CN103467144A - Refined fulvic-acid-containing agricultural microbial inoculum and production method thereof - Google Patents
Refined fulvic-acid-containing agricultural microbial inoculum and production method thereof Download PDFInfo
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Abstract
The invention discloses a refined fulvic-acid-containing microbial inoculum and a production method thereof, and relates to the microbial inoculum using biological humic acid and functional bacteria as main raw materials and the production method thereof. The refined fulvic-acid-containing microbial inoculum is prepared by mixing 75 to 80 parts by weight of biological humic acid powder, 10 to 15 parts by weight of an active humic acid liquid, 1.5 parts by weight of bacillus subtilis microbial inoculum and 8.5 parts by weight of a binder, and is a fine grained product with the moisture content of 12%-15%. In the product, organic matters account for 56-58% of the total dry matter weight of the product, fulvic acid in the organic matters accounts for 15.5-16.2% of the total dry matter weight of the product, the living bacterium quantity of bacillus subtilis is more than or equal to 2*108/g, and the fine grained shape means that the weight of fine grains with the size of 0.5-2 mm in the product accounts for more than 80% of the total weight of the product. In the product, the fulvic acid content is high, the functional bacteria content is rich, the fine grained agent form is good, the use amount is low, and the product is suitable for various application modes, all crops and cultivated lands, and has very significant effects on improvement of soil and promotion of production increasing.
Description
Technical field
The present invention relates to a kind of take that biological humic and function yeast be main raw material containing humic acid agricultural microbiobacterial agent and manufacture method thereof.
Background technology
Biological humic is collective's innovation achievement of a large amount of scientific workers of China beginning of this century last century Mo, to transform with the microbial technique utilization organic active substance that organic waste forms, its most important effective ingredient is xanthohumic acid (FA), most solid (powdery) biological humics also contain function yeast (B), so this class humic acids is abbreviated as again BFA.This professional term of biological humic and product class have announced definite in China Humic Acid TIA in 2007 annual meeting (Beijing), and have done reflection in the academic documents such as " humic acids application book series " published and distributed in chemical industry press.
Recent two decades comes, the biological humic product mainly circulates in agricultural materials market with " starter " of powdery and aqueous " fertilizer for spraying on leaf surface " two kinds of forms, powder, liquid divide two lines, every the line application surface is all narrow, restrict the large-scale promotion application of this series products, also be difficult to the support and the transformation that make agriculture field obtain the biological humic technical spirit.
Our company obtained two patents of invention (ZL03138767.5 and ZL03112360.0) once, production and selling biological humic pulvis and activated humic acid liquid for many years, and these two kinds of products are to improvement soil and improve crop yield unusual effect is arranged.But the biological humic powder, except having aspect doing the organic fertilizer fermentation agent good market response, because it is very light finely powdered, is used inconvenience, be directly used in that farmland is more difficult pushes open.Activated humic acid liquid sprays for the manufacture of fertilizer for spraying on leaf surface, and each consumption is restricted, limited to the effect of increasing production of crop, and the more difficult expansion in water-deficient area, so consumption is hovered at low level always.
20 years (1990-2010) that the biological humic technology is firstly appeared, the insider continues to use mineral humic acids theory, thereby only fertilizer for spraying on leaf surface and common fertilizer manufacture two aspect applying biological humic acids product, although having demonstrated the biological humic product has superiority than mineral humic acids, but can not form all the time the upper large-scale application to the biological humic product of agricultural, and the biological humic insider is in the face of the organic waste of annual billions of tons (dry-matter), also just can only be on small scale, wonderful works can not be arranged.Because the market consumption of the main product for agriculture of above-mentioned two class has limited the large-scale application to the biological humic product.
Summary of the invention
The present invention aims to provide that a kind of refining its consumption is few, effect is strong containing xanthohumic acid agricultural microorganism microbial inoculum, is applicable to various farmlands and uses; And provide the manufacture method of this agricultural microorganism microbial inoculum.
Technical scheme of the present invention is: a kind of refining containing xanthohumic acid agricultural microorganism microbial inoculum, it is the fine-grannular that is mixed of the cakingagent by the bacillus subtilis microbial agent of the activated humic acid liquid of the biological humic powder of 75-80 weight part, 10-15 weight part, 1.5 weight parts and 8.5 weight parts and the product of water ratio 12%-15%, and the weight that in this product, effective ingredient has weight to account for xanthohumic acid in organic and this organic matter of this product dry-matter gross weight 56-58% accounts for the viable bacteria amount of this product dry-matter gross weight 15.5%-16.2%, subtilis>=2 * 10
8individual/gram; So-called fine-grannular product refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter; The fineness of biological humic powder wherein is at 60-80 order and water ratio 9%-10%, and the weight that has weight to account for xanthohumic acid in organic and this organic matter of its gross weight 65%-67% in this biological humic dried bean noodles material accounts for the 15.5%-17% of this biological humic dried bean noodles material gross weight; Activated humic acid liquid water ratio 45%-50% wherein, the weight that has weight to account for xanthohumic acid in organic and this organic matter of its gross weight 25%-27% in this activated humic acid liquid accounts for the 18%-20% of this activated humic acid liquid gross weight; Cakingagent wherein is that fineness is at 60-80 purpose granulation of fertilizer clay; The viable bacteria amount of bacillus subtilis microbial agent wherein>=2 * 10
10individual/gram.
The above-mentioned refining manufacture method containing xanthohumic acid agricultural microorganism microbial inoculum, the step comprised has:
Step 1, pre-mixing; The cakingagent of the activated humic acid liquid of the biological humic powder of 75-80 weight part, 10-15 weight part and 8.5 weight parts is added in two ribbon mixers and mixes 5-10 minute, make Preblend; The fineness of biological humic powder wherein is at 60-80 order and water ratio 9%-10%, and the weight that has weight to account for xanthohumic acid in organic and this organic matter of its gross weight 65%-67% in this biological humic dried bean noodles material accounts for the 15.5%-17% of this biological humic dried bean noodles material gross weight; Activated humic acid liquid water ratio 45%-50% wherein, the weight that has weight to account for xanthohumic acid in organic and this organic matter of its gross weight 25%-27% in this activated humic acid liquid accounts for the 18%-20% of this activated humic acid liquid gross weight; Wherein cakingagent is that fineness is at 60-80 purpose granulation of fertilizer clay;
Step 2, die mould and fragmentation; Above-mentioned Preblend is added in rolling-type tablets press or rotating-expressing type squeezing machine and is pressed into particle or flap, above-mentioned particle or flap are added in the pawl formula crusher of not being with screen cloth and be cut into the fine-grannular woollen, and in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter;
Step 3, secondary mixes; By the viable bacteria amount of above-mentioned fine-grannular woollen and 1.5 weight parts>=2 * 10
10the bacillus subtilis microbial agent of individual/gram is put into tipping bucket type mixing machine mixing 5-10 minute, make the product of fine-grannular and water ratio 12%-15%, the weight that in this product, effective ingredient has weight to account for xanthohumic acid in organic and this organic matter of this product dry-matter gross weight 56-58% accounts for the 15.5%-16.2% of this product dry-matter gross weight, the viable bacteria amount of subtilis>=2 * 10
8individual/gram.
Method of the present invention is mixed biological humic powder (BFA) (mixing machine can adopt two ribbon mixers) with activated humic acid liquid and appropriate tamanori (clay); by the roll extrusion granulator granulation; or make sheet through the rotating-expressing type squeezing machine; again through not with the pawl formula crusher in crushing beading shape of screen cloth; then mix with bacillus subtilis microbial agent at the tipping bucket type mixer, just can pack sale.The course of processing is dry-type processing, exempts from drying or airing, has retained the physiologically active of humic acids, makes the function yeast in starting material be able to whole reservations.
Method of the present invention, by biological humic powder, activated humic acid liquid " two bar line " into a line, has also added the bacterium bacillus subtilis microbial agent of high bacterium amount to produce containing xanthohumic acid agricultural microorganism microbial inoculum simultaneously.This invention will make product reach the product characteristics of " fine-grannular ", makes these two kinds of major ingredients of biological humic powder and activated humic acid liquid fully fused under the help of clay, can conveniently use again, conveniently rapid permeability in soil simultaneously.But also do not have at present suitable machinery to can be used to directly manufacture the fines (dry type granulation) of 0.5-2 millimeter, therefore method of the present invention is selected first dry type roll extrusion or spinning, output macrobead or blade, then be broken into the fines of 0.5-2 millimeter.
Design of the present invention derives from the contriver in recent years to the theory innovation of biological humic subject.Over the past two years, contriver's lot of experiments and put into practice shocking, reasonable disposition biological humic powder, activated humic acid liquid, and combine with the function yeast subtilis, produce incredible effect in the farm crop application: every mu every batch crop is used 7.5-10KG, larger than the control group of same water and fertilizer management volume increase 15-50%(inhomogeneity crop diversity ratio), in the situation that soil-borne disease or natural disaster occur, stimulation ratio is higher, and crops quality is better.Utilize the said products to mix use with chemical fertilizer, or spread manuer in holes separately, or mix water-soluble watering and execute, can produce the effect of using bio-organic fertilizer, but equal its consumption of effect is only 10% left and right of general biological organic fertilizer.Every batch of emergency crops is only used once, and crop is used 2-3 time every year throughout the year, just can play improvement soil, improves chemical fertilizer utilization ratio, increase the effect of output and lifting crop quality.Through many places, application result proves, uses 10KG for every mu at every turn, and various crop yields are as follows: piece root tuber crops volume increase 30-40%; Melon eggplant green pepper beans volume increase 25-30%; Leaf vegetables volume increase 20-25%; Fruits volume increase 10-15%; Standing grain section grain class stimulation ratio is higher; In the land use of continuous cropping, soil-borne disease, or drought, waterlogging occur, the disaster such as freeze, increase production more than 50% on year-on-year basis.Adhere to using product of the present invention, soil can continue farming forever, and this is significant to agricultural modernization.With a large amount of microbial inoculums of independent application or apply separately humic acids and compare, the result of use of said mixture is also obviously much better again.This recognizes us: continue to use mineral humic acids theoretical (absorption, chelating, complexing, cation replacement etc.), can't explain above biological humic application phenomenon, the biological humic subject must be founded the theory and technology system of oneself.
Below the theoretical frame of our Primary Construction:
Which type of fertilizer should one, we manufacture?
The fertilizer manufacturing process of traditional classical is repeatedly turning of aerobic fermentation, hyperthermia drying, produce " mineralising soil ulmin ", this technique is converted in a large number Carbon emission to the carbon in material and falls, the microorganism that fermenting process produces also exhausts, fertilizer has lost its core value---and activated humic acid and microorganism, this fertilizer is soil improvement agent at most.
Does two, why soil harden, and is that " using in a large number chemical fertilizer " causes?
We find wetland or the river bank at some edges, farmland, and the content of chemical fertilizer is also higher than near soil content, but many earthworms are arranged, and this explanation earthworm " is not poisoned with poison " by chemical fertilizer, but is died of hunger or starve and run.Because soil organic matter content is too low, earthworm has not had food.In like manner, soil with organic matter very little, makes carbon-nitrogen ratio too low, and microorganism is irreproducible, so soil compaction.Toward soil, add water-soluble organic matter (being rich in activated humic acid) just can make soil reappear activity as seen, the main points of the biological humic fertilizer mechanism of action that Here it is it
Three, use the ferment-fermented fertilizer of biological humic class can not turning why, thereby produce the Organic fertilizer that is rich in activated humic acid and microorganism?
Comprise the multiclass bacterium because the biological humic production process is natural, aerobic, facultative and anerobe, and humic acids provides the initial energy to microorganism, the metabolism of microorganism produces again xanthohumic acid, both are in conjunction with constantly promoting mutually cyclic extensions under certain condition, and this is the relation of " egg white " and " yolk ", is 1+1>2, this makes not turning be fermented into possibility, is also the reason that makes the soil quick improvement.
Four, can not explain by the theory of " chemical fertilizer fertilising " production-increasing function of product of the present invention.
From the mathematical logic Epidemiological Analysis, the growth effect that a kind of factor causes is the relation of linear growth, the growth effect that two kinds of factors cause is exactly the relation with increase of binary or quadratic power, and the growth effect that the multidimensional and multiple-level face factor " general mobilization " causes will produce the phenomenon of similar geometricprogression.Therefore can not explain by the theory of " chemical fertilizer fertilising " Increase production ability of product of the present invention.The use of product of the present invention can improve three kinds of fertility of soil simultaneously: physics fertility, Chemical fertility (promoting carbon " short slab ") and biological fertility, the lifting of these three kinds of fertility interacts again and promotes mutually, thereby causes the especially big lifting to the crop yield quality.Metaphor by a kind of image: " trigger electrode " of the similar triode of product of the present invention (or controlling the utmost point), just can get through the macro-energy passage to a little energy.
Is five, what the basis of biological humic theory?
China's fertilizer, the biological organic fertilizer industry very long winding raod of passing by, still there is major defect in current production technique so far, and its reason is mainly the misleading of disappearance or the old theory of new theory.The fertilizer scientific worker should catch these two fertile core---water-soluble organic matter (activated humic acid) and functional microorganisms of planting, carry out technology and process innovation, could draw upper correct road to the development of this two classes fertilizer, this should be also the basis of biological humic theory.
The present invention make with extra care containing xanthohumic acid agricultural microorganism microbial inoculum, biological humic powder, activated humic acid liquid are added to the pure function bacterium bacillus subtilis microbial agent three use that is blended together, confirm through a large amount of field tests: this is a kind of practicality, have the biological organic fertilizer function and consumption is only " containing xanthohumic acid agricultural microorganism microbial inoculum " of ordinary student organic fertilizer 10% left and right.This fertilizer xanthohumic acid content is high, functional bacterial content abundant, physical behavior is fine-grannular, and formulation is good, consumption is few, is adapted to various methods of application: can be mixed with chemical fertilizer, also can use separately; Can directly spread fertilizer over the fields, also miscible watering in water executed, rushed and execute.Being applicable to all crops and all places, is a kind of concentrated type biological organic fertilizer kind of conveniently using popularization.For example:
One, paddy rice application test
Time: 2011.7.20-2011.11.15;
Experiment material: test group enriches refining containing every mu of 10KG of xanthohumic acid agricultural microorganism microbial inoculum, and other water and fertilizer managements and control group are together.
Experimental result is as following table
Discuss: from test-results, add refiningly containing xanthohumic acid agricultural microorganism microbial inoculum group, paddy thousand seed weight increases, and commodity paddy stimulation ratio is higher than gross weight stimulation ratio, illustrate that in paddy, substandard products are few, this all reflect paddy not only output increase, and quality raising.
Two, balsam pear application test
Time: 2011.9.5-2012.1.10
Experiment material: test group enriches refining containing every mu of 10KG of xanthohumic acid agricultural microorganism microbial inoculum, and other water and fertilizer managements and control group are together.
Experimental result is as following table
Discuss: from test-results, add and refining extend 16 days containing the xanthohumic acid agricultural microorganism microbial inoculum group collecting period, and production unit cost is higher than control group, be reflected in the rate of increasing income higher than stimulation ratio.
Three, leafiness Chinese cabbage application practice
Time: 2011.12.26-2012.2.2
Experiment material: A organizes pure subtilis (2 * 10
10individual/g) every mu of 8.3KG; B group refining containing every mu of 8.3KG of xanthohumic acid agricultural microorganism microbial inoculum; Every mu of 8.3KG of C group biological humic powder; The CK group is above every for not adding, and other water and fertilizer managements are respectively organized identical.
Experimental result is as following table
| Group | Per mu yield (KG) | Increase production (KG) with CK than mu | Compare stimulation ratio with CK |
| A | 3618.9 | 223.8 | 6.6% |
| B | 4175.0 | 779.9 | 23.0% |
| C | 3757.5 | 362.4 | 10.6% |
| CK | 3395.1 | ---- | ---- |
Result proves: three kinds of material (pure function bacterium 2 * 10
10individual/g, refining containing xanthohumic acid agricultural microorganism microbial inoculum and biological humic powder) effect of increasing production is arranged, is arranged as with effect size (effect):
Refining containing humic acid agricultural microbiobacterial agent>biological humic powder>pure function bacterium 2 * 10
10individual/g.
Four, Caulis et Folium Brassicae capitatae application experiment
Time: 2011.10.12-2012.03.05
Experiment material: A organizes every mu and is affixed by the 100KG biological organic fertilizer; B organizes every mu and is affixed by the 150KG biological organic fertilizer; Every mu of C group is affixed by refining containing xanthohumic acid agricultural microorganism microbial inoculum 10KG; The CK group is not for adding.Water and fertilizer management is respectively organized identical, and every mu is used 16-16-16 unexpected rival's board composite fertilizer 100KG to make base manure.
Experimental result is as following table
Discuss: from the table result: on every mu of basis of using 100KG composite fertilizer, add fertilizer and refining all increasing production to some extent than comparative group containing xanthohumic acid agricultural microorganism microbial inoculum, and the volume increase size order is as follows:
The B(150KG biological organic fertilizer)>C(10KG refining containing xanthohumic acid agricultural microorganism microbial inoculum)>the A(100KG biological organic fertilizer)
Can tentatively judge thus: refining is slightly less than the biological organic fertilizer of its 15 times of amounts containing xanthohumic acid agricultural microorganism microbial inoculum effect of increasing production, and is greater than the biological organic fertilizer of its 10 times of amounts.
Five, potato application test
Time: on March 15,13 days to 2012 November in 2011
Experiment material: experimental group add every mu of 10KG refining containing xanthohumic acid agricultural microorganism microbial inoculum, control group does not add.Same every mu of base manure is executed 100KG16-16-16 unexpected rival's board composite fertilizer, and control measures are identical.
The application experiment result is as following table
Discuss: from the table result, test group (every mu adds refining containing xanthohumic acid agricultural microorganism microbial inoculum 10KG), per mu yield and the output value all have very significantly and increase than control group, from seedling rate and soil moisture content, add refining containing after xanthohumic acid agricultural microorganism microbial inoculum, soil obtains fine improvement, and this causes, and high, the qualified fruit rate of potato seedling rate is high, fruit is even, so per mu yield value rate of increase is higher than per mu yield rate of increase.From this group test, refining may be remarkable especially to the effect of increasing production of stem tuber piece root crop containing xanthohumic acid agricultural microorganism microbial inoculum.
Experimental results show that product of the present invention has very strong effect of increasing production: 10% left and right that equal effect consumption need only the ordinary student organic fertilizer, emergency crops is used 1 time, and every mu of 7.5-10KG can increase production more than 20%; Living crop is executed 2-3 time every mu of every year throughout the year, and 15-20KG, can increase production more than 15% altogether.In other words, the each amount of application of land area of one unit is also fewer than chemical fertilizer, has solved fertilizer and the bio-organic fertilizer consumption is large, and labor cost high and booth, mountain region etc. are difficult to the difficult problem of manure use and biological organic fertilizer.Therefore, product of the present invention will produce important and far-reaching impact to progress and the change of agricultural and fertilizer circle.And produce biological humic powder and the activated humic acid liquid raw material of this product, all derive from the recycling to organic waste.So product of the present invention, in the large-scale popularization application of agriculture field, will promote national environmental protection cause and carbon emission reduction greatly, catches up with and surpasses developed country for China's agricultural civilization and makes contributions.
The accompanying drawing explanation
Fig. 1 be the present invention make with extra care containing xanthohumic acid agricultural microorganism microbial inoculum product characteristics photo.
Fig. 2 is the Production Flow Chart schematic diagram containing the manufacture method of xanthohumic acid agricultural microorganism microbial inoculum that the present invention makes with extra care.
Embodiment
One, embodiment mono-
Manufacture that the present invention makes with extra care containing xanthohumic acid agricultural microorganism microbial inoculum, its specification of quality is: product is fine-grannular and water ratio 12%-15%, and the weight that in this product, effective ingredient has weight to account for xanthohumic acid in organic and this organic matter of this product dry-matter gross weight 56-58% accounts for the viable bacteria amount of this product dry-matter gross weight 15.5%-16.2%, subtilis>=2 * 10
8individual/gram; So-called fine-grannular refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter, please refer to Fig. 1.
Manufacture the process containing xanthohumic acid agricultural microorganism microbial inoculum that the present invention makes with extra care, please refer to Fig. 2.
Step 1, pre-mixing; The cakingagent of the activated humic acid liquid of the biological humic powder of 75KG, 15KG and 8.5KG is added in two ribbon mixers and mixes 5-10 minute, make Preblend.Biological humic powder in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03138767.5 patent.The water ratio of this biological humic powder is 10%, in 65% and this organic matter that in the dry-matter of this biological humic powder, organic weight is this biological humic dried bean noodles substance weight, the weight of xanthohumic acid is 15.5% of this biological humic dried bean noodles substance weight, and the fineness of this biological humic powder is at the 60-80 order.Activated humic acid liquid in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03112360.0 patent.This activated humic acid liquid water ratio 45%-50%, the weight that has weight to account for xanthohumic acid in its gross weight 25% organic matter and this organic matter in this activated humic acid liquid accounts for 18% of this activated humic acid liquid gross weight; Cakingagent wherein is that fineness is at 60-80 purpose granulation of fertilizer clay.
Step 2, die mould and fragmentation; Above-mentioned Preblend is added in the rolling-type tablets press and is pressed into particle; or the rotating-expressing type squeezing machine is extruded into sheet; above-mentioned particle or flap are added in the pawl formula crusher of not being with screen cloth and be cut into the fine-grannular woollen, and in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter.
Step 3, secondary mixes; By the viable bacteria amount of above-mentioned fine-grannular woollen and 1.5KG>=2 * 10
10the bacillus subtilis microbial agent of individual/gram is put into tipping bucket type mixing machine mixing 5-10 minute, makes granuliform finished product.Bacillus subtilis microbial agent wherein can be bought to producers such as Beihai group woods biotechnology company limiteds.
So the refining effective ingredient containing xanthohumic acid agricultural microorganism microbial inoculum of preparation is calculated as follows:
1, dry-matter:
75KG×90%+15KG×50%+8.5KG+1.5KG
=67.5KG+7.5KG+8.5KG+1.5KG=85.0KG
2, water ratio:
（75KG×10%+15KG×50%）/（75KG+15KG+8.5KG+1.5KG）
=（7.5KG+7.5KG）/100KG=15%
3, organic matter:
The contribution of biological humic powder is organic: 75KG * 90% * 65%=43.87KG
The contribution of activated humic acid liquid is organic: 15KG * 25%=3.75KG
Add up to organic: 43.87KG+3.75KG=47.62KG
Organic content in the dry-matter of product: 47.62KG/85.0KG=56%
4, xanthohumic acid:
Biological humic powder contribution xanthohumic acid: 75KG * 90% * 15.5%=10.46KG
Activated humic acid liquid contribution xanthohumic acid: 15KG * 18%=2.7KG
Add up to xanthohumic acid total amount: 10.46KG+2.7KG=13.16KG
Xanthohumic acid content: 13.16KG/85.0KG=15.5% in the product dry-matter
5, bacillus subtilis bacterial content in product:
（1.5KG×2×10
10/g）/（75KG+15KG+8.5KG+1.5KG）
=3×10
8/g
Consider product preservation period rate of fall-off, by 2 * 10
8/ g estimates, and meets the standard of GB20287-2006 " living bacteria count ".
From above calculating: the quality index that meets design code by the product of this formulated.
Two, embodiment bis-
Manufacture that the present invention makes with extra care containing xanthohumic acid agricultural microorganism microbial inoculum, its specification of quality is: product is fine-grannular and water ratio 12%-15%, and the weight that in this product, effective ingredient has weight to account for xanthohumic acid in organic and this organic matter of this product dry-matter gross weight 56-58% accounts for the viable bacteria amount of this product dry-matter gross weight 15.5%-16.2%, subtilis>=2 * 10
8individual/gram; So-called fine-grannular refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter, please refer to Fig. 1.
Manufacture the process containing xanthohumic acid agricultural microorganism microbial inoculum that the present invention makes with extra care, please refer to Fig. 2:
Step 1, pre-mixing; The cakingagent of the activated humic acid liquid of the biological humic powder of 80KG, 10KG and 8.5KG is added in two ribbon mixers and mixes 5-10 minute, make Preblend.Biological humic powder in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03138767.5 patent.The water ratio of this biological humic powder is 9%, in this biological humic dried bean noodles material organic weight account for this biological humic dried bean noodles substance weight 67%, the weight of xanthohumic acid accounts for 17% of this biological humic dried bean noodles substance weight in this organic matter, the fineness of this biological humic powder is at the 60-80 order.Activated humic acid liquid in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03112360.0 patent.This activated humic acid liquid water ratio is 45%, in this activated humic acid liquid organic weight account for its gross weight 27%, the weight of xanthohumic acid accounts for 20% of its gross weight in this activated humic acid liquid; Cakingagent wherein is that fineness is at 60-80 purpose granulation of fertilizer clay.
Step 2, die mould and fragmentation; Above-mentioned Preblend is added in rolling-type tablets press granulation or rotating-expressing type squeezing machine and is pressed into flap; above-mentioned particle or flap are added in the pawl formula crusher of not being with screen cloth and be cut into the fine-grannular woollen, and in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter.
Step 3, secondary mixes; By the viable bacteria amount of above-mentioned fine-grannular woollen and 1.5KG>=2 * 10
10the bacillus subtilis microbial agent of individual/gram is put into tipping bucket type mixing machine mixing 5-10 minute, makes granuliform finished product.Bacillus subtilis microbial agent in its raw material can be bought to producers such as Beihai group woods biotechnology company limiteds.
So the refining effective ingredient containing xanthohumic acid agricultural microorganism microbial inoculum of preparation is calculated as follows:
1, dry-matter:
80KG×（100-9）%+10KG×（100-45）%+8.5KG+1.5KG
=72.8KG+5.5KG+8.5KG+1.5KG=88.3KG
2, water ratio:
（80KG×9%+10KG×45%）/（75KG+15KG+8.5KG+1.5KG）
=（7.2KG+4.5KG）/100KG=11.7%≈12%
3, organic matter:
The contribution of biological humic powder is organic: 80KG * (100-9) * 67%=48.78KG
The contribution of activated humic acid liquid is organic: 10KG * 27%=2.7KG
Add up to organic: 48.78KG+2.7KG=51.58KG
Organic content in the product dry-matter: 51.58KG/88.3KG=58.44% ≈ 58%
4, xanthohumic acid:
Biological humic powder contribution xanthohumic acid: 80KG * (100-9) * 17%=12.34KG
Activated humic acid liquid contribution xanthohumic acid: 10KG * 20%=2.0KG
Add up to xanthohumic acid total amount: 12.34KG+2.0KG=14.34KG
Xanthohumic acid content: 14.34KG/88.3KG=16.2% in the product dry-matter
5, bacillus subtilis bacterial content in product:
（1.5KG×2×10
10/g）/（80KG+10KG+8.5KG+1.5KG）
=3×10
8/g
Consider product preservation period rate of fall-off, by 2 * 10
8/ g estimates, and meets the standard of GB20287-2006 " living bacteria count ".
From above calculating: the quality index that meets design code by the product of this formulated.
Three, embodiment tri-
Manufacture that the present invention makes with extra care containing xanthohumic acid agricultural microorganism microbial inoculum, its specification of quality is: product is fine-grannular and water ratio 12%-15%, and the weight that in this product, effective ingredient has weight to account for xanthohumic acid in organic and this organic matter of this product dry-matter gross weight 56-58% accounts for the viable bacteria amount of this product dry-matter gross weight 15.5%-16.2%, subtilis>=2 * 10
8individual/gram; So-called fine-grannular refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter, please refer to Fig. 1.
Manufacture the process of refined biological organic fertilizer material of the present invention, please refer to Fig. 2:
Step 1, pre-mixing; The cakingagent of the activated humic acid liquid of the biological humic powder of 77.5KG, 12.5KG and 8.5KG is added in two ribbon mixers and mixes 5-10 minute, make Preblend.Biological humic powder in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03138767.5 patent.The water ratio 9.5% of this biological humic powder, in this biological humic dried bean noodles material organic weight account for this biological humic dried bean noodles substance weight 66%, the weight of xanthohumic acid accounts for 16% of this biological humic dried bean noodles substance weight in this organic matter, the fineness of this biological humic powder is at the 60-80 order.Activated humic acid liquid in its raw material can be bought to Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov., or with reference to the manufacture of ZL03112360.0 patent.This activated humic acid liquid water ratio 48%, in this activated humic acid liquid organic weight account for its gross weight 26%, the weight of xanthohumic acid accounts for 19.2% of its gross weight in this activated humic acid liquid; Cakingagent wherein is that fineness is at 60-80 purpose granulation of fertilizer clay.
Step 2, die mould and fragmentation; Above-mentioned Preblend is added in rolling-type tablets press or rotating-expressing type squeezing machine and is pressed into particle or flap; above-mentioned particle or flap are added in the pawl formula crusher of not being with screen cloth and be cut into the fine-grannular woollen, and in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter.
Step 3, secondary mixes; By the viable bacteria amount of above-mentioned fine-grannular woollen and 1.5KG>=2 * 10
10the bacillus subtilis microbial agent of individual/gram is put into tipping bucket type mixing machine mixing 5-10 minute, makes granuliform finished product.Bacillus subtilis microbial agent wherein can be bought to producers such as Beihai group woods biotechnology company limiteds.
So the refining effective ingredient containing xanthohumic acid agricultural microorganism microbial inoculum of preparation is calculated as follows:
1, dry-matter:
77.5KG×（100-9.5）%+12.5KG×（100-48%）+8.5KG+1.5KG
=70.14KG+6.5KG+8.5KG+1.5KG=86.64KG
2, water ratio:
（77.5KG×9.5%+12.5KG×48%）/（75KG+15KG+8.5KG+1.5KG）
=（7.36KG+6.0KG）/100KG=13.4%≈13%
3, organic matter:
The contribution of biological humic powder is organic: 77.5KG * (100-9.5) * 66%=46.21KG
The contribution of activated humic acid liquid is organic: 12.5KG * 19.2%=2.4KG
Add up to organic: 46.21KG+2.4KG=48.61KG
Organic content in the product dry-matter: 48.61KG/86.64KG=57.08% ≈ 57%
4, xanthohumic acid:
Biological humic powder contribution xanthohumic acid: 77.5KG * (100-9.5) * 16%=11.22KG
Activated humic acid liquid contribution xanthohumic acid: 12.5KG * 19.2%=2.4KG
Add up to xanthohumic acid acid total amount: 11.22KG+2.4KG=13.62KG
Xanthohumic acid content in the product dry-matter: 13.62KG/86.64KG=15.7% ≈ 16%
5, bacillus subtilis bacterial content in product:
（1.5KG×2×10
10/g）/（77.55KG+12.5KG+8.5KG+1.5KG）
=3×10
8/g
Consider product preservation period rate of fall-off, by 2 * 10
8/ g estimates, and meets the standard of GB20287-2006 " living bacteria count ".
From above calculating: the quality index that meets design code by the product of this formulated.
The above, be only preferred embodiment of the present invention, with this, do not limit scope of the invention process, and the equivalence of doing according to technical scheme of the present invention and description changes and modifies, and all should belong to the scope that the present invention is contained.
Claims (2)
- One kind refining containing xanthohumic acid agricultural microorganism microbial inoculum, it is characterized in that: it is the fine-grannular that is mixed of the cakingagent by the bacillus subtilis microbial agent of the activated humic acid liquid of the biological humic powder of 75-80 weight part, 10-15 weight part, 1.5 weight parts and 8.5 weight parts and the product of water ratio 12%-15%, and the weight that in this product, effective ingredient has weight to account for xanthohumic acid in organic and this organic matter of this product dry-matter gross weight 56-58% accounts for the viable bacteria amount of this product dry-matter gross weight 15.5%-16.2%, subtilis>=2 * 10 8individual/gram; So-called fine-grannular product refers to that in this product, size accounts for more than 80% of this product gross weight in the particulate weight of 0.5-2 millimeter; The fineness of biological humic powder wherein is at 60-80 order and water ratio 9%-10%, and the weight that has weight to account for xanthohumic acid in organic and this organic matter of its gross weight 65%-67% in this biological humic dried bean noodles material accounts for the 15.5%-17% of this biological humic dried bean noodles material gross weight; Activated humic acid liquid water ratio 45%-50% wherein, the weight that has weight to account for xanthohumic acid in organic and this organic matter of its gross weight 25%-27% in this activated humic acid liquid accounts for the 18%-20% of this activated humic acid liquid gross weight; Cakingagent wherein is that fineness is at 60-80 purpose granulation of fertilizer clay; The viable bacteria amount of bacillus subtilis microbial agent wherein>=2 * 10 10individual/gram.
- 2. a kind of refining manufacture method containing xanthohumic acid agricultural microorganism microbial inoculum according to claim 1, the step comprised has:Step 1, pre-mixing; The cakingagent of the activated humic acid liquid of the biological humic powder of 75-80 weight part, 10-15 weight part and 8.5 weight parts is added in two ribbon mixers and mixes 5-10 minute, make Preblend; The fineness of biological humic powder wherein is at 60-80 order and water ratio 9%-10%, and the weight that has weight to account for xanthohumic acid in organic and this organic matter of its gross weight 65%-67% in this biological humic dried bean noodles material accounts for the 15.5%-17% of this biological humic dried bean noodles material gross weight; Activated humic acid liquid water ratio 45%-50% wherein, the weight that has weight to account for xanthohumic acid in organic and this organic matter of its gross weight 25%-27% in this activated humic acid liquid accounts for the 18%-20% of this activated humic acid liquid gross weight; Wherein cakingagent is that fineness is at 60-80 purpose granulation of fertilizer clay;Step 2, die mould and fragmentation; Above-mentioned Preblend is added in rolling-type tablets press or rotating-expressing type squeezing machine and is pressed into particle or flap, above-mentioned particle or flap are added in the pawl formula crusher of not being with screen cloth and be cut into the fine-grannular woollen, and in this fine-grannular woollen, size accounts for more than 80% of gross weight in the particulate weight of 0.5-2 millimeter;Step 3, secondary mixes; By the viable bacteria amount of above-mentioned fine-grannular woollen and 1.5 weight parts>=2 * 10 10the bacillus subtilis microbial agent of individual/gram is put into tipping bucket type mixing machine mixing 5-10 minute, make the product of fine-grannular and water ratio 12%-15%, the weight that in this product, effective ingredient has weight to account for xanthohumic acid in organic and this organic matter of this product dry-matter gross weight 56-58% accounts for the 15.5%-16.2% of this product dry-matter gross weight, the viable bacteria amount of subtilis>=2 * 10 8individual/gram.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105384480A (en) * | 2015-11-16 | 2016-03-09 | 北京嘉博文生物科技有限公司 | Biological humic acid organic material decomposition agent having deodorization function |
| CN109762568A (en) * | 2019-03-08 | 2019-05-17 | 河南沐天农业科技有限公司 | A kind of preparation method and applications of fulvic acid biological bacteria modifying agent |
| CN111848244A (en) * | 2019-11-03 | 2020-10-30 | 黑龙江丰富农业科技发展有限公司 | Fulvic acid type special fertilizer for industrial hemp |
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| CN1199722A (en) * | 1997-05-21 | 1998-11-25 | 王家祥 | Bacterial active humate and its production |
| CN1974490A (en) * | 2006-12-11 | 2007-06-06 | 陈五岭 | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure |
| CN101602948A (en) * | 2008-06-10 | 2009-12-16 | 丁之铨 | A kind of multifunctional soil microorganism restorative |
| CN102174583A (en) * | 2011-02-22 | 2011-09-07 | 福州大学 | Method for producing biological humic acid by mixed fermentation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1199722A (en) * | 1997-05-21 | 1998-11-25 | 王家祥 | Bacterial active humate and its production |
| CN1974490A (en) * | 2006-12-11 | 2007-06-06 | 陈五岭 | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure |
| CN101602948A (en) * | 2008-06-10 | 2009-12-16 | 丁之铨 | A kind of multifunctional soil microorganism restorative |
| CN102174583A (en) * | 2011-02-22 | 2011-09-07 | 福州大学 | Method for producing biological humic acid by mixed fermentation |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105384480A (en) * | 2015-11-16 | 2016-03-09 | 北京嘉博文生物科技有限公司 | Biological humic acid organic material decomposition agent having deodorization function |
| CN105384480B (en) * | 2015-11-16 | 2019-05-28 | 北京嘉博文生物科技有限公司 | A kind of biological humic type organic matter decomposing inoculant with deodorization functions |
| CN109762568A (en) * | 2019-03-08 | 2019-05-17 | 河南沐天农业科技有限公司 | A kind of preparation method and applications of fulvic acid biological bacteria modifying agent |
| CN111848244A (en) * | 2019-11-03 | 2020-10-30 | 黑龙江丰富农业科技发展有限公司 | Fulvic acid type special fertilizer for industrial hemp |
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Address after: 363000 Fujian Zhangzhou Zhaoan County four Zhang Gong Private Economic Zone Patentee after: FUZHOU LYUZHOU BIOCHEMICAL CO., LTD. Patentee after: Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agriculture Sciences Address before: 363000 Fujian Zhangzhou Zhaoan County four Zhang Gong Private Economic Zone Patentee before: Luzhou Bio-Chemical Co., Ltd., Zhao'an County, Fujian Prov. Patentee before: Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agriculture Sciences |