WO2007011025A1

WO2007011025A1 - Agent for controlling diseases occurring in the stage of raising rice seedlings

Info

Publication number: WO2007011025A1
Application number: PCT/JP2006/314481
Authority: WO
Inventors: Shinichiro Ogawa
Original assignee: Idemitsu Kosan Co., Ltd.
Priority date: 2005-07-22
Filing date: 2006-07-21
Publication date: 2007-01-25
Also published as: KR20080030102A; TW200738148A; TWI379642B; KR101280679B1; CN101227822A; JP4810151B2; JP2007031294A

Abstract

It is intended to provide a microbial pesticide which exhibits an excellent controlling effect on a number of diseases occurring in the stage of raising rice seedlings, has a high safety and little affects the environment. Namely, an agent for controlling diseases occurring in the stage of raising rice seedlings which contains a fungus belonging to the genus Talaromyces and having antagonism to pathogenic microorganisms inducing diseases occurring in the stage of raising rice seedlings.

Description

Control agent for diseases occurring during the seedling raising season of rice

Technical field

TECHNICAL FIELD [0001] The present invention relates to a control agent for a disease occurring at the time of rice seedling and a method for controlling a disease occurring at the time of rice seedling using the same.

Background art

[0002] Raising healthy and homogeneous seedlings for rice cultivation is one of the most important tasks, but the time of seedling, from germination to seedling, is usually affected by various diseases. Thorough control is carried out with drugs.

[0003] Examples of chemical synthetic agents used for controlling diseases during the rice seedling development period conventionally used are rice seedling disease, rice blast disease, sesame leaf blight, which are seed-borne diseases caused by filamentous fungi. DMI agents such as trifumisol are commonly used for diseases, and oxlinic acid is used for rice seed blight, seedling blight and brown stripes, which are infectious diseases caused by bacteria. Widely used. In addition, rice seedling blight, a soil-borne disease, is controlled with drugs containing hydroxyisoxazole, benomyl and the like.

[0004] However, pathogens having low sensitivity or resistance to the above-mentioned chemically synthesized drugs have emerged and become a problem. In addition, due to concerns about food safety or environmental impact, there is a need to reduce the amount and frequency of chemical synthetic drugs used in crop cultivation. In addition, with the growing demand for food safety, there is a need for the development of technology Z products that can reduce the amount and frequency of use of chemically synthesized drugs even during the seedling season of crops. Specific examples of such techniques include highly safe chemicals that can be used as natural products, food additives, and the like, or agricultural chemicals using living organisms.

[0005] As a disease control technique using living organisms, control using a microbial preparation is known. For example, Patent Document 1 describes a microbial preparation for controlling rice diseases containing Fusarium spp., And Patent Document 2 describes a microbial preparation for controlling rice seedling diseases containing Pseudomonas spp. [0006] In addition, a microbial fungicide, Biotrust (Idemitsu Kosan Co., Ltd.), which contains Talaromyces flavus, which is a type of filamentous fungus, as an active ingredient, has been used for foliage for strawberry anthracnose and Ichigo powdery mildew. It is used as a disease control agent. However, it has not been reported so far that Tallaromyces has an effect on disease control in other crops.

[0007] Patent Document 1: Japanese Patent Application Laid-Open No. 11 89562

Patent Document 2: Japanese Patent Laid-Open No. 2002-17343

Disclosure of the invention

[0008] The present invention has been made from the above viewpoint, and provides a microbial pesticide that is excellent in controlling effect against many diseases occurring during the seedling raising period of rice, is safe and has little environmental impact! This is the issue.

[0009] As a result of intensive investigations to solve the above-mentioned problems, the present inventors have found that filamentous fungi belonging to the genus Tallaromyces have a controlling effect against many diseases that occur during the seedling raising season of rice. Based on these findings, the present invention has been completed.

That is, the gist of the present invention is as follows.

[0011] (1) An agent for controlling a disease occurring during the seedling raising period of rice, comprising a filamentous fungus belonging to the genus Talaromyces having an antagonistic action against a pathogen causing a disease at the time of rice seedling raising.

(2) The control agent for a disease occurring during the rice seedling season as described in (1), wherein the pathogen causing the disease at the rice seedling season is a rice seed infectious pathogen or a soil infectious pathogen.

(3) Rice pathogens (Gibberella lujik uroi); rice blast fungus (Pyriculana oryzae; Cochliobolus miyabea nus);不 (Pseudomonas avenae); Fusarium, a rice seedling blight fungus (Pseudomonas glumae);

Diseases occurring at the time of seedling raising of rice as described in (2), which is at least one of the genus (Fusarium), the genus Pythium, the genus Rhizopus, and the genus Trichoderma Control agent.

(4) The fungus belonging to the genus Talaromyces is Talaromyces' flavus (Talaromyces flavus The disease control agent according to any one of (1) to (3).

(5) The disease control agent according to (4), which is a filamentous fungus belonging to the genus Taralomyces, Talaromyces' Flavus Y-9401 strain (FER M BP-10642).

(6) Rice seedling time, comprising the step of treating rice seeds, seedlings, seedling soil or seedling culture medium with the disease control agent according to any one of (1) to (5) To control diseases that occur in Japan.

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the present invention will be described in detail.

(Disease control agent of the present invention)

The disease control agent of the present invention is a disease control agent that occurs at the time of rice seedling raising. Here, “seedling” means a seedling plant until the planting of rice. Also, “nurturing” means growing seedlings, and “nurturing time” means the cultivation time before planting seedlings. The seedlings at the time of raising seedlings may indicate the growing season of the seedlings by the number of leaves the seedling has, such as the 1-leaf stage, 2-leaf stage, and 3-leaf stage. Speaking of seedling growth time, seedlings at the seedling raising time are seedlings up to about the fourth leaf stage including imperfect leaves, which are the suitable time for transplanting in seedling transplanting cultivation.

[0013] The disease control agent of the present invention contains a filamentous fungus belonging to the genus Tallalomyces as an active ingredient.

(1) Filamentous fungi belonging to the genus Talalomyces

The filamentous fungus belonging to the genus Tallalomyces used in the present invention is not particularly limited as long as it has an antagonistic action against pathogenic bacteria that cause disease during the rice seedling raising season. Here, the “antagonism” means an action of reducing the number of cells of pathogenic bacteria causing disease during the time of seedling raising of the target rice or an action of suppressing the growth, that is, an antibacterial action. As a bacterium having an antagonistic action against a pathogen causing a disease at the time of rice seedling raising, a bacterium belonging to the Talalomyces flavus species is preferable, and among them, Taraguchi Myces' Flavus Y-9401 strain is preferable. Can be mentioned. A mutant strain of Y-9401 strain can also be used as long as it has the above antagonistic action. In the disease control agent of the present invention, one of the above microorganisms can be used alone or in combination of two or more as long as the disease control effect is not affected. [0014] Talalomyces flavus Y-9401 shares were registered on September 2, 1996, at the Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology. (IPOD) (Tsukuba Sakai East Ibaraki Pref. 1-1-1 Central No. 6)) is deposited as FERM IV-15816. It was transferred to an international deposit on 18 July 2006 and deposited as FERM BP-10642.

[0015] The antagonism against pathogenic bacteria causing disease at the time of rice seedling is, for example, arranged at 15 to 35 ° C by placing the test bacteria and pathogenic bacteria causing the disease at the time of rice seedling on the same plate. This can be confirmed by observing the state of growth inhibition of pathogenic bacteria causing disease at the time of rice seedling growth, after culturing for 14 days.

[0016] The filamentous fungus belonging to the genus Tallaromyces used in the present invention can be cultured by the same method as that for ordinary filamentous fungi. For example, filamentous fungi belonging to the genus Tallalomyces can be grown by a liquid culture method such as reciprocating liquid culture cultured in a liquid medium or jafermenter culture or a solid culture method cultured in a solid medium. In order to produce spores of filamentous fungi belonging to the genus Taralomyces with high yield, a solid culture method is more preferably used. As for the culture conditions of filamentous fungi belonging to the genus Talaromyces, the culture temperature that is desirably carried out under aerobic conditions by aeration, stirring, shaking, etc. is preferably 20 to 40 ° C. The culture period is preferably 3 to 60 days, more preferably 3 to 20 days. In addition, it is preferable that the fungal body belonging to the genus Taralomyces used in the present invention is a spore from the viewpoint of storage stability as a product of a disease control agent. Therefore, if necessary, the culture conditions such as the composition of the medium, the pH of the medium, the culture temperature, the culture humidity, and the oxygen concentration at the time of culturing may be used at the end of the culture to sporulate the filamentous fungi belonging to the genus Tallaromyces. It is preferable to prepare so as to adapt to the sporulation conditions.

[0017] A potato dextrose medium, a Sabouraud medium, or the like is used as a liquid medium component used when culturing a filamentous fungus belonging to the genus Talaromyces. When performing solid culture, grains such as rice, wheat, corn, and soybeans, solid components derived from grains such as bran and soybeans, and solid carriers such as clay minerals containing nutrients, if necessary, sugars Or a medium containing a nitrogen source or the like can be used.

[0018] A culture containing a filamentous fungus belonging to the genus Talaromyces may be used as it is, but if necessary Depending on the situation, the culture may be crushed or chopped, and the spore mainly recovered from the culture with a sieve or the like may be used. Alternatively, the cells can be separated from the culture with a liquid such as water or oil, and can be used as is.

[0019] The disease control agent of the present invention is usually 1 X 10 ⁶ to 1 X 10 ¹² cfu / g, preferably 1 X 10 ⁷ to 1 X 10 ^u cfuZg, with filamentous fungi belonging to the genus Talalomyces as a normal colony forming unit. The cultured product or the pulverized product of the cultured product is mixed with various types of preparations suitable for the intended use and usage.

[0020] The disease control agent of the present invention preferably has a content of a culture containing a filamentous fungus belonging to the genus Taralomyces or a pulverized product of the culture of 0.1 to 99.9 mass% 1. 0-50. 0 Quality is more preferred!

[0021] (2) Optional component

In addition to the above-mentioned filamentous fungi belonging to the genus Taralomyces, the disease controlling agent of the present invention can be used by adding as an optional component as long as it does not interfere with the effects of the present invention. Such optional components can be contained as necessary for the purpose of formulation, quality stability and the like.

[0022] As optional components used in the disease control agent of the present invention, for example, one or more of the following components can be used in combination.

[0023] For example, as an extender, kaolin clay, pie-mouth ferrite clay, bentonite, montmorillonite, diatomaceous earth, synthetic hydrous silicon oxide, acid clay, talc, clay, ceramic, quartz, sericite, vermiculite Mineral fine powders such as urelite, perlite, Otani stone, anthracite, limestone, coal stone, zeolite, inorganic compounds such as salt, carbonate, sulfate, nitrate, urea; rice husk, bran, force-shell, shrimp Shell, krill fine powder, rice bran, wheat flour, corn mouth cob, peanut shell, bone meal, fish meal, rice cake powder, wood powder, charcoal, charcoal, bark charcoal, rice husk charcoal, grass charcoal, peat moss, attapulgite, dried Organic fine powder such as animal manure, activated carbon, oil cake, starch and its hydrolyzate; possible D-sorbitol, ratatose, maltose, darcosamine, oligosaccharides, etc. It can be used sexual bulking agent. As the liquid carrier, water, vegetable oil, animal oil, mineral oil, synthetic water-soluble polymer and the like can be used.

[0024] Further, as necessary, casein, gelatin, gum arabic, alginic acid, Natural polysaccharides such as celluloses, carboxymethylcellulose, xanthan gum, chitins, chitosans, etc .; polybulal alcohols; polyacrylic acids; bentonite, etc. are contained as necessary for the purpose of thickening, fixing, dispersing, etc. Can do.

[0025] In addition, dihydric alcohols such as ethylene glycol and propylene glycol may be included as necessary for the purpose of freeze protection.

[0026] Anionic, cationic, and amphoteric surfactants can be included as needed for the purpose of dispersion stability, prevention of aggregation, emulsification, and the like.

(0027) (Method for producing disease control agent of the present invention)

The disease control agent containing a filamentous fungus belonging to the genus Talaromyces of the present invention can be formulated into a form that is actually easy to use. That is, it can be formulated into wettable powders, powders, granules, emulsions, flowables, coating agents, etc., together with various optional components, if necessary, in accordance with a normal preparation method.

[0028] For example, wettable powders and powders can be produced by mixing or pulverizing and mixing the above-described surfactants and quality-stabilizing components as necessary with the above-described solid carrier. .

[0029] For example, a granule is produced by mixing or pulverizing and mixing a surfactant as described above and a component that stabilizes the quality as necessary, and further granulating the solid carrier as described above. can do.

[0030] For example, the emulsion is mixed with a liquid carrier such as vegetable oil, animal oil, mineral oil or the like, for the purpose of emulsifying and dispersing the surfactant as described above, and for the purpose of stabilizing the quality. It can be manufactured by mixing or grinding and mixing.

[0031] For example, the flowable agent is separated from the surfactant as described above for the purpose of thickening the above-mentioned auxiliary agent in water and for preventing freezing of the above-mentioned dihydric alcohol or the like. For the purpose of dispersion, etc., it can also be produced by mixing or grinding and mixing components that stabilize the quality.

[0032] For example, the coating agent can be produced by adding an auxiliary agent to a liquid carrier such as water or oil and mixing them to form a sol or gel.

[0033] (Method for Applying Disease Control Agent of the Present Invention) The disease control agent of the present invention effectively acts to control diseases that occur during the seedling raising period of rice by filamentous fungi, bacteria, microorganism-borne viruses, and the like. In addition, the disease control agent of the present invention effectively acts, for example, on rice seed infectious fungi, and the control of diseases caused by soil infectious fungi. More specifically, the disease control agent of the present invention includes, for example, rice shoot seedlings (Gibberella lujikuroi), 、不不 ¾ ち病 (Pyricularia oryzae, ツネマ集 3 (し ochonbolus miyabeanus), Fir f fungi (Pseudomonas glumae), rice seedlings Pseudomonas plantarn, non-branched stripes;) K ^ KPseudomonas avenae no., Ium rice seedling fungus (Fusarium genus), It is particularly effective in controlling diseases caused by Pythium spp., Rhizopus spp., Trichoderma spp. The disease control agent of the present invention acts on one or more of the above pathogenic bacteria. Among the pathogenic fungi that cause diseases at the time of rice seedling as described above, there are some pathogens that cause disease at times other than the time of rice seedling alone, but here The term `` pathogenic fungus causing disease '' means a pathogenic fungus that causes disease at least during the seedling raising period of rice, and refers to either a pathogenic fungus that causes disease at a time other than the seedling raising season or a pathogenic fungus that does not cause a disease at a time other than the seedling raising time. Is also included.

[0034] The disease control agent containing a filamentous fungus belonging to the genus Talalomyces of the present invention is applied to rice seeds, seedlings, seedling soil or seedling culture medium for the purpose of controlling various diseases as described above. Is appropriately selected depending on the use form such as dosage form and diseases. Examples of such methods include seed soaking treatment, seed dressing treatment, seed application treatment, seed spraying (including spraying) treatment, soil spraying (including spraying) treatment, soil mixing application, soil irrigation application, seedling box Examples include irrigation application, stock application, above-ground liquid application, and above-ground solid application.

[0035] In addition, when applying a disease control agent to rice, a fungicide, an insecticide, a nematicide, an acaricide, a herbicide, a plant growth regulator, a fertilizer, a soil improvement material, or the like is mixed or applied. Alternate application or simultaneous application is also possible.

[0036] The dosage of the disease control agent containing the filamentous fungus belonging to the genus Talalomyces of the present invention cannot be specified unconditionally depending on the type of disease, but for example, in the case of seed soaking treatment It is preferable to apply the preparation as a seed soak solution diluted to 10 to L000 times (mass). The cell concentration is usually 1 X 10 ³ to 1 X 10 ^1Q cfu per ml of soak solution, preferably 1 X 10 ⁴ to 1 X 10 ⁹ cfu.

[0037] In the case of seed dressing treatment, it is preferable to apply 1 to 20% by mass of the preparation with respect to the seed mass. The cell concentration is usually 1 X 10 ³ to 1 X 10 ^1Q per lg seed mass. cfu, preferably 1 × 10 ⁴ to 1 × 10 ⁹ cfu.

[0038] When applying soil spray, it is preferable to apply 50 ~: LOOOml if spraying is liquid per seedling box (for example, about 1800cm ² area) normally used for seedling transplantation. The bacterial cell concentration is usually 1 × 10 ³ to 1 × 10 ^1Q cfu, preferably 1 × 10 ^{4 to} ¹ × 10 ^{1 ()} cfu per ml of sprayed liquid.

[0039] When applied to soil, the seedling box usually used for transplanting seedlings (for example, an area of about 1800 cm ² ) 0.1-1: LOOg is preferred to apply the bacterial cell concentration is soil lml Per 1 X 10 ² to 1 X 10 ⁹ cfu, preferably 1 X 10 ³ to 1 X 10 ⁸ cfu.

[0040] When applying seedling box irrigation, it is preferable to apply 50 to LOOOml if the spraying is liquid per seedling box that is normally used for seedling transplantation (eg, about 1800 cm ² area). The bacterial cell concentration is usually 1 × 10 ³ to 1 × 10 ⁹ cfu, preferably 1 × 10 ^{4 to} 1 × 10 ⁸ cfu per ml of sprayed liquid.

[0041] Although there is no particular limitation on the frequency of application of the disease control agent of the present invention, it is used at a frequency of, for example, 1 to 5 times during rice seedling raising time against pathogenic bacteria that cause disease during rice seedling raising time. By using it, it becomes possible to suppress the occurrence of disease during the time of rice seedling raising.

[0042] Before and after using the disease control agent of the present invention, rice seedlings and rice can be cultivated by a usual method.

Example

[0043] Hereinafter, the present invention will be described in more detail with reference to examples.

Needless to say, the examples are not limited to these examples.

[0044] <Production Example 1>

(Manufacture of spores)

Use bran for the culture medium and add Talaromyces' Flavus Y— 9401 (FERM BP— 106 42) The inoculum of the strain was inoculated and cultured at 30 ° C for 10 days. After completion of the culture, the culture was dried, and the dried culture was sieved to remove the residue of the bran. A taralomyces flavus Y-9401 spore-containing powder (4 × 10 ⁹ cfu / g) was obtained.

<0045> <Formulation Example 1>

(Manufacture of drug product)

Using Talaromyces flavus Y-9401 spore-containing powder obtained in Production Example 1 above, 10% by mass of this spore-containing powder, 5% by mass of SORPOL5082 (manufactured by Toho Chemical Industries) as a surfactant, and clay mineral as an extender (K clay: manufactured by Katsumiyama Mining Co., Ltd.) The raw materials were mixed to a ratio of 45% by mass and dalcosamine (manufactured by Yaizu Suisan Chemical Co., Ltd.), and mixed and pulverized using a mill. Thus, a preparation of Preparation Example 1 (4 × 10 ⁸ cfu / g) was obtained.

(Rice Bone Seedling Control Test)

(1) Preparation of infected seed

Rice seedlings (variety: Akitakomachi) seeds were harvested from paddy rice because of the frequent occurrence of seedling disease in Honda. This seed was used as a seed infected with an idiotic fungus.

[0047] (2) Drug treatment

Dilute the formulation prepared in Formulation Example 1 200 times (mass) with water, and inject 5 g of the seeds infected with B. rot fungus into 15 ml of this solution, and immerse it in an artificial meteor at 30 ° C for 48 hours in the dark. Treated to obtain drug-treated seeds. In addition, the seeds infected with the fungal fungus were immersed in water in the same manner as the target (untreated seeds).

[0048] (3) Growth of test plants

After the above immersion treatment, the seeds infected with the seedling fungus were sown in a 100 cm ² plastic pot filled with soil for raising rice seedlings. The area where the seeds with chemical treatment were sown was designated as the chemical treatment area, and the area where the seeds without treatment were sown was designated as the non-treatment area. Three treatments were performed for both the drug-treated group and the non-treated group. After covering with the above-mentioned paddy rice seedling culture medium, the cultivation was controlled in a glass greenhouse according to a standard method.

[0049] (4) Investigation of control effect

Two weeks after sowing, seedlings with extreme symptom symptoms caused by stupid disease The diseased seedling rate was calculated based on the following (Equation 1) using the average number of diseased seedlings of 3 treatments in each treatment area as diseased seedlings. The calculated diseased seedling rate power was also calculated based on the following (Equation 2) as a control effect in the chemical treatment area.

[0050] [Equation 1]

Diseased seedling rate (%) = number of diseased seedlings / number of all surveyed seedlings X 1 0 0 (Formula 1)

Control value = [(Non-treated plot disease seedling rate-one drug treatment plot disease seedling rate) Untreated plot disease seedling rate] X 1 0 0 (Formula 2)

[0051] (5) Results

The survey results are shown in Table 1. As is clear from Table 1, the disease control agent of the present invention showed a remarkably high control effect against the rice seedling disease.

[0052] [Table 1]

Table 1

(Rice blast control trial)

(1) Preparation of infected seed

In Honda, rice (variety: Akitakomachi) seeds were harvested from rice plants, which had a lot of blast. This seed was used as a seed infected with blast fungus.

[0054] (2) Drug treatment

Dilute the formulation produced in Formulation Example 1 200 times (mass) with water, and inject 4 g of the blast fungus-infected seeds into 15 ml of this solution and immerse it in a dark, 30 ° C artificial meteor for 48 hours. And it was set as the chemical treatment seed. Similarly, the seeds infected with blast fungus were immersed in water and used as the target (untreated seeds).

[0055] (3) Growth of test plants

After the immersion treatment, seeds infected with blast fungus were sown in a 100 cm ² plastic pot filled with soil for raising rice seedlings. The area where the chemical-treated seeds were sown was designated as the chemical-treated area, and the area where the untreated seeds were sown was designated as the non-treated area. Three repetitions were made for both the drug-treated group and the non-treated group. After covering with the above-mentioned paddy rice seedling cultivation soil, the cultivation tube in a glass greenhouse in accordance with a regular method I managed.

[0056] (4) Investigation of control effect

Two weeks after sowing, seedlings with blast lesions appearing on the foliage were used as diseased seedlings, and the average number of diseased seedlings in three iterations of each treatment area was used. Was calculated. The calculated diseased seedling rate was also calculated based on the above (Equation 2) as a control effect in the chemical treatment area.

[0057] (5) Results

The survey results are shown in Table 2. As is clear from Table 2, the disease control agent of the present invention showed a remarkably high control effect against rice blast.

[0058] [Table 2]

Table 2

(Rice blight control trial)

(1) Preparation of infected seed

Rice (variety: Fusaotome) seeds were put into a solution containing a bacterial wilt disease and forcibly inoculated into the seeds under reduced pressure conditions. After air drying, the seed pods were used as seeds infected with bacterial wilt.

[0060] (2) Drug treatment

Dilute the formulation produced in Formulation Example 1 200 times (mass) with water, and inject 5 g of the seeds infected with B. subtilis in 15 ml of this solution, in the dark at 30 ° C for 48 hours. Immersion treatment was performed to obtain drug-treated seeds. The seeds infected with Bacterial wilt were similarly immersed in water to give untreated seeds.

[0061] (3) Growth of test plants

After the soaking treatment, the seeds infected with the bacterial wilt disease were sown in a 100 cm ² plastic pot filled with soil for raising rice seedlings. The area where the seeds treated with the drug are seeded The area where the untreated seeds were sown was designated as an untreated area. Three repetitions were made for both the drug-treated group and the untreated group. After covering with the above-mentioned paddy rice seedling culture medium, the cultivation was controlled in a glass greenhouse according to a standard method.

[0062] (4) Investigation of control effect

Two weeks after sowing, the seedlings with the symptom of bacterial wilt disease such as decay, wilting, and whitening of the seedlings were used as the diseased seedlings, and the average value of the number of diseased seedlings in three iterations of each treatment area was used. Based on Equation 1), the diseased seedling rate was calculated. Based on the above (Equation 2), the calculated control value of the chemical treatment area was also calculated based on the above-mentioned diseased seedling rate power.

[0063] (5) Results

The survey results are shown in Table 3. As is apparent from Table 3, the disease control agent of the present invention showed a remarkably high control effect against bacterial wilt disease.

[0064] [Table 3]

Table 3

(Rice Bacterial Disease Control Test)

(1) Preparation of infected seed

Rice (variety: Fusaotome) seeds were put into a solution containing a bacterial wilt of seedlings and forcibly inoculated into the seeds under reduced pressure conditions. After air-drying, the seed pods were used as seeds infected with bacterial seedlings.

[0066] (2) Drug treatment

Dilute the formulation produced in Formulation Example 1 200 times (mass) in water, and in this solution, add 5 g of seedling-infected bacterial seeds that have been absorbed at 15 ° C for 5 days. In the dark, 30 ° It was immersed in C artificial meteorological device for 24 hours to obtain drug-treated seeds. The seeds with seedling bacteriomycetes infection were similarly immersed in water and used as the target (untreated seeds).

[0067] (3) Growth of test plants After the above immersion treatment, seedlings infected with bacterial wilt of seedlings were sown in a 100 cm ² plastic pot filled with paddy rice seedling culture medium. The area sowed with the drug-treated seeds was designated as the drug-treated area, and the area sowed with the untreated seeds was designated as the untreated area. Three repetitions were made for both the drug-treated group and the untreated group. After covering with the above-mentioned paddy rice seedling culture medium, the cultivation was controlled in a glass greenhouse according to a standard method.

[0068] (4) Control effect investigation

Two weeks after sowing, the seedlings with the symptoms of seedling bacterial disease such as decay, wilting, and whitening of the seedlings were used as diseased seedlings, and the average value of the number of diseased seedlings in three iterations of each treatment area was used. Based on (Equation 1), the diseased seedling rate was calculated. Based on the above (Equation 2), the calculated control value of the chemical treatment area was also calculated based on the above-mentioned diseased seedling rate power.

[0069] (5) Results

The survey results are shown in Table 4. As is apparent from Table 4, the disease control agent of the present invention showed a remarkably high control effect against rice seedling bacterial diseases.

[0070] [Table 4]

Table 4

(Rice brown disease control test)

(1) Preparation of infected seed

Rice (variety: Fusaotome) seeds were put into a solution containing brown streak fungus and inoculated into the seeds under reduced pressure. After air-drying, this seed bud was used as a seed with brown stripe fungus infection.

[0072] (2) Drug treatment

Formulation Example 1 produced as described above was diluted 200 times (mass) in water, and 5 ml of brown streak-infected seeds absorbed in 15 ml of this solution at 15 ° C for 5 days were added to the artificial body at 30 ° C in the dark. It was immersed in a meteorological device for 24 hours to obtain drug-treated seeds. Similarly, the seeds infected with brown streak were immersed in water and used as the target (untreated seeds). [0073] (3) Growth of test plants

After the above immersion treatment, the seeds infected with brown stripe fungus were sown in a 100 cm ² plastic pot filled with paddy rice seedling culture soil. The area sowed with the drug-treated seeds was designated as the drug-treated area, and the area sowed with the untreated seeds was designated as the untreated area. Three repetitions were performed for both the drug-treated group and the untreated group. After covering with the above-mentioned paddy rice cultivation seedling, the cultivation was controlled in a glass greenhouse in accordance with a regular method.

[0074] (4) Investigation of control effect

Two weeks after sowing, the seedlings with the symptoms of brown stripe disease such as rot, wilting and browning of the seedlings were used as the diseased seedlings, and the average value of the number of diseased seedlings in the 3 iterations of each treatment area was used. Based on 1), the diseased seedling rate was calculated. Based on the above (Equation 2), the calculated diseased seedling rate power was also calculated as a control effect in the chemical treatment area.

[0075] (5) Results

The survey results are shown in Table 5. As is apparent from Table 5, the disease control agent of the present invention showed a remarkably high control effect against rice brown stripe disease.

[0076] [Table 5] Table 5

(Test for controlling rice seedling blight with Fusarium fungus)

(1) Inoculation of pathogenic bacteria

The paddy rice seedling culture medium was mixed with Fusuma and the Fusarium fungus culture cultivated in the paddy rice seedling culture medium, to obtain soil contaminated with rice seedling blight by Fusarium fungus.

[0078] (2) Drug treatment

Rice (variety: Koshihikari) was sown in a 100 cm ² plastic pot filled with the above-mentioned soil contaminated with Fusarium fungus. Then, a solution obtained by diluting the preparation prepared in Preparation Example 1 1000 times (mass) with water is uniformly sprayed (25 ml) onto the soil surface, It was. In addition, a pot using the drug solution as water was prepared and used as a target (untreated section). The drug treatment group and the non-treatment group were repeated twice.

[0079] (3) Growth of test plants

The pot subjected to the above treatment was covered with a paddy rice seedling culture medium, and then cultivated and managed in a glass greenhouse according to a standard method.

[0080] (4) Investigation of control effect

Two weeks after sowing, the symptom of seedling blight caused by Fusarium fungi such as decay, wilting, and browning of the seedlings appears, and the average number of diseased seedlings in the two iterations of each treatment area is determined. Based on the above (Formula 1), the diseased seedling rate was calculated. Based on the above-mentioned diseased seedling rate, the control value of the chemical treatment area was calculated as a control effect based on the above (Formula 2).

[0081] (5) Results

The survey results are shown in Table 6. As is apparent from Table 6, the disease control agent of the present invention was remarkably high and effective in controlling rice seedling blight caused by Fusarium fungi.

[0082] [Table 6]

Table 6

(Riceps fungus control test for rice seedlings)

(1) Inoculation of pathogenic bacteria

Rice bran seedling culture medium was uniformly mixed with bran and Rhizopus fungus cultures cultured in paddy rice seedling culture medium, to obtain soil contaminated with rice seedling blight by Rhizopus spp.

[0084] (2) Drug treatment

Rice (cultivar: Koshihikari) was sown in a 100 cm ² plastic pot filled with soil contaminated with seedling blight caused by Rhizopus. Then, a solution obtained by diluting Formulation Example 1 1000 times (mass) with water was uniformly sprayed (25 ml) on the soil surface to obtain a chemical treatment section. In addition, a pot was prepared using the above-mentioned drug solution as water, and used as a target (untreated section). Drug treatment area and nothing Two treatment cycles were used for both treatment areas.

[0085] (3) Growth of test plants

[0086] (4) Investigation of control effect

Two weeks after sowing, symptoms of seedling blight caused by Rhizopus bacteria such as seed rot, wilting, root growth inhibition, etc. developed, and the seedlings were treated as diseased seedlings. Based on the above (Equation 1), the average seedling rate was calculated. Then, from the calculated diseased seedling rate, the control value of the chemical treatment area was calculated as a control effect based on the above (Formula 2).

[0087] (5) Results

The survey results are shown in Table 7. As is apparent from Table 7, the disease control agent of the present invention showed a remarkably high control effect against rice seedling blight caused by Rhizopus.

[0088] [Table 7]

Table 7

(Trial derma bacterium rice blight control test)

(1) Inoculation of pathogenic bacteria

Trichoderma fungus culture cultivated in bran and paddy rice seedling culture medium was uniformly mixed with the paddy rice seedling culture medium to obtain soil contaminated with rice seedling blight by Trichoderma bacteria.

[0090] (2) Drug treatment

Rice (variety: Koshihikari) was sown in a 100 cm ² plastic pot filled with soil contaminated with seedling blight caused by Trichoderma. Then, a solution obtained by diluting Formulation Example 1 1000 times (mass) in water was uniformly sprayed (25 ml) onto the soil surface to obtain a chemical treatment section. In addition, a pot using water as the drug solution was prepared and used as a target (untreated section). The drug treatment group and the non-treatment group were repeated twice. [0091] (3) Growth of test plants

[0092] (4) Investigation of control effect

Two weeks after sowing, symptoms of seedling blight caused by Trichoderma bacteria such as seed rot, wilting, root growth inhibition, etc. developed. Based on (Equation 1) above, the diseased seedling rate was calculated using the average value. Then, the control value of the chemical treatment area was calculated as the control effect from the calculated diseased seedling rate based on the above (Formula 2).

[0093] (5) Results

The survey results are shown in Table 8. As is apparent from Table 8, the disease control agent of the present invention showed a remarkably high control effect against rice seedling blight caused by Trichoderma fungi.

[0094] [Table 8]

Table 8

Industrial applicability

[0095] According to the present invention, a microorganism preparation having the following features can be provided.

• Highly effective against various diseases that occur during the rice seedling season.

• Safe and less environmental impact.

• There are no or very few restrictions on the number of uses compared to general chemical synthetic pesticides!

Claims

The scope of the claims

[1] An agent for controlling a disease occurring at the time of rice seedling, comprising a filamentous fungus belonging to the genus Talaromyces having an antagonistic action against a pathogen causing a disease at the time of rice seedling.

[2] The agent for controlling a disease occurring during the rice seedling raising period according to claim 1, wherein the pathogen causing the disease during the rice seedling raising period is a rice seed infectious pathogen or a soil infectious pathogen.

[3] The pathogen causing disease during the seedling raising season of rice is Gibberella fojikur oi; Pyncuiaria oryzae; Rice bran; Cochiiobolus miyabeanu s Pseudomonas glumae; ィ (Sseed) Bacillus subtilis disease ¾ (Pseudomonas plantarii); Rice brown fungus (Pseudomonas avenae); The agent for controlling a disease occurring during the seedling raising period of rice according to claim 2, which is at least one of the genus Pythium, the genus Rhizopus, and the genus Trichoderma.

[4] The disease control agent according to any one of claims 1 to 3, wherein the filamentous fungus belonging to the genus Talaromyces is Talaromyces flavus.

[5] Fungal power belonging to the genus Talalomyces Talalomyces' flavus Y-9401 strain (FERM

The disease control agent according to claim 4, which is BP-10642).

[6] Rice seedling time, comprising the step of treating rice seeds, seedlings, seedling soil or seedling culture medium with the disease control agent according to any one of claims 1 to 5. To control diseases that occur in Japan.