CN107494889A - The preparation method of bacillus subtilis liquid fermentation feed - Google Patents
The preparation method of bacillus subtilis liquid fermentation feed Download PDFInfo
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- CN107494889A CN107494889A CN201610421452.6A CN201610421452A CN107494889A CN 107494889 A CN107494889 A CN 107494889A CN 201610421452 A CN201610421452 A CN 201610421452A CN 107494889 A CN107494889 A CN 107494889A
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- Prior art keywords
- bacillus subtilis
- feed
- preparation
- fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
According to ratio of water to material it is (2.5~3) this application discloses a kind of preparation method of bacillus subtilis liquid fermentation feed, including by basal feed and water：1 liquid feed inoculation fermentation seed liquor, is enclosed in sealing container and carries out anaerobic fermentation.The present invention, compared to traditional siccative and wet-mixing material, can be substantially reduced feed cost, improve nutritive digestibility in feed, can reduce animal gastrointestinal tract pH value, suppressed pathogenic microorganism breeding, promote animal health using liquid fermentation feed.
Description
Technical field
The application is related to a kind of preparation method of bacillus subtilis liquid fermentation feed.
Background technology
With the increasingly raising of living standard, people increasingly pay close attention to the safety problem of meat, and in meat products
Abuse, residual and the drug resistance problems of antibiotic turn into the topic that people pay close attention to.Do not adding antibiotic, growth accelerator etc.
Under the conditions of additive, improving livestock products quality turns into the problem of in the urgent need to address at present.
Liquid fermentation feed refers to ferment to feed using microorganism, obtains a kind of liquid feed.As a kind of new
Type safe feed, liquid fermentation feed can degrade aflatoxin that may be present in feed, suppress Escherichia coli Growth, carry
High phosphorus utilization, reduce the effect such as grice diarrhoea rate.
The content of the invention
It is existing to overcome it is an object of the invention to provide a kind of preparation method of bacillus subtilis liquid fermentation feed
Deficiency in technology.
To achieve the above object, the present invention provides following technical scheme：
The embodiment of the present application discloses a kind of preparation method of bacillus subtilis liquid fermentation feed, including：
(1), that bacillus subtilis is inoculated into bacillus subtilis bacterium culture medium at 28~30 DEG C into culture 24~30 is small
When, fermentation seed liquid is made, bacillus subtilis bacterium culture medium includes according to parts by weight：2~3 parts of beef extract；Peptone 8~10
Part；4~5 parts of sodium chloride；0.1~0.2 part of potassium dihydrogen phosphate；0.03~0.06 part of magnesium sulfate；18~20 parts of agar；Water 1000
Part；
(2) it is, (2.5~3) according to ratio of water to material by basal feed and water：1 liquid feed inoculation fermentation seed liquor, load
Anaerobic fermentation is carried out in sealing container.
Preferably, in the preparation method of above-mentioned bacillus subtilis liquid fermentation feed, basal feed is according to weight
Than including：
Corn 60~65%；
Dregs of beans 19~22%；
Wheat bran 7~8%；
Fish meal 3~4%
Surplus is vitamin and trace element.
Preferably, in the preparation method of above-mentioned bacillus subtilis liquid fermentation feed, the fermentation seed liquid
Inoculum concentration is 3%.
Preferably, in the preparation method of above-mentioned bacillus subtilis liquid fermentation feed, the anaerobic fermentation time 60 is small
When, 30 DEG C of fermentation temperature.
Preferably, in the preparation method of above-mentioned bacillus subtilis liquid fermentation feed, the bacillus subtilis
The pH value of culture medium is 7.0~7.2.
Compared with prior art, the advantage of the invention is that：The present invention is using liquid fermentation feed compared to traditional siccative
With wet-mixing material, feed cost can be substantially reduced, nutritive digestibility in feed is improved, animal gastrointestinal tract pH can be reduced
Value, suppress pathogenic microorganism breeding, promote animal health.
Embodiment
The present invention is described further by the following example：According to following embodiments, the present invention may be better understood.
However, as it will be easily appreciated by one skilled in the art that specific material ratio, process conditions and its result described by embodiment are only used
In the explanation present invention, without should be also without limitation on the present invention described in detail in claims.
The preparation method of bacillus subtilis liquid fermentation feed：
Bacillus subtilis bacterium culture medium, includes according to parts by weight：
3 parts of beef extract；10 parts of peptone；5 parts of sodium chloride；0.1 part of potassium dihydrogen phosphate；0.05 part of magnesium sulfate；20 parts of agar；
1000 parts of water.
The pH value of bacillus subtilis bacterium culture medium is 7.0~7.2.
Bacillus subtilis is inoculated into bacillus subtilis bacterium culture medium and cultivated 24 hours at 30 DEG C, fermentation kind is made
Sub- liquid.
It is 2.5 by ratio of water to material：The fermentation seed liquid of 1 liquid feed inoculation 3%, is enclosed in sealing container and carries out anaerobism
Fermentation, fermentation time 60 hours, 30 DEG C of fermentation temperature.
Basal feed includes according to weight ratio：
Corn 65%；
Dregs of beans 19%；
Wheat bran 8%；
Fish meal 4%
Surplus is vitamin and trace element.
Relative to situation of not fermenting, crude protein content brings up to 19.37%/DM by 18%/DM before fermenting in liquid feed,
Crude fiber content drops to 2.17%/DM, and lactic acid content has reached 18g/L/DM, and biomass is 130 × 106Cfu/ml, it is antibacterial
Rate reaches more than 96%.
Finally, it is to be noted that, term " comprising ", "comprising" or its any other variant be intended to it is non-exclusive
Property includes, so that process, method, article or equipment including a series of elements not only include those key elements, and
Also include the other element that is not expressly set out, or also include for this process, method, article or equipment inherently
Key element.
Claims (5)
- A kind of 1. preparation method of bacillus subtilis liquid fermentation feed, it is characterised in that including：（1）, bacillus subtilis is inoculated into bacillus subtilis bacterium culture medium and cultivated 24~30 hours at 28~30 DEG C, make Into fermentation seed liquid, bacillus subtilis bacterium culture medium includes according to parts by weight：2~3 parts of beef extract；8~10 parts of peptone；Chlorination 4~5 parts of sodium；0.1~0.2 part of potassium dihydrogen phosphate；0.03~0.06 part of magnesium sulfate；18~20 parts of agar；1000 parts of water；（2）, by basal feed and water be according to ratio of water to material（2.5~3）：1 liquid feed inoculation fermentation seed liquor, is enclosed in close Anaerobic fermentation is carried out in envelope container.
- 2. the preparation method of bacillus subtilis liquid fermentation feed according to claim 1, it is characterised in that：Raise on basis Material includes according to weight ratio：Corn 60~65%；Dregs of beans 19~22%；Wheat bran 7~8%；Fish meal 3~4%Surplus is vitamin and trace element.
- 3. the preparation method of bacillus subtilis liquid fermentation feed according to claim 1, it is characterised in that：The hair The inoculum concentration of ferment seed liquor is 3%.
- 4. the preparation method of bacillus subtilis liquid fermentation feed according to claim 1, it is characterised in that：Anaerobism is sent out 60 hours ferment time, 30 DEG C of fermentation temperature.
- 5. the preparation method of bacillus subtilis liquid fermentation feed according to claim 1, it is characterised in that：It is described withered The pH value of careless bacillus culture medium is 7.0~7.2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610421452.6A CN107494889A (en) | 2016-06-14 | 2016-06-14 | The preparation method of bacillus subtilis liquid fermentation feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610421452.6A CN107494889A (en) | 2016-06-14 | 2016-06-14 | The preparation method of bacillus subtilis liquid fermentation feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107494889A true CN107494889A (en) | 2017-12-22 |
Family
ID=60679101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610421452.6A Withdrawn CN107494889A (en) | 2016-06-14 | 2016-06-14 | The preparation method of bacillus subtilis liquid fermentation feed |
Country Status (1)
| Country | Link |
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| CN (1) | CN107494889A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112458017A (en) * | 2020-12-01 | 2021-03-09 | 鹤山市新的生物制品有限公司 | Bacillus subtilis XDK06 and application thereof |
| CN113080308A (en) * | 2021-05-17 | 2021-07-09 | 黄石市佳兴生物科技有限公司 | Fermented fluid milk substitute feed and preparation method thereof |
-
2016
- 2016-06-14 CN CN201610421452.6A patent/CN107494889A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112458017A (en) * | 2020-12-01 | 2021-03-09 | 鹤山市新的生物制品有限公司 | Bacillus subtilis XDK06 and application thereof |
| CN112458017B (en) * | 2020-12-01 | 2022-08-09 | 鹤山市新的生物制品有限公司 | Bacillus subtilis XDK06 and application thereof |
| CN113080308A (en) * | 2021-05-17 | 2021-07-09 | 黄石市佳兴生物科技有限公司 | Fermented fluid milk substitute feed and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171222 |
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| WW01 | Invention patent application withdrawn after publication |