CN102943059A - Preparing method of decomposition promoting and composting compound fermentation inoculants - Google Patents
Preparing method of decomposition promoting and composting compound fermentation inoculants Download PDFInfo
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- CN102943059A CN102943059A CN201210475310XA CN201210475310A CN102943059A CN 102943059 A CN102943059 A CN 102943059A CN 201210475310X A CN201210475310X A CN 201210475310XA CN 201210475310 A CN201210475310 A CN 201210475310A CN 102943059 A CN102943059 A CN 102943059A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to a preparing method of microbial inoculants and discloses a preparing method of decomposition promoting and composting compound fermentation inoculants. The preparing method of decomposition promoting and composting compound fermentation inoculants includes: collecting indigenous bacteria, culturing the indigenous bacteria in enlarge cultivation mode, culturing the solid inclined surface of bacillus stearothermophilus, culturing the bacillus stearothermophilus in enlarge cultivation mode, culturing the bacillus stearothermophilus in a fermentation tank in aerobic culture mode, mixing the obtained solid indigenous bacteria inoculants and the obtained bacillus stearothermophilus solid inoculants in weight percent of (2-3):1 to prepare the decomposition promoting and composting compound fermentation inoculants. The obtained decomposition promoting and composting compound fermentation inoculants are fertilized in composting substrate at the early stage, can fast improve pile temperature and reduce pile PH value, shortens pile fermentation earlier-stage time, promotes substrate microorganism movement, leads composts to enter the high temperature stage in the shortest time, achieves fast thorough decomposition through thermophilic bacteria movement and oxygen filling in stacked piles, and accordingly improves composting quality.
Description
Technical field
The present invention relates to a kind of preparation method of microbiobacterial agent, relate in particular to a kind of preparation method of short rotten compost complex ferment microbial inoculum.
Background technology
Along with the development of industrial or agricultural, the generation of municipal sludge, rubbish, feces of livestock and poultry grows with each passing day, harm ecotope and human health that some solid waste are serious.But from the angle analysis of the utilization of resources, these solid waste are take organic matter as main, and contain the nutritive ingredients such as a certain amount of nitrogen, phosphorus, potassium, calcium, magnesium, sulphur and various trace elements, can become good agricultural fertilizer through processing.Because compost is a kind of biology technological process, different steps at composting process is different to the microbial population that organic degraded plays a crucial role, the various parameters that will control in technological process are exactly that those are to the influential factor of microorganism, because they determine the degree of microbial activities, and then affect speed and the quality of compost.Yet traditional spontaneous fermentation is owing to kind and the limited amount of contained indigenous microorganism, and the restriction of technological condition for fermentation, and it is long to exist fermentation period, and the fertilizer efficiency of leavened prod is low, and environment is caused secondary pollution problems, has limited its large-scale application.
The microorganism of at present compost inoculation mostly belongs to mesothermal microbiobacterial agent, although they can shorten compost and start fermentation time, but because the microorganism that inoculates will be dead rapidly within the pliotherm period, therefore be cooldown period after the of short duration pliotherm period, the compost maturity time is still longer, and it is not thorough to become thoroughly decomposed, be difficult to reach the compost maturity effect of people's expection, how to promote to be rapidly heated at a kind of better inoculating microbe of the early stage access of compost, and reduce rapidly the pH value of compost, shorten time and the process of prior fermentation, to promote the growth of indigenous microorganism in the substrate, thereby improving the efficient of fiber degradation, is the new problem in present compost fermentation field.
Summary of the invention
The present invention is directed to the compost fermentation microbial inoculum that exists in the prior art easily cause the microorganism that inoculates will be within the pliotherm period rapidly dead, the compost maturity time is long, become thoroughly decomposed thoroughly, be difficult to reach the shortcoming of the compost maturity effect of people's expection, and a kind of facility, the effective preparation method of short rotten compost complex ferment microbial inoculum are provided.
In order to solve the problems of the technologies described above, the present invention is solved by following technical proposals:
A kind of preparation method of short rotten compost complex ferment microbial inoculum, the method use respectively indigenous bacterium, bacstearothermophilus (Bacillus stearothermophilus), the following culturing step of process:
First step: collection, enlarged culturing by indigenous bacterium make solid original inhabitants bacteria agent;
Second step: aerated culture is made the bacstearothermophilus solid fungicide in the solid slant culture by bacstearothermophilus, enlarged culturing, the fermentor tank;
Third step: the solid original inhabitants' bacteria agent that makes above-mentioned and bacstearothermophilus solid fungicide are by weight 2-3: 1 ratio is mixed and made into short rotten compost complex ferment microbial inoculum.
The collection of described indigenous bacterium be get the half-cooked boiled rices of some kilograms pack into clean wooden case, cover rice paper, airtight wooden case port, wooden case and partly imbed the vegetable plot, allow the indigenous bacterium of periphery slip in the rice, finally form indigenous bacterium bacterium colony at rice; The enlarged culturing of described indigenous bacterium be with the rice of indigenous bacterium bacterium colony and brown sugar according to weight ratio 2-3: 1 ratio mixes the jar sealing of packing into and places until become dense thick liquid state, mix the wheat bran that mixes through high-temperature sterilization according to 1: 1 ratio of weight ratio again, air-dry processing after the fermentation;
The prescription of the slant culture base fluid that described bacstearothermophilus solid slant culture is required is 8-12g peptone, 2-5g beef extract, 4-6g sodium-chlor, 14-16g agar, 1L distilled water; The prescription of the seed culture fluid that described bacstearothermophilus enlarged culturing is required is 8-12g peptone, 2-5g beef extract, 4-6g sodium-chlor, 1L distilled water; Described bacstearothermophilus aerated culture in fermentor tank is about to bacstearothermophilus after the enlarged culturing and is seeded in the fermentor tank that substratum is housed with the inoculum size of 4%-6% (V/V), cultured bacstearothermophilus liquid is inoculated into it in sterilization wheat bran according to 1: 1 ratio of weight ratio, then puts back to air-dry processing behind the continuation aerated culture fermentation 36-48h in the fermentor tank.
The short rotten compost complex ferment microbial inoculum of finally making according to above-mentioned steps accesses in early days at the compost substrate, can pile the body pH value with reducing by the fast lifting heap temperature, shorten the time in early stage of heap body fermentation, strengthen the activity of substrate microorganism, the chien shih compost enters hot stage when the shortest, logical oxygen becomes thoroughly decomposed to realize speed in activity by thermophile bacteria and the stacking, thereby promotes compost quality.
As preferably, the prescription of described slant culture base fluid is 10g peptone, 3g beef extract, 5g sodium-chlor, 15g agar, 1L distilled water; The prescription of the seed culture fluid that described bacstearothermophilus enlarged culturing is required is 10g peptone, 3g beef extract, 5g sodium-chlor, 1L distilled water;
As preferably, it is 18 ℃-22 ℃ that the rice of the indigenous bacterium bacterium colony of described band and brown sugar mix the temperature that the jar sealing of packing into places, and be 7-10 days storage period.
As preferably, the culture temperature of the solid slant culture of described bacstearothermophilus is 40-50 ℃, incubation time 16-24 hour.
As preferably, describedly with bacstearothermophilus from the operation steps that the solid slant culture base of preservation is seeded to enlarged culturing the seed culture fluid be: at the triangular flask of the 1000ml seed culture fluid 200ml that packs into, under 55 ℃, set rotating speed 160-240rpm with rotary shaker, cultivated 24-36 hour, liquid spawn after the cultivation is adopted the colony counting method counting, and bacterium is counted content and reaches 1 * 10
9Individual/more than the ml.
As preferably, described rotary shaker is set rotating speed 200rpm, cultivates 30 hours.
As preferably, the culture temperature of aerated culture is 55-65 ℃ in the described fermentor tank, and mixing speed is 30-60rpm, and air flow is 0.6-1.2m
3/ h, incubation time are 22-28 hour.
As preferably, described culture temperature is 60 ℃, and mixing speed is 45rpm, and air flow is 0.9m
3/ h, incubation time are 24 hours.
According to technical scheme of the present invention, adopt indigenous bacteria agent and bacstearothermophilus microbial inoculum in conjunction with being prepared into composite fungus agent, in early days access in compost, energy fast lifting heap temperature and reduction heap body pH value, shorten the time in early stage of heap body fermentation, strengthen the substrate microbial growth, thereby improve the efficient of fiber degradation, the chien shih compost enters hot stage when the shortest, activity by thermophile bacteria, realizing that speed becomes thoroughly decomposed, thereby promote compost quality, improve compost production efficiency.
Description of drawings
Fig. 1 is process flow diagram of the present invention.
Embodiment
Below in conjunction with accompanying drawing and embodiment the present invention is described in further detail:
Embodiment 1
As shown in Figure 1, a kind of preparation method of short rotten compost complex ferment microbial inoculum, the method is used respectively indigenous bacterium, bacstearothermophilus (Bacillus stearothermophilus), wherein this bacterial classification of bacstearothermophilus is available from middle common micro-organisms preservation administrative center, deposit number is CGMCC1.1866, the following culturing step of process:
First step: collection, enlarged culturing by indigenous bacterium make solid original inhabitants bacteria agent;
Second step: aerated culture is made the bacstearothermophilus solid fungicide in the solid slant culture by bacstearothermophilus, enlarged culturing, the fermentor tank;
Third step: the solid original inhabitants' bacteria agent that makes above-mentioned and bacstearothermophilus solid fungicide are mixed and made into short rotten compost complex ferment microbial inoculum by weight 2: 1 ratio.
The collection of described indigenous bacterium, the half-cooked boiled rice of namely the getting 1.5kg clean boxes of packing into, half-cooked boiled rice occupies 1/3rd of boxes internal volume, the length of clean boxes is that 30cm is wide for 20cm, high for 10cm, covers rice paper at half-cooked boiled rice, behind the airtight boxes port, 1/3 part of boxes volume is imbedded in the vegetable plot of the complete vegetables of firm results, allow the indigenous bacterium of periphery slip in the rice, through 5 days, form white indigenous bacterium bacterium colony and pink colour original inhabitants bacterium bacterium colony at rice;
The enlarged culturing of described indigenous bacterium, namely take out the rice of above-mentioned band original inhabitants bacterium bacterium colony, mix brown sugar according to 2: 1 ratio of weight ratio, pack into after mixing jar, cover rice paper, then sealing is placed on the place of 18 ℃ of temperature, approximately places about 7 days, the rice for the treatment of indigenous bacterium bacterium colony becomes dense thick liquid state with the mixture of brown sugar, mix the wheat bran that mixes through high-temperature sterilization according to 1: 1 ratio of weight ratio again, fermentation air-dry processing after 48 hours in jar makes solid original inhabitants bacteria agent;
The prescription of the slant culture base fluid that described bacstearothermophilus solid slant culture is required is 10g peptone, 3g beef extract, 5g sodium-chlor, 15g agar, 1L distilled water, get the bacstearothermophilus bacterial classification and access above-mentioned solid slant culture base, culture temperature is 40 ℃, incubation time 16 hours;
The prescription of the seed culture fluid that described bacstearothermophilus enlarged culturing is required is 10g peptone, 3g beef extract, 5g sodium-chlor, 1L distilled water, bacstearothermophilus is seeded to enlarged culturing the above-mentioned seed culture fluid from the solid slant culture base of preservation, concrete operation step is at the triangular flask of the every 1000ml inoculum 200ml that packs into, under the condition of 55 ℃ temperature, set rotating speed 160rpm, cultivated 24 hours with rotary shaker, liquid spawn after the cultivation is adopted the colony counting method counting, and bacterium is counted content and reaches 1 * 10
9Individual/more than the ml;
Aerated culture in the described fermentor tank, namely after the bacstearothermophilus enlarged culturing, liquid spawn is seeded to capacity that substratum is housed aerated culture in the fermentor tank of 5L take the inoculum size of 4% (V/V), 55 ℃ of culture temperature, mixing speed 30rpm, air flow 0.6m
3/ h, incubation time 22 hours is inoculated into cultured bacstearothermophilus liquid in the sterilization wheat bran with it according to 1: 1 ratio of weight ratio, then puts back to and continues aerated culture fermentation 48h in the fermentor tank, carry out at last air-dry processing, make the bacstearothermophilus solid fungicide.
Embodiment 2
As shown in Figure 1, a kind of preparation method of short rotten compost complex ferment microbial inoculum, the method use respectively indigenous bacterium, bacstearothermophilus (Bacillus stearothermophilus), the following culturing step of process:
First step: collection, enlarged culturing by indigenous bacterium make solid original inhabitants bacteria agent;
Second step: aerated culture is made the bacstearothermophilus solid fungicide in the solid slant culture by bacstearothermophilus, enlarged culturing, the fermentor tank;
Third step: the solid original inhabitants' bacteria agent that makes above-mentioned and bacstearothermophilus solid fungicide are mixed and made into short rotten compost complex ferment microbial inoculum by weight 3: 1 ratio.
The collection of described indigenous bacterium, the half-cooked boiled rice of namely the getting 2.5kg clean boxes of packing into, half-cooked boiled rice occupies 1/3rd of boxes internal volume, the length of clean boxes is that 30cm is wide for 20cm, high for 10cm, covers rice paper at half-cooked boiled rice, behind the airtight boxes port, 1/2 part of boxes volume is imbedded in the vegetable plot of the complete vegetables of firm results, allow the indigenous bacterium of periphery slip in the rice, through 8 days, form white indigenous bacterium bacterium colony and pink colour original inhabitants bacterium bacterium colony at rice;
The enlarged culturing of described indigenous bacterium, namely take out the rice of above-mentioned band original inhabitants bacterium bacterium colony, mix brown sugar according to 3: 1 ratio of weight ratio, pack into after mixing jar, cover rice paper, then sealing is placed on the place of 22 ℃ of temperature, approximately places about 10 days, rice with indigenous bacterium bacterium colony becomes dense thick liquid state with the mixture of brown sugar, mix the wheat bran that mixes through high-temperature sterilization according to 1: 1 ratio of weight ratio again, fermentation air-dry processing after 48 hours in jar makes solid original inhabitants bacteria agent;
The prescription of the slant culture base fluid that described bacstearothermophilus solid slant culture is required is 12g peptone, 5g beef extract, 6g sodium-chlor, 16g agar, 1L distilled water, get the bacstearothermophilus bacterial classification and access above-mentioned solid slant culture base, culture temperature is 50 ℃, incubation time 24 hours;
The prescription of the seed culture fluid that described bacstearothermophilus enlarged culturing is required is 10g peptone, 3g beef extract, 5g sodium-chlor, 1L distilled water, bacstearothermophilus is seeded to enlarged culturing the above-mentioned seed culture fluid from the solid slant culture base of preservation, concrete operation step is at the triangular flask of the 1000ml inoculum 200ml that packs into, under the condition of 55 ℃ temperature, set rotating speed 240rpm, cultivated 36 hours with rotary shaker, liquid spawn after the cultivation is adopted the colony counting method counting, and bacterium is counted content and reaches 1 * 10
9Individual/more than the ml;
Aerated culture in the described fermentor tank, namely after the bacstearothermophilus enlarged culturing, liquid spawn is seeded to capacity that substratum is housed aerated culture in the fermentor tank of 5L take the inoculum size of 6% (V/V), 65 ℃ of culture temperature, mixing speed 60rpm, air flow 1.2m3/h, incubation time 28 hours, the ratio of cultured bacstearothermophilus liquid according to 1: 1 is inoculated into it in sterilization wheat bran, then put back to and continue aerated culture fermentation 48h in the fermentor tank, carry out at last air-dry processing, make the bacstearothermophilus solid fungicide.
Embodiment 3
As shown in Figure 1, a kind of preparation method of short rotten compost complex ferment microbial inoculum, the method use respectively indigenous bacterium, bacstearothermophilus (Bacillus stearothermophilus), the following culturing step of process:
First step: collection, enlarged culturing by indigenous bacterium make solid original inhabitants bacteria agent;
Second step: aerated culture is made the bacstearothermophilus solid fungicide in the solid slant culture by bacstearothermophilus, enlarged culturing, the fermentor tank;
Third step: the solid original inhabitants' bacteria agent that makes above-mentioned and bacstearothermophilus solid fungicide are mixed and made into short rotten compost complex ferment microbial inoculum by weight 2.5: 1 ratio.
The collection of described indigenous bacterium, the half-cooked boiled rice of namely the getting 2kg clean boxes of packing into, half-cooked boiled rice occupies 1/3rd of boxes internal volume, the length of clean boxes is that 35cm is wide for 25cm, high for 12cm, covers rice paper at half-cooked boiled rice, behind the airtight boxes port, 1/2 part of boxes volume is imbedded in the vegetable plot of the complete vegetables of firm results, allow the indigenous bacterium of periphery slip in the rice, through 8 days, form white indigenous bacterium bacterium colony and pink colour original inhabitants bacterium bacterium colony at rice;
The enlarged culturing of described indigenous bacterium, namely take out the rice of above-mentioned band original inhabitants bacterium bacterium colony, mix brown sugar according to 2.5: 1 ratio of weight ratio, the jar of packing into after mixing, cover rice paper, then sealing is placed on the place of 20 ℃ of temperature, approximately placed about 8 days, rice with indigenous bacterium bacterium colony becomes dense thick liquid state with the mixture of brown sugar, mix according to 1: 1 ratio of weight ratio and mix the sterilization wheat bran, fermentation air-dry processing after 48 hours in jar, make solid original inhabitants bacteria agent, stand-by;
The prescription of the slant culture base fluid that described bacstearothermophilus solid slant culture is required is 10g peptone, 3g beef extract, 5g sodium-chlor, 15g agar, 1L distilled water, get the bacstearothermophilus bacterial classification and access above-mentioned solid slant culture base, culture temperature is 45 ℃, incubation time 20 hours;
The prescription of the seed culture fluid that described bacstearothermophilus enlarged culturing is required is 10g peptone, 3g beef extract, 5g sodium-chlor, 1L distilled water, bacstearothermophilus is seeded to enlarged culturing the above-mentioned seed culture fluid from the solid slant culture base of preservation, concrete operation step is at the triangular flask of the 1000ml inoculum 200ml that packs into, under the condition of 55 ℃ temperature, set rotating speed 200rpm, cultivated 30 hours with rotary shaker, liquid spawn after the cultivation is adopted the colony counting method counting, and bacterium is counted content and reaches 1 * 10
9Individual/more than the ml;
Aerated culture in the described fermentor tank, namely after the bacstearothermophilus enlarged culturing, liquid spawn is seeded to capacity that substratum is housed aerated culture in the fermentor tank of 5L take the inoculum size of 5% (V/V), 60 ℃ of culture temperature, mixing speed 45rpm, air flow 0.9m
3/ h, incubation time 24 hours is inoculated into the ratio of cultured bacstearothermophilus liquid according to 1: 1 in the sterilization wheat bran with it, then puts back to and continues aerated culture fermentation 48h in the fermentor tank, carry out at last air-dry processing, make the bacstearothermophilus solid fungicide.
In a word, the above only is preferred embodiment of the present invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to the covering scope of patent of the present invention.
Claims (8)
1. the preparation method of a short rotten compost complex ferment microbial inoculum, it is characterized in that: the method is used respectively indigenous bacterium, bacstearothermophilus (Bacillus stearothermophilus), this bacterial classification is available from middle common micro-organisms preservation administrative center, deposit number is CGMCC1.1866, the following culturing step of process:
First step: collection, enlarged culturing by indigenous bacterium make solid original inhabitants bacteria agent;
Second step: aerated culture is made the bacstearothermophilus solid fungicide in the solid slant culture by bacstearothermophilus, enlarged culturing, the fermentor tank;
Third step: the solid original inhabitants' bacteria agent that makes above-mentioned and bacstearothermophilus solid fungicide are by weight 2-3: 1 ratio is mixed and made into short rotten compost complex ferment microbial inoculum.
The collection of described indigenous bacterium be get the half-cooked boiled rices of some kilograms pack into clean wooden case, cover rice paper, airtight wooden case port, wooden case and partly imbed the vegetable plot, allow the indigenous bacterium of periphery slip in the rice, finally form indigenous bacterium bacterium colony at rice; The enlarged culturing of described indigenous bacterium be with the rice of indigenous bacterium bacterium colony and brown sugar according to weight ratio 2-3: 1 ratio mixes the jar sealing of packing into and places until become dense thick liquid state, mix the wheat bran that mixes through high-temperature sterilization according to 1: 1 ratio of weight ratio again, air-dry processing after the fermentation;
The prescription of the slant culture base fluid that described bacstearothermophilus solid slant culture is required is 8-12g peptone, 2-5g beef extract, 4-6g sodium-chlor, 14-16g agar, 1L distilled water; The prescription of the seed culture fluid that described bacstearothermophilus enlarged culturing is required is 8-12g peptone, 2-5g beef extract, 4-6g sodium-chlor, 1L distilled water; Described bacstearothermophilus aerated culture in fermentor tank is about to bacstearothermophilus after the enlarged culturing and is seeded in the fermentor tank that substratum is housed with the inoculum size of 4%-6% (V/V), cultured bacstearothermophilus liquid is inoculated into it in sterilization wheat bran according to 1: 1 ratio of weight ratio, then puts back to air-dry processing behind the continuation aerated culture fermentation 36-48h in the fermentor tank.
2. preparation method according to claim 1, it is characterized in that: the prescription of described slant culture base fluid is 10g peptone, 3g beef extract, 5g sodium-chlor, 15g agar, 1L distilled water; The prescription of the seed culture fluid that described bacstearothermophilus enlarged culturing is required is 10g peptone, 3g beef extract, 5g sodium-chlor, 1L distilled water.
3. preparation method according to claim 1 is characterized in that: it is 18 ℃-22 ℃ that the rice of the indigenous bacterium bacterium colony of described band and brown sugar mix the temperature that the jar sealing of packing into places, and be 7-10 days storage period.
4. preparation method according to claim 1, it is characterized in that: the culture temperature of the solid slant culture of described bacstearothermophilus is 40-50 ℃, incubation time 16-24 hour.
5. preparation method according to claim 1, it is characterized in that: describedly with bacstearothermophilus from the operation steps that the solid slant culture base of preservation is seeded to enlarged culturing the seed culture fluid be: at the triangular flask of the 1000ml seed culture fluid 200ml that packs into, under 55 ℃, set rotating speed 160-240rpm with rotary shaker, cultivated 24-36 hour, liquid spawn after the cultivation is adopted the colony counting method counting, and bacterium is counted content and reaches 1 * 10
9Individual/more than the ml.
6. preparation method according to claim 5 is characterized in that: described rotary shaker is set rotating speed 200rpm, cultivates 30 hours.
7. preparation method according to claim 1, it is characterized in that: the culture temperature of aerated culture is 55-65 ℃ in the described fermentor tank, and mixing speed is 30-60rpm, and air flow is 0.6-1.2m
3/ h, incubation time are 22-28 hour.
8. preparation method according to claim 7, it is characterized in that: described culture temperature is 60 ℃, and mixing speed is 45rpm, and air flow is 0.9m
3/ h, incubation time are 24 hours.
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| CN105585383A (en) * | 2013-09-12 | 2016-05-18 | 蒋盘君 | Preparation method of safe pollution-free compound fertilizer |
| CN106376473A (en) * | 2016-08-31 | 2017-02-08 | 南京国龙生物科技有限公司 | Preparation method of economic environment-friendly biological fermenting bed suitable for breeding pigs |
| CN106518328A (en) * | 2016-10-24 | 2017-03-22 | 烟台市昊星晟信息技术有限公司 | Organic fertilizer production method |
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| CN105585383A (en) * | 2013-09-12 | 2016-05-18 | 蒋盘君 | Preparation method of safe pollution-free compound fertilizer |
| CN105801242A (en) * | 2013-09-12 | 2016-07-27 | 蒋盘君 | Soil-protecting pollution-free composite fertilizer and preparation method thereof |
| CN103614314A (en) * | 2013-10-12 | 2014-03-05 | 北京市捷博特能源技术有限公司 | Oil recovery microorganism composition |
| CN106376473A (en) * | 2016-08-31 | 2017-02-08 | 南京国龙生物科技有限公司 | Preparation method of economic environment-friendly biological fermenting bed suitable for breeding pigs |
| CN106518328A (en) * | 2016-10-24 | 2017-03-22 | 烟台市昊星晟信息技术有限公司 | Organic fertilizer production method |
| CN106518326A (en) * | 2016-10-24 | 2017-03-22 | 烟台市昊星晟信息技术有限公司 | A production method of a fertilizer having an antibiotic degrading effect |
| CN106518327A (en) * | 2016-10-24 | 2017-03-22 | 烟台市昊星晟信息技术有限公司 | Method for preparing compost with effect of removing antibiotics |
| CN107446866A (en) * | 2017-09-27 | 2017-12-08 | 福建省农科农业发展有限公司 | A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement |
| CN107653217A (en) * | 2017-11-25 | 2018-02-02 | 毕铭桓 | A kind of fermentation of bacillus subtilis mating system |
| CN110452032A (en) * | 2019-08-01 | 2019-11-15 | 中施柯朗（天津）污泥治理处理工程股份有限公司 | A kind of compost fermentation method |
| CN115029281A (en) * | 2022-07-08 | 2022-09-09 | 中国农业大学 | Preparation method of high-temperature-resistant cellulose degradation microbial inoculum and application of high-temperature-resistant cellulose degradation microbial inoculum in compost |
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Application publication date: 20130227 |