CN1134408A - Composite microbial fertilizer - Google Patents
Composite microbial fertilizer Download PDFInfo
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- CN1134408A CN1134408A CN 95104927 CN95104927A CN1134408A CN 1134408 A CN1134408 A CN 1134408A CN 95104927 CN95104927 CN 95104927 CN 95104927 A CN95104927 A CN 95104927A CN 1134408 A CN1134408 A CN 1134408A
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- Soil Conditioners And Soil-Stabilizing Materials (AREA)
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Abstract
Nitrogen-fixing bacteria, nitrogen-fixing fungi, phosphorus bacteria and potassium bacteria are cultured separately and fermented, and the fermented products are combined into composite microbe bacteria colony, which is then mixed with fly- ash, dried-fowl dung, fertile soil and other supplement into the said composite microbial fertilizer. The said product with rich bacteria and mould can make up the deficiencies of chemical fertilizer and single microbial fertilizer, and has strong nitrogen-fixing capacity and soil-improving function, so that it can result in obvious yield increase.
Description
One, a kind of new microbial fertilizer-composite microbiological fertilizer belongs to using microbe technology and field of fertilizer manufacturing.
Two, prior art and deficiency
Existing microbial fertilizer is monistic microbial fertilizer mostly.As: azotobacteria fertilizer, mainly form by bacterium with nitrogen fixation and fungi; And for example: phosphorus bacteria, its function are the organophosphoruss that decomposes in the soil, make it to become the phosphorus element that is easy to by plant absorbing; For another example: potassium bacterium, its function are the potassium compounds that decomposes in the soil, make it to become the available potassium that can be utilized by plant absorbing.These fertilizer all can be brought into play each self-applying when using separately, to satisfy plant to nitrogen, phosphorus, the needs of potassium three elements.They can not produce the soil compaction phenomenon that the life-time service chemical fertilizer is caused.But its deficiency is: use above-mentioned three quasi-microorganism fertilizer can only solve an aspect of plant desired nutritional separately, and cost is higher; Simultaneously, the three is used, again because of being difficult to the not enough or waste that the ratio of accomplishing suitably causes rate of fertilizer application.
Three, goal of the invention:
1, at the basic condition of China's soil fertility, particularly the basic content status of nitrogen, phosphorus, potassium three elements is wherein designed the plyability microbial fertilizer that contains vinelandii, phosphorus bacteria and potassium bacterium, to satisfy the comprehensive needs of plant to nitrogen, phosphorus, potassium.
2, solve present domestic nitrogenous fertilizer (chemical fertilizer) inadequate resource and expensive problem, reduce fertilizer cost, be beneficial to the development of agriculture production.
Four, core content of the present invention
With an elite strain nitrogen-fixing bacteria (aztobacter sp), one strain fixed nitrogen fungi (healthy and free from worry wood is mould), one strain has the phosphorus bacteria (phosphorus decomposing bacillus megaterium) and a strain potassium bacterium (frozen glue sample genus bacillus) of decomposing the organophosphorus function independently cultivates respectively, ferments, yet the ratio of determining in orthogonal experiment combines complex microbial community; As main component, again with flyash, auxiliary materials such as dry chicken manure and fertile soil mix composite microbiological fertilizer by a certain percentage with it.
Five, concrete implementation step of the present invention
(1) bacterial classification is made
As the complex microbial community of composite microbiological fertilizer main component, comprise four kinds of bacterial classifications of following three classes:
1. aztobacter sp (Azotobacter chroococcum),
2. healthy and free from worry wood mould (vinelandii) (Trichoderma k ningii),
3. phosphorus decomposing bacillus megaterium (phosphorus bacteria) (Bacillus megatherium
phosphaticum)，
4. frozen glue sample genus bacillus (potassium bacterium) (Bacillus mucilaginosus).
Now that these four kinds of bacterial classification making method divisions are as follows:
1, the cultivation of aztobacter sp
1. primary inclined plane is cultivated
Substratum major ingredient prescription is: sucrose 1%, dipotassium hydrogen phosphate 0.05%, lime carbonate 0.1%, sal epsom 0.02%, sodium-chlor 0.02%, agar 2%.PH7～7.2。
With the substratum test tube of packing into according to a conventional method; Sterilization; Inoculation; Cultivation preservation in 24 hours is standby down at 28 ± 1 ℃.
2. secondary triangular flask liquid culture
In one-level substratum major ingredient prescription, remove agar, as the secondary medium major ingredient, PH7～7.2.
Substratum is prepared the triangular flask of packing into, after sterilization, cooling, slant strains is inoculated in liquid nutrient medium; 28 ± 1 ℃ of following shaking table shaking culture 24 hours.When treating that cell count reaches 500,000,000/gram, stop cultivating, standby.
3. three grades of solid culture
Culture medium prescription is: wheat bran 40%, cottonseed cake 10%, rapeseed cake 10%, special soil 10%, Semen Maydis powder 10%, jowar 10%, fine rice bran 10%.
With the solid materials back profit water of weighing in proportion, sterilization, cooling, inoculation liquid second class inoculum; Under 28 ± 1 ℃ of conditions, cultivated 36～48 hours.Quality examination then, low temperature (below 60 ℃) drying is pulverized, and preserves standby.
2, the cultivation that the healthy and free from worry wood of vinelandii is mould
1. one-level is cultivated
Prescription adds nutrient agar for conventional potato.
The substratum of making is loaded on test tube, and inoculation after the routine sterilization was cultivated 3～4 days under 26 ± 1 ℃ of conditions; After waiting to grow green spores, stop cultivating, preserve standby.
2. secondary is cultivated
Culture medium prescription is: wheat bran 50%, Semen Maydis powder 40%, straw powder 10%.
After dry material moistened water, sterilization, cooling, the inoculation first class inoculum was cultivated in glass culture dish.Cultivating about 5 days under 26 ± 1 ℃ of conditions; After waiting to grow green spores, stop cultivating.Low temperature (below 60 ℃) drying is preserved standby.
3. three grades of cultivations
Culture medium prescription is with the secondary medium prescription.
After dry material moistened water, sterilization, cooling, the inoculation second class inoculum, utilization thick-layer ventilation technology was cultivated 3 days down at 28 ± 1 ℃; After waiting to grow green spores, stop cultivating.Standby after the cryodrying.
3, the cultivation of phosphorus decomposing bacillus megaterium
1. primary inclined plane is cultivated:
Culture medium prescription adds nutrient agar for conventional potato.Sterilization according to a conventional method, inoculation was cultivated 48 hours under 28 ± 1 ℃ of conditions, preserved standby.
2. secondary liquid culture:
In the one-level culture medium prescription, remove agar, as secondary medium.
With the substratum triangular flask of packing into, inoculate slant strains through autoclaving, cooling back; 28 ± 1 ℃ of following shaking table shaking culture 36 hours; When treating that cell count reaches 500,000,000/gram, stop cultivating, standby.
3. three grades of cultivations
Culture medium prescription is: wheat bran 30%, jowar 20%, Semen Maydis powder 40%, flyash 10%.
With dry material weigh back profit water, sterilization, cooling, connect second class inoculum, under 28 ± 1 ℃ of conditions, reach 48 hours through the tray solid culture, when treating that cell count reaches 4,000,000,000/gram, stop cultivating.Cryodrying and pulverizing are standby then.
4, the cultivation of potassium bacterium-frozen glue sample genus bacillus
1. primary inclined plane is cultivated
Substratum major ingredient prescription is: sucrose 1%, dipotassium hydrogen phosphate 0.05%, sal epsom 0.02%, magnesium chloride 0.02%, lime carbonate 0.1%, yeast extract paste 0.04%, agar 2%, PH7～7.2.
With the substratum test tube of packing into, sterilization according to a conventional method, inoculation was cultivated 24 hours under 28 ± 1 ℃ of conditions, preserved standby.
2. secondary liquid culture
In the one-level culture medium prescription, remove agar, as secondary medium.PH7～7.2。
With the prepared culture medium triangular flask of packing into, sterilization according to a conventional method, after the cooling, the inoculation slant strains.Under 28 ± 1 ℃ of conditions, leave standstill and cultivated 48 hours; When cell count reaches 1,000,000,000/when milliliter is above, stops cultivation.Preserve standby.
3. three grades of solid culture
Culture medium prescription is: wheat bran 30%, flyash 20%, plant ash 10%, Semen Maydis powder 30%, rapeseed cake 10%.
After dry material weighed, moistens water, sterilization, cooling in proportion, the inoculation second class inoculum.Adopt the solid anaerobic fermentation method 28 ± 1 ℃ of condition bottom fermentations 48 hours.When cell count reaches 12,000,000,000/gram, stop cultivating.Cryodrying is pulverized, and preserves standby.
(2) preparation of this fertilizer
The ratio that four kinds of microorganisms of cultured three classes are determined in orthogonal experiment is mixed, and promptly mould, the phosphorus decomposing bacillus megaterium of aztobacter sp, healthy and free from worry wood and four kinds of fermented product ratios of frozen glue sample genus bacillus are 15: 20: 30: 35.So the mixed strains of forming claims complex microbial community.
This composite flora accounts for 40% in composite microbiological fertilizer, other compositions are: flyash 30%, dry chicken manure 6%, fertile soil 24%.Composite flora is mixed with flyash, dried poultrymanure, fertile soil, and after stirring, pack, i.e. cost product.
Six, this product the key technical indexes and advantage
1, the key technical indexes
1. outward appearance and Oranoleptic indicator:
Beige.The fermented product special odor is arranged.
2. moisture content≤10%.
3. microbiological indicator
Total plate count 〉=1,600,000,000/gram; Total number of molds 〉=3.4 hundred million/gram.
2, advantage
1. this product is combined in the definite ratio of orthogonal experiment by two kinds of vinelandii and phosphorus bacteria, potassium bacterium, so can replenish the deficiency of existing chemical fertilizer and microbial fertilizer unicity, it is suitable for the plant growth in China's Different Soil.
2. this product substitutes part nitrogen, phosphorus, potassium fertilizer use, can reduce planting cost, improves output.Experiment shows, uses this product can improve grain yield 5～10%, improves vegetable crop about 5～20%, also can improve the quality and the local flavor of vegetables, grain.
3. the healthy and free from worry wood of a strain that is adopted is mould to be the azotobacter strain of introducing from U.S. Cornell University, and its culture process is simple, and this bacterium nitrogen fixing capacity is strong, and tangible improvement soil property and Soil structure are arranged, function such as keep soil from packing together.
Reference
1, Ceng Dingzhu: " diazotroph ", press of Xiamen University, 1987 the 11st edition.
2，Mascharak?PK?et?al(1983).J.Amer.chem.Soc.105.475-483
Claims (4)
1, a kind of new microbial fertilizer one composite microbiological fertilizer, it is characterized in that: an elite strain nitrogen-fixing bacteria, a strain fixed nitrogen fungi, a strain are had the phosphorus bacteria of decomposition organophosphorus function and the independent cultivation of potassium bacterium difference, the fermentation that a strain can be decomposed potassium compound, then tunning is combined as complex microbial community by specified proportion, by a certain percentage complex microbial community is mixed product as auxiliary materials such as main component and flyash, dry chicken manure and fertile soil at last.
2, according to the described composite microbiological fertilizer of claim 1, it is characterized in that: four kinds of bacterial strains of three classes such as said elite nitrogen-fixing bacteria, fixed nitrogen fungi, phosphorus bacteria and potassium bacterium respectively are:
1. aztobacter sp (Azotobacter chroococcum),
2. healthy and free from worry wood mould (vinelandii) (Trichoderma k ningii),
3. phosphorus decomposing bacillus megaterium (phosphorus bacteria) (Bacillus megatherium
phosphaticum)，
4. frozen glue sample genus bacillus (potassium bacterium) (Bacillus mucilaginosus).
3, according to claim 1 or 2 described composite microbiological fertilizers, it is characterized in that: the particular combinations ratio of said complex microbial community is meant the ratio of determining through orthogonal experiment, promptly four kinds of bacterium (with 1., 2., 3., 4. representative) ratio of tunning is:
①∶②∶③∶④＝15∶20∶30∶35。
4, according to claim 1 or 2 described composite microbiological fertilizers, it is characterized in that: said complex microbial community and flyash, dry chicken manure and fertile soil blended certain proportion are followed successively by 40%, 30%, 6% and 24%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95104927 CN1134408A (en) | 1995-04-28 | 1995-04-28 | Composite microbial fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95104927 CN1134408A (en) | 1995-04-28 | 1995-04-28 | Composite microbial fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1134408A true CN1134408A (en) | 1996-10-30 |
Family
ID=5075319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95104927 Pending CN1134408A (en) | 1995-04-28 | 1995-04-28 | Composite microbial fertilizer |
Country Status (1)
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|---|---|
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003014043A1 (en) * | 2001-07-31 | 2003-02-20 | Guoliang Zhao | High efficiency active bioorganic fertilizer and its production method |
| CN101239847B (en) * | 2008-03-07 | 2011-04-20 | 山东省农业科学院土壤肥料研究所 | Liquid composite microbial fertilizer and its preparation method |
| CN102531716A (en) * | 2012-01-09 | 2012-07-04 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Azotobacter-containing microbial fertilizer |
| CN103570384A (en) * | 2013-10-28 | 2014-02-12 | 济源市国龙生物科技有限公司 | Compound microbial fertilizer and production method thereof |
| CN105331544A (en) * | 2015-11-03 | 2016-02-17 | 红河学院 | Multifunctional trichoderma strain LY3 and preparation method for bacterium agent of multifunctional trichoderma strain |
| CN105567604A (en) * | 2016-02-16 | 2016-05-11 | 江苏中通生物科技有限公司 | Compound microorganism bacterium agent and preparation method thereof |
-
1995
- 1995-04-28 CN CN 95104927 patent/CN1134408A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003014043A1 (en) * | 2001-07-31 | 2003-02-20 | Guoliang Zhao | High efficiency active bioorganic fertilizer and its production method |
| CN101239847B (en) * | 2008-03-07 | 2011-04-20 | 山东省农业科学院土壤肥料研究所 | Liquid composite microbial fertilizer and its preparation method |
| CN102531716A (en) * | 2012-01-09 | 2012-07-04 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Azotobacter-containing microbial fertilizer |
| CN103570384A (en) * | 2013-10-28 | 2014-02-12 | 济源市国龙生物科技有限公司 | Compound microbial fertilizer and production method thereof |
| CN103570384B (en) * | 2013-10-28 | 2016-04-13 | 济源市国龙生物科技有限公司 | A kind of composite microbiological fertilizer and production method thereof |
| CN105331544A (en) * | 2015-11-03 | 2016-02-17 | 红河学院 | Multifunctional trichoderma strain LY3 and preparation method for bacterium agent of multifunctional trichoderma strain |
| CN105567604A (en) * | 2016-02-16 | 2016-05-11 | 江苏中通生物科技有限公司 | Compound microorganism bacterium agent and preparation method thereof |
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