CN106399193A - Microbial inoculants used for low temperature fermentation of organic fertilizers, and preparation method and application thereof - Google Patents
Microbial inoculants used for low temperature fermentation of organic fertilizers, and preparation method and application thereof Download PDFInfo
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- CN106399193A CN106399193A CN201610945280.2A CN201610945280A CN106399193A CN 106399193 A CN106399193 A CN 106399193A CN 201610945280 A CN201610945280 A CN 201610945280A CN 106399193 A CN106399193 A CN 106399193A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The purpose of the invention is to provide a microbial inoculant used for low temperature fermentation of organic fertilizers. The microbial inoculant comprises Bacillus subtilis WEI-62, Bacillus licheniformis WEI-67, Bacillus amyloliquefaciens WEI-79, Enterococcus faecium WEI-89 and Candida utilis WEI-107, wherein the total quantity of Bacillus subtilis is 1.26 * 10<9> CFU/mL, the total quantity of Bacillus spp is 2.93 * 10<8> CFU/mL, and the total quantity of yeast is 5.74 * 10<8> CFU/mL. Compost adopting livestock manure ad a main raw material is inoculated with 1-5% of the microbial inoculants at -30 ~ -10 DEG C, and heating, fermentation, cooling and decomposition processes of the compost are successfully realized a period of time later in order to prepare the high-quality organic fertilizer. The composite microbial inoculant has the characteristics of fast fermentation heating, long high temperature keeping time and secondary heating under cold region extreme cold winter conditions, and is mainly suitable for large-scale livestock culture farms for processing manure under extreme cold conditions in the north region to produce the organic fertilizer.
Description
Technical field：
The present invention relates to a kind of microbial bacterial agent, particularly to a kind of microbial bacterial agent of cold fermentation fertilizer and its preparation side
Method and application
Background technology：
In China, cultivate the pain spot that the dirty process of excrement is always cultivation industry, govern whole industry value chain, near
The reinforcement of 2 years environmental protection dynamics, treatment for cow manure problem has increased to the height determining whether can be closed down, solution to breeding enterprise
Certainly treatment for cow manure problem is very urgent.Produce fertilizer using microbial fermentation technology, the dirty environmental pollution of excrement can not only be solved
Problem, moreover it is possible to provide green high quality fertilizer for growing industry, is to achieve many things at one stroke.
Using microbial fermentation technology produce fertilizer, need preference temperature and aerobic under the conditions of utilize high-effective microorganism
Carrying out the fermentation of the organic wastes such as excrement dirt, thus producing organic matter, discharging the nutrients such as N P and K simultaneously.In compost mistake
Whether successfully whether heat production in journey, be organic fertilizer fermentation important symbol, but in northern China refrigerant latitudes, winter be excessively cold
Cold, lead to most of microbe to enter resting state, fermentation process termination, the production of fertilizer is absorbed in dead state, impact
Normal treatment for cow manure process.
The microbial bacterial agent that the present invention uses is isolatable from the compost of frigid zone winter spontaneous fermentation, and through long-term screening and
Domestication, all bacterial strains not only can the organic waste such as efficient-decomposition excrement dirt at low temperature, and livestock and poultry and environment are not appointed
What endangers, and microbial bacterial agent can realize being rapidly heated of organic fertilizer fermentation under -30 ~ -10 DEG C of temperature condition, contracting
The short time of becoming thoroughly decomposed is it is ensured that the normal production of refrigerant latitudes winter fertilizer.
Content of the invention：
（One）Goal of the invention：
It is an object of the invention to provide a kind of be used for refrigerant latitudes, to organic wastes such as livestock and poultry feces under the conditions of winter extreme cold
Thing carries out the microbial bacterial agent of organic fertilizer fermentation.
（Two）Technical scheme：
The purpose of the present invention can be achieved through the following technical solutions：
The present invention provides a kind of microbial bacterial agent for frigid zone cryogenic conditions bottom fermentation fertilizer, consisting of bacillus subtilis
Bacterium WEI-62, bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-79, VREF WEI-89 and the first Candida of product
WEI-107.High in the paper simulated municipal sludge composting process that this five plants of bacterium were delivered according to ACTA Scientiae Circumstantiae the 6th phase of volume 27
The method that the screening of warm flora, identification and degradation effect are provided, in the compost of spontaneous fermentation from cryogenic conditions, by many
Secondary single strain compost experiment, compost experiment after the many bacterial strains under cryogenic conditions are compound, and safety experiment screening obtain.
Described bacterial strain proportion of composing is：Bacillus subtilis：Bacillus licheniformis：Bacillus amyloliquefaciens：VREF：
Produce first Candida=5:3:7:5:1.
Present invention also offers a kind of preparation method of the microbial bacterial agent being applied to cold fermentation fertilizer, including following
Step：
Strain culturing：By bacillus subtilis WEI-62, bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-79, dung
The enterococcus WEI-89 and first Candida WEI-107 of product is inoculated in respective slant medium respectively, wherein VREF
WEI-89 use MRS agar medium, 25 DEG C culture 24 h, by bacillus subtilis WEI-62, bacillus licheniformis WEI-67,
Bacillus amyloliquefaciens WEI-79 uses LB agar medium, and 30 DEG C of culture 24 h produce first Candida WEI-107 and use YPD fine jade
Fat culture medium, 22 DEG C of culture 48 h；
The preparation of microbial bacterial agent：Every plant of bacterium first respectively with inclined plane inoculating to the 500 mL shaking flasks equipped with 200 mL seed culture mediums
Individually cultivate, wherein VREF WEI-89 uses MRS fluid nutrient medium, 22 DEG C of quiescent culture 40 h are by bacillus subtilis
WEI-62, bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-79 use LB fluid nutrient medium, and 22 DEG C, 180 rpm train
Support 20 h, produce first Candida WEI-107 and use YPD fluid nutrient medium, 22 DEG C, 180 rpm cultivate 40 h；Again by bodies such as bacterium solution
Long-pending mixing, makes 1 L seed liquor；Seed liquor is all accessed equipped with 200 L seeding tanks of 160 L fermentation mediums, 37 DEG C,
100 rpm cultivate 20 h, are all inoculated into afterwards equipped with 5 T fermentation tanks of 3 T fermentation mediums, 22 DEG C, 200 rpm cultivate
48 h.
Used in such scheme, fermentation medium is：
Corn flour 10 g, glucose 20 g, peptone 15 g, potassium nitrate 2 g, bitter salt 0.5 g, three are hydrated
Dipotassium hydrogen phosphate 0.2 g, calcium carbonate 1 g, 7.5,121 DEG C of sterilizing 20 min of water 1000 mL, pH.
Present invention also offers a kind of application process in fermentation fertilizer for composite bacteria agent of cold fermentation fertilizer, whole
Individual sweat is in plant's field conduct.
Present invention also offers a kind of preparation method of cold fermentation fertilizer, preparation method is as follows：
Under the conditions of -30 ~ -10 DEG C, by animal wastes with auxiliary material by weight for 2-5:1 mixing, regulation carbon-nitrogen ratio is 20-
30:1, it is concurrently accessed the composite bacteria agent of cold fermentation fertilizer, inoculum concentration, by weight 1-5%, is added water and adjusted with biogas fermentative liquid
Water content is 55-65%, periodic monitoring temperature and water content, and the holding compost water content that adds water is 55-65%, under treating that temperature is obvious
Turning during fall, altogether, after temperature is no longer significantly raised, become thoroughly decomposed 5-10 days prepared fertilizers for turning 2-3 time.
Described auxiliary material is one or more of molasses, stalk and powdered rice hulls.
Described animal wastes are pig manure or chicken manure.
Specific embodiment:
Following instance is to preferably explain the present invention, but the present invention is not restricted to the scope described in following instance.
Embodiment one, the production of microbial bacterial agent
Bacterial strain screening：All bacterial strains in microbial bacterial agent are all isolatable from the organic of spontaneous fermentation under the conditions of -30 ~ -10 DEG C
Fertilizer, the bacterial strain being separated to has carried out multiple single strain, combination of two bacterial strain and many bacterial strains respectively and has compounded to organic fertilizer fermentation effect
Experiment, screen the bacterial strain that can be obviously improved composting efficiency, carried out the endotoxin of bacterium again, hemolytic, cytotoxicity and
Multiple security screening experiment such as generation amine.Final determine consisting of of low temperature composite bacteria agent, bacillus subtilis WEI-62,
Bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-79, VREF WEI-89 and the first Candida WEI-107 of product.
Strain culturing：By bacillus subtilis WEI-62, bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-
79th, the VREF WEI-89 and first Candida WEI-107 of product is inoculated in respective slant medium respectively, wherein dung intestines ball
Bacterium WEI-89 uses MRS agar medium, and 25 DEG C of culture 24 h, by bacillus subtilis WEI-62, bacillus licheniformis WEI-
67th, bacillus amyloliquefaciens WEI-79 uses LB agar medium, and 30 DEG C of culture 24 h produce first Candida WEI-107 and use
YPD Agr culture medium, 22 DEG C of culture 48 h.
The preparation of microbial bacterial agent：Every plant of bacterium is first respectively with inclined plane inoculating to 500 mL equipped with 200 mL seed culture mediums
Shaking flask is individually cultivated, and wherein VREF WEI-89 uses MRS fluid nutrient medium, and 22 DEG C of quiescent culture 40 h are by bacillus subtilis
Bacterium WEI-62, bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-79 use LB fluid nutrient medium, 22 DEG C, 180 rpm
Cultivate 20 h, produce first Candida WEI-107 and use YPD fluid nutrient medium, 22 DEG C, 180 rpm cultivate 40 h.Again by bacterium solution etc.
Volume mixture, makes 1 L seed liquor.Seed liquor is all accessed equipped with 200 L seeding tanks of 160 L fermentation mediums, 37
DEG C, 100 rpm cultivate 20 h, are all inoculated into equipped with 5 T fermentation tanks of 3 T fermentation mediums afterwards, 22 DEG C, 200 rpm
Cultivate 48 h.
Used in such scheme, fermentation medium is：
Corn flour 10 g, glucose 20 g, peptone 15 g, potassium nitrate 2 g, bitter salt 0.5 g, three are hydrated
Dipotassium hydrogen phosphate 0.2 g, calcium carbonate 1 g, 7.5,121 DEG C of sterilizing 20 min of water 1000 mL, pH.
Mentioned microorganism microbial inoculum is it is characterised in that described bacterial strain proportion of composing is：Bacillus subtilis：Lichens gemma bar
Bacterium：Bacillus amyloliquefaciens：VREF：Produce first Candida=5:3:7:5:1.Lactic acid bacteria sum, total spore content and yeast are total
The testing result of number is as shown in table 1：
The testing result of viable count in table 1 microbial bacterial agent
| Testing index | Bacillus sum | Lactic acid bacteria sum | Yeast sum |
| Colony forming single-digit | 3.18×109 | 9.38×108 | 1.74×108 |
Embodiment two, the preparation of 1# fertilizer
Under -30 ~ -10 DEG C of temperature condition, take the stalk of 1000 kg pig manure mixing 200 kg chopping, be concurrently accessed
Microbial bacterial agent 12 L of the present invention, is mixed with forklift, and adjusting water content with biogas fermentative liquid is 63 %.
Experimental group：After starting fermentation, reach a high temperature more than 60 DEG C within the 2nd day, maintain 70 DEG C of left sides all the time to the 8th day temperature
The right side, temperature drop to 50 DEG C in the 9th day, after turning, temperature rises to 70 DEG C again quickly, to temperature drop to 40 DEG C in the 16th day, the
After secondary turning, temperature rises again and is maintained between 50-60 DEG C, and temperature drops to less than 20 DEG C within the 20th day, fermentation ends, rotten
Ripe 10 days, during remain the water content between 55-65 %.
Control group（It is not added with microbial inoculum）：It is up to 30 DEG C about in the 3 days temperature starting, be gradually dropped to 0 DEG C about afterwards.
Embodiment three, the preparation of 2# fertilizer
Under -30 ~ -10 DEG C of temperature condition, take 1000 kg pig manures to mix 200 kg powdered rice hulls, be concurrently accessed this
Bright microbial bacterial agent 12 L, is mixed with forklift, and adjusting water content with biogas fermentative liquid is 60%.
Experimental group：After starting fermentation, reach a high temperature more than 60 DEG C within the 2nd day, to temperature drop to 50 DEG C in the 11st day, turning
Temperature rises to 70 DEG C quickly again afterwards, and to temperature drop to 40 DEG C in the 17th day, after second turning, temperature is maintained at 60 DEG C of left sides
The right side, temperature drops to 20 DEG C within the 23rd day, fermentation ends, becomes thoroughly decomposed 10 days about, during remain water content between 55-65%.
Control group（It is not added with microbial inoculum）：At 30 DEG C, temperature is gradually decreased to less than 0 DEG C to 1st day temperature afterwards.
Example IV, the preparation of 3# fertilizer
Under -30 ~ -10 DEG C of temperature condition, 1000 kg pig manures are taken to mix 150 kg stalks and 100 kg molasses, with
When access low temperature composite bacteria agent 12 L, mixed with forklift, with biogas fermentative liquid adjust water content be 66 %.
Experimental group：After starting fermentation, reach a high temperature more than 60 DEG C within the 1st day, to temperature drop to 50 DEG C in the 15th day, turning
Temperature rises to 70 DEG C again quickly afterwards, and to temperature drop to 20 DEG C in the 22nd day, after second turning, temperature did not rise, and sends out
Ferment terminates, and becomes thoroughly decomposed 10 days about, during remain water content between 55-65%.
Control group（It is not added with microbial inoculum）：The highest temperature reaches 39 DEG C within first 5 days, is subsequently gradually dropped to 0 DEG C about.
Embodiment five, the preparation of 4# fertilizer
Under -30 ~ -10 DEG C of temperature condition, take 1000 kg chicken manures to mix 500 kg powdered rice hulls, be concurrently accessed low temperature
Composite bacteria agent 12 L, is mixed with forklift, and adjusting water content with biogas fermentative liquid is 60 %.
Experimental group：After starting fermentation, reach a high temperature more than 60 DEG C within the 2nd day, to temperature drop to 50 DEG C in the 13rd day, turning
Temperature rises to 70 DEG C quickly again afterwards, and to temperature drop to 40 DEG C in the 18th day, after second turning, temperature is maintained at 60 DEG C of left sides
The right side, temperature drops to 20 DEG C within the 26th day, fermentation ends, becomes thoroughly decomposed 10 days about, during remain water content between 55-65%.
Control group（It is not added with microbial inoculum）：Temperature, at 30 DEG C, reaches 46 DEG C on the 3rd day within 1st day, afterwards temperature be gradually decreased to 0 DEG C with
Under.
The mensure of embodiment six, fertilizer coliform and induced worm egg death rate：
The assay method of coliform and induced worm egg death rate in fertilizer, according to national standard《Excrement colibacillus group in fertilizer
Measure》（GB/T19524.1-2004）And national standard《The mensure of induced worm egg death rate in fertilizer》（GB/T 19524.2-
2004）Execution.In fertilizer, the result of excrement colibacillus group and induced worm egg death rate is as shown in table 2
The measurement result of coliform and induced worm egg death rate in table 2 fertilizer
| Title | Coliform（Individual/g） | Induced worm egg death rate |
| 1# fertilizer | <30 | 100% |
| 1# compares | >1000 | 15% |
| 2# fertilizer | <30 | 100% |
| 2# compares | 438 | 5% |
| 3# fertilizer | 30 | 100% |
| 3# compares | >1000 | 20% |
| 4# fertilizer | <30 | 100% |
| 4# compares | 254 | 20% |
The measurement result of experimental group coliform all in 30/g or following, complies fully with agricultural industry criteria《Organic fertilizer》
（NY 525-2014）Requirement（≤ 100/g）, and control group coliform group count exceeds well over agricultural industry criteria《Fertilizer
Material》（NY 525-2014）Requirement.
The measurement result of experimental group induced worm egg death rate is 100%, complies fully with agricultural industry criteria《Organic fertilizer》
（NY 525-2014）Requirement（≥95%）, and control group induced worm egg death rate highest only reaches 20%, does not meet agricultural industry mark
Accurate《Organic fertilizer》（NY 525-2014）Requirement.
The testing result of embodiment six, fertilizer germination percentage and bud and root length：
Take 20, Lettuce seed, be placed in the culture dish having padded double-layer filter paper, add water 15 mL, soaking at room temperature 24 h will be unnecessary
Water control go out, add 10 mL fertilizer leaching liquors, move in 32 DEG C of incubators and cultivate 48 h, will frequent moisturizing in incubation
In order to avoid filter paper is evaporated, each sample set 3 parallel, the 48th h statistics germination percentages, the length of bud and root, result such as table 3, table 4,
Shown in table 5.
* the preparation of fertilizer leaching liquor：Weigh 5 g fertilizers, add in 100 mL water, after shaken well, stand 1 h, then
With three layers of filtered through gauze, this leaching liquor matching while using.
The impact to germination percentage for table 3 fertilizer
| Germination percentage | 1# | 2# | 3# | 4# | Mean value |
| Experimental group | 95% | 90% | 90% | 95% | 92.5% |
| Control group | 30% | 45% | 25% | 25% | 31.25% |
The impact to bud length for table 4 fertilizer（mm）
| Bud length | 1# | 2# | 3# | 4# | Mean value |
| Experimental group | 54.29 | 49.50 | 51.24 | 50.13 | 51.29 |
| Control group | 26.14 | 10.23 | 21.48 | 30.22 | 22.02 |
The impact to root length for table 5 fertilizer（mm）
| Root length | 1# | 2# | 3# | 4# | Mean value |
| Experimental group | 39.11 | 30.17 | 41.55 | 37.26 | 37.02 |
| Control group | 18.44 | 26.17 | 20.80 | 27.35 | 23.19 |
Experimental group germination percentage is higher by nearly 60% than control group, the length of bud 32.4 mm more than control group, and the length of root compares control group
Many 15.14 mm are it is seen that after the low temperature composite bacteria agent of the access present invention, just also can guarantee that fertilizer in the winter of extreme cold
Often ferment, fertilizer fertilizer efficiency is strong, and the roundworm egg that coliform also can drop in level of security, and excrement is thoroughly killed.
Claims (8)
1. a kind of microbial bacterial agent of cold fermentation fertilizer is it is characterised in that comprise following bacterial strain：Bacillus subtilis WEI-
62nd, bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-79, VREF WEI-89 and the first Candida WEI- of product
107.
2. microbial bacterial agent according to claim 1 is it is characterised in that described bacterial strain proportion of composing is：Bacillus subtilis
Bacterium：Bacillus licheniformis：Bacillus amyloliquefaciens：VREF：Produce first Candida：=5:3:7:5:1.
3. a kind of preparation method of the composite bacteria agent of cold fermentation fertilizer is it is characterised in that comprise the following steps：
Strain culturing：By bacillus subtilis WEI-62, bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-79, dung
The enterococcus WEI-89 and first Candida WEI-107 of product is inoculated in respective slant medium respectively, wherein VREF
WEI-89 use MRS agar medium, 25 DEG C culture 24 h, by bacillus subtilis WEI-62, bacillus licheniformis WEI-67,
Bacillus amyloliquefaciens WEI-79 uses LB agar medium, and 30 DEG C of culture 24 h produce first Candida WEI-107 and use YPD fine jade
Fat culture medium, 22 DEG C of culture 48 h；
The preparation of microbial bacterial agent：Every plant of bacterium first respectively with inclined plane inoculating to the 500 mL shaking flasks equipped with 200 mL seed culture mediums
Individually cultivate, wherein VREF WEI-89 uses MRS fluid nutrient medium, 22 DEG C of quiescent culture 40 h are by bacillus subtilis
WEI-62, bacillus licheniformis WEI-67, bacillus amyloliquefaciens WEI-79 use LB fluid nutrient medium, and 22 DEG C, 180 rpm train
Support 20 h, produce first Candida WEI-107 and use YPD fluid nutrient medium, 22 DEG C, 180 rpm cultivate 40 h；Again by bodies such as bacterium solution
Long-pending mixing, makes 1 L seed liquor；Seed liquor is all accessed equipped with 200 L seeding tanks of 160 L fermentation mediums, 37 DEG C,
100 rpm cultivate 20 h, are all inoculated into afterwards equipped with 5 T fermentation tanks of 3 T fermentation mediums, 22 DEG C, 200 rpm cultivate
48 h.
4. preparation method according to claim 3 is it is characterised in that described fermentation medium components are：Corn flour 10
G, glucose 20 g, peptone 15 g, potassium nitrate 2 g, bitter salt 0.5 g, three hypophosphite monohydrate hydrogen dipotassium 0.2
G, calcium carbonate 1 g, 7.5,121 DEG C of sterilizing 20 min of water 1000 mL, pH.
5. a kind of application in fermentation fertilizer for composite bacteria agent of cold fermentation fertilizer was it is characterised in that entirely fermented
Journey is in plant's field conduct.
6. a kind of preparation method of cold fermentation fertilizer is it is characterised in that preparation method is as follows：In -30 ~ -10 DEG C of bars
Under part, by animal wastes with auxiliary material by weight for 2-5:1 mixing, regulation carbon-nitrogen ratio is 20-30:1, it is concurrently accessed cold fermentation
The composite bacteria agent of fertilizer, by weight 1-5%, adding water and adjusting water content with biogas fermentative liquid is 55-65% to inoculum concentration, periodically supervises
Testing temperature and water content, and the holding compost water content that adds water is 55-65%, turning when temperature is decreased obviously, turning 2-3 time altogether
After temperature is no longer significantly raised, becomes thoroughly decomposed 5-10 days and fertilizer is obtained.
7. preparation method according to claim 6 is it is characterised in that described auxiliary material is the one of molasses, stalk and powdered rice hulls
Plant or two or more.
8. preparation method according to claim 6 is it is characterised in that described animal wastes are pig manure, chicken manure.
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| CN110257300A (en) * | 2019-07-16 | 2019-09-20 | 广西富凤农牧集团有限公司 | A kind of chicken manure fermenting agent |
| CN111423994A (en) * | 2020-03-24 | 2020-07-17 | 北京北农企业管理有限公司 | Microbial inoculum combination applied to livestock and poultry manure fermentation and preparation and use methods thereof |
| CN111517841A (en) * | 2020-06-18 | 2020-08-11 | 河南农之宝农业科技有限公司 | Organic material decomposing agent and decomposing method |
| CN112195136A (en) * | 2020-11-06 | 2021-01-08 | 呼伦贝尔学院 | Microbial agent for quickly warming compost at low temperature |
| CN115594548A (en) * | 2022-08-30 | 2023-01-13 | 广东省科学院生物与医学工程研究所（Cn） | Method for biologically converting waste feather into organic fertilizer and application |
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| CN106978366A (en) * | 2017-03-24 | 2017-07-25 | 广西大学 | A kind of mix bacterium agent and its application in compost maturity is promoted |
| CN106978366B (en) * | 2017-03-24 | 2020-07-28 | 广西大学 | Mixed microbial inoculum and application thereof in promoting compost maturity |
| CN106977250A (en) * | 2017-03-28 | 2017-07-25 | 惠州市牧兴农业科技有限公司 | A kind of microbial premix and organic compost |
| CN107500811A (en) * | 2017-08-30 | 2017-12-22 | 河南师范大学 | A kind of preparation method of glutinous rehmannia root swelling tailored version microbial-bacterial fertilizer |
| CN109055262A (en) * | 2018-08-10 | 2018-12-21 | 江苏思威博生物科技有限公司 | A kind of complex micro organism fungicide and preparation method thereof for feces of livestock and poultry low temperature compost fermentation |
| CN110194694A (en) * | 2019-03-08 | 2019-09-03 | 大连理工大学 | The dedicated anti-withered biological organic fertilizer of strawberry and preparation method thereof prepared by ermine fox racoon dog waste |
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| CN110205354A (en) * | 2019-06-24 | 2019-09-06 | 黑龙江奥创生物科技发展有限公司 | A kind of preparation method of earthworm bioactivity fulvic acid |
| CN110257300A (en) * | 2019-07-16 | 2019-09-20 | 广西富凤农牧集团有限公司 | A kind of chicken manure fermenting agent |
| CN111423994A (en) * | 2020-03-24 | 2020-07-17 | 北京北农企业管理有限公司 | Microbial inoculum combination applied to livestock and poultry manure fermentation and preparation and use methods thereof |
| CN111423994B (en) * | 2020-03-24 | 2021-03-05 | 北京北农企业管理有限公司 | Microbial inoculum combination applied to livestock and poultry manure fermentation and preparation and use methods thereof |
| CN111517841A (en) * | 2020-06-18 | 2020-08-11 | 河南农之宝农业科技有限公司 | Organic material decomposing agent and decomposing method |
| CN112195136A (en) * | 2020-11-06 | 2021-01-08 | 呼伦贝尔学院 | Microbial agent for quickly warming compost at low temperature |
| CN112195136B (en) * | 2020-11-06 | 2023-03-24 | 呼伦贝尔学院 | Microbial agent for quickly warming compost at low temperature |
| CN115594548A (en) * | 2022-08-30 | 2023-01-13 | 广东省科学院生物与医学工程研究所（Cn） | Method for biologically converting waste feather into organic fertilizer and application |
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