CN114045247B - Bacillus seawater and application thereof in salinized farmland production - Google Patents
Bacillus seawater and application thereof in salinized farmland production Download PDFInfo
- Publication number
- CN114045247B CN114045247B CN202111627771.XA CN202111627771A CN114045247B CN 114045247 B CN114045247 B CN 114045247B CN 202111627771 A CN202111627771 A CN 202111627771A CN 114045247 B CN114045247 B CN 114045247B
- Authority
- CN
- China
- Prior art keywords
- bacillus
- seawater
- cgmcc
- preservation number
- improving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses bacillus seawater and application thereof in salinization farmland production, belonging to the technical field of salt-tolerant microorganisms. The bacillus seawater is bacillus seawater (Bacillus aquimaris) JES4 strain with a preservation number of CGMCC NO.20301. The bacillus maritimus JES4 strain can obviously promote wheat germination, seedling emergence and accumulation of wheat biomass in a salinity environment with the salt concentration of 0.2-0.8%, and has wide application prospect in the agricultural production of salinized soil.
Description
Technical Field
The invention belongs to the technical field of salt-tolerant microorganisms, and particularly relates to bacillus seawater and application thereof in salinized farmland production.
Background
Worldwide, over 8.33 hectares are affected by salinity. The salinized soil has huge area and various varieties, and has very serious influence on agricultural production and ecological environment. In recent years, the method has very important roles in repairing and efficiently utilizing salinized soil, guaranteeing grain safety, providing agricultural yield and optimizing industrial structures. However, in soil salinization, due to the characteristics of high salt content, complex soil structure, low microbial activity and the like, the soil salinization treatment faces serious challenges. Microorganisms are driving forces for the transformation of substances in soil, and the remodeling and regulation by microorganisms are very effective and simple paths for improving the activity of soil samples.
Salinized soil is special in environment and in dynamic change, the ecological environment is fragile, vegetation types are single, species diversity is low, exogenous organic matter input quantity is less, and the salinized soil is also a key factor for limiting the microbial activity of soil. At present, products for improving soil microorganisms are more, but the application range is smaller, and the microbial soil conditioner is difficult to popularize in areas with higher salinity. By supplementing microbial agents, the microbial community structure and the soil property of the soil are improved, the utilization efficiency of salinized soil is improved, and the key is to obtain functional strains which adapt to and improve the salinized soil. At present, the high-efficiency and salt-tolerant functional strains related to the salinized soil are fewer, and the development work for improving the production capacity and benefit of the salinized soil through a microbiological technology is urgent.
Disclosure of Invention
Aiming at the problems existing in the prior art, one of the purposes of the invention is to provide a bacillus seawater which is bacillus seawater (Bacillus aquimaris) JES4 strain with the preservation number of CGMCC NO.20301.
The second purpose of the invention is to provide the application of the bacillus marinus with the preservation number of CGMCC NO.20301 in the production of salinized farmlands, in particular to improve the germination rate and the emergence rate of crops in the salinized farmlands, promote the accumulation of biomass and improve the yield.
The invention further aims to provide a microbial preparation for producing the salinized farmland, which comprises bacillus seawater with the preservation number of CGMCC No.20301, and the microbial preparation is used for improving the germination rate and the emergence rate of crops in the salinized farmland, promoting the accumulation of biomass and improving the yield.
The invention aims at providing a method for improving the germination rate and the emergence rate of crops in salinized farmlands, promoting the accumulation of biomass and improving the yield, in particular to a method for soaking seeds by using fermentation liquor of bacillus marinus with the preservation number of CGMCC No.20301 or inoculating the fermentation liquor of bacillus marinus with the preservation number of CGMCC No.20301 into planting soil.
In a specific embodiment, the bacterial concentration of the fermentation liquid of the seawater bacillus with the preservation number of CGMCC NO.20301 is more than or equal to 2.5x10 9 CFU/mL。
In a specific embodiment, the fermentation broth of bacillus seawater with the preservation number of CGMCC NO.20301 is prepared by the following method: inoculating the lawn preserved on the slant culture medium into LB liquid culture medium, shake culturing at 37deg.C (120 rpm), transferring to new LB liquid culture medium when it reaches logarithmic phase, and culturing under the same conditions until it reaches logarithmic phase to obtain the desired fermentation broth.
In a specific embodiment, the seed soaking is to mix fermentation liquor of bacillus seawater with the preservation number of CGMCC No.20301 with crop seeds to be sown according to the volume-mass ratio of 1:1, and soaking.
In a specific embodiment, the fermentation broth of bacillus seawater with the preservation number of CGMCC No.20301 is inoculated into the planting soil according to the proportion of inoculating 2.5mL of fermentation broth into each 50g of soil and uniformly mixed.
The technical scheme of the invention has the advantages that:
an important mechanism of the bacillus marinus (Bacillus aquimaris) JES4 strain in the invention for promoting wheat growth in salinized soil is to promote the growth of wheat root system and improve the adaptability and tolerance to salt environment.
The bacillus seawater (Bacillus aquimaris) JES4 with the preservation number of CGMCC NO.20301 provided by the invention can obviously promote wheat germination, seedling emergence and accumulation of wheat biomass in a salinity environment with the salt concentration of 0.2-0.8%, and has a wide application prospect in salinized soil agricultural production.
Drawings
FIG. 1JES4 strain colony morphology;
FIG. 2 phylogenetic analysis of 16S rDNA of JES4 strain;
FIG. 3 shows the growth variation of B.seawater (Bacillus aquimaris) JES4 in liquid media of different salinity;
FIG. 4 shows the effect of JES4 strain on wheat germination and emergence rate under salt stress conditions
FIG. 5 is the effect of JES4 strain on wheat seedling stage (14 d) biomass in saline soil;
FIG. 6 is a graph showing the effect of JES4 strain on wheat yield in salinized soil.
Detailed Description
The terms used in the present invention generally have meanings commonly understood by those of ordinary skill in the art unless otherwise indicated.
The invention will be described in further detail below in connection with specific embodiments and with reference to the data. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.
Example 1
Isolation, screening and identification of bacillus seawater (Bacillus aquimaris) JES4
Taking 10g of coastal salinized rhizosphere soil (coastal saline-alkali soil of coastal state), adding the soil with soluble salt content of 0.9% into a triangular flask filled with 100mL of sterile water, oscillating at high speed for 20min, diluting the soil mixture according to gradient, and selecting concentration gradient to be 10 –2 、10 –3 、10 –4 、10 –5 、10 –6 And (3) coating the soil mixture of the strain (2), sucking 100 mu L of bacterial suspension by a pipette, coating the bacterial suspension in an LB solid medium of 8% sodium chloride, selecting single colonies which can grow on a flat plate for separation and purification, screening according to the single colonies which grow on the flat plate, activating and inoculating the screened single colonies into wheat, screening a strain capable of promoting biomass accumulation of the wheat, obtaining a monoclonal strain JES4, and storing the purified strain in a refrigerator at-80 ℃ by using 40% glycerol.
Morphological observation of JES4 strain colonies and cells:
as shown in FIG. 1, the bacterial strain was cultured on S-G plates for 3d with a colony diameter of 2-3 mm, and the colony was round, indicating smooth, low-bulge, opaque, orange-yellow. The thalli are straight rods, are arranged in a chain shape, are formed into flagella, and are gram-positive.
Physiological and biochemical characteristics of JES4 strain:
the growth temperature is as follows: 4-40%, and aerobic NaCl tolerance between 0-11.7%. The contact enzyme, methyl red test and oxidase test are positive, glucose, fructose and mannitol can be used as carbon sources, xylose can not be used, ammonium nitrate and ammonium sulfate can be used, starch and gelatin can be hydrolyzed, and hydrogen sulfide and hydrogen peroxide are not generated.
Determination of the 16S rDNA Gene sequence of JES4 Strain:
1mL of bacterial liquid is collected in the bacterial growth logarithmic phase and is used for extracting genome DNA, and after the genome DNA extraction is finished, the 16S rRNA gene universal primer is utilized for PCR amplification, and the sequencing of the sequence is delivered to Shanghai Biotechnology Co. The 16S rDNA gene sequence of the JES4 strain is shown as SEQ ID NO:1 is shown in the specification; and comparing the sequencing results, and obtaining high-ratio sequences and species through an NCBI (https:// blast. NCBI. Nlm. Nih. Gov/blast. Cgi) database. Phylogenetic analysis was constructed on the obtained sequence based on MEGA 6. The phylogenetic result shows (figure 2) that the sequence length of the JES4 strain 16S rRNA gene is 1449bp, the similarity of the sequence comparison result with the difference of Bacillus genus in the online database is more than 97%, and the JES4 strain and Bacillus aquimaris belong to the same branch. Thus, it was identified as Bacillus aquimaris in combination with the morphology and physiobiochemical characteristics of the colonies and cells.
The strain JES4 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.20301 and the preservation name of the strain Bacillus seawater (Bacillus aquimaris) JES4 in the year 2020, 7 and 06, and the preservation address of the strain is North Chen West Lu No. 1 and 3 in the Korean region of Beijing city.
SEQ ID NO：1
Example 2
Salt tolerance of bacillus seawater (Bacillus aquimaris) JES4
Using inorganic salt medium, adding 0.1% (m/V) yeast powder, setting four different concentration gradients of NaCl treatment: 0. 5.0%, 8.0%, 10.0% and 12.0% (m/V) of JES4 bacteria solution was inoculated to the medium of each treatment group in an amount of 2%, and after inoculation, the culture was carried out in a constant temperature incubator (37 ℃) for 48 hours, and the absorbance of the bacteria solution was measured and repeated three times, and the results are shown in FIG. 1.
As shown in FIG. 3, under the condition of 0-8.0% NaCl salinity, the strain JES4 can grow normally, when the salinity is increased to 10.0%, the strain growth is about one fourth of the maximum growth, the salinity continues to increase, the growth of the strain is obviously inhibited, and the strain cannot grow in the environment with the salt concentration of 12.0%. Therefore, the bacillus seawater (Bacillus aquimaris) JES4 is salt-tolerant, and the salt tolerance degree can reach 12.0% in a liquid culture medium.
Example 3
Growth promotion by bacillus seawater (Bacillus aquimaris) JES4
The JES4 strain fermentation broth which is cultured and reaches the logarithmic growth phase is prepared by the following method: inoculating lawn on slant culture medium into LB liquid culture medium, shake culturing at 37deg.C (120 rpm), transferring to new LB liquid culture medium when it reaches logarithmic phase, and culturing under the same conditions until the concentration is 2.5x10 9 CFU/mL is the required bacterial liquid.
1. Promoting germination.
The wheat variety Jimai No. 22 is used as a material, an experimental group and a control group are arranged, 0.8% NaCl solution is used for soaking absorbent paper, 50 wheat seeds are placed, the experimental group is inoculated with 300 mu L of JES4 bacterial liquid prepared by the method, the control group is not treated, the test group is placed at the optimal germination temperature of 25 ℃ for 5 days, three repetitions are arranged, and the germination rate of the wheat is measured, and the result is shown in figure 4. From FIG. 4, it can be seen that inoculation of JES4 strain at a salt concentration of 0.8% can improve wheat germination.
2. Promoting seedling emergence.
Collecting salinized soil (with salt content of 0.16%), adding NaCl to make salt content of the soil reach 0.8%, setting an experimental group and a control group, adding 2.5mL JES4 strain fermentation broth per 50g of soil after culture to reach logarithmic phase, sieving and mixing uniformly, taking 500g of soil, loading into a culture pot, adding deionized water to adjust water content of the soil to 60%, planting 20 wheat plants of Jimai No. 22 variety, measuring emergence rate after ten days, and repeating each treatment for three times without inoculating JES4 strain, wherein the result is shown in figure 4. As can be seen from fig. 4, after the JES4 strain is inoculated, the wheat emergence rate in the high-salt soil is remarkably improved.
3. Promoting biomass accumulation.
Collecting salinized soil (with salt content of 0.16%), adding NaCl to make salt content of the soil reach 0.5%, setting a test group and a control group, adding cultured JES4 strain fermentation liquor reaching logarithmic phase into the test group, adding 2.5mL JES4 strain fermentation liquor into every 50g of soil, sieving and mixing uniformly, taking 500g of soil, loading into a culture pot, adding deionized water to adjust water content of the soil to 60%, planting "Jimai 22" variety wheat 20, not inoculating JES4 strain into the control group, and weighing underground biomass and overground biomass of wheat after two weeks, and calculating the result shown in figure 5. As can be seen in FIG. 5, after inoculation with JES4 strain, the wheat underground biomass and above-ground biomass increased significantly. It can be seen that the JES4 strain has a remarkable effect of promoting biomass accumulation.
Example 4
Application of bacillus seawater (Bacillus aquimaris) JES4 in actual production
Selecting a salinized land, wherein the salt content of the soil is 0.29%, and setting an experimental group and a control group: the JES4 strain-treated group was an experimental group, and was not inoculated with JES4 strain treatmentIs a control group, each treatment was repeated 3 times with an area of 66.7m 2 . The JES4 strain is treated by seed soaking, culturing to obtain JES4 strain fermentation broth in logarithmic phase (fermentation broth prepared by the method of example 3), and mixing wheat seed (Jimai No. 22) with the bacterial liquid at a ratio of 1:1 (m/V), soaking for 60 min, taking out, draining, and sowing in a test field. The control group is not subjected to bacterial liquid soaking treatment, and other treatments are consistent with the management mode. Wheat yield was measured at harvest and the results are shown in figure 6. From FIG. 6, it is shown that in the salinized soil with a salt content of 0.29%, the yield of wheat seeds treated with the JES4 strain is significantly improved. Therefore, the JES4 strain is adopted for treatment, so that the productivity of the salinized soil can be effectively improved.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to one skilled in the art that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Sequence listing
<110> coastal state college
<120> a strain of bacillus seawater and application thereof in salinized farmland production
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1449
<212> DNA
<213> Bacillus seawater (Bacillus aquimaris)
<400> 1
catcagtccc ttagcggctg gctcaaaagg ttacctcacc gacttcgggt gttacaaact 60
ctcgtggtgt gacgggcggt gtgtacaagg cccgggaacg tattcaccgc ggcatgctga 120
tccgcgatta ctagcgattc cagcttcatg taggcgagtt gcagcctaca atccgaactg 180
agaacggttt tatgggattg gctaaacctc gcggtcttgc tgccctttgt accgtccatt 240
gtagcacgtg tgtagcccag gtcataaggg gcatgatgat ttgacgtcat ccccaccttc 300
ctccggtttg tcaccggcag tcatcttaga gtgcccaact gaatgctggc aactaagatc 360
aagggttgcg ctcgttgcgg gacttaaccc aacatctcac gacacgagct gacgacaacc 420
atgcaccacc tgtcactctg tcccccgaag gggaaagccc tatctctagg gttgtcagag 480
gatgtcaaga cctggtaagg ttcttcgcgt tgcttcgaat taaaccacat gctccaccgc 540
ttgtgcgggc ccccgtcaat tcctttgagt ttcagtcttg cgaccgtact ccccaggcgg 600
agtgcttaat gcgttagctg cagcactaag gggcggaaac cccctaacac ttagcactca 660
tcgtttacgg cgtggactac cagggtatct aatcctgttt gctccccacg ctttcgcgcc 720
tcagtgtcag ttacagacca gaaagtcgcc ttcgccactg gtgttcctcc aaatatctac 780
gcatttcacc gctacacttg gaattccact ttcctcttct gcactcaagt cccccagttt 840
ccaatgaccc tccacggttg agccgtgggc tttcacatca gacttaagga accacctgcg 900
cgcgctttac gcccaataat tccggacaac gcttgccacc tacgtattac cgcggctgct 960
ggcacgtagt tagccgtggc tttctggtta ggtaccgtca aggtaccgcc ctattcgaac 1020
ggtacttgtt cttccctaac aacagagttt tacgatccga aaaccttcat cactcacgcg 1080
gcgttgctcc gtcagacttt cgtccattgc ggaagattcc ctactgctgc ctcccgtagg 1140
agtctgggcc gtgtctcagt cccagtgtgg ccgatcaccc tctcaggtcg gctacgcatc 1200
gtcgccttgg tgagccgtta cctcaccaac tagctaatgc gccgcgggtc catctgtaag 1260
tgatagctaa aagccacctt tcaacatttc ctcatgcgag gaaatgagtt atccggtatt 1320
agccccggtt tcccggagtt atcccagtct tacaggcagg ttacccacgt gttactcacc 1380
cgtccgccgc tgactgatgg gagcaagctc ccatcaatcc gctcgacttg gcatgtatag 1440
cagccgcct 1449
Claims (8)
1. The bacillus seawater is characterized in that the bacillus seawater is bacillus seawater (Bacillus aquimaris) JES4 strain with a preservation number of CGMCC NO.20301.
2. The application of the bacillus marinus with the preservation number of CGMCC NO.20301 in the production of salinized farmlands for improving the germination rate and the emergence rate of wheat, promoting the accumulation of biomass and improving the yield.
3. A microbial preparation for salinized farmland production is characterized by comprising bacillus seawater with a preservation number of CGMCC No.20301.
4. A method for increasing germination rate and emergence rate of crops in salinized farmland, promoting accumulation of biomass and increasing yield is characterized in that fermentation liquor of bacillus subtilis with preservation number of CGMCC No.20301 is used for soaking seeds or fermentation liquor of bacillus subtilis with preservation number of CGMCC No.20301 is inoculated into planting soil, and the crops are wheat.
5. The method for improving germination percentage and emergence rate of crops in salinized farmland, promoting biomass accumulation and improving yield according to claim 4, characterized in that the bacterial concentration of fermentation broth of seawater bacillus with preservation number of CGMCC No.20301 is more than or equal to 2.5x10 9 CFU/mL。
6. The method for improving germination rate and emergence rate of crops in salinized farmland, promoting biomass accumulation and improving yield according to claim 5, characterized in that the fermentation broth of bacillus marinus with preservation number of CGMCC No.20301 is prepared by the following method: inoculating the lawn preserved on the slant culture medium into LB liquid culture medium, shake culturing at 37deg.C, transferring to new LB liquid culture medium when it reaches logarithmic phase, and culturing under the same conditions until it reaches logarithmic phase to obtain the desired fermentation broth.
7. The method for improving germination rate and emergence rate of crops in salinized farmland, promoting biomass accumulation and improving yield according to any one of claims 4-6, wherein the seed soaking is carried out by mixing fermentation liquor of bacillus seawater with preservation number of CGMCC No.20301 with crop seeds to be sown according to volume-mass ratio of 1:1.
8. The method for improving germination rate and emergence rate of crops in salinized farmland, promoting biomass accumulation and improving yield according to any one of claims 4-6, characterized in that the fermentation broth of bacillus marinus with preservation number of CGMCC No.20301 is inoculated into planting soil according to the proportion of inoculating 2.5mL fermentation broth per 50g of soil and uniformly mixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111627771.XA CN114045247B (en) | 2021-12-28 | 2021-12-28 | Bacillus seawater and application thereof in salinized farmland production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111627771.XA CN114045247B (en) | 2021-12-28 | 2021-12-28 | Bacillus seawater and application thereof in salinized farmland production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114045247A CN114045247A (en) | 2022-02-15 |
| CN114045247B true CN114045247B (en) | 2023-04-25 |
Family
ID=80213622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111627771.XA Active CN114045247B (en) | 2021-12-28 | 2021-12-28 | Bacillus seawater and application thereof in salinized farmland production |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114045247B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114437986B (en) * | 2022-02-22 | 2023-06-23 | 南宁师范大学 | Bacillus seawater and screening method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103889235A (en) * | 2010-12-10 | 2014-06-25 | 奥本大学 | Inoculants including bacillus bacteria for inducing production of volatile organic compounds in plants |
| CN105764344A (en) * | 2013-05-31 | 2016-07-13 | 诺维信生物农业公司 | Compositions and methods for enhancing germination |
| CN110462021A (en) * | 2017-01-26 | 2019-11-15 | 拜耳作物科学有限合伙公司 | Promote the method for bacillus spore germination |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107205403B (en) * | 2014-09-30 | 2021-01-08 | 奥本大学 | Use of pectin or pectin-related saccharides to increase the efficacy of plant growth-promoting rhizobacteria (PGPR) strains to promote growth and health in plants and animals |
| CN104974957B (en) * | 2015-06-26 | 2017-10-31 | 滨州学院 | Bacillus（Bacillus sp.）ZY bacterial strains and its application |
| US10524487B2 (en) * | 2017-01-12 | 2020-01-07 | Cisbay | Animal feed stock using microbial enhancement |
| CN108865954B (en) * | 2018-07-30 | 2022-06-10 | 山东福田药业有限公司 | Bacillus marinus and application method thereof in ferment fertilizer |
| CN110846249B (en) * | 2019-11-20 | 2021-07-06 | 山东省土地综合整治服务中心 | Microorganism and microbial agent for improving saline-alkali soil and saline-alkali soil microbial improver |
| CN112940976B (en) * | 2021-03-01 | 2022-05-17 | 河北科技大学 | Marine bacillus megaterium, microbial fertilizer, fermentation broth and application |
-
2021
- 2021-12-28 CN CN202111627771.XA patent/CN114045247B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103889235A (en) * | 2010-12-10 | 2014-06-25 | 奥本大学 | Inoculants including bacillus bacteria for inducing production of volatile organic compounds in plants |
| CN105764344A (en) * | 2013-05-31 | 2016-07-13 | 诺维信生物农业公司 | Compositions and methods for enhancing germination |
| CN110462021A (en) * | 2017-01-26 | 2019-11-15 | 拜耳作物科学有限合伙公司 | Promote the method for bacillus spore germination |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114045247A (en) | 2022-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110577911B (en) | Bacillus pumilus and application thereof | |
| CN109593686B (en) | Shewanella and application thereof in controlling nitrogen pollution of vegetable field soil | |
| CN112940976B (en) | Marine bacillus megaterium, microbial fertilizer, fermentation broth and application | |
| CN111073839B (en) | Siam bacillus, microbial inoculum and application thereof | |
| CN111304126B (en) | Self-generated nitrogen fixing bacterial strain and application thereof | |
| CN113980835A (en) | Compound microbial agent and application thereof in fertilizer | |
| CN114717145B (en) | Cucumber golden fungus and application thereof in degradation of seaweed residues | |
| CN113980834A (en) | Bacillus subtilis and application thereof in fertilizer | |
| CN114045247B (en) | Bacillus seawater and application thereof in salinized farmland production | |
| CN113897320B (en) | Halophyte with salt-tolerant growth-promoting function and application thereof | |
| CN111662846B (en) | Phosphorus-solubilizing bacterium P5, fermentation product, microbial inoculum and application thereof | |
| CN114231425A (en) | Phosphorus-dissolving potassium-solubilizing bacterium Aspergillus niger Z8 and application thereof | |
| CN108570426B (en) | Bacterial strain with phosphate solubilizing function, preparation method of microbial inoculum and microbial inoculum | |
| CN113943684B (en) | Salmonella pinnata and application thereof in salinized farmland production | |
| CN113862195B (en) | Wood fiber degrading composite microbial inoculant containing high-temperature bacteria and fungi and application of wood fiber degrading composite microbial inoculant in tail vegetable compost | |
| CN116426445B (en) | Pseudomonas bacteria NJAU-T102 and application thereof | |
| CN117535210B (en) | Salt-tolerant growth-promoting bacterium, microbial inoculum, preparation method of microbial inoculum and application of microbial inoculum | |
| CN116064288B (en) | Streptomyces roseoformis HC7-22 for plant iron-removing and growth-promoting and application thereof | |
| CN114934000B (en) | Salt-tolerant bacillus amyloliquefaciens and screening and application thereof | |
| CN114409446B (en) | Low-water solid-state fermentation method of drought-tolerant bacteria and application thereof | |
| CN110819561B (en) | Actinomycete TL-007 and application thereof | |
| CN107099468B (en) | Rhodobacter and application thereof | |
| CN107164266B (en) | Micromonospora actinomycetes and application thereof | |
| CN115956502A (en) | Application of streptomyces roseoflavus LN33 in aspect of promoting growth and development of tobacco | |
| CN115011521A (en) | Enterobacter cloacae YES1 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |