CN113396937A - Composition of multienzyme and biological bactericide - Google Patents

Composition of multienzyme and biological bactericide Download PDF

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Publication number
CN113396937A
CN113396937A CN202010188385.4A CN202010188385A CN113396937A CN 113396937 A CN113396937 A CN 113396937A CN 202010188385 A CN202010188385 A CN 202010188385A CN 113396937 A CN113396937 A CN 113396937A
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multienzyme
percent
biological
composition
biological bactericide
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Inventor
陈永杰
林枫
包来仓
陈剑钊
郑惠玲
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Mengzhou Yunda High Tech Biotechnology Co ltd
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Mengzhou Yunda High Tech Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a composition of multienzyme and biological bactericide, wherein the active ingredients of the composition are formed by combining multienzyme and biological bactericide, and the weight ratio of the multienzyme to the biological bactericide is 1: 50-30: 1. the fungicide composition can be formulated into granules according to a known method. After the two are compounded, the synergistic effect is obvious, the dosage is reduced, and the generation of drug resistance is delayed.

Description

Composition of multienzyme and biological bactericide
Technical Field
The invention relates to a composition of multienzyme and biological bactericide, which comprises one or more of multienzyme and biological bactericide as effective components, and belongs to the technical field of pesticide.
Background
The multienzyme is formed by high-efficiency active multienzyme composite culture, after the multienzyme composite culture is applied to soil, beneficial bacteria are massively propagated, a protective barrier is formed around the root system of crops, the harmful bacteria are inhibited by utilizing the principle of 'inhibiting bacteria with bacteria', substances secreted by the beneficial bacteria in the propagation process can inhibit and kill the harmful bacteria, meanwhile, ineffective nutrients in the soil are activated, and the nutrient imbalance and nutrient deficiency symptoms disappear. The multienzyme can effectively activate the in vivo anti-stress immune system of the crops and enhance the comprehensive resistance (drought resistance, cold resistance, heat resistance, alkali resistance, waterlogging and diseases) of the crops; inducing crops to generate disease-resistant factors and improving the bactericidal capacity of the crops; protecting crop cell membrane, enhancing cell activity, and improving photosynthetic efficiency; supplementing trace elements required by plant growth; pesticide residue is effectively degraded, and quality is improved; effectively relieve the phytotoxicity and fertilizer damage of the herbicide.
The biological bactericide comprises a botanical bactericide, a biological bactericide and a microbial bactericide, and the active ingredients of the biological bactericide comprise various amino-oligosaccharin, paecilomyces lilacinus, bacillus amyloliquefaciens and the like.
The amino-oligosaccharin is also called agricultural special chitosan, can change the soil microflora, promote the growth of beneficial microorganisms and inhibit some plant pathogenic bacteria. Meanwhile, the chitosan oligosaccharide has a certain direct inhibition effect on various plant pathogenic bacteria, and by influencing the germination of fungal spores, the variation of hypha morphology, the change of intracoronary biochemistry and the like are induced; the chitosan oligosaccharide can also induce disease resistance of plants, excite genes in the plants, generate chitinase, glucanase, phytoalexin, PR protein and the like with disease resistance, and have the effects of immunizing and killing various fungi, bacteria and viruses.
Allicin is a plant source bactericide, is mainly used for preventing and treating bacterial diseases, can change the characteristics of germ cell membranes through lipid oxidation and other actions, and can competitively inhibit or inactivate thiolyases of thalli by combining oxygen atoms in molecules with thiolyases in cysteine molecules necessary for bacterial growth and propagation, thereby achieving the effect of inhibiting bacterial growth and propagation.
The biological bactericide has the characteristics of strong intraroot performance, high quick-acting performance, long persistent period and the like, is mainly used for preventing and treating tobacco virus diseases, tomato late blight, cotton verticillium wilt, rice blast and the like, cabbage soft rot and cucumber bacterial angular leaf spot, and also has good prevention and treatment effects on kiwi canker, citrus canker and the like; can be used for foliar spraying and soil treatment.
Through research, the multi-enzyme and one or more of the biological bactericides are combined, so that the synergistic effect is obvious after the multi-enzyme biological bactericide is compounded, the dosage is reduced, the generation of drug resistance is delayed, and the synergistic effect is realized.
Disclosure of Invention
The invention provides one or a combination of a plurality of enzymes and biological bactericides, which has obvious synergistic effect after compounding, reduces the dosage, delays the generation of drug resistance and has the functions of increasing yield and enhancing the effect.
The invention relates to a multienzyme and biological bactericide, wherein the weight ratio of the multienzyme to the biological bactericide is 1: 50-30: 1, preferably 1:30-20: 1.
the composition can be made into various agriculturally acceptable dosage forms. Such as granules, suspensions, etc., according to methods known to those skilled in the art.
The invention relates to a multienzyme and one or more of biological bactericides, which comprises 1 to 70 percent of active ingredients, 1 to 70 percent of carriers, 1 to 40 percent of binders and 1 to 50 percent of fillers.
The invention relates to a multienzyme and one or more of biological bactericides, wherein a suspending agent contains 1-40% of active ingredients, 1-10% of wetting agents, 1-10% of dispersing agents, 0.1-2% of thickening agents and water which are added to 100%.
The carrier is selected from one or more of alumina, bentonite, zeolite, diatomite, kaolin and high-molecular exchange resin.
The binder is selected from one or more of xanthan gum, sodium alginate, polyvinyl alcohol, sodium polyacrylate, dextrin, CMC, SF-04 and bentonite.
The filler of the invention is kaolin, diatomite, white carbon black, attapulgite and light calcium carbonate.
The wetting agent is a mixture of sodium dodecyl sulfate, sodium alkyl naphthalene sulfonate, fatty alcohol polyglycol ether sulfate, fatty alcohol-polyvinyl ether, nonylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, alkyl naphthalene formaldehyde condensate, alkyl naphthalene sulfonate and an anion wetting agent.
The dispersant is one or more of NNO, sodium lignosulfonate, calcium lignosulfonate, sodium polyacrylate, methylene dinaphthalene sulfonate, alkyl amidotaurine, methylene dinaphthalene sulfonate formaldehyde condensate, polyoxyethylene polyoxypropylene block copolymer, carboxymethyl cellulose, polyvinyl alcohol, polyvinylpyrrolidone, sodium tripolyphosphate and alkyl naphthalene sulfonate polycondensate.
The disintegrating agent is one or more of urea, sodium carboxymethyl cellulose, ammonium sulfate, modified starch, inorganic salt and PEG.
The thickening agent is one or more of xanthan gum, polyvinyl alcohol, sodium alginate, starch and organic bentonite.
The emulsifier is alkylbenzene sulfonate, alkyl naphthalene sulfonate, alkyl diphenyl ether sulfonate, naphthalene sulfonic acid formaldehyde condensate and alkyl succinate sulfonate.
Detailed Description
Example 1: 10% multienzyme-amino-oligosaccharin suspending agent
1% of multienzyme, 9% of amino-oligosaccharin, AEO-32%, 6% of sodium tripolyphosphate, 1.5% of xanthan gum and water which are added to 100% are fully mixed and ground to obtain the 10% of multienzyme-amino-oligosaccharin suspending agent.
Example 2: 20% multienzyme-amino-oligosaccharin suspending agent
2% of multienzyme, 18% of amino-oligosaccharin, AEO-34%, 5% of sodium tripolyphosphate, 3% of xanthan gum and water which are added to 100% are fully mixed and ground to obtain 20% of multienzyme-amino-oligosaccharin suspending agent
Example 3 5% Multi-enzyme allicin suspension
1.5 percent of multienzyme, 4.5 percent of garlicin, T-604 percent of sodium polyacrylate, 1 percent of polyvinyl alcohol and water are fully mixed and ground to obtain the 5 percent multienzyme-garlicin suspending agent after the completion of 100 percent.
Example 4: 10% multienzyme allicin suspending agent
2 percent of multienzyme, 8 percent of garlicin, T-605 percent, 9 percent of sodium polyacrylate, 2 percent of polyvinyl alcohol and water which are added to 100 percent and fully mixed and ground to obtain 10 percent of multienzyme-garlicin suspending agent
Example 5: 1% multienzyme-amino-oligosaccharin granule
0.1% of multienzyme, 0.9% of amino-oligosaccharin, 72% of carrier, 7% of binder and 20% of filler. To obtain 1% of multienzyme-amino-oligosaccharin granules.
Example 6: 5% multienzyme-amino-oligosaccharin granule
4% of multienzyme, 1% of amino-oligosaccharin, 67% of carrier, 8% of binder and 20% of filler. To obtain 5% of multienzyme-amino-oligosaccharin granules.
Example 7: 2% multienzyme allicin granule
0.5 percent of multienzyme, 1.5 percent of garlicin, 70 percent of carrier, 7 percent of adhesive and 21 percent of filler. To obtain the garlicin granules with 2 percent of multienzyme.
Example 8 6% multienzyme allicin granules
6% of multienzyme, 4% of allicin, 68% of carrier, 10% of binder and 16% of filler. To obtain the 6 percent multienzyme allicin granules.
Example 9: indoor toxicity assay
The products of the above examples were subjected to an indoor virulence assay. The test uses a hyphal growth rate method. A sterile aqueous solution containing appropriate amounts of dimethyl sulfoxide and Tween 80 was used as a control. Adding 2ml of the liquid medicine into a conical flask with 38ml of hot culture medium, shaking up, quickly pouring into glass culture dishes with the diameter of 90mm, pouring 13ml of culture medium with the liquid medicine into each culture dish, repeating the three treatments, horizontally standing, cooling and preparing into a flat plate. Each dish was inoculated with a bacterial cake of 0.4cm diameter. All operations were performed aseptically on a clean bench. Culturing in a constant temperature sterile incubator at about 26 deg.C for 3d, and taking out. Measuring the colony diameter of each treatment by cross method, and calculating average value of colony diameter, average value of hypha growth inhibition and EC50Then, the Sun Yunpei method is used to calculate the co-toxicity coefficient (CTC).
The co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the cotoxicity coefficient (CTC) is less than or equal to 80, and antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect.
Actually measured virulence index (ATI) = (standard agent EC)50Reagent for test EC50）×100
Theoretical virulence index (TTI) = (virulence index of A x percent of A in the mixture) + (virulence index of B x percent of B in the mixture)
Cotoxicity coefficient (CTC) = [ actually measured virulence coefficient (ATI)/theoretical virulence coefficient of mixture (TTI) ] × 100
Co-toxicity coefficient (CTC).
TABLE 1 indoor virulence results for the products of the examples against tomato downy mildew
Figure 512478DEST_PATH_IMAGE002
As can be seen from the above table, the TCT values of the multienzyme and one or more of the biological bactericides exceed 120 within the proportion range of 1:30-20:1, which indicates that the synergistic effect is good.
Example 14: plot efficacy test
The agents prepared in examples 1 to 12 of the present invention and individual agents of each component were used as test pesticides to carry out tests, and the test schemes and the control results are shown in table 2.
TABLE 2 treatment protocol and results of the drug efficacy test
Figure 1
As can be seen from the table above, the combination of one or more of the multienzyme and the biological bactericide widens the bactericidal spectrum, reduces the dosage and has good bactericidal effect.
Example 15: treatment of production increase data
Selecting plots with the same area and the same water and fertilizer conditions for field test, treating the plots with clear water in comparison, measuring the yield of crops after the picking period of the test is finished, and calculating the yield increasing effect, wherein the test results are shown in Table 3.
TABLE 3 test yield increase effect
Figure 147039DEST_PATH_IMAGE006
As can be seen from the above table, the combination of one or more of the multienzyme and the biological bactericide not only has good bactericidal effect, but also has obvious yield increasing effect. Can be applied to the field of agricultural production.
The above description of the embodiments is only intended to facilitate the understanding of the method of the invention and its core idea. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.

Claims (4)

1. A composition of multienzyme and biological bactericide is characterized in that: the active component of the microbial agent is composed of multienzyme and one or more of biological bactericides.
2. The multi-enzyme and biological biocide composition as claimed in claim 1, wherein: the weight ratio of the multienzyme to the biological bactericide is 1: 50-30: 1.
3. the multi-enzyme and biological biocide composition as claimed in claim 1, wherein: the weight ratio of the multienzyme to the biological bactericide is 1:30-20: 1.
4. the multi-enzyme and bio-biocide composition according to claim 1 and claim 2, wherein: the composition can be made into granule and suspension.
CN202010188385.4A 2020-03-17 2020-03-17 Composition of multienzyme and biological bactericide Pending CN113396937A (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009739A1 (en) * 1989-03-01 1990-09-07 The Public Health Research Institute Of The City Of New York, Inc. Novel bacteriocin compositions for use as enhanced broad range bactericides and methods of preventing and treating microbial infection
CN102964178A (en) * 2012-11-13 2013-03-13 湖南泰谷生物科技股份有限公司 Composite microbial bactericide, and preparation method and application thereof
CN104430390A (en) * 2014-10-24 2015-03-25 青岛瀚生生物科技股份有限公司 Amino-oligosaccharin and ethaboxam compounded agricultural fungicide
CN105145632A (en) * 2015-07-20 2015-12-16 潘灿平 Composite bacterial enzyme system with multiple effects and application thereof
CN105981743A (en) * 2015-01-29 2016-10-05 陕西美邦农药有限公司 Antimicrobial yield-increase composition containing hypersensitive protein
CN110074139A (en) * 2019-05-13 2019-08-02 河南地卫士生物科技有限公司 A kind of biological pesticide that Trichoderma compounds with super quick albumen complex enzyme
CA3101860A1 (en) * 2018-06-05 2019-12-12 Novozymes Bioag A/S Methods of protecting a plant from a fungal pest

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009739A1 (en) * 1989-03-01 1990-09-07 The Public Health Research Institute Of The City Of New York, Inc. Novel bacteriocin compositions for use as enhanced broad range bactericides and methods of preventing and treating microbial infection
CN102964178A (en) * 2012-11-13 2013-03-13 湖南泰谷生物科技股份有限公司 Composite microbial bactericide, and preparation method and application thereof
CN104430390A (en) * 2014-10-24 2015-03-25 青岛瀚生生物科技股份有限公司 Amino-oligosaccharin and ethaboxam compounded agricultural fungicide
CN105981743A (en) * 2015-01-29 2016-10-05 陕西美邦农药有限公司 Antimicrobial yield-increase composition containing hypersensitive protein
CN105145632A (en) * 2015-07-20 2015-12-16 潘灿平 Composite bacterial enzyme system with multiple effects and application thereof
CA3101860A1 (en) * 2018-06-05 2019-12-12 Novozymes Bioag A/S Methods of protecting a plant from a fungal pest
CN110074139A (en) * 2019-05-13 2019-08-02 河南地卫士生物科技有限公司 A kind of biological pesticide that Trichoderma compounds with super quick albumen complex enzyme

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Application publication date: 20210917