CN103467144B - Refined fulvic-acid-containing agricultural microbial inoculum and production method thereof - Google Patents
Refined fulvic-acid-containing agricultural microbial inoculum and production method thereof Download PDFInfo
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Abstract
The invention discloses a refined fulvic-acid-containing microbial inoculum and a production method thereof, and relates to the microbial inoculum using biological humic acid and functional bacteria as main raw materials and the production method thereof. The refined fulvic-acid-containing microbial inoculum is prepared by mixing 75 to 80 parts by weight of biological humic acid powder, 10 to 15 parts by weight of an active humic acid liquid, 1.5 parts by weight of bacillus subtilis microbial inoculum and 8.5 parts by weight of a binder, and is a fine grained product with the moisture content of 12%-15%. In the product, organic matters account for 56-58% of the total dry matter weight of the product, fulvic acid in the organic matters accounts for 15.5-16.2% of the total dry matter weight of the product, the living bacterium quantity of bacillus subtilis is more than or equal to 2*108/g, and the fine grained shape means that the weight of fine grains with the size of 0.5-2 mm in the product accounts for more than 80% of the total weight of the product. In the product, the fulvic acid content is high, the functional bacteria content is rich, the fine grained agent form is good, the use amount is low, and the product is suitable for various application modes, all crops and cultivated lands, and has very significant effects on improvement of soil and promotion of production increasing.
Description
Technical field
The present invention relates to a kind of is main raw material containing humic acid agricultural microbiobacterial agent and manufacture method thereof with biological humic and function yeast.
Background technology
Biological humic is collective's innovation achievement of a large amount of scientific worker of China beginning of this century last century Mo, utilize with microbial technique the organic active substance transforming organic waste, its most important effective ingredient is xanthohumic acid (FA), most solid (powdery) biological humic also contains function yeast (B), so this kind of humic acids is abbreviated as BFA again.This professional term of biological humic and product class have announced in China Humic Acid TIA in 2007 annual meeting (Beijing) to be determined, and has done reflection in the academic documents such as " humic acids application book series " published and distributed in chemical industry press.
Recent two decades comes, biological humic product mainly circulates in agricultural materials market with " starter " of powdery and aqueous " fertilizer for spraying on leaf surface " two kinds of forms, powder, liquid divide two lines, every bar line application surface is all narrow, constrain the large-scale promotion application of this series products, be also difficult to make agriculture field obtain support and the transformation of biological humic technical spirit.
Our company obtained two patents of invention (ZL03138767.5 and ZL03112360.0) once, for many years production and selling biological humic pulvis and activated humic acid liquid, and these two kinds of products have unusual effect to improvement soil and raising crop yield.But biological humic powder, except except doing and having good market response in fertilizer starter, because it is in very light finely powdered, uses inconvenience, be directly used in that farmland is more difficult pushes open.Activated humic acid liquid sprays for the manufacture of fertilizer for spraying on leaf surface, and each consumption is restricted, limited to the effect of increasing production of crop, and the more difficult expansion in water-deficient area, so consumption is hovered at low level always.
The head Two decades years (1990-2010) that biological humic technology is firstly appeared, it is theoretical that insider continues to use mineral humic acids, thus only applying biological humic acids product in fertilizer for spraying on leaf surface and common fertilizer manufacture two, although demonstrated biological humic product to have superiority than mineral humic acids, but can not be formed agriculturally to the large-scale application of biological humic product all the time, and biological humic insider is in the face of the organic waste of annual billions of ton (dry-matter), also just can only be on small scale, can not wonderful works be had.Because the market consumption of the main product for agriculture of above-mentioned two class limits the large-scale application to biological humic product.
Summary of the invention
The present invention aims to provide a kind of refining containing xanthohumic acid agricultural microbial agent, and its consumption is few, effect strong, is applicable to various farmlands and uses; And the manufacture method of this agricultural microbial agent is provided.
Technical scheme of the present invention is: a kind of refining containing xanthohumic acid agricultural microbial agent, it is the fine-grannular and the product of water ratio 12%-15% that are mixed by the cakingagent of the activated humic acid liquid of the biological humic powder of 75-80 weight part, 10-15 weight part, the bacillus subtilis microbial agent of 1.5 weight parts and 8.5 weight parts, in this product effective ingredient have weight account for this product dry-matter gross weight 56-58% organic matter and in this organic matter the weight of xanthohumic acid account for viable bacteria amount>=2 × 10 of this product dry-matter gross weight 15.5%-16.2%, subtilis
8individual/gram; So-called fine-grannular product refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter; The fineness of biological humic powder wherein at 60-80 order and water ratio 9%-10%, have in this biological humic flakes dry matter weight account for its gross weight 65%-67% organic matter and in this organic matter the weight of xanthohumic acid account for the 15.5%-17% of this biological humic flakes dry matter gross weight; Activated humic acid liquid water ratio 45%-50% wherein, have in this activated humic acid liquid weight account for its gross weight 25%-27% organic matter and in this organic matter the weight of xanthohumic acid account for the 18%-20% of this activated humic acid liquid gross weight; Cakingagent is wherein that fineness is at 60-80 object granulation of fertilizer clay; Viable bacteria amount>=2 × 10 of bacillus subtilis microbial agent wherein
10individual/gram.
The above-mentioned refining manufacture method containing xanthohumic acid agricultural microbial agent, the step comprised has:
Step one, pre-mixing; The cakingagent of the biological humic powder of 75-80 weight part, the activated humic acid liquid of 10-15 weight part and 8.5 weight parts is added in two ribbon mixer and mix 5-10 minute, make Preblend; The fineness of biological humic powder wherein at 60-80 order and water ratio 9%-10%, have in this biological humic flakes dry matter weight account for its gross weight 65%-67% organic matter and in this organic matter the weight of xanthohumic acid account for the 15.5%-17% of this biological humic flakes dry matter gross weight; Activated humic acid liquid water ratio 45%-50% wherein, have in this activated humic acid liquid weight account for its gross weight 25%-27% organic matter and in this organic matter the weight of xanthohumic acid account for the 18%-20% of this activated humic acid liquid gross weight; Wherein cakingagent is that fineness is at 60-80 object granulation of fertilizer clay;
Step 2, die mould and fragmentation; Above-mentioned Preblend is added in rolling-type tablets press or rotating-expressing type squeezing machine and be pressed into particle or flap, above-mentioned particle or flap are added and be not cut into fine-grannular woollen with in the claw crusher of screen cloth, in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter;
Step 3, secondary mixes; By viable bacteria amount>=2 × 10 of above-mentioned fine-grannular woollen and 1.5 weight parts
10individual/gram bacillus subtilis microbial agent put into tipping bucket type mixing machine mixing 5-10 minute, make fine-grannular and the product of water ratio 12%-15%, in this product effective ingredient have weight account for this product dry-matter gross weight 56-58% organic matter and in this organic matter the weight of xanthohumic acid account for the 15.5%-16.2% of this product dry-matter gross weight, viable bacteria amount>=2 × 10 of subtilis
8individual/gram.
Biological humic powder (BFA) mixes with activated humic acid liquid and appropriate tamanori (clay) (mixing machine can adopt two ribbon mixer) by method of the present invention; by roll extrusion granulator granulation; or make sheet through rotating-expressing type squeezing machine; again through the claw crusher in crushing beading shape not with screen cloth; then mix with bacillus subtilis microbial agent at tipping bucket type mixer, just can pack sale.The course of processing is dry-type processing, exempts from drying or airing, remains the physiologically active of humic acids, makes the function yeast in starting material be able to whole reservation.
Method of the present invention is by biological humic powder, activated humic acid liquid " two bar line " into a line, and the bacterium bacillus subtilis microbial agent simultaneously also adding high bacterium amount produces containing xanthohumic acid agricultural microbial agent.This invention will make product reach the product characteristics of " fine-grannular ", make biological humic powder and these two kinds of major ingredients of activated humic acid liquid fully fused under the help of clay, simultaneously again can be easy to use, convenient in soil rapid permeability.But also do not have suitable machinery to can be used to directly manufacture the fines (dry type granulation) of 0.5-2 millimeter at present, therefore the first dry type roll extrusion of method choice of the present invention or spinning, output macrobead or blade, be then broken into the fines of 0.5-2 millimeter.
Design of the present invention derives from contriver in recent years to the theory innovation of biological humic subject.Over the past two years, the lot of experiments of contriver and put into practice shocking, reasonable disposition biological humic powder, activated humic acid liquid, and combine with function yeast subtilis, farm crop application produces incredible effect: every mu every batch crop uses 7.5-10KG, larger than the control group volume increase 15-50%(inhomogeneity crop comparison in difference of same water and fertilizer management), there is the situation of soil-borne disease or natural disaster, stimulation ratio is higher, and crops quality is better.Utilize the said products and chemical fertilizer used in combination, or to spread manuer in holes separately, or mixed water-soluble watering is executed, and can produce the effect using bio-organic fertilizer, but its consumption of equivalent efficacy is only about 10% of general biological organic fertilizer.Emergency crops every batch is only used once, and long-term crop is used 2-3 time every year, just can play improvement soil, improve the effect of chemical fertilizer utilization ratio, increase yield and lifting crop quality.Through many places, application result proves, uses 10KG for every mu at every turn, and various crop yield is as follows: block root tuber crops volume increase 30-40%; Melon eggplant green pepper beans volume increase 25-30%; Leaf vegetables volume increase 20-25%; Fruits volume increase 10-15%; Standing grain section grain class stimulation ratio is higher; In the land use of continuous cropping, soil-borne disease, or there is drought, flood, the disaster such as to freeze, increase production more than 50% on year-on-year basis.Adhere to using product of the present invention, soil can continue farming forever, and this is significant to agricultural modernization.Apply a large amount of microbial inoculum with independent again or apply separately compared with humic acids, the result of use of said mixture is also obviously much better.This makes us recognize: continue to use mineral humic acids theory (absorption, chelating, complexing, cation replacement etc.), cannot explain above biological humic application phenomenon, and biological humic subject must found oneself theory and technology system.
Below the theoretical frame of our Primary Construction:
Which type of fertilizer should one, we manufacture?
The fertilizer manufacturing process of traditional classical is aerobic fermentation repeatedly turning, hyperthermia drying, produce " mineralising soil ulmin ", this technique is converted into Carbon emission in a large number the carbon in material and falls, the microorganism that fermenting process produces also exhausts, fertilizer loses its core value---activated humic acid and microorganism, and this fertilizer is soil improvement agent at most.
Does two, why soil harden, and is that " using chemical fertilizer in a large number " causes?
We find wetland or the river bank at some edges, farmland, and the content of chemical fertilizer is also higher than neighbouring soil content, but has many earthworms, this illustrate earthworm " do not poisoned with poison " by chemical fertilizer, but died of hunger or starve run.Because soil organic matter content is too low, earthworm has not had food.In like manner, very little, make carbon-nitrogen ratio too low, microorganism is irreproducible, therefore soil compaction for soil with organic matter.Visible toward soil add water-soluble organic matter (being rich in activated humic acid) just can make soil reappear activity, the main points of the biological humic acid fertilizer mechanism of action that Here it is it
Three, use the ferment-fermented fertilizer of biological humic class can not turning why, thus produce the Organic fertilizer being rich in activated humic acid and microorganism?
Because biological humic production process is natural comprise multiclass bacterium, aerobic, facultative and anerobe, and humic acids provides the initial energy to microorganism, the metabolism of microorganism produces xanthohumic acid again, both combine constantly can promote cyclic extensions mutually under certain condition, and this is the relation of " egg white " and " yolk ", is 1+1 > 2, it is possible that this has made not turning be fermented into, and is also the reason making soil quick improvement.
Four, the production-increasing function of product of the present invention can not be explained by the theory of " chemical fertilizer fertilising ".
From mathematical logic Epidemiological Analysis, the growth effect that a kind of factor causes is the relation of linear growth, the growth effect that two kinds of factors cause is exactly the relation with increase of binary or quadratic power, and the growth effect that the multidimensional and multiple-level face factor " general mobilization " is caused will produce the phenomenon of similar geometricprogression.Therefore the Increase production ability of product of the present invention can not be explained by the theory of " chemical fertilizer fertilising ".The use of product of the present invention can improve soil three kinds of fertility simultaneously: physics fertility, Chemical fertility (promoting carbon " short slab ") and biological fertility, the lifting of these three kinds of fertility interacts again and promotes mutually, thus causes the especially big lifting to crop yield quality.Metaphor by a kind of image: " trigger electrode " (or controlling pole) of the similar triode of product of the present invention, just can get through macro-energy passage to a little energy.
Does is five, what the basis of biological humic theory?
China's fertilizer, biological organic fertilizer industry are passed by very long winding raod, and production technique current so far still exists major defect, and its reason is the disappearance of new theory or the misleading of old theory mainly.Fertilizer scientific worker should catch these two fertile core---water-soluble organic matter (activated humic acid) and functional microorganisms of planting, carry out technology and process innovation, the development of this two classes fertilizer could be drawn upper correct road, this also should be the basis of biological humic theory.
What the present invention refined contains xanthohumic acid agricultural microbial agent, biological humic powder, activated humic acid liquid are added pure function bacterium bacillus subtilis microbial agent three to be blended together use, confirm through a large amount of field test: this is a kind of practicality, have biological organic fertilizer function and consumption is only " containing the xanthohumic acid agricultural microbial agent " of ordinary student organic fertilizer about 10%.This fertilizer xanthohumic acid content is high, functional bacterial content abundant, physical behavior is fine-grannular, and formulation is good, consumption is few, is adapted to various method of application: can be mixed with chemical fertilizer, also can use separately; Can directly spread fertilizer over the fields, also miscible watering in water is executed, is rushed and execute.Being applicable to all crops and all places, is a kind of concentrated type biological organic fertilizer kind conveniently using popularization.Such as:
One, paddy rice application test
Time: 2011.7.20-2011.11.15;
Experiment material: test group enriches refining containing xanthohumic acid agricultural microbial agent every mu of 10KG, other water and fertilizer managements and control group are together.
Experimental result is as following table
Discussion: from test-results, what interpolation was refining contains xanthohumic acid agricultural microbial agent group, and paddy thousand seed weight increases, and commodity paddy stimulation ratio is higher than gross weight stimulation ratio, illustrates that in paddy, substandard products are few, and this all reflects paddy not only output increase, and Quality advance.
Two, balsam pear application test
Time: 2011.9.5-2012.1.10
Experiment material: test group enriches refining containing xanthohumic acid agricultural microbial agent every mu of 10KG, other water and fertilizer managements and control group are together.
Experimental result is as following table
Discuss: from test-results, add and refining extend 16 days containing the xanthohumic acid agricultural microbial agent group collecting period, and production unit cost is higher than control group, it is higher than stimulation ratio to be reflected in the rate of increasing income.
Three, leafiness Chinese cabbage application practice
Time: 2011.12.26-2012.2.2
Experiment material: the pure subtilis (2 × 10 of A group
10individual/g) every mu of 8.3KG; What B group was refining contains xanthohumic acid agricultural microbial agent every mu of 8.3KG; C group biological humic powder every mu of 8.3KG; CK group is above every for not adding, and other water and fertilizer managements are respectively organized identical.
Experimental result is as following table
| Group | Per mu yield (KG) | Increase production (KG) than mu with CK | Stimulation ratio is compared with CK |
| A | 3618.9 | 223.8 | 6.6% |
| B | 4175.0 | 779.9 | 23.0% |
| C | 3757.5 | 362.4 | 10.6% |
| CK | 3395.1 | ---- | ---- |
Result proves: three kinds of material (pure function bacterium 2 × 10
10individual/g, refining containing xanthohumic acid agricultural microbial agent and biological humic powder) there is effect of increasing production, be arranged as with effect size (effect):
Refining contains humic acid agricultural microbiobacterial agent > biological humic powder > pure function bacterium 2 × 10
10individual/g.
Four, Caulis et Folium Brassicae capitatae application experiment
Time: 2011.10.12-2012.03.05
Experiment material: A group every mu is affixed by 100KG biological organic fertilizer; B group every mu is affixed by 150KG biological organic fertilizer; C group every mu is affixed by refining containing xanthohumic acid agricultural microbial agent 10KG; CK group is not for add.Water and fertilizer management is respectively organized identical, and every mu uses 16-16-16 unexpected rival's board composite fertilizer 100KG to make base manure.
Experimental result is as following table
Discuss: from upper table result: on the basis that every mu is used 100KG composite fertilizer, add fertilizer and refining all increasing production to some extent than comparative group containing xanthohumic acid agricultural microbial agent, and it be as follows to increase production size order:
B(150KG biological organic fertilizer) > C(10KG refining containing xanthohumic acid agricultural microbial agent) > A(100KG biological organic fertilizer)
Tentatively can judge thus: the refining biological organic fertilizer being slightly less than its 15 times amount containing xanthohumic acid agricultural microbial agent effect of increasing production, and be greater than the biological organic fertilizer of its 10 times amount.
Five, potato application test
Time: on March 15,13 days to 2012 November in 2011
Experiment material: experimental group add every mu of 10KG refining containing xanthohumic acid agricultural microbial agent, control group does not add.Base manure equally every mu execute 100KG16-16-16 unexpected rival's board composite fertilizer, control measures are identical.
Application experiment result is as following table
Discuss: from upper table result, test group (every mu adds refining containing xanthohumic acid agricultural microbial agent 10KG), per mu yield and the output value all have than control group obviously to be increased, from seedling rate and soil moisture content, add refining containing after xanthohumic acid agricultural microbial agent, soil is improved very well, and this causes, and high, the qualified fruit rate of potato seedling rate is high, fruit even, so the mu rate of increasing output value is higher than per mu yield rate of increase.From the test of this group, refining may be remarkable especially to the effect of increasing production of stem tuber block root crop containing xanthohumic acid agricultural microbial agent.
Experiment prove product of the present invention there is very strong effect of increasing production: equivalent efficacy consumption need only ordinary student organic fertilizer about 10%, emergency crops uses 1 time, every mu of 7.5-10KG, can increase production more than 20%; Normal annual crop every mu is executed 2-3 time every year, and 15-20KG, can increase production more than 15% altogether.In other words, each amount of application of land area of one unit is also fewer than chemical fertilizer, solves fertilizer and bio-organic fertilizer consumption large, and labor cost high and booth, mountain region etc. are difficult to a difficult problem for manure use and biological organic fertilizer.Therefore, product of the present invention is on the progress of agricultural and fertilizer circle with change and will produce important and far-reaching impact.And produce biological humic powder and the activated humic acid liquid raw material of this product, all derive from the recycling to organic waste.So product of the present invention is applied in the large-scale popularization of agriculture field, will greatly promote national environmental protection cause and carbon emission reduction, catch up with and surpass developed country for China's agricultural civilization and make contributions.
Accompanying drawing explanation
Fig. 1 be the present invention refine containing xanthohumic acid agricultural microbial agent product characteristics photo.
Fig. 2 is the Production Flow Chart schematic diagram of the manufacture method containing xanthohumic acid agricultural microbial agent that the present invention refines.
Embodiment
One, embodiment one
What manufacture the present invention refined contains xanthohumic acid agricultural microbial agent, its specification of quality is: product is fine-grannular and water ratio 12%-15%, in this product effective ingredient have weight account for this product dry-matter gross weight 56-58% organic matter and in this organic matter the weight of xanthohumic acid account for viable bacteria amount>=2 × 10 of this product dry-matter gross weight 15.5%-16.2%, subtilis
8individual/gram; So-called fine-grannular refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter, please refer to Fig. 1.
Manufacture the process containing xanthohumic acid agricultural microbial agent that the present invention refines, please refer to Fig. 2.
Step one, pre-mixing; The cakingagent of the biological humic powder of 75KG, the activated humic acid liquid of 15KG and 8.5KG is added in two ribbon mixer and mix 5-10 minute, make Preblend.Biological humic powder in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03138767.5 patent.The water ratio of this biological humic powder is 10%, weight organic in the dry-matter of this biological humic powder be this biological humic flakes dry matter weight 65% and in this organic matter the weight of xanthohumic acid be 15.5% of this biological humic flakes dry matter weight, the fineness of this biological humic powder is at 60-80 order.Activated humic acid liquid in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03112360.0 patent.This activated humic acid liquid water ratio 45%-50%, has weight to account for its gross weight 25% organic matter in this activated humic acid liquid and in this organic matter, the weight of xanthohumic acid accounts for 18% of this activated humic acid liquid gross weight; Cakingagent is wherein that fineness is at 60-80 object granulation of fertilizer clay.
Step 2, die mould and fragmentation; Above-mentioned Preblend is added in rolling-type tablets press and is pressed into particle; or rotating-expressing type squeezing machine is extruded into sheet; above-mentioned particle or flap are added and be not cut into fine-grannular woollen with in the claw crusher of screen cloth, in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter.
Step 3, secondary mixes; By viable bacteria amount>=2 × 10 of above-mentioned fine-grannular woollen and 1.5KG
10individual/gram bacillus subtilis microbial agent put into tipping bucket type mixing machine mixing 5-10 minute, make granuliform finished product.Bacillus subtilis microbial agent wherein can be bought to producers such as Beihai Qun Lin biotechnology company limiteds.
The refining effective ingredient containing xanthohumic acid agricultural microbial agent of preparation like this is calculated as follows:
1, dry-matter:
75KG×90%+15KG×50%+8.5KG+1.5KG
=67.5KG+7.5KG+8.5KG+1.5KG=85.0KG
2, water ratio:
（75KG×10%+15KG×50%）/（75KG+15KG+8.5KG+1.5KG）
=（7.5KG+7.5KG）/100KG=15%
3, organic:
The contribution of biological humic powder is organic: 75KG × 90% × 65%=43.87KG
The contribution of activated humic acid liquid is organic: 15KG × 25%=3.75KG
Add up to organic: 43.87KG+3.75KG=47.62KG
Organic content in the dry-matter of product: 47.62KG/85.0KG=56%
4, xanthohumic acid:
Biological humic powder contribution xanthohumic acid: 75KG × 90% × 15.5%=10.46KG
Activated humic acid liquid contribution xanthohumic acid: 15KG × 18%=2.7KG
Add up to xanthohumic acid total amount: 10.46KG+2.7KG=13.16KG
Xanthohumic acid content: 13.16KG/85.0KG=15.5% in product dry-matter
5, bacillus subtilis bacterial content in product:
（1.5KG×2×10
10/g）/（75KG+15KG+8.5KG+1.5KG）
=3×10
8/g
Consider product preservation period rate of fall-off, by 2 × 10
8/ g evaluates, and meets the standard of GB20287-2006 " living bacteria count ".
From calculating above: the quality index meeting design code by the product of this formulated.
Two, embodiment two
What manufacture the present invention refined contains xanthohumic acid agricultural microbial agent, its specification of quality is: product is fine-grannular and water ratio 12%-15%, in this product effective ingredient have weight account for this product dry-matter gross weight 56-58% organic matter and in this organic matter the weight of xanthohumic acid account for viable bacteria amount>=2 × 10 of this product dry-matter gross weight 15.5%-16.2%, subtilis
8individual/gram; So-called fine-grannular refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter, please refer to Fig. 1.
Manufacture the process containing xanthohumic acid agricultural microbial agent that the present invention refines, please refer to Fig. 2:
Step one, pre-mixing; The cakingagent of the biological humic powder of 80KG, the activated humic acid liquid of 10KG and 8.5KG is added in two ribbon mixer and mix 5-10 minute, make Preblend.Biological humic powder in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03138767.5 patent.The water ratio of this biological humic powder is 9%, weight organic in this biological humic flakes dry matter account for this biological humic flakes dry matter weight 67%, the weight of xanthohumic acid accounts for 17% of this biological humic flakes dry matter weight in this organic matter, the fineness of this biological humic powder is at 60-80 order.Activated humic acid liquid in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03112360.0 patent.This activated humic acid liquid water ratio is 45%, weight organic in this activated humic acid liquid account for its gross weight 27%, the weight of xanthohumic acid accounts for 20% of its gross weight in this activated humic acid liquid; Cakingagent is wherein that fineness is at 60-80 object granulation of fertilizer clay.
Step 2, die mould and fragmentation; Above-mentioned Preblend is added rolling-type tablets press to make in particle or rotating-expressing type squeezing machine and be pressed into flap; above-mentioned particle or flap are added and be not cut into fine-grannular woollen with in the claw crusher of screen cloth, in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter.
Step 3, secondary mixes; By viable bacteria amount>=2 × 10 of above-mentioned fine-grannular woollen and 1.5KG
10individual/gram bacillus subtilis microbial agent put into tipping bucket type mixing machine mixing 5-10 minute, make granuliform finished product.Bacillus subtilis microbial agent in its raw material can be bought to producers such as Beihai Qun Lin biotechnology company limiteds.
The refining effective ingredient containing xanthohumic acid agricultural microbial agent of preparation like this is calculated as follows:
1, dry-matter:
80KG×（100-9）%+10KG×（100-45）%+8.5KG+1.5KG
=72.8KG+5.5KG+8.5KG+1.5KG=88.3KG
2, water ratio:
（80KG×9%+10KG×45%）/（75KG+15KG+8.5KG+1.5KG）
=（7.2KG+4.5KG）/100KG=11.7%≈12%
3, organic:
The contribution of biological humic powder is organic: 80KG × (100-9) × 67%=48.78KG
The contribution of activated humic acid liquid is organic: 10KG × 27%=2.7KG
Add up to organic: 48.78KG+2.7KG=51.58KG
Organic content in product dry-matter: 51.58KG/88.3KG=58.44% ≈ 58%
4, xanthohumic acid:
Biological humic powder contribution xanthohumic acid: 80KG × (100-9) × 17%=12.34KG
Activated humic acid liquid contribution xanthohumic acid: 10KG × 20%=2.0KG
Add up to xanthohumic acid total amount: 12.34KG+2.0KG=14.34KG
Xanthohumic acid content: 14.34KG/88.3KG=16.2% in product dry-matter
5, bacillus subtilis bacterial content in product:
（1.5KG×2×10
10/g）/（80KG+10KG+8.5KG+1.5KG）
=3×10
8/g
Consider product preservation period rate of fall-off, by 2 × 10
8/ g evaluates, and meets the standard of GB20287-2006 " living bacteria count ".
From calculating above: the quality index meeting design code by the product of this formulated.
Three, embodiment three
What manufacture the present invention refined contains xanthohumic acid agricultural microbial agent, its specification of quality is: product is fine-grannular and water ratio 12%-15%, in this product effective ingredient have weight account for this product dry-matter gross weight 56-58% organic matter and in this organic matter the weight of xanthohumic acid account for viable bacteria amount>=2 × 10 of this product dry-matter gross weight 15.5%-16.2%, subtilis
8individual/gram; So-called fine-grannular refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter, please refer to Fig. 1.
Manufacture the process of refined biological organic fertilizer material of the present invention, please refer to Fig. 2:
Step one, pre-mixing; The cakingagent of the biological humic powder of 77.5KG, the activated humic acid liquid of 12.5KG and 8.5KG is added in two ribbon mixer and mix 5-10 minute, make Preblend.Biological humic powder in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03138767.5 patent.The water ratio 9.5% of this biological humic powder, weight organic in this biological humic flakes dry matter account for this biological humic flakes dry matter weight 66%, the weight of xanthohumic acid accounts for 16% of this biological humic flakes dry matter weight in this organic matter, the fineness of this biological humic powder is at 60-80 order.Activated humic acid liquid in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03112360.0 patent.This activated humic acid liquid water ratio 48%, weight organic in this activated humic acid liquid account for its gross weight 26%, the weight of xanthohumic acid accounts for 19.2% of its gross weight in this activated humic acid liquid; Cakingagent is wherein that fineness is at 60-80 object granulation of fertilizer clay.
Step 2, die mould and fragmentation; Above-mentioned Preblend is added in rolling-type tablets press or rotating-expressing type squeezing machine and be pressed into particle or flap; above-mentioned particle or flap are added and be not cut into fine-grannular woollen with in the claw crusher of screen cloth, in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter.
Step 3, secondary mixes; By viable bacteria amount>=2 × 10 of above-mentioned fine-grannular woollen and 1.5KG
10individual/gram bacillus subtilis microbial agent put into tipping bucket type mixing machine mixing 5-10 minute, make granuliform finished product.Bacillus subtilis microbial agent wherein can be bought to producers such as Beihai Qun Lin biotechnology company limiteds.
The refining effective ingredient containing xanthohumic acid agricultural microbial agent of preparation like this is calculated as follows:
1, dry-matter:
77.5KG×（100-9.5）%+12.5KG×（100-48%）+8.5KG+1.5KG
=70.14KG+6.5KG+8.5KG+1.5KG=86.64KG
2, water ratio:
（77.5KG×9.5%+12.5KG×48%）/（75KG+15KG+8.5KG+1.5KG）
=（7.36KG+6.0KG）/100KG=13.4%≈13%
3, organic:
The contribution of biological humic powder is organic: 77.5KG × (100-9.5) × 66%=46.21KG
The contribution of activated humic acid liquid is organic: 12.5KG × 19.2%=2.4KG
Add up to organic: 46.21KG+2.4KG=48.61KG
Organic content in product dry-matter: 48.61KG/86.64KG=57.08% ≈ 57%
4, xanthohumic acid:
Biological humic powder contribution xanthohumic acid: 77.5KG × (100-9.5) × 16%=11.22KG
Activated humic acid liquid contribution xanthohumic acid: 12.5KG × 19.2%=2.4KG
Add up to xanthohumic acid acid total amount: 11.22KG+2.4KG=13.62KG
Xanthohumic acid content in product dry-matter: 13.62KG/86.64KG=15.7% ≈ 16%
5, bacillus subtilis bacterial content in product:
（1.5KG×2×10
10/g）/（77.55KG+12.5KG+8.5KG+1.5KG）
=3×10
8/g
Consider product preservation period rate of fall-off, by 2 × 10
8/ g evaluates, and meets the standard of GB20287-2006 " living bacteria count ".
From calculating above: the quality index meeting design code by the product of this formulated.
The above, be only present pre-ferred embodiments, do not limit scope of the invention process with this, and equivalence change and the modification done according to technical scheme of the present invention and description, all should belong to the scope that the present invention is contained.
Claims (1)
1. a refining manufacture method containing xanthohumic acid agricultural microbial agent, what this was refining is by the biological humic powder of 75-80 weight part containing xanthohumic acid agricultural microbial agent, the activated humic acid liquid of 10-15 weight part, the fine-grannular that the bacillus subtilis microbial agent of 1.5 weight parts and the cakingagent of 8.5 weight parts are mixed and the product of water ratio 12%-15%, in this product effective ingredient have weight account for this product dry-matter gross weight 56-58% organic matter and in this organic matter the weight of xanthohumic acid account for this product dry-matter gross weight 15.5%-16.2%, viable bacteria amount>=2 × 10 of subtilis
8individual/gram, so-called fine-grannular product refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter, the fineness of biological humic powder wherein at 60-80 order and water ratio 9%-10%, have in this biological humic flakes dry matter weight account for its gross weight 65%-67% organic matter and in this organic matter the weight of xanthohumic acid account for the 15.5%-17% of this biological humic flakes dry matter gross weight, activated humic acid liquid water ratio 45%-50% wherein, have in this activated humic acid liquid weight account for its gross weight 25%-27% organic matter and in this organic matter the weight of xanthohumic acid account for the 18%-20% of this activated humic acid liquid gross weight, cakingagent is wherein that fineness is at 60-80 object granulation of fertilizer clay, viable bacteria amount>=2 × 10 of bacillus subtilis microbial agent wherein
10individual/gram, manufacture above-mentioned refining containing xanthohumic acid agricultural microbial agent, the step comprised has:
Step one, pre-mixing; The cakingagent of the biological humic powder of 75-80 weight part, the activated humic acid liquid of 10-15 weight part and 8.5 weight parts is added in two ribbon mixer and mix 5-10 minute, make Preblend; The fineness of biological humic powder wherein at 60-80 order and water ratio 9%-10%, have in this biological humic flakes dry matter weight account for its gross weight 65%-67% organic matter and in this organic matter the weight of xanthohumic acid account for the 15.5%-17% of this biological humic flakes dry matter gross weight; Activated humic acid liquid water ratio 45%-50% wherein, have in this activated humic acid liquid weight account for its gross weight 25%-27% organic matter and in this organic matter the weight of xanthohumic acid account for the 18%-20% of this activated humic acid liquid gross weight; Wherein cakingagent is that fineness is at 60-80 object granulation of fertilizer clay;
Step 2, die mould and fragmentation; Above-mentioned Preblend is added in rolling-type tablets press or rotating-expressing type squeezing machine and be pressed into particle or flap, above-mentioned particle or flap are added and be not cut into fine-grannular woollen with in the claw crusher of screen cloth, in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter;
Step 3, secondary mixes; By viable bacteria amount>=2 × 10 of above-mentioned fine-grannular woollen and 1.5 weight parts
10individual/gram bacillus subtilis microbial agent put into tipping bucket type mixing machine mixing 5-10 minute, make fine-grannular and the product of water ratio 12%-15%, in this product effective ingredient have weight account for this product dry-matter gross weight 56-58% organic matter and in this organic matter the weight of xanthohumic acid account for the 15.5%-16.2% of this product dry-matter gross weight, viable bacteria amount>=2 × 10 of subtilis
8individual/gram.
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| CN109762568A (en) * | 2019-03-08 | 2019-05-17 | 河南沐天农业科技有限公司 | A kind of preparation method and applications of fulvic acid biological bacteria modifying agent |
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|---|---|---|---|---|
| CN1199722A (en) * | 1997-05-21 | 1998-11-25 | 王家祥 | Bacterial active humate and its production |
| CN1974490A (en) * | 2006-12-11 | 2007-06-06 | 陈五岭 | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure |
| CN101602948A (en) * | 2008-06-10 | 2009-12-16 | 丁之铨 | A kind of multifunctional soil microorganism restorative |
| CN102174583A (en) * | 2011-02-22 | 2011-09-07 | 福州大学 | Method for producing biological humic acid by mixed fermentation |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1199722A (en) * | 1997-05-21 | 1998-11-25 | 王家祥 | Bacterial active humate and its production |
| CN1974490A (en) * | 2006-12-11 | 2007-06-06 | 陈五岭 | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure |
| CN101602948A (en) * | 2008-06-10 | 2009-12-16 | 丁之铨 | A kind of multifunctional soil microorganism restorative |
| CN102174583A (en) * | 2011-02-22 | 2011-09-07 | 福州大学 | Method for producing biological humic acid by mixed fermentation |
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Address after: 363000 Fujian Zhangzhou Zhaoan County four Zhang Gong Private Economic Zone Patentee after: FUZHOU LYUZHOU BIOCHEMICAL CO., LTD. Patentee after: Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agriculture Sciences Address before: 363000 Fujian Zhangzhou Zhaoan County four Zhang Gong Private Economic Zone Patentee before: Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov. Patentee before: Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agriculture Sciences |