/min), 27 ℃ ± 1 ℃ of jar temperature, and incubation time is 36h, and the mycelia amount is big, and the retrogradation of bacterium liquid has a small amount of blastopore to form, and is pollution-free, can be used as seed liquor.(4) sterilize and the pipeline sterilization for real jar: when fermentation tank carries out reality jar sterilization, carry out the sterilization of seeding tank and fermentation tank inoculation pipeline, each relevant drain tap on the crack pipeline must make the whole piece pipeline unimpeded during sterilization, do not become the dead angle, regulate intake valve to managing internal pressure 1.2kg/cm
2, pressurize 30min closes each intake valve successively from A-P then, cools off stand-by.Poor by two tank pressures, qualified secondary liquid kind is pressed onto fermentation tank in 1: 10 ratio, press kind to finish and closes two jars of bleeder valves rapidly, regulate pressure tank and inlet and outlet valve then, start stirring, cultivate.Cultivate: mixing speed 200r/min, 27 ℃ ± 1 ℃ of jar temperature, jar internal pressure 0.5kg/cm
2, throughput 1: 0.7 (v/v/min). and cultivate 48～72h.Ferment when 24～36h forms mycelium in a large number, replenish glucose 1%, lactose 0.3%, boric acid 0.02%, potassium dihydrogen phosphate 0.3%, glycerine 4%), in cultivation every the 4h sampling check once.Put jar when (5) mycelia all is converted into blastopore more than 80% during the big volume production spore of zymotic fluid.Add solid packing copper sulphate and auxiliary agent wheat bran during fermentation ends through sterilization treatment.Water content 20%～30% behind interpolation auxiliary agent and the filler.(6) the dry processing: 35～38 ℃ of dryings 2～3 days.Preparation: granule 20 order corn granules (particle that corn flour sieves) sterilization, soak mixedly, dry with the copper sulphate of carrier amount 0.2%, 0.2% copper sulphate bacteria suspension is adsorbed on the carrier as carrier, dry water content to 4%～5%, spore is produced in the recovery of measuring in the soil.Dust granule: use wheat bran as carrier.Fine granule: (50% corn flour, 50% attapulgite be as carrier, and makes particulate behind the bacterium liquid mixing 1. to use concavo-convex rod nutrition soil.
2. use concavo-convex rod nutrition soil (25% analysis for soybean powder, 25% corn flour; 50% attapulgite is as carrier, and makes particulate behind the bacterium liquid mixing.The dressing pulvis: 30% flour, 20% corn flour, 50% attapulgite with dry behind the bacterium liquid mixing, is checked coating effect, recovers to produce spore effect and spore dispersion effect.Product quality inspection 1 target level of product quality 1.1 spore contents: 10 * 10
8/ g.The spore rate 1.2 live: more than 90%.1.3 moisture content: 4%～5%.1.4 particle size: cross 36 mesh sieves.1.5 spore virulence: carry out biologicall test with big black grub at the beginning of 2 ages.Get larva and insert the bottom of plastic casing (Φ=4.5 centimetre, high 5.0 centimetres), on cover 80 gram water content 7%, spore content 0.1 * 10
8Wet sand, adding a cover the back is 25 ℃, RH 75% constant temperature indoor cultivation 15d in temperature.10d, grub occurs dead, and 15d grub lethality reaches more than 80%.2 methods of inspection, 2.1 sample extraction: every batch of product extracts a sample, and the one thousandth that is equivalent to the product sum is made submitted sample, shakeouts by the diagonal method of average or random sampling point-like after sample is stirred and extracts every check small sample.2.2 spore content measures 10 * 10
8/ g: precision takes by weighing is examined preparation 3g, pours in the glass jar of tissue mashing machine, adds 0.1% Tween-80 water 300ml, adds in the glass jar, smashs 1min to pieces; Make spore suspension, cover the cover glass of supporting special use with blood counting chamber (16 * 25 or 25 * 16 tallies), draw bacterium liquid with dropper or suction pipe, splash into along the cover glass edge, make bacterium liquid be full of gap (bubble can not be arranged, in the lateral sulcus spillage can not be arranged) between cover glass and the slide, static 30 seconds, with the sediments microscope inspection counting, each sample is established three repetitions, gets its mean value.
It is 25 middle lattice that blood cell calculates plate counting lattice, and lattice are 16 little lattice in each, and the total spore in totally 80 little lattice is in number then to calculate in the two-wire scope four middle lattice on four angles and middle middle lattice, and calculating formula is:
The spore rate of living is measured: after 500ml triangular flask or the cooling of 250ml nutrient solution (peptone 0.5%, sucrose 2%) autoclaving, put bacterium powder 0.1g to be measured, place constant temperature on the 140r/min shaking table (26 ℃ ± 1 ℃) to cultivate 24h, sampling film-making microscopy, get 6 visuals field, number goes out each visual field germinate (the former spore of spore expansion ratio big 1 times or bud length greater than spore radius) and germinating spore number not, calculates spore in the spore rate of living by following formula.
2.4 measurement of water-content coefficient: earlier the glass ware is dried in 120 ℃ of drying boxes, take out behind the 15min and weigh, claim 5g bacterium powder again in ware, constant temperature baking 2h in 120 ℃ of drying boxes, take out immediately with sample and glass ware, weigh together, each sample is done three repetitions, get its mean value, calculate moisture content by following formula.2.5 particle size is measured: take by weighing each 500g of pulvis to be measured and conidial powder, pulvis is all by 36 mesh sieves, and it is qualified to be.2.6 bacterium powder toxicity test: indoorly do biologicall test, get at the beginning of 2 ages 50 of healthy grubs, insert the bottom of plastic casing (Φ=4.5cm, high 5.0cm) respectively, on cover 80 gram water content 7%, spore content 0.1 * 10
8Wet sand, adding a cover the back is 25 ℃, the constant temperature indoor cultivation 15d of RH 75% in temperature, the dead borer population of observed and recorded grub day by day, each sample is done three repetitions, establishes blank simultaneously, by formula calculates lethality and the corrected mortality of grub.
The present invention relates to storage, transportation and the points for attention of goods
Storage should be selected drying, ventilation, lucifuge place.Storage at normal temperature is no more than half a year, surpasses must being put in 4 ℃ and locating refrigeration of using half a year.
Should moistureproof, sun-proof, anti-high temperature (being lower than 35 ℃) in the transportation.Forbid using with bactericide.
The present invention gathers the worm corpse of falling ill from the serious area of grub harm, and the separation and purification row filter of going forward side by side obtains muscardine D5 (CGMCC NO.0737) bacterial strain, and is the highest to big black gill cockchafer larva virulence, reaches 93.1%, and sporulation quantity has improved 2.8 times than starting strain.Fermentation period has only 48～72h, overcome now to be used to produce long shortcoming of beauvericin insecticide production cycle, and production method of the present invention has the characteristics of industrial-scale production.Have is exactly that pesticide product involved in the present invention has the specificity that grub is killed again.
Fig. 1 muscardine D5 (CGMCC NO.0737) bacterial strain technological process of production
Embodiment 11 produces bacterial classification 1.1 and produces the strain inclined plane culture medium prescription: sucrose 2%, dusty yeast 0.5%, peptone (fish) 0.5%, copper sulphate 0.005%, zinc sulphate 0.005%, ferrous sulfate 0.005%, cotton benevolence powder 0.5%, ground rice 0.5%, boric acid 0.01%, manganese sulphate 0.002%, lactose 0.3%, agar 2%.By being prepared into slant medium after every bottle of (200ml) loading amount 60ml packing, after 37 ℃ of cultivation 24h steriling tests are qualified, can use.2.1.2 condition of culture and time: behind the above-mentioned production bacterium culture medium of muscardine D5 (CGMCC NO.0737) the aseptic access of strain culture, in 26 ℃ ± 1 ℃ incubator, cultivate 7～10d, take out when generation of conidium is good, can make bacterial classification and use.2.1.3 storage requirement: this inoculum is cultivated rearmounted 4～10 ℃ in the access node bundle, uses in 30 days.2 shake bottle bacterial classification 2.1 culture medium prescriptions: sucrose 2%, dusty yeast 0.5%, peptone (fish) 0.5%, copper sulphate 0.005%, cotton benevolence powder 0.5%, ground rice 1%, magnesium sulfate 0.1%, ferrous sulfate 0.005%, potassium dihydrogen phosphate 0.2%.121 ℃ of sterilising temps, pressure 1.0kg/cm
2, pressurize 30min.PH control: it is preceding 6.2 to disappear, and back 6.0 disappears.2.2 inoculate the back in rotating speed 200r/min, 27 ± 1 ℃ of temperature, incubation time 36h, the mycelia wall built-up, pollution-free, can do bacterial classification and use.3 level liquid seed fermentations, 3.1 culture medium prescriptions: sucrose 2%, dusty yeast 0.5%, peptone (fish) 0.5%, copper sulphate 0.005%, cotton benevolence powder 0.5%, ground rice 1%, magnesium sulfate 0.1%, ferrous sulfate 0.005%, potassium dihydrogen phosphate 0.2%.121 ℃ of sterilising temps, pressure 1.0kg/cm
2, pressurize 30min.PH control: it is preceding 6.2 to disappear, and back 6.0 disappears.Canned coefficient: 70%.When treating that medium cools to 30 ℃, the grain weight by 3% inserts seed.3.2 fermentating controling condition: tank pressure 0.2kg/cm
2, throughput is 1: 1 (v/v/min), 27 ℃ ± 1 ℃ of jar temperature, and incubation time is 36h, and the mycelia amount is big, and the retrogradation of bacterium liquid has a small amount of blastopore to form, and is pollution-free, can be used as secondary seed.3.3 sampling detects: 12h begins after inoculation, every 4h sampling carrying out microscopy detects the mycelia form, blastopore quantity, pH value, the living contaminants of denying etc. is arranged.4 secondary liquid seeds, 4.1 culture medium prescriptions that ferment: sucrose 2%, dusty yeast 0.5%, peptone (fish) 0.5%, copper sulphate 0.005%, cotton benevolence powder 0.5%, ground rice 1%, magnesium sulfate 0.1%, ferrous sulfate 0.005%, potassium dihydrogen phosphate 0.2%.121 ℃ of sterilising temps, pressure 1.0kg/cm
2, pressurize 30min.PH control: it is preceding 6.2 to disappear, and back 6.0 disappears.Canned coefficient: 70%.When treating that medium cools to 30 ℃, insert seed by 10% grain weight.4.2 fermentating controling condition: tank pressure 0.2kg/cm
2, throughput is 1: 1 (v/v/min), 27 ℃ ± 1 ℃ of jar temperature, and incubation time is 30-36h, and the mycelia amount is big, and the retrogradation of bacterium liquid has a small amount of blastopore to form, and is pollution-free, can be used as secondary seed.4.3 sampling detects: 12h begins after inoculation, every 4h sampling carrying out microscopy detects the mycelia form, blastopore quantity, pH value, the living contaminants of denying etc. is arranged.5 ferment tanks, 5.1 culture medium prescriptions: sucrose 2%, dusty yeast 0.5%, peptone (fish) 0.5%, copper sulphate 0.005%, cotton benevolence powder 0.5%, ground rice 1%, magnesium sulfate 0.1%, ferrous sulfate 0.005%, potassium dihydrogen phosphate 0.2%.5.2 tank body sterilization: pressure 1.2kg/cm
2, pressurize 30min.5.3 sterilize and the pipeline sterilization for real jar: when fermentation tank carries out reality jar sterilization, carry out the sterilization of seeding tank and fermentation tank inoculation pipeline, each relevant drain tap on the crack pipeline must make the whole piece pipeline unimpeded during sterilization, do not become the dead angle, regulate intake valve to managing internal pressure 1.2kg/cm
2, pressurize 30min closes each intake valve successively from A-P then, cools off stand-by.5.4 culture transferring: poor by two tank pressures, qualified secondary liquid kind is pressed onto fermentation tank in 1: 10 ratio, press kind to finish and closes two jars of bleeder valves rapidly, regulate pressure tank and inlet and outlet valve then, start stirring, cultivate.5.5 cultivate: mixing speed 200r/min, 27 ℃ ± 1 ℃ of jar temperature, jar internal pressure 0.5kg/cm
2, throughput 1: 0.7 (v/v/min).Cultivate 48～72h.Ferment when 24～36h forms blastopore in a large number, replenish glucose 1%, lactose 0.3%, boric acid 0.02%, potassium dihydrogen phosphate 0.3%, glycerine 4%), in cultivation every the 4h sampling check once.5.6 liquid kind jar fermentation management: once jar temperature, throughput, tank pressure of registration per hour, 12h begins after inoculation, and every 4h sampling carrying out microscopy is measured the mycelia form, blastopore quantity, pH value, the living contaminants of denying etc. is arranged.(solid additive is a copper sulphate etc. through the solid packing of sterilization treatment and auxiliary agent 5.7 add during (put when mycelia all is converted into blastopore more than 80% jar) fermentation ends during the big volume production spore of zymotic fluid; Filler is a wheat bran)., water content 20%～30% behind interpolation auxiliary agent and the filler.5.8 dry processing the: 35～38 ℃ of dryings 2～3 days.6 preparations: granule corn granule (particle that corn flour sieves) sterilization, soak mixedly, dry with the copper sulphate of carrier amount 0.2%, 0.2% copper sulphate bacteria suspension is adsorbed on the carrier as carrier, dry water content to 4%～5%, spore is produced in the recovery of measuring in the soil.Dust granule: use wheat bran as carrier.Granule: 20 order iblets are made carrier.Fine granule: (50% corn flour, 50% attapulgite be as carrier, and makes particulate behind the bacterium liquid mixing 1. to use concavo-convex rod nutrition soil.
2. use concavo-convex rod nutrition soil (25% analysis for soybean powder, 25% corn flour; 50% attapulgite is as carrier, and makes particulate behind the bacterium liquid mixing.The dressing pulvis: 30% flour, 20% corn flour, 50% attapulgite with dry behind the bacterium liquid mixing, is checked coating effect, recovers to produce spore effect and spore dispersion effect.7 products are stored
Product drying is to water content 4%～6%.Storage at normal temperature behind the oxygen barrier film packaging seal.The control efficiency of 1 pair of peanut grub of experiment is executed the bacterium method and is divided into: execute bacterium sowing time: bacterium powder that muscardine D5 (CGMCC NO.0737) bacterial strain is made with the present invention and quantitative native mixing apply in the soil pit together with seed during peanut seeding.The intertillage phase is executed bacterium: bacterium powder of muscardine D5 (CGMCC NO.0737) bacterial strain being made with the present invention in the intertillage phase and quantitative native mixing (bacterium local method), evenly spread near the peanut rhizosphere, again the bacterium powder is hugged bury in or the bacterium powder mixed in water, sprinkle to peanut root (water sprinkles method).The pond plants test and sow peanut in 1 cement pit of the no end, after emerging unnecessary seedling is pulled out, and the seedling number is equated.Execute bacterium in accordance with regulations in dosage and period.Treat at the beginning of grub to 2 age, by 5/m
2Worm amount insert in the pond investigation after autumn live borer population and the killed fruit number of peanut.Difference is executed bacterium phase result and is shown, intertillage phase control efficiency is better than sowing time, and when executing the bacterium metering for per hectare 75,000,000,000,000 spores, executing bacterium white grub (grub adult) larval mortality in the intertillage phase is 93.3%, and execute bacterium white grub larval mortality sowing time is 66.6%, significant difference.Difference is executed microbial inoculum amount result and is shown, executes bacterium in sowing time, executes the microbial inoculum amount and needs 150,000,000,000,000 spores/hm
2Desirable control efficiency is just arranged when above, and execute bacterium 75,000,000,000,000 spores/hm in the intertillage phase
2Bacterium medicine (+1.2kg Isofenphos methyl) is used with or list all can reach desirable control efficiency (the wormed fruit rate of going down can reach 90%) with bacterium.Field trial selects grub insect population radix consistent, endanger medium plot, and the employing difference executes bacterium period, dosage, execute the bacterium mode prevents and treats test.The result shows: intertillage phase control efficiency is better than sowing time, and to plant result of the test consistent with the pond.Execute in the intertillage phase and can obtain 80% control efficiency when the microbial inoculum amount is 150,000,000,000,000 spores/hm2 or 75,000,000,000,000 spores+1.2kg Isofenphos methyl/hm2.The bacterium medicine is used the consumption that can reduce bacterium with, improves control efficiency.In the intertillage phase, it is all more satisfactory to use two kinds of the bold and vigorous methods of bacterium local method and water to execute bacterium mode control efficiency, no significant difference.
Claims (3)
1. the production method of the special-purpose beauvericin insecticide of a grub, its feature mainly comprises following step: (1) is with the original production bacterial strain of D5, a kind of muscardine-Beauveria bringniartii, be kept at China Committee for Culture Collection of Microorganisms common micro-organisms center on April 17th, 2002, it abbreviates CGMCC as, deposit number is CGMCCNO.0737, evenly insert on the slant medium, put and in 26 ℃ ± 1 ℃ incubator, cultivate 7～10d, take out when generation of conidium is good, can do to produce and use slant strains.(2) adding liquid nutrient medium or aqua sterilisa in the slant strains are washed culture and become bacteria suspension.(3) shake the bottle and seeding tank in liquid culture method with the amount of seed amplification culture to the required seed liquor of fermentation tank culture, canned coefficient: 70%.Fermentating controling condition: tank pressure 0.2kg/cm
2, throughput is counted 1: 1 by v/v/min, 27 ℃ ± 1 ℃ of jar temperature, and incubation time is 36h, and the mycelia amount is big, and the retrogradation of bacterium liquid has a small amount of blastopore to form, and is pollution-free, can be used as seed liquor.(4) sterilize and the pipeline sterilization for real jar: when fermentation tank carries out reality jar sterilization, carry out the sterilization of seeding tank and fermentation tank inoculation pipeline, each relevant drain tap on the crack pipeline must make the whole piece pipeline unimpeded during sterilization, do not become the dead angle, regulate intake valve to managing internal pressure 1.2kg/cm
2, pressurize 30min closes each intake valve successively from A-P then, cools off stand-by.Poor by two tank pressures, qualified secondary liquid kind is pressed onto fermentation tank in 1: 10 ratio, press kind to finish and closes two jars of bleeder valves rapidly, regulate pressure tank and inlet and outlet valve then, start stirring, cultivate.Cultivate: mixing speed 200r/min, 27 ℃ ± 1 ℃ of jar temperature, jar internal pressure 0.5kg/cm
2, throughput is counted 1: 0.7 by v/v/min.Cultivate 48～72h.Ferment when 24～36h forms blastopore in a large number, replenish glucose 1%, lactose 0.3%, boric acid 0.02%, potassium dihydrogen phosphate 0.3%, glycerine 4%, in cultivation every the 4h sampling check once.Put jar when (5) mycelia all is converted into blastopore more than 80% during the big volume production spore of zymotic fluid.Add solid packing copper sulphate and auxiliary agent wheat bran during fermentation ends through sterilization treatment.Water content 20%～30% behind interpolation auxiliary agent and the filler.(6) produce the strain inclined plane culture medium prescription: sucrose 2%, dusty yeast 0.5%, fish peptone 0.5%, copper sulphate 0.005%, zinc sulphate 0.005%, ferrous sulfate 0.005%, cotton benevolence powder 0.5%, ground rice 0.5%, boric acid 0.01%, manganese sulphate 0.002%, lactose 0.3%, agar 2%.(7) liquid culture based formulas: sucrose 2%, dusty yeast 0.5%, fish peptone 0.5%, copper sulphate 0.005%, cotton benevolence powder 0.5%, ground rice 1%, magnesium sulfate 0.1%, ferrous sulfate 0.005%, potassium dihydrogen phosphate 0.2%.
2. the production method of the special-purpose beauvericin insecticide of the described grub of claim 1, it is characterized by its production bacterial strain is that a kind of muscardine-Beauveria bringniartii D5 bacterial strain has been kept at China Committee for Culture Collection of Microorganisms common micro-organisms center on April 17th, 2002, it abbreviates CGMCC as, and deposit number is CGMCC NO.0737.
3. the production method of the special-purpose beauvericin insecticide of the described grub of claim 1 is characterized by described production method and also comprises fermentation after fermentation liquid is processed into granule, dust granule, fine granule and dressing pulvis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021167826A CN1164179C (en) | 2002-05-13 | 2002-05-13 | Preparing process and usage of muscardine insecticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021167826A CN1164179C (en) | 2002-05-13 | 2002-05-13 | Preparing process and usage of muscardine insecticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1395839A true CN1395839A (en) | 2003-02-12 |
| CN1164179C CN1164179C (en) | 2004-09-01 |
Family
ID=4744232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021167826A Expired - Fee Related CN1164179C (en) | 2002-05-13 | 2002-05-13 | Preparing process and usage of muscardine insecticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1164179C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100341413C (en) * | 2006-01-10 | 2007-10-10 | 山西大学 | Beauveria bassiana preparation used for preventing and controlling pine moth, its preparation method and use |
| CN103283687A (en) * | 2013-06-21 | 2013-09-11 | 夏西超 | Method for preparing inactivated grubs through beauveria bassiana |
| CN101785482B (en) * | 2009-12-18 | 2013-09-18 | 江苏耕耘化学有限公司 | Pasty biological pesticide composite and preparation method thereof |
| CN106417385A (en) * | 2016-09-22 | 2017-02-22 | 江西天祥通用航空股份有限公司 | Hypocrea virens granules and preparation method thereof |
| CN108378063A (en) * | 2018-05-18 | 2018-08-10 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of muscardine granule |
| CN109337822A (en) * | 2018-10-10 | 2019-02-15 | 中国农业科学院植物保护研究所 | Muscardine BRNS50206 and its application |
| CN110896964A (en) * | 2019-12-19 | 2020-03-24 | 河南农贝得农业科技有限公司 | Seed dressing agent without pesticide residue and preparation method thereof |
-
2002
- 2002-05-13 CN CNB021167826A patent/CN1164179C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100341413C (en) * | 2006-01-10 | 2007-10-10 | 山西大学 | Beauveria bassiana preparation used for preventing and controlling pine moth, its preparation method and use |
| CN101785482B (en) * | 2009-12-18 | 2013-09-18 | 江苏耕耘化学有限公司 | Pasty biological pesticide composite and preparation method thereof |
| CN103283687A (en) * | 2013-06-21 | 2013-09-11 | 夏西超 | Method for preparing inactivated grubs through beauveria bassiana |
| CN106417385A (en) * | 2016-09-22 | 2017-02-22 | 江西天祥通用航空股份有限公司 | Hypocrea virens granules and preparation method thereof |
| CN108378063A (en) * | 2018-05-18 | 2018-08-10 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of muscardine granule |
| CN109337822A (en) * | 2018-10-10 | 2019-02-15 | 中国农业科学院植物保护研究所 | Muscardine BRNS50206 and its application |
| CN109337822B (en) * | 2018-10-10 | 2020-05-22 | 中国农业科学院植物保护研究所 | Beauveria bassiana BRNS50206 and application thereof |
| CN110896964A (en) * | 2019-12-19 | 2020-03-24 | 河南农贝得农业科技有限公司 | Seed dressing agent without pesticide residue and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1164179C (en) | 2004-09-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN105296381B (en) | One bacillus subtilis CYY-25 and its application | |
| CN101967455B (en) | Bacillus amyloliquefaciens EA19 for controlling wheat root diseases and preparation thereof | |
| CN105316243B (en) | A kind of preparation method and applications of agricultural root-knot nematode compound biocontrol fungicide | |
| CN101485336A (en) | Trichoderma sp. preparation for preventing and treating soil-borne disease of crop | |
| CN110616156B (en) | Trichoderma harzianum solid state fermentation culture medium, trichoderma harzianum conidium, biological agent comprising conidium and application of biological agent | |
| CN107711891A (en) | A kind of method prevented and treated matrimony vine root rot and extremely set | |
| WO2023124450A1 (en) | Bacillus velezensis strain and use thereof | |
| CN104671853A (en) | Preparation method of compound photosynthetic bacterial fertilizer | |
| CN105062916A (en) | Application of bacillus coagulans in improving egg production of laying hen | |
| CN104788175B (en) | Trichoderma biologic grain agent and its preparation method and application | |
| CN109971656B (en) | Ginger endogenetic trichoderma viride and application thereof | |
| CN103724058B (en) | Microbial fermentation functional organic compound fertilizer and production method thereof | |
| CN101935627B (en) | Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same | |
| CN1164179C (en) | Preparing process and usage of muscardine insecticide | |
| CN100368528C (en) | Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation | |
| CN108277172A (en) | The preparation of Paenibacillus polymyxa GY40 and its wettable powder and purposes | |
| CN102533564B (en) | A kind of screening method of bio-control trichoderma in corn seedling stage root rot period | |
| CN108265009B (en) | Culture medium for culturing beauveria bassiana and culture method thereof | |
| CN113999778B (en) | Trichoderma atroviride microsclerotia and preparation method and application of preparation thereof | |
| CN115651846A (en) | Trichoderma fungicide, biological organic fertilizer, preparation method and application thereof | |
| CN110184197B (en) | Beauveria bassiana oil suspending agent | |
| CN110724643B (en) | Penicillium citrinum with disease prevention and growth promotion functions, microbial inoculum and application thereof | |
| CN105967910A (en) | Microbial organic fertilizer capable of preventing and treating bacterial wilt as well as preparation method and application thereof | |
| CN107586748B (en) | A kind of China's sporangium and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20040901 Termination date: 20140513 |