CN113862195B - Wood fiber degrading composite microbial inoculant containing high-temperature bacteria and fungi and application of wood fiber degrading composite microbial inoculant in tail vegetable compost - Google Patents

Wood fiber degrading composite microbial inoculant containing high-temperature bacteria and fungi and application of wood fiber degrading composite microbial inoculant in tail vegetable compost Download PDF

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CN113862195B
CN113862195B CN202111291428.2A CN202111291428A CN113862195B CN 113862195 B CN113862195 B CN 113862195B CN 202111291428 A CN202111291428 A CN 202111291428A CN 113862195 B CN113862195 B CN 113862195B
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沈其荣
李�荣
张嘉伟
吕子健
刘东阳
刘红军
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Nanjing Agricultural University
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Abstract

The invention provides a lignocellulose degrading composite microbial inoculum containing high-temperature bacteria and fungi and application thereof in tail vegetable compost, belonging to the field of biological fertilizers. The composite microbial inoculum contains Brevibacillus reuteri NJAU-N20, geobacillus stearothermophilus NJAU-B5, bacillus NJAU-ND8 and Rhizopus microsporidianus NJAU-F3-2, and the composite microbial inoculum contains 10 9 Bacterial fermentation liquid with CFU/ml or more and 10 9 The fungus spores are uniformly mixed according to the volume ratio of 1:1:1:1. Compared with single-bacteria fermentation liquor and other composite microbial agent fermentation liquor, the composite microbial agent fermentation liquor can effectively promote straw degradation; compared with the unvaccinated control, the inoculated composite microbial inoculum can effectively promote the aerobic composting efficiency of the mixture of the auxiliary materials of the tail vegetable compatibility, and the developed organic fertilizer can effectively promote the field yield of the facility tomatoes.

Description

Wood fiber degrading composite microbial inoculant containing high-temperature bacteria and fungi and application of wood fiber degrading composite microbial inoculant in tail vegetable compost
Technical Field
The invention belongs to the field of high and new agricultural microorganism technology, and discloses a lignocellulose degrading composite microbial agent containing high-temperature bacteria and fungi and application thereof in tail vegetable compost.
Background
The Chinese is a large agricultural country in the world, and is one of countries with large production amount of the tail vegetables, and a large amount of the tail vegetables are produced every year. Currently, the tail vegetables are treated in an unscientific way such as random dumping, landfill, stacking and the like in most areas, so that a series of environmental, economic and social problems are caused. Therefore, effective measures are adopted to carry out resource utilization on agricultural plant source wastes such as the tail vegetables, and the like, so that the problem to be solved is urgent for the sustainable development of the current agriculture.
At present, the main utilization way of the waste of the tail vegetable agriculture is that the tail vegetables are directly returned to the field, and 4 kinds of organic fertilizers are produced by using the tail vegetables as raw materials to produce marsh gas, finish processing to produce feed and aerobic fermentation. The direct returning operation process is simple, but secondary pollution is easy to cause. The tail vegetables can be used as raw materials for producing methane to obtain energy, but the conditions required by producing methane by utilizing the tail vegetables are harsh, the process is complex, and the formed methane slag and methane liquid also need to be treated again. Although the tail vegetables can be used for the production of feed, the amount consumed is very limited. The aerobic composting process can carry out innocent treatment on agricultural plant source wastes such as the tail vegetables and the like through high-temperature fermentation, and converts the agricultural plant source wastes into organic fertilizer, thereby being one of the most effective ways of high-efficiency resource utilization of the tail vegetables.
In recent years, with the development of molecular biology and high-throughput sequencing technology, the succession of microbial communities and the contribution of the succession to composting in the aerobic composting process are gradually discovered. Numerous studies have found that lignocellulose degrading microorganisms are critical for efficient composting. Therefore, it is important to construct a lignocellulosic degrading composite microbial inoculant containing high temperature bacteria and fungi and to study its abnormal effects in the composting of the tail vegetables.
Disclosure of Invention
The invention aims to screen a novel high-efficiency high-temperature lignocellulose degrading bacterial agent combination aiming at agricultural plant source wastes such as tail vegetables and the like, and utilizes in-situ composting to develop a composting composite bacterial agent.
The aim of the invention can be achieved by the following technical scheme:
a wood fiber degrading compound microorganism combination containing high temperature bacteria and fungi comprises Rhizopus (Rhizopus sp.) NJAU-F3-2 with a preservation number of CGMCC No.23067, brevibacillus reus (Bacillus reuszeri) NJAU-N20 with a preservation number of CGMCC No.18020, bacillus sp.) NJAU-ND8 with a preservation number of CGMCC No.16737 and Bacillus stearothermophilus (Bacillus stearothermophilus) B5 with a preservation number of CGMCC No.14935.
The strain NJAU-F3-2 is high-temperature resistant and can grow at a high temperature of 50 ℃; salt tolerance, capable of growing in a medium containing 15% salt; the developmental tree comparison analysis result constructed by the ITS sequence shows that the strain NJAU-F3-2 has the highest homology with the rhizopus microsporidianus Rhizopus microsporus, and the strain NJAU-F3-2 is identified as the rhizopus microsporidianus fungus by combining the developmental tree comparison analysis result constructed by the ITS sequence and the physiological and biochemical characteristics.
The inventor screens high temperature strains growing at 55 ℃, designs 32 combinations of bacterial groups through a broken rod theory, and measures the capability of single bacteria or combined bacteria to degrade wood fibers together with 38 treatments of single bacteria, thus obtaining excellent composite bacterial groups, namely composite bacterial groups containing rhizopus arpium NJAU-F3-2, bacillus NJAU-N20, bacillus NJAU-ND8 and bacillus B5.
The invention relates to application of a microorganism combination in preparation of a wood fiber degradation composite microbial agent.
A wood fiber degrading composite bacterial agent with concentration not lower than 10 9 The rhizopus microsporidianus NJAU-F3-2 bacterial liquid and the concentration of each ml are not lower than 10 9 CFU/ml of bacillus NJAU-N20, bacillus NJAU-ND8 and bacillus B5 are mixed in equal volume ratio; wherein the concentration of each bacterial liquid is the same.
The composite microbial inoculum is characterized in that:
(1) Effective viable count of strainHigh, liquid inoculum is adopted, and the concentration is 10 9 Individual/ml (fungi) or 10 9 CFU/ml (bacteria) or more;
(2) High temperature resistance, and can grow at a high temperature of 55 ℃;
(3) 3 bacteria are identified as bacillus bacteria, 1 fungus is identified as rhizopus fungus, is harmless to crops, and is non-pathogenic to human and animals;
the preparation method of the lignocellulose degradation composite microbial inoculum containing the high-temperature bacteria and fungi comprises the following steps: the strain Brevibacillus reuteri NJAU-N20, bacillus NJAU-ND8 and Geobacillus stearothermophilus NJAU-B5 were inoculated into LB medium, respectively, and cultured at 55℃and 170rpm for 2 days. The strain rhizopus microsporidianus NJAU-F3-2 was inoculated into PDA medium and cultured at 55℃and 170rpm for 2 days. After the culture is finished, the thalli are collected by centrifugation at 4 ℃, and after the thalli are washed, the concentration is regulated by distilled water to ensure that the bacterial concentration is 10 9 CFU/ml or more, fungal spore of 10 9 More than one/ml; and then uniformly mixing the four fermentation liquids according to the volume ratio of 1:1:1:1 to obtain the wood fiber degrading composite microbial inoculum containing the high-temperature bacteria and the fungi.
Wherein the concentration of bacteria was calculated by OD value and the number of spores was counted by the fungus using a hemocytometer.
The invention relates to application of a lignocellulose degrading composite bacterial agent containing high-temperature bacteria and fungi in degrading agricultural plant source waste; the agricultural plant source waste is preferably one or more of straw, cabbage, mushroom residue and wood dust.
The invention relates to application of a lignocellulose degrading composite microbial inoculum containing high-temperature bacteria and fungi in preparing organic fertilizer by taking a tail vegetable as a raw material.
The method for producing the organic fertilizer by adopting the wood fiber degradation composite microbial inoculant comprises the following steps of:
(1) Mixing the raw materials: fresh tail vegetables (waste capsicum, tomato and eggplant plants and fruits), corn stalks and auxiliary materials (mushroom residues and wood chips) are mixed according to a carbon-nitrogen ratio of 25:1, uniformly mixing, adjusting the initial water content to 65-70%, inoculating the wood fiber degradation composite microbial inoculum disclosed by the invention, wherein the inoculum size is 7-10 ml/kg, uniformly mixing the stack materials after inoculation, and then building the stack materials into a strip stack shape, wherein the stack base materials are 2-4 m wide and 2-3 m high, and the length is not limited;
(2) And (3) composting fermentation: after the fermentation base materials are piled in a fermentation shed according to a strip pile, covering Goel film outside the strip pile, fermenting by adopting an airflow film composting mode, wherein the pile is not turned over in the fermentation process, and aerating at the bottom of the fermentation base materials for 20-30 days;
(3) Post-maturation fermentation: and after the composting fermentation is finished, stacking for 6-7 days to obtain the organic fertilizer.
The organic fertilizer prepared by the method of the invention.
As a preferable aspect of the invention, the organic fertilizer contains 18.1g/kg of total nitrogen and P of total phosphorus 2 O 5 9.1g/kg and Total Potassium K 2 O18.5 g/kg; the organic fertilizer is characterized in that the quantity of the Lawsonia solanaceae in the organic fertilizer is reduced to be undetectable after the detection of the Lawsonia solanaceae by adopting a dilution coating technology.
The organic fertilizer disclosed by the invention is applied to promoting the field growth of vegetables and/or improving the yield of the vegetables.
Advantageous effects
The invention provides a lignocellulose degrading composite microbial inoculum containing high-temperature bacteria and fungi, which is used for efficiently converting agricultural plant source wastes such as tail vegetables and the like into organic fertilizers. The microbial composite inoculant contains 4 single bacteria with high-efficiency straw degrading capability, and consists of 3 bacteria and 1 strain of fungi. The secretase of the microorganisms in the process of degrading the straw has complementary functions and obvious synergistic effect, so that the straw tail vegetable is rapidly and fully degraded under the high-temperature condition, the production efficiency of converting the straw tail vegetable into the organic fertilizer is improved, and the economic benefit and the ecological environment benefit of enterprises are improved.
The composite microbial inoculum is used for degrading the straw, the degradation rate of the straw reaches more than 33% after half a month, and the highest degradation rate of the combination of two bacteria is only about 25%, and the highest degradation rate of single bacteria is only about 20%, so that the degradation effect of the composite microbial inoculum is far higher than that of two bacteria and single bacteria.
The screened composite microbial inoculum is used for composting the auxiliary materials of the tail vegetable waste, and after 25d stacking, the composting degree and speed of the stacked material treated by inoculating the composite microbial inoculum are obviously superior to those of the control without adding the microbial inoculum.
Biological material preservation information
NJAU-B5, classified and named as Geobacillus stearothermophilus (Bacillus stearothermophilus), is preserved in China general microbiological culture Collection center (CGMCC) with a preservation address of China academy of sciences of China, including national academy of sciences of China, including North Chen West Lu No.1, of the Korean area of Beijing, and a preservation date of 2017, 11 months and 20 days, and a preservation number of CGMCC No.14935.
NJAU-N20, classified and named Brevibacillus reuteri (Bacillus reuszeri) deposited in China general microbiological culture Collection center with deposited address of China academy of sciences of North Chenxi Lu No.1 and No. 3 of the Korean area of Beijing, with deposited date of 2019, 6 months and 24 days with deposited number of CGMCC No.18020
NJAU-ND8, bacillus sp, is deposited in China general microbiological culture Collection center (CGMCC), and has a deposit address of China academy of sciences of China, including national academy of sciences of North Chen West Lu 1, korea, beijing, and a deposit date of 2018, 11 months, and 12 days, and a deposit number of CGMCC NO.16737.
NJAU-F3-2 is classified and named Rhizopus sp, and is preserved in China general microbiological culture Collection center (CGMCC) with a preservation address of China academy of sciences of China, including North Chen West Lu No.1 and No. 3, of the Korean area of Beijing, with a preservation date of 2021, month 07 and 22, and a preservation number of CGMCC No.23067.
Drawings
FIG. 1 effects of different combinations of flora on straw degradation
FIG. 2 compost temperature variation graph
FIG. 3 variation of carbon to nitrogen ratio during composting
FIG. 4 germination index of pile during composting
FIG. 5 variation of the quantity of L.solanaceae during composting
FIG. 6NJAU-F3-2 developmental tree
Detailed Description
The tested strain is provided by a high-technology research key laboratory for recycling solid organic wastes in Jiangsu province, and 4 strains of bacteria and 4 strains of fungi respectively can grow rapidly at 55 ℃. The 4 fungi are thermomyces lanuginosus NJAU-F4-11, rhizopus microsporum NJAU-F3-2, aspergillus fumigatus NJAU-F2-13 and rhizopus oryzae NJAU-F3-5 respectively numbered A, B, C, D; the 4 bacteria are bacillus NJAU-ND45, bacillus stearothermophilus NJAU-B5, bacillus NJAU-ND8 and bacillus NJAU-N20, and are respectively numbered 1, 2, 3 and 4.
Inoculating fungi into PDA liquid culture medium, and culturing at 55deg.C and 170rpm for 2 days; bacteria were inoculated into LB medium and cultured at 55℃and 170rpm for 2 days. After each bacteria grows well, the OD value of the bacteria is regulated by sterile water, and the spore number of the fungus blood cell counting plate is counted, so that the concentration of each bacterial liquid is 10 9 And (3) setting 1 bacterium, 2 bacterium, 4 bacterium and 8 bacterium combinations according to a broken bar theory and a random block design, wherein the total number of the single bacterium in the combined bacterium is the same as the total number of spores or bacterial colonies contained in single bacterium treatment.
The single strains are numbered A, B, C, D, 1, 2, 3 and 4 respectively; the two strain combinations are AB, A3, A4, AD, B1, B2, BC, C2, C3, CD, D1, D4, 12, 14, 23 and 34 respectively; the combination of the four strains is ABD1, AD12, ABCD, A123, AC23, ACD3, BD24, B134, B234, BC14, CD24 and C134 respectively; the combination of 8 strains was ABCD1234, a total of 38 complex bacterial groups.
Example 1 degradation effects of Single and Complex bacteria on straw
Weighing a certain amount of straw powder, placing the straw powder into a 250ml triangular flask, absorbing all bacterial liquids with the same total amount, adding the bacterial liquids into the triangular flask, uniformly mixing, placing the bacterial liquids into a 55-DEG incubator for culturing, weighing a dried sample after 15 days, taking the straw without inoculating a strain as a reference, and calculating the degradation rate of the straw by a weightless method.
Results and analysis
The effect of different combinations of flora on straw degradation is shown in figure 2. Among all the single bacteria treatments, the degradation efficiency of the No.2 bacteria (the geobacillus stearothermophilus B5) is the highest and 19.37 percent. Of the two bacteria combinations, the degradation rate of the combination of bacteria No.2 and bacteria No. 3 (NJAU-B5+NJAU-ND 8) is the highest and is 24.52%; among the four strain combinations, fungi B, 2, 3 and 4 (NJAU-F3-2+NJAU-B5+NJAU-ND 8+NJAU-N20) had the highest degradation rate of 33.41% and were significantly greater than the two strains and the single treatment. In addition, the combined degradation rate of 8 strains was 35.32%, indicating that the degradation performance was better as the number of strains was not larger. The results show that the composite flora B234 containing NJAU-F3-2+NJAU-B5+NJAU-ND8+NJAU-N20 has the optimal effect on degrading the straw in all flora combinations.
Example 2 Effect of the inoculating Complex microbial inoculant on the composting efficiency of the Mixed auxiliary Material of the Tail vegetable
The experiment totally designs 2 treatments, namely tail vegetables (capsicum, tomato, eggplant and corn) and auxiliary materials (mushroom residues and wood chips) according to a proportion of 25:1, adding a composite microbial inoculum and tail vegetables (capsicum, tomatoes, eggplants and corns) and auxiliary materials (mushroom residues and wood chips) according to a proportion of 25:1, no composite microbial inoculum is added after mixing. The 2 treatments in this test without and with the complex bacterial agents were designated J1 and J2, respectively. The initial water content of each treatment is regulated to 65-70%, the treatment is put into a fermentation tank, the Gobi film is covered, aeration equipment is opened, aeration is carried out at the bottom of a pile body, the pile is not turned over in the fermentation process, and the length and the width of the base material of the pile body are 4 meters, and the height is 3 meters. Measuring temperature in the fermentation process and sampling to measure the change of physical and chemical properties of materials in the composting process.
And (3) measuring the temperature on the same height (50 cm) of the middle part of the pile body at random by using 3 groups of mercury thermometers at 9:00 am and 15:00 pm each day, and taking the average value of the 3 groups of data as the actual temperature of the pile body.
Air-dried and ground samples were filtered through a 100 mesh sieve, and then "soil agrochemistry analysis" written with reference to Bao Shidan: measuring the total carbon content in the sample by adopting a potassium dichromate oxidation-water bath heating method; measuring the content of total nitrogen in the sample by adopting a semi-trace Kelvin method; C/N is the ratio of total carbon to total nitrogen.
The air-dried sample was ground and sieved through a 20 mesh sieve and then mixed with deionized water at a ratio of 1:10 mix (w/v) were stored in 50mL centrifuge tubes, placed on a horizontal shaker and shaken at 170r/min for 30min, and left to stand for 30min before filtration. 10mL of the filtrate was added to petri dishes with filter paper laid thereon, and 10 seeds of Lepidium (Lepidium) were placed in each petri dish, with a blank control of deionized water. The culture dish was placed in a constant temperature incubator at 25℃and was subjected to dark culture for 2 days, and the number of germinated seeds and root length were measured. Each sample was set up in 3 replicates.
Figure RE-RE-GDA0003393186460000061
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Results and analysis
The effect of adding the composite microbial inoculum on the stack temperature during composting is shown in figure 2. As can be seen from the graph, the temperature of each pile body is quickly increased in the heating period, then reaches the high temperature period and is maintained for a period of time, and then is slowly cooled and repeatedly cooled. The temperature rise in J2 treatment is faster than that in J1, the highest temperature in J2 treatment is 64 ℃, and the temperature in J2 treatment in the later composting period is generally higher than that in J1 treatment, so that the high-temperature period can be advanced under the condition of adding the composite microbial inoculum, the fermentation temperature in the high-temperature period can be improved, the composting process can be effectively shortened, and the rotten fermentation of the tail vegetable straw is more thorough.
The effect of adding the composite microbial inoculum on the carbon-nitrogen ratio is shown in figure 3. As the carbon to nitrogen ratio of the pile of the two groups of treatments became lower, the pile tended to stabilize after day 17. In the early stages of composting, the J2 carbon to nitrogen ratio drops faster than treatment J1. When the compost is decomposed, the microbial decomposition movement is basically stopped, and the carbon-nitrogen ratio is gradually stabilized. The initial carbon-nitrogen ratios of the two groups of pile bodies are respectively as follows: 25.17 and 25.06, the carbon-nitrogen ratio at the end of composting is respectively: 19.62 and 17.21, respectively, by 22.06% and 31.32%.
The effect of the addition of the complex inoculant on germination index is shown in FIG. 4. As composting proceeds, the germination index of both treatments shows a growing trend. The germination index of seeds treated with J2 was always higher than J1. The germination indexes of the three treatments are basically the same in growth trend 5 days before composting, the germination indexes are all 50-70%, the growth trend of the treatment J2 is faster than that of the treatment J1 on the 9 th day of composting, and the germination indexes of the seeds are all about 80% at the end of composting; among them, treatments J1 and J2 reached the highest germination index at day 23, which was 80.21% and 90.42%, respectively.
The effect of adding the composite microbial inoculum on the relative amounts of total nitrogen, total phosphorus and total potassium is shown in table 1 below. As can be seen from Table 1, the total N, total P, and total K at the end of composting are all increased for each treatment compared to the composting start-up phase. Treatment J2 all N, all P, all K was 18.1g/kg, 9.1g/kg and 18.5g/kg, respectively, by the end of composting; the total N, total P and total K of the treatment J1 are respectively 17.7g/kg, 8.2g/kg and 17.9g/kg, and the total N, total P and total K of the treatment J2 are the highest.
TABLE 1 variation of nutrient relative content during composting
Figure RE-RE-GDA0003393186460000071
Note that: all results are on a dry weight basis; the different letters represent significant differences at the P <0.05 level.
Example 3 Effect of the Compound inoculant on the quantity of Laurella of Solanaceae during composting of the adjuvant
Selecting a fresh sample of the inoculated composite microbial inoculum stack in example 2 and a fresh sample of the non-inoculated composite microbial inoculum stack in a 250ml triangular flask with deionized water 1: mixing at ratio of 10, oscillating at 30deg.C and 170rpm for 30min, and gradient diluting the shaking suspension with sterile water to obtain 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 And 10 -7 Seven dilution gradients. Laurus solanaceae selection 10 -1 And 10 -2 0.1ml of the diluted solution was spread on SMSA medium, and after spreading, the plates were placed in a 30℃incubator for 2 days and counted.
Results and analysis
The amount of L.solanaceae showed a decreasing trend in the early stage of composting (day 1-9), and in each treated sample, the amount of the strain was decreased to almost 10% on day 1 at day 9, and then maintained at 20X 10 3 ～50×10 3 Between CFU/g (days 9-23). On day 9, the number of L.solani in the J1 treated sample was 35.5X10 3 CFU/g, J2 is 21X 10 3 CFU/g. J2-treated Lawsonia solanaceae numbers by the end of compostingThe amount was not detected.
Example 3 organic fertilizer fermented by inoculating Compound microbial agent on the seed of field tomato
And (3) applying the organic fertilizer fermented by adding or not adding the composite microbial inoculum, researching the influence of the organic fertilizer fermented by inoculating the composite microbial inoculum on the tomato yield, and judging the growth promoting effect of the fertilizer. A total of 5 treatments were set up for the field trial, treatment 1 (CK) respectively: no fertilizer is applied; treatment 2 (CF): applying fertilizer, wherein urea, superphosphate (P) 2 O 5 ) And potassium sulfate (K) 2 The application amount of O) is 210g, 830g and 220g in each cell respectively; treatment 3 (VW): 6.8kg of the tail vegetable raw material is applied, and the rest nutrients are supplemented by chemical fertilizers, so that the total nutrients are equal to those of treatment 2 (CF); treatment 4 (a): 6.8kg of organic fertilizer prepared by fermenting J1 without adding a composite microbial inoculum is applied, and the residual nutrients are supplemented by chemical fertilizers, so that the total nutrients are equal to those of treatment 2 (CF); treatment 5 (B): 6.8kg of organic fertilizer prepared by adding the composite microbial inoculum into J2 and fermenting is applied, and the rest nutrients are supplemented by chemical fertilizers, so that the total nutrients are equal to those of treatment 2 (CF). The yield of each treated tomato fruit was measured at harvest season.
Results and analysis
The tomato yield of different treatments in the field test is shown in table 2, the treatment yield of the organic fertilizer fermented by applying the composite microbial inoculum is 1741.34kg per hectare, the yield is 410.58kg per hectare compared with CK, the yield is 30.88%, the yield is 299.63kg per hectare compared with the tail vegetable raw material, and the yield is 20.78%. The organic fertilizer prepared by adding the composite microbial inoculum for fermentation has the treatment yield of 2166.3kg, and the yield per hectare is increased by 835.81kg compared with CK, and the yield is increased by 62.82%; yield increase 724.59 kg per hectare compared with raw materials, 54.46%, 308.76kg per hectare compared with chemical fertilizer, and 16.62%; compared with the organic fertilizer prepared by fermenting without adding the composite microbial inoculum, the yield per hectare is increased by 424.96kg, and the yield is increased by 24.4%. Therefore, the organic fertilizer prepared by adding the composite microbial inoculum for fermentation has better yield-increasing effect on tomatoes than that of chemical fertilizers and other treatments.
TABLE 2 field trials effects of different treatments on tomato yield
Figure RE-RE-GDA0003393186460000081
Note that: CK, no fertilization treatment; RM, applying the tail vegetable raw material treatment; CF, applying chemical fertilizer for treatment; treatment a, applying J1 to treat compost; treatment B, J2 treatment compost.

Claims (14)

1. A lignocellulose degrading composite microorganism combination containing high-temperature bacteria and fungi is characterized by comprising rhizopus microsporidianus with a preservation number of CGMCC No.23067Rhizopus microsporus) NJAU-F3-2 Brevibacillus reuteri with preservation number of CGMCC NO.18020Brevibacillus reuszeri) Bacillus with preservation number of CGMCC No.16737 and NJAU-N20Bacillussp.) NJAU-ND8 and Bacillus stearothermophilus with a collection number of CGMCC No.14935Geobacillus stearothermophilus) NJAU-B5.
2. A composite microbial agent prepared from the microbial combination of claim 1.
3. The composite microbial agent according to claim 2, wherein the concentration is not less than 10 9 The rhizopus microsporidianus NJAU-F3-2 bacterial liquid and the concentration of each ml are not lower than 10 9 CFU/ml of the bacterial liquid of the Brevibacillus reuteri NJAU-N20, the bacillus NJAU-ND8 and the geobacillus stearothermophilus NJAU-B5 are mixed in equal volume ratio; wherein the concentration of each bacterial liquid is the same.
4. The composite microbial inoculant of claim 3, wherein the composite microbial inoculant is prepared by the following steps: respectively inoculating the Brevibacillus reuteri NJAU-N20, the bacillus NJAU-ND8 and the geobacillus stearothermophilus NJAU-B5 into LB culture media, and culturing for 45-50 h at 50-60 ℃ and 150-180 rpm; inoculating a strain rhizopus microsporidianus NJAU-F3-2 into a PDA culture medium, and culturing for 45-50 hours at 50-60 ℃ and 150-180 rpm; after the culture is finished, the thalli are collected by centrifugation at 4 ℃, and after the thalli are washed, the concentration is regulated by distilled water to ensure that the bacterial concentration is 10 9 CFU/ml or more, fungal sporesAt 10 9 More than one/ml; and then uniformly mixing the four fermentation broths according to the volume ratio of 1:1:1 to obtain the composite microbial inoculum.
5. The use of the composite microbial inoculant of any one of claims 2-4 for degrading agricultural plant source waste.
6. The method according to claim 5, wherein the agricultural plant source waste is one or more of straw, cabbage, mushroom residue and wood chips.
7. The use of the composite microbial inoculant of any one of claims 2-4 for accelerating composting fermentation of agricultural plant source waste.
8. The use according to claim 7, wherein the agricultural plant source waste is one or more of straw, cabbage, mushroom residue, and wood chips.
9. The use of the composite microbial inoculum of any one of claims 2-4 in preparing organic fertilizer by using agricultural plant source waste as raw material.
10. The use according to claim 9, wherein the agricultural plant source waste is one or more of straw, cabbage, mushroom residue, and wood chips.
11. A method for producing an organic fertilizer by using the composite microbial inoculum according to any one of claims 2-4 and agricultural plant source waste as a raw material, which is characterized by comprising the following steps:
(1) Mixing the raw materials: fresh tail vegetables, corn stalks and auxiliary materials are mixed according to a carbon-nitrogen ratio of 25:1, the initial water content is regulated to 65-70%, the composite microbial inoculum according to any one of claims 2-4 is inoculated, the inoculation amount is 7-10 ml/kg, the stack materials are uniformly mixed after inoculation, and then the stack materials are built into a strip stack shape, the width of the stack base material is 2-4 m, the height is 2-3 m, and the length is not limited; the auxiliary materials are mushroom residues and wood chips;
(2) And (3) composting fermentation: after the fermentation base materials are piled in a fermentation shed according to a strip pile, covering Goel film outside the strip pile, fermenting by adopting an airflow film composting mode, wherein the pile is not turned over in the fermentation process, and aerating at the bottom of the fermentation base materials for 20-30 days;
(3) Post-maturation fermentation: and after the composting fermentation is finished, stacking for 6-7 days to obtain the organic fertilizer.
12. An organic fertilizer prepared according to the method of claim 11.
13. The organic fertilizer according to claim 12, wherein the organic fertilizer comprises 18.1g/kg of total nitrogen, 9.1g/kg of total phosphorus and 18.5g/kg of total potassium; the organic fertilizer is characterized in that the quantity of the Lawsonia solanaceae in the organic fertilizer is reduced to be undetectable after the detection of the Lawsonia solanaceae by adopting a dilution coating technology.
14. Use of the organic fertilizer of claim 12 for promoting vegetable field growth and/or increasing vegetable yield.
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