WO2017188049A1 - Composition for microbial pesticide formulation, method for producing same, and method for use thereof - Google Patents

Abstract

[Problem] To provide: a composition for microbial pesticide formulation in which a microbial pesticide filamentous fungi, which is a pesticide active ingredient having an effect of controlling pests and/or weeds, and of regulating plant growth, is highly activated by acetic acid, said composition for microbial pesticide formulation being able to be stored for long periods; a method for producing same; a method for controlling pests and weeds with the use thereof; and a method for regulating plant growth. [Solution] The abovementioned problem is solved by using a composition for pesticide formulation containing: a microbial pesticide filamentous solid culture medium having one or more selected from grain seeds, the husks, bran or milled seeds thereof as a solid culture medium; and acetic acid.

Description

Microbial pesticide formulation composition, method for producing the same, and method for using the same

The present invention relates to a microbial pesticide preparation composition and the like. More specifically, a fungus having a pesticide, weed, etc. as a pesticide active ingredient that exhibits a control action against pests, weeds, etc., and has improved the control activity of the fungus with acetic acid, and is activated by acetic acid. The highly active microbial pesticide preparation composition that enables long-term survival of the filamentous fungus in a state, that is, excellent in storage stability, its production method, pest and weed control method, and plant growth adjustment method Etc.

Protecting useful plants such as pests and weeds is an indispensable task for efficient agricultural production. Synthetic pesticides are used for this purpose, and they have achieved great achievements. However, in recent years, the emergence of resistant pests caused by multiple administrations of synthetic pesticides and the problem of environmental destruction have been taken up, and how to reduce agricultural burdens efficiently and continuously produce agricultural products in the agricultural field. It has become an important issue.

As one of the solutions, microbial pesticides utilizing the function of microorganisms have been proposed. The use of these microbial pesticides alone or in combination with synthetic pesticides can reduce the environmental impact, The effect of suppressing the appearance frequency of drug-resistant pests and drug-resistant weeds in question is recognized.

Among the useful microorganisms that show pests, diseases, weed control activities, etc. and improve agricultural productivity, there are fungi composed of tubular cells called mycelium, which are collectively called biopesticide filamentous fungi. Various studies have been made on the technology for utilizing this biopesticide filamentous fungus as an agricultural material. For example, Patent Document 1 discloses a disease control agent having a Tallomyces spp. Is a pest control agent or a disease control agent containing a pesticide active as an agrochemical active ingredient, and Patent Document 3 discloses Verticillium spp. A disease control agent comprising an agrochemical active ingredient is disclosed, and Patent Document 4 discloses a weed control agent comprising a Drexella species as an agrochemical active ingredient.

On the other hand, the biopesticide filamentous fungus is improved in controlling activity and the like by being applied simultaneously with acetic acid. For example, Patent Document 5 discloses that Trichoderma spp. An example in which the control activity against is improved is disclosed.

Despite the fact that some of the beneficial effects of the combined use of biopesticide filamentous fungi and acetic acid are known, the knowledge of the composition containing biopesticide filamentous fungus and acetic acid is ad hoc mixed preparation There are no reports of single-packaging pesticide preparations that have been stored on the site for a considerable period of time after being mixed and prepared at the manufacturing stage until use through distribution. I can't find it. In the present invention, the latter is used to distinguish the agrochemical composition, which is an on-site mixture used immediately after preparation, from the agrochemical composition, which is a single package agrochemical formulation as an industrial product scheduled to be stored. It is only referred to as “agrochemical formulation”.

The reason for staying in such on-site mixture is obvious, because acetic acid acts bactericidally against the biopesticide filamentous fungi that are agrochemical active ingredients. As an attempt, when acetic acid was added to an aqueous suspension containing 1 × 10 ¹¹ CFU (Colony Forming Unit) / L of Trichoderma aspereroides SKT-1 strain, the SKT-1 strain was viable within 24 hours after the addition. The fungus is annihilated. A simple mixture of biopesticide filamentous fungi and acetic acid improves control activity in a very short period of time after mixing, but since the bacteria die over time, storage is impossible and storage stability as an industrial product However, it could not be established as an agrochemical formulation composition that is required.

Among these background technologies, in this industry, there has been a strong demand for the development of a formulation technology that does not kill the biopesticide filamentous fungus over time even if it is combined with acetic acid. .

JP 2007-31294 A JP 7-48216 A JP 2006-169115 A Japanese Patent Laid-Open No. 6-277042 JP-A-9-87122

The present invention is a microbial pesticide preparation composition in which a biopesticide filamentous fungus which is an agrochemical active ingredient is highly activated by acetic acid and can be stored for a long period of time, a method for producing the same, a method for controlling pests and weeds by using the composition, plant growth The purpose is to provide adjustment methods.

In order to achieve the above-mentioned object, the present inventors have conducted intensive research, and as a result, biopesticide filamentous fungi using cereal seeds and / or processed seeds (one or more of hulls, straws, and refined products) as a solid medium. The present inventors have found that a microbial pesticide preparation composition containing a solid culture and acetic acid can solve the above problems, and has completed the present invention.

That is, the embodiment of the present invention is as follows.
(1) A biopesticide filamentous fungus solid culture using at least one selected from cereal seeds, its hulls, straws, and refined products as a solid medium, and an agrochemical formulation composition containing acetic acid.
(2) The agrochemical formulation according to (1), wherein the biopesticide filamentous fungus is a filamentous fungus having a controlling action against pests and / or weeds and a plant growth regulating action.
(3) Filamentous fungi having a controlling action against pests and / or weeds and a plant growth regulating action include the genus Beauveria, the genus Coniothyrium, the genus Metarhizium, the genus Talaromyces (Talaromyces), Trichoderma The agrochemical formulation composition according to (2), which is a bacterium belonging to any of the genus Trichoderma) and the genus Verticillium.
(4) The agrochemical formulation according to (3), wherein the filamentous fungus having a controlling action against pests and / or weeds and a plant growth regulating action is Trichoderma sp.
(5) The genus Trichoderma is an Asperelloides inoculum, an Asperellum inoculum, an atroviride inoculum, a hamatum inoculum, a harzianum inoculum, or Koningii (Koningii inoculum) ) Agrochemical formulation composition.
(6) The agrochemical formulation according to (5), wherein the Trichoderma spp. Is Aspereroides sp.
(7) The agrochemical formulation according to (6), wherein the Aspereroides inoculum is Trichoderma aspereloides SKT-1 strain (FERM BP-16510).
(8) The agrochemical formulation according to any one of (1) to (7), wherein 10 to 10 ¹⁰ CFU (Colony Forming Unit) of the biopesticide filamentous fungus is contained in 1 g of the agrochemical formulation composition. Composition.
(9) The agrochemical formulation according to any one of (1) to (8), wherein the solid medium is a refined product of cereal seeds.
(10) The agrochemical composition according to any one of (1) to (9), wherein the cereal is a gramineous cereal.
(11) The agrochemical formulation according to (10), wherein the gramineous grain is barley.
(12) The agrochemical formulation according to any one of (1) to (11), wherein the acetic acid content is 0.01 to 1% by mass, for example, 0.04 to 0.6% by mass in the agrochemical formulation composition. Composition.
(13) The agrochemical composition according to any one of (1) to (12), wherein the composition is a granular agrochemical formulation, and the solid medium is a granule.
(14) The agricultural chemical preparation composition according to (13), wherein the acetic acid is an acetic acid adsorbent obtained by adsorbing acetic acid on a mineral fine powder.
(15) The agrochemical formulation according to (14), wherein the fine mineral powder is hydrous amorphous silicon dioxide.
(16) The agricultural chemical formulation composition according to (14) or (15), wherein the content of the acetic acid adsorbent is 0.5 to 2% by mass in the agricultural chemical formulation composition.
(17) Biopesticide filamentous fungi in which the biopesticide filamentous fungus adheres to the solid medium by solid-culturing the biopesticide filamentous fungus using one or more selected from cereal seeds, hulls, cocoons, and refined products as a solid medium A method for producing an agrochemical formulation composition comprising adding a solid culture of a fungus and then adding acetic acid to the culture.
(18) The method according to (17), wherein the agricultural chemical preparation composition is a granular material, and the solid medium is a granular material.
(19) The acetic acid is supported on the surface of the biopesticide filamentous fungus solid culture by spraying acetic acid or a solution obtained by diluting acetic acid with water or an organic solvent, as described in (17) or (18) Method.
(20) The method according to (18), wherein acetic acid is adsorbed on the fine mineral powder, and the adsorbate is coated on the surface of the biopesticide filamentous fungus solid culture.
(21) The method according to (20), wherein the mineral fine powder is hydrous amorphous silicon dioxide.
(22) Weeds, diseases, characterized by applying the agrochemical formulation composition according to any one of (1) to (16) to plant seedlings, seedlings or seeds and cultivating the plants, One or more control and / or plant growth adjustment methods selected from pests.
(23) One or more control agents selected from weeds, diseases, and pests, characterized in that the agrochemical formulation composition according to any one of (1) to (16) is applied to soil where plants are grown. And / or plant growth regulation method.
(24) The method according to (23), wherein 5 to 1000 g, for example, 100 to 1000 g of the agrochemical composition is applied per 1 m ^{2 of} soil.

According to the present invention, a biochemical pesticide active ingredient, which is a pesticide active ingredient, is highly activated by acetic acid and can be stored for a long period of time. Composition), a method for producing the composition, a method for controlling pests and weeds by using the composition, a method for controlling plant growth, and the like.

The microbial pesticide preparation composition of the present invention is obtained by solid-cultivating agriculturally useful biopesticide filamentous fungi (biological pesticide filamentous fungus solid culture, that is, the cells of cultured biopesticide filamentous fungi (including mycelia and spores). And a mixture of solid medium using grain seeds and / or processed seeds) and acetic acid.

In the present invention, “biopesticide filamentous fungi” refers to agricultural production by controlling pests such as pests and weeds, improving the soil to be suitable for cultivation, and promoting or suppressing the growth of the plant itself. It means a filamentous fungus that improves the property, and is not limited to those having a pesticide registration or those clearly showing the effect as a pesticide. Examples include the genus Acremonium, the genus Alternaria, the genus Ambrosiella, the genus Arthrobotrys, the genus Aspergillus, and the genus Aureobasidium Genus, genus Beauveria, genus Blastomyces, genus Botryospolium, genus Botrytis, genus Chalara, genus Cercospora Cephalosporium, Chrysomnia, Chrysosporium (C Lysosporia, Chlorodome, Cladosporium, Coccidioides, Coniothyrium, Cryptocoun, Cryptococcus , Genus Curvularia, genus Cylindrocarpon, genus Cylindrocladium, genus Drechsella, genus Epicoccum, genus Eupenicilium , Fusarium genus, Geotrica (Geotricum), Gliocladium, Graphium, Helicomyces, Helicosporum, Heliscus, Helmintospo Helminthosporia, Hyalendendron, Hystoplasma, Isaria, Lemoniera, Metarium sp, and Metarium sp. , Monilia genus, Monocillium genus, Mortilella (Mo rtierella spp., Mucor spp., Nigrospora spp., Nodulisporium spp., Nomuraea spp., Oidiodendron spp., Oidium spp. Bacteria, genus Paecilomyces, genus Penicillium, genus Fialomyces, genus Fialophora, genus Pyricularia, Rafaelaria, Rafaelaria ) Genus, Rinocladiella genus, Rhizoctonia nia spp., Sporothrix spp., Staphylotricum spp., Stilbella spp., Tallaromyces spp., Thermomyces spp. Examples include Trichoderma spp., Trichothecium spp., Triposperum spp., Varicospolium spp., Verticillium spp. Any known biopesticide filamentous fungus can be used as long as it has a controlling activity against pests and the like, and the present invention is not limited to the above-exemplified fungi, but among others, the genus Boberia, the genus Coniotylium, the genus Metalidium, Preferably, fungi, Trichoderma spp., Verticillium spp. Are used, and Trichoderma spp. Are particularly preferred. Among these Trichoderma spp., Aspereloides spp., Asperellum spp., Atroviride spp., Hamatum spp., Harzianum spp., Koningii spp. In particular, Aspereroides spp. Is preferred, and among the Aspereroides spp., The Ministry of International Trade and Industry, Institute of Industrial Science, Biotechnology Institute of Technology, Patent Microorganism Depositary Center (currently the National Institute for Product Evaluation Technology Patent Biological Depositary Center) 1997 The bird that was deposited on November 10th as Trichoderma Atrovilide SKT-1 was transferred to an international deposit on February 13, 2017. Derma-Asupereroidesu SKT-1 strain (FERM BP-16510) is particularly preferred.

Although the number of filamentous fungi (viable bacteria and / or spores) in the agricultural chemical formulation composition of the present invention is not specified, it is usually about 10 to 10 ¹⁰ CFU (Colony Forming Unit) per gram of the agricultural chemical formulation composition, preferably Is about 10 ³ to 10 ⁹ CFU, more preferably about 10 ⁵ to 10 ⁸ CFU. The number of filamentous fungi in the agrochemical preparation composition can be measured by a dilution plate method. Examples of the medium used for the dilution plate method include ordinary agar medium, standard agar medium, potato dextrose agar medium, oatmeal agar medium, malt extract agar medium, potato ginseng agar medium, seawater starch agar medium, zapek dox agar medium, zapek yeast Known media such as extract agar medium, MY20 agar medium, Sabouraud / glucose agar medium, Luria / Bertani agar medium, and the like can be mentioned, but are not limited to these examples, and meet the nutritional requirements of the biological pesticide filamentous fungi to be handled The culture medium to be used may be used. In the dilution plate method, modifications such as addition and deletion of medium components and addition and subtraction of the medium components are often carried out for the purpose of optimizing the medium for the measurement target. It's okay. In addition, conditions such as culture temperature and culture period can be arbitrarily set based on the optimum temperature and colony formation rate of the biopesticide filamentous fungus to be handled.

Further, in the agrochemical formulation composition of the present invention, one or more selected from cereal seeds, their hulls, straws, and refined products are used for the solid medium of the biopesticide filamentous fungus solid culture. In the present invention, “cereal” is used in a broad sense, and examples thereof include rice, maize, barley, wheat, rye, oat (oat). , Oat (wild oat), pigeon (adlay), millet (proso millet), foxtail millet, millet, sorghum, finger millet, teilbi te teff, fonio, kodora millet, macomo (manchurian wild rice) and other gramineous grains, soybean, azuki bean, mung bean an), cowpea, common bean, lima bean, peanut, pea, broad bean, lentil, chickpea (chickpea), Scarlet green beans (runner bean), black beans, black beans, mat beans, tepary beans, rice beans, hyacinth bean, horse gram, rose gram (Bambara groundnut), zeocarpa groundnut, pigeon pear, sword bean, standing batter (jack b) an), glass beans, glass beans, cluster beans, square beans, winged beans, carobage, carob, lupine, tamarind and other legumes Examples thereof include grains, and pseudocereal grains such as oleaceae plants such as buckwheat and tartary buckweat, amaranthaceae, and rhododendron plants. Any edible seed mainly composed of starch can be used, and the present invention is not limited to the above-exemplified cereals. Among them, grass and legume grains are preferable, and barley, among legumes and legume grains, One or more of wheat, rice and soybean are particularly preferred. The use of one or more selected from cereal seeds, their hulls, cocoons, and refined products as a solid medium, and the use of a solid culture containing them as a preparation improves the long-term viability of the biopesticide filamentous fungus in the presence of acetic acid. .

The cereal seeds (including those obtained by removing the outer shell such as brown rice and brown wheat) may be used as they are, but the outer shell (such as rice husks) produced when the seeds are removed are refined. You may use the koji which comes out, refined itself, and a mixture of these two or more. In the present invention, the seed coat, cocoon, and refined product may be collectively referred to as “processed seed product”. In addition, the seed refined product is substantially composed of the endosperm of the seed, and the seed refined product and the endosperm accompanied by an embryo, both of which can be used in the present invention and are not particularly distinguished in the present invention. .

Such cereal seeds and processed seeds can be used as they are as a solid medium, and in the present invention, cultivated biopesticide filamentous fungi using cereal seeds and / or processed seeds as a solid medium, The obtained biopesticide filamentous fungus solid culture is used as a raw material for the agrochemical formulation composition together with the medium.

Cereal seeds and their refined products are inherently granular, and the seed husks and pods are inherently amorphous. These granular materials or irregular shaped materials can be pulverized into finer particles or powders (granular pulverized products, powdered pulverized products, etc.). The shape can be used as it is. The dosage form of the agrochemical formulation composition of the present invention is naturally determined depending on the shape of the grain seeds and / or processed seed products to be used, and the granular agrochemical formulation composition, the irregular shape agrochemical formulation composition, or the powdered agrochemical formulation composition. Although it can take an embodiment, it is preferably a granular agrochemical preparation composition, more preferably a granular material having a particle size of 2 mm or more, and therefore, among the seeds and / or processed seeds of the grain, the granular material is preferable. Among them, grain seed refined products can be used particularly preferably.

The culture method of the biopesticide filamentous fungus according to the present invention can be arbitrarily set according to the type of fungus, strain, and the like. And the seed and / or seed processed material of the said grain are used as a solid medium (carrier of a solid medium) in culture | cultivation. For the purpose of improving the growth efficiency, a carbon source, a nitrogen source, inorganic salts and the like may be added. The culture temperature and the culture time can be arbitrarily set, but for example, conditions for culturing at 10 to 40 ° C. for 2 to 30 days are shown. Further, the obtained culture may be appropriately dried.

Furthermore, in the agrochemical formulation composition of the present invention, acetic acid is blended for the purpose of activating the activity of the biopesticide filamentous fungus. Acetic acid promotes mycelial growth of biopesticide filamentous fungi and improves the activity as an agrochemical active ingredient, whereby the agrochemical formulation composition of the present invention achieves a high control effect. The amount of the acetic acid is not particularly limited. However, if the amount of acetic acid is too small, a sufficient hyphal growth promoting action cannot be expected. On the other hand, it may act bactericidal. A suitable amount of acetic acid that is expected to have a sufficient hyphal growth-promoting effect on the biopesticide filamentous fungi and that the biopesticide filamentous fungus is not sterilized is usually about 0.01 to 1% by mass based on the total amount of the agrochemical formulation composition. Preferably it is 0.03-0.8 mass%, More preferably, it is 0.05-0.5 mass%.

Hereinafter, an example of a method of blending acetic acid into the agrochemical formulation composition is shown, but the present invention is not limited to these methods. First, when a culture medium is a powdery substance, for example, acetic acid may be added to a biopesticide filamentous fungus solid culture and further mixed to homogenize the acetic acid in the powdery pesticide preparation composition. On the other hand, when the medium is a granular material, acetic acid may be supported on the surface. As a method for supporting acetic acid on the surface of the granular material, for example, there may be a method in which acetic acid or a solution obtained by diluting an acid with water or an organic solvent is sprayed on the granular material, but first, hydrous amorphous silicon dioxide (white carbon), etc. A method in which acetic acid is adsorbed on a fine powder of mineral and then the acetic acid adsorbate is coated on the surface of a solid medium is preferable. In this case, the long-term viability of the biopesticide filamentous fungus in the presence of acetic acid is further improved. When acetic acid is supported on the surface of the granular material using the fine mineral powder, the amount of the fine mineral powder loaded with acetic acid is not particularly limited, but is usually about 0.2 to 3% by mass based on the total amount of the agricultural chemical formulation composition. The content is preferably 0.5 to 2% by mass, more preferably 0.8 to 1.5% by mass.

As an example of the method for producing the agrochemical formulation composition of the present invention, a biopesticide is obtained by solid-culturing a biopesticide filamentous fungus using one or more selected from cereal seeds, its hulls, cocoons, and refined products as a medium. After obtaining a biopesticide filamentous fungus solid culture in which the filamentous fungus adheres to the solid medium, a production method of adding acetic acid to the culture can be shown. As an example of a more preferable production method, a biopesticide filamentous fungus is solid-cultured using a solid medium that is a grain seed or a refined product of grains, and the cultured biopesticide filamentous fungus adheres to the granular solid medium. After obtaining a solid culture of a biopesticide filamentous fungus to be produced, a production method for supporting acetic acid on the surface of the culture can be shown. As an example of a more preferable production method, a biopesticide filamentous fungus is solid-cultured using a solid medium that is a grain seed or a refined product of a grain seed, and the cultured biopesticide filamentous fungus adheres to the granular solid medium. The production method of coating the surface of the culture with water-containing amorphous silicon dioxide adsorbed with acetic acid after obtaining the solid culture of the biopesticide filamentous fungus to be performed can be shown.

The application method of the agrochemical formulation of the present invention can be appropriately selected depending on the type of plant, type of pest, application location, application time, dosage form, and the like.

The agrochemical formulation composition of the present invention can be applied directly as it is, or diluted with water or a carrier and applied. Application methods include spraying on plant stems and leaves, spraying on plant stocks, spraying on the surface of the soil, soil mixing, soil irrigation, water surface application, seed dressing, application, soaking method, etc. It is not limited to. Furthermore, the agrochemical formulation composition of the present invention can be mixed with other fungicides, insecticides, nematicides, herbicides, plant growth regulators, fertilizers, soil improvement materials, etc. as necessary, applied alternately, or simultaneously. It can also be applied, and in this case, a more excellent effect may be exhibited.

The application place of the agrochemical formulation composition of the present invention can be applied to nurseries, field fields, paddy fields, orchards, hydroponic culture facilities, etc. for cultivating agricultural and horticultural plants, but is not limited thereto.

The application time of the agrochemical formulation of the present invention is not limited during the planting period. In the case of the main field, before planting, at the time of planting, after planting, in the seedling period, any time before sowing, simultaneously with sowing, or after sowing But it can be applied.

The application rate of the agrochemical formulation composition of the present invention varies depending on the type of applied plant, the type of pests and weeds, the condition of the soil, the application time, the planting density, the dosage form, etc. In this case, about 100 to 1000 g per 1 m ² of soil can be used, and in the case of main field, about 5 to 1000 g per 1 m ² of soil can be used. In addition, in the case of dressing seeds (including seeds, tubers, bulbs, bulbs, etc.), the pulverized product can be used directly or diluted with water and used at about 1 to 100 g per 1 kg of seeds. .

Specific examples of plants to be applied with the agrochemical formulation composition of the present invention include cereals (eg, rice, wheat, barley, rye, oat, corn, sorghum, millet, millet, barnyard millet, millet, buckwheat). Potatoes (eg, potato, sweet potato, taro, yam, konjac), beans (eg, soybean, azuki bean, kidney bean, pea, broad bean, groundnut, cowpea, chickpea, yellow bean), vegetables (eg, eggplant, tomato, Peppers, peppers, cucumbers, melons, watermelons, pumpkins, zucchini, shiroi, yugao, tougan, bitter gourd, cabbage, Chinese cabbage, broccoli, cauliflower, radish, turnips, gentian rhinoceros, komatsuna, mizuna, leek, onion, leek, garlic, rakki US Lagas, lettuce, burdock, garlic, buffalo, carrot, honeybee, celery, parsley, strawberry, spinach, okra, perilla, basil, mint, ginger, ginger, fruit tree (eg, apple, pear, pear, quince, quince) , Cherry, peach, plum, ume, apricot, chestnut, walnut, almond, pecan, grape, kiwifruit, akebi, oyster, fig, pomegranate, raspberry, blackberry, blueberry, cranberry, citrus, loquat, olive, bayberry, mango , Guava, avocado, date palm, coconut palm, banana, pineapple, papaya, passion fruit, acerola), special crops (eg cotton, flax, rush, rapeseed, sunflower, sesame, oil palm, sugar beet, sugar cane, tea, coffee, kaka , Hop, tobacco), flower (eg cosmos, morning glory, marigold, spinach, gypsophila, sweet pea, chrysanthemum, carnation, tulip, lily, narcissus, gladiolus, cyclamen, begonia, water lily, dahlia, rose, cymbidium, cattleya) , Turfgrass (e.g., cucumber shiba, himeko ryeba, boar, bermudagrass, bentgrass, fescue, ryegrass, bluegrass), trees (e.g., cherry, azalea, oak, zelkova, cedar, beech) It is not limited to these examples. It can also be applied to plants that have been given resistance to pests, herbicides, and resistance to environmental stresses such as drought by classical breeding methods and genetic engineering techniques.

The agrochemical formulation composition of the present invention has excellent pest control activity and is useful as a fungicide, nematicide, insecticide, herbicide, plant growth regulator or soil conditioner. The effects as fungicides, nematicides, and insecticides are not limited to those directly showing control effects against pathogenic bacteria, nematodes, and pests. For example, fungi, nematodes, insects that are mediators It also includes the control of viral diseases by controlling the above, and the indirect control of suppressing the growth of pests due to the predominance of the biopesticide filamentous fungi in the soil.

Examples of pathogenic bacteria that can be controlled by the agrochemical formulation composition of the present invention include filamentous fungi, actinomycetes, bacteria, viruses, viroids, and the like. Specifically, the genus Ustylago, for example, Barley bare smut fungus (Ustilago nuda), the genus Tilletia (eg, Tilletia caries), the genus Puccinia scab (eg, fungus) (Puccinia recondita), Gymnosporangium spp., For example Gymnosporadium asiaticum, Phacosoporico spp., For example soybean rust Asparagus purple crest fungus (Helicobasidium mom) a), genus Exobasidium, such as Chamochi fungus (Exobasidium vexans), genus Rhizoctonia, such as Rhizoctonia solani, sclerotium Cucumber and soybean white silk fungus (Sclerotium rolfsii), genus Homopsis (eg, cucumber homoposis root rot fungus), Pyrenochaeta (eg, Pyrenochaeta), eg, tomato brown root rot (S) ) Genus fungi, for example, Septoria chr santhemella, Colletotrichum spp., for example, Strawberry anthracnose fungus (Colletotrichum gloeosporioides), Pestalothiopteris spp., for example, Pestarotropis algae spp. (Alternaria solani), genus Botrytis, such as cucumber gray mold fungus (Botrytis cinerea), genus Cercospora, such as sugar beet fungus (Cercospora beticola), Pseudosor spo Pseudocercospora vitis, Paracercospora genus, such as eggplant brown rot (Paracercospora genula), Cercosporella genus, such as cercosporacosporacer ) Genus fungi, such as wheat eye spot disease fungus (Pseudocercosporella herpotriochoides), Corynespora spp., Eg cucumber brown spot fungus (Corynespora casicola), Mycoberosiella spp. Mycovellosiella natrassi, genus Passalora, for example, Tomato leaf mold (Passalora fulva), genus Curvularia, for example rice brown rice (Curvularia spp. ), Fusarium spp., For example, Fusarium oxysporum f. Sp. Lycopersici, Penicillium spp., For example, Penicillium digitatum, Pyricularia, Pirularia spp. Pyricularia oryzae, Verticillium spp., Such as eggplant half-wilt fungus (Verticillium dahlia), Taphrina spp., Peach currant fungus (Taphrina deformans), erum Powdery mildew (Blumeria graminis f. Sp. Tr tici), Erysiphe genus bacteria such as grape powdery mildew (Erysiphe necator), Sphaerotheca genus bacteria such as strawberry powdery mildew (Sphaerotheca aphanis), Podosphaera (Podosphaera) xanthii), Leveillula spp. such as tomato powdery mildew (Leveillula taurica), Caronectria spp. such as soybean black root rot fungi (Calonectria licicola), Diaporte (Diapolet fungus), Diaporthe citri), Gaumanomyces (Gaeumanomyces), for example, wheat stem blight (Gaeumanomyces graminis), Rosellinia (eg, Rosellinia necatrix), Monosporacus (Musporascus), Monosporacus cannonballus, Elsinoe genus, for example Elsinoe factocetii, Cryphonectria genus, for example Cryphonectria paritia parasite, , For example, Botryosphae ia berengeria, genus Venturia, for example, Venturia naschicola, genus Cochliobolus, such as rice sesame leaf blight fungus (Cochliobolus miyabeanus), diploid bacterium D (Didymella broniae), genus Sclerotinia, eg, cabbage sclerotia, Monilia genus, eg, Monilinia frizoius, eg, Monilinia phricus Rhizopus spp. ), Phytophthora spp., For example, Phytophthora sojae, Pythium spp., For example, Phythium spp., Albgo spp. Bacteria (Albgo macrospora), Peronospora (eg Peronospora destructor), Bremia (eg, Bremia lactoporodeporporus), Pseudospora peus For example, Cucumber downy mildew (Pseudoperonospora cubensis), Plasmopara (Plasmopara) genus E.g., Plasmopara viticola, Aphanomyces, e.g., Aphanomyces cochlioides, e.g. Plasmodiophora, e.g., Plasmophora Spongospora spp., For example potato powder scab, Streptomyces spp., Streptomyces spp., Clavibacter tomato, Clavibacter spp. Clavibacter mi higanensis, Pectobacterium, such as potato soft rot, Rhizobium, such as Rhizobium ratobacter, Rhizobium ratobacter Ralstonia solanacerum, Burkholderia genus bacteria, for example, Burkholderia glumae, Acidoborax genus bacteria, for example, Rice brown moss ) Genus, for example lettuce rot fungus Pseudomonas spp. ), Xanthomonas spp., Such as cabbage black rot fungus (Xanthomonas campestris), Frovirus (Furovirus), eg, wheat dwarf virus (SBWMV), Tobamovirus (Tobamovirus), Tobravirus genus such as tobacco stem virus (TRV), Potexvirus genus such as potato X virus (PVX), Carlavirus genus such as carnation latent virus (CaLV), Clini Virus genus, for example, cucurbit yellowing virus (CCYV), genus Bymovirus, eg For example, wheat stripe dwarf virus (WYMV), Potyvirus, eg Potato virus Y (PVY), Alfavirus, eg Alfalfa mosaic virus (AMV), Cucumovirus, eg Cucumber Mosaic virus (CMV), Comovirus genus such as radish mosaic virus (RaMV), Fabavirus genus such as broad bean virus (BBWV), Nepovirus genus such as tomato ringspot virus ( TomRSV), Sadwavirus genus such as Wenzhou dwarf virus (SDV), Carmovirus genus such as Ron Necrotic spot virus (MNSV), Ophiovirus genus such as Tulip microscopic mosaic virus (TMMMV), Tenuivirus genus such as rice stripe virus (RSV), Tospovirus genus For example, tomato yellow wilt virus (TSWV), phytoreovirus genus such as rice dwarf virus (RDV), begomovirus genus such as tomato yellow leaf curl virus (TYLCV), kaulimovirus Name genera such as cauliflower mosaic virus (CaMV), Pospiviroid, such as chrysanthemum dwarf viroid (CSVd) Possible, the present invention is not limited to these examples.
Among the above pathogenic bacteria, in particular, Helicobacterium, Rhizoctonia, Skrerotium, Homopsis, Pyrenoceta, Choletotricum, Fusarium, Verticillium, Caronectinia, Gomanomyces spp., Roselinia spp., Monosporacus spp., Didimela spp., Sclerotinia spp., Rhizopus spp., Phytophthra spp., Aphanomyces spp., Plasmodiophora spp. Genus, Clavibacter, Pectobacterium, Rhizobium, Ralstonia, Frovirus, Tobamovirus, Tobravirus, Vimovirus, Nepovirus, Carmovirus, Offiovirus, etc. Suitable for soil-borne pathogens according to the present invention It can control.

Further, as nematodes that can be controlled by the agrochemical formulation composition of the present invention, specifically, the genus Apherenchoides, for example, Aphlenchodes besseyi, the genus Bursaphelenchus, For example, pinewood nematode (Bursaphelenchus xylophilus), genus Dityrenchus (for example, Imogusaresenchu (destructor), genus globodera, for example potato strode He erodera glycines, genus Meloidogyne, such as sweet potato root nematode (Meloidogyne incognita), genus Pratylenchus, such as Pratylenchus genus, , Genus Tylenchus, for example, Tylenchus semipenetran, and the like, but the present invention is not limited to these examples.

In addition, as pests that can be controlled by the agrochemical formulation composition of the present invention, specifically, grasshopper pests such as the grasshopper (Rusporia lineosa), the cricket family Telegrillus emma, and the vignetting family (Gryllotalpa orientalis), Kobaneinago of acrididae (Oxya yezoensis), migratory locust (Locusta migratoria), My Guratori over grasshoppers (Melanoplus sanguinipes), piggyback grasshopper (Atractomorpha lata) of the piggyback grasshopper family, of pine cricket family Kayakoorogi (Euscyrtus japonicus), of Nomibatta Department Flea grasshopper (Xya japonicus), thrips Eye pests, for example of the thrips family Hirazuhanaazamiuma (Frankliniella intonsa), western flower thrips (Frankliniella occidentalis), yellow tea thrips (Scirtothrips dorsalis), southern thrips (Thrips palmi), green onion thrips (Thrips tabaci), of Kedah thrips family Kakikudaazamiuma ( Ponticulotrips diospyrosi), Haplotrips aculeatus, stink bug pests, for example, Mogania minuta, Aphingidae, Tobiirotsunozemi (Machaerotypus sibiricus), lid Ten leafhopper (Arboridia apicalis) of leafhoppers family, tea Roh green leafhopper (Empoasca onukii), green rice leafhopper (Nephotettix cincticeps), Inazuma leafhopper (Recilia dorsalis), of Hishiunka family Hishiunka (Pentastiridius apicalis), planthoppers Lalodelphax straellatus, Niloparava lugens, White-footed planthopper (Sogataella furcifera), Nymphaeoptera (Nsia nervosa), Red-tailed planthopper (Nsia nervosa) amendaka saccharivora), Red fan gas back (Achilus flammeus of cold wintry wind planthoppers family), tortoiseshell robe (Orosanga japonicus of Hagoromo family), Tobi Gray Ui (Mimophantia maritima of Aobahagoromo family), Nashikijirami of psyllid family (Cacopsylla pyrisuga), Diaphorina citri (Diaphorina citri), Manpidae (Calophya mangiferae), Grape aphid (Daktolosaira vitifoliae), Larixa aphididae (Adelges larvae) tsugae), aphid pea aphids (Acythosiphon pisum), cotton aphids (Aphis gossypis), Aphis spiraecisma, aphid worms ), Wheat beetle (Rhopalosiphum padi), Whitefly of the whitefly family (Aleurocanthus camelliae), Akacanthus spiniferus (Aleurocanthus spiniferus), Tobacco whitefly emisia argentifolii), greenhouse whitefly (Trialeurodes vaporariorum), cotton butterbur scale insect family of giant straw sandals scale insects (Drosicha corpulenta), Iseria scale insects (Icerya purchasi), pineapple mealybug of mealybugs family (Dysmicoccus brevipes), mandarin orange mealybug (Planococcus citri), Pseudococcus comstock, Pseudococcus hornworm (Ceroplastes ceriferus), Pseudococcidae (Acerda Takahashii), Mulculaidae Aonidiella aurantii, Nasimala scale (Diaspidiotus perniciosus), Aphididae (Tropicus), Ternidumarisu, Auriciaceae Azalea (Stephanitis pyrioides), Nasigunbai (Stephanitis nashi), Stink bugs (Eysarcoris aeneus), Rice worms (Laginotomus elongatis) ), Chabae beetle (Plautia crosssota), M. beetle (Megacopta cribraria), M. pteridae (Cavererus moth), Stink bugs (Dysdercus cingulatus), Leptocorisa actina, Leptocorisa chinensis, Lepidoptera helicidae Rhopalus maculatus), bedbug (Cimex lectularis of bed bug family), Coleoptera, for example Scarabaeidae of cupreous chafer (Anomara cuprea), rufocuprea (Anomara rufocuprea), Japanese beetle (Popillia japonica), rhinoceros beetle (Oryctes rhinoceros), Komemushi Department of Tobi Agrootes ogurae, Melanus okinawensi, Melanotus fortumi, Aphididae, Heterobostrychus hamatipennis), Ginkgobill beetle (Stegobium panicum) of the family Asteridae, Nectaraceae (Petinus clavipes), Cocnues of the genus Agrophyceae Carpophilus hempterus, Carabopheles beetle (Ahasverus avena), Carabineidae (Cryptoles ferrugineus) genus v. stis), the beetle, Epilachna vigintioctopunctata (Henosepilachna vigintioctopunctata), Chai Loco Meno mealworm (Tenebrio molitor of Tenebrionidae), red flour beetle (Tribolium castaneum), beans Beetle (Epicauta gorhami of the blister beetle family), gloss of Cerambycid Hada Gomadarakamikiri (Anoplophora glabripennis ), Grape tiger beetle (Xylotrechus pyrrhoderus), pine beetle beetle (Monochamus alternatus), beetle weevil (Callosobchuchus chinensis), potato beetle L. tarsa decemlineata, western corn root worm (Diabrotica virgifera), cucumber moth beetle (Alacophora femorarias), radish beetle (Phaedon brasiumae), physalis weevil (Phylotletolata) Weevil (Hypera postica), Weevil weevil (Listroderes costirostris), Weevil weevil (Euscepes postfascituus), Weevil weevil (Echinocnemus bipunctas), Weevil weevil rhoptrus oryzophylus), weevil of the family weevil (Sitophilus zeamis), shirasoue sushi (Sphenophrus venasis), pine stag beetle (Tomicus pinipacidae), Bark beetle (Lyctus bruneus), fly insect pests, for example, Tipula aino from the family Ganboaceae, Lovebag from the family Fleidae (Plecia neuralcidae) Spring mushroom ( Pnyxia scabiie), soybean flies of the family Frostidae (Asphondylia yushimai), mosquito flies (Aies aegypti), cyprinid moss (Aedes aegypti) (Chironomus oryzae), Chrysops suavis, Tabanas trigonus, Eumerus strigatus, Butterfly, Bactraceae ponica), Mediterranean fruit fly (Ceratitis capitata), leafminer Department of legume leafminer (Liriomyza trifolii), the pea (Chromatomyia horticola), Mugikimoguribae of chloropidae Department (Meromyza nigriventris), cherry fruit fly (Drosophila suzukii of Drosophila family), Drosophila melanogaster (Drosophila melanogaster) , Hydrelia griseola, Hemipodidae (Hippobosca equina), Humperoceae (Parallelpmmasa sasawawee), Onion flies (Delia antiqua), Flies flies (Delia platura), Flies flies flies (Fannia cynariris), Flies flies (Musca domestica), Flies flies (Musca domestica) (Gasterophilus intestinalis), Hypoderma lineatum, Lepidoptera oestrus ovis, Lepidoptera pests, for example, Endocrita sp. sia), Gomafubokutou (Zeuzera leuconotum of Bokutouga family), Mi someone summer fruit moth (Archips fuscocupreanus of Tortricidae), apple Coca summer fruit tortrix (Adoxophyes orana fasciata), oriental fruit moth (Grapholita molesta), Chahamaki (Homona magnanima), soybean pod borer, Leguminivora glycinivorella (Leguminivora glycinivorella ), Cydia pomonella, Eupoecilia abiguella, Brombalina sp. ), Chauminoga (Eumeta minuscula), Nempogon granola, Negatiti vulgaria (Tinea translucens), Nymchigae of the scorpion family (Bucculatrix moth) Golden moth (Phyllononycter lingoniella), Scarlet moth (Phyllocnistis citrella), Scarlet moth (Acroleopsis sapporensis), Stell moth (ell) Yponomeuta orientalis), Argyresthia conjugella, Nokona regalis, Phyramea cereal, Phorimaea opercella, Peach moth (Carposina sasakii), kingfisher Illiberis pruni, moth moth (Monema flavescens), moth (Ancylolomilia japonica sipakiga), s), Cnaphalocrosis medinalisa, African moth (Ostrinia furnacalis), Ni corn borer (Ostrinia nubilalis), Astragalus genus (Citrus moth) Papilio xutus, Pieris rapae, Prunus gnarly, Parnara gutata, Ascotis selenarial, D. Spectabilis, Malacosoma nestrium testaceum, Spruce family shrimp (Agrius convulvali), Arnapseconspersa, Arnapanthiconspersa, Mytah ipsilon, Tamanaginu waiba (Autographa nigrisigna), Helicoverpa armigera, Corn ear worm (Helicoverpa zea), Tobacco budworm (Heliothis virescens d) exigua), Spodoptera litura, bee pests, for example, Arge pagana, Bee family, Aphemus muri, Athaerus Vespa similima xanthoptera, ants of the antaceae (Solenopsis invicta); For example, Lepisma saccharina, Yamatosimi (Ctenolepisma villosa), Cockroachate pests, for example, Cockroachaceae, B. Cannibal termites (Incitermes minor), Cyprids termites (Copttermes formosanus), Termites (Donopterformes formosanus), Pterodoptera, Cicadas Liposcelis corrodens, white-tailed pests, for example, Chrysophyllum dipteridae (Lipeurus caponis), Belgian white-headed lice (Demilinia bovis), L (Pediculus humanus), Canine lice (Linognathus setosus), Phyrus pedis (Pthyrus pubis), plant mites (Penthalus majoli) ( olyphagotarsonemus latus), a kind of Shiramidani of Shiramidani Department (Siteroptes sp. ), Grapes Hime spider mites (Brevipalpus lewisi of Himehadani family), Namikenagahadani (Tuckerella pavoniformis of Kenagahadani family), Anzuakehadani spider mites family (Eotetranychus boreus), citrus red mite (Panonychus citri), Ringohadani (Panonychus ulmi), two-spotted spider mite (Tetranychus urticae), kanzawai (Tetranychus kanzawai), Triticus pini, Scarlet mite (Aculops pelekassi), Pepperid mite (Epitrimerus pylori), Citrus rastroite levora), Dipactus crenatae, Dendracus urticae (Aleuroglyhus ovatus), Phytoid mite (Tyrophagus putrescentiae), Rozne mite (invention) It is not a thing. Among the above-mentioned pests, in particular, soil pests such as thrips pests, Coleoptera pests, flies and butterflies can be suitably controlled according to the present invention.

Examples of the weeds that can be controlled by the agrochemical formulation composition of the present invention include red cloveraceae weeds such as Oenothera erythrosepala, Oenothera laciniata, buttercup weeds such as Togenino fox ranunculus Ranunculus sardous, terrestrial weeds such as buckwheat (Polygonum convolvus), sanaetadegi (Polygonum raspiforum), Polygonum sap (um) Shi (Rumex obtusifolius), Japanese knotweed (Poligonum cuspidatum), Pennsylvania smart weed (Polygonum pensylvanicum), knotweed (Persicaria longiseta), Ooinutade (Persicaria lapathifolia), Tanisoba (persicaria nepalensis), Portulacaceae weeds such purslane (Portulaca oleracea), dianthus Family weeds such as chickweed (Stellaria media), Dutch tiger rush (Cerastium glomeratum), Stellaria alsine, large clover (Spergula arvensis), cow chickweed (Stell) laria aquatica), red crustaceae weeds such as white cherry (Chenopodium album), broomweed (Kochia scoparaia), red crow (Chepopodium album), red crow (Chepopodium album), red crow hybridus, Amaranthus palmeri, Amaranthus spinosus, Amaranthus rubis, Amaranthus albus, Athus amur s lividus), cruciferous weeds, such as wild radish (Raphanus raphanistrum), black pepper (Sinapis arvensis), cappella bursula rasinus (Lepidium vines), (Rorippa indica), Red-headed burdock (Rorippa islandica), Oyster firefly (Sisymnrium officinale), Red-footed hornet (Cardhamine flexuraosa), Naturturium officina emorosa), legume weeds, such as Sesbania exaltata, Cassia obtusinfolia, Desmodium totuosum, Desmodium totuosum, ), Vulgaria pea (Vicia hirsuta), Yakusou (Kumerowia straitata), Eurasian coconut (Medicago polymorpha), Carrion pea (Vicia angustifolia), Kusanane (Aeschinidiae, Aeschinidae) long theophrasti, Sida spinosa, violet family weeds such as field pansies (Viola arvensis), wild pansies (Viola tricolor), weeping weeds (eg Galium aparinaceae) Ipomoea hederacea), Ipomoea purpurea (Ipomoea purpurea), tall American morning glory (Ipomoea hederacea var integriuscula), beans morning glory (Ipomoea lacunosa), field bindweed (convolvulus arvensis), Noasagao (Ipomoea indica), Ipomoea coccinea (Ipomoea c ccinea, Ipomoea triloba, Lamiaceae weeds, such as Lamium puruumum, Lamum ampurisau, L. Physalis angula (Physalis angulata), American dogwood (Solanum americanum), Barnacle beetle (Solanum carolinense), scorpionaceae weeds, for example, Veronica persica (Vernica persica), Sabasou (Veronica hederaefolia, Asteraceae weeds, for example Eclipta prostrata (Eclipta prostrata), Bidens Tripartita (Bidens tripartita), cocklebur (Xanthium pensylvanicum), wild sunflower (Helianthus annuus), chamomile (Matricaria chamomilla), Inukamitsure (Matricaria perforataorinodora), corn marigold (Chrysanthhemum segetum), Koshikagiku (Matricaria matricarioides), Ragweed (Ambrosia artemisifolia), Obraxa trifida (Ambrosia trifida) igeron canadensis), mugwort (Artemisia princeps), goldenrod (Solidago altissima), dandelion (Taraxacum officinale), chamomile beetle (Anthemis cotula), Ezo Roh fox thistle (Breea setosa), Carduus (Sonchus oleraceus), Jerusalem artichoke (Helianthus tuberosus), Black thistle (Cirsium arvense), Bidens frontosa (Bidens pilosa), cornflower (Centurea cyanus), American thistle (Cirsium vulgares) L tuca scariola, Rudbeckia hirta, Greater Redhorn, Rudbeckia lacinia, Rudbeckia lacinia var. hortensis Bailey), Senecio vulgaris (Senecio vulgais), milk thistle (Silybum marianum), Sonchus Asper (Sonchus asper), field sow thistle (Sonchus arvensis), Hosobaokahijiki (Salsola kali), Naruto ragwort (Senecio madagascariensis), Coreopsis lanceolata (Coreopsis lanceolata) Purple weeds, for example Myosotis arvensis, moth family weeds, for example Asclepias syriaca, Euphorbiaceae weeds, for example Euphorbia helioscopia lata), Enokirusha (Acalypha australis), Aspergillus weeds, for example, American spider (Geranium carolinianum), Oxalis weeds, such as Oxalis corymbosa, Ulridae, S. (Echinochloa oryzicola), Azegaya (Leptochloa chinensis), Chigosasa (Isachne globosa), Paspalum distichoza (Lessia saisau) -Galli), Enocorosa (Setaria viridis), Aquinoenokorogusa (Setaria Faberi), Eurasian burrows (Digitaria esugalis), Eurasiania urus (European urus) Sorghum haremense, Agropyron repens, Bromus tectorum, Cynone dactylum, Panicum dichotomiflorum Texas Ken (Sorghum vulgare), foxtail (Alopecurus geniculatus), darnel (Lolium multiflorum), rigid ryegrass (Lolium rigidum), Setaria pumila (Setaria glauca), beckmannia syzigachne (Beckmannia syzigachne), commelinaceae weeds, for example dayflower (Commelina communis), Ibokusa ( Murdannia keisak), Toxaceae weeds such as Equisetum arvense, Poppy weeds such as Papaver rhoeas, Cyperaceae weeds such as Cyperus iriat Masuge (Cyperus esculentus), smallflower umbrellaplant (Cyperus difformis), bulrush (Scirpus hotarui), Eleocharis acicularis (Eleocharis acicularis), Cyperus (Cyperus serotinus), water chestnut (Eleocharis kuroguwai), fimbristylis miliacea (Fimbristylis miliacea), Hinagayatsuri (Cyperus flaccidus), Azegayatsuri ( Cyperus globosus, Sirpus juncoides, Shirapus wallicii, Sirpus nipponicus, Fenbritsalis autumnalis (Scirpus tabernaemontani), Scrophulariaceae weeds, for example, false pimpernel (Lindernia procumbens), Amerikaazena (Lindernia dubia), Abunome (Dopatrium junceum), Ooabunome (Gratiola japonica), Azetougarashi (Lindernia angustifolia), LIMNOPHILA SESSILIFLORA (Limnophila sessiliflora), Lythraceae weeds, For example, Rica indica
, Ammannia multiflora Roxb (Ammannia multiflora), elatinaceae weeds, for example Elatine triandra Schk (Elatine triandra), Monochoria (Monochoria vaginalis), Monochoria korsakowii (Monochoria korsakowii), the United States vaginalis (Heteranthera limosa), Alismataceae weeds, for example arrowhead (Sagittaria pygmaea), Alismataceae ( Sagittaria trifolia, Alasma canalicatum, Akinashi (Sagittaria aginashi), Weevil weeds, such as Potamogeton distinctus, E. um), Umbelliferae weeds such Seri (Oenanthe javanica) and the like can be mentioned, the present invention is not limited to these examples.

Furthermore, by the agricultural chemical formulation composition of the present invention, for example, land for industrial facilities such as bank slopes, riverbeds, road shoulders and slopes, railway floors, park green spaces, grounds, parking lots, airports, factories and storage facilities, It can weed a wide range of weeds that occur in non-agricultural lands where it is necessary to control the growth of weeds, such as fallow land, or closed land in the city, or in orchards, pastures, lawns, and forestry areas. The pesticide preparation composition of the present invention can also be used as a target for control in rivers, waterways, canals, reservoirs, and the like, for example, Shara spiders (Chara braunii), Duckweeds such as Spirodella polyrhiza, Thais such as Ginkgo biloba. (Ricciocarpus natans), Hosimidroaceae, for example, Spirogyra arcura, Mizuaoii, for example, Eichhornia crassipes, Salamander, for example, Azolla sect. For example, button duckweed (Pistia s Ratiotes), haloragaceae, eg Myriophyllum aquaticum (Myriophyllum aquaticum) and the like can be mentioned, the present invention is not limited to these examples.

In the present invention, the “biopesticide filamentous fungus solid culture” and the “acetic acid adsorbate” both specify the structure simply by indicating the state, and the product is specified by the manufacturing method. Absent. In addition, it is excessively expensive and time-consuming to perform the work and analysis for specifying the structure of these objects other than those described above.

Hereinafter, examples of the present invention will be described. However, the present invention is not limited to these examples, and various modifications can be made within the technical idea of the present invention.

(Test of the effect of promoting the growth of mycelium by acetic acid in various fungal spores)
Mineral salts medium which acetic acid was predetermined concentration added (0.2 wt% NaNO _3, 0.1 _wt% KH ₂ PO 4, 0.02 wt% KCl, 0.02 _wt% MgSO 4 · _7H 2 O) A test medium was used. For each filamentous fungus of Trichoderma aspereloides SKT-1, S. Trichoderma harzianum T-22, and Trichoderma hamatum NBRC 31932, 0.5 ml of spore suspension (1 × 10 ⁶ spores / ml) was added to the test medium. A 100 ml Erlenmeyer flask containing 50 ml was inoculated and cultured with shaking (27 ° C., 120 rpm, 3 days). After the culture, the mycelial growth of each strain was objectively investigated. The test results are shown in Table 1.
Similar tests were also performed on the Talalomyces flavus SAY-Y-94-01 strain and the metalidium anisoprie SMZ-2000 strain.
The Trichoderma aspereloides SKT-1 strain is sold as an agrochemical under the trade name Ecohope (registered trademark, manufactured by Kumiai Chemical Industry Co., Ltd.). Trichoderma harzianum T-22 strain is sold as a soil conditioner under the trade name of Trichodesoil (registered trademark, manufactured by Arista Life Science Co., Ltd.). Trichoderma hamatum NBRC 31932 shares were sold by National Institute of Technology and Evaluation. Talalomyces flavus SAY-Y-94-01 is marketed as an agrochemical under the trade name Tough Block (registered trademark, manufactured by Idemitsu Kosan Co., Ltd.). Metalidium anisoprie SMZ-2000 strain is marketed as an agrochemical under the trade name of Pirate Granules (registered trademark, manufactured by Arista Life Science Co., Ltd.).

As a result, Trichoderma spp. Spores can no longer be observed at an acetic acid concentration of about 0.15 to 0.3% by mass. In other words, even if spores are grown, it is difficult to grow by simply mixing acetic acid above a certain concentration. It was shown that. In addition, the spores of Talalomyces flavus SAY-Y-94-01 and Metalidium anisoprie SMZ-2000 have almost the same results as Trichoderma sp., Although there are some differences in mycelial growth at each acetic acid concentration. It was.

(Storage stability test of agrochemical formulation)
As an agrochemical preparation composition containing acetic acid, acetic acid bulk powder coating preparation and acetic acid direct injection preparation as a product of the present invention, and SKT-1 stock solution (trade name: “Eco-Hope”, manufactured by Kumiai Chemical Industry Co., Ltd.) as acetic acid 3 preparations of 15% by volume were prepared. The acetic acid bulk powder coating preparation was prepared by adsorbing acetic acid to white carbon to give 15% by weight of bulk powder, 8 g of SKT-1 strain barley seed medium culture (barley seed (variety: Hayadri 2)), YG medium (0.3 mass) 0.5 ml of SKT-1 strain spore suspension (1 × 10 ⁶ spores / ml) was inoculated into a 100 ml Erlenmeyer flask containing 8 ml of 2% yeast extract and 2.0% by weight glucose, and allowed to stand at 25 ° C. for 8 days. After standing and then air-dried overnight (hereinafter also referred to as SKT-1 strain solid culture): by mixing well in a plastic bag at a ratio of the above bulk powder = 99: 1 (mass ratio) Covered. The acetic acid direct-injection preparation was prepared by diluting acetic acid in water to give a 15% by mass solution, and the SKT-1 strain solid culture: the above-mentioned solution = 99: 1 (mass ratio) in a SKT-1 strain in a plastic bag. Acetic acid was adsorbed by spraying the solution while shaking the solid culture. After preparation, the preparation was packed in an aluminum bag and stored at 5 ° C. After 0 days (initial value), 1 day and 7 days later, the viable cell count in the preparation was measured by a dilution plate method. The test results are shown in Table 2.

As a result, the filamentous fungus solid-cultured preparation and the acetic acid direct-injection preparation of the filamentous fungus solid culture according to the present invention survive to a certain extent even after 7 days of manufacture, whereas the filamentous fungus solution simply contains acetic acid. In the comparative product in which the mixture was mixed, the number of viable bacteria immediately after production (initial value) was smaller than that of the product of the present invention, and the survival of the filamentous fungus was not already observed one day after production.

(Cucumber white silkworm disease control effect test of agricultural chemical formulation composition)
After pre-culturing cucumber white silkworm (Sclerotium rolfsii) in PDA plate medium, the bacterial agar pieces are mixed with soil bran medium (2.5 kg sand loam, 2.0 kg bran, 3 g yeast extract, 20 g glucose, 1 L distilled water). And cultured at 27 ° C. for 7 days. 1: 5 (volume ratio) of this inoculation source and soil (sterilized sand loam soil: horticultural culture soil (trade name: “Kumiai Nippi Horticulture Culture No. 1”, manufactured by Nippon Fertilizer Co., Ltd.) = 1: 1 (volume ratio)) Contaminated soil was obtained by mixing well at a ratio and leaving it in a moist chamber (27 ° C., dark place) overnight. About 15 ml of this contaminated soil was inoculated by spreading on the stock of a cotyledon stage cucumber (variety: Nissho) that was raised in a plastic cup (diameter 6 cm). After inoculation, the acetic acid bulk powder coating preparation and direct acetic acid injection preparation of Example 2 and SKT-1 strain solid culture were sprayed at 500 g / m ² equivalent to the cucumber stock, and the SKT-1 stock solution was the SKT-1 strain. Liquid: Water = 1:14 (mass ratio) diluted spray solution is irrigated with 3 L / m ² equivalent to the cucumber stock, Torcrolofos methyl wettable powder (trade name: “Risolex wettable powder” (Registered) Trademark), manufactured by Sumitomo Chemical Co., Ltd., irrigated 3 L / m ² of cucumber stock with a spray solution diluted at a ratio of torquelophosmethyl wettable powder: water = 1: 999 (mass ratio). After the treatment with the chemical, it was managed in a moist chamber (27 ° C., dark place) for 3 days, and then the presence or absence of disease was investigated, and the control value was calculated by the following formula. The test results are shown in Table 3.

Control value = (1-disease rate in treated area / disease rate in untreated area) x 100

As a result, the acetic acid bulk powder coating preparation and the acetic acid direct-injection preparation of the product of the present invention exhibit the same degree of control effect as that of Torcrolofosmethyl wettable powder, which is an organophosphorus fungicide, against cucumber silkworm, and It was revealed that the control effect was higher than that of the SKT-1 strain solid culture or the SKT-1 strain solution.

(Cucumber white silkworm disease control effect test by the difference in the processing amount of the agricultural chemical formulation composition)
By the method similar to Example 3, the cucumber silkworm disease control effect test of the acetic acid bulk powder coating preparation was implemented. In addition, the acetic acid bulk powder coating preparation application amount was carried out in double the amount of Example 3. Table 4 shows a comparison of the test results with the data of Example 3.

As a result, a sufficient control effect against cucumber silkworm disease was observed even when the treatment amount of the acetic acid bulk powder coating preparation of the present invention was doubled, but no improvement in the treatment amount-dependent control effect was observed. It was.

(Storage stability due to the difference in the amount of acetic acid in the agricultural chemical formulation, cucumber white silkworm prevention)
Acetic acid is adsorbed on white carbon to make 50%, 15% or 5% by mass of the bulk powder, and the SKT-1 strain solid culture: the bulk powder = 99: 1 (mass ratio) Were mixed well to prepare acetic acid bulk powder coating preparations having an acetic acid content of 0.5% by mass, 0.15% by mass or 0.05% by mass, respectively. After storage at 4 ° C. for 1 week, the number of viable bacteria in the preparation and the cucumber silkworm disease control effect were investigated. The number of viable bacteria in the preparation was examined by the same method as in Example 2, and the cucumber silkworm disease control effect was examined by the same method as in Example 3. Table 5 shows the test results of the number of viable bacteria in the preparation, and Table 6 shows the test results of the cucumber silkworm disease control effect.

As a result, when the acetic acid content is 0.05 to 0.5% by mass, there is no significant decrease in the number of bacteria during storage, and the cucumber silkworm disease control effect is comparable to that of Torquelofosmethyl wettable powder. Became clear.

(Control effect of cucumber seedling blight (Rhizoctonia spp.) Of agricultural chemical formulation composition)
Cucumber seedling blight fungus (Rhizotonia solani AG-4) was pre-cultured on a PDA plate medium, and the agar-containing agar pieces were mixed with soil bran medium and cultured at 27 ° C. for 7 days. This inoculation source and soil (sterilized sand loam soil: horticultural soil = 1: 1 (volume ratio)) were mixed well at a ratio of 1:50 (volume ratio) and left overnight in a humid room (27 ° C., dark place). The soil was contaminated soil. A square bowl (9 cm square) was filled with 200 ml of sterile river sand and 100 ml of contaminated soil in this order. In addition, the non-inoculated section was filled with 100 ml of a mixture of soil bran medium: soil = 1: 50 (volume ratio) instead of contaminated soil. Soil incorporation of acetate bulk powder coating formulation of Example 2 (acetate amount 0.15%) is filled before, contaminated soil 100ml and acetic bulk powder coating formulation 8.1 g (1000 g / ^{m 2} corresponds to the contaminated soil to the corner pots ) Was mixed in a plastic bag, filled into a square bowl, watered, and then sown with cucumber (variety: Nissho). The surface layer spraying of the acetic acid bulk powder coating preparation was carried out by spraying cucumber seeds on the contaminated soil after irrigation and then uniformly spraying 500 g / m ² equivalent on the soil surface layer. As a comparison, 3 L / m ^{2 of} spray solution diluted at a ratio of torquelofos methyl wettable powder: water = 1: 499 (mass ratio) was subjected to soil irrigation treatment before sowing. After the drug treatment, it was allowed to stand overnight in a wet room (27 ° C., dark place) and then managed in a greenhouse (overhead irrigation). Eight days after the inoculation, the index was investigated according to the criteria of the following disease index, and the disease severity, corrected disease severity and control value were calculated according to the following mathematical formula. The test results are shown in Table 7.

<Disease index>
0: No disease 1: Growth abnormality 2: Withering or unbudding

Disease severity = Σ (number of diseased seedlings by index × index) / (number of surveyed seedlings × 2) × 100
Corrected disease severity = average disease severity in each zone−average disease severity control value in non-inoculated zone = (1−corrected disease severity in treated zone / corrected disease severity in untreated zone) × 100

As a result, the powdered acetic acid powder preparation of the present invention exhibits the same level of control effect as that of tolcrofosmethyl wettable powder in both soil admixture treatment and soil surface treatment for cucumber seedling blight (Rhizoctonia). Became clear.

(Effects of cucumber seedling blight (Pisium fungus) control of agrochemical formulation)
A cucumber seedling fungus (Pythium aphanidermatum) was pre-cultured on a PDA plate medium, and the agar-containing agar pieces were mixed with a soil bran medium and cultured at 27 ° C. for 7 days. This inoculation source and soil (sterilized sand loam soil: horticultural soil = 1: 1 (volume ratio)) were mixed well at a ratio of 1: 300 (volume ratio) and left overnight in a wet room (27 ° C., dark place). The soil was contaminated soil. A square bowl (9 cm square) was filled with 200 ml of sterile river sand and 100 ml of contaminated soil in this order. In addition, the non-inoculated section was filled with 100 ml of a mixture of soil bran medium: soil = 1: 300 (volume ratio) instead of contaminated soil. The soil mixing of the acetic acid bulk powder coating formulation of Example 2 (acetic acid content 0.15% by mass) was performed before filling the contaminated soil into a bowl, 100 ml of contaminated soil and 4.1 g of acetic acid bulk powder coating formulation (500 g / m ^2). The mixture was mixed in a plastic bag, filled into a square bowl, watered, and then sown with cucumber (variety: Nissho). Hydroxyisoxazole solution (trade name: “Tatigalen solution” (registered trademark), manufactured by Mitsui Chemicals Agro Co., Ltd.) was diluted with a ratio of hydroxyisoxazole solution: water = 1: 499 (volume ratio) 3 L / spray solution and soil drench treatment m ² corresponds to the after seeding. After the chemical treatment, it was allowed to stand overnight in a wet room (27 ° C., dark place) and then managed in a greenhouse. 12 days after the inoculation, the index was investigated according to the criteria of the following disease index, and the disease severity, the corrected disease severity and the control value were calculated by the following mathematical formulas. The test results are shown in Table 8.

<Disease index>
0: No disease 1: Growth abnormality 2: Withering or unbudding

Disease severity = Σ (number of diseased seedlings by index × index) / (number of surveyed seedlings × 2) × 100
Corrected disease severity = average disease severity in each zone−average disease severity control value in non-inoculated zone = (1−corrected disease severity in treated zone / corrected disease severity in untreated zone) × 100

As a result, it was clarified that, when the acetic acid bulk powder coating preparation of the present invention was mixed with soil, it exhibited the same level of control effect as that of hydroxyisoxazole solution against cucumber seedling blight (Pisium). It was.

(Control effect of cucumber white silkworm by the difference of various cereal culture media of agricultural chemical formulation composition)
Rice grains (variety: Kinuhikari), wheat seeds (variety: Norin 61), barley seeds (variety: Hayadori 2), soybean seeds (variety: Enrei), rice husks, wheat bran It was. SKT-1 strain spore suspension (1 × 10 ⁶ spores / ml) 0.5 ml in a 100 ml Erlenmeyer flask containing 8 g of various grains and 8 ml of YG medium (0.3 mass% yeast extract, 2.0 mass% glucose) Was inoculated and statically cultured at 25 ° C. for 8 days. After culturing, after air-drying overnight, a SKT-1 strain culture and a bulk powder containing acetic acid adsorbed on white carbon and containing 15% by mass of acetic acid at a ratio of 99: 1 (mass ratio) The mixture was coated by mixing well to prepare an acetic acid bulk powder coating preparation. About these preparations, the cucumber silkworm disease control effect was investigated by the same method as Example 3. The test results are shown in Table 9.

As a result, the SKT-1 strain solid culture obtained by using various grains as a solid medium and the preparation containing acetic acid all show the same or better control effect against cucumber silkworm against Torquelofosmethyl wettable powder. Became clear.

The summary of the present invention is as follows.

INDUSTRIAL APPLICABILITY The present invention is a microbial pesticide formulation composition in which a biopesticide filamentous fungus, which is a pesticide active ingredient having a control action against pests and / or weeds and a plant growth regulating action, is highly activated with acetic acid, and can be stored for a long period of time. An object of the present invention is to provide a microbial pesticide preparation composition, a method for producing the same, a method for controlling pests and weeds by using the composition, a method for controlling plant growth, and the like.

By using a biopesticide filamentous fungus solid culture in which one or more selected from cereal seeds, their hulls, cocoons, and refined products are used as a solid medium, and an agrochemical formulation composition containing acetic acid, To solve.

The accession numbers of microorganisms deposited internationally in the present invention are shown below.
(1) Trichoderma aspereloides SKT-1 strain (FERM BP-16510).

[Supplement under rule 26 02.05.2017]

Claims (24)

1. A bio-pesticide filamentous fungus solid culture in which at least one selected from cereal seeds, its hulls, straws, and refined products is used as a solid medium, and an agrochemical formulation composition containing acetic acid.
2. The agrochemical formulation composition according to claim 1, wherein the biopesticide filamentous fungus is a filamentous fungus having a controlling action against pests and / or weeds and a plant growth regulating action.
3. Filamentous fungi having a controlling action against pests and / or weeds and a plant growth regulating action include the genus Beauveria, the genus Coniothyrium, the genus Metalaridium, the genus Talaromyces, the genus Trichoderma The pesticidal composition according to claim 2, which is a bacterium belonging to any one of the genus Verticillium.
4. The agrochemical formulation composition according to claim 3, wherein the fungus having a controlling action against pests and / or weeds and a plant growth regulating action is Trichoderma spp.
5. The genus Trichoderma is an inoculum of Aspereloides, an inoculum of Asperellum, an atroviride, an inoculum of hamatum, or an inoculum of Koningii Agrochemical formulation composition.
6. 6. The agrochemical formulation composition according to claim 5, wherein the Trichoderma spp. Is Aspereroides sp.
7. 7. The agrochemical formulation composition according to claim 6, wherein the Aspereroides inoculum is Trichoderma aspereloides SKT-1 strain (FERM BP-16510).
8. The agrochemical formulation composition according to any one of claims 1 to 7, wherein 10 to 10 ¹⁰ CFU (Colony Forming Unit) of the biopesticide filamentous fungus is contained in 1 g of the agrochemical formulation composition.
9. The agrochemical composition according to any one of claims 1 to 8, wherein the solid medium is a refined product of cereal seeds.
10. The agrochemical formulation according to any one of claims 1 to 9, wherein the cereal is a gramineous cereal.
11. The agricultural chemical formulation composition according to claim 10, wherein the gramineous grain is barley.
12. The agricultural chemical formulation composition according to any one of claims 1 to 11, wherein the acetic acid content is 0.01 to 1% by mass in the agricultural chemical formulation composition.
13. 13. The agrochemical formulation composition according to any one of claims 1 to 12, which is a granular agrochemical formulation composition, wherein the solid medium is a granular material.
14. The agricultural chemical formulation composition according to claim 13, wherein the acetic acid is an acetic acid adsorbate obtained by adsorbing acetic acid on a fine mineral powder.
15. The agrochemical composition according to claim 14, wherein the mineral fine powder is hydrous amorphous silicon dioxide.
16. The agricultural chemical formulation composition according to claim 14 or 15, wherein the content of acetic acid adsorbate is 0.5 to 2% by mass in the agricultural chemical formulation composition.
17. Biopesticide filamentous fungi solid culture using one or more selected from cereal seeds, their hulls, persimmons, and refined products as a solid medium, and the cultured biopesticide filamentous fungus adheres to the solid medium A method for producing an agrochemical formulation comprising adding acetic acid to the culture after obtaining the product.
18. The method according to claim 17, wherein the agricultural chemical preparation composition is a granular material, and the solid medium is a granular material.
19. The method according to claim 17 or 18, wherein acetic acid is supported on the surface of the biopesticide filamentous fungus solid culture by spraying acetic acid or a solution obtained by diluting acetic acid with water or an organic solvent.
20. The method according to claim 18, wherein acetic acid is adsorbed on the fine mineral powder, and the adsorbate is coated on the surface of the biopesticide filamentous fungus solid culture.
21. The method according to claim 20, wherein the mineral fine powder is hydrous amorphous silicon dioxide.
22. A composition selected from the group consisting of weeds, diseases, and pests, wherein the agrochemical preparation composition according to any one of claims 1 to 16 is applied to plant seedlings, seedlings or seeds, and the plants are cultivated. The above control and / or plant growth adjustment method.
23. One or more pest control and / or plant growth selected from weeds, diseases, and pests, characterized in that the agrochemical formulation composition according to any one of claims 1 to 16 is applied to soil for plant cultivation. Adjustment method.
24. The method according to claim 23, wherein 5 to 1000 g of the agricultural chemical preparation composition is applied per 1 m ^{2 of} soil.