CN109762568A - A kind of preparation method and applications of fulvic acid biological bacteria modifying agent - Google Patents
A kind of preparation method and applications of fulvic acid biological bacteria modifying agent Download PDFInfo
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- CN109762568A CN109762568A CN201910173762.4A CN201910173762A CN109762568A CN 109762568 A CN109762568 A CN 109762568A CN 201910173762 A CN201910173762 A CN 201910173762A CN 109762568 A CN109762568 A CN 109762568A
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Abstract
The present invention provides a kind of preparation method and applications of fulvic acid biological bacteria modifying agent, its main component fulvic acid can not only selective absorption toxic heavy metal, to in soil must element complex reaction can occur, increase the biological effectiveness of essential elements, and soil fertility can be increased, its main component bacillus subtilis and bacillus megaterium can be good at adaptability, it cooperates, it can be good at solving Pesticide Residue in Soil organic substance residues, remaining chemical fertilizer in degradation soil, improve Soil Respiration, improve soil physico-chemical property, the problem of comprehensive treatment soil pollution, secondary phytotoxicity will not be generated.
Description
Technical field
The invention belongs to soil improvement fields, and in particular to a kind of preparation method of fulvic acid biological bacteria modifying agent and its answer
With.
Background technique
Soil pollution refers to the development of economy and society, generating various pollutants in human industry or agricultural production process
Enter soil environment accumulation by different approaches, eventually exceeds soil self purification activity, soil quality is made to change with function, endanger
And the mankind and other biological the survival and development the phenomenon that, it is aobvious according to national Soil Pollution Investigation bulletin result in 2014
Show, the total exceeding standard rate of national soil is 16.1%, and overall state allows of no optimist, wherein exceeded digit of agricultural ground contamination accounts for about agriculture
The 19% of land used total number of sample points is the major fields of China's current soil prevention and cure of pollution；China's soil pollution overall situation is non-
Chang Yanjun is mainly manifested in the sides such as fertilizer element accumulation, various heavy is seriously polluted, pesticide and organic pollutant residual quantity height
Face, thereafter Tab phenolphthaleinum cause soil hardening, salinization of soil, nutrient loss, seriously affect agrological productivity, make crop yield with
Quality decline also brings harm to human body in addition, noxious material is into after people's food chain, and the production of domestic soil conditioner at present
Product are mostly more single-minded and attached most importance to quality soil acid-base, and applying for a long time can also be raw to soil generation secondary pollution
The soil improvement of object mushroom is mostly single culture, and there is also the single problems of effect, therefore we provide a kind of new soil and change
Good dose for improving current status.
Summary of the invention
For above situation, in order to improve serious China's soil pollution, soil hardening, salinization of soil, nutrient loss status,
The present invention provides a kind of fulvic acid biological bacteria modifying agent, and main component fulvic acid can not only the toxic huge sum of money of selective absorption
Belong to, in soil must element complex reaction can occur, increase the biological effectiveness of essential elements, and soil can be increased
Fertility, main component bacillus subtilis and bacillus megaterium can be good at adaptability, cooperate, can be good at
Pesticide Residue in Soil organic substance residues are solved, remaining chemical fertilizer, improves Soil Respiration in soil of degrading, and improves soil physical chemistry
The problem of matter, comprehensive treatment soil pollution, will not generate secondary phytotoxicity.
Specific technical solution is as follows:
A kind of fulvic acid biological bacteria modifying agent, which is characterized in that fulvic acid biological bacteria modifying agent is by fulvic acid, bacillus subtilis
Bacterium, bacillus megaterium composition, the quality accounting of fulvic acid, bacillus subtilis, bacillus megaterium in formula are 3%,
10%, 10%, surplus is water.
A kind of preparation method another object of the present invention is to provide fulvic acid biological bacteria as soil conditioner,
The fulvic acid biological bacteria modifying agent as to basic soil improve the preparation method comprises the following steps:
(1) 80g fulvic acid is dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure, stirs 10min, obtain PH 5-6
Acid solution adsorb harmful huge sum of money element in soil, while also providing the institute that survives for biological bacteria in preparation for adjusting PH
Need nutrition；
(2) (1) is obtained that 20,000,000,000/g of full water-soluble bacillus subtilis 100g is added in solution, room temperature, is stirred under an atmospheric pressure
Mix dissolution 10min.It is added and is used for bacillus subtilis, the organic contamination in soil can be removed, promote residual urea in soil
And the absorption of application urea, while can also improve the quantity and activity of the probiotics in soil；
(3) 20,000,000,000/g full water-soluble bacillus megaterium 100g, room temperature, an atmospheric pressure will be added in solution obtained by (2)
Lower stirring and dissolving 10min.Bacillus megaterium is added, there can be the decomposition of insoluble phosphorus in contact soil, increase soil fertility, together
When can be further improved due to the presence of bacillus subtilis, the activity of bacillus megaterium；
(4) solution made from (3) is added to the stirring of 50g glucose under room temperature, an atmospheric pressure, dissolves 10min.Grape is added
Sugar further can provide certain nutrition foundation for the biological bacteria in formula, guarantee the survival of biological bacteria in solution.
A kind of preparation method another object of the present invention is to provide fulvic acid biological bacteria as soil conditioner,
The fulvic acid biological bacteria modifying agent as to acid soil improvement the preparation method comprises the following steps:
(1) 50g potassium carbonate is dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure, 10min is stirred, until completely molten
Solution；
(2) 80g fulvic acid will be added in solution made from (1) to be dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure,
30min is stirred, the alkaline solution of PH 9-10 is obtained, for adjusting P in soil H, adsorbs harmful huge sum of money element in soil, simultaneously
Also nutrition needed for providing existence for biological bacteria in preparation；
(3) (2) are obtained that 20,000,000,000/g of full water-soluble bacillus subtilis 100g is added in solution, room temperature, is stirred under an atmospheric pressure
Mix dissolution 10min.It is added and is used for bacillus subtilis, the organic contamination in soil can be removed, promote residual urea in soil
And the absorption of application urea, while can also improve the quantity and activity of the probiotics in soil；
(4) 20,000,000,000/g full water-soluble bacillus megaterium 100g, room temperature, an atmospheric pressure will be added in solution obtained by (3)
Lower stirring and dissolving 10min.Bacillus megaterium is added, there can be the decomposition of insoluble phosphorus in contact soil, increase soil fertility, together
When can be further improved due to the presence of bacillus subtilis, the activity of bacillus megaterium；
(5) solution made from (2) is added to the stirring of 50g glucose under room temperature, an atmospheric pressure, dissolves 10min.Grape is added
Sugar further can provide certain nutrition foundation for the biological bacteria in formula, guarantee the survival of biological bacteria in solution.
Preferably, the fulvic acid biological bacteria is as the withered grass bud in the preparation method to acid soil improving agent, being related to
Spore bacillus content is 20,000,000,000/g, full soluble powder；Bacillus megaterium 20,000,000,000/g of content, full water-soluble pulvis；Glucose contains
Amount 96%；Fulvic acid acid content 50%；Carbonic acid potassium content 98%.
Preferably, application of the fulvic acid biological bacteria as soil conditioner, in 15-25 DEG C of annual outdoor temperature, plough
Make the phase, 100g-150g/ mus, to be watered 10-15kg, uniformly spray.
(1) present invention provides a kind of fulvic acid biological bacteria modifying agent, relative to conventional soil modifying agent, in this preparation in richness
Acid is the main component of the soil organism, small to soil pollution, and bacillus subtilis, bacillus megaterium are biological agent to ring
Border generates secondary pollution；Organic contamination in soil can be effectively treated in this preparation；This preparation can effectively solve in soil because of mistake
The indissoluble phosphorus that amount is deposited in the soil using phosphate fertilizer potash fertilizer solves because asking for soil hardening caused by phosphate fertilizer potash fertilizer is excessively used
Topic；
(2) the fulvic acid biological bacteria modifying agent can be improved utilization rate of fertilizer, reduce fertilizer compared to conventional soil modifying agent
Usage amount and the respiration that can be improved soil, choosing can be had by improving the gas permeability of soil compared to conventional soil modifying agent
Selecting property adsorbs harmful heavy metal ions, reduces harmful heavy metal ions biological effectiveness, improves the physicochemical property in salt-soda soil, and
Secondary phytotoxicity will not be generated.
Specific embodiment
For the present invention aforementioned and other technology contents, feature and effect will in the detailed description of following embodiment
It can clearly present.
The present invention provides a kind of fulvic acid biological bacteria modifying agent, and ingredient fulvic acid quality accounting 3% in formula is being matched
Fang Zhongwei exists mainly as the form of huge sum of money adsorbent harmful in soil, while providing needed for growth for biological bacteria in formula
Nutrition improves biological bacteria survival, and fulvic acid is agron chief component, and humus is the main part of the soil organism
Point, it is dark, heterogeneous compound, is in the soil often in the reunion shape of different tightness degrees from soil mineral part
State forms various stable organic-mineral compounds or composite colloid, can be divided into humic acid (HA), fulvic acid (FA) and Hu Min
Plain (HM), is the important component of soil texture, wherein humic acid, fulvic acid, fertilizer conservation and nutrient supplying capability qualitative correlation with soil,
And the growth and development of crop is influenced, for fulvic acid aqueous solution in acidity, it can be dissolved in water, can also be dissolved in bronsted lowry acids and bases bronsted lowry, while it
Also have the characteristics that low molecular weight and high activity, therefore it can paste well and merge minerals and element to its molecule knot
In structure, possess good dissolubility and mobility, and the humic acid namely main component of common humic acid currently on the market,
Its molecular weight is big, and property is active not as good as fulvic acid, while it is not soluble in water, is dissolved in alkaline solution, therefore is in the market mostly it
Monovalence sylvite or sodium salt, secondly plus salt precipitated with the generation how in connection of high price salt, although can play administer it is heavy metal-polluted
The effect of dye but also cause such as calcium ion, iron ion, magnesium ion to be fixed simultaneously to reduce fertilizer efficiency, but fulvic acid its
Monovalent ion or divalent ion can be complexed to form stable complex compound and be absorbed by plants with it, and research shows that in richness
Acid can effectively reduce its bioactivity to having preferable adsorptivity in the heavy metals such as lead ion, cadmium ion.
Its ingredient bacillus subtilis quality accounting 10% in formula, is a kind of multifunctional bio bacterial manure, micro- to people and animals
Poison, no pollution to the environment, to crop safety, strong applicability, bacillus subtilis acts in soil and can solve: absorption degradation
Polycyclic aromatic hydrocarbons (PAH) in soil has the function that handle Pesticide-Polluted Soil organic contamination；Have strongly to the respiration of soil
Stimulation, improve the gas permeability of soil；The activity of soil urease in soil is improved to have the function that fixed nitrogen；Improve saline and alkaline
The physicochemical property on ground.
Its ingredient bacillus megaterium quality accounting 10% in formula is that a kind of phosphorus decomposing promotees potassium bacterium, and China arable land is most of
Lack phosphorus potassium deficiency, to improve agricultural production a large amount of application phosphorus potash fertilizers in the past, but phosphate fertilizer bury after due to leaching loss, volatilization and Fe3+、
Al3+、Mg2+It is that crop cannot absorb etc. the phosphate for forming indissoluble, leads to phosphate fertilizer, potassium utilization rate decline, and because change
The long-time service for learning fertilizer leads to soil hardening, to influence the product and quality of farming, bacillus megaterium passes through in crop
Rhizosphere colonization, using metabolism organic acid is by fixed phosphorus in the soil and potassium is converted into rapid available phosphorus and available potassium works, mention
High soil fertility reduces the usage amount of chemical fertilizer, solves the problems, such as soil hardening.
Based on the above inventive concept, the present invention provides a kind of preparation method of fulvic acid biological bacteria modifying agent.
Embodiment one, fulvic acid biological bacteria modifying agent as to basic soil improve the preparation method comprises the following steps:
(1) 80g fulvic acid is dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure, stirs 10min, obtain PH 5-6
Acid solution adsorb harmful huge sum of money element in soil, while also providing the institute that survives for biological bacteria in preparation for adjusting PH
Need nutrition；
(2) (1) is obtained that 20,000,000,000/g of full water-soluble bacillus subtilis 100g is added in solution, room temperature, is stirred under an atmospheric pressure
Mix dissolution 10min.It is added and is used for bacillus subtilis, the organic contamination in soil can be removed, promote residual urea in soil
And the absorption of application urea, while can also improve the quantity and activity of the probiotics in soil；
(3) 20,000,000,000/g full water-soluble bacillus megaterium 100g, room temperature, an atmospheric pressure will be added in solution obtained by (2)
Lower stirring and dissolving 10min.Bacillus megaterium is added, there can be the decomposition of insoluble phosphorus in contact soil, increase soil fertility, together
When can be further improved due to the presence of bacillus subtilis, the activity of bacillus megaterium；
(4) solution made from (3) is added to the stirring of 50g glucose under room temperature, an atmospheric pressure, dissolves 10min.Grape is added
Sugar further can provide certain nutrition foundation for the biological bacteria in formula, guarantee the survival of biological bacteria in solution.
It can be used as the use of alkaline soil improver by above step obtained solution.
Embodiment two, fulvic acid biological bacteria modifying agent as to acid soil improvement the preparation method comprises the following steps:
(1) 50g potassium carbonate is dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure, 10min is stirred, until completely molten
Solution；
(2) 80g fulvic acid will be added in solution made from (1) to be dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure,
30min is stirred, the alkaline solution of PH 9-10 is obtained, for adjusting P in soil H, adsorbs harmful huge sum of money element in soil, simultaneously
Also nutrition needed for providing existence for biological bacteria in preparation；
(3) (2) are obtained that 20,000,000,000/g of full water-soluble bacillus subtilis 100g is added in solution, room temperature, is stirred under an atmospheric pressure
Mix dissolution 10min.It is added and is used for bacillus subtilis, the organic contamination in soil can be removed, promote residual urea in soil
And the absorption of application urea, while can also improve the quantity and activity of the probiotics in soil；
(4) 20,000,000,000/g full water-soluble bacillus megaterium 100g, room temperature, an atmospheric pressure will be added in solution obtained by (3)
Lower stirring and dissolving 10min.Bacillus megaterium is added, there can be the decomposition of insoluble phosphorus in contact soil, increase soil fertility, together
When can be further improved due to the presence of bacillus subtilis, the activity of bacillus megaterium；
(5) solution made from (2) is added to the stirring of 50g glucose under room temperature, an atmospheric pressure, dissolves 10min.Grape is added
Sugar further can provide certain nutrition foundation for the biological bacteria in formula, guarantee the survival of biological bacteria in solution.
Fulvic acid biological bacteria modifying agent is as in the preparation method to acid soil improvement, related bacillus subtilis
Content is 20,000,000,000/g, full soluble powder；Bacillus megaterium 20,000,000,000/g of content, full water-soluble pulvis；Glucose content 96%；
Fulvic acid acid content 50%；Carbonic acid potassium content 98%, the solution as made from above step can be used as making for acid soil improving agent
With.
This fulvic acid biological bacteria modifying agent is aqua, and specifically used method is: in 15-25 DEG C of annual outdoor temperature, arable land
Plough is made the phase, 100g-150g/ mus, to be watered 10-15kg, uniformly spray.
The present invention provides a kind of fulvic acid biological bacteria modifying agent, relative to conventional soil modifying agent, fulvic acid in this preparation
Small to soil pollution for the main component of the soil organism, bacillus subtilis, bacillus megaterium are biological agent to environment
Generate secondary pollution；Organic contamination in soil can be effectively treated in this preparation；This preparation can effectively solve in soil because of excess
The indissoluble phosphorus deposited in the soil using phosphate fertilizer potash fertilizer is solved because asking for soil hardening caused by phosphate fertilizer potash fertilizer is excessively used
Topic；
Utilization rate of fertilizer can be improved compared to conventional soil modifying agent in the fulvic acid biological bacteria modifying agent, reduces making for fertilizer
Dosage and the respiration that can be improved soil, selection can be had by improving the gas permeability of soil compared to conventional soil modifying agent
Property absorption harmful heavy metal ions, reduce harmful heavy metal ions biological effectiveness, improve the physicochemical property in salt-soda soil, and not
Secondary phytotoxicity can be generated.
It is described above simply to illustrate that of the invention, it is understood that the present invention is not limited to the above embodiments, meets this hair
The various variants of bright thought are within the scope of the present invention.
Claims (5)
1. a kind of fulvic acid biological bacteria modifying agent, which is characterized in that fulvic acid biological bacteria modifying agent is by fulvic acid, bacillus subtilis
Bacterium, bacillus megaterium composition, the quality accounting of fulvic acid, bacillus subtilis, bacillus megaterium in formula are 3%,
10%, 10%, surplus is water.
2. a kind of a kind of preparation method of fulvic acid biological bacteria modifying agent described in claim 1, which is characterized in that in the richness
Sour biological bacteria modifying agent as to basic soil improve the preparation method comprises the following steps:
(1) 80g fulvic acid is dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure, stirs 10min, obtain PH 5-6
Acid solution adsorb harmful huge sum of money element in soil, while also providing the institute that survives for biological bacteria in preparation for adjusting PH
Need nutrition；
(2) (1) is obtained that 20,000,000,000/g of full water-soluble bacillus subtilis 100g is added in solution, room temperature, is stirred under an atmospheric pressure
Dissolution 10min is mixed, is added and is used for bacillus subtilis, the organic contamination in soil can be removed, promotes residual urea in soil
And the absorption of application urea, while can also improve the quantity and activity of the probiotics in soil；
(3) 20,000,000,000/g full water-soluble bacillus megaterium 100g, room temperature, an atmospheric pressure will be added in solution obtained by (2)
Bacillus megaterium is added in lower stirring and dissolving 10min, can have the decomposition of insoluble phosphorus in contact soil, increase soil fertility, together
When can be further improved due to the presence of bacillus subtilis, the activity of bacillus megaterium；
(4) solution made from (3) is added to the stirring of 50g glucose under room temperature, an atmospheric pressure, dissolves 10min, grape is added
Sugar further can provide certain nutrition foundation for the biological bacteria in formula, guarantee the survival of biological bacteria in solution.
3. a kind of a kind of preparation method of fulvic acid biological bacteria modifying agent described in claim 1, which is characterized in that in the richness
Sour biological bacteria modifying agent as to acid soil improvement the preparation method comprises the following steps:
(1) 50g potassium carbonate is dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure, 10min is stirred, until completely molten
Solution；
(2) 80g fulvic acid will be added in solution made from (1) to be dissolved in the reaction kettle of 1L, at normal temperature, under an atmospheric pressure,
30min is stirred, the alkaline solution of PH 9-10 is obtained, for adjusting P in soil H, adsorbs harmful huge sum of money element in soil, simultaneously
Also nutrition needed for providing existence for biological bacteria in preparation；
(3) (2) are obtained that 20,000,000,000/g of full water-soluble bacillus subtilis 100g is added in solution, room temperature, is stirred under an atmospheric pressure
Dissolution 10min is mixed, is added and is used for bacillus subtilis, the organic contamination in soil can be removed, promotes residual urea in soil
And the absorption of application urea, while can also improve the quantity and activity of the probiotics in soil；
(4) 20,000,000,000/g full water-soluble bacillus megaterium 100g, room temperature, an atmospheric pressure will be added in solution obtained by (3)
Bacillus megaterium is added in lower stirring and dissolving 10min, can have the decomposition of insoluble phosphorus in contact soil, increase soil fertility, together
When can be further improved due to the presence of bacillus subtilis, the activity of bacillus megaterium；
(5) solution made from (2) is added to the stirring of 50g glucose under room temperature, an atmospheric pressure, dissolves 10min, grape is added
Sugar further can provide certain nutrition foundation for the biological bacteria in formula, guarantee the survival of biological bacteria in solution.
4. a kind of preparation method of fulvic acid biological bacteria modifying agent according to claim 3, which is characterized in that described system
Bacillus subtilis bacterial content involved in Preparation Method is 20,000,000,000/g, full soluble powder；Bacillus megaterium 20,000,000,000/g of content, entirely
Water solube powder；Glucose content 96%；Fulvic acid acid content 50%；Carbonic acid potassium content 98%.
5. a kind of a kind of application of fulvic acid biological bacteria modifying agent described in claim 1, which is characterized in that in annual outdoor temp
15-25 DEG C of degree, plough is made the phase, 100g-150g/ mus, to be watered 10-15kg, uniformly spray.
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| CN110922253A (en) * | 2019-12-19 | 2020-03-27 | 上海市园林科学规划研究院 | Magnolia denudata rhizosphere soil active agent and preparation method and application thereof |
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Application publication date: 20190517 |