CN107446866A - A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement - Google Patents

A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement Download PDF

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Publication number
CN107446866A
CN107446866A CN201710889926.4A CN201710889926A CN107446866A CN 107446866 A CN107446866 A CN 107446866A CN 201710889926 A CN201710889926 A CN 201710889926A CN 107446866 A CN107446866 A CN 107446866A
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bacillus
excrement
fermentation
geobacillus
dystopy
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戴文霄
叶鼎承
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Fujian Nongke Agriculture Development Co Ltd
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Fujian Nongke Agriculture Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00Housing animals; Equipment therefor
    • A01K1/015Floor coverings, e.g. bedding-down sheets ; Stable floors
    • A01K1/0152Litter
    • A01K1/0155Litter comprising organic material

Abstract

Present invention relates particularly to the composite fermentation microbial inoculum in a kind of livestock and poultry feces dystopy fermentation process system.The microorganism dystopy fermentation process composite bacteria agent of the present invention is made up of following bacterial strain:Compost bacillus (Geobacillus toebii), stearothermophilus ground bacillus (Geobacillus stearothermophilus), pale air bacillus (Aeribacillus pallidus), excrement Psychrobacter (Psychrobacter faecalis), hot denitrogenation ground bacillus (Geobacillus thermodenitrificans) on the spot.The composite bacteria agent of the present invention applies in dystopy fermentation bed, has the advantages that cold-starting is rapid, fermentation temperature is high, the dirty decomposition of excrement is fast, moisture evaporation amount is big, improves the shortcomings that existing microbial inoculum excrement dirt decomposition efficiency is low, bedding and padding consumption is fast.

Description

A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement
Technical field
Present invention relates particularly to the composite fermentation microbial inoculum in a kind of livestock and poultry feces dystopy fermentation process system, belong to cultivation excrement Dirty field of Environment Protection.
Background technology
With livestock structural adjustment and the propulsion of agriculture industrialization, scale, intensive livestock and poultry breeding industry obtain fast Violent development, livestock breeding industry have become one of important pillar industry of livehood economy.But because livestock and poultry breeding industry management is thin The hysteresis of weak, environmental protection concept missing and Environmental Protection in China technology, cause substantial amounts of Animal manure loss thing arbitrarily discharge and loss, Serious pollution is caused to water body, soil, air etc., brings huge hidden danger to living standard of urban and rural population and physical and mental health, simultaneously Also govern the development of livestock and poultry breeding industry itself.
China is livestock and poultry cultivation big country, and 3,800,000,000 tons of livestock and poultry feces are about produced every year in China, is had become dirty more than industry Contaminate the first big pollution sources.2010《National first time Pollutant source investigation publication》It has been shown that, the COD of livestock and poultry breeding industry discharge reach 1268.26 ten thousand tons, account for the 96% of agricultural sources total emission volumn;Total nitrogen and total phosphorus emission are 102.48 ten thousand tons and 16.04 ten thousand tons, point The 38% and 56% of agricultural sources total emission volumn is not accounted for.Livestock and poultry feces turn into the main source of agricultural non-point source pollution.
Livestock and poultry feces then profit, abandon, do harm to.Large-scale livestock and poultry feces, adverse effect is brought to environment and resident living, To be huge resource but if changing excrement as treasured.Therefore it is dirty urgently to work out new, the environmentally friendly excrement for being applicable to China's cultivation cultivation Treatment technology.
Microorganism dystopy fermentation cultivation treatment for cow manure technology is that an item collection excrement dirt minimizing, innoxious and recycling are The complex art of one.Its principle is condition of the aerobic microbiological strain in certain temperature, humidity, carbon-nitrogen ratio and oxygen content Under, mainly using excrement dirt matter as nutriment, the rapid organic matter bred and largely decomposed in excrement dirt and bedding and padding is translated into Humus material, and a large amount of heat energy are discharged simultaneously.
Microorganism dystopy ferments treatment for cow manure technology by Technology Evolutions such as in-situ fermentation cultural technique and aerobic composts, Its technical process is:The excrement dirt in source is collected by the dirty separation of rain, fecaluria, adjusts the dirty fermentation of homogeneous, excrement, terminal in column home Excrement dirt bedding and padding Fertilizer Transformed, realize excrement dirt zero-emission, the treatment for cow manure new technology of recycling.Its product decomposed is mainly water, two Carbonoxide and a small amount of ammonia.
For the characteristic of in-situ fermentation bed, existing many scientific research institutions go out special microorganism microbial inoculum with Corporation R & D.Currently Dystopy fermentative microorganism strain mainly contains the composition such as bacillus subtilis, Actinobacillus and saccharomycete on the market, but at present still Without the special microbial bacterial agent of dystopy microbial fermentation bed, Some Enterprises and raiser are by microbial inoculum or aerobic used in in-situ fermentation bed Compost bacterium is applied on dystopy microbial fermentation bed, following defect be present:
(1) attenuation degree is low, and excrement dirt consumption amount is smaller
Microbial fermentation bed in situ is used in cultural technique, so to consider to cultivate the life comfortableness of livestock and poultry, needs Specially control fermentation bed tempertaure, it is set not enter hot fermentation state, and dystopy is fermented for cultivation treatment for cow manure technology, it is necessary to long The condition of high temperature is under time state, the worm in the dirt of consumption excrement and high temperature killing excrement dirt is decomposed under the microorganism fungus kind condition of high temperature The materials such as ovum, Escherichia coli, therefore in-situ fermentation strain is not particularly suited in dystopy fermentation process.It is and normal in the dystopy fermentation middle and later periods Because original strain non-refractory on the market causes the dirty fermentation of excrement not thorough, hydrogen sulfide and big can be produced in dystopy fermentation house The gases frowzy such as ammonia are measured, dystopy microbial fermentation can eliminate the intrinsic stink of feces of farm processing well and ask Topic.
(2) cellulose-decomposing ability is strong, and bedding and padding consumption is fast
It can grown to improve fermentation bed compared with the startup ability under low ambient temperature and in the high-temperature time section duration, Generally select one of the composition of the strain (such as CN200910180285.0, CN201010224785.2) of trichoderma as microbial inoculum. And trichoderma strain has very strong cellulose-decomposing ability, cause bedding and padding main component such as wood chip, husk rich in cellulose It is too fast Deng material consumption, add the cost of actual motion in dystopy fermentation treatment for cow manure.
Existing microorganism dystopy fermentation treatment for cow manure technology is not mature enough, usually can because of manual operation improper, pig manure Urine concentration is too low or other extraneous factors etc. cause temperature is too low can not quickly start fermentation and decompose that excrement is dirty, and start-up course takes It is long.If dealing with improperly, fermentation bed can be caused to enter resting state for a long time, be unable to reach the dirty consumption purpose of excrement and effect.
In the dirty actual operation of existing microorganism dystopy fermentation process excrement, from more for facultative aerobic microbiological, therefore When oxygen concentration is inadequate in dystopy fermentation tank, aerobic fermentation can occur for microorganism fungus kind not thorough in fermentation tank, so as to produce The irritative gas such as substantial amounts of NH3, H2S, indoles, while being had a negative impact to surrounding enviroment, also endanger periphery personnel with Animal is physically and mentally healthy.
The content of the invention
It is an object of the invention to provide the complex micro organism fungicide that a kind of dystopy fermentation process excrement is dirty, existing bacterium is improved The problems such as agent excrement dirt decomposition efficiency is low, bedding and padding consumption is too fast, while reduce fermentation bed moderate stimulation gas content.
What the present invention was realized in:
The dirty complex micro organism fungicide of the dystopy fermentation process excrement of the present invention, is made up of following bacterial strain:Compost gemma on the spot Bacillus (Geobacillus toebii), stearothermophilus bacillus (Geobacillus stearothermophilus), Pale air bacillus (Aeribacillus pallidus), excrement Psychrobacter (Psychrobacter faecalis), heat Denitrogenation ground bacillus (Geobacillus thermodenitrificans).
Preferably, the mass ratio of each bacterial strain is in the complex micro organism fungicide:Compost bacillus on the spot (Geobacillus toebii) 25%, stearothermophilus ground bacillus (Geobacillus stearothermophilus) 25%th, pale air bacillus (Aeribacillus pallidus) 20-25%, excrement Psychrobacter (Psychrobacter Faecalis) 10%, hot denitrogenation ground bacillus (Geobacillus thermodenitrificans) 15-20%.
Compost bacillus (Geobacillus toebii) is from Chinese industrial Microbiological Culture Collection administrative center on the spot To buy, numbering is CICC 22980, is used as compost high temperature bacterial strain more, can produce thermoase, tolerable to be more than 60 DEG C of high temperature, The protein matter in consumption excrement dirt can be decomposed at high temperature.
On the spot prepared by compost bacillus (Geobacillus toebii):
Slant strains activation, shake-flask seed culture will be passed through by compost bacillus on the spot first;Then fermentation tank training is carried out Support:50~70% that based devices are cumulative volume are cultivated in fermentation tank, inoculum concentration accounts for the 0.1~0.3% of culture volume, fermentation temperature Spend for 60 DEG C, blowing air amount is the ratio 0.9 of 180~200rpm stir cultures, ventilation volume and zymotic fluid:1~1:1, tank pressure 0.03~0.05MPa;Fermentation termination is that gemma number is not less than the 90% of total bacteria count, and the viable count of zymotic fluid is 1.0 × 1010~ 1.5×1010Between cfu/ml;Fermentation tank culture medium each component mass percent is:Peptone 5%, beef leaching thing 3%, NaCl 5%, 5 × 10-3%MnSO4H2O, remaining is ddH2O, pH7.0.
Finally carry out solid absorption:Zymotic fluid wheat bran 1:2~1:3 part by weight absorption, after absorption low temperature dehumidifier do It is dry, crush, powder product content is 2.0 × 109~1.5 × 109Between cfu/ml;
Stearothermophilus ground bacillus (Geobacillus stearothermophilus), from Chinese industrial microorganism bacterium Kind of preservation administrative center is bought, and numbering is CICC 22980, its feature:75 DEG C of maximum growth temperature, minimum growth temperature 30 DEG C, adaptive temperature scope is wide, yielding lipase, can be used for clearing up lipoid material in excrement dirt.
Stearothermophilus bacillus (Geobacillus stearothermophilus) prepare:
First will on the spot stearothermophilus bacillus by slant strains activation, shake-flask seed culture;Then sent out Fermentation tank culture:50~70% that based devices are cumulative volume are cultivated in fermentation tank, inoculum concentration accounts for the 0.1~0.3% of culture volume, Fermentation temperature is 50-55 DEG C, and blowing air amount is the ratio 0.9 of 180~200rpm stir cultures, ventilation volume and zymotic fluid:1~ 1:1, tank presses 0.03~0.05MPa;Fermentation termination is that gemma number is not less than the 90% of total bacteria count, and the viable count of zymotic fluid exists Between 1.0 × 1010~1.5 × 1010cfu/ml;Fermentation tank culture medium each component mass percent is:Peptone 5%, beef Thing 3% is leached, NaCl 5%, 5 × 10-3%MnSO4H2O, remaining is ddH2O, pH7.0.
Finally carry out solid absorption:Zymotic fluid wheat bran 1:2~1:3 part by weight absorption, after absorption low temperature dehumidifier do It is dry, crush, powder product content is 2.0 × 109~1.5 × 109Between cfu/ml;
Pale air bacillus (Aeribacillus pallidus) is from Chinese Research for Industrial Microbial Germ preservation management The heart is bought, numbering CICC22991, can produce alpha-amylase, the starchy material in hydrolyzable cultivation excrement dirt, and energy Enough high temperature resistants.
It is prepared by pale air bacillus (Aeribacillus pallidus):
First by pale air bacillus by slant strains activation, shake-flask seed culture;Then fermentation tank training is carried out Support:50~70% that based devices are cumulative volume are cultivated in fermentation tank, inoculum concentration accounts for the 0.1~0.3% of culture volume, fermentation temperature Spend for 60 DEG C, blowing air amount is the ratio 0.9 of 180~200rpm stir cultures, ventilation volume and zymotic fluid:1~1:1, tank pressure 0.03~0.05MPa;Fermentation termination is that gemma number is not less than the 90% of total bacteria count, and the viable count of zymotic fluid is 1.0 × 1010~ 1.5×1010Between cfu/ml;Fermentation tank culture medium each component mass percent is:Peptone 5%, beef leaching thing 3%, NaCl 5%, 5 × 10-3%MnSO4H2O, remaining is ddH2O, pH7.0.
Finally carry out solid absorption:Zymotic fluid wheat bran 1:2~1:3 part by weight absorption, after absorption low temperature dehumidifier do It is dry, crush, powder product content is 2.0 × 109~1.5 × 109Between cfu/ml;
Excrement Psychrobacter (Psychrobacter faecalis), purchased from Chinese industrial Microbiological Culture Collection administrative center , numbering is CICC 23896, the microorganism fungus kind quickly started in being fermented as dystopy, can accelerate microorganism in room temperature Fermentation starting process.
It is prepared by excrement Psychrobacter (Psychrobacter faecalis):
First by excrement Psychrobacter by slant strains activation, shake-flask seed culture;Then fermentation tank culture is carried out:Fermentation 50~70% that based devices are cumulative volume are cultivated in tank, inoculum concentration accounts for the 0.1~0.3% of culture volume, fermentation temperature 36 DEG C, blowing air amount is the ratio 0.9 of 180~200rpm stir cultures, ventilation volume and zymotic fluid:1~1:1, tank pressure 0.03~ 0.05MPa;Fermentation termination is that gemma number is not less than the 90% of total bacteria count, and the viable count of zymotic fluid 1.0 × 1010~1.5 × Between 1010cfu/ml;Fermentation tank culture medium each component mass percent is:Peptone 15%, soya peptone 5%, NaCl 5%, Remaining is ddH2O, pH7.3.
Finally carry out solid absorption:Zymotic fluid wheat bran 1:2~1:3 part by weight absorption, after absorption low temperature dehumidifier do It is dry, crush, powder product content is 2.0 × 109~1.5 × 109Between cfu/ml;
Hot denitrogenation ground bacillus (Geobacillus thermodenitrificans), aerobic strain, from Chinese industrial Microbiological Culture Collection administrative center buys, and numbering is CICC 22979,60 DEG C of cultures of high temperature, produces thermoase, acid-resisting ring Border, the pH value range of pH growths is 2.5~6.5.
Hot denitrogenation bacillus (Geobacillus thermodenitrificans) prepare:
By hot denitrogenation bacillus passes through slant strains activation, shake-flask seed culture first;Then fermentation tank training is carried out Support:50~70% that based devices are cumulative volume are cultivated in fermentation tank, inoculum concentration accounts for the 0.1~0.3% of culture volume, fermentation temperature Spend for 60 DEG C, blowing air amount is the ratio 0.9 of 180~200rpm stir cultures, ventilation volume and zymotic fluid:1~1:1, tank pressure 0.03~0.05MPa;Fermentation termination is that gemma number is not less than the 90% of total bacteria count, and the viable count of zymotic fluid is 1.0 × 1010~ 1.5×1010Between cfu/ml;Fermentation tank culture medium each component mass percent is:Peptone 5%, beef leaching thing 3%, NaCl 5%, 5 × 10-3%MnSO4H2O, remaining is ddH2O, pH7.0.
Finally carry out solid absorption:Zymotic fluid wheat bran 1:2~1:3 part by weight absorption, after absorption low temperature dehumidifier do It is dry, crush, powder product content is 2.0 × 109~1.5 × 109Between cfu/ml.
The invention has the advantages that:
The present invention in view of the shortcomings of the prior art and livestock and poultry feces main matter composition, from mutually not antagonism, and energy The organic principles such as the rapid albumen decomposed in excrement dirt, fat, starch, while to weaker micro- of cellulose, lignin capacity of decomposition Biological inoculum, composite bacteria agent is prepared into, applied in dystopy fermentation bed, have startup is rapid, fermentation temperature is high, excrement is dirty to decompose It hurry up, the advantages that moisture evaporation amount is big, improve the problem of existing microbial inoculum excrement dirt decomposition efficiency is low, bedding and padding consumption is fast, while this hair Bright composite bacteria agent can consume the materials such as conversion N-NH3, vulcanization class, reduce fermentation bed moderate stimulation gas content.
Embodiment
Embodiment 1:
Microbial inoculum content in mass ratio carries out mixing and is added in fermentation tank, on the spot compost bacillus 25%, stearothermophilus Ground bacillus 25%, pale air bacillus 25%, excrement Psychrobacter 10%, hot denitrogenation ground bacillus 15%.Proportioning Into complex micro organism fungicide.By complex micro organism fungicide according to 1kg strains/3m3Bedding and padding volume ratio is added to dystopy fermentation bed In, then the addition of single groove microbial inoculum is 69kg, and the volume of daily addition dirty per groove excrement is about 4m3.Wherein this experiment hair Ferment groove construction scale is long 45m, inner width 3.85m, the high 1.7m of wall, and fermentation tank bedding and padding loading is long 40m, and bedding and padding are highly 1.4m, width 3.85m, overall accumulated amount is about 207m3.
Measurement result:Fermentation tank temperature in 20 days is observed with reference to table 1, as a result shows in embodiment 1 and adds complex microorganism After adding excrement dirt in microbial inoculum and bedding and padding, every groove adds excrement dirty amount about 4m3 daily, and temperature the 2nd day to the 3rd day is again in fermentation tank Fermentation starting temperature (55 DEG C) can be risen to, and daily addition sewage quantity is about 4m3, in observation period 20d in dystopy fermentation house Do not occur explicitly penetrating odor, be not decreased obviously highly without sewage backflow, the bedding and padding of embodiment 1 in backflash, through measurement It is about 0.02m that display, which declines average height, shows that bedding and padding by fast degradation, can not improve the service life of bedding and padding, reduce dystopy The dirty cost of fermentation process excrement (detailed results are referring to table 1).
Embodiment 2:
Embodiment 1 is repeated, embodiment 2 has following difference, is mainly reflected in that composite bacteria agent formula forms having differences property: Compost bacillus 25% on the spot, stearothermophilus ground bacillus 25%, pale air bacillus 20%, excrement Psychrobacter 10%, hot denitrogenation ground bacillus 20%.
Measurement result:Fermentation tank temperature in 20 days is observed with reference to table 1, as a result shows that temperature exists in fermentation tank in embodiment 2 4th to the 5th day temperature can rise to start-up temperature (55 DEG C), measure 55.2 DEG C within the 4th day, equally operated with embodiment 1 Under the conditions of daily addition excrement dirt about 4m3, do not occur explicitly penetrating odor in dystopy fermentation house in observation period 20d, the 10th It starts drops sewage occur to ooze out backflow pool, until it is 0.5m3 to measure within 14 days sewage backflow amount in backflash first, shows Sewage cumulant is 0.5m in backflash in the three day time from the 11st day to the 14th day.Mid cap is put down within 14th day to the 20th day Daily 0.47m3 sewage flow to backflash, and the bedding and padding of embodiment 2 height is not also decreased obviously, and falling head is shown through measurement About 0.02m, the service life in bedding and padding can be improved, operating cost is reduced for the dirty degraded operational efficiency of cultivation excrement.With this Meanwhile though this experiment is probed into it has been found that having percolate to ooze out in backflash, dystopy fermentation bed is still within fermentation Manage in excrement dirt working condition, simply treatment for cow manure ability has weakened, though show that current bedding and padding have absorption outlet capacity and have deficiency, But dystopy fermentation tank is still in fermentation process excrement dirt working condition.(detailed results are referring to table 1).
Comparative example 3:
Embodiment 1 is repeated, comparative example 3 has following difference, is mainly reflected in that composite bacteria agent formula forms having differences property, The complex microbial inoculum that comparative example 3 is selected is " agriculture section No.1 " complex micro organism fungicide, and complex micro organism fungicide composition is withered Careless bacillus (Bacillus subtilis) 25%, Thermoactinomyces (Thermoactinomyces sp.) 25%, heat Denitrogenation ground bacillus (Geobacillus thermodenitrificans) 20%, streptomyces (Streptomyces Sp.) 20%, enterococcus faecalis (Enterococcus faecalis) 5%, Bacillus megatherium (Bacillus megaterium) 5%.
Measurement result:Fermentation tank temperature in 20 days is observed with reference to table 1, as a result temperature exists in fermentation tank in display comparison example 3 Temperature can rise to start-up temperature (55 DEG C) within 4th to the 5th day, and excrement is added daily under conditions of same operation with embodiment 1 Dirt about 4m3, explicitly excitant ammonia taste gas can be experienced in dystopy fermentation house in observation period 20d, about within 15 day time, When dystopy fermentation tank turns over throwing, personnel can experience excitant ammonia taste gas.Started drops sewage occur at the 8th day to ooze out backflow Pond, sewage scale showed that backflow sewage quantity is 0.5m3 in backflash in backflash later in the 14th day within 3 days, from the 10th day to the After 20 days through measurement draw averagely daily about 0.67m3 sewage backflows, the bedding and padding of comparative example 3 highly occur more significantly under Drop trend, it is about 0.05m to show that average height declines through measurement, and higher than the bedding and padding degradation speed of embodiment 1 and 2, bedding and padding consumed Cultivation treatment for cow manure cost can be improved soon (detailed results are referring to table 1).
Although the foregoing describing the embodiment of the present invention, those familiar with the art should manage Solution, the specific embodiment described by us are merely exemplary, rather than for the restriction to the scope of the present invention, are familiar with this The equivalent modification and change that the technical staff in field is made in the spirit according to the present invention, should all cover the present invention's In scope of the claimed protection.
Table 1:Different case study on implementation group thermograph forms

Claims (2)

  1. A kind of 1. dirty complex micro organism fungicide of dystopy fermentation process excrement, it is characterised in that:It is made up of following bacterial strain:Compost on the spot Bacillus (Geobacillus toebii), stearothermophilus ground bacillus (Geobacillus Stearothermophilus), pale air bacillus (Aeribacillus pallidus), excrement Psychrobacter (Psychrobacter faecalis), hot denitrogenation ground bacillus (Geobacillus thermodenitrificans).
  2. 2. the dirty complex micro organism fungicide of dystopy fermentation process excrement according to claim 1, it is characterised in that:It is described compound The mass ratio of each bacterial strain is in microbial bacterial agent:Compost bacillus (Geobacillus toebii) 25%, thermophilic on the spot Fatty ground bacillus (Geobacillus stearothermophilus) 25%, pale air bacillus (Aeribacillus pallidus) 20-25%, excrement Psychrobacter (Psychrobacter faecalis) 10%, hot denitrogenation Ground bacillus (Geobacillus thermodenitrificans) 15-20%.
CN201710889926.4A 2017-09-27 2017-09-27 A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement Pending CN107446866A (en)

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CN110527643A (en) * 2019-06-25 2019-12-03 大连泰丰昌农业科技有限公司 One plant of soil Psychrobacter and its application in agricultural solid waste low temperature aerobic fermentation
CN114736833A (en) * 2022-05-17 2022-07-12 浙江大学 High-temperature-resistant degradation microbial inoculum for household kitchen waste compost and method thereof

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Publication number Priority date Publication date Assignee Title
CN108191471A (en) * 2018-01-26 2018-06-22 中国农业科学院农业环境与可持续发展研究所 A kind of method for handling livestock culture waste
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CN110527643A (en) * 2019-06-25 2019-12-03 大连泰丰昌农业科技有限公司 One plant of soil Psychrobacter and its application in agricultural solid waste low temperature aerobic fermentation
CN114736833A (en) * 2022-05-17 2022-07-12 浙江大学 High-temperature-resistant degradation microbial inoculum for household kitchen waste compost and method thereof
CN114736833B (en) * 2022-05-17 2023-10-20 浙江大学 High-temperature-resistant degradation microbial inoculum for household kitchen waste compost and method thereof

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