JPH06277042A - New strain of genus drechslera, and weed-controlling agent and weed-controlling composition containing same - Google Patents

Abstract

PURPOSE:To provide the subject strain not exhibiting pathogenicity on cultivated plants, excellent in selectivity, safety and environmental pollution resistance, and useful for weed-controlling agents, etc., among pathogenic microorganisms, Drechslera monoceras, exhibiting pathogenicity on the genus Echinochloa spp. plants at <= a prescribed temperature. CONSTITUTION:This microorganism is a new strain, MH 111012 (FERM- BP-3865), not exhibiting pathogenicity on cultivated plants such as rice among pathogenic microorganisms, Drechslera monoceras, exhibiting the pathogenicity on Echinocloa spp. at <=15 deg.C. A weed-controlling composition is obtained from the strain and one or more kinds of chemical herbicides such as a diphenyl ether herbicide.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a novel microorganism, a weed control agent and a weed control composition containing the same.

[0002]

2. Description of the Related Art Recently, there has been a serious problem such as environmental pollution due to excessive use of organic synthetic pesticides and reduction of control effect due to emergence of pests and weeds having acquired drug resistance. In the mass media as well, the impact of chemical pesticides on the human body and natural environment has been widely taken up, interest in biopesticides has increased, and research and development of biopesticides have become active. However, pesticide-free cultivation methods and biological control methods are
It is still in the middle of research, and it is a fact that its application alone does not provide a stable control effect, and sufficient crop production cannot be expected. In any case, reducing the application amount of the synthetic organic pesticide is very significant from the viewpoint of safety. In the field of herbicides, research and development of microbial herbicides utilizing pathogenic microorganisms for weeds has advanced mainly in the United States,
Until now, we have used the potato weed, Stranglewein (Morrenia odorata) pathogenic microorganism Phytophthora genus Palmibola species (Phytophthora palmivola) using Devine (DeVine, Abbott brand name),
Leguminous weeds, Northern Joint Vetch (Aeschynome
ne virginica), a pathogenic microorganism of the genus Colletotrichum gloeosporioides
Collego (trade name of Ecolegen Co., Ltd.), etc., which is used, is on the market. However, no microbial herbicide has been put to practical use for the main genus Echinochloa, which is a major weed in the cultivation of major crops such as wheat, rice and soybean. Weeds of the genus Musca germinate and grow in early spring in Japanese paddy fields when the water temperature exceeds 10 ° C. In Japan, the control time of the genus Echinochloa is from May to June, and the water temperature of the paddy field at this time is generally 10 to 15 ° C. On the other hand, the filamentous fungi that have been found to be pathogenic for Nobies do not grow hyphae unless the temperature is 15 ° C or higher. Therefore, even if the filamentous fungus is treated at the time of controlling Nobie, a sufficient controlling effect cannot be expected. The wild species of Nobie are known as weeds in the rice-growing regions of the world, and have long been a problem as a typical paddy weed in Japan. It is said that the outbreak of fly caused the rice planting work in paddy fields. According to Yabuno (Weeds Study (1975) vol.20), wild plants that are wild in the world are Echinochloa oryzicola Tainubie, Echinochloa corona, Echinochloa pyramidalis, and Echinochloa pyramidalis. Echinochloa stagnina), Echinochloa haploclad
a) and the Echinochloa cru species
s-galli), and there are three varieties of Crusaderae species: Formocensis (Himetainubier), Kurusgarii (Innervier), Platycola (Himeinubier).
It is classified into. Responses to herbicides and pathogens differ between such varieties. The naturally occurring weed Novie is considered to be a hybrid of various species of the genus Echinochloa, and as a herbicide having a practical control effect, it must have a herbicidal action on all species of the genus Echinochloa. In paddy fields and upland fields, many weeds compete with crops in addition to Nobie, and the control of these weeds is also essential in practical situations. At present, it is common to mix several chemical herbicides to control many of these weeds at one time.
There is no known composition in which a microorganism having pathogenicity to the genus Mussel and a chemical herbicide are mixed at 5 ° C or lower. This is probably because the microorganisms are generally considered to have no resistance to pesticides including herbicides.

[0003]

DISCLOSURE OF THE INVENTION The present invention solves the above disadvantages in crop production, avoids environmental pollution and the emergence of resistant weeds, and enables practical weed control. An agent and a weed control composition are provided. That is, strains of the genus Dorexella that are pathogenic for only Nobie are infected with all species of the genus Echinochloa even at 15 ° C or lower, and a strain having herbicidal activity is isolated from the natural world and contains live cells thereof. An object is to provide a weed control agent. Furthermore, it shows pathogenicity only to Novie, 15
It is an object of the present invention to provide a safer and sufficiently practical weed control composition by reducing the application rate of a chemical herbicide by using a new strain of the genus Dreyxella that is infected even at a temperature of ℃ or below and a chemical herbicide in combination. .

[0004]

[Means for Solving the Problems] The present inventors have searched various pathogenic microorganisms for the purpose of controlling important weeds such as paddy fields and upland fields. We have found a novel bacterium of the genus Drexella that exhibits herbicidal activity and is not pathogenic to useful plants such as rice. In addition, by mixing the new variant of the present invention of the genus Dorexella which shows selective pathogenicity to Nobie and an existing chemical herbicide, a remarkable synergistic effect is exhibited, which is 1/10 to 1/100 of the conventional one. It was found that a chemical herbicide having an application rate of 10 can exert a sufficient controlling effect on Nobie. As for the research on pathogenicity to the genus Mussel, which has been published in patents, literature, etc., "On Diseases of Tainubie Population" by Okayama University Institute of Resource Biology, Nishi et al.
(Summary of Weed Research, 27th Lecture Lecture, Vol. 33, another issue,
163), "About the disease and insect damage of Taenia cinerea"
(Weed Research, Abstracts of the 31st Lecture, 202 pages) and Paola Del Serrone et al., Institute of Vegetable Pathology, Italy, "Pathogenicity and host range tests of Exse.
rohilum sp.isolated from barnyardgrass '', Phytop
arasitica 19,3 1991 and the inventors of the present invention, "Weed control agents containing a bacterium of the genus Dreyxella or its metabolites and weed control methods using them" (Japanese Patent Laid-Open No. Hei 3-21).
No. 9883), "Weed control composition containing a bacterium of the genus Dreyxella and a chemical herbicide" (Japanese Patent Laid-Open No. 4-226).
905), "New strains of Drechslera spp., And
weed control agents and weed control composition ''
(European PatentApplication 0 464 416 A2, 1992),
"Study on Environmentally Friendly Bioherbicides-First Report-" (199
2nd Annual Meeting of the Japanese Society of Agricultural Chemistry, 569 pages,
"Filamentous Fungus Isolated from Paddy Field Weeds, and the Herbicidal Activity", (Journal of the Japanese Society for Plant Pathology, October 1992, V
ol. 58, No. You can find eight references (page 9, 581). Nishi et al. "About disease of Tainubie population"
(Summary of Weed Research, 27th Lecture Lecture, Vol. 33, another issue,
163), "About the disease and insect damage of Taenia cinerea"
The filamentous fungus used in (Weeds Research, Abstracts of the 31st Lecture, p. 202) is clearly different from the bacterium of the genus Leptosphaeria, which is a genus of the genus Leptosphaeria, used by the present inventors. In addition, Paola Del Serrone et al.
y and host rangetests of Exserohilum sp. isolated
The strain used in "from barnyardgrass, Phytoparasitica 19,3, 1991" is the genus Drechslera sp. used by the present inventors according to the Eris taxonomy, but it has not been identified. The test conditions are 28 ° C., and there is no description about infection at low temperature. Thus, even when looking at the past reports, the strain of the genus Dorexella which is infected at 15 ° C or lower has not been known yet. Also,
As for the strain of the present invention, the inventors of the present invention have disclosed the patent publication, Hei 3-219.
Unlike the conventional strains dealt with in No. 883 and No. 4-226905, strains related to the present invention, even in the strain of the genus Dolerxella monoceras, have the property of infecting Nobies even at a low temperature of 15 ° C. or lower.

The Nobye which is the subject of the present invention is a weed belonging to the genus Echinochloa of the family Gramineae, and more specifically, it is an Echinochloa oryzicola or a Japanese hemlock fly (Ec).
hinochloa crus-galli var. formosensis), barnyard grass (Echinichloa crus-galli var. crus-galli), barnyard grass (Echinochloa crus-galli var. praticola),
Echinochloa colona, Echinochloa pyramidalis, Echi
Nochloa stagnina, Echinochloa haploclada, etc.
That is, the novel microorganism according to the present invention, cultivated plants, rice, barley, wheat, rye, oats, corn, sorghum, millet and grass, orchardgrass,
It does not show any pathogenicity to Italian ryegrass, perennial ryegrass, sweet vernalgrass, tall fescue, medofesque, medfescue, etc., and it is pathogenic to Echinochloa oridicola, Echinocloa crusgalli, Echinocloa corona even at low temperatures of 15 ° C or lower. Is a new strain of Drechslera monoceras (Drechslera monoceras). Pathogenic microorganisms were purely isolated from naturally-occurring pathogens, and as a result of testing the pathogenicity against pathogens and rice, they showed strong pathogenicity against all species of the genus Echinochloa, which are weeds, and against rice. Those showing no pathogenicity were selected. The selected microorganism was identified based on the morphology of conidia, and as a result, it was identified as a new strain of the genus Drexlera (Drechslera monoceras), and the present invention was completed.

The method of using the microorganism of the present invention as a weed control agent may be a method of directly using the viable cells obtained by culturing, a method of using the filtrate after culturing the cells, or a mixture of both. There is a method to use. Further, the microorganism according to the present invention is a herbicide which does not show antagonisticity when the conidia or mycelium obtained by culturing in a nutrient medium are suspended in an aqueous solution containing a surfactant or the like and the microorganism is used alone or when it is not antagonistic. It may be used as a mixture with chemical pesticides such as bactericides and insecticides. When the microorganism according to the present invention is used as a weed control agent, conidia having higher durability than hyphae are more desirable. The bacterial cell growth of the microorganism of the present invention can be performed using either a liquid medium or a solid medium.
Inoculate in a liquid medium such as dextrose to grow the cells,
Conidia are formed by crushing the obtained bacterial cells and then drying. In the case of a solid medium, conidia formation is promoted by inoculating a potato-dextrose agar medium or the like, removing the growing aerial hyphae and then drying. As described above, the microorganism according to the present invention is capable of large-scale culture of mycelia such as conidia and hyphae, and can be industrially used as a weed control agent, and the weed control agent can be used, for example, in paddy fields. It shows herbicidal activity only to the weeds Nobye when treated at the time of transplanting rice to rice, cultivated plants, rice, barley, wheat, rye, oats,
Maize, sorghum, millet and other grasses, orchardgrass, Italian ryegrass, perennial ryegrass, sweet vernalgrass, tall fescue, medfefescue, medfescue and the like found to have a selective herbicidal effect against the present invention, the present invention Was completed.

The novel varieties according to the present invention do not have any biological effect on some chemical herbicides such as mycelial elongation inhibition and spore germination inhibition, and when mixed, the herbicidal action against Nobie is dramatically improved. It was found to improve. As a result of further studies on various herbicides, a novel microorganism according to the present invention, a chemical herbicide of the genus Dolexrella genus, oxadiazone, dimetamethrin, cimetrin, clomethoxynil, dimepiperate, trifluralin, naproanilide, paraquat, pyrazoxifene,
Pyrazolate, butachlor, pretilachlor, bench ocarb, mefenacet, molinate, CNP, D
BN, MCP, promethrin, benzophenap, propanil, NSK-850, HW-52, cromeprop, esprocarb, bifenox, quinclorac, bromobutide, bensulfuron-methyl, pyrazosulfuron-ethyl, 2,4D and the like were mixed. The weed-controlling composition has enabled the control of Nobie by an application rate which cannot be controlled by a conventional chemical herbicide alone, thus completing the present invention. Regarding its mechanism of action, the absorption and migration of chemical herbicides are enhanced due to the invasion marks in plant tissues caused by infection with Drexlera spp. Similarly, in plant tissues damaged by chemical herbicides, It is presumed that the infection of bacteria became easier. Reducing the application rate of chemical herbicides through the synergistic action of these Drexella spp. And chemical herbicides improves recent problems such as environmental pollution and the emergence of resistance, and not only agricultural producers but also general consumers. There is a big advantage for me. In the present invention, CNP, chloromethoxynil, bifenox, mefenacet, pretilachlor, naproanilide, butachlor, propanil, clomeprop, oxadiazone, pyrazolate, bensulfuron-methyl, pyrazosulfuron-ethyl, benchocarb, dimepiperate, molinate, esprocarb, cimetrin, etc. Chemical herbicides are compounds which are already commercially available or are known.

To use the weed-controlling agent and the weed-controlling composition according to the present invention as a herbicide, the cells of a bacterium of the genus Dolexlera and the raw material of a chemical herbicide may be used as they are, but generally, they are generally used as a herbicide. By mixing the bacterial cells of the genus fungus and the active ingredient of the chemical herbicide with an inert solid carrier or liquid carrier, and preparing into a commonly used formulation form such as granules, flowables, wettable powders, emulsions, liquids, etc. It is desirable to use. As the carrier, usually used in agricultural and horticultural agents,
Any biologically inert solid or liquid can be used, and it is not limited to a specific one. For example, as a solid carrier, clay, talc, bentonite, zeolite, calcium carbonate, diatomaceous earth,
Mineral powders such as white carbon, soybean powder, vegetable powders such as starch, and polymer compounds such as polyvinyl alcohol and polyalkylene glycol are listed. Examples of the liquid carrier include various organic solvents such as decane and dodecane, vegetable oil, mineral oil, water and the like. The weed control agent according to the present invention has a content of the bacterium of the genus Drexella is 10 ² to 10 ¹⁵ spores / g, preferably 1
It is 0 ⁶ to 10 ¹² pieces / g. The content of the chemical herbicide in the weed control composition according to the present invention varies depending on the formulation form, but is usually 0.05 to 15% by weight for granules, 1 to 50% by weight for flowable agents, and 1 to 1 for wettable powders. 90% by weight
And preferably 0.5 to 8% by weight for granules, 10 to 30% by weight for flowable agents, and 10 to 50 for wettable powders.
% By weight. On the other hand, the content of Drex Rera is
10 ² to 10 as spores per 1 g of the active ingredient of each composition
^The number is ¹⁵ , preferably 10 ⁶ to 10 ¹² .

As the auxiliary agents, surfactants, binders, stabilizers and the like which are usually used in agricultural and horticultural chemicals can be used alone or in combination as required. As a stabilizer,
For example, using an antioxidant or a pH adjusting agent,
A light stabilizer is also used. The content of the auxiliary agent is 0 to 80% by weight, and the content of the carrier is 100% by weight minus the active ingredient and the content.

When the weed-controlling agent and the weed-controlling composition of the present invention are used in the field, the amount of conidiospores of the bacterium of the genus Drexlera is 10 ² to 10 ¹⁵ spore / 10a, preferably 10 ⁷ to 10 ¹² spore / 10a.

The weed control composition according to the present invention has a temperature of 15 ° C.
One or more of the following chemical herbicides with one or two species of Dolex Rera monoceras species that are also infectious against Novier or less, or a fungicide that does not have antibacterial properties against the bacteria of the genus Dreyxella, insecticides, Not only can it be used in combination with agricultural chemicals such as plant growth regulators, fertilizers, soil conditioners, etc., but it is also possible to mix them.

The herbicides which can be mixed include the following herbicides. 2,4-dichlorophenoxyacetic acid (2,4-D), 2-methyl-4-chlorophenoxyacetic acid (MCPA), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 2- ( 2,4-dichlorophenoxy) propionic acid (dichlorprop),
2- (2-methyl-4-chlorophenoxy) propionic acid (mecoprop), 2- (2,4,5-trichlorophenoxy) propionic acid (fenopro)
p), 4- (2,4-dichlorophenoxy) butyric acid (2,4-DB), 4- (2-methyl-4-chlorophenoxy) butyric acid (MCPB), 2- (2-naphthoxy) propionanilide (naproanilide)
e), 2- (1-naphthoxy) N, N-diethylpropionamide (napropamid), (+)-2
-[4- (2,4-dichlorophenoxy) phenoxy] propionic acid methyl ester (diclofp-
methyl), 2- [4- (5-trifluoromethyl-2-pyridyloxy) phenoxy] propionic acid butyl ester (fluazifop), 2- [4-
(3-Chloro-5-trifluoromethyl-2-pyridyloxy) phenoxy] propionic acid methyl ester (h
alloxyfop), 2- [4- (3,5-dichloro-2-pyridyloxy) phenoxy] propionic acid-2
-Propynyl ester (chlorozifop-pr)
opynyl), 2- [4- (6-chloro-2-quinoxanyloxy) phenoxy] propionic acid ethyl ester (quizalofop-ethyl), 2-
[-4- (6-Chloro-2-benzoxazolyl) phenoxy] propionic acid ethyl ester (fenoxap
rop-ethyl), 2- [4- (6-chloro-2)
-Benzothiazolyloxy) phenoxy] propionic acid ester (fenthiaprop-ethyl),
2,3,6-trichlorobenzoic acid (2,3
3,6-TBA), 3,6-dichloro-2-methoxybenzoic acid (dicamba), 2,5-
Dichloro-3-aminobenzoic acid (amib
en), 3,5,6-trichloro-2-methoxybenzoic acid (tricamba), 4-chloro-2,2-dimethyl barrelanilide (monalid)
e), 3,4-dichloropropionanilide (pro
panil), 3,4-dichloro-2-methylacrylanilide (dicryl), 3,4-dichlorocyclopropanecarboxyanilide (cypromid),
3,4-dichloro-2-methyl-pentaneanilide (k
arsil), 3-chloro-2,4-dimethylpentaneanilide (solan), N- (1,1-dimethylpropynyl) -3,5-dichlorobenzamide (prop)
yzamide), N, N-dimethyl-2,2-diphenylacetamide (diphenamide),
N-naphthylphthalamic acid (Naptalam), N
-(1,1-Dimethylbenzyl (-2-bromo-tashari-butylacetamide (buromobutid
e), 2-benzothiazol-2-yloxy-N-
Methyl acetanilide, N
-[3- (1-Ethyl-1-methylpropyl) 5-isoxazolyl] -2,6-dimethoxybenzenamide (b
enzamizole), 1,1-dimethyl-3-phenylurea (fenuron), 3- (4-chlorophenyl) -1,1-dimethylurea (monuro)
n), 3- (4-chlorophenyl) -2,1,1-trimethylisourea (trimeturon), 3-
(4-Chlorophenyl) -1-methoxy-1-methylurea, 3- (4-chlorophenyl) -1-methyl-1- (1-methylpropyne-
2-yl) urea, 3- (4-bromophenyl) -1-methoxy-1-methylurea (m
etobromuron), 1- (2-methylcyclohexyl) -3-phenylurea (siduron),
1,1-Dimethyl-3- (3-trifluoromethylphenyl) urea, 3-
(3,4-Dichlorophenyl) -1,1-dimethylurea (diuron), 3- (3,4-dichlorophenyl) -1-methoxy-1-methylurea (linuro)
n), 3- (3,4-dichlorophenyl) -1-n-
Butyl-1-methylurea (neburon), 3-
(3-Chloro-4-methoxyphenyl) -1,1-dimethylurea (methoxuron), 3- (4-bromo-3-chlorophenyl) -1-methoxy-1-methylurea (chlorbromuron), 1- (4-
Difluorochloromethylmercapto-3-chlorophenyl) -3,3-dimethylurea (fluothiuro)
n), 3- (3-chloro-4-methylphenyl)-
1,1-dimethylurea
n), 3- [4- (4-chlorophenoxy) phenyl]
-1,1-Dimethylurea (chloroxuro)
n), 3- [4- (4-methoxyphenoxy) phenyl] -1,1-dimethylurea (difenoxuro)
n), 3- [3- (N-Taashary-butylcarbamoyloxy) phenyl] -1,1-dimethylurea (k
arbutylate), 3-benzoyl-3-
(3,4-dichlorophenyl) -1,1-dimethylurea, 1- (α, α
-Dimethylbenzyl) -3- (4-methylphenyl) urea (dymron), 3- (4-isopropylphenyl) -1,1-dimethylurea (isoprotur)
on), 3- (2-benzothiazolyl) -1,3-dimethylurea (methabenz thiazuro)
n), 3- (2-benzothiazolyl) 1-methylurea, 3- (hexahydro-4,7-methanoindan-5-yl) -1,1
-Dimethylurea (noruron), 3-cyclooctyl-1,1-dimethylurea (cycluro)
n), 1,3-dimethyl-3- (5-trifluoromethyl-1,3,4-thiadiazol-2-yl) urea (thiazfluron), 1- (5-ethylsulfonyl-1,3,4) -Thiadiazol-2-yl)-
1,3-dimethylurea (sulfodiazol),
3- [5- (1,1-dimethylethyl) -1,3,4
-Thiadiazol-2-yl] -1,3-dimethylurea, tebuthiuron, 3- (5-taashary-butylisoxazol-3-yl) -1,1-dimethylurea, isouron, 4 -[2-chloro-4- (3,3-dimethylureido) phenyl] -2-
Taashary-butyl-1,3,4-oxadiazoline-
5-one, 3- (5-tashari-butyl-1,3,4-thiadiazol-2-yl) -4-hydroxy-1-methyl-2-imidazolidone, 2-chloro- 4,6
-Bis (ethylamino) -1,3,5-triazine (s
imidazole), 2-chloro-4-ethylamino-6
-Isopropylamino-1,3,5-triazine (at
razine), 2-chloro-4,6-bis (isopropylamino) -1,3,5-triazine (propa
zine), 2-chloro-4-diethylamino-6-
Ethylamino-1,3,5-triazine (trieta)
zine), 2-chloro-4-ethylamino-6-ta-ash-butylamino-1,3,5-triazine (te
rbutylazine), 2- (2-chloro-4)
-Ethylamino-1,3,5-tyrazin-6-ylamino) -2-methylpropionitrile (cyanazin)
e), 2-chloro-4-cyclopropylamino-6-
Isopropylamino-1,3,5-triazine (pre
fox), 2- [2-chloro-4- (cyclopropylamino) -1,3,5-triazin-6-ylamino]
-2-Methylpropionitrile (procyazi)
n), 2-methoxy-4-secondary-butylamino-6-ethylamino-1,3,5-triazine (sec
bumeton), 2-methoxy-4,6-bis (isopropylamino) -1,3,5-triazine (pro
(methon), 2-methylthio-4,6-bis (ethylamino) -1,3,5-triazine (simry)
ne), 2-methylthio-4,6-bis (isopropylamino) -1,3,5-triazine (prometr
yne), 2-methylthio-4-methylamino-6-isopropylamino-1,3,5-triazine (amet)
ryne), 2-methylthio-4-ethylamino-6
-Taashary-butylamino-1,3,5-triazine, 2-methylthio-4-isopropylamino-6- (3-methoxypropylamino) -1,3,5-triazine (methoprotr)
yne), 2-methylthio-4- (1,2-dimethylpropyl) -6-ethylamino-1,3,5-triazine, 2-methylthio-
4-isopropylamino-6-methylamino-1,3,
5-triazine, 4-amino-6-taashary-butyl-3-methylthio-1,
2,4-triazin-5 (4H) -one (metrib
uzin), 2-methylthio-4,6-bis (isopropylamino) -1,3,5-triazine (dipro)
petryn), 2-taashary-butylamino-4
-Ethylamino-6-methoxyamino-1,3,5-triazine, 2-azido-4
-Isopropylamino-6-methylthio-1,3,5-
Triazine, 4-amino-3-methyl-6-phenyl-1,2,4-triazin-5 (4H) -one (metatron), 6-
Taashary-butyl-4-isobutylideneamino-1,
2,4-triazin-5 (4H) -one (isomet
hiozin), 3-cyclohexyl-6-dimethylamino-1-methyl-1,3,5-triazine-2,4
-(1H, 3H) -dione (hexazinone),
Ethyl-N- (4-chloro-6-ethylamino-1,
3,5-triazin-2-yl) -aminoacetate (eglinazine), ethyl-N- (4-chloro-6-isopropylamino-1,3,5-triazin-2-yl) -aminoacetate (proglinaz)
ine), 2-chloro-N-isopropylacetanilide (propachor), N-methoxymethyl-2 ', 6'-diethyl-2-chloroacetanilide (al)
achlor), 2-chloro-2 ', 6'-diethyl-N
-(Butoxymethyl) acetanilide (butachl)
or), 2-chloro-2'-ethyl-6'-methyl-N-
(2-Methoxy-1-methylethyl) acetanilide, N, N-diallyl-2
-Chloroacetamide,
2-chloro-2 ', 6'-dimethyl-N- (2-methoxyethyl) acetanilide (dimethyl),
2,6-dinitro-N, N-dipropyl-4-trifluoromethylaniline (trifluralin),
N-butyl-N-ethyl-2,6-dinitro-4-trifluoromethylaniline (benfluralin),
2,6-dinitro-N-propyl-N-cyclopropyl-4-trifluoromethylaniline (proflur
alin), N, N-diethyl-2,4-dinitro-
6-trifluoromethyl-m-phenylenediamine (d
initramin), 4-isopropyl-2,6-
Dinitro-N, N-dipropylaniline (isopro
palline), 2,6-dinitro-N-secondary-butyl-4-taashary-butylaniline (butr)
alin), 4- (methylsulfonyl) -2,6-
Dinitro-N, N-dipropylaniline (nitral
in), 3,4-dimethyl-2,6-dinitro-N-
1-Ethylpropylaniline (pendimethal
in), 3,5-dinitro-N, N-dipropylsulfanilamide (oryzalin), N-ethyl-
N- (2-methylallyl) -2,6-dinitro-4-
(Trifluoromethyl) aniline (ethalflur
alin), N, N-diethyl-2,4-dinitro-
6-trifluoromethyl-m-phenylenediamine (d
iethamine), 2-chloro-N- (4-methoxy-6-methyl-1,3,5-triazin-2-yl-aminocarbonyl) benzenesulfonamide (ch
lorosulfuron), methyl-2- [3-
(4-Methoxy-6-methyl-1,3,5-triazin-2-yl) ureidosulfonyl] benzoate (m
etsulfurone-methyl), methyl-
2- [3- (4,6-dimethylpyrimidin-2-yl)
Ureidosulfonyl] benzoate (sulfome)
Turon-methyl), methyl-2- [3-
(4-, 6-Dimethoxypyrimidin-2-yl) ureidosulfonyl] benzoate (bensulfuro)
n), ethyl-2- [3- (4-chloro-6-methoxypyrimidin-2-yl) ureidosulfonyl] benzoate (chlorinuron), 3-[(4-
Methoxy-6-methyl-1,3,5-triazine-2-
Ile) ureidosulfonyl] -2-thiophenecarboxylic acid (thiameturon), 3,7-dichloro-8-quinolinecarboxylic acid (quinchlora)
c), 3,6-dichloro-2-pyridinecarboxylic acid (clopyralid), α- (2-chlorophenyl) -α- (4-chlorophenyl) -5-pyrimidinemethanol (fenarimol), S, S-dimethyl- 2- (difluoromethyl) -4- (2-methylpropyl) -6-trifluoromethyl) -3,5-pyridinedicarbothioate (MON-15100, or MO)
N-15126), 4-chloro-s- (methylamino) -2- (3- (trifluoromethyl) phenyl) 3
(2H) -pyridazinone,
O, O-bis (1-methylethyl) -S- [2-
[(Phenylsulfonyl) amino] ethyl] sulfodithioate, (+)-2
-[4,5-Dihydro-4-methyl-4- (1-methylethyl) -5-oxo-1H-imidazol-2-yl] -5-methyl-3-pyridinecarboxylic acid (imaz
amethapyr), 3- [5- (1,1-dimethylethyl) -3-isoxazolyl) -4-hydroxy-1.
-Methyl-2-imidazolidione (busoxinon
e), 2- [1- (ethoxyimino) -butyl] -3
-Hydroxy-5- (2H-tetrahydrothiopyran-
3-yl) -2-cyclohexen-1-one (cycl
oxydim)

In particular, the weed control composition according to the present invention comprises
When used in combination with chemical herbicides for Nobie, such as diphenyl ether-based, anilide-based, and thiol carbamate-based chemical herbicides, the herbicidal effect is not conceivable when applied individually even at low temperatures of 15 ° C or lower. A moderate synergistic effect is obtained, and it is possible to control Nobie with a lower dose than expected.

The weed control composition according to the present invention comprises
Herbicides for broad-leaved weeds, for example, when used in combination with chemical herbicides such as sulfonylurea and triazine,
When the herbicidal effect is applied individually, a synergistic effect that is unthinkable can be obtained, the dose of the pathogenic microorganism that is one component of the present invention can be reduced, and the dose of the chemical herbicide can also be reduced. Moreover, it is possible to control all paddy field weeds due to the wide spectrum of weeding effects.

[0015]

EXAMPLES The following is a specific description of a mixed composition of a bacterium of the genus Dolexlera and a chemical herbicide according to the present invention, but the present invention is not limited thereto.

Test Example 1 Separation / Selection Method and Identification of Microorganism 1) Separation Method of Pathogenic Microbe
Surface sterilization was performed by preparing a tissue section of 0 to 20 mm, immersing it in a 70% ethyl alcohol aqueous solution for 1 to 2 seconds, and then immersing it in an aqueous sodium hypochlorite solution having an effective chlorine concentration of 2% for 10 minutes. The surface-sterilized lesion tissue was washed three times with sterile distilled water, placed on an agar medium, and then statically cultured for 72 hours in a thermostat at 10 ° C. After culturing, the hyphae tips of the grown filamentous fungi were subjected to single hyphae separation under a stereoscopic microscope and purely separated on a nutrient medium to obtain a surplus of 7,000 strains. Among them, promising strains, MH-111012, MH-
111014, MH-111027, MH-11103
2. The MH-111034 strain and the like have been deposited with the Institute of Microtechnology. The number of the Institute for Microcomputer Research is shown in the attached material. The isolated filamentous fungi were tested for pathogenicity to Nobie and safety to rice.

2) Assay of Pathogenicity of Isolated Microorganisms for Novier and Safety for Rice Nobie and rice (variety: Nipponbare) were aseptically grown in test tubes to obtain test materials. That is, surface sterilization was performed by soaking seeds of Nobie and rice in a 70% ethyl alcohol aqueous solution for 1 to 2 seconds and then in a sodium hypochlorite aqueous solution having an effective chlorine concentration of 2% for 10 minutes. The surface-sterilized seeds were washed three times with sterile distilled water, seeded in a previously sterilized medium in a test tube, and then raised in a chamber for growing plants up to the 1.5 leaf stage. On the other hand, each of the separated microorganisms was subjected to plate culture on a potato-dextrose agar medium, and a flora disk prepared by punching the outer edge of the flora with a sterilized cork bolla was used as an inoculum source. The flora disc was inoculated into the medium in a test tube in which novier and rice were grown, and 2
After culturing at 5 ° C. for 10 days, the pathogenicity of the microorganism to Novier and rice was evaluated according to the following four grades from − to +++. The results are shown in Table 1. +++; Death ++; Remarkable growth inhibition +; Slightly growth inhibition −; No effect Note that the MH-0003 strain is a genus of the genus Drexrella (Drec).
hslera sp.) MH-0007 strain is a genus of Phoma (Phom
a sp.) MH-0011 strain is a Fusarium sp.
um sp.), the MH-0015 strain is of the genus Drexlera (Drechslera monoceras), and the MH-9011 strain is of the genus Drechslera (Drechslera monoceras), and both were tested for comparison.

[0018]

3) Identification of Microorganisms Strains showing remarkable pathogenicity to Nobie and no effects on rice were identified.
As a result, MH-111012, MH-11014,
MH-111027, MH-111032, MH-1
The 11034 strain was plated on a malt agar medium at 28 ° C. for 7 days to give colonies having a diameter of 65 to
It reached 75 mm and showed irregular growth. The color of the colony is grayish black, the conidia have traces, and the size is 15 ~
The shape is 17.5 μm, the length is 87.5 to 127.5 μm, and the shape is slightly curved. Most of the conidium septa are 5
With seven, the conidia peduncle is straight. From the above features, MH-111012, MH-11014, MH-
111027, MH-111032, MH-11103
All four strains are Drexrella monoceras (Drechs
Lera monoceras). On the other hand, no pathogenicity was shown against Nobie, MH-0003 was genus Drechslera sp., MH-0011 strain was Fusarium sp., And MH-0007 strain was Phoma sp. ) Was identified. The above identification is based on M.
B. Ellis (1971) Demariaceus Hyphomycetes p.608, C
ommonwealth Mycological Institute, Kew, England
MB Ellis (1976) More Demariaceus Hyphomycetes
p.507, Commonwealth Mycological Institute, Kew, Eng
I went to land. MH-111012, MH-
111014, MH-111027, MH-11103
2, 5 strains of MH-111034, etc. have been deposited with the Institute of Microtechnology. The number of the Institute for Microcomputer Research is shown in the attached material. Drechslera spp. According to the present invention,
The National Institute of Preventive Health does not have a description as a pathogen in the safety management rules for pathogens, etc., and is guaranteed to be safe for humans and animals.

Test Example 2 Controlling effect of Nobie by the genus Dolexrella The genus Dolexrella isolated from the natural world was inoculated on an oat mill agar medium and statically cultivated at 25 ° C. for 7 days.
Thereafter, the aerial hyphae were removed with distilled water to promote the formation of conidia. The conidia obtained were 10 ⁸ and 1
0 of ⁵ / ml and made so 0.02% Triton X-10
0 (trade name / manufactured by Rohm and Haas Co.) was suspended in an aqueous solution to prepare a weed-controlling agent containing a bacterium of the genus Dreyxella. On the other hand, seeds of Nobie and rice (cultivar; Nipponbare) were sown in a paddy soil filled in a pot of 1 / 10000a, and seedlings were raised to the 1.5 leaf stage. After the water depth is about 5 cm, 5 ml of the above weed control agent containing conidiospores of the genus Dreyxella is added dropwise and cultivated for 10 days in the artificial climate room at 15 ° C during the daytime, then the genus Dreyxella Test Example 1 for the effect of fungi on Nobie and rice
It evaluated by the same criteria as. The results are shown in Table 2. +++; Death ++; Remarkable growth inhibition +; Slight growth inhibition −; No effect

[0021] As a result of the test, MH- of the genus Dolex Rera according to the present invention
111012, MH-11014, MH-11102
7, MH-111032, MH-111034, 15
MH-0015, MH against novier even under conditions below ℃
The herbicidal activity was 100 to 1000 times better than that of -9011, and the safety against rice was confirmed.

Test Example 3 In the same manner as in Test Example 1-2), the pathogenicity of the microorganism of the present invention against various Echinochloa species and rice was evaluated. That is, Corona spp.
Orychocola spp. Of Echinochloa spp. (Japanese name: Tainubie), Variant formocensis spp. Of Cryptomeria spp. Of Echinocloa spp. (Himetanuibie spp. Of Japan), Varieties of Cruzgalli spp. Of Echinocloa spp. Name: Himeinubie), rice cultivated species Nipponbare (cultivar name), Sasanishiki (cultivar name), Koshihikari (cultivar name) were tested. Ten days after the inoculation, the pathogenicity of the microorganism of the present invention against Nobie and rice was evaluated, and the results are shown in Table 3.

[0023] +++; Death ++; Remarkable growth inhibition +; Slightly growth inhibition −; No effect As a result of the test, MH111012, MH111014, MH11 of the Dorexella monoceras species according to the present invention
The strains 1027, MH111032 and MH111034 exhibited strong herbicidal activity against all species and varieties of the genus Echinochloa even under low temperature conditions of 15 ° C. or lower, and showed excellent herbicidal activity, and further, safety against rice was confirmed.

Test Example 4 Effect of chemical herbicide on various biological properties of Dorexella spp. The flora disk of Dorexella spp. Produced by the method shown in Test Example 1-2) was used as a chemical herbicide CNP (Table). Medium A), mefenacet (E in the table), pretilachlor (I in the table), benchocarb (L in the table), bensulfuron (P in the table), and the like were inoculated on potato-dextrose agar medium containing 500 ppm each. After static culture was carried out at 25 ° C. for 5 days, the diameter of the formed flora was measured and used as mycelial extension. In addition, Triton X-100 of the spore suspension of the bacterium of the genus Dolex Rera prepared by the method shown in Test Example 2
The aqueous solution was replaced with a potato dextrose liquid medium containing 500 ppm of each chemical herbicide, and shake culture was carried out at 25 ° C. for 24 hours. After culturing, the presence or absence of spore germination was observed under a microscope, and the spore germination rate was calculated. The effects of each chemical herbicide on the hyphal elongation and spore germination rate of the bacterium of the genus Dolexlera were expressed as a percentage with respect to the control group containing no chemical herbicide, and the results are shown in Table 4. As shown in Tables 4 and 1 of the present invention, the strains of the genus Dreyxella showed almost no inhibition of hyphal elongation and spore germination by chemical herbicides.

[0025]

[0026]

Test Example 5 Controlling effect of Novier by a mixed composition of a bacterium of the genus Dolexrella and a chemical herbicide CNP. The genus Dolexrella isolated from the natural world was inoculated on an oat mill agar medium, and statically cultured at 25 ° C. for 7 days. went.
Thereafter, the aerial hyphae were removed with distilled water to promote the formation of conidia. The conidia thus obtained were suspended in a 0.02% Triton X-100 (trade name / produced by Rohm and Haas Co.) aqueous solution so as to have a predetermined concentration, and the bacterium of the genus Drexella sp. A weed control agent as a component was prepared. Regarding CNP, 30% of 9% granules is used.
1 mg was weighed and used as a chemical herbicide. On the other hand, seeds of Nobie and rice (cultivar; Nipponbare) were sown in a paddy soil filled in a pot of 1 / 10000a, and seedlings were raised to the 1.5 leaf stage. After putting the water in a flooded state with a water depth of about 5 cm,
The above weed-controlling agent 5 containing conidia of the genus Drexella
ml was added dropwise and the chemical herbicide CNP was treated at the same time. After culturing for 10 days in an artificial climate room at 15 ° C. in the daytime and at night, the effect of the Drexella spp. Of the present invention and the chemical herbicide on Nobie and rice was evaluated according to the same criteria as in Test Example 1. The results are shown in Table 5. +++; Withered ++; Remarkable growth inhibition +; Slightly growth inhibition −; No effect Table-5 shows only one case, but the synergistic effect of the combination of the bacterium of the genus Dolexlera and the chemical herbicide of the present invention is remarkable. Showed action.

[0028]

Formulation Example and Test Example Next, formulation examples and herbicidal activity test examples of the herbicide according to the present invention will be shown.

Formulation Example-1 (Granule) After thoroughly mixing 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite, Granules of 1 g of conidia of Drexrella spp. (MH-111034 strain)
A spore suspension containing 10 ⁹ per spore was added for wetting and then extruded and granulated on a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

Formulation Example-2 (granules) After thoroughly mixing 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite, Granules of conidia of Drexrella spp. (MH-111012 strain) 1g
A spore suspension containing 10 ⁸ per spore was added for wetting, and then extrusion granulated with a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

Formulation Example-3 (granules) After thoroughly mixing 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite, Granules of conidia of the genus Dolex Rera (MH-111027 strain) 1 g
A spore suspension containing 10 ⁹ per spore was added for wetting and then extruded and granulated on a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

Formulation Example-4 (Wettable powder) Neoperex (trade name / manufactured by Kao) 2% by weight, Triton (X-100) 2% by weight, white carbon 5% by weight
Was impregnated with a spore suspension containing 10 ¹⁰ conidiospores of the genus Dolex Rera (MH-11114 strain) per 1 g of the wettable powder, air-dried, and then 91% by weight of diatomaceous earth was well pulverized and mixed to obtain the wettable powder. Obtained.

Formulation Example-5 (Wettable powder) Neoperex (trade name, manufactured by Kao; sodium dodecylbenzenesulfonate): 2% by weight, Neugen EA80
(Trade name, manufactured by Sanyo Kasei; polyoxyethylene nonylphenyl ether): 1% by weight, white carbon: 5% by weight, and diatomaceous earth 92% by weight.
H-111032 strain) 1 conidiospore per 1 g of wettable powder
After impregnation with a spore suspension containing 0 ⁹ cells and air-drying, the mixture was thoroughly pulverized and mixed to obtain a wettable powder.

Formulation Example-6 (Flowable Agent) 10 wt% of sun extract (P252) dissolved in 80 wt% of water was wet-milled and mixed, and then Drexella spp.
H-111012 strain) conidiospore flowable agent 1m
0.4 wt% of Kelzan S (trade name, manufactured by Kelco) dissolved in 9.6 wt% of a spore suspension containing 10 ¹⁰ cells per liter was added and mixed to obtain a flowable agent.

Formulation Example-7 (Flowable agent) Sun extract P252 dissolved in 70% by weight of water (trade name, the same as above): 10% by weight, and further Drexella spp. (M)
H-111034 strain) conidiospore flowable agent 1m
10% by weight of a spore suspension containing 10 ¹⁰ per liter was mixed, wet-milled and mixed, and then 0.2% by weight of Kelzan S (trade name, manufactured by Kelco; xanthan gum) dissolved in 9.8% by weight of water was added. And mixed to obtain a flowable agent.

Formulation Example-8 (Dry flowable agent) Sodium alkylbenzene sulfonate: 15% by weight and polypropylene glycol polyethylene glycol ether: 85% by weight
111012 strain) conidiospores as a dry flowable agent 1
A dry flowable agent was obtained by mixing 10 ¹⁰ pieces per g.

Formulation Example-9 (powder) Emulgen 910 (trade name, manufactured by Kao; polyoxyethylene nonylphenyl ether): 0.5% by weight and kaolin clay: 99.5% by weight were well pulverized and mixed, and further Drexella spp. The conidial spores of the bacterium (MH-111012 strain) were mixed at 10 ⁸ per 1 g of the dust to obtain a dust.

Formulation Example-10 (Emulsion) Lecithin: 5% by weight, heavy white oil 94% by weight
Conidiospores of the genus Dolex Rera (MH-111012 strain) were suspended in 10 ¹⁰ per 1 g of the emulsion, and an equal amount of 1% by weight of Triton X-100 was added and mixed to emulsify to obtain an emulsion. .

Formulation Example-11 (Granule) 9% by weight of finely ground chemical herbicide, CNP (A in the table), 2% by weight of Neoperex (trade name / manufactured by Kao), Sun Extract P252 (trade name / 2% by weight of Sanyo Kokusaku Pulp) and 87% by weight of zeolite are mixed well, and then a spore suspension containing 10 ⁸ conidiospores of the genus Dreyxella (MH-11114 strain) per 1 g of granules is added to moisten the mixture. ,
Next, it was extruded and granulated with a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

Formulation Example-12 (Granule) 3.5% by weight of a chemical herbicide, butachlor (H in the table), Neoperex (trade name / manufactured by Kao) 2% by weight, Sun Extract P252 (trade name / sanyo) 2% by weight of zeolite and 92.5% by weight of zeolite are mixed well, and then a spore suspension containing 10 ⁸ conidiospores of the genus Dolex Rera (MH-111027 strain) per 1 g of granules is added and wet. Then, it was extruded and granulated by a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

Formulation Example-13 (Granule) 4% by weight of finely ground chemical herbicide mefenacet (E in the table), 2% by weight of Neoperex (trade name / manufactured by Kao), Sun Extract P252 (trade name / Sanyo Kokusaku Pulp)
After thoroughly mixing 2% by weight and 92% by weight of zeolite, a spore suspension containing 10 ⁸ conidiospores of the genus Dolex Rera (MH-111032 strain) per 1 g of granules was added to moisten it, and then small injection It was extruded and granulated by a molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

Formulation Example-14 (Granule) Finely ground chemical herbicide, benzosulfuron (in table; P)
0.2% by weight, NeoPerex (trade name / made by Kao) 2
Wt%, Sun extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp) 2% by weight, and 95.8% by weight of zeolite were thoroughly mixed, and then a bacterium of the genus Dolex Rera (MH-111034 strain)
The spore suspension containing 10 ⁸ per 1 g of granules of the conidia was added and moistened, and then extruded and granulated by a small injection molding machine. After air-drying and crushing this, 0.3 with a granulator
~ 2 mm granules were obtained.

Formulation Example-15 (Granule) 0.3% by weight of finely ground chemical herbicide, pyrazosulfuron-ethyl (Q in the table), Gosenol GL-0
5s (Nippon Gosei Kagaku Co., Ltd. PVA): 2% by weight, sun extract
P-252 (sodium lignin sulfonate manufactured by Sanyo Kokusaku Pulp Co., Ltd.): 2% by weight and clay: 95.7% by weight were mixed well, and conidiospores of the genus Dolex Rera (MH-111027 strain) were granulated. A spore suspension containing 10 ⁸ per gram was added and moistened, and then extrusion granulated by an injection molding machine. This was air-dried and disintegrated, and then granulated with a granulator to 0.3 to 1 mm to obtain granules.

Formulation Example-16 (Granule) 1.5% by weight of finely ground chemical herbicide, oxadiazone (D in the table), Gosenol GL-05s (PVA, Nippon Synthetic Chemical Industry Co., Ltd.): 2 % By weight, Sun Extract P-252 (Sanyo Kokusaku Pulp Co., Ltd. lignin sulfonate sodium): 2
Wt% and clay: 94.5 parts by weight, after mixing well,
A conidial spore of a bacterium of the genus Dolex Rera (MH-111032 strain) was added to a spore suspension containing 10 ⁸ per 1 g of granules to moisten it, and then extruded and granulated by an injection molding machine. This was air-dried and disintegrated, and then granulated with a granulator to 0.3 to 1 mm to obtain granules.

Formulation Example-17 (Granule) 1. The finely ground chemical herbicide, simethrin (S in the table) was added to 1.
5% by weight, Gosenol GL-05s (Nippon Gosei Kagaku Co., Ltd. PVA): 2% by weight, Sun Extract P-252 (Sanyo Kokusaku Pulp Co., Ltd. lignin sulfonate sodium): 2% by weight and clay: After thoroughly mixing 94.5% by weight, a spore suspension containing 10 ⁸ conidiospores of the genus Dolex Rera (MH-111012 strain) per 1 g of granules was added to moisten it, and then extruded by an injection molding machine. Granulated. This was air-dried and disintegrated, and then granulated with a granulator to 0.3 to 1 mm to obtain granules.

Formulation Example-18 (Granule) 6% by weight of chemical herbicide, molinate (N in the table), 2% by weight of Neoperex (trade name / manufactured by Kao), Sun Extract P
252 (trade name / manufactured by Sanyo Kokusaku Pulp) and 2% by weight of zeolite and 90% by weight of zeolite were well mixed, and then a spore suspension containing 10 ⁸ conidiospores of the genus Dolex Rera (MH-11114 strain) per 1 g of granules. Was added to wet and then extruded and granulated with a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

Formulation Example-19 (Wettable powder) Chemical herbicide, 30% by weight of chloromethoxynil (B in the table), 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Triton (X-100) %, 5% by weight of white carbon, Drexlera spp. (MH-111034 strain)
The spore suspension containing 10 ⁹ per 1 g of wettable powder was impregnated with the conidia of Example 1 and then 61% by weight of air-dried diatomaceous earth was well pulverized and mixed to obtain a wettable powder.

Formulation Example-20 (Wettable Powder) Chemical herbicide, 40% by weight of propanil (J in the table), 2% by weight of Neoperex (trade name / manufactured by Kao), Triton (X)
-100) 2% by weight and white carbon 5% by weight were impregnated with a spore suspension containing conidiospores of the genus Dreyxella (MH-111012 strain) at 10 ⁹ per gram of wettable powder, and then air-dried. 51 wt% of diatomaceous earth was well pulverized and mixed to obtain a wettable powder.

Formulation Example-21 (wettable powder) Chemical herbicide, Benchocarb (in table; L) 20% by weight,
Neoperex (product name / Kao) 2% by weight, Triton (X-100) 2% by weight, white carbon 5% by weight
Was impregnated with a spore suspension containing 10 ⁹ conidiospores of the genus Dolex Rera (MH-11114 strain) per 1 g of the wettable powder, and 71% by weight of air-dried diatomaceous earth was well pulverized and mixed to obtain the wettable powder. Obtained.

Formulation Example-22 (Wettable powder) Chemical herbicide, naproanilide (in table; F) 40% by weight,
Neoperex (trade name, manufactured by Kao; sodium dodecylbenzenesulfonate): 2% by weight, Neugen EA80
(Commercial name, Sanyo Kasei; polyoxyethylene nonylphenyl ether): 1% by weight, white carbon: 5% by weight and diatomaceous earth 52% by weight, a bacterium of the genus Dolex Rera (M
H-111027) conidiospores per 1 g of wettable powder
After impregnation with a spore suspension containing 0 ^{9 of} them, the mixture was thoroughly pulverized and mixed to obtain a wettable powder.

Formulation Example-23 (Wettable powder) Chemical herbicide, dimepiperate (M in the table) 20% by weight,
Sodium alkylbenzene sulfonate: 2% by weight, polyoxyethylene alkylphenyl ether: 1% by weight
And Sikrite: 77% by weight was impregnated with a spore suspension containing 10 ⁹ conidiospores of the genus Dreyxella (MH-111012 strain) per 1 g of wettable powder, and then pulverized and mixed well to give a wettable powder. Obtained.

Formulation Example-24 (Wettable powder) Chemical herbicide, clomeprop (K in table): 40 parts by weight, white carbon: 5% by weight, polyoxyethylene alkylphenyl ether sulfate ammonium salt: 6% by weight, lignin sulfone Sodium acidate: 2% by weight and diatomaceous earth: 47% by weight were well pulverized and mixed using a Jet-0-mizer, and contained 10 ⁹ conidia of Drexella spp. (MH-111032 strain) per 1 g of wettable powder. After impregnation with the spore suspension, it was thoroughly pulverized and mixed to obtain a wettable powder.

Formulation Example-25 (Flowable Agent) 45% by weight of finely ground chemical herbicide, CNP (in table; A)
And sun extract dissolved in 35% by weight of water (P252)
10 wt% was wet pulverized and mixed, and then Kerzan S was dissolved in 9.6 wt% of a spore suspension containing 10 ⁹ conidiospores of the genus Dolex Rera (MH-111027 strain) per 1 ml of the flowable agent (trade name, Kelco) 0.4% by weight
Was added and mixed to obtain a flowable agent.

Formulation Example 26 (Flowable Agent) Chemical herbicide, pretilachlor (in the table; I) 10% by weight
And sun extract dissolved in 70% by weight of water (P252)
Kelzan S (trade name, 10% by weight of which was wet pulverized and mixed, and then dissolved in 9.6% by weight of a spore suspension containing 10 ⁹ conidiospores of the genus Dolex Rera (MH-111012 strain) per 1 ml of a flowable agent. Kelco) 0.4% by weight
Was added and mixed to obtain a flowable agent.

Formulation Example-27 (Flowable agent) Chemical herbicide, Benchocarb (in table; L) 30% by weight,
Sodium lignin sulfonate: 2% by weight, xanthan gum: 0.3% by weight, and polyoxyethylene alkylaryl ether: 1% by weight, to which 56.7% by weight of water was added to further add a bacterium of the genus Drexrella (MH-111027 strain). 10% by weight of a spore suspension containing 10 ⁹ conidia per ml of the flowable agent was mixed, and then finely ground using a sand grinder to obtain a flowable agent.

Formulation Example-28 (Flowable Agent) Chemical herbicide, sun extract P252 (trade name, same as above) dissolved in 30% by weight of Esprocurve (O in the table; 40% by weight) and 10% by weight, In addition, the genus Drexella
H-111032 strain) conidiospore flowable agent 1m
10% by weight of a spore suspension containing 10 ⁹ per liter was mixed, wet-pulverized and mixed, and then dissolved in 9.6% by weight of water, Kelzan S (trade name, manufactured by Kelco; xanthan gum): 0.4% by weight. In addition, they were mixed to obtain a flowable agent.

Formulation Example-29 (Dry flowable agent) Finely ground chemical herbicide, pyrazolate (R in the table) 60
% By weight, sodium alkylbenzene sulfonate: 5% by weight and polypropylene glycol polyethylene glycol ether: 35% by weight, and conidiospores of the genus Dolex Rera (MH-11114 strain) as a chemical herbicide 1
A dry flowable agent was obtained by mixing 10 ⁹ per g.

Formulation Example-30 (powder) Finely ground chemical herbicide, bensulfuron (in table; P)
0.2% by weight, Emulgen 910 (trade name, manufactured by Kao; polyoxyethylene nonylphenyl ether): 0.5% by weight and kaolin clay: 99.3% by weight were well pulverized and mixed, and further, a bacterium of the genus Dolex Rera (MH- 111034
Conidiospores of (strain) were mixed at a rate of 10 ⁸ per 1 g of powder to obtain a powder.

Formulation Example-31 (powder) Finely ground chemical herbicide, mefenacet (in the table; E) 4
% By weight, Emulgen 910 (trade name, manufactured by Kao; polyoxyethylene nonylphenyl ether): 1% by weight, sodium ligninsulfonate: 3% by weight, polyoxyethylene alkylaryl ether: 2% by weight and clay:
90% by weight is mixed and pulverized, and the bacterium of the genus Dolex Rera (M
H-111012) conidia 10 g / g powder
⁹ pieces were mixed to obtain a powder.

Formulation Example-32 (Emulsion) Chemical herbicide, 1% by weight of bensulfuron (in the table; P), lecithin: 5% by weight, 94% by weight of heavy white oil, and a portion of the genus Dolex Rera (MH-111012 strain). 10 ⁹ live spores were suspended per 1 ml of an emulsion, and an equal amount of 1% by weight of Triton X-100 was added and mixed to emulsify to obtain an emulsion.

Formulation Example-33 (Emulsion) Chemical herbicide, 2% by weight of bifenox (C in the table), lecithin: 5% by weight, 92% by weight of heavy white oil, and congenitality of the genus Dolex Rera (MH-111027 strain). 10 ¹⁰ spores were suspended per 1 ml of an emulsion, an equal amount of 1% by weight of Triton X-100 was added, mixed and emulsified to obtain an emulsion. In addition to the above formulation examples, formulations were similarly performed for combinations of each conidia and each chemical herbicide. The formulation is not limited to the above examples.

Example 1 Herbicidal action against novier of a weed control composition in which the novel microbial strain of the present invention and a chemical herbicide are combined-granules-Novier seeds are sown in paddy soil packed in a pot of 1 / 1000a, Raised seedlings up to the 1.5 leaf stage. Water depth is about 5
30 mg of each granule prepared in the above formulation examples 11 to 18 (corresponding to 1/10 of standard application amount)
Was treated and cultivated in a constant temperature room at 15 ° C. both during the day and at night.
20 days after application, the number of remaining individuals was counted, and the control rate against Nobie was shown in Tables 1 to 6 in accordance with the following formula. In the table, A to O represent the following herbicides. A: CNP, B: chloromethoxynil, C: biphenox, D: oxadiazine, E: mefenacet, F: naproanid, G; NSK-850, H; butachlor, I; protilachlor, J; propanil, K; clomeprop, L: Bench-o-curve, M: Dimepiperate, N: Molinate, O: Esplocurve,

[0064]

[0065]

[0066]

As shown in Table 6, a mixture composition of the novel microbial strain according to the present invention and a diphenyl ether chemical herbicide, CNP, clomethoxynil, bifenox, etc., or a diazine herbicide, oxadiazone, etc. was applied individually. The herbicidal activity was synergistically increased as compared with the case of. From Table 6-2, the novel microbial strain of the present invention, anilide chemical herbicide, mefenacet naproanilide, NS
The mixed composition of K-850, butachlor, protilachlor, propanil, clomeprop and the like synergistically increased herbicidal activity as compared with the case where each composition was applied alone. From Table 6 shown in Table 6, the composition of the novel microbial strain according to the present invention and the thiol carbamate chemical herbicide, benchocarb, dimepiperate, molinate, esprocarb, etc., have synergistic herbicidal activity as compared with the case where they are applied individually. Increased significantly. From the above results, a novel microbial fungus having pathogenicity to Novie and conventionally used as a herbicide for Novie, diphenyl ether chemical herbicides, anilide chemical herbicides,
A mixed composition with a thiol carbamate-based chemical herbicide, a diazine-based chemical herbicide, or the like shows a remarkable synergistic effect. As a result, it has become possible to sufficiently control Nobies even at low temperature conditions of 15 ° C. or lower, and even when the conventionally applied chemical herbicides are used at 1/10 to 1/10.

Example 2 Herbicidal action of a novel weed control composition of the present invention and a chemical herbicide against noviae against weeds-flowable agent-nobue seeds are sown in paddy soil in 1 / 1000a pots, Raised seedlings up to the 1.5 leaf stage. Water depth is about 5
1 μl of the flowable agent (corresponding to 1/10 of the standard application amount) prepared in Formulation Examples 25 to 28 described above was treated in a flooded pot of cm, and cultivated in a constant temperature room at 15 ° C. for both daytime and nighttime. 20 days after application, the number of remaining individuals was counted, and the control rate against Nobie was shown in Tables 1 to 7 according to the following formula. From Table 7 below, the mixed composition of the novel microbial strain according to the present invention, the diphenyl ether chemical herbicide, and CNP is
The herbicidal activity was synergistically increased compared with the case of applying each alone. From Table 7-2, the composition of the novel microbial strain of the present invention and the chemical chemical herbicide of anilide, mefenacet, and protilachlor had synergistically enhanced herbicidal activity as compared with the case where each composition was applied alone. . From Table 7-3, a novel microbial strain according to the present invention and a thiol carbamate herbicide,
The mixed composition of bench ocarb and molinate synergistically increased the herbicidal activity as compared with the case where each composition was applied alone even at a low temperature condition of 15 ° C or lower.

[0069]

[0070]

[0071]

Example 3 Herbicidal action of Noboe by the composition for controlling weeds in which the novel microbial strain of the present invention and a chemical herbicide are combined-emulsion-Sowing of seeds of Novier in paddy soils packed in 1 / 1000a pots was performed and 1 The seedlings were raised to the 5th leaf stage. Water depth is about 5
cm in a submerged pot, treated with 1.5 ml of the emulsion prepared as in Formulation Examples 32 to 33 (corresponding to 1/10 of the standard application amount), and cultivated in a constant temperature room at 15 ° C. for both daytime and nighttime. did. 20 days after application, the number of remaining individuals was counted, and the control rate against Nobie was shown in Table 8 by the following formula. In the table, A to O represent the following herbicides. A: CNP, I: Protilachlor, E: Mefenacet, L: Benchocarb, Table 8 shows that the mixed composition of the novel microbial strain according to the present invention, the diphenyl ether chemical herbicide, and CNP is the same as when it is applied individually. In comparison, herbicidal activity increased synergistically.
The mixed composition of the novel microbial strain of the present invention and the anilide chemical herbicide, mefenacet, and protilachlor synergistically increased herbicidal activity as compared with the case where each composition was applied alone. The mixed composition of the novel microbial strain according to the present invention and the thiol carbamate herbicide, Benchocarb and Mori 1-nate synergistically increased herbicidal activity as compared with the case where each composition was applied alone.

[0073]

Example 4 Herbicidal action of a novel weed control composition of the present invention and a chemical herbicide against weeds and broad-leaved weeds Weed control composition Nobye, Japanese eel, and Hera-modaka seeds 1 / 1000a
The seeds were sown in the paddy soil filled in the pot, and seedlings were raised until the 1.5 leaf stage. 1.5 mg of each mixed agent prepared in the above formulation examples 11 to 33 in a submerged pot with a water depth of about 3 cm
(Corresponding to 1/10 of the standard application rate) was treated and cultivated in a constant temperature room at 15 ° C. both during the day and at night. 20 days after application, the number of remaining individuals was counted, and the control effect on Nobie, Konagi and Japanese mosquito was shown in Table 9-1 to Table 9-3 as the control rate by the following formula. In the table, P to S represent the following herbicides. P: Bensulfuron-methyl, Q; Pyrazosulfuron-ethyl, R; Pyrazolate, S; Cimethrin,

[0075]

[0076]

[0077]

From Table 9-1, the novel microbial strain according to the present invention and a sulfonylurea chemical herbicide, bensulfuron-methyl, pyrazosulfuron-ethyl, etc., or a diazine herbicide, pyrazolate, etc., or a triazine herbicide The composition mixed with the agent, cimetrin, etc. synergistically increased herbicidal activity against Nobies even at a low temperature of 15 ° C or lower, as compared with the case where each composition was applied alone. Table 9-2 and 9
From Table-3, a novel microbial strain according to the present invention and a sulfonylurea-based chemical herbicide, bensulfuron-methyl, pyrazosulfuron-ethyl, etc., or a diazine-based herbicide, pyrazolate, etc., or a triazine-based herbicide, cimetrin, etc. The effect of the mixed composition of (1) is slightly enhanced even on broad-leaved weeds such as eels and Japanese style mosquitoes, as compared with the case where each composition is applied alone. From Table 9-2 and Table 9-3,
A novel microbial strain according to the present invention and a sulfonylurea-based chemical herbicide, bensulfuron-methyl, pyrazosulfuron-ethyl, etc., or a diazine-based herbicide, pyrazolate, etc., or a triazine-based herbicide, a mixed composition with cimetrin, etc. As compared with the case of applying each alone, it showed a sufficient weed control effect against broad-leaved weeds such as Japanese eels and Japanese mosquitoes. From the above results, the novel microorganism strain of the present invention having pathogenicity to Nobie was used as a herbicide for conventional broadleaf weeds, sulfonylurea-based chemical herbicides, bensulfuron-methyl, pyrazosulfuron-ethyl, etc., or diazine-based. Herbicides, pyrazolates, or the like, or a triazine-based herbicide, a mixed composition with simethrin, etc., enhances the weed-controlling effect on Nobye, and also for broad-leaved weeds, the spectrum spreads, and although slightly With the enhancement of the effect, it became practical. Further, by using the herbicide for broad-leaved weeds in combination, it is possible to reduce the amount of conidia of the novel microorganism of the present invention, as shown in Table 10, and it is possible to reduce the production cost.

Example 5 Reduction of Conidia Content Applied by Synergistic Action of the Novel Microbial Strain of the Present Invention and Chemical Herbicides Novie seeds were sown in paddy soil packed in 1 / 1000a pots up to 1.5 leaf stage. Raised seedlings. Water depth is about 5
A predetermined amount of conidia and a broad-leaf herbicide bensulfuron (corresponding to 1/10 of the standard application amount) were treated in a flooded pot of cm, and cultivated in a constant temperature room at 15 ° C. during the day and night. 20 days after application, the number of remaining individuals was counted, and the control effect against Nobie was shown in Table 10 as the control rate according to the following formula.

[0080]

[0081]

INDUSTRIAL APPLICABILITY The novel strain of the genus Dolerxella monoceras according to the present invention is found to be pathogenic to all species of the genus Echinochloa, which is a nodule of weeds, even at 15 ° C. or lower,
Practical herbicidal activity against Nobie was shown including the rice planting time. Furthermore, it was confirmed to be extremely safe for cultivated plants such as rice. The microorganism according to the present invention is selected from natural microorganisms, and is safe without concern about environmental pollution, which is a concern with organic synthetic pesticides. Further, a mixed composition of a new strain of the genus Dolexrella and a chemical herbicide according to the present invention, a herbicide application rate which cannot be controlled by each alone, shows a sufficient control effect,
It became possible to reduce the herbicide application rate. Furthermore, due to the synergistic effect of the mixture of Drexrella spp. And a chemical herbicide,
It was also possible to reduce the amount of conidiospores of the genus Dolex Rera applied, and to reduce the production cost. As described above, the weed-controlling agent and the weed-controlling composition according to the present invention not only contribute to crop production, but also contribute to avoiding environmental pollution and the emergence of drug-resistant weeds, which have recently become a problem.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification code Internal reference number FI technical display location (C12P 1/02 C12R 1: 645) (72) Inventor Emi Niimi 1144 Togo, Mobara-shi, Chiba Mitsui Toatsu Kagaku Co., Ltd. (72) Inventor Hitoshi Takanaka 1144 Togo, Mobara-shi, Chiba Mitsui Toatsu Kagaku Co., Ltd. (72) Inventor Tomoko Hikita 1144 Togo, Mobara-shi, Chiba Mitsui Toatsu Kagaku Co., Ltd.

Claims (26)

[Claims] 1. A genus Echinocloa s at a temperature of 15 ° C. or lower.
pp.) and a pathogenic microorganism Drechslera monoceras (Drechslera monoceras). 2. The novel strain according to claim 1, which is one of the pathogenic microorganisms of the genus Drechslera monoceras according to claim 1, which does not show pathogenicity to cultivated plants. 3. A new strain of the pathogenic microorganism Drechslera monoceras is MH11101.
The new strain according to claim 1, which is 2 (FERM BP-3865). 4. A new strain of the pathogenic microorganism Drechslera monoceras is MH11101.
4 (FERM BP-3866), The new strain according to claim 1. 5. A new strain of the pathogenic microorganism Drechslera monoceras is MH11102.
The new strain according to claim 1, which is 7 (FERM BP-3867). 6. A new strain of the pathogenic microorganism Drechslera monoceras is MH11103.
2 (FERM BP-3868), The new strain according to claim 1. 7. A new strain of the pathogenic microorganism Drechslera monoceras is MH11103.
4 (FERM BP-3869), The new strain according to claim 1. 8. The plant does not show pathogenicity and has a temperature of 15 ° C.
In the following, the pathogenic microorganism Drech-slera moth (Drech-slera mop) which is pathogenic to Echinocloa spp.
noceras) and a new weed control agent. 9. A new strain of the pathogenic microorganism Drechslera monoceras is MH11101.
2 (FERM BP-3865), which contains the new strain of claim 8 as a weed control agent. 10. A new strain of the pathogenic microorganism Drechslera monoceras is MH1110.
No. 14 (FERM BP-3866), which contains the novel strain of claim 8 as a weed control agent. 11. A new strain of the pathogenic microorganism Drechslera monoceras is MH1110.
27 (FERM BP-3867), which contains the new strain of claim 8, which is a weed control agent. 12. A new strain of the pathogenic microorganism Drechslera monoceras is MH1110.
32 (FERM BP-3868) containing the new strain of claim 8 as a weed control agent. 13. A new strain of the pathogenic microorganism Drechslera monoceras is MH1110.
No. 34 (FERM BP-3869), which contains the new strain of claim 8, which is a weed control agent. 14. The plant does not show pathogenicity and has a temperature of 15 degrees Celsius.
Dre-chslera (Dre-chslera), a pathogenic microorganism that is pathogenic to the genus Echinocloa spp.
monoceras) 1 or 2 new strains and 1 chemical herbicide
A weed control composition comprising at least one species. 15. The weed control composition according to claim 14, wherein the chemical herbicide is a diphenyl ether herbicide. 16. The diphenyl ether herbicide is CNP.
(Chemical name: 2,4,6-trichlorophenyl-4-nitrophenyl ether), or clomethoxynil (Chemical name: 2,4-dichlorophenyl-4-nitro-3-methoxyphenyl ether), or biphenox (Chemical name) And 2,4-dichlorophenyl-3-methoxycarbonyl-4-nyl ether). 16. The weed control composition according to claim 15, wherein 17. The weed control composition according to claim 14, wherein the chemical herbicide is an anilide herbicide. 18. The anilide herbicide is mefenacet (chemical name: 2-benzothiazol-2-yloxy-N).
-Methylacetanilide), or pretilachlor (chemical name; 2-chloro-2'6'-diethyl-N- (n-propoxyethyl) acetanilide), or naproanilide (chemical name; 2- (2-naphthoxy) propionic acid) Anilide) or NSK-850 (chemical name; N- [27-
(3′-methoxy) thienylmethyl-N-chloroaceto-2,6-dimethylanilide), or butachlor (chemical name; 2-chloro-2′6′-diethyl-N- (butoxymethyl) acetanilide, or Propanyl (chemical name: 3'4'-dichloropropionanilide) or clomeprop (chemical name: 2- (2,4-dichloro-3-)
Methylphenoxy) propionanilide) 18. The weed control composition according to claim 17, which is characterized in that 19. The weed control composition according to claim 14, wherein the chemical herbicide is a thiol carbamate herbicide. 20. A thiolcarbamate herbicide is Bentocarb (chemical name: S- (4-chlorobenzyl)-
N, N-diethyl thiol carbonate) or dimepiperate (chemical name; S- (1-methyl-1-phenethyl) -piperidine-1-carbathioate) or molinate (chemical name: S-ethyl- N, N-hexamethylene thiol carbamate) or esprocarb (chemical name: S-benzyl-N-ethyl-N- (1,2-dimethylpropyl) thiol carbamate). The weed control composition according to claim 19. 21. The weed control composition according to claim 14, wherein the chemical herbicide is a diazine herbicide. 22. The diazine herbicide is oxadiazone (chemical name: 5-ter-butyl-3- (2,4-dichloro-5-isopropoxyphenyl) -1,3,4-oxazole-2- (3H)-. On, or pyrazolate (chemical name; 4- (2,4-dichlorobenzoyl) -1,3-
Dimethyl-1H-pyrazol-5-yl-p-toluenesulfonate) 22. The weed control composition according to claim 21. 23. The weed control composition according to claim 14, wherein the chemical herbicide is a sulfonylurea herbicide. 24. A sulfonylurea herbicide is bensulfuron-methyl (chemical name: methyl 2- [3- (4,6-
Dimethylpyrimidin-2-yl) ureidosulfonylmethyl] benzoate) or pyrazosulfuron-ethyl (chemical name; ethyl-5- [3- (4,6-dimethoxypyrimidin-2-yl) ureidosulfonyl] -1-
24. The weed control composition according to claim 23, which is methylpyrazole-4-carboxylate. 25. The weed control composition according to claim 14, wherein the chemical herbicide is a triazine herbicide. 26. The method according to claim 25, wherein the triazine herbicide is cimetrin (chemical name: 2,4-bis (ethylamino) -6-methylthio-1,3,5-triazine). Weed control composition.