CN103570384B - A kind of composite microbiological fertilizer and production method thereof - Google Patents
A kind of composite microbiological fertilizer and production method thereof Download PDFInfo
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- CN103570384B CN103570384B CN201310515179.XA CN201310515179A CN103570384B CN 103570384 B CN103570384 B CN 103570384B CN 201310515179 A CN201310515179 A CN 201310515179A CN 103570384 B CN103570384 B CN 103570384B
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Abstract
The invention discloses a kind of composite microbiological fertilizer and production method thereof, be specifically related to a kind ofly can meet plant to nitrogen, phosphorus, the potassium composite microbiological fertilizer of needs and production method thereof comprehensively.The technical problem solved is that existing microbial fertilizer is used alone an aspect that can only solve plant desired nutritional, and cost is large compared with high, consumption, with the use of, be difficult to again accomplish suitable scale, cause deficiency or the waste of rate of fertilizer application.The present invention is mixed by the fermented product of nitrogen-fixing bacteria, phosphorus bacteria, B. mucilaginocus.Adopt the present invention after such technical scheme, the comprehensive needs of plant to nitrogen, phosphorus, potassium can be met, be conducive to the development of agriculture production, substitute the nitrogen of more than 60%, phosphorus, potassium fertilizer use, can planting cost be reduced, significantly improve output, also can improve quality and the local flavor of grain and vegetables.
Description
Technical field
The present invention relates to bioengineering field, be specifically related to a kind of composite microbiological fertilizer and production method thereof.
Background technology
Existing microbial fertilizer is monistic microbial fertilizer mostly, as azotobacteria fertilizer, become with fungi ancestral primarily of the bacterium with nitrogen fixation, and for example phosphorus bacteria, its function decomposes the organophosphorus in soil, make it to become the phosphorus element being easy to be absorbed by plants, for another example B. mucilaginocus, its function decomposes the potassium compound in soil, make it to become the available potassium being easy to be absorbed and used by plants, these fertilizer play each self-applying being used alone Shi Junneng, part meets plant to nitrogen, phosphorus, the needs of potassium three elements, they can not produce the soil compaction phenomenon that life-time service chemical fertilizer causes, but its deficiency is: be used alone the aspect that above-mentioned three quasi-microorganism fertilizer can only solve plant desired nutritional, and cost is higher, consumption is large, simultaneously by three with the use of, again because being difficult to accomplish suitable scale, and cause deficiency or the waste of rate of fertilizer application, facts have proved of the microbial fertilizer of the single or compound of life-time service, they can only substitute small portion chemical fertilizer.
Summary of the invention
The technical problem to be solved in the present invention is that existing microbial fertilizer is used alone an aspect that can only solve plant desired nutritional, cost compared with high, consumption is large, with the use of, be difficult to again accomplish suitable scale, cause deficiency or the waste of rate of fertilizer application, provide a kind of and can meet plant to nitrogen, phosphorus, the potassium composite microbiological fertilizer of needs and production method thereof comprehensively.
For achieving the above object, the present invention adopts following technical proposals: a kind of composite microbiological fertilizer, and it is mixed by the fermented product of nitrogen-fixing bacteria, phosphorus bacteria, B. mucilaginocus.Described nitrogen-fixing bacteria is bacillus azotobacter, subtilis, and phosphorus bacteria is bacillus megaterium, and B. mucilaginocus is bacillusmusilaginosiengineering.
The weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 23-38 parts, 20-35 parts, 16--28 part, 10-22 parts.
The weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 35 parts, 30 parts, 20 parts, 15 parts.
The weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 30 parts, 28 parts, 22 parts, 16 parts.
Produce the method for composite microbiological fertilizer, it comprises step: 1, select bacterial classification, by the nitrogen-fixing bacteria chosen, have and decompose the phosphorus bacteria of organic and inorganic phosphorus function and the B. mucilaginocus bacterial strain of decomposable asymmetric choice net potassium compound, carry out respectively cultivating, fermenting, 2, the tunning of selected bacterial classification is added the peat composed of rotten mosses, dregs of beans, wheat bran, Semen Maydis powder, rice meal auxiliary material respectively after ferment again; 3, the product fermented again is combined as composite microbiological fertilizer in proportion.
Described the tunning of selected bacterial classification is added add respectively when auxiliary material ferments again the peat composed of rotten mosses, dregs of beans, wheat bran, Semen Maydis powder, rice meal weight part be respectively 70 parts, 20 parts, 7 parts, 2 parts, 1 part.
Substantially being described below of each bacterium of the present invention:
Bacillus azotobacter is cell direct rod shape, two ends circular or truncate, 0.5-25 × 1.2--10um is paired or catenation usually, tool nose circle or square end, cell dyeing in young age, is Gram-positive during cultivation mostly, sporangiocyst expands, sometimes circular or cylindrical, utilize Citrate trianion, can grow at broth culture.
Subtilis is a kind in bacillus, cell is shaft-like, little chaining, size is 0.7--0.8 × 2--3um, and sporangium is not expanded, raw in gemma ellipse, on nutrient agar, bacterium colony is circular or irregular, surface colour is dark, or is opaque, and wrinkle may be had to become cream-colored or brown.
Bacillus megaterium is a kind in bacillus, shaft-like, end circle, the single or arrangement in short chain, cell size, 1.2-2.0 × 02--5um, brood cell's capsule does not expand, middle life, gemma oval, Gram-positive, aerobic, nutrient agar medium is heaped shape growth, do not expand, the organic and inorganic phosphorus in soil can be decomposed, become the available rapid available phosphorus of plant.
Bacillusmusilaginosiengineering is a kind in bacillus, bacterium colony protuberance in the artificial culture of carbon containing and ammonium nitrogen, translucent, have mucus, edge is smooth, colloid thickness, in gel-shaped diameter 0.5--1.0cm, this substratum can form gemma, phosphorus, the potassium mineral such as silicate and phosphorus lime can be utilized.
Adopt the basic condition that the present invention is directed to China's soil fertility of technique scheme, particularly very this content status of wherein nitrogen, phosphorus, potassium three elements, design the composite microbiological fertilizer containing auxiliary materials such as vinelandii, phosphorus bacteria, B. mucilaginocus and the peats composed of rotten mosses, the comprehensive needs of plant to nitrogen, phosphorus, potassium can be met.Solve current domestic nitrogenous fertilizer (chemical fertilizer) inadequate resource and expensive problem, reduce fertilizer cost, be conducive to the development of agriculture production, the ratio combination determined by orthogonal experiment by two kinds of vinelandii and phosphorus bacteria, B. mucilaginocus forms, therefore the deficiency of the unicity of existing chemical fertilizer and microbial fertilizer can be supplemented, it is applicable to the plant growth in China's Different Soil, substitute the nitrogen of more than 60%, phosphorus, potassium fertilizer use, planting cost can be reduced, significantly improve output, also can improve quality and the local flavor of grain and vegetables.
Embodiment
The present invention includes a kind of composite microbiological fertilizer, it is mixed by the fermented product of nitrogen-fixing bacteria, phosphorus bacteria, B. mucilaginocus.Described nitrogen-fixing bacteria is bacillus azotobacter, subtilis, and phosphorus bacteria is bacillus megaterium, and B. mucilaginocus is bacillusmusilaginosiengineering.
The weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 23-38 parts, 20-35 parts, 16--28 part, 10-22 parts.
The weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 35 parts, 30 parts, 20 parts, 15 parts.
The weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 30 parts, 28 parts, 22 parts, 16 parts.
Produce the method for composite microbiological fertilizer, it comprises step: 1, select bacterial classification, by the nitrogen-fixing bacteria chosen, have and decompose the phosphorus bacteria of organic and inorganic phosphorus function and the B. mucilaginocus bacterial strain of decomposable asymmetric choice net potassium compound, carry out respectively cultivating, fermenting, 2, the tunning of selected bacterial classification is added the peat composed of rotten mosses, dregs of beans, wheat bran, Semen Maydis powder, rice meal auxiliary material respectively after ferment again; 3, the product fermented again is combined as composite microbiological fertilizer in proportion.
Described the tunning of selected bacterial classification is added add respectively when auxiliary material ferments again the peat composed of rotten mosses, dregs of beans, wheat bran, Semen Maydis powder, rice meal weight part be respectively 70 parts, 20 parts, 7 parts, 2 parts, 1 part.
Below for cultivating bacillus azotobacter, subtilis, bacillus megaterium and the bacterial classification of bacillusmusilaginosiengineering and the preparation method of each strain fermentation product:
The cultivation of bacillus azotobacter bacterial classification and the preparation method of strain fermentation product: comprise the following steps: 1, a small amount of bacterial classification is cultivated in test-tube culture medium, the batching of test-tube culture medium is as shown in table 1, and bacillus azotobacter bacterial classification is cultivated 24-48 hour at 30 DEG C.2, the strain inoculation of turning out on test tube base to liquid nutrient medium being turned out liquid spawn (first class inoculum), the batching of cultivating the liquid nutrient medium of the liquid spawn test-tube culture medium used with step 1 is identical, does not just add agar.3, liquid spawn enlarged culturing liquid culture gone out is second class inoculum, and used medium is identical with the liquid nutrient medium of the 2nd step, cultivates in aerobic fermentation tank.4, solid medium is configured, the batching of solid medium is as shown in table 2, add the liquid nutrient medium identical with the 2nd step, the weight of liquid nutrient medium is 50% of this step solid medium, sterilizing 1 hour under 0.14Mpa, less than 40 DEG C are accessed second class inoculum, and inoculum size walks 20%-30% of total substratum total amount for this reason, cultivate 24-48 hour.5, cultivation terminates rear less than 60 degree vacuum-dryings, obtains bacillus azotobacter fermented product.
Table 1: the allocation sheet of bacillus azotobacter test-tube culture medium
Table 2: the allocation sheet of bacillus azotobacter solid medium
The cultivation of Bacillus subtilis strain: comprise the following steps: 1, cultivated in test-tube culture medium by a small amount of bacterial classification, the batching of test-tube culture medium is as shown in table 3, and Bacillus subtilis strain is cultivated 24-48 hour at 30 DEG C.2, the strain inoculation of turning out on test tube base to liquid nutrient medium being turned out liquid spawn (first class inoculum), the batching of cultivating the liquid nutrient medium of the liquid spawn test-tube culture medium used with step 1 is identical, does not just add agar.3, liquid spawn enlarged culturing liquid culture gone out is second class inoculum, and used medium is identical with the liquid nutrient medium of the 2nd step, cultivates in aerobic fermentation tank.4, solid medium is configured, the batching of solid medium is as shown in table 2, add the liquid nutrient medium identical with the 2nd step, the weight of liquid nutrient medium is 50% of this step solid medium, sterilizing 1 hour under 0.14Mpa, less than 40 DEG C are accessed second class inoculum, and inoculum size walks 20%-30% of total substratum total amount for this reason, cultivate 24-48 hour.5, cultivation terminates rear less than 60 degree vacuum-dryings, obtains fermentation of bacillus subtilis thing.
Table 3: the allocation sheet of subtilis test-tube culture medium
The cultivation of bacillus megaterium bacterial classification: comprise the following steps: 1, cultivated in test-tube culture medium by a small amount of bacterial classification, the batching of test-tube culture medium is as shown in table 4, and bacillus megaterium bacterial classification is cultivated 24-48 hour at 30 DEG C.2, the strain inoculation of turning out on test tube base to liquid nutrient medium being turned out liquid spawn (first class inoculum), the batching of cultivating the liquid nutrient medium of the liquid spawn test-tube culture medium used with step 1 is identical, does not just add agar.3, liquid spawn enlarged culturing liquid culture gone out is second class inoculum, and used medium is identical with the liquid nutrient medium of the 2nd step, cultivates in aerobic fermentation tank.4, solid medium is configured, the batching of solid medium is as shown in table 2, add the liquid nutrient medium identical with the 2nd step, the weight of liquid nutrient medium is 50% of this step solid medium, sterilizing 1 hour under 0.14Mpa, less than 40 DEG C are accessed second class inoculum, and inoculum size walks 20%-30% of total substratum total amount for this reason, cultivate 24-48 hour.5, cultivation terminates rear less than 60 degree vacuum-dryings, obtains bacillus megaterium fermented product.
Table 4: the allocation sheet of bacillus megaterium test-tube culture medium
The cultivation of bacillusmusilaginosiengineering bacterial classification: comprise the following steps: 1, cultivated in test-tube culture medium by a small amount of bacterial classification, the batching of test-tube culture medium is as shown in table 5, and bacillusmusilaginosiengineering bacterial classification is cultivated 24-48 hour at 30 DEG C.2, the strain inoculation of turning out on test tube base to liquid nutrient medium being turned out liquid spawn (first class inoculum), the batching of cultivating the liquid nutrient medium of the liquid spawn test-tube culture medium used with step 1 is identical, does not just add agar.3, liquid spawn enlarged culturing liquid culture gone out is second class inoculum, and used medium is identical with the liquid nutrient medium of the 2nd step, cultivates in aerobic fermentation tank.4, solid medium is configured, the batching of solid medium is as shown in table 2, add the liquid nutrient medium identical with the 2nd step, the weight of liquid nutrient medium is 50% of this step solid medium, sterilizing 1 hour under 0.14Mpa, less than 40 DEG C are accessed second class inoculum, and inoculum size walks 20%-30% of total substratum total amount for this reason, cultivate 24-48 hour.5, cultivation terminates rear less than 60 degree vacuum-dryings, obtains bacillusmusilaginosiengineering fermented product.
Table 5: the allocation sheet of bacillusmusilaginosiengineering test-tube culture medium
Ferment again after cultured four kinds of microbial strainss are added the auxiliary materials such as the peat composed of rotten mosses, dregs of beans, wheat bran, Semen Maydis powder, rice meal respectively; Described the tunning of selected bacterial classification is added add respectively when auxiliary material ferments again the peat composed of rotten mosses, dregs of beans, wheat bran, Semen Maydis powder, rice meal weight part be respectively 70 parts, 20 parts, 7 parts, 2 parts, 1 part.By being respectively by weight of the fermented product of the bacillus azotobacter after fermentation again, subtilis, bacillus megaterium and bacillusmusilaginosiengineering: 23-38 parts, 20-35 parts, 16--28 part, 10-22 parts of batchings, be uniformly mixed pack, composite microbiological fertilizer product of the present invention.During use, be preferably spilled in soil as base manure, annual 1--2 time.
Specific embodiments of the invention are as follows:
Embodiment 1: fermented product 35kg, 30kg, 20kg and 15kg of taking bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering respectively, mix, obtain composite microbiological fertilizer.
Embodiment 2: fermented product 30kg, 28kg, 22kg and 16kg of taking bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering respectively, mix, obtain composite microbiological fertilizer.
Embodiment 3: fermented product 23kg, 20kg, 28kg and 22kg of taking bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering respectively, mix, obtain composite microbiological fertilizer.
Embodiment 4: fermented product 38kg, 35kg, 16kg and 10kg of taking bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering respectively, mix, obtain composite microbiological fertilizer.
Embodiment 5: fermented product 34kg, 28kg, 24kg and 18kg of taking bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering respectively, mix, obtain composite microbiological fertilizer.
Claims (4)
1. a composite microbiological fertilizer, it is characterized in that: it is mixed by the fermented product of nitrogen-fixing bacteria, phosphorus bacteria, B. mucilaginocus, described nitrogen-fixing bacteria is bacillus azotobacter, subtilis, phosphorus bacteria is bacillus megaterium, B. mucilaginocus is bacillusmusilaginosiengineering, and the weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 23-38 part, 20-35 part, 16-28 part, 10-22 part; Its preparation method; comprise the following steps: 1, select bacterial classification; by the nitrogen-fixing bacteria chosen; have and decompose the phosphorus bacteria of organic and inorganic phosphorus function and the B. mucilaginocus bacterial strain of decomposable asymmetric choice net potassium compound; carry out respectively cultivating, fermenting, 2, the tunning of selected bacterial classification is added the peat composed of rotten mosses, dregs of beans, wheat bran, Semen Maydis powder, rice meal auxiliary material respectively after ferment again; 3, the tunning fermented again is combined as composite microbiological fertilizer in proportion.
2. composite microbiological fertilizer according to claim 1, is characterized in that: the weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 35 parts, 30 parts, 20 parts, 15 parts.
3. composite microbiological fertilizer according to claim 1, is characterized in that: the weight part of the fermented product of described bacillus azotobacter, subtilis, bacillus megaterium and bacillusmusilaginosiengineering is respectively: 30 parts, 28 parts, 22 parts, 16 parts.
4. composite microbiological fertilizer according to claim 1, is characterized in that: the tunning of selected bacterial classification is added add respectively when auxiliary material ferments again the peat composed of rotten mosses, dregs of beans, wheat bran, Semen Maydis powder, rice meal weight part be respectively 70 parts, 20 parts, 7 parts, 2 parts, 1 part.
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| CN105084996A (en) * | 2014-05-14 | 2015-11-25 | 黄庆禄 | Preparation and field operation method of ecological planting targeted fertilization drug |
| CN104322242A (en) * | 2014-09-16 | 2015-02-04 | 郎溪庆林生态特色农业观光园有限公司 | Cutting seedling raising method for promoting growth of yellow tea by using microorganisms |
| CN106565285A (en) * | 2016-10-28 | 2017-04-19 | 贵州长康农业生态科技有限公司 | Nutrient solution for rubus idaeusl |
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| CN1970506A (en) * | 2006-12-07 | 2007-05-30 | 张丽华 | Multielement compound microbe bacterial fertilizer |
| CN101538173A (en) * | 2009-05-08 | 2009-09-23 | 北京化工大学 | Method for producing microbial compound bacterial fertilizer |
| CN101851123A (en) * | 2010-05-26 | 2010-10-06 | 王金玲 | High-concentration compound microorganism bacterial fertilizer and production method thereof |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134408A (en) * | 1995-04-28 | 1996-10-30 | 王厚德 | Composite microbial fertilizer |
| CN1654435A (en) * | 2004-12-25 | 2005-08-17 | 南通宏通生物科技有限公司 | Composite microbial manure and method for production thereof |
| CN1904035A (en) * | 2006-05-17 | 2007-01-31 | 新疆惠森生物技术有限公司 | Combined nitrogen fixing phosphorus disintegrating potassium disintegrating micro organism composite fertilizer |
| CN1970506A (en) * | 2006-12-07 | 2007-05-30 | 张丽华 | Multielement compound microbe bacterial fertilizer |
| CN101538173A (en) * | 2009-05-08 | 2009-09-23 | 北京化工大学 | Method for producing microbial compound bacterial fertilizer |
| CN101851123A (en) * | 2010-05-26 | 2010-10-06 | 王金玲 | High-concentration compound microorganism bacterial fertilizer and production method thereof |
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