CN114872157B - Method for treating trunk of new dead pine wood nematode epidemic disease wood - Google Patents

Method for treating trunk of new dead pine wood nematode epidemic disease wood Download PDF

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CN114872157B
CN114872157B CN202210638988.9A CN202210638988A CN114872157B CN 114872157 B CN114872157 B CN 114872157B CN 202210638988 A CN202210638988 A CN 202210638988A CN 114872157 B CN114872157 B CN 114872157B
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wood
trunk
treating
new dead
epidemic
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CN114872157A (en
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蔡守平
何学友
曾丽琼
汤陈生
宋海天
詹芳芳
徐云
毛志群
梁智生
杨惠珍
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FUJIAN ACADEMY OF FORESTRY
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B27WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27KPROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
    • B27K5/00Treating of wood not provided for in groups B27K1/00, B27K3/00
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B27WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
    • B27KPROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
    • B27K2240/00Purpose of the treatment
    • B27K2240/20Removing fungi, molds or insects
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/28Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a method for treating a trunk of a new dead pine wood nematode epidemic, belonging to the technical field of pest control. The invention relates to a method for treating a trunk of a new dead pine wood nematode epidemic wood, which sequentially comprises the steps of peeling a wood segment to a wood layer, spraying a metarhizium anisopliae spore suspension liquid and stacking the wood segments into a wood pile, covering geotextiles, wetting the geotextiles with clear water, and finally covering a layer of degradable mulching film on the geotextiles. The method for treating the trunk of the new dead pine wood nematode disease epidemic wood realizes the in-situ pollution-free treatment of the trunk of the new dead pine wood nematode disease epidemic wood, avoids the step of transferring epidemic wood to the under-mountain treatment, and improves the epidemic wood treatment efficiency.

Description

Method for treating trunk of new dead pine wood nematode epidemic disease wood
Technical Field
The invention belongs to the technical field of pest control, and particularly relates to a method for treating a trunk of a new dead pine wood nematode epidemic wood.
Background
Pine wood nematode disease is a very dangerous forest disease, is a major plant epidemic situation, and has very strong infectivity, diffusivity and destructiveness. Since the first discovery in Jiangsu Nanjing in 1982, the disease spreads rapidly in China, and the accumulated lethal pine is over 6 hundred million, and in recent years, the annual average lethal pine is over 2000 ten thousand, resulting in huge economic and ecological losses.
Aiming at the prevention and treatment of pine wood nematode diseases, the method mainly needs to thoroughly clean dead/ill-dead pine trees and thoroughly remove harmful treatments to cut off disease transmission. Timely cleaning and thorough pest-killing treatment of dead/dead pine trees are important for preventing the spread of the disease, and the previous pest-killing treatment of dead/dead pine trees mainly comprises bagging fumigation (using aluminum phosphide fumigation), incineration, slicing/crushing, iron wire mesh cover and the like. For example, the chinese patent application publication No. CN 113455261A discloses a method of treating dead wood by bagging fumigation, but the method is easy to break bags, pollute medicines, etc., and the national forestry and grassland bureau has forbidden to use the method for pest control treatment. At present, the production mainly adopts the modes of incineration, slicing/crushing, iron wire mesh covers and the like, the incineration and slicing generally need to transport epidemic wood to a relatively open place for centralized incineration or centralized point for slicing/crushing treatment, so that the epidemic wood must go down the mountain, branches can be bundled for centralized mountain-down, the operation difficulty is not large, and the trunk part with larger specific gravity of the whole tree is difficult to go down the mountain and consumes man hours, the situation that the epidemic wood remains in the mountain is easy to occur, the treatment cost is high, and the possibility of poor treatment effect caused by the phenomenon that the pest control treatment is not in place is easy to occur; the iron wire mesh enclosure has higher cost and is difficult to implement in a large area. Therefore, for some areas with complex terrains, steep mountain roads, inconvenient traffic and the like where epidemic woods are difficult to descend, it is necessary to explore a set of technical modes capable of realizing on-site efficient treatment of pine wood nematode epidemic woods.
The propagation of pine wood nematode disease needs to depend on Monochamus alternatus as a propagation medium, and the metarhizium anisopliae is an important entomogenous fungus, is widely applied to biological control of agricultural and forestry pests, has the characteristics of strong pathogenicity, long-term surviving in the environment and epidemic disease formation, and is a good biological control resource. Researches prove that the metarhizium anisopliae has stronger pathogenicity on the Monochamus alternatus larvae, however, due to the hiding property of the movement of the Monochamus alternatus larvae, the fungus agent is difficult to reach the movement part of the Monochamus alternatus larvae because of the influence of trunk cortex and moth, so that the technical problem exists in the process of preventing and controlling the Monochamus alternatus larvae by using the metarhizium anisopliae.
Disclosure of Invention
In order to overcome the defects in the prior art, the technical problems to be solved by the invention are as follows: how to provide an on-site treatment and pollution-free treatment method for the trunk of new dead pine wood nematode disease wood.
In order to solve the technical problems, the invention adopts the following technical scheme: the method for treating the trunk of the new dead pine wood nematode epidemic is characterized by comprising the following steps of:
s1: the new dead pine is felled down, the branch tips and the branch parts are removed, and the trunk is left;
s2: cutting the trunk into wood sections and peeling the wood sections to a wood layer;
s3: spraying the metarhizium anisopliae spore suspension on the wood segments after peeling;
s4: piling up the wood segments sprayed with the metarhizium anisopliae spore suspension into a wood pile, covering the wood pile with geotextile, and wetting the geotextile with clear water;
s4: and covering a layer of degradable mulching film on the geotextile.
Wherein the destruxin spore suspension is prepared by 10 from destruxin spore powder with the viable spore rate of more than 95 percent 7 ~10 8 Metarhizium anisopliae spore suspension per mL.
Wherein, the tree knots and the branches of the wood sections in S1 are also peeled to the wood layer.
Wherein, the trunk in S1 is cut into 1.5-2 m wood sections.
When the metarhizium anisopliae spore suspension is sprayed in the step S3, water is slightly dripped on the surface of the wood segment.
Wherein the number of the wood sections in the wood pile is 8-15 sections.
Wherein the branch tip and the branch portion are subjected to a pest-removing treatment by incineration or pulverization.
Wherein, when the new dead pine is felled in S1, the height of the stump is not more than 5cm.
The invention has the beneficial effects that: according to the invention, through a series of operations of peeling the trunk, spraying the metarhizium anisopliae and the geotextile to cover and wet and then covering the degradable mulching film, the on-site pollution-free treatment of the new dead pine wood nematode epidemic trunks is realized, the step of transferring the epidemic wood to the under-mountain treatment is avoided, and the epidemic wood treatment efficiency is improved. Spraying the destruxin spore suspension after peeling the trunk, and improving the lethality of the destruxin on the monochamus alternatus hope larvae in the dead trunk.
Detailed Description
In order to describe the technical contents, the achieved objects and effects of the present invention in detail, the following description will be made with reference to the embodiments.
The most critical concept of the invention is as follows: the on-site pollution-free treatment of the new dead pine wood nematode epidemic wood trunk is realized through a series of operations of peeling the trunk, spraying the metarhizium anisopliae and the geotextile to cover and wet and then covering the degradable mulching film, the step of transferring the epidemic wood to the under-mountain treatment is avoided, and the epidemic wood treatment efficiency is improved.
The invention provides a method for treating a trunk of a new dead pine wood nematode epidemic, which is characterized by comprising the following steps:
s1: the new dead pine is felled down, the branch tips and the branch parts are removed, and the trunk is left;
s2: cutting the trunk into wood sections and peeling the wood sections to a wood layer;
s3: spraying the metarhizium anisopliae spore suspension on the wood segments after peeling;
s4: piling up the wood segments sprayed with the metarhizium anisopliae spore suspension into a wood pile, covering the wood pile with geotextile, and wetting the geotextile with clear water;
s4: and covering a layer of degradable mulching film on the geotextile.
From the above description, the beneficial effects of the invention are as follows:
1. the on-site pollution-free treatment of the new dead pine wood nematode epidemic wood trunk is realized through a series of operations of peeling the trunk, spraying the metarhizium anisopliae and the geotextile to cover and wet and then covering the degradable mulching film, the step of transferring the epidemic wood to the under-mountain treatment is avoided, and the epidemic wood treatment efficiency is improved.
2. The peeling treatment can effectively reduce the Monochamus alternatus on the new dead trunk and prevent the Monochamus alternatus from spawning on the new dead trunk.
The Monochamus alternatus in egg stage is 6-10 d, and the larvae grow for 35-50 d in the feeding of cortex and rim material, namely, at least more than 40 days from the time of oviposition of adults until the larvae enter xylem. Therefore, the new dead pine trunks are less in Monochamus alternatus larvae or are mostly at the cortex or trunk xylem side materials, even if the trunk is invaded, the trunk is shallow, and the Monochamus alternatus larvae can be removed through peeling treatment, so that the purpose of killing the Monochamus alternatus larvae is achieved.
The monochamus alternatus hope lays eggs on dying pine trees, namely, the monochamus alternatus hope firstly bites grooves on barks by a mouth gag and then lays eggs under the barks in the grooves, so that the eggs are less influenced by bad environments such as heavy rain, high temperature and the like, and meanwhile, the natural enemies can be less parasitized and predated due to the protection of the barks; meanwhile, the larvae of longicorn such as Monochamus alternatus are undeveloped in chest and abdomen, if adults directly lay eggs on the surface of a host, the hatched larvae are not supported by the grooves of the bark, and are difficult to attach to the surface of the host to eat and be eaten into xylem, so that the female adults of Monochamus alternatus are hardly lay eggs on the skinless wood.
3. The method has the advantages that the method combines the peeling treatment, the spraying of the metarhizium anisopliae spore suspension and the sequential covering of the wet geotextile and the degradable mulching film, so that the lethality of metarhizium anisopliae to the monochamus alternatus larvae in the dead trunk is improved.
1. For a few of longicorn larvae which invade the shallow xylem, the longicorn invasion holes can be exposed by peeling treatment, so that other natural enemies and microorganisms are increased to invade the longicorn, and the survival rate of the longicorn is reduced. Meanwhile, the destruxin bacteria liquid sprayed with the destruxin bacteria liquid can permeate into the longicorn along with the invasion holes of the longicorn so as to contact with the longicorn larva, thereby achieving the aim of killing the longicorn larva. The problem that the metarhizium anisopliae is difficult to contact with insect bodies and cannot be infected in the traditional prevention and treatment is also solved.
2. The trunk woodpile is sequentially covered with the wetted geotechnical cloth and the degradable agricultural film, so that the longicorn adults can be prevented from moving to trunk parts again, the humidity of the trunk woodpile environment can be increased, and the probability of the longicorn larvae being infected by metarhizium anisopliae or other microorganisms is increased. Meanwhile, the higher humidity can increase the growth of decay fungi on the trunk, accelerate the decay of the trunk and reduce the quantity of pine wood nematodes in the trunk.
The degradable agricultural film can be naturally degraded in 3-6 months, is environment-friendly, and can realize nuisance free in the whole pest-removing treatment process.
Further, the metarhizium anisopliae spore suspension is prepared into 10 from metarhizium anisopliae spore powder with the viable spore rate of more than 95 percent 7 ~10 8 Metarhizium anisopliae spore suspension per mL.
From the above description, it is clear that: the better the high-spore-rate metarhizium anisopliae spore suspension has the lethal effect on the monochamus alternatus larvae.
Further, the knots and the branches of the wood segments in S1 are also peeled to the wood layer.
From the above description, it is clear that: the peeling treatment needs to be thorough, dead angles are not reserved, the Monochamus alternatus is prevented from spawning at the dead angles, and the destruxins of Metarrhizium anisopliae spore suspension is prevented from being sprayed at the dead angles.
Further, the trunk in S1 is cut into 1.5-2 m wood sections.
From the above description, it is clear that: the trees are cut into 1.5-2 m, so that the trees are convenient to transport and stack.
Further, when the metarhizium anisopliae spore suspension is sprayed in the step S3, water is slightly dropped on the surface of the wood segment.
From the above description, it is clear that: ensuring that the surfaces of the wood segments are fully contacted with the metarhizium anisopliae spore suspension liquid, and immersing part of the metarhizium anisopliae spore suspension liquid from the spawning notch of the monochamus alternatus.
Further, the number of the wood sections in the wood pile is 8-15 sections.
From the above description, it is clear that: the space can be saved by the wood Duan Duidie, the efficiency is improved, but the stacking quantity of the wood segments is too large, and the regulation and control of humidity in geotechnical cloth are not facilitated.
Further, the branch tip and the branch portion are subjected to a pest-removing treatment by incineration or pulverization.
From the above description, it is clear that: the branch tip and the branch are lighter and can be treated by a pest-killing treatment mode.
Further, when the new dead pine tree is felled down in S1, the height of the stump is not more than 5cm.
From the above description, it is clear that: the stump height is low, and the spawning quantity of Monochamus alternatus on the stump is reduced.
The first embodiment of the invention is as follows: a method for treating the trunk of new dead pine wood nematode epidemic disease wood comprises the following steps:
s1: the new dead pine is felled down, stumps with the height not exceeding 5cm are left, branch tips and branch parts are removed, trunks are left, and the branch tips and the branch parts are subjected to pest removal treatment in a burning or crushing mode;
s2: the trunk is cut into wood sections with the length of 2m, the bark peeling treatment is carried out by using a bark peeler, a bark cutter of the bark peeler is required to touch the xylem, the bark peeling is thorough, dead angles, particularly the bifurcation parts, are not remained, and no longicorn invaded holes are found on the trunk after the bark peeling.
S3: preparing the destruxin spore powder into 10 7 The metarhizium anisopliae spore bacterial suspension of each spore/mL is sprayed on the peeled wood segments, and water is slightly dripped on the surfaces of the wood segments.
S4, after spraying bacteria on the surfaces of the wood segments, stacking the wood segments into wood piles by selecting relatively flat ground, wherein 9 segments of wood segments are arranged in each wood pile, then covering the wood piles by geotextiles, spraying clear water to wet the geotextiles, and then covering a layer of degradable agricultural film on the geotextiles to keep the humidity in the wood Duan Dui.
The first comparative example of the present invention is: the epidemic wood section is not peeled, and comprises the following steps:
s1: the new dead pine is felled down, a stump with the height of 5cm is left, branch tips and branch parts are removed, a trunk is left, and the branch tips and the branch parts are subjected to pest removal treatment in a burning or crushing mode;
s2: the trunk is cut into wood sections with the length of 2m, and the wood sections are free from the oviposition and harm traces of longicorn during treatment.
The effect of the treatment methods of example one and comparative example one on the second year is shown in table 1.
TABLE 1
Figure BDA0003681671520000051
Figure BDA0003681671520000061
In Table 1, the wood segments without the invasion of the longicorn are treated by nuisance free pest control, and the places for grooving and spawning are lost by peeling, and the longicorn covered by geotextile prevents the longicorn from moving on the wood segments, so that the longicorn does not invade to be harmful. In the wood sections without peeling, more than 1/3 of the wood sections can be found to have the pine eclosion holes, and the number of the pine eclosion holes per section is 2.4-3.3.
The second embodiment of the invention is as follows: a method for treating the trunk of new dead pine wood nematode epidemic disease wood comprises the following steps:
s1: the new dead pine is felled down, a stump with the height of 5cm is left, branch tips and branch parts are removed, a trunk is left, and the branch tips and the branch parts are subjected to pest removal treatment in a burning or crushing mode;
s2: cutting the trunk into 1.5m long wood sections, peeling by using a peeling machine, wherein the peeling machine is required to touch the xylem, the peeling is thorough, dead angles, particularly the bifurcation parts, are not remained, and after peeling, partial longhorn beetles invade holes on the epidemic wood trunk are found.
S3: preparing the destruxin spore powder into 10 8 The metarhizium anisopliae spore bacterial suspension of each spore/mL is sprayed on the peeled wood segments, and water is slightly dripped on the surfaces of the wood segments.
S4, after spraying bacteria on the surfaces of the wood segments, stacking the wood segments into wood piles by selecting relatively flat ground, wherein 9 segments of wood segments are arranged in each wood pile, then covering the wood piles by geotextiles, spraying clear water to wet the geotextiles, and then covering a layer of degradable agricultural film on the geotextiles to keep the humidity in the wood Duan Dui.
The second comparative example of the present invention is: the epidemic wood section is not peeled, and comprises the following steps:
s1: the new dead pine is felled down, a stump with the height of 5cm is left, branch tips and branch parts are removed, a trunk is left, and the branch tips and the branch parts are subjected to pest removal treatment in a burning or crushing mode;
s2: cutting the trunk into 1.5m long wood sections, wherein the epidemic wood trunk is provided with partial longicorn intrusion holes.
The third comparative example of the present invention is: the epidemic wood section is only peeled, and comprises the following steps:
s1: the new dead pine is felled down, stumps with the height not exceeding 5cm are left, branch tips and branch parts are removed, trunks are left, and the branch tips and the branch parts are subjected to pest removal treatment in a burning or crushing mode;
s2: cutting the trunk into 1.5m long wood sections, peeling by using a peeling machine, wherein the peeling machine is required to touch the xylem, the peeling is thorough, dead angles, particularly the bifurcation parts, are not remained, and after peeling, partial longhorn beetles invade holes on the epidemic wood trunk are found.
The comparative example IV of the present invention is: a method for treating the trunk of new dead pine wood nematode epidemic disease wood comprises the following steps:
s1: the new dead pine is felled down, stumps with the height not exceeding 5cm are left, branch tips and branch parts are removed, trunks are left, and the branch tips and the branch parts are subjected to pest removal treatment in a burning or crushing mode;
s2: cutting the trunk into 1.5m long wood sections, peeling by using a peeling machine, wherein the peeling machine is required to touch the xylem, the peeling is thorough, dead angles, particularly the bifurcation parts, are not remained, and after peeling, partial longhorn beetles invade holes on the epidemic wood trunk are found.
S3: preparing the destruxin spore powder into 10 8 The metarhizium anisopliae spore bacterial suspension of each spore/mL is sprayed on the peeled wood segments, and water is slightly dripped on the surfaces of the wood segments.
And S4, after spraying bacteria on the surfaces of the wood segments, stacking the wood segments into wood piles by selecting relatively flat ground, wherein 9 wood segments are arranged in each wood pile.
The effect of the treatment methods of example two and comparative examples two to fourth was shown in Table 2 for the second year.
TABLE 2
Figure BDA0003681671520000071
(the partial values are mean.+ -. Standard deviation, the difference is obvious for the same column of data with lower case English letters)
From Table 2, over 80% of the wood sections of the unpeeled longicorn pine tree had longicorn feathering holes in the second year. And the single peeling treatment can obviously reduce the eclosion number of the longhorned beetles. The emergence of longhorned beetles can also be significantly reduced if the peeling treatment is used together with the spraying of the metarhizium anisopliae suspension. In the two test points, after the pest removal treatment is carried out by the modes of peeling, spraying the metarhizium anisopliae suspension liquid and coating, no longicorn eclosion is found in all the treated wood sections in the next year.
In summary, through the mode of peeling, spraying the metarhizium anisopliae spore suspension, covering geotextile and degradable agricultural films, the aim that the monochamus alternatus cannot eclosion can be basically achieved, so that the aim of pest control treatment of the wood-epidemic trunk is fulfilled, and the following reasons are possible:
firstly, when the peeling treatment is carried out by using a peeling machine, the longicorn larva which is not invaded in the phloem can be directly killed or removed, and the longicorn larva cannot further invade the xylem;
secondly, after the pine tree sections are peeled, the longicorn invaded into the holes to lose the protection of the cortex, and some external microorganisms or natural enemies are more likely to invade into the holes, so that the survival rate of the longicorn is reduced;
thirdly, as the invasive holes are exposed, the destruxin spore suspension can be more easily infiltrated into the channels of the longhorn beetle larvae through the exposed invasive holes by spraying the destruxin spore suspension, so that the aim of infection is fulfilled;
and fourthly, 2 purposes can be achieved through the coverage of the wet geotextile and the degradable agricultural film, the humidity of the small environment of the epidemic wood pile is kept to effectively promote the metarhizium anisopliae to exert efficacy, and meanwhile, even if the longicorn emerges due to the high density of the geotextile, the longicorn can be effectively prevented from flying to forests. The degradable film can be degraded in the forest for about 6 months, so the whole pest-killing treatment realizes nuisance free.
The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent modifications made by the teachings of the present invention, or direct or indirect application in the relevant art, are intended to be included within the scope of the present invention.

Claims (6)

1. The method for treating the trunk of the new dead pine wood nematode epidemic is characterized by comprising the following steps of:
s1: the new dead pine is felled down, the branch tips and the branch parts are removed, and the trunk is left;
s2: cutting the trunk into wood sections and peeling the wood sections to a wood layer;
s3: spraying the metarhizium anisopliae spore suspension on the wood segment after peeling, and spraying the metarhizium anisopliae spore suspension on the surface of the wood segment with slightly water drops;
s4: piling up the wood segments sprayed with the metarhizium anisopliae spore suspension into a wood pile, covering the wood pile with geotextile, and wetting the geotextile with clear water;
s5: covering a layer of degradable mulching film on the geotextile;
the destruxin spore suspension is prepared by 10 percent of destruxin spore powder with the viable spore rate of more than 95 percent 7 ~10 8 Metarhizium anisopliae spore suspension per mL.
2. The method for treating a trunk of a new dead pine wood nematode disease wood according to claim 1, wherein the knots and the branches of the wood segments in S1 are also peeled to the woody layer.
3. The method for treating a trunk of a new dead pine wood nematode disease wood according to claim 1, wherein the trunk in S1 is cut into 1.5-2 m wood segments.
4. The method for treating a trunk of a new dead pine wood nematode disease wood according to claim 1, wherein the number of the wood segments in the wood pile is 8-15.
5. The method for treating a trunk of a new-deadwood nematode disease wood according to claim 1, wherein the branch tip and the branch portion are subjected to a pest-removing treatment by incineration or pulverization.
6. The method for treating a trunk of a new dead pine wood nematode disease wood according to claim 1, wherein the height of the stump is not more than 5cm after the new dead pine is felled in S1.
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