WO2017188049A1 - Composition pour formulation de pesticide microbien, son procédé de production et son procédé d'utilisation - Google Patents

Composition pour formulation de pesticide microbien, son procédé de production et son procédé d'utilisation Download PDF

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Publication number
WO2017188049A1
WO2017188049A1 PCT/JP2017/015504 JP2017015504W WO2017188049A1 WO 2017188049 A1 WO2017188049 A1 WO 2017188049A1 JP 2017015504 W JP2017015504 W JP 2017015504W WO 2017188049 A1 WO2017188049 A1 WO 2017188049A1
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acetic acid
genus
formulation composition
agrochemical
composition according
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PCT/JP2017/015504
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English (en)
Japanese (ja)
Inventor
聡信 山崎
大輔 前川
剛一 尾崎
亘俊 明星
壮志 寺田
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クミアイ化学工業株式会社
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Priority to JP2018514509A priority Critical patent/JP6920283B2/ja
Priority to KR1020187033903A priority patent/KR102455374B1/ko
Priority to CN201780026305.1A priority patent/CN109219352B/zh
Publication of WO2017188049A1 publication Critical patent/WO2017188049A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M21/00—Apparatus for the destruction of unwanted vegetation, e.g. weeds
    • A01M21/04—Apparatus for destruction by steam, chemicals, burning, or electricity
    • A01M21/043—Apparatus for destruction by steam, chemicals, burning, or electricity by chemicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M7/00—Special adaptations or arrangements of liquid-spraying apparatus for purposes covered by this subclass
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M9/00—Special adaptations or arrangements of powder-spraying apparatus for purposes covered by this subclass
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds

Definitions

  • the present invention relates to a microbial pesticide preparation composition and the like. More specifically, a fungus having a pesticide, weed, etc. as a pesticide active ingredient that exhibits a control action against pests, weeds, etc., and has improved the control activity of the fungus with acetic acid, and is activated by acetic acid.
  • the highly active microbial pesticide preparation composition that enables long-term survival of the filamentous fungus in a state, that is, excellent in storage stability, its production method, pest and weed control method, and plant growth adjustment method Etc.
  • microbial pesticides utilizing the function of microorganisms have been proposed.
  • the use of these microbial pesticides alone or in combination with synthetic pesticides can reduce the environmental impact, The effect of suppressing the appearance frequency of drug-resistant pests and drug-resistant weeds in question is recognized.
  • Patent Document 1 discloses a disease control agent having a Tallomyces spp. Is a pest control agent or a disease control agent containing a pesticide active as an agrochemical active ingredient
  • Patent Document 3 discloses Verticillium spp.
  • a disease control agent comprising an agrochemical active ingredient is disclosed
  • Patent Document 4 discloses a weed control agent comprising a Drexella species as an agrochemical active ingredient.
  • Patent Document 5 discloses that Trichoderma spp. An example in which the control activity against is improved is disclosed.
  • the present invention is a microbial pesticide preparation composition in which a biopesticide filamentous fungus which is an agrochemical active ingredient is highly activated by acetic acid and can be stored for a long period of time, a method for producing the same, a method for controlling pests and weeds by using the composition, plant growth
  • the purpose is to provide adjustment methods.
  • the present inventors have conducted intensive research, and as a result, biopesticide filamentous fungi using cereal seeds and / or processed seeds (one or more of hulls, straws, and refined products) as a solid medium.
  • the present inventors have found that a microbial pesticide preparation composition containing a solid culture and acetic acid can solve the above problems, and has completed the present invention.
  • the embodiment of the present invention is as follows.
  • Filamentous fungi having a controlling action against pests and / or weeds and a plant growth regulating action include the genus Beauveria, the genus Coniothyrium, the genus Metarhizium, the genus Talaromyces (Talaromyces), Trichoderma
  • the agrochemical formulation according to (3), wherein the filamentous fungus having a controlling action against pests and / or weeds and a plant growth regulating action is Trichoderma sp.
  • the genus Trichoderma is an Asperelloides inoculum, an Asperellum inoculum, an atroviride inoculum, a hamatum inoculum, a harzianum inoculum, or Koningii (Koningii inoculum) ) Agrochemical formulation composition.
  • the agrochemical formulation according to (6), wherein the Aspereroides inoculum is Trichoderma aspereloides SKT-1 strain (FERM BP-16510).
  • Composition. (13) The agrochemical composition according to any one of (1) to (12), wherein the composition is a granular agrochemical formulation, and the solid medium is a granule.
  • the acetic acid is supported on the surface of the biopesticide filamentous fungus solid culture by spraying acetic acid or a solution obtained by diluting acetic acid with water or an organic solvent, as described in (17) or (18) Method.
  • a biochemical pesticide active ingredient which is a pesticide active ingredient, is highly activated by acetic acid and can be stored for a long period of time.
  • Composition a method for producing the composition, a method for controlling pests and weeds by using the composition, a method for controlling plant growth, and the like.
  • the microbial pesticide preparation composition of the present invention is obtained by solid-cultivating agriculturally useful biopesticide filamentous fungi (biological pesticide filamentous fungus solid culture, that is, the cells of cultured biopesticide filamentous fungi (including mycelia and spores). And a mixture of solid medium using grain seeds and / or processed seeds) and acetic acid.
  • biopesticide filamentous fungi biological pesticide filamentous fungus solid culture, that is, the cells of cultured biopesticide filamentous fungi (including mycelia and spores).
  • a mixture of solid medium using grain seeds and / or processed seeds and acetic acid.
  • biopesticide filamentous fungi refers to agricultural production by controlling pests such as pests and weeds, improving the soil to be suitable for cultivation, and promoting or suppressing the growth of the plant itself. It means a filamentous fungus that improves the property, and is not limited to those having a pesticide registration or those clearly showing the effect as a pesticide.
  • Examples include the genus Acremonium, the genus Alternaria, the genus Ambrosiella, the genus Arthrobotrys, the genus Aspergillus, and the genus Aureobasidium Genus, genus Beauveria, genus Blastomyces, genus Botryospolium, genus Botrytis, genus Chalara, genus Cercospora Cephalosporium, Chrysomnia, Chrysosporium (C Lysosporia, Chlorodome, Cladosporium, Coccidioides, Coniothyrium, Cryptocoun, Cryptococcus , Genus Curvularia, genus Cylindrocarpon, genus Cylindrocladium, genus Drechsella, genus Epicoccum, genus Eupenicilium , Fusarium genus, Geotrica (Geotricum), Gliocladium, Graphium, Helicomyces
  • biopesticide filamentous fungus can be used as long as it has a controlling activity against pests and the like, and the present invention is not limited to the above-exemplified fungi, but among others, the genus Boberia, the genus Coniotylium, the genus Metalidium, Preferably, fungi, Trichoderma spp., Verticillium spp. Are used, and Trichoderma spp. Are particularly preferred. Among these Trichoderma spp., Aspereloides spp., Asperellum spp., Atroviride spp., Hamatum spp., Harzianum spp., Koningii spp.
  • Aspereroides spp. Is preferred, and among the Aspereroides spp., The Ministry of International Trade and Industry, Institute of Industrial Science, Biotechnology Institute of Technology, Patent Microorganism Depositary Center (currently the National Institute for Product Evaluation Technology Patent Biological Depositary Center) 1997 The bird that was deposited on November 10th as Trichoderma Atrovilide SKT-1 was transferred to an international deposit on February 13, 2017. Derma-Asupereroidesu SKT-1 strain (FERM BP-16510) is particularly preferred.
  • the number of filamentous fungi (viable bacteria and / or spores) in the agricultural chemical formulation composition of the present invention is not specified, it is usually about 10 to 10 10 10 CFU (Colony Forming Unit) per gram of the agricultural chemical formulation composition, preferably Is about 10 3 to 10 9 CFU, more preferably about 10 5 to 10 8 CFU.
  • the number of filamentous fungi in the agrochemical preparation composition can be measured by a dilution plate method.
  • Examples of the medium used for the dilution plate method include ordinary agar medium, standard agar medium, potato dextrose agar medium, oatmeal agar medium, malt extract agar medium, potato ginseng agar medium, seawater starch agar medium, zapek dox agar medium, zapek yeast
  • Known media such as extract agar medium, MY20 agar medium, Sabouraud / glucose agar medium, Luria / Bertani agar medium, and the like can be mentioned, but are not limited to these examples, and meet the nutritional requirements of the biological pesticide filamentous fungi to be handled
  • the culture medium to be used may be used.
  • agrochemical formulation composition of the present invention one or more selected from cereal seeds, their hulls, straws, and refined products are used for the solid medium of the biopesticide filamentous fungus solid culture.
  • “cereal” is used in a broad sense, and examples thereof include rice, maize, barley, wheat, rye, oat (oat).
  • Any edible seed mainly composed of starch can be used, and the present invention is not limited to the above-exemplified cereals.
  • grass and legume grains are preferable, and barley, among legumes and legume grains,
  • barley among legumes and legume grains,
  • wheat, rice and soybean are particularly preferred.
  • the use of one or more selected from cereal seeds, their hulls, cocoons, and refined products as a solid medium, and the use of a solid culture containing them as a preparation improves the long-term viability of the biopesticide filamentous fungus in the presence of acetic acid. .
  • the cereal seeds may be used as they are, but the outer shell (such as rice husks) produced when the seeds are removed are refined. You may use the koji which comes out, refined itself, and a mixture of these two or more.
  • the seed coat, cocoon, and refined product may be collectively referred to as “processed seed product”.
  • the seed refined product is substantially composed of the endosperm of the seed, and the seed refined product and the endosperm accompanied by an embryo, both of which can be used in the present invention and are not particularly distinguished in the present invention. .
  • Such cereal seeds and processed seeds can be used as they are as a solid medium, and in the present invention, cultivated biopesticide filamentous fungi using cereal seeds and / or processed seeds as a solid medium, The obtained biopesticide filamentous fungus solid culture is used as a raw material for the agrochemical formulation composition together with the medium.
  • Cereal seeds and their refined products are inherently granular, and the seed husks and pods are inherently amorphous. These granular materials or irregular shaped materials can be pulverized into finer particles or powders (granular pulverized products, powdered pulverized products, etc.). The shape can be used as it is.
  • the dosage form of the agrochemical formulation composition of the present invention is naturally determined depending on the shape of the grain seeds and / or processed seed products to be used, and the granular agrochemical formulation composition, the irregular shape agrochemical formulation composition, or the powdered agrochemical formulation composition.
  • it is preferably a granular agrochemical preparation composition, more preferably a granular material having a particle size of 2 mm or more, and therefore, among the seeds and / or processed seeds of the grain, the granular material is preferable.
  • grain seed refined products can be used particularly preferably.
  • the culture method of the biopesticide filamentous fungus according to the present invention can be arbitrarily set according to the type of fungus, strain, and the like. And the seed and / or seed processed material of the said grain are used as a solid medium (carrier of a solid medium) in culture
  • the culture temperature and the culture time can be arbitrarily set, but for example, conditions for culturing at 10 to 40 ° C. for 2 to 30 days are shown. Further, the obtained culture may be appropriately dried.
  • acetic acid is blended for the purpose of activating the activity of the biopesticide filamentous fungus.
  • Acetic acid promotes mycelial growth of biopesticide filamentous fungi and improves the activity as an agrochemical active ingredient, whereby the agrochemical formulation composition of the present invention achieves a high control effect.
  • the amount of the acetic acid is not particularly limited. However, if the amount of acetic acid is too small, a sufficient hyphal growth promoting action cannot be expected. On the other hand, it may act bactericidal.
  • a suitable amount of acetic acid that is expected to have a sufficient hyphal growth-promoting effect on the biopesticide filamentous fungi and that the biopesticide filamentous fungus is not sterilized is usually about 0.01 to 1% by mass based on the total amount of the agrochemical formulation composition. Preferably it is 0.03-0.8 mass%, More preferably, it is 0.05-0.5 mass%.
  • acetic acid may be added to a biopesticide filamentous fungus solid culture and further mixed to homogenize the acetic acid in the powdery pesticide preparation composition.
  • the medium is a granular material, acetic acid may be supported on the surface.
  • a method for supporting acetic acid on the surface of the granular material for example, there may be a method in which acetic acid or a solution obtained by diluting an acid with water or an organic solvent is sprayed on the granular material, but first, hydrous amorphous silicon dioxide (white carbon), etc.
  • a method in which acetic acid is adsorbed on a fine powder of mineral and then the acetic acid adsorbate is coated on the surface of a solid medium is preferable. In this case, the long-term viability of the biopesticide filamentous fungus in the presence of acetic acid is further improved.
  • the amount of the fine mineral powder loaded with acetic acid is not particularly limited, but is usually about 0.2 to 3% by mass based on the total amount of the agricultural chemical formulation composition.
  • the content is preferably 0.5 to 2% by mass, more preferably 0.8 to 1.5% by mass.
  • a biopesticide is obtained by solid-culturing a biopesticide filamentous fungus using one or more selected from cereal seeds, its hulls, cocoons, and refined products as a medium. After obtaining a biopesticide filamentous fungus solid culture in which the filamentous fungus adheres to the solid medium, a production method of adding acetic acid to the culture can be shown. As an example of a more preferable production method, a biopesticide filamentous fungus is solid-cultured using a solid medium that is a grain seed or a refined product of grains, and the cultured biopesticide filamentous fungus adheres to the granular solid medium.
  • a production method for supporting acetic acid on the surface of the culture can be shown.
  • a biopesticide filamentous fungus is solid-cultured using a solid medium that is a grain seed or a refined product of a grain seed, and the cultured biopesticide filamentous fungus adheres to the granular solid medium.
  • the production method of coating the surface of the culture with water-containing amorphous silicon dioxide adsorbed with acetic acid after obtaining the solid culture of the biopesticide filamentous fungus to be performed can be shown.
  • the application method of the agrochemical formulation of the present invention can be appropriately selected depending on the type of plant, type of pest, application location, application time, dosage form, and the like.
  • the agrochemical formulation composition of the present invention can be applied directly as it is, or diluted with water or a carrier and applied.
  • Application methods include spraying on plant stems and leaves, spraying on plant stocks, spraying on the surface of the soil, soil mixing, soil irrigation, water surface application, seed dressing, application, soaking method, etc. It is not limited to.
  • the agrochemical formulation composition of the present invention can be mixed with other fungicides, insecticides, nematicides, herbicides, plant growth regulators, fertilizers, soil improvement materials, etc. as necessary, applied alternately, or simultaneously. It can also be applied, and in this case, a more excellent effect may be exhibited.
  • the application place of the agrochemical formulation composition of the present invention can be applied to nurseries, field fields, paddy fields, orchards, hydroponic culture facilities, etc. for cultivating agricultural and horticultural plants, but is not limited thereto.
  • the application time of the agrochemical formulation of the present invention is not limited during the planting period. In the case of the main field, before planting, at the time of planting, after planting, in the seedling period, any time before sowing, simultaneously with sowing, or after sowing But it can be applied.
  • the application rate of the agrochemical formulation composition of the present invention varies depending on the type of applied plant, the type of pests and weeds, the condition of the soil, the application time, the planting density, the dosage form, etc. In this case, about 100 to 1000 g per 1 m 2 of soil can be used, and in the case of main field, about 5 to 1000 g per 1 m 2 of soil can be used.
  • the pulverized product can be used directly or diluted with water and used at about 1 to 100 g per 1 kg of seeds. .
  • plants to be applied with the agrochemical formulation composition of the present invention include cereals (eg, rice, wheat, barley, rye, oat, corn, sorghum, millet, millet, barnyard millet, millet, buckwheat).
  • cereals eg, rice, wheat, barley, rye, oat, corn, sorghum, millet, millet, barnyard millet, millet, buckwheat.
  • Potatoes eg, potato, sweet potato, taro, yam, konjac
  • beans eg, soybean, azuki bean, kidney bean, pea, broad bean, groundnut, cowpea, chickpea, yellow bean
  • vegetables eg, eggplant, tomato, Peppers, peppers, cucumbers, melons, watermelons, pumpkins, zucchini, shiroi, yugao, tougan, bitter gourd, cabbage, Chinese cabbage, broccoli, cauliflower, radish, turnips, gentian rhinoceros, komatsuna, mizuna, leek, onion, leek, garlic, rakki US Lagas, lettuce, burdock, garlic, buffalo, carrot, honeybee, celery, parsley, strawberry, spinach, okra, perilla, basil, mint, ginger, ginger, fruit tree (eg, apple, pear, pear, quince, quince) , Cherry, peach, plum, ume, apricot, chestnut, walnut, almond, pecan
  • the agrochemical formulation composition of the present invention has excellent pest control activity and is useful as a fungicide, nematicide, insecticide, herbicide, plant growth regulator or soil conditioner.
  • the effects as fungicides, nematicides, and insecticides are not limited to those directly showing control effects against pathogenic bacteria, nematodes, and pests.
  • fungi, nematodes, insects that are mediators It also includes the control of viral diseases by controlling the above, and the indirect control of suppressing the growth of pests due to the predominance of the biopesticide filamentous fungi in the soil.
  • pathogenic bacteria examples include filamentous fungi, actinomycetes, bacteria, viruses, viroids, and the like.
  • the genus Ustylago for example, Barley bare smut fungus (Ustilago nuda), the genus Tilletia (eg, Tilletia caries), the genus Puccinia scab (eg, fungus) (Puccinia recondita), Gymnosporangium spp., For example Gymnosporadium asiaticum, Phacosoporico spp., For example soybean rust Asparagus purple crest fungus (Helicobasidium mom) a), genus Exobasidium, such as Chamochi fungus (Exobasidium vexans), genus Rhizoctonia, such as Rhizoctonia solani, sclerotium Cucumber and soybean white
  • genus Botrytis such as cucumber gray mold fungus (Botrytis cinerea), genus Cercospora, such as sugar beet fungus (Cercospora beticola), Pseudosor spo Pseudocercospora vitis, Paracercospora genus, such as eggplant brown rot (Paracercospora Vietnamesea), Cercosporella genus, such as cercosporacosporacer )
  • Genus fungi such as wheat eye spot disease fungus (Pseudocercosporella herpotriochoides), Corynespora spp., Eg cucumber brown spot fungus (Corynespora casicola), Mycoberosiella spp.
  • Mycovellosiella natrassi Mycovellosiella natrassi, genus Passalora, for example, Tomato leaf mold (Passalora fulva), genus Curvularia, for example rice brown rice (Curvularia spp. ), Fusarium spp., For example, Fusarium oxysporum f. Sp. Lycopersici, Penicillium spp., For example, Penicillium digitatum, Pyricularia, Pirularia spp.
  • Tomato leaf mold Passalora fulva
  • genus Curvularia for example rice brown rice (Curvularia spp. )
  • Fusarium spp. For example, Fusarium oxysporum f. Sp. Lycopersici
  • Penicillium spp. For example, Penicillium digitatum, Pyricularia, Pirularia spp.
  • Verticillium spp. Such as eggplant half-wilt fungus (Verticillium dahlia), Taphrina spp., Peach currant fungus (Taphrina deformans), erum Powdery mildew (Blumeria graminis f. Sp. Tr tici), Erysiphe genus bacteria such as grape powdery mildew (Erysiphe necator), Sphaerotheca genus bacteria such as strawberry powdery mildew (Sphaerotheca aphanis), Podosphaera (Podosphaera) xanthii), Leveillula spp.
  • Verticillium spp. Such as eggplant half-wilt fungus (Verticillium dahlia), Taphrina spp., Peach currant fungus (Taphrina deformans), erum Powdery mildew (Blumeria graminis f. Sp. Tr
  • Phytophthora spp. For example, Phytophthora sojae, Pythium spp., For example, Phythium spp., Albgo spp. Bacteria (Albgo macrospora), Peronospora (eg Peronospora destructor), Bremia (eg, Bremia lactoporodeporporus), Pseudospora peus For example, Cucumber downy mildew (Pseudoperonospora cubensis), Plasmopara (Plasmopara) genus E.g., Plasmopara viticola, Aphanomyces, e.g., Aphanomyces cochlioides, e.g.
  • Plasmodiophora e.g., Plasmophora Spongospora spp.
  • Plasmodiophora e.g., Plasmophora Spongospora spp.
  • Plasmophora Spongospora spp. For example potato powder scab, Streptomyces spp., Streptomyces spp., Clavibacter tomato, Clavibacter spp.
  • Clavibacter mi higanensis Pectobacterium, such as potato soft rot, Rhizobium, such as Rhizobium ratobacter, Rhizobium ratobacter Ralstonia solanacerum, Burkholderia genus bacteria, for example, Burkholderia glumae, Acidoborax genus bacteria, for example, Rice brown moss ) Genus, for example lettuce rot fungus Pseudomonas spp.
  • Pectobacterium such as potato soft rot
  • Rhizobium such as Rhizobium ratobacter, Rhizobium ratobacter Ralstonia solanacerum
  • Burkholderia genus bacteria for example, Burkholderia glumae
  • Acidoborax genus bacteria for example, Rice brown moss
  • Genus for example lettuce rot fungus Pseudomonas spp.
  • Xanthomonas spp. Such as cabbage black rot fungus (Xanthomonas campestris), Frovirus (Furovirus), eg, wheat dwarf virus (SBWMV), Tobamovirus (Tobamovirus), Tobravirus genus such as tobacco stem virus (TRV), Potexvirus genus such as potato X virus (PVX), Carlavirus genus such as carnation latent virus (CaLV), Clini Virus genus, for example, cucurbit yellowing virus (CCYV), genus Bymovirus, eg For example, wheat stripe dwarf virus (WYMV), Potyvirus, eg Potato virus Y (PVY), Alfavirus, eg Alfalfa mosaic virus (AMV), Cucumovirus, eg Cucumber Mosaic virus (CMV), Comovirus genus such as radish mosaic virus (RaMV), Fabavirus genus such as broad bean virus (BBWV), Nepovirus
  • pathogenic bacteria in particular, Helicobacterium, Rhizoctonia, Skrerotium, Homopsis, Pyrenoceta, Choletotricum, Fusarium, Verticillium, Caronectinia, Gomanomyces spp., Roselinia spp., Monosporacus spp., Didimela spp., Sclerotinia spp., Rhizopus spp., Phytophthra spp., Aphanomyces spp., Plasmodiophora spp.
  • Genus Clavibacter, Pectobacterium, Rhizobium, Ralstonia, Frovirus, Tobamovirus, Tobravirus, Vimovirus, Nepovirus, Carmovirus, Offiovirus, etc. Suitable for soil-borne pathogens according to the present invention It can control.
  • the genus Apherenchoides for example, Aphlenchodes besseyi, the genus Bursaphelenchus, For example, pinewood nematode (Bursaphelenchus xylophilus), genus Dityrenchus (for example, Imogusaresenchu (destructor), genus globodera, for example potato strode He erodera glycines, genus Meloidogyne, such as sweet potato root nematode (Meloidogyne incognita), genus Pratylenchus, such as Pratylenchus genus, , Genus Tylenchus, for example, Tylenchus semipenetran, and the like, but the present invention is not limited to these examples.
  • grasshopper pests such as the grasshopper (Rusporia lineosa), the cricket family Telegrillus emma, and the vignetting family (Gryllotalpa orientalis), Kobaneinago of acrididae (Oxya yezoensis), migratory locust (Locusta migratoria), My Guratori over grasshoppers (Melanoplus sanguinipes), piggyback grasshopper (Atractomorpha lata) of the piggyback grasshopper family, of pine cricket family Kayakoorogi (Euscyrtus japonicus), of Nomibatta Department Flea grasshopper (Xya japonicus), thrips Eye pests, for example of the thrips family Hirazuhanaazamiuma (Frankliniella intonsa), western flower thrips (Frankliniella occidentalis), yellow tea thrips
  • tarsa decemlineata western corn root worm (Diabrotica virgifera), cucumber moth beetle (Alacophora femorarias), radish beetle (Phaedon brasiumae), physalis weevil (Phylotletolata) Weevil (Hypera postica), Weevil weevil (Listroderes costirostris), Weevil weevil (Euscepes postfascituus), Weevil weevil (Echinocnemus bipunctas), Weevil weevil rhoptrus oryzophylus), weevil of the family weevil (Sitophilus zeamis), shirasoue sushi (Sphenophrus venasis), pine stag beetle (Tomicus pinipacidae), Bark beetle (Lyctus bruneus), fly insect pests, for example, Tipula aino
  • Spectabilis Malacosoma nestrium testaceum, Spruce family shrimp (Agrius convulvali), Arnapseconspersa, Arnapanthiconspersa, Mytah ipsilon, Tamanaginu waiba (Autographa nigrisigna), Helicoverpa armigera, Corn ear worm (Helicoverpa zea), Tobacco budworm (Heliothis virescens d) exigua), Spodoptera litura, bee pests, for example, Arge pagana, Bee family, Aphemus muri, Athaerus Vespa similima xanthoptera, ants of the antaceae (Solenopsis invicta); For example, Lepisma saccharina, Yamatosimi (Ctenolepisma villosa), Cockroachate pests, for example, Cockroachaceae, B.
  • Cannibal termites Incitermes minor
  • Cyprids termites Copttermes formosanus
  • Termites Donopterformes formosanus
  • Pterodoptera Cicadas Liposcelis corrodens, white-tailed pests, for example, Chrysophyllum dipteridae (Lipeurus caponis), Belgian white-headed lice (Demilinia bovis), L (Pediculus humanus), Canine lice (Linognathus setosus), Phyrus pedis (Pthyrus pubis), plant mites (Penthalus majoli) ( olyphagotarsonemus latus), a kind of Shiramidani of Shiramidani Department (Siteroptes sp.
  • red cloveraceae weeds such as Oenothera erythrosepala, Oenothera laciniata, buttercup weeds such as Togenino fox ranunculus Ranunculus sardous, terrestrial weeds such as buckwheat (Polygonum convolvus), sanaetadegi (Polygonum raspi forum), Polygonum sap (um) Shi (Rumex obtusifolius), Japanese knotweed (Poligonum cuspidatum), Pennsylvania smart weed (Polygonum pensylvanicum), knotweed (Persicaria longiseta), Ooinutade (Persicaria lapathifolia), Tanisoba (persicaria nepalensis), Portulacaceae weeds such purslane (Portulaca oleracea), dianthus Family weeds such as chickweed (Stellaria
  • Physalis angula (Physalis angulata), American dogwood (Solanum americanum), Barnacle beetle (Solanum carolinense), scorpionaceae weeds, for example, Veronica persica (Vernica persica), Sabasou (Veronica hederaefolia, Asteraceae weeds, for example Eclipta prostrata (Eclipta prostrata), Bidens Tripartita (Bidens tripartita), cocklebur (Xanthium pensylvanicum), wild sunflower (Helianthus annuus), chamomile (Matricaria chamomilla), Inukamitsure (Matricaria perforataorinodora), corn marigold (Chrysanthhemum segetum), Koshikagiku (Matricaria matricarioides), Ragweed (Ambrosia artemisifolia), Obraxa trifida (Ambrosia trifida) igeron canaden
  • the agricultural chemical formulation composition of the present invention for example, land for industrial facilities such as bank slopes, riverbeds, road shoulders and slopes, railway floors, park green spaces, grounds, parking lots, airports, factories and storage facilities, It can weed a wide range of weeds that occur in non-agricultural lands where it is necessary to control the growth of weeds, such as fallow land, or closed land in the city, or in orchards, pastures, lawns, and forestry areas.
  • the pesticide preparation composition of the present invention can also be used as a target for control in rivers, waterways, canals, reservoirs, and the like, for example, Shara spiders (Chara braunii), Duckweeds such as Spirodella polyrhiza, Thais such as Ginkgo biloba. (Ricciocarpus natans), Hosimidroaceae, for example, Spirogyra arcura, Mizuaoii, for example, Eichhornia crassipes, Salamander, for example, Azolla sect.
  • button duckweed Pistia s Ratiotes
  • haloragaceae eg Myriophyllum aquaticum (Myriophyllum aquaticum) and the like
  • Myriophyllum aquaticum Myriophyllum aquaticum
  • the “biopesticide filamentous fungus solid culture” and the “acetic acid adsorbate” both specify the structure simply by indicating the state, and the product is specified by the manufacturing method. Absent. In addition, it is excessively expensive and time-consuming to perform the work and analysis for specifying the structure of these objects other than those described above.
  • Trichoderma harzianum T-22 strain is sold as a soil conditioner under the trade name of Trichodesoil (registered trademark, manufactured by Arista Life Science Co., Ltd.).
  • Trichoderma hamatum NBRC 31932 shares were sold by National Institute of Technology and Evaluation.
  • Talalomyces flavus SAY-Y-94-01 is marketed as an agrochemical under the trade name Tough Block (registered trademark, manufactured by Idemitsu Kosan Co., Ltd.).
  • Metalidium anisoprie SMZ-2000 strain is marketed as an agrochemical under the trade name of Pirate Granules (registered trademark, manufactured by Arista Life Science Co., Ltd.).
  • Trichoderma spp. Spores can no longer be observed at an acetic acid concentration of about 0.15 to 0.3% by mass. In other words, even if spores are grown, it is difficult to grow by simply mixing acetic acid above a certain concentration. It was shown that. In addition, the spores of Talalomyces flavus SAY-Y-94-01 and Metalidium anisoprie SMZ-2000 have almost the same results as Trichoderma sp., Although there are some differences in mycelial growth at each acetic acid concentration. It was.
  • the acetic acid bulk powder coating preparation was prepared by adsorbing acetic acid to white carbon to give 15% by weight of bulk powder, 8 g of SKT-1 strain barley seed medium culture (barley seed (variety: Hayadri 2)), YG medium (0.3 mass) 0.5 ml of SKT-1 strain spore suspension (1 ⁇ 10 6 spores / ml) was inoculated into a 100 ml Erlenmeyer flask containing 8 ml of 2% yeast extract and 2.0% by weight glucose, and allowed to stand at 25 ° C. for 8 days.
  • Acetic acid was adsorbed by spraying the solution while shaking the solid culture. After preparation, the preparation was packed in an aluminum bag and stored at 5 ° C. After 0 days (initial value), 1 day and 7 days later, the viable cell count in the preparation was measured by a dilution plate method. The test results are shown in Table 2.
  • the filamentous fungus solid-cultured preparation and the acetic acid direct-injection preparation of the filamentous fungus solid culture according to the present invention survive to a certain extent even after 7 days of manufacture, whereas the filamentous fungus solution simply contains acetic acid.
  • the number of viable bacteria immediately after production was smaller than that of the product of the present invention, and the survival of the filamentous fungus was not already observed one day after production.
  • Contaminated soil was obtained by mixing well at a ratio and leaving it in a moist chamber (27 ° C., dark place) overnight. About 15 ml of this contaminated soil was inoculated by spreading on the stock of a cotyledon stage cucumber (variety: Nissho) that was raised in a plastic cup (diameter 6 cm). After inoculation, the acetic acid bulk powder coating preparation and direct acetic acid injection preparation of Example 2 and SKT-1 strain solid culture were sprayed at 500 g / m 2 equivalent to the cucumber stock, and the SKT-1 stock solution was the SKT-1 strain.
  • Control value (1-disease rate in treated area / disease rate in untreated area) x 100
  • the acetic acid bulk powder coating preparation and the acetic acid direct-injection preparation of the product of the present invention exhibit the same degree of control effect as that of Torcrolofosmethyl wettable powder, which is an organophosphorus fungicide, against cucumber silkworm, and It was revealed that the control effect was higher than that of the SKT-1 strain solid culture or the SKT-1 strain solution.
  • Cucumber seedling blight fungus (Rhizoctonia spp.) Of agricultural chemical formulation composition) was pre-cultured on a PDA plate medium, and the agar-containing agar pieces were mixed with soil bran medium and cultured at 27 ° C. for 7 days.
  • a square bowl (9 cm square) was filled with 200 ml of sterile river sand and 100 ml of contaminated soil in this order.
  • Soil incorporation of acetate bulk powder coating formulation of Example 2 (acetate amount 0.15%) is filled before, contaminated soil 100ml and acetic bulk powder coating formulation 8.1 g (1000 g / m 2 corresponds to the contaminated soil to the corner pots ) was mixed in a plastic bag, filled into a square bowl, watered, and then sown with cucumber (variety: Nissho).
  • the surface layer spraying of the acetic acid bulk powder coating preparation was carried out by spraying cucumber seeds on the contaminated soil after irrigation and then uniformly spraying 500 g / m 2 equivalent on the soil surface layer.
  • Disease severity ⁇ (number of diseased seedlings by index ⁇ index) / (number of surveyed seedlings ⁇ 2) ⁇ 100
  • the powdered acetic acid powder preparation of the present invention exhibits the same level of control effect as that of tolcrofosmethyl wettable powder in both soil admixture treatment and soil surface treatment for cucumber seedling blight (Rhizoctonia). Became clear.
  • a cucumber seedling fungus (Pythium aphanidermatum) was pre-cultured on a PDA plate medium, and the agar-containing agar pieces were mixed with a soil bran medium and cultured at 27 ° C. for 7 days.
  • a square bowl (9 cm square) was filled with 200 ml of sterile river sand and 100 ml of contaminated soil in this order.
  • the soil mixing of the acetic acid bulk powder coating formulation of Example 2 (acetic acid content 0.15% by mass) was performed before filling the contaminated soil into a bowl, 100 ml of contaminated soil and 4.1 g of acetic acid bulk powder coating formulation (500 g / m 2).
  • the mixture was mixed in a plastic bag, filled into a square bowl, watered, and then sown with cucumber (variety: Nissho).
  • Disease severity ⁇ (number of diseased seedlings by index ⁇ index) / (number of surveyed seedlings ⁇ 2) ⁇ 100
  • the SKT-1 strain solid culture obtained by using various grains as a solid medium and the preparation containing acetic acid all show the same or better control effect against cucumber silkworm against Torquelofosmethyl wettable powder. Became clear.
  • the present invention is a microbial pesticide formulation composition in which a biopesticide filamentous fungus, which is a pesticide active ingredient having a control action against pests and / or weeds and a plant growth regulating action, is highly activated with acetic acid, and can be stored for a long period of time.
  • An object of the present invention is to provide a microbial pesticide preparation composition, a method for producing the same, a method for controlling pests and weeds by using the composition, a method for controlling plant growth, and the like.
  • biopesticide filamentous fungus solid culture in which one or more selected from cereal seeds, their hulls, cocoons, and refined products are used as a solid medium, and an agrochemical formulation composition containing acetic acid, To solve.

Abstract

[Problème] L'invention a pour but de fournir : une composition pour une formulation de pesticide microbien dans laquelle un champignon filamenteux pesticide microbien, qui est un ingrédient actif pesticide ayant un effet de lutte contre les parasites et/ou les mauvaises herbes, et de régulation de la croissance des plantes, est hautement activé par l'acide acétique, ladite composition pour formulation de pesticide microbien étant apte à être stockée pendant de longues périodes; un procédé de production de ladite composition; un procédé de lutte contre les parasites et les mauvaises herbes à l'aide de cellle-ci; et un procédé de régulation de la croissance des plantes. [Solution] Le problème mentionné ci-dessus est résolu à l'aide d'une composition pour formulation de pesticide contenant : un milieu de culture solide filamenteux de pesticide microbien ayant un ou plusieurs éléments sélectionnés parmi des graines de céréales, les enveloppes, le son ou les graines broyées de celles-ci en tant que milieu de culture solide; et de l'acide acétique.
PCT/JP2017/015504 2016-04-28 2017-04-17 Composition pour formulation de pesticide microbien, son procédé de production et son procédé d'utilisation WO2017188049A1 (fr)

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CN112646734A (zh) * 2020-12-31 2021-04-13 广西壮族自治区林业科学研究院 一株兰花菌根真菌pf06及其应用

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KR102193709B1 (ko) 2019-01-25 2020-12-21 인바이오 주식회사 과립수화제형 농약 조성물 및 그의 제조방법
CN113180065B (zh) * 2021-05-27 2022-06-24 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) 一种苦荞芽苗提取物的应用
CN114872157B (zh) * 2022-06-07 2023-05-09 福建省林业科学研究院 一种新枯死松材线虫病疫木树干的处理方法

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