JPH06329513A - Drechslera monoceras veriant, weed-controlling agent with the same as active ingredient and method for controlling weed using the variant - Google Patents

Abstract

PURPOSE:To provide a microbial herbicide for Echinochloa crus-galli, alternative to chemical herbicides or capable of reducing application of such chemical herbicides. CONSTITUTION:The objective weed-controlling agent containing, as herbicidal active ingredient, Drechslera monoceras var. microsporas, a new variant classified as Drechslera monoceras having no adverse effects on cultivated plants such as rice and giving herbicidal activity on any kind of Echinochloa weeds including Echinochloa crus-galli even at temperatures of as low as 15 deg.C.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a variety (var .: variety) of Drechslera monoceras, which is not pathogenic to crops but pathogenic to Novier.
Monoceras var. Micro Porus (Drechsl
era monoceras var. microsp
orus), a weed control agent containing the variety as an active ingredient, and a weed control method using the variety.

[0002]

2. Description of the Related Art Recently, there has been a serious problem such as environmental pollution due to excessive use of organic synthetic pesticides and reduction of control effect due to emergence of pests and weeds having acquired drug resistance. In the mass media, the effects of chemical pesticides on the human body and the natural environment have been widely covered, interest in biopesticides has increased, and research and development of biopesticides have become active. However, the pesticide-free cultivation method and the biological control method are still in the midst of research, and it is a fact that a stable control effect cannot be obtained by their single application and sufficient crop production cannot be expected. In any case, reducing the application amount of the synthetic organic pesticide is very significant from the viewpoint of safety.

In the field of herbicides, research and development of microbial herbicides utilizing pathogenic microorganisms for weeds have been conducted mainly in the United States, and so far, phytophyta, a pathogenic microorganism of Stranglelevine (Morrenia odorate), which is a weed of the potato family, has been developed. Genus Palmibora (Phytophthora palimivol)
a) using Devine (trade name of Abbott), pathogenic microorganisms of the legume weed Northern Joint Vetch (Aeschynomene virginica), Corretotricum genus Glooeospolioides (Colletorichum gloeos)
Collego (trade name of Ecogen Co., Ltd.) using porioides) is on the market. However, no microbial herbicide has been put into practical use for the main crops such as wheat, rice, soybean and the like, which is a major weed in the genus Vime.

The wild species of Nobie are known as weeds in the rice-growing regions of the world, and have long been a problem as a typical paddy weed in Japan. It is said that the outbreak of fly caused the rice planting work in paddy fields. Yabino (Weeds Research (1975) vol. 2
0) according to the Echinochlo spp.
a oryzicola), Echinochloa colona, Echinochloa pyramidalis, Echinochloa stagnina, Echinochloa cruloa echlochloe quince, Echinochloa haplocla and Echinochloa haplocla. ) Exists, and is further classified into three varieties of the Crusagari species, namely, Formocensis (Himetainubie), Cruzgari (Innervier), and Platycola (Himeniubier). Responses to herbicides and pathogens differ between such varieties. The naturally occurring weed Novie is considered to be a hybrid of various species of the genus Echinochloa, and as a herbicide having a practical control effect, it must have a herbicidal action on all species of the genus Echinochloa. Further, even if it does not show a herbicidal action on all species of the genus Echinochloa, it is required to have at least a broad herbicidal effect spectrum for the species that are practically problematic to the genus Echinochloa.

Weeds of the genus Echinochloa have an ambient temperature of 10 ° C.
When it exceeds, it germinates and begins to grow. In order to carry out more effective control, it is preferable to act the control agent according to the germination time of Echinochloa. For example, in the paddy fields in Japan, the water temperature rises to about 10 ° C to 15 ° C.
~ June is the season for pest control.

Therefore, it is more preferable that the control agent for the genus Echinochloa has a broad herbicidal effect spectrum against the above-mentioned various species of the genus Echinochloa, and that a good effect can be obtained even at a low temperature of about 15 ° C.

Drechslera from the genus Echinochloa
ATCC-24461 (American as monoceras sp.
Type Culture Collection (ATCC); Catalgue of ATCC,
page 144) and IFO-9619 and IFO-980
0 (Fermentation Research Institute, Osaka (IFO) List of Culture, pag
e 177) is described. However, as described below, these strains do not show any herbicidal activity against the genus Echinochloa, and their esterase zymogram is different from that of the present invention.

[0008] Further, Drec showing pathogenicity to the genus Echinochloa
As hslera monoceras sp., JP-A-3-219883
MH-9011 (FERM), which has the highest effect at 25 ° C.
11 strains represented by BP-3416) are described. However, as will be described later, these strains have different esterase zymograms from those of the present invention,
The herbicidal activity against the genus Echinochloa hardly shows herbicidal activity at the germination temperature of the genus Echinochloa of about 15 ° C.

Therefore, in order to more effectively control Echinochloa genus with a microbial herbicide, it is required to obtain a microorganism having a sufficient Echinochloa genus control action even at a low temperature of about 15 ° C.

Furthermore, in paddy fields and upland fields, many weeds compete with crops in addition to Nobie, and the control of these weeds is also essential in practical use. At present, it is common to use several kinds of chemical herbicides as a mixture in order to control many of these weeds at once, but it is not possible to apply many kinds of chemical herbicides in large amounts. As described above, it is not preferable because it causes the emergence of drug-resistant weeds and pests and the problem of environmental pollution.

[0011]

SUMMARY OF THE INVENTION The present invention has been made for the purpose of solving the above-mentioned problems in the prior art. An object of the present invention is to provide a weed control agent and a weed control method that avoid environmental pollution and the appearance of resistant weeds and enable practical control. In other words, strains of the genus Drexlera that are pathogenic only to Nobie are infected with all species of the genus Echinochloa even at 15 ° C or lower, and a strain having a herbicidal activity is isolated from nature, and the viable cells are used as active ingredients. An object of the present invention is to provide a weed-controlling agent contained as.

[0012] Furthermore, by combining a new herbicide with a new variety of the genus Dorexella which shows pathogenicity only to Nobie and infects even at 15 ° C or lower, the application rate of the chemical herbicide is reduced, and the safety is improved. It is an object of the present invention to provide a sufficiently practical weed control agent.

Another object of the present invention is to provide a method for controlling weeds by using the above-mentioned new varieties of the genus Dolexlera in combination with a chemical herbicide, if necessary.

[0014]

Means for Solving the Problems The filamentous fungus according to the present invention is
Taxonomically Drechslera monceras
oceras), but is a variant that is clearly different from the previously known Dorex Rera monoceras. In the present application, the conventional monoceras species is referred to as Drexlera monoceras var. Monoceras (Drechslera m
onoceras var.monoceras), and the new variant according to the present invention was prepared according to Drexrella monoceras var. These are distinguished as Microsporus (Drechslera monoceras var. Microsporus).

The new variety according to the present invention was found to be pathogenic to all species of the genus Echinochloa, which is a weed nobie, even at 15 ° C. or lower, and a practical herbicidal action against nobie including the rice planting time was shown. Furthermore, it was confirmed to be extremely safe for cultivated plants such as rice. The new variety according to the present invention is selected from microorganisms in the natural world, and is safe without fear of environmental pollution, which is a concern with organic synthetic pesticides.

Further, the mixed composition of the new varieties of the genus Dreyxella and the chemical herbicides according to the present invention shows a sufficient controlling effect at the herbicidal application rate which cannot be controlled by each of them alone. The application rate can be reduced. Furthermore, due to the synergistic effect of mixing the new varieties of the genus Dolexlera with the chemical herbicides, the amount of conidia of the bacterium of the genus Dolexlera to be applied can be reduced and the production cost can be reduced.

The new varieties, weed control agents and weed control agents according to the present invention not only contribute to crop production but also contribute to avoiding environmental pollution and the emergence of drug-resistant weeds, which have become a problem in recent years.

The inventors of the present invention searched various pathogenic microorganisms for the purpose of controlling the important weeds in paddy fields and upland fields, and as a result of searching various pathogenic microorganisms, they showed herbicidal activity even at 15 ° C. or below. We found a novel strain of the genus Dolex Rera that has no pathogenicity to useful plants such as. As a result of various examinations, it was found that this bacterium is distinguished as a new variety which is clearly different from the conventional Dolex Rera monoceras. As mentioned above, this new variant was prepared using the conventional Drexlera monoceras var. In contrast to Monoceras, Drex Rera Monoceras va
r. The present invention was completed by distinguishing these as microsporus.

Further, by mixing the new variant of the present invention of the genus Dolexlera showing a selective pathogenicity against Nobie and an existing chemical herbicide, a remarkable synergistic effect is exhibited, which is 1/10 to 100 of the conventional one. A chemical herbicide with a 1-fold application rate can provide a sufficient controlling effect against Nobie.

The Nobye, which is the subject of the present invention, is a weed belonging to the genus Echinochloa of the family Gramineae, and specifically, it is Echinochloa oryzicola or Echinochloea.
nochloa crus-galli var. formosensis), barnyard grass (Ec
hinichloa crus-galli var.crus-galli), Echinochloa crus-galli var. praticola), Echino
chloa colona, Echinochloa pyramidalis, Echinochlo
a stagnina, Echinochloa haploclada, etc.

That is, the new varieties of the present invention include cultivated plants, rice, barley, wheat, rye, oats,
For maize, sorghum, millet etc. and grass, orchardgrass, Italian ryegrass, perennial ryegrass, sweet vernalgrass, tall fescue, medfescue etc. without showing any pathogenicity against Echinochloa genus Orijicola species, Echinochloa crusgalli species, Echinochloa corona species etc. Show pathogenicity.

The new variety of the present invention is a subject of the genus Echinochloa, which is a weed genus Nobie, determined by purely isolating the strain from the naturally occurring Nobie, and examining the pathogenicity of the strain from the strains obtained. It was obtained by selecting those that have strong pathogenicity against all the above-mentioned species and show no pathogenicity to rice. The selected strains were identified based on conidia morphology, gene analysis, and esterase isozyme pattern analysis. As a result, they were identified as new variants of Dorexella monoceras var. Differentiated from Monoceras, Drex Rera Monoceras var. It was microporous. For the morphology of conidia, see M. B. Ell
It was analyzed by the conventional method described below, which was established by is.

The new variety strain of the present invention can be used alone or in combination of two or more thereof as an active ingredient of a weed control agent. The method of using the new variety according to the present invention as a weed control agent is a method of directly using the viable cells obtained by culturing, a method of using the filtrate after culturing the cells, or a method of using both as a mixture. Etc. Furthermore, the new variety according to the present invention is a case where conidia or mycelia obtained by culturing in a nutrient medium are suspended in an aqueous solution containing a surfactant and the like, and the new variety is used alone or does not exhibit antagonisticity. It may be used as a mixture with chemical pesticides such as herbicides, fungicides and insecticides. When the new variety according to the present invention is used as a weed control agent, conidia having higher durability than hyphae are more desirable. The bacterial growth of the new variant according to the present invention can be performed using either a liquid medium or a solid medium, but the bacterial cells are proliferated by inoculating the bacterial cells into a liquid medium such as potato dextrose. Conidia are formed by crushing the body and then drying. In the case of a solid medium, conidia formation is promoted by inoculating a potato-dextrose agar medium or the like, removing the growing aerial hyphae and then drying.

As described above, the new variety according to the present invention enables large-scale culturing of mycelia such as conidia and hyphae, and can be industrially used as a weed control agent. Shows herbicidal action only on the weeds, such as by treating the rice at the time of transplanting rice into paddy fields,
Non-pathogenic and selective for cultivated plants, rice, barley, wheat, rye, oats, corn, sorghum, millet, grass, orchardgrass, Italian ryegrass, perennial ryegrass, sweet vernalgrass, tall fescue, medfescue, etc. A herbicidal effect can be obtained.

As a representative strain of the new variant of the present invention, Drec
hslera sp. MH-11110, MH-12102
4, MH-121025, MH-122124, MH-
122754, MH-122755 and MH-1227
56 strains can be mentioned. These strains have been deposited under the Budapest Treaty with the following deposit numbers and deposit dates at the Institute of Biotechnology, Institute of Industrial Science and Technology, Ministry of International Trade and Industry (renamed from the former Institute of Microbial Engineering and Technology on January 1, 1993). Has been done. The most significant feature of the new variant of the present invention is the esterase isozyme pattern shown in FIG.
For example, hyphal growth is observed even at a low temperature of about 10 ° C. Therefore, strains classified into the monoceras species of the genus Dolex Rera and having these characteristic points are included in the present invention.

The new variety according to the present invention is not affected by biological effects such as mycelial elongation inhibition and spore germination inhibition by some chemical herbicides, and when mixed, the herbicidal action against Nobie is dramatically improved. .

As a result of further studies on various herbicides, one or more strains of the new variant of the present invention and chemical herbicides such as oxadiazone, dimetamethrin, cimetrin, clomethoxynil, dimepiperate, trifluralin, naproanilide, paraquat, pyrazoxyphene,
Pyrazolate, butachlor, pretilachlor, bench ocarb, mefenacet, molinate, CNP, D
BN, MCP, promethrin, benzophenap, propanil, NSK-850, HW-52, cromeprop, esprocarb, bifenox, quinclorac, bromobutide, bensulfuron-methyl, pyrazosulfuron-ethyl, 2,4D, etc. The mixed weed control agents enable the control of nobies at application rates that cannot be controlled by conventional chemical herbicides alone.

Regarding the mechanism of action, absorption and transfer of chemical herbicides are enhanced due to invasion marks in plant tissues caused by infection with D. rexella, and similarly, plant tissues damaged by chemical herbicides. It is surmised that infection with the bacterium of the genus Dolex Rera has become easier. Reducing the application rate of chemical herbicides through the synergistic action of these Drexella spp. And chemical herbicides improves recent problems such as environmental pollution and the emergence of resistance, and not only agricultural producers but also general consumers. There is a big advantage for me.

In order to obtain a weed-controlling agent using the new variety of the present invention, the bacterial cells of this new variety may be used as they are together with the chemical herbicide bulk, if necessary. , A substance of a bacterium of the genus Dolexrella and an active ingredient of a chemical herbicide, if necessary, are mixed with an inert solid carrier or liquid carrier, and granules, flowables, wettable powders, emulsions, which are usually used in the form of preparations, It is desirable to prepare a liquid agent or the like.

The carrier is generally used for agricultural and horticultural chemicals, and may be solid or liquid as long as it is biologically inert, and is not limited to a particular carrier.

For example, as the solid carrier, clay, talc, bentonite, zeolite, calcium carbonate, diatomaceous earth, mineral powder such as white carbon, soybean powder, vegetable powder such as starch, polyvinyl alcohol, polyalkylene glycol, etc. Examples thereof include polymer compounds.
Examples of the liquid carrier include various organic solvents such as decane and dodecane, vegetable oil, mineral oil, water and the like.

The weed-controlling agent of the present invention has a content of the bacterium of the genus Dolexlera in the range of 10 ² to 10 ¹⁵ spores / g, preferably 10 ⁶ to 10 ¹² spores / g.

The content of the chemical herbicide in the weed control agent according to the present invention varies depending on the formulation form, but is usually 0.05 to 50% by weight for granules and 1 to 50 for flowable agents.
% By weight, 1-90% by weight for wettable powders, preferably 0.5-20% by weight for granules, 1 for flowable agents
It is 0 to 30% by weight, and the wettable powder is 10 to 50% by weight.
On the other hand, the content of the bacterium belonging to the genus Dolexrella is 10 ² to 10 ¹⁵ as spores, preferably 10 ⁶ to 10 ¹² per 1 g of the active ingredient of each composition.

As auxiliary agents, surfactants, binders, stabilizers and the like which are usually used in agricultural and horticultural chemicals can be used alone or in combination as required. As a stabilizer,
For example, using an antioxidant or a pH adjusting agent,
A light stabilizer is also used.

The content of the auxiliary agent is 0 to 80% by weight,
The content of the carrier is 100% by weight less the active ingredient and the content.

When the weed-controlling agent of the present invention is used in a field, the amount of conidia spores of a bacterium of the genus Dolex Rera is
10 ² to 10 ¹⁵ spores / 10 a, preferably 10 ⁶ to 1
It is 0 ¹² spore / 10a.

The weed-controlling agent according to the present invention is a novel microorganism, one or more of the new strains of the genus Dolerxella monoceras Microsporus and one or more of the following chemical weeds, or an antibacterial activity against the bacterium of the genus Dreyxella. Not only can it be used in combination with a fungicide, a pesticide, a pesticide such as a plant growth regulator, a fertilizer, a soil conditioner, etc., which does not have a salt, but it can also be mixed with these.

Examples of the herbicides that can be mixed include the following herbicides. 2,4-dichlorophenoxyacetic acid (2,4-D), 2-methyl-4-chlorophenoxyacetic acid (MCPA), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 2- ( 2,4-dichlorophenoxy) propionic acid (dichlorprop), 2-
(2-Methyl-4-chlorophenoxy) propionic acid (mecoprop), 2- (2,4,5-trichlorophenoxy) propionic acid (fenoprop), 4-
(2,4-dichlorophenoxy) butyric acid (2,4-D
B), 4- (2-methyl-4-chlorophenoxy) butyric acid (MCPB), 2- (2-naphthoxy) propionanilide (naproanilide), 2- (1-naphthoxy) N, N-diethylpropionamide (napro)
pamid), (+)-2- [4- (2,4-dichlorophenoxy) phenoxy] propionic acid methyl ester (diclofp-methyl), 2- [4- (5
-Trifluoromethyl-2-pyridyloxy) phenoxy] propionic acid butyl ester (fluazifo)
p), 2- [4- (3-chloro-5-trifluoromethyl-2-pyridyloxy) phenoxy] propionic acid methyl ester (haloxyfop), 2- [4-
(3,5-Dichloro-2-pyridyloxy) phenoxy] propionic acid-2-propynyl ester (chlo
razifop-propynyl), 2- [4- (6
-Chloro-2-quinoxanyloxy) phenoxy] propionic acid ethyl ester (quizalofop-et
hyl), 2-[-4- (6-chloro-2-benzoxazolyl) phenoxy] propionic acid ethyl ester (fenoxaprop-ethyl), 2- [4-
(6-Chloro-2-benzothiazolyloxy) phenoxy] propionic acid ethyl ester (fenthiapr
op-ethyl), 2,3,6-trichlorobenzoic acid (2,3,6-TBA), 3,6-dichloro-2-methoxybenzoic acid (dicamba)
a), 2,5-dichloro-3-aminobenzoic acid (amiben), 3,5,6-trichloro-2-
Methoxybenzoic acid (tricamba),
4-chloro-2,2-dimethyl barrel anilide (mon
alide), 3,4-dichloropropionanilide (propanil), 3,4-dichloro-2-methylacrylanilide (dicryl), 3,4-dichlorocyclopropanecarboxyanilide (cypromi)
d), 3,4-dichloro-2-methyl-pentaneanilide (karsil), 3-chloro-2,4-dimethylpentaneanilide (solan), N- (1,1-dimethylpropynyl) -3,5-dichloro Benzamide (pr
opzamide), N, N-dimethyl-2,2-diphenylacetamide (diphenamide), N
-Naphthylphthalamic acid (Naptalam), N-
(1,1-dimethylbenzyl) -2-bromo-ter
t. -Butylacetamide (buromobutid
e), 2-benzothiazol-2-yloxy-N-methylacetanilide (mefenasate), N-
[3- (1-Ethyl-1-methylpropyl) -5-isoxazolyl] -2,6-dimethoxybenzenamide (b
enzamizole), 1,1-dimethyl-3-phenylurea (fenuron), 3- (4-chlorophenyl) -1,1-dimethylurea (monuron),
3- (4-chlorophenyl) -2,1,1-trimethylisourea (trimeturon), 3- (4-chlorophenyl) -1-methoxy-1-methylurea (mo
nolinuron), 3- (4-chlorophenyl)-
1-methyl-1- (1-methylpropyn-2-yl) urea, 3- (4-bromophenyl)
-1-Methoxy-1-methylurea (metobrom
uron), 1- (2-methylcyclohexyl) -3-
Phenylurea (siduron), 1,1-dimethyl-3- (3-trifluoromethylphenyl) urea (f
llumeturon), 3- (3,4-dichlorophenyl) -1,1-dimethylurea (diuron),
3- (3,4-dichlorophenyl) -1-methoxy-1
-Methylurea (linuron), 3- (3,4-dichlorophenyl) -1-n-butyl-1-methylurea (neburon), 3- (3-chloro-4-methoxyphenyl) -1,1-dimethylurea (methoxur)
on), 3- (4-bromo-3-chlorophenyl) -1
-Methoxy-1-methylurea (chlorbromu
ron), 1- (4-difluorochloromethylmercapto-3-chlorophenyl) -3,3-dimethylurea, 3- (3-chloro-4-).
Methylphenyl) -1,1-dimethylurea (chlo
rtoluron), 3- [4- (4-chlorophenoxy) phenyl] -1,1-dimethylurea (chlor)
oxuron), 3- [4- (4-methoxyphenoxy) phenyl] -1,1-dimethylurea (difen)
oxuron), 3- [3- (N-tert.-butylcarbamoyloxy) phenyl] -1,1-dimethylurea, 3-benzoyl-3.
-(3,4-Dichlorophenyl) -1,1-dimethylurea, 1- (α, α-
Dimethylbenzyl) -3- (4-methylphenyl) urea (dymron), 3- (4-isopropylphenyl) -1,1-dimethylurea (isoproturo)
n), 3- (2-benzothiazolyl) -1,3-dimethylurea (methabenz thiazuron),
3- (2-benzothiazolyl) -1-methylurea (b
enzthiazon), 3- (hexahydro-
4,7-Methanoindan-5-yl) -1,1-dimethylurenor, 3-cyclooctyl-
1,1-dimethylurea, 1,3
-Dimethyl-3- (5-trifluoromethyl-1,3,3
4-thiadiazol-2-yl) urea (thiazf
luron), 1- (5-ethylsulfonyl-1,3,3
4-thiadiazol-2-yl) -1,3-dimethylurea (sulfodiazol), 3- [5- (1,1
-Dimethylethyl) -1,3,4-thiadiazole-2
-Yl] -1,3-dimethylurea (tebuthiu)
ron), 3- (5-tert.-butylisoxazol-3-yl) -1,1-dimethylurea (isou)
ron), 4- [2-chloro-4- (3,3-dimethylureido) phenyl] -2-tert. -Butyl-1,
3,4-oxadiazolin-5-one (dimfur
on), 3- (5-tert.-butyl-1,3,4-
Thiadiazol-2-yl) -4-hydroxy-1-
Methyl-2-imidazolidone (buthidazol)
e), 2-chloro-4,6-bis (ethylamino)-
1,3,5-triazine, 2-chloro-4-ethylamino-6-isopropylamino-
1,3,5-triazine, 2-chloro-4,6-bis (isopropylamino) -1,3
5-triazine, 2-chloro-
4-diethylamino-6-ethylamino-1,3,5-
Triazine, 2-chloro-4
-Ethylamino-6-tert. -Butylamino-1,
3,5-triazine
e), 2- (2-chloro-4-ethylamino-1,3,3
5-triazin-6-ylamino) -2-methylpropionitrile (cyanazine), 2-chloro-4-
Cyclopropylamino-6-isopropylamino-1,
3,5-triazine (prefox), 2- [2-chloro-4- (cyclopropylamino) -1,3,5-triazin-6-ylamino] -2-methylpropionitrile (procyazin), 2-methoxy -4-se
c. -Butylamino-6-ethylamino-1,3,5-
Triazine, 2-methoxy-
4,6-bis (isopropylamino) -1,3,5-triazine (promethon), 2-methylthio-4,
6-bis (ethylamino) -1,3,5-triazine (simetryne), 2-methylthio-4,6-bis (isopropylamino) -1,3,5-triazine (prometryne), 2-methylthio-4- Methylamino-6-isopropylamino-1,3,5-triazine (ametrine), 2-methylthio-4-ethylamino-6-tert. -Butylamino-1,3,3
5-triazine, 2-methylthio-4-isopropylamino-6- (3-methoxypropylamino) -1,3,5-triazine (meth)
protryne), 2-methylthio-4- (1,2-
Dimethylpropyl) -6-ethylamino-1,3,5-
Triazine, 2-methylthio-4-isopropylamino-6-methylamino-1,3,5-triazine (desmetryne),
4-amino-6-tert. -Butyl-3-methylthio-1,2,4-triazin-5 (4H) -one (met
ribuzin), 2-methylthio-4,6-bis (isopropylamino) -1,3,5-triazine (dip
Ropetryn), 2-tert. -Butylamino-
4-ethylamino-6-methoxyamino-1,3,5-
Triazine, 2-azido-4
-Isopropylamino-6-methylthio-1,3,5-
Triazine, 4-amino-
3-Methyl-6-phenyl-1,2,4-triazine-
5 (4H) -on (metamitron), 6-te
rt. -Butyl-4-isobutylideneamino-1,2,
4-triazin-5 (4H) -one (isomethi
ozin), 3-cyclohexyl-6-dimethylamino-1-methyl-1,3,5-triazine-2,4- (1
H, 3H) -dione, ethyl-N- (4-chloro-6-ethylamino-1,3,5-
Triazin-2-yl) -aminoacetate (egli
nazine), ethyl-N- (4-chloro-6-isopropylamino-1,3,5-triazin-2-yl)
-Amino acetate (proglinazine), 2
-Chloro-N-isopropylacetanilide (prop
achlor), N-methoxymethyl-2 ′, 6′-diethyl-2-chloroacetanilide (alachlo)
r), 2-chloro-2 ′, 6′-diethyl-N- (butoxymethyl) acetanilide (butachlor),
2-chloro-2'-ethyl-6'-methyl-N- (2-
Methoxy-1-methylethyl) acetanilide (met
olachlor), N, N-diallyl-2-chloroacetamide, 2-chloro-
2 ', 6'-Dimethyl-N- (2-methoxyethyl) acetanilide, 2,6-
Dinitro-N, N-dipropyl-4-trifluoromethylaniline (trifluralin), N-butyl-
N-ethyl-2,6-dinitro-4-trifluoromethylaniline (benfluralin), 2,6-dinitro-N-propyl-N-cyclopropyl-4-trifluoromethylaniline (profluralin),
N, N-diethyl-2,4-dinitro-6-trifluoromethyl-m-phenylenediamine (dinitram)
in), 4-isopropyl-2,6-dinitro-N, N
-Dipropylaniline (isopropaline),
2,6-dinitro-N-sec. -Butyl-4-ter
t. -Butylaniline, 4- (methylsulfonyl) -2,6-dinitro-N, N-dipropylaniline (nitralin), 3,4-dimethyl-2,6-dinitro-N-1-ethylpropylaniline (Pendimethalin), 3,5-dinitro-
N, N-dipropylsulfanilamide (oryzal
in), N-ethyl-N- (2-methylallyl) -2,
6-dinitro-4- (trifluoromethyl) aniline (ethalfluralin), N, N-diethyl-
2,4-dinitro-6-trifluoromethyl-m-phenylenediamine, 2-chloro-N- (4-methoxy-6-methyl-1,3,5-triazin-2-yl-aminocarbonyl) Benzenesulfonamide, methyl-
2- [3- (4-methoxy-6-methyl-1,3,5-
Triazin-2-yl) ureidosulfonyl] benzoate, methyl-2- [3- (4,6-dimethylpyrimidine-2-
Ile) ureidosulfonyl] benzoate (sulfo
methylon-methyl), methyl 2- [3-
(4,6-Dimethoxypyrimidin-2-yl) ureidosulfonyl] benzoate (bensulfuro)
n), 3- (4,6-dimethytoxy-1,3,5-triazin-2-yl) -1- [2- (2-methoxyethoxy) phenylsulfonyl] urea (tinosulfuron),
N- (2-chloroimidazole [1,2-α] pyridin-3-ylsulfonyl) -N ′-(4,6-dimethoxy-2-pyrimidyl) urea (TH-913), 1H-pyrazole-5-sulfone Amido-N-[(4,6-dimethoxy-2-pyrimidyl) aminocarbonyl] -1-methyl-4- (2-methyl-2H-tetrazol-5-yl) urea (DPX-A8947), 1-[{ o- (cyclopropylcarbonyl) phenyl} sulfonyl] -3
-(4,6-Dimethoxy-2-pyrimidinyl) urea (AC-140), ethyl 2- [3- (4-chloro-6)
-Methoxypyrimidin-2-yl) ureidosulfonyl] benzoate (chlorinuron), 3-
[(4-Methoxy-6-methyl-1,3,5-triazin-2-yl) ureidosulfonyl] -2-thiophenecarboxylic acid (thiameturon), 3,7-dichloro-8-quinolinecarboxylic acid (quinchlora)
c), 3,6-dichloro-2-pyridinecarboxylic acid (c
lopyralid), α- (2-chlorophenyl)-
α- (4-chlorophenyl) -5-pyrimidinemethanol (fenarimol), S, S-dimethyl-2-
(Difluoromethyl) -4- (2-methylpropyl)-
6-trifluoromethyl) -3,5-pyridinedicarbothioate (MON-15100, or MON-15
126, 4-chloro-5- (methylamino) -2- (3
-(Trifluoromethyl) phenyl) -3 (2H) -pyridazinone, O, O-bis (1-methylethyl) -S- [2-[(phenylsulfonyl) amino] ethyl] sulfodithioate (bens)
Ulide), (+)-2- [4,5-dihydro-4-
Methyl-4- (1-methylethyl) -5-oxo-1H
-Imidazol-2-yl] -5-methyl-3-pyridinecarboxylic acid (imazamethapr), 3-
[5- (1,1-dimethylethyl) -3-isoxazolyl) -4-hydroxy-1-methyl-2-imidazolidione, 2- [1- (ethoxyimino) -butyl] -3-hydroxy -5- (2H-Tetrahydrothiopyran-3-yl) -2-cyclohexen-1-one (cyclodydim).

In particular, the weed-controlling agent according to the present invention has a single herbicidal effect when it is used in combination with a chemical herbicide for fly, for example, a diphenyl ether-based, anilide-based, or thiol carbamate-based chemical herbicide. When applied in, a synergistic effect, which is unthinkable, is obtained, and it is possible to control fly by a lower dose than expected.

Further, the weed-controlling agent according to the present invention is used in combination with a herbicide for broad-leaved weeds, for example, a chemical herbicide such as a sulfonylurea type or a triazine type, when the herbicidal effect is applied individually. It is possible to obtain an unprecedented synergistic effect, to reduce the dose of pathogenic microorganisms, which is one of the components of the present invention, and to reduce the dose of chemical herbicides. It has become possible to control all paddy field weeds.

[0041]

TEST EXAMPLES AND EXAMPLES Microsporus, which is a new variant of the monoceras species of the genus Dolex Rera according to the present invention, and a mixed composition of the new variant and a chemical herbicide will be specifically described below. It is not limited to.

[0042]

[Test Example 1] Separation / Selection Method and Identification of Microorganisms 1) Separation Method of Pathogenic Microorganisms The naturally occurring pathogens of Novier are collected to focus on lesions.
Surface sterilization was performed by preparing a tissue section of 0 to 20 mm, immersing it in a 70% ethyl alcohol aqueous solution for 1 to 2 seconds, and then immersing it in an aqueous sodium hypochlorite solution having an effective chlorine concentration of 2% for 10 minutes. The surface-sterilized lesion tissue was washed three times with sterile distilled water, placed on an agar medium, and then statically cultured for 72 hours in a thermostat at 10 ° C. After culturing, the hyphae tips of the grown filamentous fungi were subjected to single hyphae separation under a stereoscopic microscope and purely separated on a nutrient medium to obtain a surplus of 7,000 strains. Strains MH-11110 and MH-121 as representatives of the new variants of the present invention, which are novel microorganisms among them
024, MH-121025, MH-122124, M
H-122754, MH-122755, MH-122
The 756 strain was submitted as a new variant to the Mycological Society of Japan and deposited at the Institute of Biotechnology, as mentioned above. The isolated filamentous fungi were tested for pathogenicity to Nobie and safety to rice.

2) Assay of Pathogenicity of Isolated Microorganisms for Novier and Safety for Rice Nobie and rice (variety: Nipponbare) were aseptically grown in test tubes to prepare test materials. That is, surface sterilization was performed by soaking seeds of Nobie and rice in a 70% ethyl alcohol aqueous solution for 1 to 2 seconds and then in a sodium hypochlorite aqueous solution having an effective chlorine concentration of 2% for 10 minutes. The surface-sterilized seeds were washed three times with sterile distilled water, seeded in a previously sterilized medium in a test tube, and then raised in a chamber for growing plants up to the 1.5 leaf stage.

On the other hand, the isolated microorganism of the present invention is potato.
A flora disk prepared by performing plate culture on dextrose agar medium at 25 ° C. for 4 days and punching the outer edge of the flora with a sterilized cork borer having a diameter of 5 mm was used as an inoculum source.

The bacterial lawn disk was inoculated into a medium in a test tube in which Nove and rice were grown, and cultured at 15 ° C. for 10 days in a chamber for growing plants. The evaluation was made in the following four grades from-to +++. The results are shown in Table 1.

+++; Death ++; Remarkable growth inhibition +; Slight growth inhibition −; No effect. The strain MH-0003 is of the genus Drexlera (Dre).
chslera sp. ), MH-0007 strain is forma genus (Phoma sp.), MH-0011 strain is for fusarium genus (Fusarium sp.), MH-0.
015 strain, MH-9011 strain, ATCC-2464
1, IFO-9619 and IFO-9800 are all genus Drechslera.
erasvar. monoceras), both of which were tested for comparison.

[0047]

[Table 1] 3) Identification of Microorganisms Strains showing remarkable pathogenicity to the fly and no effect on rice were identified. As a result, MH-11110, MH-121024, M
H-121025, MH-122124, MH-122
The 754, MH-122755 and MH-122756 strains were plated on malt agar medium at 28 ° C. for 7 days, and the colony size reached 65 to 75 mm in diameter, showing irregular growth. The color of the colony is gray black,
Conidia have traces, the width is 17 to 27 (most 19-23) μm, and the length is 35 to 110 (most 70
The average value of the ratio of the major axis to the minor axis (length / width) was 6.1 or less (majority, 4.5 to 5.0).
The shape is a little straight. Most conidia have 4 to 6 septa, and the shape of the conidia stalk is straight.

On the other hand, the conventionally known Drexlera monoceras (Drexlera monoceras var.
In Monoceras), the size of conidia is 16 to 25 in width.
(Majority 17 to 20) μm, length 60 to 150 (majority 100 to 120) μm, ratio of major axis to minor axis (length / length
The average value of the width is 5.9 or more (the majority, 6.2 to 6.5).
Met. The shape is also slightly curved. Most of the partition walls of conidia are 4 to 10 (majority 5 to 7), and the shape of the conidia stalk is straight.

From the above characteristics, according to the classification of Ellis,
MH-11110, MH-121024, MH-12
1025, MH-122124, MH-122754,
MH-122755, MH-122756 strains are classified into the monoceras species of the genus Dolex Rera, but there are differences in the size and shape of conidia, the distribution range of the number of septa, and because of different pathogenicity, It can be seen that this is a new variety different from the conventional strain.

In addition, the new variant of the present invention is the conventional Dolex Rera monoceras var. In order to demonstrate that it differs from Monoceras, a comparison of the isozyme patterns of the enzymes was performed according to the method of Hunter et al., As shown below. As the enzyme, α-esterase, which is not easily affected by the environment, was used.

The new variant of the present invention and various strains for comparison were tested in a potato-sucrose medium at 25 ° C. under dark conditions.
After static culture for 7 to 10 days, a cell mat was obtained. The resulting bacterial cell mat was washed with distilled water and weighed, then -8
It was freeze-dried in a freezer at 0 ° C., and 1-1.5 times volume of 0.05 M Tris-hydrochloric acid buffer (pH 7.4) was added for crushing. The disrupted liquid is filtered, and the filtrate is
After centrifugation at rpm, the supernatant was used as a sample. The amount of protein in the sample was quantified by the Lowry method.

Using a large slap gel electrophoresis layer, a 10% acrylamide gel was prepared in the lower layer and a concentrated gel was prepared in the upper layer, and the sample obtained by the above method was used in an amount of 50 per well.
After overlaying so as to obtain a protein amount of μg, electrophoresis was performed in a running buffer at 30 mA for 2 hours. Electrophoresis operating conditions: Acrylamide gel 10% gel Concentrated gel A 12 ml 1.8 ml B 9 ml-C-3 ml D 140 μl 36 μl TEMD 20 μl 12 μl H ₂ O 15 ml 7.2 ml A: Acrylamide 29.8% BIS Acrylamide 0.2% B : 1.5 M Tris-HCl (pH 8.8) C: 0.5 M Tris-HCl (pH 6.8) D: 10% ammonium persulfate sample buffer 0.5 M Tris-HCl (pH 6.8) 5 ml 0.05% BPB 2 ml Glycerol 2 ml H ₂ O 28 ml running buffer 250 mM Tris 1.92 M glycine The gel subjected to electrophoresis was stained with esterase as follows. 0.05M Tris-hydrochloric acid, 2m in 100ml
1% α-naphthyl acetic acid / 50% acetone aqueous solution and 1
The resulting gel was dipped in a staining solution containing 00 mg of fast violet B salt, shaken, and stained for 30 minutes. After staining, the gel was washed with distilled water and the mobility of each band was measured. The zymogram pattern of this esterase is shown in FIGS. 1 and 2 for each strain. Figure 1
2 is a zymogram pattern of a new variant of the present invention, and FIG.
Is from the conventional Drechslera monoceras var.monoceras. From these results, it was suggested that the new variety of the present invention is clearly different from the conventional species of the genus Dolex Rera monoceras. The application of the esterase electrophoretic pattern used for classification of microorganisms used in this example is a method generally used for mycelia.

In addition, at the gene level, Drexrella monoceras var. Monoceras and New Variant of the Present Invention Drex Rera Monoceras var. It was expected to be different from microsporus, and indeed, RNA encoded by DNA could also be found among the variants.

From the above characteristics, the strain MH-111 of the present invention
010, MH-121024, MH-121025, M
H-122124, MH-122754, MH-122
755, MH-122756, etc. are Drex Rera monoceras var. It was determined to be a new variant different from Monoceras.

The new variant according to the present invention was determined not to be a human pathogen in the National Institute of Preventive Health's safety management rules for pathogens and the like.

On the other hand, MH-0003, which was not shown to be pathogenic to Novier, was a genus of Drechs.
Lera sp.), MH-0007 strain is a genus of Phoma (Phoma
sp.). The MH-0011 strain, which is pathogenic for Nobie and rice, is Fusarium s.
p.), MH-0015 and MH-9011 which are pathogenic to Nobie and show no pathogenicity to rice.
Are all known strains of the genus Dolerxella monoceras (Japanese Patent Laid-Open No. 3198883).

The above identification is based on MBEllis (1971) Demariace
us Hyphomycetes p.608, Commonwealth Mycological Ins
titute, Kew, England and MB Ellis (1976) More Demariaceu
s Hyphomycetes p.507, Commonwealth Mycological Inst
It was done with reference to itute, Kew, England.

[Test Example 2] Effect of controlling the fly by the bacterium of the genus Dolexrella The genus Dolexrella isolated from the natural world was inoculated on an oat mill agar medium, and static culture was carried out at 25 ° C for 7 days.
Thereafter, the aerial hyphae were removed with distilled water to promote the formation of conidia. The conidia obtained were 10 ³ and 1
0 of ⁵ / ml and made so 0.02% Triton X-10
0 (trade name / manufactured by Rohm and Haas Co.) was suspended in an aqueous solution to prepare a weed-controlling agent containing a bacterium of the genus Dreyxella.

On the other hand, seeds of Novier and rice (variety: Nipponbare) were sown in paddy soil filled in a pot of 1 / 10000a, and seedlings were raised to the 1.5 leaf stage. Water depth is about 5
cm of water, then 5 ml of the above weed control agent containing conidiospores of the genus Dreyxella is added dropwise, and day and night 1
After cultivating for 10 days in an artificial climate room at 5 ° C., the effect of Drexella spp. On Nobie and rice was tested in Test Example 1.
It evaluated by the same criteria as. The results are shown in Table 2.

+++; Death ++; Significant growth inhibition +; Slight growth inhibition −; No effect

[0061]

[Table 2] As a result of the test, MH- of the genus Dolex Rera according to the present invention
1111010, MH-121024, MH-12102
5, MH-122124, MH-122754, MH-
122755, MH-122756, under the low temperature condition of 15 ° C, MH-0015, MH-90 against Novie.
The herbicidal action was more than 1000 times better than that of No. 11, and the safety against rice was confirmed.

[Test Example 3] The pathogenicity of various microorganisms of the present invention to various Echinochloa species and rice was evaluated in the same manner as in 2) of Test Example 1. That is, as a novier, Echinochloa corona species, Echinochloa orijicola species (Japanese name: Tainubie), Echinochloa crus-galli variety Formosensis (Japanese name: Himetainubiae),
Varieties of Echinochloa crusgari varieties Kursugari (Japanese name: Inubier) and varieties of Echinocloa crusgari varieties Platycola (Japanese name: Himeinubiae) are cultivated as rice, Nipponbare (variety name), Sasaniki (variety name), Koshihikari (variety) Name) was tested. Ten days after the inoculation, the pathogenicity of the microorganisms of the present invention to the millet and rice was evaluated, and the results are shown in Table 3.

[0063]

[Table 3] +++; Death ++; Remarkable growth inhibition +; Slightly growth inhibition −; No effect As a result of the test, new variants of the genus Dolerxella monoceras according to the present invention, MH-11110, MH-12102
4, MH-121025, MH-122124, MH-
122754, MH-122755, MH-12275
The 6 strains showed strong herbicidal activity against all species and varieties of the genus Echinochloa, showed excellent herbicidal activity, and were further confirmed to be safe against rice.

[Test Example 4] Effect of chemical herbicide on various biological properties of Dorexella spp. [0064] A flora disk of Dorexella spp. Produced by the method shown in 2) of Test Example 1 was used as a chemical herbicide CNP. (A in the table), mefenacet (E in the table), pretilachlor (I in the table), benchocarb (L in the table), bensulfuron (P in the table) and the like were inoculated on potato-dextrose agar medium containing 500 ppm each. . After static culture was carried out at 25 ° C. for 5 days, the diameter of the formed flora was measured and used as mycelial extension. In addition, Triton X- of a conidial suspension of the bacterium of the genus Dreyxella prepared by the method shown in Test Example 2
After replacing 100 aqueous solutions with potato dextrose liquid medium containing 500 ppm of each chemical herbicide, 24 at 25 ° C.
The static culture was performed for an hour. After culturing, the presence or absence of conidiospore germination was observed under a microscope, and the conidiospore germination rate was calculated. The effect of each chemical herbicide on hyphal elongation and conidiospore germination rate of the bacterium of the genus Dolexlera was expressed as a percentage relative to the control group containing no chemical herbicide, and the results are shown in Table 4.

As shown in Tables 4-1 and 4-2, inhibition of hyphal elongation and conidiospore germination by chemical herbicides was hardly observed in the strains of the genus Dolexlera according to the present invention.

[0066]

[Table 4]

[0067]

[Table 5] [Test Example 5] Control effect of Nobie by a mixed composition of a bacterium of the genus Dolexlera and a chemical herbicide CNP The strain of the present invention, the genus Dolexlera monoceras sp. Microsporus var. The static culture was carried out for 7 days. After this,
The formation of conidia was promoted by removing the aerial hyphae with distilled water. The conidia thus obtained are suspended in a 0.02% Triton X-100 (trade name / produced by Rohm and Haas Co.) aqueous solution so as to have a predetermined concentration, and a bacterium belonging to the genus Dolex Rera is used as an active ingredient. A weed control agent was prepared. Also,
For CNP, 30% to 1 mg of 9% granule was weighed and used as a chemical herbicide.

On the other hand, seeds of Nobie and rice (variety: Nipponbare) were sown in paddy soil filled in a pot of 1 / 10000a, and seedlings were raised to the 1.5 leaf stage. Water depth is about 5
After the water was flooded to cm, 5 ml of the above weed control agent containing conidiospores of the genus Dreyxella was dropped, and at the same time, the chemical herbicide CNP was treated. After culturing for 10 days in an artificial climate room at 15 ° C. in the daytime at night, the effect of Drexella spp. And a chemical herbicide on Nobie and rice was evaluated according to the same criteria as in Test Example 1. The results are shown in Table 5. +++; Withered ++; Remarkable growth inhibition +; Slightly growth inhibition −; No effect Although the results in Table 5 are only one example, significant synergism was observed under any low temperature condition in any combination of the bacterium of the genus Dolexrella and the chemical herbicide. Showed action.

[0069]

[Table 6] Nobie control rate (0 to 100%), () indicates damage to rice, and (-) indicates that no damage was observed.

[Formulation Examples and Test Examples] Next, formulation examples and herbicidal activity test examples of the herbicide according to the present invention will be shown.

[Formulation Example-1] (Granule) 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite were well mixed. Then, 1 g of conidia of the genus Dolex Rera (MH-11110 strain) were granulated.
A spore suspension containing 10 ⁹ per spore was added for wetting and then extruded and granulated on a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-2] (Granule) 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite were well mixed. After that, 1 g of granules of conidia of the genus Dolex Rera (MH-121024 strain)
A spore suspension containing 10 ⁸ per spore was added for wetting, and then extrusion granulated with a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-3] (Granule) 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite were well mixed. Then, 1 g of conidiospores of the genus Dolex Rera (MH-121025 strain) were granulated.
A spore suspension containing 10 ⁹ per spore was added for wetting and then extruded and granulated on a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-4] (Granule) 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite were well mixed. Then, 1 g of conidiospores of the genus Dolex Rera (MH-122124 strain) were granulated.
A spore suspension containing 10 ⁸ per spore was added for wetting, and then extrusion granulated with a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-5] (Granule) 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite were well mixed. Then, 1 g of conidiospores of the genus Dolex Rera (MH-122754 strain) were granulated.
A spore suspension containing 10 ⁹ per spore was added for wetting and then extruded and granulated on a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example 6] (Granule) 2% by weight of Neoperex (trade name / manufactured by Kao), 2% by weight of Sun Extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp), and 96% by weight of zeolite were well mixed. Then, 1 g of granules of conidia of the genus Dolex Rera (MH-122755 strain)
A spore suspension containing 10 ⁸ per spore was added for wetting, and then extrusion granulated with a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-7] (Wettable powder) Neoperex (trade name / manufactured by Kao) 2% by weight, Triton (X-100) 2% by weight, white carbon 5% by weight
Was impregnated with a spore suspension containing 10 ¹⁰ conidiospores of the genus Dolex Rera (MH-122756 strain) per 1 g of the wettable powder, air-dried, and then 91% by weight of diatomaceous earth was well pulverized and mixed to obtain the wettable powder. Obtained.

[Formulation Example-8] (Wettable powder) Neoperex (trade name / manufactured by Kao; sodium dodecylbenzenesulfonate): 2% by weight, Neugen EA80
(Trade name, manufactured by Sanyo Kasei; polyoxyethylene nonylphenyl ether): 1% by weight, white carbon: 5% by weight, and diatomaceous earth 92% by weight.
H-11110 strain) 1 conidiospore per 1 g of wettable powder
After impregnation with a spore suspension containing 0 ⁹ cells and air-drying, the mixture was thoroughly pulverized and mixed to obtain a wettable powder.

[Formulation Example-9] (Wettable powder) Neoperex (trade name / manufactured by Kao) 2% by weight, Triton (X-100) 2% by weight, white carbon 5% by weight
Was impregnated with a spore suspension containing 10 ¹⁰ conidiospores of the genus Dolex Rera (MH-122756 strain) per 1 g of the wettable powder, air-dried, and then 91% by weight of diatomaceous earth was well pulverized and mixed to obtain the wettable powder. Obtained.

[Formulation Example-10] (Wettable powder) Neoperex (trade name / manufactured by Kao; sodium dodecylbenzenesulfonate): 2% by weight, Neugen EA80
(Trade name, manufactured by Sanyo Kasei; polyoxyethylene nonylphenyl ether): 1% by weight, white carbon: 5% by weight, and diatomaceous earth 92% by weight.
H-121024 strain) conidiospores per 1 g of wettable powder 1
After impregnation with a spore suspension containing 0 ⁹ cells and air-drying, the mixture was thoroughly pulverized and mixed to obtain a wettable powder.

[Formulation Example-11] (Wettable powder) Neoperex (trade name / manufactured by Kao; sodium dodecylbenzenesulfonate): 2% by weight, Neugen EA80
(Trade name, manufactured by Sanyo Kasei; polyoxyethylene nonylphenyl ether): 1% by weight, white carbon: 5% by weight, and diatomaceous earth 92% by weight.
H-121025) conidiospores per 1 g of wettable powder 1
After impregnation with a spore suspension containing 0 ⁹ cells and air-drying, the mixture was thoroughly pulverized and mixed to obtain a wettable powder.

[Formulation Example-12] (Flowable agent) 10% by weight of sun extract (P252) dissolved in 80% by weight of water was wet pulverized and mixed, and then a bacterium of the genus Dolex Rera (M
H-122754 strain) conidiospore flowable agent 1m
0.4 wt% of Kelzan S (trade name, manufactured by Kelco) dissolved in 9.6 wt% of a spore suspension containing 10 ¹⁰ cells per liter was added and mixed to obtain a flowable agent.

[Formulation Example-13] (Flowable agent) Sun extract P252 (trade name, dissolved in 70% by weight of water)
The same as the above): 10% by weight, and after further mixing 10% by weight of a spore suspension containing 10 ¹⁰ conidiospores of the genus Dolex Rera (MH-11110 strain) per ml of the flowable agent,
Kerzan S (trade name, manufactured by Kelco; xanthan gum) dissolved in 9.8% by weight of water after wet pulverization and mixing: 0.2
A wt% was added and mixed to obtain a flowable agent.

[Formulation Example-14] (Flowable agent) 10 wt% of the sun extract (P252) dissolved in 80 wt% of water was wet pulverized and mixed, and then a bacterium of the genus Dolex Rera (M
H-122756 strain) conidiospore flowable agent 1m
0.4 wt% of Kelzan S (trade name, manufactured by Kelco) dissolved in 9.6 wt% of a spore suspension containing 10 ¹⁰ cells per liter was added and mixed to obtain a flowable agent.

[Formulation Example-15] (Flowable agent) Sun extract P252 (trade name, dissolved in 70% by weight of water)
The same as the above): 10% by weight, and after further mixing 10% by weight of a spore suspension containing 10 ¹⁰ conidiospores of the genus Dolex Rera (MH-121024 strain) per ml of the flowable agent,
Kerzan S (trade name, manufactured by Kelco; xanthan gum) dissolved in 9.8% by weight of water after wet pulverization and mixing: 0.2
A wt% was added and mixed to obtain a flowable agent.

[Formulation Example-16] (Flowable agent) 10 wt% of sun extract (P252) dissolved in 80 wt% of water was wet-milled and mixed, and then a bacterium of the genus Dolex Rera (M
H-122124 strain) conidiospore flowable agent 1m
0.4 wt% of Kelzan S (trade name, manufactured by Kelco) dissolved in 9.6 wt% of a spore suspension containing 10 ¹⁰ cells per liter was added and mixed to obtain a flowable agent.

[Formulation Example-17] (Flowable agent) Sun extract P252 (trade name, dissolved in 70% by weight of water)
The same as the above): 10% by weight, and after further mixing 10% by weight of a spore suspension containing 10 ¹⁰ conidiospores of the genus Dolex Rera (MH-121025 strain) per 1 ml of the flowable agent,
Kerzan S (trade name, manufactured by Kelco; xanthan gum) dissolved in 9.8% by weight of water after wet pulverization and mixing: 0.2
A wt% was added and mixed to obtain a flowable agent.

[Formulation Example-18] (Dry flowable agent) Sodium alkylbenzene sulfonate: 15% by weight and polypropylene glycol polyethylene glycol ether: 85% by weight, and further a bacterium of the genus Dolex Rera (MH-
1111010 strain) conidiospores as a dry flowable agent 1
A dry flowable agent was obtained by mixing 10 ¹⁰ pieces per g.

[Formulation Example-19] (Dry flowable agent) Sodium alkylbenzene sulfonate: 15% by weight and polypropylene glycol polyethylene glycol ether: 85% by weight, and further a bacterium of the genus Dolex Rera (MH-
121024 strain) conidial dry flowable agent 1
A dry flowable agent was obtained by mixing 10 ¹⁰ pieces per g.

[Formulation Example-20] (Dry flowable agent) Sodium alkylbenzene sulfonate: 15% by weight and polypropylene glycol polyethylene glycol ether: 85% by weight, and further a bacterium of the genus Dolex Rera (MH-
122754 strains) conidiospores as a dry flowable agent 1
A dry flowable agent was obtained by mixing 10 ¹⁰ pieces per g.

[Formulation Example-21] (Dry flowable agent) Sodium alkylbenzene sulfonate: 15% by weight and polypropylene glycol polyethylene glycol ether: 85% by weight, and further a bacterium of the genus Dolex Rera (MH-
Contaminated spores of strain 122756)
A dry flowable agent was obtained by mixing 10 ¹⁰ pieces per g.

[Formulation Example-22] (Powder) Emulgen 910 (trade name, manufactured by Kao; polyoxyethylene nonylphenyl ether): 0.5% by weight and kaolin clay: 99.5% by weight were well pulverized and mixed, and further Drosophila spp. (MH-1110 strain) conidial spores were mixed at 10 ⁸ per 1 g of dust to obtain a dust.

[Formulation Example-23] (Emulsion) Lecithin: 5% by weight, heavy white oil 94% by weight
The conidiospores of the genus Dolex Rera (MH-121024 strain) were suspended in 10 ¹⁰ per 1 g of the emulsion, and an equal amount of 1% by weight of Triton X-100 was added and mixed to form an emulsion. .

[Formulation Example-24] (Granule) Finely ground chemical herbicide, 9% by weight of CNP (A in the table), 2% by weight of Neoperex (trade name / manufactured by Kao), Sun Extract P252 (commercial product) 2% by weight (manufactured by Sanyo Kokusaku Pulp) and 87% by weight of zeolite were mixed well, and then a spore suspension containing 10 ⁸ conidiospores of the genus Dolex Rera (MH-122754 strain) per 1 g of granules was added. Moisten,
Next, it was extruded and granulated with a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-25] (Granule) 3.5% by weight of a chemical herbicide, butachlor (in the table; H), 2% by weight of Neoperex (trade name / manufactured by Kao), Sun Extract P252 (trade name) (Manufactured by Sanyo Kokusaku Pulp Co., Ltd.) and 22.5% by weight of zeolite and 92.5% by weight of zeolite, and then a spore suspension containing 10 ⁸ conidiospores of the genus Dolex Rera (MH-121025 strain) per 1 g of granules was added. Wetted and then extruded and granulated on a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-26] (Granule) 4% by weight of finely ground chemical herbicide, mefenacet (E in the table), 2% by weight of Neoperex (trade name / manufactured by Kao), Sun Extract P252 (commodity) Name / Made by Sanyo Kokusaku Pulp)
After thoroughly mixing 2% by weight and 92% by weight of zeolite, a spore suspension containing 10 ⁸ conidiospores of the bacterium of the genus Dolex Rera (MH-121024 strain) per 1 g of granules was added to moisten it, and then small injection It was extruded and granulated by a molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-27] (Granule) A finely pulverized chemical herbicide, benzsulfuron (in the table; P).
0.2% by weight, NeoPerex (trade name / made by Kao) 2
Wt%, Sun extract P252 (trade name / manufactured by Sanyo Kokusaku Pulp) 2% by weight, and 95.8% by weight of zeolite were thoroughly mixed, and then a bacterium of the genus Dolex Rera (MH-11110 strain)
The spore suspension containing 10 ⁸ per 1 g of granules of the conidia was added and moistened, and then extruded and granulated by a small injection molding machine. After air-drying and crushing this, 0.3 with a granulator
~ 2 mm granules were obtained.

[Formulation Example-28] (Granule) 0.3% by weight of finely ground chemical herbicide, pyrazosulfuron-ethyl (in the table; Q), goshenol (Goseno)
1) GL-05s (Nippon Gosei Kagaku Co., Ltd. ^PVA ): 2% by weight, Sun Extract P-252 (Sanyo Kokusaku Pulp Co., Ltd. sodium ligninsulfonate): 2% by weight, and clay: 9
After thoroughly mixing 5.7% by weight, a spore suspension containing 10 ⁸ conidiospores of the genus Dolex Rera (MH-1110 strain) per 1 g of granules was added to moisten it, and then extruded by an injection molding machine. Granulated. This was air-dried and crushed, and then granulated with a granulator to 0.3 to 1 mm to obtain a granule.

[Formulation Example-29] (Granule) 1.5% by weight of finely ground chemical herbicide oxadiazone (D in the table), Gosenol GL-
05s (Nippon Gosei Kagaku Co., Ltd. ^PVA ): 2% by weight, Sun Extract P-252 (Sanyo Kokusaku Pulp Co., Ltd. sodium lignin sulfonate): 2% by weight, and clay: 94.5 parts by weight. , Drexrella spp. (MH-122
Conspores of strain 755) were moistened by adding a spore suspension containing 10 ⁸ per 1 g of granules, and then extruded and granulated by an injection molding machine. After air-drying and crushing this, 0.3 ~
Granules were obtained by granulating to 1 mm.

[Preparation Example-30] (Granule) 1. The finely ground chemical herbicide, cimetrin (in the table; S) was added to 1.
5% by weight, Gosenol GL-05
After thoroughly mixing s ( ^PVA, Nippon Synthetic Chemical ^Industry Co., Ltd.): 2% by weight, Sun Extract P-252 (sodium lignin sulfonate manufactured by Sanyo Kokusaku Pulp Co., Ltd.): 2% by weight and clay: 94.5% by weight , Drexrella spp. (MH-12275
Conidial spores of 6 strains) were added to a spore suspension containing 10 ⁸ per 1 g of granules to moisten it, and then extruded and granulated by an injection molding machine. After air-drying and crushing this, 0.3-1m with a granulator
Granulated into m to obtain granules.

[Formulation Example-31] (Granule) 6% by weight of a chemical herbicide, molinate (N in the table), Neoperex (trade name / manufactured by Kao) 2% by weight, Sun Extract P
252 (trade name / manufactured by Sanyo Kokusaku Pulp) and 2% by weight of zeolite and 90% by weight of zeolite were well mixed, and then a spore suspension containing 10 ⁸ conidiospores of the bacterium of the genus Dolexrella (MH-1110 strain) per 1 g of granules. Was added to wet and then extruded and granulated with a small injection molding machine. This was dried by air drying and crushed to obtain granules of 0.3 to 2 mm with a granulator.

[Formulation Example-32] (Wettable powder) Chemical herbicide, 30% by weight of chloromethoxynil (B in the table), 2% by weight of Neoperex (trade name / manufactured by Kao), Triton (X-100) 2% by weight and 5% by weight of white carbon were added to the genus Dolex Rera (MH-122124 strain)
The spore suspension containing 10 ⁹ per 1 g of wettable powder was impregnated with the conidia of Example 1 and then 61% by weight of air-dried diatomaceous earth was well pulverized and mixed to obtain a wettable powder.

[Formulation Example-33] (Wettable powder) Chemical herbicide, propanil (J in the table) 40% by weight, Neoperex (trade name / manufactured by Kao) 2% by weight, Triton (X)
-100) 2% by weight and 5% by weight of white carbon were impregnated with a spore suspension containing 10 ⁹ conidiospores of the genus Dreyxella (MH-121024 strain) per 1 g of wettable powder, and then air-dried diatomaceous earth 51 A wettable powder was obtained by thoroughly pulverizing and mixing the weight%.

[Formulation Example-34] (Wettable powder) Chemical herbicide, Benchocarb (in the table; L) 20% by weight,
Neoperex (product name / Kao) 2% by weight, Triton (X-100) 2% by weight, white carbon 5% by weight
Was impregnated with a spore suspension containing 10 ⁹ conidiospores of the genus Dolex Rera (MH-11010 strain) per 1 g of the wettable powder, and then 71% by weight of air-dried diatomaceous earth was well pulverized and mixed to obtain the wettable powder. Obtained.

[Formulation Example-35] (Wettable powder) 40 parts by weight of a chemical herbicide, naproanilide (in the table; F),
Neoperex (trade name / manufactured by Kao; sodium dodecylbenzene sulfonate): 2% by weight, Neugen EA80
(Commercial name, Sanyo Kasei; polyoxyethylene nonylphenyl ether): 1% by weight, white carbon: 5% by weight and diatomaceous earth 52% by weight, a bacterium of the genus Dolex Rera (M
H-122124 strain) conidiospores per gram of wettable powder 1
After impregnation with a spore suspension containing 0 ^{9 of} them, the mixture was thoroughly pulverized and mixed to obtain a wettable powder.

[Formulation Example-36] (Wettable powder) Chemical herbicide, dimepiperate (M in table) 20% by weight,
Sodium alkylbenzene sulfonate: 2% by weight, polyoxyethylene alkylphenyl ether: 1% by weight
And Sikrite: 77% by weight was impregnated with a spore suspension containing 10 ⁹ conidiospores of a bacterium of the genus Dreyxella (MH-122754 strain) per 1 g of wettable powder, and then pulverized and mixed well to give a wettable powder. Obtained.

[Formulation Example-37] (Wettable powder) Chemical herbicide, clomeprop (K in the table): 40% by weight, white carbon: 5% by weight, polyoxyethylene alkylphenyl ether sulfate ammonium salt: 6% by weight, Sodium lignin sulfonate: 2% by weight and diatomaceous earth: 47% by weight were well pulverized and mixed using a Jet-O-mizer, and a bacterium of the genus Dolex Rera (MH-121024).
The spore suspension containing 10 ⁹ per 1 g of the wettable powder was impregnated with conidiospores of the (strain) strain, and then thoroughly pulverized and mixed to obtain a wettable powder.

[Preparation Example-38] (Flowable Agent) Finely ground chemical herbicide, CNP (in table; A) 45% by weight
And sun extract dissolved in 35% by weight of water (P252)
10 wt% was wet pulverized and mixed, and then Kerzan S was dissolved in 9.6 wt% of a spore suspension containing 10 ⁹ conidiospores of the genus Dolex Rera (MH-1110 strain) per 1 ml of the flowable agent (trade name, Kelco) 0.4% by weight
Was added and mixed to obtain a flowable agent.

[Formulation Example-39] (Flowable agent) Chemical herbicide, pretilachlor (in the table; I) 10% by weight
And sun extract dissolved in 70% by weight of water (P252)
10 wt% was wet pulverized and mixed, and then Kerzan S was dissolved in 9.6 wt% of a spore suspension containing 10 ⁹ conidiospores of the genus Dolex Rera (MH-122754 strain) per 1 ml of the flowable agent (trade name, Kelco) 0.4% by weight
Was added and mixed to obtain a flowable agent.

[Formulation Example-40] (Flowable agent) Chemical herbicide, Benchocarb (in the table; L) 30% by weight,
Sodium lignin sulfonate: 2% by weight, xanthan gum: 0.3% by weight, and polyoxyethylene alkylaryl ether: 1% by weight, to which 56.7% by weight of water was added to further add a bacterium of the genus Drexrella (MH-121024 strain). 10% by weight of a spore suspension containing 10 ⁹ conidia per ml of the flowable agent was mixed, and then finely ground using a sand grinder to obtain a flowable agent.

[Formulation Example-41] (Flowable Agent) Chemical herbicide, sun extract P252 (trade name, dissolved in 30% by weight of Esprocurve (O in the table; O) and 40% by weight of water (trade name,
The same as the above): 10% by weight, and after further mixing 10% by weight of a spore suspension containing 10 ⁹ conidiospores of the genus Dolex Rera (MH-122756 strain) per ml of the flowable agent,
Kerzan S (trade name, made by Kelco; xanthan gum) dissolved in 9.6% by weight of water after wet pulverization and mixing: 0.4
% By weight was added and mixed to obtain a flowable agent.

[Formulation Example-42] (Dry flowable agent) A finely ground chemical herbicide, pyrazolate (in the table; R) 60
% By weight, sodium alkylbenzene sulfonate: 5% by weight and polypropylene glycol polyethylene glycol ether: 35% by weight, and conidiospores of a bacterium of the genus Dolexrella (MH-1110 strain) are chemically herbicides 1.
A dry flowable agent was obtained by mixing 10 ⁹ per g.

[Formulation Example-43] (Powder) Finely ground chemical herbicide, bensulfuron (in the table; P)
0.2% by weight, Emulgen 910 (trade name, manufactured by Kao: polyoxyethylene nonylphenyl ether): 0.5% by weight and kaolin clay: 99.3% by weight were well pulverized and mixed, and further, a bacterium of the genus Dolex Rera (MH- 1111010
Conidiospores of (strain) were mixed at a rate of 10 ⁸ per 1 g of powder to obtain a powder.

[Formulation Example-44] (Dust) Finely ground chemical herbicide, mefenacet (in the table; E) 4
% By weight, Emulgen 910 (trade name, manufactured by Kao: polyoxyethylene nonylphenyl ether): 1% by weight, sodium lignin sulfonate: 3% by weight, polyoxyethylene alkylaryl ether: 2% by weight and clay:
90% by weight is mixed and pulverized, and the bacterium of the genus Dolex Rera (M
H-121024 strain) 10 conidiospores / g powder
⁹ pieces were mixed to obtain a powder.

[Formulation Example-45] (Emulsion) Chemical herbicide, bensulfuron (P; in the table; 1% by weight), lecithin: 5% by weight, heavy white oil 94% by weight, and Drexrella spp. (MH-11110 strain) 10 ^{9 of the} conidia of the above were suspended in 1 ml of the emulsion, and an equal amount of 1% by weight of Triton X-100 was added and mixed to emulsify to obtain an emulsion.

[Preparation Example-46] (Emulsion) Chemical herbicide, bifenox (C in the table; 2% by weight), lecithin: 5% by weight, 92% by weight of heavy white oil, and a strain of the genus Dolex Rera (MH-122756 strain). Conidial spores were suspended in an amount of 10 ¹⁰ per 1 ml of emulsion, an equal amount of 1% by weight of Triton X-100 was added and mixed to emulsify to obtain an emulsion.

In addition to the above formulation examples, formulations were made in the same manner for combinations of each conidia and each chemical herbicide.
The formulation is not limited to the above examples.

[Example 1] Herbicidal action of Nobie, a weed control agent in which the novel microbial strain of the present invention and a chemical herbicide are combined-granules-Novier seeds are sown in paddy soils packed in 1 / 1000a pots. , 1.5 seedlings were raised. Water depth is about 5
30 mg of each granule prepared by using the combination of the components shown in Tables 6-1 to 6-3 according to the method of Formulation Examples 24 to 31 described above in a pot in a flooded state of cm (1/10 of the standard application amount). (Corresponding) was treated and cultivated in a constant temperature room at 15 ° C. for both daytime and nighttime. After 20 days from application, the number of remaining individuals was counted, and the control rate against Nobie was shown in Tables 6-1 to 6-3 by the following formula.

[0119]

[Equation 1] In the table, A to O represent the following herbicides. The applied amount (g / 10a) is shown in parentheses.

A; CNP (27) B; Chloromethoxynil (21) C; Bifenox (21) D; Oxadiazone (6) E; Mefenacet (12) F; Naproanilide (30) G; NSK-850 (2) H Butachlor (15) I; pretilachlor (6) J; propanil (50) K; clomeprop (4.5) L; benchocarb (21) M; dimepiperate (21) N; molinate (24) O; esprocarb (21)

[0121]

[Table 7] *): When not using microorganisms **): When not using chemical herbicides

[0122]

[Table 8] *): When not using microorganisms **): When not using chemical herbicides

[0123]

[Table 9] *): When no microorganism is used **): When no chemical herbicide is used From Table 6-1, the novel microbial strain according to the present invention and a diphenyl ether-based chemical herbicide, CNP, clomethoxynil, bifenox, etc., or a diazine-based The herbicidal activity of the composition mixed with the herbicide, oxadiazone, etc. increased synergistically as compared with the case where each composition was applied alone.

From Table 6-2, the mixed composition of the novel microbial strain according to the present invention and the anilide chemical herbicide, mefenacet naproanilide, NSK-850, butachlor, protilachlor, propanil, clomeprop, etc. are each alone. The herbicidal activity was synergistically increased compared to the case of application.

From Table 6-3, the herbicidal activity of the novel microbial strain according to the present invention and the chemical composition of the thiol carbamate type chemical herbicide, benchocarb, dimepiperate, molinate, esprocarb, etc., is higher than that of the single application. Increased synergistically.

From the above results, a novel microbial fungus having a pathogenicity in Novier and a diphenyl ether chemical herbicide, anilide chemical herbicide, a thiolcarbamate chemical herbicide, a diazine-based herbicide which has been used as a herbicide for Novie in the past. A mixed composition with a chemical herbicide or the like shows a remarkable synergistic effect. As a result, one-tenth that was conventionally applied
Even with the use of a chemical herbicide of about 1/100, it has become possible to control Nobies sufficiently.

[Example 2] Herbicidal action of a novel microbial strain of the present invention and a chemical herbicide against Novier as a weed-controlling agent-flowable agent-Novier seeds were sown in paddy soils packed in pots of 1 / 1000a. , 1.5 seedlings were raised. Water depth is about 5
1 μl of a flowable agent prepared by using the combination of the components shown in Tables 7-1 to 7-3 according to the method of the above-mentioned formulation examples 38 to 41 in a water-filled pot of 1 cm (1/10 of the standard application amount).
(Corresponding to the above) was treated and cultivated in a constant temperature room at 15 ° C. for both daytime and nighttime. 20 days after application, the number of remaining individuals was counted, and the control rate against Nobie was calculated from the following formulas from Table 7-1 to Table 7-3.
It was shown to.

[0128]

[Equation 2] From Table 7-1, the herbicidal activity of the mixed composition of the novel microbial strain according to the present invention, the diphenyl ether chemical herbicide, and CNP was synergistically increased as compared with the case of single application.

As shown in Table 7-2, the composition of the novel microbial strain of the present invention and the chemical compound of anilide chemical herbicide, mefenacet, and protilachlor synergistically increased herbicidal activity as compared with the case where each composition was applied alone. Raised

From Table 7-3, the mixed composition of the novel microbial strain according to the present invention and the thiol carbamate herbicide, bench aucarb and molinate synergistically increased herbicidal activity as compared with the case where each composition was applied alone.

[0131]

[Table 10] *): When not using microorganisms **): When not using chemical herbicides

[0132]

[Table 11] *): When not using microorganisms **): When not using chemical herbicides

[0133]

[Table 12] *): When no microorganism is used **): When no chemical herbicide is used [Example 3] Herbicidal action of Nobie by a weed control agent in which the novel microbial strain of the present invention and a chemical herbicide are combined-Emulsion- Seeds were sown in paddy soil filled in a pot of 1 / 1000a, and seedlings were raised to the 1.5 leaf stage. Water depth is about 5
In a submerged pot of cm, 1.5 ml of an emulsion (corresponding to 1/10 of the standard application amount) prepared by using the combination of components shown in Table 8 according to the method of the above-mentioned formulation examples 45 to 46 was treated, It was cultivated in a constant temperature room at 15 ° C for both daytime and nighttime. 20 days after application, the number of remaining individuals was counted, and the control rate against Nobie was shown in Table 8 by the following formula.

[0134]

[Equation 3] In the table, A to O represent the following herbicides. The applied amount (g / 10a) is shown in parentheses. A: CNP (27) I; Protilachlor (6.0) E; Mefenacet (12) L; Benchocarb (21) From Table 8, a mixed composition of the novel microbial strain of the present invention, a diphenyl ether chemical herbicide, and CNP. The herbicidal activity of the product was synergistically increased as compared with the case of applying each alone. A novel microbial strain and anilide-based chemical herbicide according to the present invention,
The mixed composition of mefenacet and protilachlor synergistically increased herbicidal activity as compared with the case where each composition was applied alone. The mixed composition of the novel microbial strain according to the present invention and the chemical thiol carbamate chemical herbicide, bench ocarb, and molinate synergistically increased herbicidal activity as compared with the case where each composition was applied alone.

[0135]

[Table 13] *): When no microorganisms are used **): When no chemical herbicide is used [Example 4] Herbicidal action against weed control agents and broad-leaved weeds of weed control agents in which the novel microbial strain of the present invention and a chemical herbicide are combined. Seeds of Japanese eel and Japanese sardine, 1 / 1000a
The seeds were sown in the paddy soil filled in the pot, and seedlings were raised until the 1.5 leaf stage. 1.5 m of each mixture prepared by using the combination of the components shown in Tables 9-1 to 9-3 according to the method of the above-mentioned formulation examples 24 to 31 in a submerged pot having a water depth of about 3 cm.
g (corresponding to 1/10 of the standard application rate) was treated and cultivated in a constant temperature room at 15 ° C. for both daytime and nighttime. 20 days after application, the number of remaining individuals was counted, and the herbicidal effect against Nobie, Konagi and Japanese sardine was shown in Tables 9-1 to 9-3 as the control rate according to the following formula.

[0136]

[Equation 4] In the table, P to S represent the following herbicides. The applied amount (g / 10a) is shown in parentheses. P: Bensulfuron methyl (0.7) Q; Pyrazosulfuron ethyl (0.2) R; Pyrazolate (30) S; Cimethrin (7.5)

[0137]

[Table 14] *): When not using microorganisms **): When not using chemical herbicides

[0138]

[Table 15] *): When not using microorganisms **): When not using chemical herbicides

[0139]

[Table 16] *): When no microorganism is used **): When no chemical herbicide is used From Table 9-1, the novel microbial strain according to the present invention and a sulfonylurea chemical herbicide, bensulfuron-methyl, pyrazosulfuron-ethyl, etc., or , A diazine-based herbicide, pyrazolate, etc., or a mixed composition with a triazine-based herbicide, simethrin, etc. synergistically increased herbicidal activity against Nobies as compared with the case of applying each alone. Table 9-2
And from Table 9-3, the novel microbial strain according to the present invention, a sulfonylurea chemical herbicide, bensulfuron-methyl,
Pyrazosulfuron ethyl, etc., or diazine herbicides, pyrazolates, etc., or triazine herbicides, mixed compositions with simethrin, etc., compared to the case of applying each alone, against broad-leaved weeds such as eels, Heraomodaka However, some effect enhancement is observed. From Table 9-2 and Table 9-3, the novel microbial strain according to the present invention and a sulfonylurea chemical herbicide, bensulfuron-methyl, pyrazosulfuron-ethyl, etc., or a diazine herbicide, pyrazolate, etc., or a triazine herbicide The mixed composition with the agent, cimetrin, etc., showed a sufficient weed control effect against broad-leaved weeds such as eels and Japanese mosquitoes as compared with the case where each composition was applied alone, and its practicality was increased.

From the above results, the novel microbial strain of the present invention having pathogenicity to Nobie and the sulfonylurea chemical herbicides, bensulfuron-methyl, pyrazosulfuron-ethyl, etc., which have been conventionally used as herbicides for broadleaf weeds, or
Diazine herbicides, pyrazolates, etc., or triazine herbicides, a mixed composition with simethrin, etc., enhances the weed-controlling effect on Nobie and also broad-leaved weeds, and slightly However, it became practical as the effect increased. Further, by using it together with a broad-leaved weeding herbicide, it is possible to reduce the amount of conidia used by the novel microorganism of the present invention, as shown in Table 10, and it is possible to reduce the production cost. [Example 5] Reduction of the amount of applied conidia by the synergistic action of the novel microbial strain of the present invention and a chemical herbicide Novier seeds are sown in paddy soil packed in a pot of 1 / 1000a until the 1.5 leaf stage. Raised seedlings. Water depth is about 5
A predetermined amount of conidia and a broad-leaf herbicide bensulfuron (corresponding to 1/10 of the standard application amount) were treated in a flooded pot of cm, and cultivated in a constant temperature room at 15 ° C. during the day and night. 20 days after application, the number of remaining individuals was counted, and the control effect on Nobies is shown in Table 10 as the control rate by the following formula.

[0141]

[Equation 5]

[0142]

[Table 17]

[0143]

INDUSTRIAL APPLICABILITY The new variety according to the present invention is found to be pathogenic to all species of the genus Echinochloa, which is a weed nobie, even at 15 ° C. or below, and has a practical herbicidal action against nobie including the time of rice planting. It was Furthermore, it was confirmed to be extremely safe for cultivated plants such as rice.
The new variety according to the present invention is selected from microorganisms in the natural world, and is safe without fear of environmental pollution, which is a concern with organic synthetic pesticides.

Further, the mixed composition of the new varieties of the genus Dreyxella and the chemical herbicides according to the present invention shows a sufficient controlling effect at the herbicidal application rate which cannot be controlled by each of them alone. The application rate can be reduced. Furthermore, due to the synergistic effect of mixing the new varieties of the genus Dolexlera with the chemical herbicides, the amount of conidia of the bacterium of the genus Dolexlera to be applied can be reduced and the production cost can be reduced.

The new variety, weed-controlling agent and weed-controlling method according to the present invention not only contribute to crop production but also contribute to avoiding environmental pollution and the emergence of drug-resistant weeds, which have become a problem in recent years.

[Brief description of drawings]

1 shows a zymogram pattern of esterase of a new variant of the present invention (Drexlera monoceras var. Microsporus).

FIG. 2 shows a conventional Drex Rera monoceras (var.
FIG. 4 is a view showing a zymogram pattern of esterase of Monoceras).

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification code Internal reference number FI Technical display area A01N 43:78) (A01N 63/00 47:12) (C12N 1/14 C12R 1: 645) ( 72) Inventor Emi Niimi 1144 Togo, Mobara-shi, Chiba Mitsui Toatsu Kagaku Co., Ltd. (72) Inventor Kazunori Takanaka 1144 Togo Togo, Mobara-shi, Chiba Mitsui Toatsu Kagaku Co., Ltd. (72) Inventor Tomoko Ebista 1144 Togo, Mobara-shi, Chiba Mitsui Toatsu Chemical Co., Ltd.

Claims (27)

[Claims] 1. Drexlera monoceras va which is not pathogenic to crops but pathogenic to noviers
r. Microsporus. 2. The Drexlera monoceras var. According to claim 1, which has the esterase zymogram pattern shown in FIG. Microsporus. 3. FERM BP-3864, FERM
BP-4498, FERM BP-4499, FERM
BP-4500, FERM BP-4501, FER
M. BP-4502 or FERM BP-4503, wherein the Drexlera monoceras v according to claim 1.
ar. Microsporus. 4. Drexlera monoceras va which is not pathogenic to crops but pathogenic to noviers.
r. A weed control agent containing microsporus as an active ingredient. 5. Dolex Rera monoceras var.
The weed control agent according to claim 4, wherein the microsporus has the esterase zymogram pattern shown in FIG. 1. 6. Drexlera monoceras var.
As a micro porous, FERM BP-3864, F
ERM BP-4498, FERM BP-4499,
FERM BP-4500, FERM BP-450
1, FERM BP-4502 and FERM BP-45
The weed control agent according to claim 4, which comprises at least one of 03. 7. Drexlera monoceras va, which is not pathogenic for crops but pathogenic for noviers
r. A weed control agent containing microsporus and a chemical herbicide as active ingredients. 8. Drexlera monoceras var.
The weed control agent according to claim 7, wherein the microsporus has the esterase zymogram pattern shown in FIG. 1. 9. Drexlera monoceras var.
As a micro porous, FERM BP-3864, F
ERM BP-4498, FERM BP-4499,
FERM BP-4500, FERM BP-450
1, FERM BP-4502 and FERM BP-45
The weed control agent according to claim 7, which comprises at least one of 03. 10. The chemical herbicide is one or more selected from the group consisting of diphenyl ether herbicides, anilide herbicides, thiol carbamate herbicides, diazine herbicides, sulfonylurea herbicides and triazine herbicides. The weed control agent according to any one of claims 7 to 9. 11. A diphenyl ether herbicide is CN
P (chemical name; 2,4,6-trichlorophenyl-4-nitrophenyl ether), clomethoxynil (chemical name;
2,4-dichlorophenyl-4-nitro-3-methoxyphenyl ether) and bifenox (chemical name; 2,
4-dichlorophenyl-3-methoxycarbonyl-4-
The weed-controlling agent according to claim 10, wherein the weed-controlling agent is one or more selected from phenyl ether). 12. The herbicide anilide is mefenacet (chemical name: 2-benzothiazol-2-yloxy-N).
-Methylacetanilide), pretilachlor (chemical name; 2-chloro-2 ', 6'-diethyl-N- (n-propoxyethyl) acetanilide), naproanilide (chemical name; 2- (2-naphthoxy) propionic acid anilide), NSK-850 (Chemical name; N- [2,7- (3 '
-Methoxy) thienylmethyl-N-chloroaceto-2,
6-dimethylanilide), butachlor (chemical name; 2-
Chloro-2 ', 6'-diethyl-N- (butoxymethyl) acetanilide, propanil (chemical name; 3', 4 '
-Dichloropropionanilide) and clomeprop (chemical name; 2- (2,4-dichloro-3-methylphenoxy) propionanilide), and the weed control agent according to claim 10. 13. A thiolcarbamate herbicide is Bentocarb (chemical name: S- (4-chlorobenzyl)-
N, N-diethyl thiol carbamate), dimepiperate (chemical name; S- (1-methyl-1-phenethyl))-
Piperidine-1-carbathioate), molinate (chemical name; S-ethyl-N, N-hexamethylenethiocarbamate) and esprocarb (chemical name; S-benzyl-N-ethyl-N- (1,2-dimethylpropyl)) The weed control agent according to claim 10, which is one or more selected from thiol carbamate). 14. A diazine herbicide is oxadiazone (chemical name: 5-tert.-butyl-3- (2,4-
Dichloro-5-isopropoxyphenyl) -1,3,4
-Oxazol-2- (3H) -one) or pyrazolate (chemical name; 4- (2,4-dichlorobenzoyl)-
1,3-Dimethyl-1H-pyrazol-5-yl-p-
11. The weed control agent according to claim 10, which is toluene sulfonate) or both of them. 15. A sulfonylurea herbicide is bensulfuron-methyl (chemical name: methyl 2- [3- (4,6-
Dimethylpyrimidin-2-yl) ureidosulfonylmethyl] benzoate) or pyrazosulfuron-ethyl (chemical name; ethyl 5- [3- (4,6-dimethoxypyrimidin-2-yl) ureidosulfonyl] -1-methylpyrazole-4) -Carboxylate), or both, The weed control agent according to claim 10. 16. The weed control agent according to claim 10, wherein the triazine herbicide is cimetrin (chemical name: 2,4-bis (ethylamino) -6-methylthio-1,3,5-triazine). 17. The Drexlera monoceras var. A method for controlling weeds, which comprises administering Microsporus to a place where it is required for controlling Nobie. 18. Drexlera monoceras v which is not pathogenic to crops but pathogenic to Novier
ar. A method for controlling weeds, which comprises administering Microsporus and one or more chemical herbicides to a place where it is necessary for controlling Nobie. 19. The Dolex Rera monoceras va
r. The method of controlling weeds according to claim 18, wherein the microsporus has the esterase zymogram pattern shown in FIG. 20. Dolex Rera monoceras va
r. As a microsporus, Drekkurera Monoceras var. Microsporus FERM BP-3864,
FERM BP-4498, FERM BP-449
9, FERM BP-4500, FERM BP-45
01, FERM BP-4502 and FERM BP-
The method of controlling weeds according to claim 18, wherein at least one of 4503 is used. 21. One or more chemical herbicides selected from the group consisting of diphenyl ether herbicides, anilide herbicides, thiol carbamate herbicides, diazine herbicides, sulfonylurea herbicides and triazine herbicides. The weed control method according to any one of claims 18 to 20. 22. The diphenyl ether herbicide is CN
P (chemical name; 2,4,6-trichlorophenyl-4-nitrophenyl ether), clomethoxynil (chemical name;
2,4-dichlorophenyl-4-nitro-3-methoxyphenyl ether) and bifenox (chemical name; 2,
4-dichlorophenyl-3-methoxycarbonyl-4-
22. The method of controlling weeds according to claim 21, wherein the weed control is one or more selected from phenyl ether). 23. The anilide herbicide is mefenacet (chemical name: 2-benzothiazol-2-yloxy-N).
-Methylacetanilide), pretilachlor (chemical name; 2-chloro-2 ', 6'-diethyl-N- (n-propoxyethyl) acetanilide), naproanilide (chemical name; 2- (2-naphthoxy) propionic acid anilide), NSK-850 (Chemical name; N- [2,7- (3 '
-Methoxy) thienylmethyl-N-chloroaceto-2,
6-dimethylanilide), butachlor (chemical name; 2-
Chloro-2 ', 6'-diethyl-N- (butoxymethyl) acetanilide, propanil (chemical name; 3', 4 '
-Dichloropropionanilide) and clomeprop (chemical name; 2- (2,4-dichloro-3-methylphenoxy) propionanilide), the method of controlling weeds according to claim 21. 24. A thiolcarbamate herbicide is Bentocarb (chemical name: S- (4-chlorobenzyl)-
N, N-diethyl thiol carbamate), dimepiperate (chemical name; S- (1-methyl-1-phenethyl))-
Piperidine-1-carbathioate), molinate (chemical name; S-ethyl-N, N-hexamethylenethiocarbamate) and esprocarb (chemical name; S-benzyl-N-ethyl-N- (1,2-dimethylpropyl)) The method for controlling weeds according to claim 21, wherein the weed control method is one or more selected from thiol carbamate). 25. A diazine-based herbicide is oxadiazone (chemical name: 5-tert.-butyl-3- (2,4-
Dichloro-5-isopropoxyphenyl) -1,3,4
-Oxazol-2- (3H) -one) or pyrazolate (chemical name; 4- (2,4-dichlorobenzoyl)-
1,3-Dimethyl-1H-pyrazol-5-yl-p-
22. The method for controlling weeds according to claim 21, which is toluene sulfonate) or both of them. 26. A sulfonylurea herbicide is bensulfuron-methyl (chemical name: methyl 2- [3- (4,6-
Dimethylpyrimidin-2-yl) ureidosulfonylmethyl] benzoate) or pyrazosulfuron-ethyl (chemical name; ethyl 5- [3- (4,6-dimethoxypyrimidin-2-yl) ureidosulfonyl] -1-methylpyrazole-4) -Carboxylate) or both thereof. 27. The weed control method according to claim 21, wherein the triazine herbicide is cimetrin (chemical name: 2,4-bis (ethylamino) -6-methylthio-1,3,5-triazine).