CN106278580A - A kind of Water Soluble Compound microbial manure and preparation method thereof - Google Patents
A kind of Water Soluble Compound microbial manure and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The present invention relates to a kind of Water Soluble Compound microbial manure and preparation method thereof, Water Soluble Compound microbial manure comprises bacillusmusilaginosiengineering NK BM(Bacillus mucilaginosus) and bacillus subtilis NK BS(Bacillus subtilis) two kinds of strains, and use glucose to be adjuvant to prepare through pelletize, bacillusmusilaginosiengineering NK BM(Bacillus mucilaginosus) and bacillus subtilis NK BS(Bacillus subtilis) living bacteria count ratio be 1:0.5 ~ 2 mixing, bacillusmusilaginosiengineering is 1 ~ 5 × 10 with the living bacteria count of bacillus subtilis8Cfu/g, is first mixed in proportion the zymocyte liquid of bacillusmusilaginosiengineering Yu bacillus subtilis during preparation and makes mixed bacteria liquid, then uses fluid bed granulator that mixed bacteria liquid and dextran particles are carried out fluidized granulating.Preparation method of the present invention is simple, step is easily operated, the Water Soluble Compound microbial manure prepared is suitable for multiple fertilizer application mode, as broadcasted sowing and spraying, can increase soil fertility, promotes crop yield, raising crop disease-resistant ability, soil property of improving, the usage amount of minimizing chemical fertilizer after using.
Description
Technical field
The present invention relates to a kind of Water Soluble Compound microbial manure and preparation method thereof, belong to biologic compound fertilizer and prepare skill
Art field.
Background technology
Microbial manure is that a class causes crops to obtain specific fertilizer effect with microbial life activity and metabolite thereof
The living microorganisms goods answered, are the indispensable products of China's green sustainable agriculture of development, have good using value
And economic and social benefits.At present, microbial manure product mainly has Agricultural microbial fertilizer, composite microbiological fertilizer and biology to have
Machine fertilizer three major types.Wherein composite microbiological fertilizer be two or more soil function microorganism is carried out science compounding and
Become a class bacterial manure, have during agricultural planting and preferably apply effect.Research shows, microbial manure is improving crops
Yield and quality, purifies and rehabilitating soil, soil protection ecological environment, reduces corps diseases and occurs, improves chemical fertilizer utilization ratio,
Power of fostering and apply fertilizer ground, suppression crops have important function to nitrate nitrogen, heavy metal, the aspect such as absorption of pesticide.Composite microbe fertilizer
Material is a kind of low cost, easy to use and non-harmful Green biofertilizer, is suitable for large-area promoting the use of, can be necessarily
Replacing general chemistry fertilizer in degree, to reduce, soil compaction that chemical fertilizer brings, fertility decline, environmental pollution etc. are a series of asks
Topic.Therefore, use composite microbiological fertilizer scientifically and rationally, soil environment can be improved, improve peach fruit product quality, reduce agriculture
Field is polluted, and cost-effective, economic fertilization develops eco-agriculture.
But, existing microbial manure product is mostly with the insoluble inert carrier such as the peat composed of rotten mosses, fine earth as adjuvant, and these produce
There are problems in product: (1) is only applicable to mix fertilizer or seed dressing, and not only mixing uniformity is poor, therein in actual use
Viable bacteria is also vulnerable to chemical fertilizer and seed coat agent inhibitory action, causes fertilizer efficiency to decline；(2) whipping process human cost is high, is not suitable for big
The requirement of scale plantation；(3) fertilizer application mode is single, is suitable only for broadcasting sowing, and is not useable for spraying.China's cultivated area distribution is vast,
Soil environment, weather conditions are multiformity, cause the fertilizer application mode in each area to there is significant difference, thus are unfavorable for that this type of produces
The large-scale promotion application of product.
Summary of the invention
The invention aims to solve existing microbial manure in use fertilizer efficiency poor, fertilizer application mode single and
The problem being unfavorable for large-scale promotion application, it is provided that a kind of Water Soluble Compound microbial manure and preparation method thereof, preparation side
Method easy steps is easily operated, and the Water Soluble Compound microbial manure prepared is suitable for multiple fertilizer application mode, applied range.
The present invention adopts the following technical scheme that a strain bacillusmusilaginosiengineering NK-BM (Bacillus
Mucilaginosus), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on May 4th, 2016
The heart, is called for short CGMCC, and deposit number is CGMCC No.12230.
A kind of Water Soluble Compound microbial manure, comprises bacillusmusilaginosiengineering NK-BM (Bacillus
And two kinds of strains of bacillus subtilis NK-BS (Bacillus subtilis), and to use glucose be auxiliary mucilaginosus)
Expect to prepare through pelletize, described bacillusmusilaginosiengineering NK-BM (Bacillus mucilaginosus) and bacillus subtilis NK-
The living bacteria count ratio of BS (Bacillus subtilis) is 1:0.5～2, described bacillusmusilaginosiengineering and hay spore
The living bacteria count of bacillus is 1～5 × 108cfu/g。
The preparation method of a kind of Water Soluble Compound microbial manure, comprises the steps:
(1) preparation of bacillusmusilaginosiengineering tunning: by bacillusmusilaginosiengineering bacterial strain from inclined plane inoculating to shaking flask
Activation culture 10～12h in culture medium, cultivate 12～16h in being seeded to seed tank by the inoculum concentration of 1.5～2%, by obtain
Seed liquor is seeded to produce in fermentation tank by the inoculum concentration of 1～1.2%；Fermentation condition is rotating speed 150～200r/min, dissolved oxygen ＞
10%, fermentation pH value is 6.5～7.5；Fermentation 36～48h, it is thus achieved that bacillusmusilaginosiengineering tunning, described gel-shaped spore
The bacterial content of bacillus fermentation product is 2～4 × 1010cfu/mL；
(2) preparation of bacillus subtilis (Ehrenberg) Cohn fermented product: by bacillus subtilis strain from inclined plane inoculating to shake-flask culture
Activation culture 8～12h in base, cultivate 8～12h in being seeded to seed tank by the inoculum concentration of 1.5～2% and obtain seed liquor, will plant
Sub-liquid is seeded to produce in fermentation tank by the inoculum concentration of 1～1.2%, and fermentation condition is: rotating speed 150～200r/min, dissolved oxygen ＞
8%, fermentation pH is 6.5～7.5；Fermentation 24～36h, obtains bacillus subtilis (Ehrenberg) Cohn fermented product, and described bacillus subtilis sends out
The bacterial content of ferment product is 3～5 × 1010cfu/mL；
(3) it is 1 with bacillusmusilaginosiengineering tunning by living bacteria count ratio by bacillus subtilis (Ehrenberg) Cohn fermented product:
0.5～2 mixing are compounding, make mixed bacteria liquid；
(4) use fluid bed granulator that mixed bacteria liquid and dextran particles are carried out fluidized granulating, mixed bacteria liquid and glucose
Mix than for 1:20～50 by V:W, make the fertiliser granulates that size is 1.2～4.5mm.
Further, in described step (1), the formula of Shake flask medium is: sucrose 10～15g/L, (NH4)2SO415～
20g/L、MgSO45～10g/L, K2HPO420～25g/L, yeast extract 2～5g/L, FeCl30.05～0.1g/L, CaCO3 1
～5g/L, starch 20～30g/L, initial pH value of medium is 7.0～7.5.
Further, in described step (1), the formula of the culture medium of fermentation tank is: brown sugar 2～4g/L, starch 10～15g/
L, analysis for soybean powder 1～2g/L, yeast extract 0.3～0.5g/L, (NH4)2SO42～2.5g/L, KH2PO42～5g/L, MgSO40.5～
1g/L、CaCO30.1～0.2g/L and FeCl30.01～0.05g/L, initial pH value of medium is 7.0～7.5.
Further, in described step (2), the formula of Shake flask medium is: glucose 10～15g/L, peptone 8～
10g/L, NaCl 5～10g/L, CaCO30.5～1g/L, the initial pH of culture medium is 7.0～7.5.
Further, in described step (2), the culture medium prescription of fermentation tank is: brown sugar 10～15g/L, starch 2～2.5g/
L, bean cake 12～15g/L, wheat bran 1～2g/L, yeast extract 1～2g/L, KH2PO42～3g/L, K2HPO41～2g/L, CaCO3
0.5～1g/L, MgSO40.5～1g/L, CaCl20.5～1g/L, MnSO40.2～0.5g/L, FeCl30.01～0.05g/L
With EDTA 0.01～0.1g/L, initial pH value of medium is 7.0～7.5.
Further, in described step (4), the wind pushing temperature of fluid bed granulator is 50～60 DEG C, and leaving air temp is 40～50
DEG C, air quantity is 2000～3000m3/ h, granulation time is 20～30min.
Preparation method of the present invention is simple, and step is easily operated, and the Water Soluble Compound microbial manure prepared is using
During have the beneficial effect that (1) promotes that soil w ater-stable aggregates is formed, strengthen soil organic colloid content, broken ring soil
Capillarity, effectively alleviates soil compaction, regulates the soil weight, increases porosity；Decompose the mineral phosphorus potassium silicon in soil, release
Rapid available phosphorus potassium and free silica, increase soil fertility；
(2) to the black root caused by continuous cropping, rotten, root-rot, the phenomenon such as withered, dead Seedling there is splendid effect；
(3) replace the use of a certain amount of tradition azophoska, typically can reduce the fertilizer application amount of 30～50%, strengthen
Crop autoimmunity, reduces the use of chemical pesticide, reduces crops harmful substance and agriculture is residual；
(4) produce Ecological niche competitive effect, reduce pathogen quantity；Produce aerosprin one class material, to phytopathy
Former bacterium has certain inhibitory action, can improve rhizosphere microorganism composition, reduce infection process rate；Improve the content of soil free silica,
The wax coat making plant thickens, and improves non-specific immunity and the water holding capacity of plant.Produce auxins and gibberellins
Deng material, crop is produced stimulation, promote plant growth, enhance plant resistance and nitrogen fixing capacity；
(5) applied range, can be suitable for most domestic fertilizer application mode, and pure biological preparation is nontoxic, harmless, makes
Use safe ready.
Biological material specimens preservation: a strain bacillusmusilaginosiengineering NK-BM (Bacillus mucilaginosus), protects
Being hidden in China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC, address is: Chaoyang District, Beijing City
North Star West Road 1 institute 3, Institute of Microorganism, Academia Sinica, preservation date on March 21st, 2016, deposit number is CGMCC
No.12230。
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiment one: the preparation method of a kind of Water Soluble Compound microbial manure:
(1) activated spawn
First bacillusmusilaginosiengineering NK-BM (CGMCC No.12230), bacillus subtilis NK-BS (commercially available) two are prepared
Plant the preservation inclined-plane of microorganism, with inoculating loop picking 1 ring thalline, be seeded to respectively in respective Shake flask medium, whole process
Need sterile working.By following condition activated strains:
(2) fermentation seed liquid is prepared
Use 100L fermentation system to prepare fermentation seed liquid, prepare the seed culture medium of above two microorganism respectively, add
Entering in 100L fermentation tank, liquid amount is 70%, and sterilizing is cooled to room temperature.Bacterium solution after activation is pressed the inoculum concentration of 2%, respectively
It is seeded in above-mentioned seed culture medium, cultivates to logarithmic (log) phase by respective condition of culture, make fermentation seed liquid.Two kinds of microorganisms
Seed culture medium and fermentation condition as follows:
(3) liquid fermentation
The liquid fermentation using 1t fermentation system to carry out two kinds of microorganisms produces, respectively the fermentation training of two kinds of microorganisms of preparation
Supporting base, add in 1t fermentation tank, liquid amount is 70%, and sterilizing is cooled to room temperature.By the inoculum concentration of 1.2%, by sending out of preparing
Ferment seed liquor is seeded in above-mentioned respective fermentation medium respectively, cultivates to stable phase by respective fermentation condition, collects fermentation
Liquid.The fermentation medium of two kinds of microorganisms is identical with seed culture medium, and fermentation condition is as follows:
The fermentation liquid collected is carried out the detection of bacteria containing amount, is diluted to 2 × 10 respectively10About cfu/mL, by two kinds of bacterium solution
In the living bacteria count ratio mixing than 1:0.5, make mixed bacteria liquid.
(4) boiling granulating
Use fluid bed granulator, mixed bacteria liquid is carried out in the ratio of 1:20 (V:W) with the glucose powder that specification is 80 mesh
Pelletize.In pelletization, controlling wind pushing temperature is 60 DEG C, and leaving air temp is 50 DEG C, and air quantity is 3000m3/ h, during every batch pelletize
Between be 30min.According to said method prepare product, use the living bacteria count in plating dilutions counting method detection product, final products
Final concentration >=2 × 10 of bacterial content8cfu/g。
Embodiment two:
(1) activated spawn
Prepare bacillusmusilaginosiengineering NK-BM (CGMCC No.12230), bacillus subtilis NK-BS (commercially available) two kinds micro-
Biological preservation inclined-plane, with inoculating loop picking 1 ring thalline, is seeded in respective Shake flask medium respectively, and whole process needs
Sterile working.By following condition activated strains:
(2) fermentation seed liquid is prepared
Use 100L fermentation system to prepare fermentation seed liquid, prepare the seed culture medium of above two microorganism respectively, add
Entering in 100L fermentation tank, liquid amount is 70%, and sterilizing is cooled to room temperature.Bacterium solution after activation is pressed the inoculum concentration of 2%, respectively
It is seeded in above-mentioned seed culture medium, cultivates to logarithmic (log) phase by respective condition of culture, make fermentation seed liquid.Two kinds of microorganisms
Seed culture medium and fermentation condition as follows:
(3) liquid fermentation
The liquid fermentation using 1t fermentation system to carry out two kinds of microorganisms produces, respectively the fermentation training of two kinds of microorganisms of preparation
Supporting base, add in 1t fermentation tank, liquid amount is 70%, and sterilizing is cooled to room temperature.By the inoculum concentration of 1%, the fermentation that will prepare
Seed liquor is seeded in above-mentioned respective fermentation medium respectively, cultivates to stable phase by respective fermentation condition, collects fermentation liquid.
The fermentation medium of two kinds of microorganisms is identical with seed culture medium, and fermentation condition is as follows:
The fermentation liquid collected is carried out the detection of bacteria containing amount, is diluted to 2 × 10 respectively10Cfu/mL, by two kinds of bacterium solution by having
Effect viable count mixes than the ratio for 1:1, makes mixed bacteria liquid.
(4) boiling granulating
Use fluid bed granulator, mixed bacteria liquid is carried out in the ratio of 1:20 (V:W) with the glucose powder that specification is 80 mesh
Pelletize.In pelletization, controlling wind pushing temperature is 55 DEG C, and leaving air temp is 45 DEG C, and air quantity is 2500m3/ h, during every batch pelletize
Between be 25min.According to said method prepare product, use the living bacteria count in plating dilutions counting method detection product, final products
Final concentration >=2 × 10 of bacterial content8cfu/g。
Embodiment three:
(1) activated spawn
Prepare bacillusmusilaginosiengineering NK-BM (CGMCC No.12230), bacillus subtilis NK-BS (commercially available) two kinds micro-
Biological preservation inclined-plane, with inoculating loop picking 1 ring thalline, is seeded in respective Shake flask medium respectively, and whole process needs
Sterile working.By following condition activated strains:
(2) fermentation seed liquid is prepared
Use 100L fermentation system to prepare fermentation seed liquid, prepare the seed culture medium of above two microorganism respectively, add
Entering in 100L fermentation tank, liquid amount is 70%, and sterilizing is cooled to room temperature.Bacterium solution after activation is pressed the inoculum concentration of 2%, respectively
It is seeded in above-mentioned seed culture medium, cultivates to logarithmic (log) phase by respective condition of culture, make fermentation seed liquid.Two kinds of microorganisms
Seed culture medium and fermentation condition as follows:
(3) liquid fermentation
The liquid fermentation using 1t fermentation system to carry out two kinds of microorganisms produces, respectively the fermentation training of two kinds of microorganisms of preparation
Supporting base, add in 1t fermentation tank, liquid amount is 70%, and sterilizing is cooled to room temperature.By the inoculum concentration of 1%, the fermentation that will prepare
Seed liquor is seeded in above-mentioned respective fermentation medium respectively, cultivates to stable phase by respective fermentation condition, collects fermentation liquid.
The fermentation medium of two kinds of microorganisms is identical with seed culture medium, and fermentation condition is as follows:
The fermentation liquid collected is carried out the detection of bacteria containing amount, is diluted to 2 × 10 respectively10Cfu/mL, by two kinds of bacterium solution by having
The ratio mixing than 1:2 of the effect viable count, makes mixed bacteria liquid.
(4) use fluid bed granulator, mixed bacteria liquid is entered in the ratio of 1:20 (V:W) with the glucose powder that specification is 80 mesh
Row pelletize.In pelletization, controlling wind pushing temperature is 60 DEG C, and leaving air temp is 50 DEG C, and air quantity is 2000m3/ h, every batch pelletize
Time is 20min.According to said method prepare product, use the living bacteria count in plating dilutions counting method detection product, finally produce
Final concentration >=2 × 10 of product bacterial content8cfu/g。
Concrete application: with Rhizoma Solani tuber osi as subjects, the Water Soluble Compound microbial manure that the research present invention prepares
Effect in terms of Rhizoma Solani tuber osi volume increase, quality raising and soil improvement.
For studying thing: Rhizoma Solani tuber osi, kind be gram newly.
Test site: Keshan farm, Keshan County, Qiqihaer City of Heilongjiang Province.
EXPERIMENTAL DESIGN: set 4 process respectively, is repeated 3 times (in embodiment 1～3, each sample is tested respectively), and random district group is arranged
Row, the area of each experimental plot processing 1 is 20m2, the plot area processing 2～4 is 1 mu, and the fertilizer according to the form below is executed
Consumption is tested.
Table 1 experimental plot arranges
| Process | Fertilizer type and consumption (every mu) |
| 1 | CK |
| 2 | Carbamide 20kg, general calcium 20kg, 50% potassium sulfate 10kg |
| 3 | Composite microbiological fertilizer 5kg |
| 4 | Composite microbiological fertilizer 10kg |
Process of the test: process 1 and only use biological organic fertilizer by the consumption of 1000kg/ mu, processes 2,3,4 respectively by table 1
The fertilizer amount of each correspondence mixes with the biological organic fertilizer of 1000kg/ mu, along seed furrow row replacement or is spreaded manuer in holes by mixed fertilizer
On potato seed block, then earthing.
Test period: on May 13rd, 2015 applies fertilizer, surveys and produces on October 6th, 2015.
Testing index: detection is divided into two aspects, economic characters and yield, an economic characters bag being to measure Rhizoma Solani tuber osi
Include that plant height, subterraneous stem length, branch's number, plant division number, stem be thick, individual plant tuber number and individual plant potato heavily etc., yield presses experimental plot area
It is converted into per mu yield.Another is the quality of Rhizoma Solani tuber osi, and in detection potato block, vitamin C, reducing sugar, crude protein and starch contain respectively
Amount, the Potato Quality between relatively more each process, each index is the meansigma methods repeating experiment.
Field management: during test, the prevention and control of plant diseases, pest control, irrigation, weeding between each process group, other field management one such as topdress
Cut and as usual carry out.The growing way situation of routine observation Rhizoma Solani tuber osi during test.
Result of the test:
(1) different disposal is on Rhizoma Solani tuber osi economic characters and the impact of yield
As shown in table 2 below, the survey showed that for the economic characters of Rhizoma Solani tuber osi, uses the process group of composite microbiological fertilizer
(process 3, process 4) relatively general chemistry fertilizer group (processing 2) and matched group (process), its Rhizoma Solani tuber osi has more preferable economy
Shape, and amount of application is the biggest, and the growing way of Rhizoma Solani tuber osi is the best.After using 10kg/ mu composite microbiological fertilizer (processing 4), its plant height,
Stem is thick, individual plant tuber number and individual plant potato the most respectively than general chemistry fertilizer group (processing 2) add 3.3cm, 2.8mm, 1.1 and
173.6g。
In terms of the yield of Rhizoma Solani tuber osi, use general chemistry fertilizer and potato yield all can be made to increase with composite microbiological fertilizer
Add.But in per mu yield, the process group using composite microbiological fertilizer is significantly improved than the process group of general chemistry fertilizer,
The composite microbiological fertilizer process group (processing 4) wherein using 10kg/ mu is the highest, and its per mu yield is 1504kg, relatively general chemistry
Fertilizer group (processing 2) improves 255.9kg, volume increase 20.5%.
Table 2 different disposal is on Rhizoma Solani tuber osi economic characters and the impact of yield
(2) different disposal impact on Potato Quality
Table 3 is the impact on Potato Quality of each different disposal, and result shows to use composite microbial prepared by the present invention
After thing, the quality of Rhizoma Solani tuber osi has substantially rising, is embodied in: compared with general chemistry fertilizer group (processing 2), complex microorganism
The potato ball of fertilizer group (process 3, process 4) is significantly improved, wherein in terms of the content of vitamin C, reducing sugar and starch
Content about Potato Quality most important index starch has been brought up to 16.95% by 13.74%, adds 3.21%.
The impact on Potato Quality of table 3 different disposal
(3) soil improvement
Before determining fertilising respectively and survey the unit weight of antenatal pedotheque, organic matter, full nitrogen, rapid available phosphorus, the containing of available potassium
The soil root system such as amount, before and after investigation fertilising, the situation of change of soil root system is as shown in table 4 below.Result shows, uses composite microbial
After thing fertilizer, the index of soil property and fertility has obtained a certain degree of improvement.It is embodied in:
The soil weight can directly reflect the physical behavior of soil, and before applying fertilizer, the unit weight of experimental plot soil is
0.93g/cm3, matched group and after applying general chemistry fertilizer group, the soil weight is relative to not occurring bigger changing before fertilising
Become, and the soil weight using the process group (process 3, process 4) of the composite microbiological fertilizer of the present invention occurs in that certain increasing
Add so that the soil in this region is more suitable for the growth of crops, show that composite microbiological fertilizer is improving soil physical properties side
Mask has certain effect.
Each process group soil fertility is measured, after result shows to use the composite microbiological fertilizer of the present invention, in soil
Organic matter, rapid available phosphorus, available potassium all have increase in various degree, and reason mainly composite microbiological fertilizer is contained within can be efficient
The microorganism of dissolving phosphor and dissolving potassium, uses the mineral phosphorus in rear decomposable asymmetric choice net soil, potassium becomes rapid available phosphorus and available potassium, it is simple to crop profit
The bio-diversity of soil with, the growth and breeding improving action in soil of the microorganism simultaneously so that the organic matter in soil
Increased.
Table 4 soil improvement survey result
Claims (8)
1. a strain bacillusmusilaginosiengineering NK-BM(Bacillus mucilaginosus), it is preserved on May 4th, 2016
China Committee for Culture Collection of Microorganisms's common micro-organisms center, is called for short CGMCC, and deposit number is CGMCC
No.12230。
2. a Water Soluble Compound microbial manure, it is characterised in that: comprise bacillusmusilaginosiengineering NK-BM(Bacillus
Mucilaginosus) and bacillus subtilis NK-BS(Bacillus subtilis) two kinds of strains, and to use glucose be auxiliary
Expect to prepare through pelletize, described bacillusmusilaginosiengineering NK-BM(Bacillus mucilaginosus) and bacillus subtilis NK-
BS(Bacillus subtilis) living bacteria count ratio be 1:0.5 ~ 2, described bacillusmusilaginosiengineering and bacillus subtilis
The living bacteria count of bacterium is 1 ~ 5 × 108 cfu/g。
3. the preparation method of the Water Soluble Compound microbial manure described in claim 1, it is characterised in that: comprise the steps:
(1) preparation of bacillusmusilaginosiengineering tunning: by bacillusmusilaginosiengineering bacterial strain from inclined plane inoculating to shake-flask culture
Activation culture 10 ~ 12 h in base, cultivates 12 ~ 16 h in being seeded to seed tank by the inoculum concentration of 1.5 ~ 2%, the seed liquor that will obtain
Being seeded to produce in fermentation tank by the inoculum concentration of 1 ~ 1.2%, fermentation condition is rotating speed 150 ~ 200 r/min, dissolved oxygen ＞ 10%, fermentation
PH value is 6.5 ~ 7.5, and ferment 36 ~ 48 h, it is thus achieved that bacillusmusilaginosiengineering tunning, and the fermentation of described bacillusmusilaginosiengineering is produced
The bacterial content of thing is 2 ~ 4 × 1010cfu/mL；
(2) preparation of bacillus subtilis (Ehrenberg) Cohn fermented product: by bacillus subtilis strain in inclined plane inoculating to Shake flask medium
Activation culture 8 ~ 12 h, cultivates 8 ~ 12 h in being seeded to seed tank by the inoculum concentration of 1.5 ~ 2% and obtains seed liquor, by seed liquor by 1
The inoculum concentration of ~ 1.2% is seeded to produce in fermentation tank, and fermentation condition is rotating speed 150 ~ 200 r/min, dissolved oxygen ＞ 8%, and fermentation pH is
6.5~7.5；Fermentation 24 ~ 36 h, obtain bacillus subtilis (Ehrenberg) Cohn fermented product, the bacterial content of described bacillus subtilis (Ehrenberg) Cohn fermented product
It is 3 ~ 5 × 1010cfu/mL；
(3) bacillus subtilis (Ehrenberg) Cohn fermented product and bacillusmusilaginosiengineering tunning are pressed living bacteria count than for 1:0.5 ~ 2
Mixing is compounding, makes mixed bacteria liquid；
(4) using fluid bed granulator that mixed bacteria liquid and dextran particles carry out fluidized granulating, mixed bacteria liquid and glucose press V:W
Mix than for 1:20 ~ 50, make the fertiliser granulates that size is 1.2 ~ 4.5 mm.
4. the preparation method of Water Soluble Compound microbial manure as claimed in claim 3, it is characterised in that: described step (1)
The formula of middle Shake flask medium is: sucrose 10 ~ 15 g/L, (NH4)2SO4 15~20 g/L、MgSO4 5~10 g/L、K2HPO4
20 ~ 25 g/L, yeast extract 2 ~ 5 g/L, FeCl3 0. 05~0. 1 g/L、CaCO31 ~ 5 g/L, starch 20 ~ 30 g/L, training
Supporting base original ph is 7.0 ~ 7.5.
5. the preparation method of Water Soluble Compound microbial manure as claimed in claim 3, it is characterised in that: described step (1)
The formula of the culture medium of middle fermentation tank is: brown sugar 2 ~ 4 g/L, starch 10 ~ 15 g/L, analysis for soybean powder 1 ~ 2 g/L, yeast extract 0.3 ~
0.5 g/L、(NH4)2SO4 2~2.5 g/L、KH2PO4 2~5 g/L、MgSO4 0.5~1 g/L、CaCO30.1 ~ 0.2 g/L and
FeCl30.01 ~ 0.05 g/L, initial pH value of medium is 7.0 ~ 7.5.
6. the preparation method of Water Soluble Compound microbial manure as claimed in claim 3, it is characterised in that: described step (2)
The formula of middle Shake flask medium is: glucose 10 ~ 15 g/L, peptone 8 ~ 10 g/L, NaCl 5 ~ 10 g/L, CaCO3 0.5
~ 1 g/L, the initial pH of culture medium are 7.0 ~ 7.5.
7. the preparation method of Water Soluble Compound microbial manure as claimed in claim 3, it is characterised in that: described step (2)
The culture medium prescription of middle fermentation tank is: brown sugar 10 ~ 15 g/L, starch 2 ~ 2.5 g/L, bean cake 12 ~ 15 g/L, wheat bran 1 ~ 2 g/L,
Yeast extract 1 ~ 2 g/L, KH2PO4 2~3 g/L、K2HPO4 1~2 g/L、CaCO3 0.5~1 g/L、MgSO4 0.5~1 g/L、
CaCl2 0.5~1 g/L、MnSO4 0.2 ~0.5 g/L、FeCl30.01 ~ 0.05 g/L and EDTA 0.01 ~ 0.1 g/L, cultivates
Base original ph is 7.0 ~ 7.5.
8. the preparation method of Water Soluble Compound microbial manure as claimed in claim 3, it is characterised in that: described step (4)
The wind pushing temperature of middle fluid bed granulator is 50 ~ 60 DEG C, and leaving air temp is 40 ~ 50 DEG C, and air quantity is 2000 ~ 3000 m3/ h, during pelletize
Between be 20 ~ 30 min.
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