CN107653217A - A kind of fermentation of bacillus subtilis mating system - Google Patents
A kind of fermentation of bacillus subtilis mating system Download PDFInfo
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- CN107653217A CN107653217A CN201711198407.XA CN201711198407A CN107653217A CN 107653217 A CN107653217 A CN 107653217A CN 201711198407 A CN201711198407 A CN 201711198407A CN 107653217 A CN107653217 A CN 107653217A
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- fermentation
- bacillus subtilis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of method that fermentation of bacillus subtilis is bred, the present invention uses rice as culture medium, being there is provided with brown sugar between crude fibre and energy, the growth requirement of the suitable Bacillus subtillis of carbon-nitrogen ratio of rice, while rice cook the rear grain of rice has certain space, it can adapt to the aerobic requirement of Bacillus subtillis, simultaneously by spontaneous fermentation, need not be heated up in production process, insulating process, there is no extra energy expenditure, reduce production cost；And bacillus subtilis high concentration is kept in fermentation process, bacterium colony is with the obvious advantage, effectively inhibits the growth of miscellaneous bacteria, solves the problems, such as easily pollution in reproductive process.
Description
Technical field
The present invention relates to microorganism probiotic fermentation to breed field, and in particular to a kind of fermentation of bacillus subtilis side of breeding
Method.
Background technology
The problem of causing drug resistance with abuse of antibiotics medicine, is increasingly severe, and the advantage of probiotics gradually appears, prebiotic
Bacterium has the advantages that improving body immunity, preventing enteric infection.Bacillus subtilis is widely used at present, withered grass bud
Spore bacillus is one kind of bacillus, is widely distributed in soil and the organic matter of corruption, is easily bred in withered grass soaks juice, thus
Gain the name.Produce subtilin isoreactivity material during bacillus subtilis thalli growth, these active materials to germ processed or
The germ of autogenous infection has inhibitory action.Bacillus subtilis can keep activity in acidic stomach environment simultaneously, be resistant to
Saliva and the attack of bile, intestine and small intestine being all arrived at, bacillus subtilis speed consumes free oxygen in enteron aisle, causes enteron aisle hypoxemia,
Promote, beneficial to anaerobism bacteria growing, to suppress other pathogen growths indirectly.Immunoglobulin and antibody level are stimulated, strengthens cellular immunity
And humoral immune function, improve herd immunity.
But current market sales of bacillus subtilis formulation price production cost is too high, and existing mating system is present
The problem of easily polluting.
The content of the invention
The technical problem to be solved in the invention is to provide a kind of method that fermentation of bacillus subtilis is bred, and solves present
Commercially available bacillus subtilis formulation price production cost is too high, and mating system has the problem of easily pollution.
Technical scheme step is as follows：
Take rice cooked rice steamed to be in advance fitted into solid-state fermentation tank, treat that rice cooked rice is cooled to 40 DEG C or so, add withered grass bud
Spore bacillus bacterium solution（Bacillus subtilis powder agent is dissolved with a small amount of warm water）Stir, upper lid moistening gauze, keep humidity, room temperature
Spontaneous fermentation.
After fermentation 48 hours, a material was stirred every 12 hours.
Treat that culture is substantially glued wet to material, when having the distinctive smell of denseer Bacillus subtillis, continues to cultivate 48 hours.
Above-mentioned cultured rice is pressed 2:1 ratio stirs with brown sugar, lucifuge spontaneous fermentation.
When material fermentation is in a liquid state, week age or so is about needed, by material filtering, filtrate is high concentration withered grass gemma
Bacillus liquid.
This method has the beneficial effect that：The carbon-nitrogen ratio of rice is adapted to the growth requirement of Bacillus subtillis, while rice steams
There is certain space between the grain of rice after ripe, can adapt to the aerobic requirement of Bacillus subtillis, while pass through spontaneous fermentation, production
During need not heat up, insulating process, without extra energy expenditure, reduce production cost；Simultaneously one in fermentation process
Straight to keep bacillus subtilis high concentration, bacterium colony is with the obvious advantage, effectively inhibits the growth of miscellaneous bacteria, solves in reproductive process
The problem of easily polluting.
Embodiment
In order to be better understood from the present invention, with embodiment, the present invention will be further described below.
Bacillus subtilis kind daughter bacteria culture：Take rice cooked rice steamed to be in advance fitted into solid-state fermentation tank, treat that rice is done
Meal is cooled to 40 DEG C or so, adds bacillus subtilis bacterium solution（Bacillus subtilis powder agent is dissolved with a small amount of warm water）Stirring is equal
It is even, upper lid moistening gauze, keep humidity, room temperature spontaneous fermentation.
After fermentation 48 hours, a material was stirred every 12 hours.
Treat that culture is substantially glued wet to material, when having the distinctive smell of denseer Bacillus subtillis, continues to cultivate 48 hours.
Bacillus subtilis scale breeding：Above-mentioned cultured rice is pressed 2:1 ratio stirs with brown sugar, lucifuge
Spontaneous fermentation.
When material fermentation is in a liquid state, week age or so is about needed, by material filtering, filtrate is high concentration withered grass gemma
Bacillus liquid.
Claims (3)
1. a kind of method that fermentation of bacillus subtilis is bred, it is characterised in that using bacillus subtilis kind daughter bacteria culture and withered
Careless bacillus scale breeding.
2. the method that a kind of fermentation of bacillus subtilis according to claim 1 is bred, it is characterised in that described withered grass
Bacillus kind daughter bacteria culture is to take rice cooked rice steamed to be in advance fitted into solid-state fermentation tank, treats that rice cooked rice is cooled to 40 DEG C
Left and right, add bacillus subtilis bacterium solution（Bacillus subtilis powder agent is dissolved with a small amount of warm water）Stir, upper lid moistening yarn
Cloth, humidity is kept, room temperature spontaneous fermentation, after fermenting 48 hours, a material was stirred every 12 hours, treats that culture is obvious to material
It is viscous wet, when having the distinctive smell of denseer Bacillus subtillis, continue culture 48 hours.
3. the method that a kind of fermentation of bacillus subtilis according to claim 1 is bred, it is characterised in that described withered grass
Bacillus scale breeding is that above-mentioned cultured rice is pressed into 2:1 ratio stirs with brown sugar, lucifuge spontaneous fermentation, treats
When material fermentation is in a liquid state, week age or so is about needed, by material filtering, filtrate is high concentration bacillus subtilis bacterium solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711198407.XA CN107653217A (en) | 2017-11-25 | 2017-11-25 | A kind of fermentation of bacillus subtilis mating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711198407.XA CN107653217A (en) | 2017-11-25 | 2017-11-25 | A kind of fermentation of bacillus subtilis mating system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107653217A true CN107653217A (en) | 2018-02-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711198407.XA Pending CN107653217A (en) | 2017-11-25 | 2017-11-25 | A kind of fermentation of bacillus subtilis mating system |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002161045A (en) * | 2000-09-18 | 2002-06-04 | Toyo Hakko:Kk | Ameliorant for alcohol metabolism and reducing agent for hepatopathy |
| CN101993829A (en) * | 2009-08-25 | 2011-03-30 | 吉林师范大学 | Composite microbial agent |
| CN102352334A (en) * | 2011-10-21 | 2012-02-15 | 山东省农业科学院高新技术研究中心 | Method for producing viable bacillus subtilis by way of solid fermentation |
| CN102943059A (en) * | 2012-11-22 | 2013-02-27 | 兰州大学 | Preparing method of decomposition promoting and composting compound fermentation inoculants |
| CN103937700A (en) * | 2014-02-28 | 2014-07-23 | 绵竹市丰源谷物种植专业合作社 | Composite biological agent used for restoring cadmium polluted soil, and its preparation method |
-
2017
- 2017-11-25 CN CN201711198407.XA patent/CN107653217A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002161045A (en) * | 2000-09-18 | 2002-06-04 | Toyo Hakko:Kk | Ameliorant for alcohol metabolism and reducing agent for hepatopathy |
| CN101993829A (en) * | 2009-08-25 | 2011-03-30 | 吉林师范大学 | Composite microbial agent |
| CN102352334A (en) * | 2011-10-21 | 2012-02-15 | 山东省农业科学院高新技术研究中心 | Method for producing viable bacillus subtilis by way of solid fermentation |
| CN102943059A (en) * | 2012-11-22 | 2013-02-27 | 兰州大学 | Preparing method of decomposition promoting and composting compound fermentation inoculants |
| CN103937700A (en) * | 2014-02-28 | 2014-07-23 | 绵竹市丰源谷物种植专业合作社 | Composite biological agent used for restoring cadmium polluted soil, and its preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 张欢欢: "三株有益菌的初步分离鉴定及芽孢杆菌在生物絮团对虾养殖中的应用", 《万方学位论文》 * |
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Application publication date: 20180202 |