KR101280679B1 - Agent for controlling diseases occurring in the stage of raising rice seedlings - Google Patents

Abstract

An object of this invention is to provide the microbial pesticide which is excellent in a control effect, and which has a low environmental impact, against the many disease which arises at the time of rice seedling growth. According to the present invention, there is provided a control agent of a disease occurring at the time of rice seedling, containing a filamentous fungus belonging to the genus Talaromyces having an antagonistic action against a pathogen causing the disease at the time of rice seedling growth.

Description

AGENT FOR CONTROLLING DISEASES OCCURRING IN THE STAGE OF RAISING RICE SEEDLINGS}

The present invention relates to a control against diseases occurring at the time of rice seedling growth, and to a method for controlling diseases occurring at the time of rice seedling growth using the same.

Cultivation of healthy and homogeneous hair is one of the most important tasks in the cultivation of rice. The germination, from the seedling stage, to the stage of nursery are susceptible to various diseases, and thorough control is usually carried out by chemical agents.

Conventionally, as a chemical synthetic agent used for controlling diseases during rice seedling growth, DMI agents such as tripumizol are generally used for seed pathogenic diseases such as filamentous seed infectious diseases caused by filamentous fungi, heat diseases, and sesame pattern disease. Oxylic acid and the like are widely used for bacterial rice bran, moth, and streaks, which are seed infectious diseases caused by bacteria. Moreover, about rice bran disease which is a soil infectious disease, control is carried out by the agent containing hydroxyisoxazole, benomil, etc.

However, pathogens with low sensitivity or resistance to the chemical synthetic drugs have emerged and become a problem. In addition, there is a need to reduce the amount of chemical synthetic drugs used and the number of times of use in growing crops, due to concerns about the safety of food or the impact on the environment. In addition, there is a demand for the development of a technology / commodity capable of reducing the amount of use and the number of times of use of the chemical synthetic drugs, while increasing the demand for food safety and the like. As such a technique, specifically, there exists a chemical product with high stability which can be used for a natural product, a food additive, etc., an agricultural chemical using a living organism, etc.

Background Art Control of microorganisms is known as a disease control technique using living organisms. For example, Patent Literature 1 describes a microbial preparation for rice disease control including Fusarium bacteria, and Patent Literature 2 describes a microbial preparation for rice seed disease control containing Pseudomonas bacteria.

In addition, a microbial fungicide BioTrust (Idemitsu Koyama Co., Ltd.) containing talaromyces flavus, a type of filamentous fungus, as an active ingredient is used as a control agent for stem leaf diseases for strawberry anthracnose and strawberry powdery mildew. However, it has not been reported so far that Thalaromyces bacteria have an effect on disease control in other crops.

Patent Document 1: Japanese Patent Application Laid-Open No. 11-89562

Patent Document 2: Japanese Patent Laid-Open No. 2002-17343

DISCLOSURE OF INVENTION

Problems to be solved by the invention

The present invention has been made in view of the above, and an object of the present invention is to provide a microbial pesticide that is safe and has low environmental impacts against many diseases occurring during rice seedling growth.

Means for solving the problem

As a result of intensive studies to solve the above problems, the present inventors have found that filamentous fungi belonging to the genus Talaromyces have a control effect against many diseases occurring during rice seedling growth. The invention has been completed.

That is, the gist of the present invention is as follows.

(1) A pest control agent that occurs during the seedling stage of rice containing filamentous fungi belonging to the genus Talaromyces, which has an antagonistic action against pathogens causing disease during seedling stage of rice.

(2) Control agent of disease that pathogen causing disease at seedling time of rice occurs at seedling time of rice according to (1) which is rice seed infectious pathogen or soil infectious pathogen.

(3) pathogens that cause disease at the time of seedling growth of rice (Gibberella fujikuroi); Pyricularia oryzae; Rice seed germ (Cochliobolus miyabeanus); Bacterial rice almond germ (Pseudomonas glumae); Bacterial rice seed germ (Pseudomonas plantarii); Bacterial rice streaks (Pseudomonas avenae); Occurs during the seedling period of rice according to (2), which is at least one of the genus Fusarium, Pythium, Rhizopus, and Trichoderma Control of pests to do.

(4) A disease control agent that occurs during the seedling period of rice according to any one of (1) to (3), wherein the filamentous fungus belonging to the genus Talaromyces is Talaromyces flavus.

(5) A pest control agent that occurs at the seedling time of rice according to (4), wherein the filamentous fungi belonging to the genus Thalaromyces are Thalaromyces flavus Y-9401 strain (FERM BP-10642).

(6) A step of treating rice seed, seedling, seedling soil or seedling medium with the pest control agent according to any one of (1) to (5). Control of the disease.

Hereinafter, the present invention will be described in detail.

(Disease control agent of the present invention)

The disease control agent of this invention is a disease control agent which arises at the time of rice seedling growth. Here, "mo" means a young plant up to the rice plant. In addition, "seedling" means growing the hair, and "seedling time" means the cultivation time before planting the hair. Seedling seedlings are the number of leaves the seedlings have, such as the first, second and third leaf stages, and may indicate the growth period of the seedlings. In terms of the mock growth period, the peony at the seedling stage, for example, is gathered up to about four leaf stages including incomplete leaves, which are suitable for transplantation in seedling transplantation.

The disease control agent of the present invention contains filamentous fungi belonging to the genus Talaromyces as an active ingredient.

(1) filamentous fungi belonging to the genus Thalaromyces

The filamentous fungi belonging to the genus Thalaromyces used in the present invention are not particularly limited as long as they have an antagonistic action against pathogens causing disease during rice seedling period. Here, "antagonistic action" means the action of reducing the number of cells of the pathogen causing the disease during the seedling period of the target rice, or inhibit the proliferation, that is, antibacterial action. As a bacterium which has an antagonistic action against the pathogen causing a disease at the time of rice seedling growth, the bacterium belonging to the genus Talaromyces flaus species is mentioned, and among them, Talamices flavus Y-9401 strain Preferred is mentioned. Mutant strains of Y-9401 strains can also be used as long as they have the antagonism. The disease control agent of this invention can be used individually by 1 type of the above-mentioned microorganisms, or in combination of 2 or more types so long as it does not affect a disease control effect.

Talaromiques Flabus Y-9401 is a patent microbial deposit center of the Institute of Biotechnology and Industrial Technology, Institute of Commerce and Industry, September 2, 1996 (IPOD), Industrial Technology Research Institute, Ibaraki Prefecture, Tsukuba, Ibaraki Prefecture. Has been deposited as FERM P-15816 in Higashi-shi 1-1-1 Chuo 6). Then, it was transferred to the international deposit on July 18, 2006 and deposited as FERM BP-10642.

Antagonistic action against pathogens causing disease during seedling period of rice, for example, placed on the same plate side-by-side with pathogens causing disease during seedling period of rice, and incubated for 3 to 14 days at 15-35 ℃ Then, it can confirm by observing the situation of the growth suppression of the pathogen which causes a disease at the seedling time of rice by the test bacteria.

The filamentous fungi belonging to the genus Talaromyces used in the present invention can be cultured in the same manner as in the conventional filamentous fungi. For example, filamentous fungi belonging to the genus Talaromyces can be grown by a liquid culture method such as a reciprocating liquid culture cultured in a liquid medium, a culture of a jar fermenter, or a solid culture method cultured in a solid medium. In order to produce a good yield of spores of filamentous fungi belonging to the genus Talaromyces, a solid culture method is more suitably used. About the culture conditions of the filamentous fungi belonging to the genus Talaromyces, it is preferable to carry out under aerobic conditions by methods, such as aeration, stirring, and shaking, and 20-40 degreeC of culture temperature is preferable. The culture period is preferably 3 to 60 days, particularly preferably 3 to 20 days. In addition, it is preferable that the microbial cells of the filamentous fungi belonging to the genus Thalaromyces used in the present invention are spores from the viewpoint of preservation as a disease control product. Therefore, in order to spore the filamentous fungi belonging to the genus Talaromyces, culture conditions such as the composition of the medium, the pH of the medium, the culture temperature, the culture humidity, the oxygen concentration at the time of cultivation, and the like as the spore-forming conditions, are necessary. It is preferable to prepare so that it may suit.

As a liquid medium component used for culturing filamentous fungi belonging to the genus Thalaromyces, a pottodextrose medium or an agar medium is used. In the case of solid culture, a sugar or nitrogen source is contained as necessary, for example, a solid carrier such as a solid component derived from grains such as rice, barley, corn, soybean, wheat bran, soybean residue, or clay mineral containing nutrients. Medium can be used.

Cultures containing filamentous fungi belonging to the genus Talaromyces may be used as they are, but may be used by crushing or slicing the cultures as needed, and may also be used to recover spores mainly from sieves or the like from these cultures. In addition, the cells may be separated from the culture by a liquid such as water or oil, and those obtained as they are or may be concentrated.

The disease control agent of the present invention usually contains 1 × 10 ^{6 to} 1 × 10 ¹² cfu / g, preferably 1 × 10 ^{7 to} 1 × 10 ¹¹ cfu / g, with filamentous fungi belonging to the genus Talaromyces as colony forming units. The culture or the pulverized product of the culture is combined with various kinds of preparations suitable for the use or the method of use.

It is preferable that it is 0.1-99.9 mass%, and, as for the disease control agent of this invention, the content of the culture containing the filamentous fungus belonging to the genus Talalomyces, or the grinding | pulverization of a culture is more preferably 1.0-50.0 mass%.

(2) optional ingredients

In addition to the filamentous fungi belonging to the genus Talaromyces, the disease control agent of the present invention can be used as an optional ingredient as long as it does not interfere with the effects of the present invention. Such optional components may be contained as necessary for the purpose of formulation, stabilization of quality, and the like.

As an arbitrary component used for the disease control agent of this invention, it can be used, for example in combination of 1 type, or 2 or more types of the following components.

For example, solid carriers as extenders include kaolin clay, pyroferrite clay, bentonite, montmorillonite, diatomaceous earth, synthetic hydrous silicon oxide, acid clay, talc, clay, ceramics, quartz, sericite, vermiculite, pearlite, pylori Fine mineral powders such as tuff, anthracite, limestone, coal stone and zeolite; Inorganic compounds such as salt, carbonate, sulfate, nitrate and urea; Rice husks, bran, crab shells, shrimp shells, krill shrimp powder, crushed rice, flour, corn ears, peanut shells, bone meal, fishmeal, gourd powder, wood flour, charcoal, hultan, bark flakes, rice husk charcoal, charcoal charcoal, peat moss, atapalzai Fine powders of organic substances such as salts, dried livestock powder, activated carbon, oil foil, starch and hydrolyzate thereof; Soluble extenders, such as D-sorbitol, lactose, maltose, glucosamine, and oligosaccharides, etc. can be used. As the liquid carrier, water, vegetable oil, animal oil, mineral oil, synthetic water-soluble polymer and the like can be used.

Furthermore, if necessary, natural polysaccharides such as casein, gelatin, gum arabic, alginic acid, cellulose, carboxymethyl cellulose, xanthan gum, chitin and chitosan; Polyvinyl alcohols; Polyacrylic acids; Bentonite etc. can be contained as needed for the purpose of thickening, fixation, dispersion | distribution, etc.

Moreover, dihydric alcohols, such as ethylene glycol and propylene glycol, can be contained as needed for the purpose of freezing prevention, etc.

Surfactants, such as anion type, cation type, and amphoteric type, can be contained as needed for the purpose of dispersion stability, aggregation prevention, emulsification, etc.

(Method for producing a disease control agent of the present invention)

A disease control agent containing filamentous fungi belonging to the genus Talaromyces of the present invention can be formulated in a form that is actually easy to use. That is, according to the manufacturing method of a conventional formulation, it can be formulated with a hydrating agent, powder, granule, emulsion, a flowable agent, a coating agent, etc. with various arbitrary components as needed.

For example, a hydrating agent and a powder can be manufactured by mixing or pulverizing mixing the above-mentioned surfactant and the component which stabilizes quality as needed with the solid carrier as mentioned above.

For example, the granules can be prepared by mixing or pulverizing and further mixing the above-described surfactants and components which stabilize the quality to the solid carrier as described above as necessary.

For example, an emulsion may be prepared by emulsifying, dispersing, and the like as described above in liquid carriers such as vegetable oils, animal oils, mineral oils, and the like, or by mixing or pulverizing a component that stabilizes the quality. .

For example, the floorable agent may be used for the purpose of thickening the auxiliary agent as described above in water, for the purpose of freezing prevention of the dihydric alcohol as described above, for the purpose of dispersing the surfactant as described above, And it can manufacture by mixing or grinding-mixing the component which stabilizes quality.

For example, a coating agent can be manufactured by adding an adjuvant to liquid carriers, such as water and oil, mixing, and making it a sol form or a gel form.

(Application method of the disease control agent of this invention)

The pest control agent of the present invention works efficiently to control the pests generated during rice seedling growth by filamentous fungi, bacteria, microbial mediated viruses and the like. In addition, the pest control agent of the present invention acts effectively to control diseases caused by, for example, rice seed infectious germs or soil infectious germs. More specifically, the disease control agent of the present invention, for example, Gibberella fujikuroi, Pyricularia oryzae, Cochliobolus miyabeanus, Bacterial rice almond germ (Pseudomonas glumae), bacterial Pseudomonas plantarii, Bacterial Pseudomonas avenae, Pseudomonas avenae, Fusarium genus, Pythium genus, Rhizopus genus, Trichoderma It is particularly effective in controlling diseases caused by genus bacteria. The disease control agent of the present invention acts on one or two or more of the above pathogens. Among the pathogens that cause disease during the seedling period of rice, not only the rice seedling period but also pathogens causing the disease during the period other than the seedling period, "pathogen causing the disease during the seedling period of rice" is It means the pathogen causing a disease at least at the seedling time of rice, and includes all of the pathogens which cause a disease even at the time other than the seedling time, and the pathogen which does not cause a disease at the time other than the seedling time.

A pest control agent containing filamentous fungi belonging to the genus Talaromyces of the present invention is applied to rice seeds, seedlings, seedling soil or seedling medium of rice for the purpose of controlling various diseases as described above, and the method is used in the form of a formulation or the like. I am chosen according to the disease. As such methods, for example, seed soaking treatment, seed powder treatment, seed coating treatment, seed spreading (including spraying) treatment, soil spreading (including spraying) treatment, soil mixing application, soil irrigation application, seedling box irrigation Application, main application, ground liquid spray, ground solid spray, etc. are mentioned.

In addition, in the application of pest control agents to rice, mixed application or alteration without simultaneous mixing or disinfecting fungicides, insecticides, nematicides, acaricides, herbicides, plant growth regulators, fertilizers, soil improvement materials, etc. It is also possible to apply.

The dosage of a pest control agent containing filamentous fungi belonging to the genus Thalaromyces of the present invention cannot be defined collectively according to the type of the disease, but, for example, in the case of seed immersion treatment, the formulation is 10 to 1000 times ( Mass), and the cell concentration is usually 1 × 10 ^{3 to} 1 × 10 ¹⁰ cfu, preferably 1 × 10 ^{4 to} 1 × 10 ⁹ cfu per 1 ml of immersion liquid.

In the case of treatment of the seed powder, it is preferable to apply the formulation in an amount of 1 to 20% by mass based on the seed mass, and the cell concentration is usually 1 × 10 ^{3 to} 1 × 10 ¹⁰ cfu, preferably 1 × per 1 g of seed mass. 10 ^4-1 x 10 ⁹ cfu.

Seedling box in the case of trial soil spray, is used as for a normal chimyo transplantation (e. G., Area 1800cm ² 50-1000 ml is preferably applied if the sugar spreading is liquid, and the cell concentration is usually 1 × 10 ^{3 to} 1 × 10 ¹⁰ cfu, preferably 1 × 10 ^{4 to} 1 × 10 ¹⁰ cfu per 1 ml of the spraying liquid. .

In the case of soil mixing application, it is preferable to apply 0.1-100 g per seedling box (for example, about 1800 cm ² area) normally used for transplantation of seedlings, and the cell concentration is 1 × 10 ² -1 × 10 ⁹ per 1 ml of soil. cfu, preferably 1 × 10 ^{3 to} 1 × 10 ⁸ cfu.

Seedling box drench in the case of trial, normal seedling box (e. G., Area 1800cm used as a transplant for chimyo ² 50-1000 ml is preferably applied if the sugar spreading is liquid, and the cell concentration is usually 1 × 10 ^{3 to} 1 × 10 ⁹ cfu, preferably 1 × 10 ^{4 to} 1 × 10 ⁸ cfu per 1 ml of the spreading liquid. .

There is no particular limitation on the frequency of application of the pest control agent of the present invention, but the pathogen causing the disease at the time of rice seedling growth is used at the seedling time of rice, for example, at a frequency of 1 to 5 times. Can be suppressed.

Before and after using the pest control agent of the present invention, rice seedlings and rice can be grown by conventional methods.

Although an Example is given to the following and this invention is demonstrated in more detail, it cannot be overemphasized that this invention is not limited only to these Examples.

≪ Preparation Example 1 &

(Production of spores)

A bran was used for the medium, and seedlings of Talamiques Flavus Y-9401 (FERM BP-10642) strain were planted therein and solid-cultured at 30 ° C for 10 days. After the completion of the culture, the culture was dried, and the dried culture was sieved to remove residual bran, thereby obtaining Talaromyces flabus Y-9401 spore-containing powder (4 × 10 ⁹ cfu / g).

<Example 1>

(Production of formulation)

Using the Talaromyces Flabus Y-9401 spore-containing powder obtained in the Production Example 1, 10% by mass of this spore-containing powder, 5% by mass of S0RP0L 5082 (manufactured by Toho Kagaku Kogyo Co., Ltd.) as a surfactant, and clay mineral ( K clay: The raw materials were mixed so that it may become the ratio of 45 mass% of Shokozan Kogyo Co., Ltd., and 40 mass% of glucosamine (made by Kaizu Co., Ltd., Yaizu Corporation), and it mixed and grind | pulverized using the mill grinder, and the formulation of Formulation Example 1 ( 4 × 10 ⁸ cfu / g).

&Lt; Example 1 >

(Rice long leg control examination)

(1) Preparation of Infected Seeds

Rice seeds were harvested from the capital, which is abundant with Kidari disease in the field. This seed was referred to as rice streptococcus bacterial seed.

(2) pharmaceutical treatment

The formulation prepared according to Formulation Example 1 was diluted 200 times (mass) with water, and 5 g of rice streptococcus bacterial seed was added to 15 ml of this solution, and immersed for 48 hours in an artificial gas phase at 30 ° C. in a dark place. Pharmaceutical treatment seeds were used. In addition, a seed infection pathogen was immersed in water in the same manner to prepare a subject (untreated seed).

(3) Upbringing of test plants

After the immersion treatment, seeds of pathogenic bacteria were seeded in a 100 cm ² plastic pot filled with a seedling medium. The area | region which sowed the chemical | medical treatment seed was made into the chemical | medical treatment process group, and the area | region in which the untreated seed was sown. Both treatment and no treatment were repeated three times. After the soil was covered with the seedling raising medium, it was cultivated and managed in a glass greenhouse according to the conventional method.

(4) control effect investigation

Two weeks after sowing, the seedlings with the extreme coating symptoms caused by rice stinging disease were regarded as the seedlings, and onset based on the following equations (1) and (2) using the average value of three repeated seedlings in each treatment group. Seedling rate was calculated. And the control value of the chemical | medical agent treatment tool was computed as a control effect from the calculated incidence ratio based on following formula (2).

Incidence rate (%) = number of onset seedlings / total survey seedlings * 100

Control price = [(non-treatment onset seedling rate-pharmaceutical treatment onset seedling rate) / non-treatment onset seedling rate] * 100

(5) Results

The investigation results are shown in Table 1. As is apparent from Table 1, the disease control agent of the present invention showed a significantly high control effect against the rice disease of rice.

<Example 2>

(Rice blast control test)

(1) Preparation of Infected Seeds

Rice (cultiation: Akita-komachi) seeds were harvested from the capital which is bundling in the field. This seed was referred to as a germ infected seed.

(2) pharmaceutical treatment

The formulation prepared according to Formulation Example 1 was diluted 200 times (mass) with water, and 4 g of the seed germ infection infection was added to 15 ml of this solution, and immersed in an artificial gas phase at 30 ° C. for 48 hours in a dark place to give a drug. Treated seeds were used. The seed of bacterial infection was immersed in water in the same manner to prepare a subject (untreated seed).

(3) Upbringing of test plants

After the immersion treatment, the seed germ infection was sown in a 100 cm ² plastic pot filled with a seedling medium. The area | region which sowed the chemical | medical treatment seed was made into the chemical | medical treatment process group, and the area | region in which the untreated seed was sown. Both treatment and no treatment were repeated three times. After the soil was covered with the seedling raising medium, it was cultivated and managed in a glass greenhouse according to the conventional method.

(4) control effect investigation

Two weeks after the seeding, the seedlings with the plaque lesions expressed on the leaf surface were used as seedlings, and the seedling rate was calculated based on Equation 1 using the average value of the number of seedlings in three iterations of each treatment. And the control value of the chemical | medical agent treatment tool was computed as a control effect based on the said Formula 2 from the calculated onset rate.

(5) Results

The investigation results are shown in Table 2. As is apparent from Table 2, the disease control agent of the present invention showed a remarkably high control effect against rice blast.

<Example 3>

(Bacterial rice blight control test)

(1) Preparation of Infected Seeds

Rice (variety: Husaotome) seed was put into the solution containing bacterial almond germ, and it was forcibly inoculated in the seed under reduced pressure conditions. After air drying, this rice seed was made into bacterial Al blight infection seed.

(2) pharmaceutical treatment

The formulation prepared according to Formulation Example 1 was diluted 200 times (mass) with water, and 5 g of bacterial Alum bacteria infection seed was added to 15 ml of this solution, and immersed for 48 hours in an artificial gas phase at 30 ° C. in a dark place. The seed was treated as drug treatment. Bacterial Al-Merbs infection seeds were immersed in water in the same manner to prepare untreated seeds.

(3) Upbringing of test plants

After the immersion treatment, the bacterial algal pathogen-infected seeds were seeded in a 100 cm ² plastic pot filled with a seedling medium. The area | region which sowed the chemical | medical treatment seed was made into the chemical | medical treatment process group, and the area | region in which the untreated seed was sown. Both treatment and no treatment were repeated three times. After the soil was covered with the seedling raising medium, it was cultivated and managed in a glass greenhouse according to the conventional method.

(4) control effect investigation

After 2 weeks of sowing, seedlings with bacterial alveolar disease such as mock decay, forgery, and bleaching were regarded as the seedlings, and Equation 1 above was expressed using the average value of the number of seedlings in three repetitions of each treatment. The incidence rate was calculated based on this. And the control value of the chemical | medical agent treatment tool was computed as a control effect based on the said Formula 2 from the calculated onset rate.

(5) Results

The investigation results are shown in Table 3. As is apparent from Table 3, the disease control agent of the present invention showed a markedly high control effect against bacterial blight.

<Example 4>

(Bacterial rice blight control test)

(1) Preparation of Infected Seeds

Rice (variety: Hosaotome) seed was thrown in the solution containing bacterial seed germ germ, and it was inoculated in seed forcibly under reduced pressure conditions. After air drying, this rice seed was used as bacterial seed germ infection seed.

(2) pharmaceutical treatment

The formulation prepared according to Formulation Example 1 was diluted 200 times (mass) in water, and 5 g of bacterial seed germ infection seed which had absorbed water at 15 ° C. for 5 days in 15 ml of this solution was charged, and 30 ° C. in a dark place. It was immersed for 24 hours in the artificial vapor phase of to make a pharmaceutical treatment seed. Bacterial seed germ infection seeds were immersed in water in the same manner to prepare a subject (untreated seed).

(3) Upbringing of test plants

After the immersion treatment, bacterial seed germ-infected seeds were seeded in a 100 cm ² plastic pot filled with a seedling medium. The area | region which sowed the chemical | medical treatment seed was made into the chemical | medical treatment process group, and the area | region in which the untreated seed was sown. Both treatment and no treatment were repeated three times. After the soil was covered with the seedling raising medium, it was cultivated and managed in a glass greenhouse according to the conventional method.

(4) control effect investigation

Two weeks after sowing, the seedlings exhibiting the symptoms of bacterial blight such as rot, forgery, and whitening are regarded as the seedlings, and the onset is based on Equation 1 using the average number of seedlings in three repetitions of each treatment. Seedling rate was calculated. And the control value of the chemical | medical agent treatment tool was computed as a control effect based on the said Formula 2 from the calculated onset rate.

(5) Results

The investigation results are shown in Table 4. As is apparent from Table 4, the disease control agent of the present invention showed a markedly high control effect against bacterial blight of rice.

<Example 5>

(Rice stripe control test)

(1) Preparation of Infected Seeds

Rice (variety: Fusaotome) seed was thrown in the solution containing striped germs, and it was inoculated forcibly in the seed under reduced pressure conditions. After air drying, this rice seed was used as a seed germ infection.

(2) pharmaceutical treatment

Formulation Example 1 prepared as described above was diluted 200 times (mass) in water, and 5 g of the streptococcus infection seed which absorbed water at 15 ° C. for 5 days was added to 15 ml of this solution, and an artificial machine at 30 ° C. was placed in a dark place. It was immersed in the inside for 24 hours, and it was made into the chemical | medical treatment seed. Streptococcus infected seeds were immersed in water in the same manner to prepare a subject (untreated seeds).

(3) Upbringing of test plants

After the immersion treatment, streaked germ-infected seeds were seeded in a 100 cm ² plastic pot filled with a seedling medium. The area | region which sowed the chemical | medical treatment seed was made into the chemical | medical treatment process group, and the area | region in which the untreated seed was sown. Both treatment and no treatment were repeated three times. After the soil was covered with the seedling raising medium, it was cultivated and managed in a glass greenhouse according to the conventional method.

(4) control effect investigation

Two weeks after sowing, the seedlings with streaks such as rot, forgery, and browning were expressed as diseased seedlings, and the incidence rate based on Equation 1 using the average value of the number of seedlings in three repetitions of each treatment group. Was calculated. And the control value of the chemical | medical agent treatment tool was computed as a control effect based on the said Formula 2 from the calculated onset rate.

(5) Results

The investigation results are shown in Table 5. As is apparent from Table 5, the disease control agent of the present invention showed a remarkably high control effect against the striped disease of rice.

<Example 6>

(Rice seedling control test by Fusarium bacteria)

(1) Inoculation of pathogen

Fusarium cultures cultured in wheat bran and aquaculture seedling medium were uniformly mixed with the rice seedling medium to obtain soils contaminated with rice worms caused by Fusarium bacteria.

(2) pharmaceutical treatment

Rice (variety: Koshihikari) was sown in a 100 cm ² plastic pot filled with the blight-contaminated soil caused by the Fusarium bacteria. And the solution prepared by diluting the formulation prepared in Formulation Example 1 with water 1000 times (mass) was uniformly sprayed (25 ml) on the soil surface to obtain a chemical treatment tool. Moreover, the pot which made the said chemical | medical agent solution the water was produced, and it was made into the object (untreated tool). Both treatment and untreated groups were repeated twice.

(3) Upbringing of test plants

The pots subjected to the treatment were covered with a seedling medium, and then cultivated and managed in a glass greenhouse according to the conventional method.

(4) control effect investigation

Two weeks after sowing, the seedlings with the appearance of hair follicle disease caused by Fusarium bacteria such as mock decay, forgery, and browning are regarded as the seedlings, and the above equation 1 is obtained using the average value of the number of seedlings in two repetitions of each treatment. The incidence rate was calculated based on this. And the control value of the chemical | medical agent treatment tool was computed as a control effect based on the said Formula 2 from the calculated onset rate.

(5) Results

The investigation results are shown in Table 6. As is apparent from Table 6, the disease control agent of the present invention showed a significantly high control effect against rice bran disease caused by Fusarium bacteria.

&Lt; Example 7 >

(Paddy rice bran control control test with lypus bacteria)

(1) Inoculation of pathogen

Rizopus bacteria cultured in a bran and a rice seedling culture medium were uniformly mixed with the rice seedling growth medium, and the soil was contaminated with rice blight caused by Rizopus bacteria.

(2) pharmaceutical treatment

Rice (variety: Koshihikari) was sown in a 100 cm ² plastic pot filled with soil blight-contaminated soil by Rizopus bacteria. And the solution which diluted Formulation Example 1 by 1000 times (mass) with water was spray-treated (25 ml) uniformly on the said soil surface, and it was set as the chemical | medical agent treatment tool. Moreover, the pot which made the said chemical | medical agent solution the water was produced, and it was made into the object (untreated tool). Both treatment and untreated groups were repeated twice.

(3) Upbringing of test plants

The pots subjected to the treatment were covered with a seedling medium, and then cultivated and managed in a glass greenhouse according to the conventional method.

(4) control effect investigation

Two weeks after sowing, seedlings exhibiting symptoms of hair dryness caused by Rizopus bacteria, such as rot, forgery, and inhibition of root growth, are regarded as seedlings, and the average value of the number of onset seedlings in two repetitions of each treatment group is used. The incidence rate was calculated based on Equation 1. And the control value of the chemical | medical agent treatment tool was computed as a control effect based on the said Formula 2 from the calculated onset rate.

(5) Results

The investigation results are shown in Table 7. As is apparent from Table 7, the disease control agent of the present invention showed a markedly high control effect against rice blight caused by Rizopus bacteria.

&Lt; Example 8 >

 (Paddy rice bran control test by Tricoder bacteria)

(1) Inoculation of pathogen

Trichodermium cultures cultured in bran and aquaculture seedling medium were uniformly mixed with the rice seedling medium to prepare soils contaminated with rice bran by Trichoderma bacteria.

(2) pharmaceutical treatment

Rice (variety: Koshihikari) was sown in a 100 cm ² plastic pot filled with the soil blight contaminated soil by the Trichoderma bacterium. And the solution which diluted Formulation Example 1 to 1000 times (mass) in water was spray-processed (25 ml) uniformly on the soil surface, and it was set as the chemical | medical agent treatment tool. Moreover, the pot which made the said chemical | medical agent solution the water was produced, and it was made into the object (untreated tool). Both treatment and untreated groups were repeated twice.

(3) Upbringing of test plants

The pots subjected to the treatment were covered with a seedling medium, and then cultivated and managed in a glass greenhouse according to the conventional method.

(4) control effect investigation

Two weeks after sowing, seedlings exhibiting the symptoms of hair rot caused by Trichoderma bacteria such as mock decay, forgery, and inhibition of root growth were regarded as seedlings, and the above mathematics was calculated using the average number of seedlings in two repetitions of each treatment. Onset seedling ratio was calculated based on Equation 1. And the control value of the chemical | medical agent treatment tool was computed as a control effect based on the said Formula 2 from the calculated onset rate.

(5) Results

The investigation results are shown in Table 8. As is apparent from Table 8, the disease control agent of the present invention showed a significantly high control effect against rice bran disease caused by Trichoderma bacteria.

According to the present invention, a microbial agent having the following characteristics can be provided.

-Highly effective against various diseases that occur during rice seedlings.

Safe and low environmental impact

There is no or very limited number of uses compared to general chemical synthetic pesticides.

Claims (6)

Gibberella fujikuroi; Pyricularia oryzae; Bacterial rice almond germ (Pseudomonas glumae); Bacterial rice seed germ (Pseudomonas plantarii); Bacterial rice streaks (Pseudomonas avenae); Thala, a filamentous bacterium that has an antagonistic action against pathogens that cause disease during rice seedlings, selected from the genus Fusarium, Rhizopus, and Trichoderma Talaromyces Flavus Y-9401 strain (FERM BP-10642) containing the control agent of the disease caused by the pathogen occurs during the seedling time of rice. delete delete delete delete A process for controlling the disease occurring at the time of rice seedling growth, comprising the step of treating rice seed, seedling, seedling soil or seedling medium with the pest control agent according to claim 1.