CN101785482B - Pasty biological pesticide composite and preparation method thereof - Google Patents
Pasty biological pesticide composite and preparation method thereof Download PDFInfo
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- CN101785482B CN101785482B CN 200910264402 CN200910264402A CN101785482B CN 101785482 B CN101785482 B CN 101785482B CN 200910264402 CN200910264402 CN 200910264402 CN 200910264402 A CN200910264402 A CN 200910264402A CN 101785482 B CN101785482 B CN 101785482B
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Abstract
The invention discloses a pasty biological pesticide composite and a preparation method thereof. The biological pesticide composite comprises pesticide components and an auxiliary component. The pesticide components are biological prevention funguses. The biological pesticide composite is characterized in that the auxiliary material is starch which accounts for 5%-10% of the total weight of the composite. The biological prevention funguses refer to trichoderma, penicillium, chaetomium, cylindrocarpon, fusarium, Beauveria bassiana and/or metarhiziumanisopliae; a copper ion inhibitor, the addition amount of which is 0.001%-0.05% of the total weight of the composite, is added in the composite; in addition, hydrochloric acid, the addition amount of which is suitable for adjusting the pH valueto 3.0-4.0, is also added in the composite. The composite and biological pesticides prepared by using the method have the greatest advantages of stable product quality, long preservation time (18 months at normal temperature with high activity and 3 years in refrigeration condition); in addition, the invention has fastidious formula and advanced process, does not generate any wastewater, waste gas or waste residues, and is beneficial to environmental protection.
Description
Technical field
The present invention relates to a kind of biopesticide and preparation method thereof, especially a kind of biological pesticide composite of paste dosage-form, the present invention also will provide the preparation method of this paste dosage-form biological pesticide composite.
Background technology
At present the formulation of biopesticide is pulvis, wetting powder, suspending agent, missible oil, aqua, ovum card, the worm etc. that lives mostly, although have certain control efficiency, holding time all to fall short of to disease pest and weed etc., the quality of the pharmaceutical preparations is also stable not.
Summary of the invention
The purpose of this invention is to provide biological pesticide composite of a kind of paste dosage-form and preparation method thereof, the biological pesticide composite of this paste dosage-form can be good at preventing and treating plurality of plant diseases, the advantage of the paste dosage-form biopesticide maximum that is prepared into this method is constant product quality, holding time is long, can store at normal temperatures 18 months and can keep its high activity, under refrigerated condition, can store 3 years; Next the present invention fills a prescription fastidious, and technique is advanced, does not produce any waste water and gas waste residue, is beneficial to environmental protection, meets national pesticide producing policy.
The scheme of finishing the foregoing invention task is: a kind of biological pesticide composite of paste dosage-form, formed by pesticide composition and auxiliary material, and described pesticide composition is biocontrol fungi, it is characterized in that, described auxiliary material is starch; Described starch accounts for 5%～10% of composition total weight.
Biocontrol fungi described in the above scheme mainly refers to: Trichoderma, and Penicillium notatum, cupreum, post spore bacterium, sickle-like bacteria, white muscardine fungi, and/or green muscardine fungus etc.
The present invention has following prioritization scheme:
1, also be added with copper ion inhibitor in the composition, the addition of this copper ion inhibitor is the 0.001%-0.05% of composition total weight;
2, also be added with hydrochloric acid in the composition, the addition of described hydrochloric acid is as the criterion with the mould chlamydosporic pH to 3.0 of wood～4.0 to regulate mycelia;
3, described starch adopts potato starch.
The scheme of finishing the 2nd invention task of the application is: the preparation method of the biological pesticide composite of above-mentioned paste dosage-form, it is characterized in that, and step is as follows:
(1), the bacterial classification of biocontrol fungi is inoculated in the aseptic liquid nutrient medium by 2%～10% inoculum concentration, at 25～28 ℃, shake training 24～60h;
Wherein said Trichoderma is inoculated in the aseptic liquid nutrient medium by 2%～10% inoculum concentration, at 26～28 ℃, shakes training 36～48h under the rotating speed of 150rpm/min;
Described Penicillium notatum is inoculated in the aseptic liquid nutrient medium by 2%～10% inoculum concentration, at 25～28 ℃, shakes training 48～60h under the rotating speed of 150rpm/min;
Described cupreum is inoculated in the aseptic liquid nutrient medium by 5%～10% inoculum concentration, at 26～28 ℃, shakes training 48～60h under the rotating speed of 150rpm/min;
Described post spore bacterium is inoculated in the aseptic liquid nutrient medium by 5%～10% inoculum concentration, at 26～28 ℃, shakes training 48～60h under the rotating speed of 150rpm/min;
Described sickle-like bacteria is inoculated in the aseptic liquid nutrient medium by 2%～5% inoculum concentration, at 25～27 ℃, shakes training 36～48h under the rotating speed of 150rpm/min;
Described white muscardine fungi is inoculated in the aseptic liquid nutrient medium by 5%～10% inoculum concentration, at 26～28 ℃, shakes training 48～60h under the rotating speed of 150rpm/min;
Described green muscardine fungus is inoculated in the aseptic liquid nutrient medium by 5%～10% inoculum concentration, at 26～28 ℃, shakes training 48～60h under the rotating speed of 150rpm/min;
(2), wait shake training good after, filter, the mycelia after filtering is taken out;
(3), regulate mycelia PH to 3.0～4.0 with hydrochloric acid;
(4) and add the copper ion inhibitor of 0.001%-0.05%;
(5), again add 5%～10% starch, stir and namely made the biopesticide of paste dosage-form.
The present invention recommends: described starch adopts potato starch.
Above preparation process needs to operate under aseptic condition.
The advantage of composition of the present invention and the paste dosage-form biopesticide maximum that is prepared into this method is constant product quality, and the holding time is long, can store at normal temperatures 18 months and can keep its high activity, can store under refrigerated condition 3 years; And the wooden mould pulvis that prior art makes and white muscardine fungi oil suspending agent storage life are 1 year, and the Paecilomyces lilacinus microbial inoculum can not long preservation (documents " preparation technology of trichoderma as biological agricultural chemicals ", " a kind of white muscardine fungi oil suspending agent and preparation method thereof ", " producing the method for Paecilomyces lilacinus microbial inoculum with brewery's waste liquid "); Next the present invention fills a prescription fastidious, and technique is advanced, does not produce any waste water and gas waste residue, is beneficial to environmental protection, meets national pesticide producing policy.
Compare with the biocontrol fungi ointment preparation that just has been prepared into, store and to keep its highly active assay method as follows after 18 months: with Fusarium spp., Alternaria alternata, Botrytiscinerea, Aspergillus niger, Rhizopus nigricans, Aspergillus versicolor, 7 kinds of pathogens such as Penicilliumvariable are indicator bacteria, get respectively biocontrol fungi ointment preparation and the biocontrol fungi ointment preparation of storage after 18 months that equivalent just has been prepared into, inoculum concentration by 2% is inoculated in the liquid nutrient medium 26 ℃, after shaking training 48h under the rotating speed of 150rpm/min, inoculate respectively 100ul and shake training liquid to the Cha Shi plating medium, after bacterium colony covers with flat board behind 5～7d, adopt the card punch of 5mm after the colony edge punching; Same cultured indicator bacteria also adopts the card punch of 5mm to punch at colony edge, adopt dull and stereotyped face-off cultivation (plate diameter 9cm), biocontrol fungi dish and indicator bacteria bacterium dish are placed same PDA (glucose agar medium) flat board, two bacterium dish and dull and stereotyped center are linearly, and the distance at two bacterium dish and dull and stereotyped center is 2.25cm, measure Trichoderma after placing 26 constant incubators to cultivate 7d the flat board for preparing, Penicillium notatum, cupreum, post spore bacterium, sickle-like bacteria, white muscardine fungi, and/or green muscardine fungus is to the inhibiting rate of each indicator bacteria.The result is as follows:
Table 1: just prepared with store 18 after wooden mould ointment preparation to the inhibiting rate (%) of Various Diseases fungal pathogens
Table 2: just prepared with store 18 after the mould ointment preparation to the inhibiting rate (%) of Various Diseases fungal pathogens
Table 3: just prepared with store 18 after the cupreum ointment preparation to the inhibiting rate (%) of Various Diseases fungal pathogens
Table 4: just prepared with store 18 after post spore bacterium ointment preparation to the inhibiting rate (%) of Various Diseases fungal pathogens
Table 5: just prepared with store 18 after the sickle-like bacteria ointment preparation to the inhibiting rate (%) of Various Diseases fungal pathogens
Table 6: just prepared with store 18 after the white muscardine fungi ointment preparation to the inhibiting rate (%) of Various Diseases fungal pathogens
Table 7: just prepared with store 18 after the green muscardine fungus ointment preparation to the inhibiting rate (%) of Various Diseases fungal pathogens
From table 1, we can find out that the biocontrol fungi ointment preparation that stores after 18 months and the inhibiting rate of the ointment preparation that has just prepared to various disease funguses are more or less the same, and illustrate that the biocontrol fungi ointment preparation after preserving can keep its high activity.
Description of drawings
Embodiment
Embodiment 1, wooden mould liquid culture based formulas: beet liquid 0～20.0g, (NH
4)
2SO
40～2.0g, MgSO
47H
2O 0～0.2g, KH
2PO
40～2.0g, sucrose 0～10.0g;
Biological pesticide composite of paste dosage-form and preparation method thereof: composition of the present invention has: Trichoderma silk, wooden mould chlamydospore, hydrochloric acid, potato starch and copper ion.
Adopt wooden mould liquid culture based formulas 1: beet liquid 20.0g, (NH
4)
2SO
40g, MgSO
47H
2O0.2g, KH
2PO
42.0g, sucrose 0g;
Mould by 5% inoculum concentration inoculation wood, at 26 ℃, shake training 36h under the 150rpm/min after, filter, the mycelia after filtering is taken out, regulate mycelia PH to 3.5 with hydrochloric acid, and add a small amount of copper ion inhibitor, and add again 10% potato starch, stir and namely made the biopesticide of paste dosage-form.This preparation process needs to operate under aseptic condition.
Embodiment 2, and is substantially the same manner as Example 1, but is inoculated in the aseptic liquid nutrient medium by 2% inoculum concentration; Shake training 48h at 28 ℃, after the mycelia after filtering is taken out, regulate mycelia pH to 3.0 with hydrochloric acid, add a small amount of copper ion inhibitor, add again 8% potato starch.
The mould liquid culture based formulas of wood is: beet liquid 20.0g, (NH
4)
2SO
40g, MgSO
47H
2O 0.2g, KH
2PO
42.0g, sucrose 0g.
Embodiment 3, and is substantially the same manner as Example 1, but is inoculated in the aseptic liquid nutrient medium by 10% inoculum concentration; At 27 ℃, shake training 40h, after the mycelia after filtering is taken out, regulate mycelia pH to 4.0 with hydrochloric acid, add a small amount of copper ion inhibitor, add again 5% potato starch.
The mould liquid culture based formulas of wood is: beet liquid 20.0g, (NH
4)
2SO
40g, MgSO
47H
2O 0.2g, KH
2PO
42.0g, sucrose 5.0g.
Embodiment 4, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 0～20.0g, (NH
4)
2SO
40～2.0g, MgSO
47H
2O 0～0.2g, KH
2PO
40～2.0g, sucrose 0～10.0g;
Embodiment 5, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
40g, MgSO
47H
2O 0.2g, KH
2PO
42.0g, sucrose 10.0g.
Embodiment 6, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
42.0g, MgSO
47H
2O 0.2g, KH
2PO
42.0g, sucrose 0g.
Embodiment 7, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
42.0g, MgSO
47H
2O 0.2g, KH
2PO
42.0g, sucrose 5.0g.
Embodiment 8, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
42.0g, MgSO
47H
2O 0.2g, KH
2PO
42.0g, sucrose 10.0g.
Embodiment 9, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
42.0g, MgSO
47H
2O 0g, KH
2PO
42.0g, sucrose 0g.
Embodiment 10, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
42.0g, MgSO
47H
2O 0g, KH
2PO
42.0g, sucrose 10.0g.
Embodiment 11, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
42.0g, MgSO
47H
2O 0.2g, KH
2PO
40g, sucrose 0g.
Embodiment 12, and is substantially the same manner as Example 1, but wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
42.0g, MgSO
47H
2O 0.2g, KH
2PO
40g, sucrose 10.0g.
Embodiment 13, and is substantially the same manner as Example 1, but described biocontrol fungi adopts Penicillium notatum, and liquid nutrient medium is potato 200g, glucose 20g.
Embodiment 14, and is substantially the same manner as Example 1, but described biocontrol fungi adopts cupreum, and liquid nutrient medium is potato 200g, glucose 20g.
Embodiment 15, and is substantially the same manner as Example 1, but described biocontrol fungi adopts post spore bacterium, and liquid nutrient medium is potato 200g, glucose 20g.
Embodiment 16, and is substantially the same manner as Example 1, but described biocontrol fungi adopts sickle-like bacteria, and liquid nutrient medium is potato 200g, glucose 20g.
Embodiment 17, and is substantially the same manner as Example 1, but described biocontrol fungi adopts white muscardine fungi, and liquid nutrient medium is potato 200g, glucose 20g.
Embodiment 18, and is substantially the same manner as Example 1, but described biocontrol fungi adopts green muscardine fungus, and liquid nutrient medium is potato 200g, glucose 20g.
Claims (7)
1. the biological pesticide composite of a paste dosage-form is comprised of pesticide composition and auxiliary material, and described pesticide composition is biocontrol fungi, it is characterized in that, described auxiliary material is starch; Described starch accounts for 5% ~ 10% of composition total weight;
Also be added with copper ion inhibitor in the said composition, the addition of this copper ion inhibitor is 0.001% ~ 0.05% of composition total weight;
Also be added with hydrochloric acid in the said composition, the addition of described hydrochloric acid is as the criterion to regulate pH to 3.0 ~ 4.0; Described biocontrol fungi refers to: Trichoderma, Penicillium notatum, cupreum, post spore bacterium, sickle-like bacteria, white muscardine fungi, and/or green muscardine fungus.
2. the biological pesticide composite of paste dosage-form according to claim 1 is characterized in that, described starch adopts potato starch.
3. the preparation method of the biological pesticide composite of paste dosage-form claimed in claim 1 is characterized in that, step is as follows:
⑴, the bacterial classification of biocontrol fungi is inoculated in the aseptic liquid nutrient medium by 2% ~ 10% inoculum concentration, at 25 ~ 28 ℃, shakes training 36 ~ 60h under the rotating speed of 150 rpm/min;
, wait shake training good after, filter, the mycelia after filtering is taken out;
⑶, regulate mycelia PH to 3.0 ~ 4.0 with hydrochloric acid;
⑷ also add the copper ion inhibitor of 0.001%-0.05%;
⑸, add 5% ~ 10% starch again, stir and namely made the biopesticide of paste dosage-form.
4. the preparation method of the biological pesticide composite of paste dosage-form claimed in claim 3 is characterized in that,
Wherein said Trichoderma is inoculated in the aseptic liquid nutrient medium by 2% ~ 10% inoculum concentration, at 26 ~ 28 ℃, shakes training 36 ~ 48h under the rotating speed of 150 rpm/min;
Described Penicillium notatum is inoculated in the aseptic liquid nutrient medium by 2% ~ 10% inoculum concentration, at 25 ~ 28 ℃, shakes training 48 ~ 60h under the rotating speed of 150 rpm/min;
Described cupreum is inoculated in the aseptic liquid nutrient medium by 5% ~ 10% inoculum concentration, at 26 ~ 28 ℃, shakes training 48 ~ 60h under the rotating speed of 150 rpm/min;
Described post spore bacterium is inoculated in the aseptic liquid nutrient medium by 5% ~ 10% inoculum concentration, at 26 ~ 28 ℃, shakes training 48 ~ 60h under the rotating speed of 150 rpm/min;
Described sickle-like bacteria is inoculated in the aseptic liquid nutrient medium by 2% ~ 5% inoculum concentration, at 25 ~ 27 ℃, shakes training 36 ~ 48h under the rotating speed of 150 rpm/min;
Described white muscardine fungi is inoculated in the aseptic liquid nutrient medium by 5% ~ 10% inoculum concentration, at 26 ~ 28 ℃, shakes training 48 ~ 60h under the rotating speed of 150 rpm/min;
Described green muscardine fungus is inoculated in the aseptic liquid nutrient medium by 5% ~ 10% inoculum concentration, at 26 ~ 28 ℃, shakes training 48 ~ 60h under the rotating speed of 150 rpm/min.
5. according to claim 3 or the preparation method of the biological pesticide composite of 4 described paste dosage-forms, it is characterized in that, described starch adopts potato starch.
6. the preparation method of the biological pesticide composite of paste dosage-form according to claim 5 is characterized in that, the liquid culture based formulas of biocontrol fungi is selected from one of following prescription:
The mould liquid culture based formulas of wood is: beet liquid 0 ~ 20.0g, (NH
4)
2SO
40 ~ 2.0g, MgSO
47H
2O 0 ~ 0.2g, KH
2PO
40 ~ 2.0g, sucrose 0 ~ 10.0g;
Penicillium notatum, cupreum, post spore bacterium, sickle-like bacteria, white muscardine fungi, and/or the liquid culture based formulas of green muscardine fungus is: potato 200g, glucose 20g.
7. the preparation method of the biological pesticide composite of paste dosage-form according to claim 6 is characterized in that, wooden mould liquid culture based formulas is: beet liquid 20.0g, (NH
4)
2SO
40g, MgSO
47H
2O 0.2g, KH
2PO
42.0g, sucrose 0g;
Its preparation process needs to operate under aseptic condition.
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|---|---|---|---|
| CN 200910264402 CN101785482B (en) | 2009-12-18 | 2009-12-18 | Pasty biological pesticide composite and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910264402 CN101785482B (en) | 2009-12-18 | 2009-12-18 | Pasty biological pesticide composite and preparation method thereof |
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|---|---|
| CN101785482A CN101785482A (en) | 2010-07-28 |
| CN101785482B true CN101785482B (en) | 2013-09-18 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217592A (en) * | 2011-04-26 | 2011-10-19 | 福建省林业科学研究院 | Fungus sticking paste and making method and application of the fungus sticking paste |
| CN103013838B (en) * | 2012-12-14 | 2015-05-13 | 江苏耕耘化学有限公司 | Pasty biocontrol preparation for strawberry greensickness, and preparation method, application and special strain thereof |
| CN104872217A (en) * | 2015-06-01 | 2015-09-02 | 吴迪 | Biopesticide for preventing cydia pomonella |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1395839A (en) * | 2002-05-13 | 2003-02-12 | 中国农业科学院生物防治研究所 | Preparing process and usage of muscardine insecticide |
-
2009
- 2009-12-18 CN CN 200910264402 patent/CN101785482B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1395839A (en) * | 2002-05-13 | 2003-02-12 | 中国农业科学院生物防治研究所 | Preparing process and usage of muscardine insecticide |
Non-Patent Citations (3)
| Title |
|---|
| 何学友等.星天牛行为及控制技术研究Ⅱ.星天牛控制技术的研究.《福建林业科技》.2003,(第02期), * |
| 陈文进.石榴桃蛀螟的发生与综合防治研究.《河南科技大学学报(农学版)》.2004,(第02期), * |
| 黄金水等.白僵菌粘膏的研制和林间应用.《中国生物防治》.1990,(第03期), * |
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Address after: 223525 Jiangsu city of Lianyungang province guannaxian heap ditch town (Chemical Industrial Park) Patentee after: Jiangsu Gengyun Chemical Co., Ltd. Address before: 212126 Zhenjiang City, Jiangsu province Dantu District Bingzhen Rong Patentee before: Jiangsu Gengyun Chemical Co., Ltd. |