CN107129947A - A kind of zymotechnique for the specialty regulation enteron aisle compound bacteria being used in aquaculture - Google Patents
A kind of zymotechnique for the specialty regulation enteron aisle compound bacteria being used in aquaculture Download PDFInfo
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- CN107129947A CN107129947A CN201710329033.4A CN201710329033A CN107129947A CN 107129947 A CN107129947 A CN 107129947A CN 201710329033 A CN201710329033 A CN 201710329033A CN 107129947 A CN107129947 A CN 107129947A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K40/00—Shaping or working-up of animal feeding-stuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The present invention is prepared culture medium, then mixes two kinds of strains, finally add in culture medium mixed bacteria and carry out fermented and cultured first using the specialty regulation enteron aisle compound bacteria in above-mentioned technique productions aquaculture.The product bacterium number content that fermenting and producing comes out is 13 × 108Cfu/ml or more.Specialty regulation enteron aisle compound bacteria technique in this method production aquaculture is simple, and process rationally, is formed by lactic acid bacteria, saccharomycete scientific compatibility, producing enzyme is balanced, aquiculture animal gastrointestinol microorganism colony balance can be regulated and controled, strengthen immunity can also improve the activity of aquatic livestock digestive ferment, so as to improve its digestion power to feed, whet the appetite, and then improve Feeding Velocity, reduce the loss of nutriment, feed efficiency is improved, with more wide market prospects and promotional value.
Description
Technical field
The present invention relates to for biofermentation technique, specifically a kind of specialty regulation enteron aisle being used in aquaculture is answered
Close the zymotechnique of bacterium.
Background technology
Culture fishery is an important economic industry of China, and the fast development of culture fishery is the hair of China's economy
Exhibition is made that important contribution.The problem of culture fishery also encounters various while China flourishes, germplasm
Degeneration, the outburst of disease large area, feeding quality raising etc. all seriously annoying the further development of culture fishery.It is wherein annual
Because the large area of some diseases breaks out, the cultivation to aquatic livestock causes huge loss, as restriction culture fishery hair
One key factor of exhibition.
After disease generation, we generally select some antibiotic or chemicals to resist the invasion of bacterium or virus, though
The effect for eliminating pathogen can be so reached, but with the long-term use of a large amount of antibiotic and chemicals, is also supported to aquatic products
Grow the unsafe factor that industry brings many.Antibiotics is used for a long time, aquaculture object drug resistance is easily caused
Produce, the immunity function of itself declines, and easily cause the variation of pathogen, produce many endurance strains, be that aquatic products is supported
The preventing and treating for growing object disease hides some dangers for.Secondly antibiotic, the problem of medicament residue being produced, aquaculture dealer is used for a long time
In order to reach the purpose of prevention and control disease generation, not in accordance with the regulation about the off-drug period, some are continuing with during the off-drug period and is resisted
Raw element class medicine, causes medicament residue, and then produces food-safety problem, and influence consumer's is healthy.
At present, in order to solve the problem of abuse of antibiotics during aquaculture of aquatic animal is caused, especially food security is asked
Topic, seeks the research of a kind of green, safety, the method for non-harmful controlling disease as focus, wherein the side for passing through microorganism
Method improves the immunity function of aquiculture animal, reaches that the method for disease prevention and control is paid close attention to by Many researchers, as solution
The certainly effective way of disease and safety issue.
Probiotics is a class by improving Host Strains group composition so as to play the active microorganism of beneficial effect.Application at present
Probiotics in aquaculture has photosynthetic bacteria, bud pole bacterium (bacillus subtilis and lichens bud pole bacterium), saccharomycete (ferment
Female culture and active dry yeast), a few species such as lactic acid bacteria (lactobacterium acidophilus and Bifidobacterium).
The field planting antagonism of probiotics.Probiotics can be colonized in an orderly manner the surfaces such as animal mucous membrane, skin or cell it
Ask, occupy the field planting site in Host Digestion road, form biological barrier, reduce infecting, being colonized for pathogenic microorganism, play occupy-place,
Nutrition, the effect of antagonism are fought for, body is small is invaded by pathogen for protection.
Probiotics can suppress the effect of pathogen.Probiotics can suppress field planting and the propagation of pathogenic bacteria, except in quantity
Advantage outside, can also suppress the growth and breeding of pathogen by secreting antibacterial material.Such as Bifidobacterium, lactic acid bacteria in vivo
Energy fermenting carbohydrate produces acetic acid and lactic acid, enteral is in sour environment, forms enteron aisle chemoproection barrier, can protect enteron aisle
Interior microenvironment, hinders the intrusion than pathogenic bacteria, adhesion and is colonized, suppress abnormal microflora such as shigella dysenteriae, typhoid bacillus, deformation
Bacillus, the growth of Pseudomonas aeruginosa and fungi and its generation of Toxic Metabolites.Probiotics can also by producing extracellular glycosidase,
Prevent invasion and attack of the pathogenic bacteria to protection of intestinal mucosal barrier cells.
Probiotics also has trophism.Probiotics is using specific some enzyme (such as galactosidase itself)
Deficiency of the host on digestive ferment is supplemented, help decomposes the nutriment that upper digestive tract is not fully hydrolyzed absorption, is conducive to place
Master further absorbs.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of technique simply, and process is reasonable, by lactic acid bacteria, Saccharomycetaceae
Learn compatibility to form, producing enzyme is balanced, can regulate and control aquiculture animal gastrointestinol microorganism colony balance, strengthen immunity may be used also
To improve the activity of aquatic livestock digestive ferment, so as to improve its digestion power to feed, whet the appetite, and then improve speed of ingesting
Degree, reduces the loss of nutriment, improves feed efficiency.
The present invention solve above-mentioned technical problem use technical scheme be:A kind of specialty regulation intestines being used in aquaculture
The zymotechnique of road compound bacteria, it is characterised in that:Concrete technology step is:
A. the preparation of culture medium:Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water:Seawater=50:15:15:20:6:20:1000:
9437:9437, it is standby that nutrient solution is made in fully dissolving;
B. the preparation of composite probiotics preparations:Lactobacillus acidophilus in extracting lactic acid bacterium, strain content is 5 × 108Cfu/ml or with
On;Saccharomyces cerevisiae in saccharomycete, strain content is 2 × 108Cfu/ml or more;6-8 parts of extracting lactic acid bacterium, takes saccharomycete 2-4
Part, add in mixer and mix 20-30 minutes, be well mixed;
C. the preparation of compound bacteria:Take nutrient solution obtained by strain obtained by step b and step a by volume 1:8-9 ratio pours into hair
In fermentation tank, compound bacteria is made in 25-30 DEG C of keeping temperature, anaerobic fermentation culture 6-10 days.
The present invention prepares culture medium first using the specialty regulation enteron aisle compound bacteria in above-mentioned technique productions aquaculture,
Then two kinds of strains are mixed, finally mixed bacteria is added in culture medium and carries out fermented and cultured.The product bacterium that fermenting and producing comes out
Number content is 13 × 108Cfu/ml or more.Specialty regulation enteron aisle compound bacteria technique in this method production aquaculture is simple,
Process rationally, is formed by lactic acid bacteria, saccharomycete scientific compatibility, and producing enzyme is balanced, can regulate and control the micro- life of aquiculture animal intestines and stomach
Thing colony balance, strengthen immunity can also improve the activity of aquatic livestock digestive ferment, so as to improve its digestible energy to feed
Power, whets the appetite, and then improves Feeding Velocity, reduces the loss of nutriment, feed efficiency is improved, with more wide city
Field prospect and promotional value.
Embodiment
With reference to embodiment, the invention will be further described.
A kind of zymotechnique of specialty regulation enteron aisle compound bacteria in aquaculture of the present invention, it comprises the following steps:
A. the preparation of culture medium:Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water:Seawater=50:15:15:20:6:20:1000:
9437:9437, it is standby that nutrient solution is made in fully dissolving;
B. the preparation of composite probiotics preparations:Lactobacillus acidophilus in extracting lactic acid bacterium, strain content is 5 × 108Cfu/ml or with
On;Saccharomyces cerevisiae in saccharomycete, strain content is 2 × 108Cfu/ml or more;6-8 parts of extracting lactic acid bacterium, takes saccharomycete 2-4
Part, add in mixer and mix 20-30 minutes, be well mixed;
C. the preparation of compound bacteria:Take nutrient solution obtained by strain obtained by step b and step a by volume 1:8-9 ratio pours into hair
In fermentation tank, compound bacteria is made in 25-30 DEG C of keeping temperature, anaerobic fermentation culture 6-10 days.
Embodiment 1:
A kind of zymotechnique of specialty regulation enteron aisle compound bacteria in aquaculture, it comprises the following steps:
A. the preparation of culture medium:Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water:Seawater=50:15:15:20:6:20:1000:
9437:9437, it is standby that nutrient solution is made in fully dissolving;
B. the preparation of composite probiotics preparations:Lactobacillus acidophilus in extracting lactic acid bacterium, strain content is 5 × 108Cfu/ml or with
On;Saccharomyces cerevisiae in saccharomycete, strain content is 2 × 108Cfu/ml or more;6 parts of extracting lactic acid bacterium, takes 4 parts of saccharomycete, plus
Enter in mixer and mix 20 minutes, be well mixed;
C. the preparation of compound bacteria:Take nutrient solution obtained by strain obtained by step b and step a by volume 1:8 ratio pours into fermentation
In tank, compound bacteria is made in 25-30 DEG C of keeping temperature, anaerobic fermentation culture 6 days.Bacterium number content is 8 × 108Cfu/ml, with hair
Ferment acid fragrance.
Example 2:
A. the preparation of culture medium:Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water:Seawater=50:15:15:20:6:20:1000:
9437:9437, it is standby that nutrient solution is made in fully dissolving;
B. the preparation of composite probiotics preparations:Lactobacillus acidophilus in extracting lactic acid bacterium, strain content is 5 × 108Cfu/ml or with
On;Saccharomyces cerevisiae in saccharomycete, strain content is 2 × 108Cfu/ml or more;7 parts of extracting lactic acid bacterium, takes 3 parts of saccharomycete, plus
Enter in mixer and mix 25 minutes, be well mixed;
C. the preparation of compound bacteria:Take nutrient solution obtained by strain obtained by step b and step a by volume 1:8 ratio pours into fermentation
In tank, compound bacteria is made in 25-30 DEG C of keeping temperature, anaerobic fermentation culture 8 days.Bacterium number content is 11 × 108Cfu/ml, has
The sour fragrance of stronger fermentation.
Example 3:
A. the preparation of culture medium:Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water:Seawater=50:15:15:20:6:20:1000:
9437:9437, it is standby that nutrient solution is made in fully dissolving;
B. the preparation of composite probiotics preparations:Lactobacillus acidophilus in extracting lactic acid bacterium, strain content is 5 × 108Cfu/ml or with
On;Saccharomyces cerevisiae in saccharomycete, strain content is 2 × 108Cfu/ml or more;8 parts of extracting lactic acid bacterium, takes 2 parts of saccharomycete, plus
Enter in mixer and mix 30 minutes, be well mixed;
C. the preparation of compound bacteria:Take nutrient solution obtained by strain obtained by step b and step a by volume 1:9 ratio pours into fermentation
In tank, compound bacteria is made in 25-30 DEG C of keeping temperature, anaerobic fermentation culture 10 days.Bacterium number content is 13 × 108Cfu/ml, has
The sour fragrance of strong fermentation.
Claims (4)
1. a kind of zymotechnique of specialty regulation enteron aisle compound bacteria in aquaculture, it is characterised in that:It includes following step
Suddenly:
A. the preparation of culture medium:Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water:Seawater=50:15:15:20:6:20:1000:
9437:9437, it is standby that nutrient solution is made in fully dissolving;
B. the preparation of composite probiotics preparations:Lactobacillus acidophilus in extracting lactic acid bacterium, strain content is 5 × 108Cfu/ml or with
On;Saccharomyces cerevisiae in saccharomycete, strain content is 2 × 108Cfu/ml or more;6-8 parts of extracting lactic acid bacterium, takes saccharomycete 2-4
Part, add in mixer and mix 20-30 minutes, be well mixed;
C. the preparation of compound bacteria:Take nutrient solution obtained by strain obtained by step b and step a by volume 1:8-9 ratio pours into hair
In fermentation tank, compound bacteria is made in 25-30 DEG C of keeping temperature, anaerobic fermentation culture 6-10 days.
2. the zymotechnique of the specialty regulation enteron aisle compound bacteria in aquaculture according to claim 1, it is characterised in that:
Sal-ammoniac (NH is selected in the step a4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water:Seawater=50:15:15:20:6:20:1000:
9437:9437, it is standby that nutrient solution is made in fully dissolving.
3. the zymotechnique of the specialty regulation enteron aisle compound bacteria in aquaculture according to claim 1, it is characterised in that:
Lactobacillus acidophilus in the step b in extracting lactic acid bacterium, strain content is 5 × 108Cfu/ml or more;Wine brewing in saccharomycete
Yeast, strain content is 2 × 108Cfu/ml or more;6-8 parts of extracting lactic acid bacterium, takes 2-4 parts of saccharomycete, adds in mixer and mixes
Close 20-30 minutes, be well mixed.
4. the zymotechnique of the specialty regulation enteron aisle compound bacteria in aquaculture according to claim 1, it is characterised in that:
Strain and nutrient solution by volume 1 in the step c:8-9 ratio is poured into fermentation tank, 25-30 DEG C of keeping temperature, anaerobism hair
Ferment culture 6-10 days, is made compound bacteria.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111606426A (en) * | 2020-06-08 | 2020-09-01 | 济南素帜生物技术有限公司 | Preparation method of complex microbial inoculant for aquatic products |
CN112126591A (en) * | 2020-05-28 | 2020-12-25 | 安吉国千环境科技有限公司 | Culture medium formula for kitchen waste recycling composite flora culture and culture method thereof |
-
2017
- 2017-05-11 CN CN201710329033.4A patent/CN107129947A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112126591A (en) * | 2020-05-28 | 2020-12-25 | 安吉国千环境科技有限公司 | Culture medium formula for kitchen waste recycling composite flora culture and culture method thereof |
CN111606426A (en) * | 2020-06-08 | 2020-09-01 | 济南素帜生物技术有限公司 | Preparation method of complex microbial inoculant for aquatic products |
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Application publication date: 20170905 |