CN104585195A - Tetramycin compound and application thereof - Google Patents
Tetramycin compound and application thereof Download PDFInfo
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Abstract
The invention relates to the field of research and development of biological pesticide preparations, and particularly relates to a tetramycin compound and application thereof, which are a divisional application of an invention named 'tetramycin compound and application' having an application number of 2013106143470 and being applied on November 26, 2013. The tetramycin compound comprises tetramycin and an active substance, wherein the active substance belongs to amide, including one or more of benzamides, dicarboximides, formamides, kresoxim-methyl, N-halogenated methylthio-dicarboximides and dimethomorph. Compared with the prior art, the tetramycin compound and application thereof have the advantages that the risk of resistant fungal strain selectivity can be reduced, the service life of the active substance can be prolonged, the prevention and treatment range can be enlarged, the disease prevention effect can be improved, the drug resistance of pathogenic bacteria can be retarded or overcome, the cost can be reduced, and the number of pesticide application times can be reduced.
Description
The divisional application that the present patent application is application number is 2013106143470, the applying date is on November 26th, 2013, denomination of invention is " tetramycin complex and application ".
Technical field
The present invention relates to biopesticide preparaton research and development field, in particular to tetramycin complex and application.
Background technology
At present, the pesticide species for plant protection is various, and mostly pesticide species is meddlesome, but also brings a lot of problem.Mostly agricultural chemicals is that a worm one is controlled, a medicine one is controlled, thus agricultural chemicals can only be prevented and treated for a kind of insect pest or disease, does not produce effect, even cause the accident of the underproduction to other murder by poisoning or disease, and replay in field, No kernels or seeds are gathered, as in a year of scarcity for even ruining of having.The plant protection technology that a this worm one is controlled, a medicine one is controlled is not suitable with the needs of agricultural development, and peasant controls in the urgent need to a medicine more, sick worm is doublely controlled, economize medicine saving of labor, protect the dispenser new technology of producing volume increase, easy to use, remarkable benefit.
Summary of the invention
The object of the present invention is to provide tetramycin complex and application, to solve the above problems.
Embodiments providing a kind of tetramycin complex, comprising: tetramycin and active substance; Described active substance is amide-type, described amide-type comprise following one or more: benzamides, dicarboximide class, benzamide type, strobilurins, N-halogenmethylthio-dicarboximide class, dimethomorph.
The embodiment of the present invention additionally provides a kind of application of tetramycin complex, for controlling plant diseases, insect pest and weeds, and/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
The tetramycin complex that the embodiment of the present invention provides and application are compared with prior art, tetramycin possesses the effect of sterilization, control germ, disease, tetramycin and active substance composite after, by by having the active substance of the different mechanism of action and its combination, reduce refractory fungal bacterial strain optionally dangerous, service life of extending active component, add the fungicidal spectrum of single component, expand prevention and treatment range; Improve protection effect; Can delay or overcome the pesticide resistance of germ; Both saved the application times that cost again reduces agricultural chemicals, active response country advocates environmental protection agricultural chemicals.
Embodiment
Be described in further detail the present invention below by specific embodiment, content mentioned in embodiment is not limitation of the invention, and material prescription is selected to suit measures to local conditions and on result without substantial impact.In these embodiments, unless otherwise indicated, all percentage is all weight percentage.
Tetramycin complex comprises: tetramycin and active substance; Described active substance is amide-type, described amide-type comprise following one or more: benzamides, dicarboximide class, benzamide type, strobilurins, N-halogenmethylthio-dicarboximide class, dimethomorph.
The embodiment of the present invention additionally provides a kind of application of tetramycin complex, for controlling plant diseases, insect pest and weeds, and/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
Biopesticide tetramycin is a kind of high-effective broad-spectrum fungicide; to the plurality of plant diseases on the crops such as cereal, fruit tree, vegetables, all there is protection and therapeutic activity, especially to fungal diseases such as gray mold crops such as () tomato, cucumber, capsicums, powdery mildew (wheat class, melon, fruit tree etc.), scabs (apple, pears etc.), there is good control efficiency.But this series bactericidal agent action site is single, and germ easily produces adaptive variation to medicament, the preventive effect of medicament is reduced.Tetramycin and active substance composite after can play synergistic function, by by having the active substance of the different mechanism of action and its combination, reduce refractory fungal bacterial strain optionally dangerous, service life of extending active component, add the fungicidal spectrum of single component, expand prevention and treatment range; Improve protection effect; Can delay or overcome the pesticide resistance of germ; Both saved the application times that cost again reduces agricultural chemicals, active response country advocates environmental protection agricultural chemicals.
Next, the present invention will describe this tetramycin complex in detail by some specific embodiments, and the embody rule situation of tetramycin complex:
Tetramycin complex, comprising: tetramycin and active substance;
Described active substance is amide-type, described amide-type comprise following one or more: benzamides, dicarboximide class, benzamide type, strobilurins, N-halogenmethylthio-dicarboximide class.
In certain embodiments, when active substance comprises benzamides, benzamides comprise following one or more: M 9834, metalaxyl, R-metalaxyl, Wakil, chlorine worm benzamide;
In certain embodiments, when active substance comprises dicarboximide class, dicarboximide class comprise following one or more: chlozolinate, sclex, isopropyl are fixed, myclozolin, procymidone, vinclozolin;
In certain embodiments, when active substance comprises benzamide type, benzamide type comprise following one or more: carboxin, first furan anilide, flutolanil, mebenil, oxycarboxin or thifluzamide, alachlor, Acetochlor, the third careless amine;
In certain embodiments, when active substance comprises strobilurins, strobilurins comprise following one or more: nitrile Fluoxastrobin, imines bacterium, fork amine benzamide, pyraclostrobin, ZEN 90160,2 [(2-trifluoromethyl)-pyridine-6-base oxygen ylmethyl]-3-methoxy group-methyl acrylate, Lonster, Flumiclorac pentyl, oxime bacterium ester;
In certain embodiments, when active substance comprises N-halogenmethylthio-dicarboximide class, N-halogenmethylthio-dicarboximide class comprise following one or more: difoltan, captan, Euparen, fluoromide, folpet or Tolylfluanid;
In certain embodiments, active substance is dimethomorph.
Wherein the mass concentration of tetramycin in tetramycin complex can adjust arbitrarily in 0.15-99%, according to the type of compound and required effect, reaches better balanced toxicity and residual by adjustment mass concentration (or the concentration that weighs).
The rate of application of inventive mixture is 0.6-400 gram/ha.
Such as: in certain embodiments, the mass concentration of tetramycin is 0.15%, and concentration is lower.
In certain embodiments, the mass concentration of tetramycin is 15%, or 35%, or 50%, or 70%.
In certain embodiments, the mass concentration of tetramycin is 99%, and concentration is higher, in tetramycin complex, play Main Function.
The finished product of this tetramycin complex can make in various forms one or more, such as: aqua, dispersible agent, missible oil, microemulsion, emulsion, suspension, gel formulation, ultra low volume solvent (ULV).Be made into various forms and facilitate the storage of tetramycin complex, transport and follow-up use.
Such as:
A) aqua
The composite mixture of 10 weight portion is dissolved in 90 weight parts waters or water-soluble solvent.Or add wetting agent or other auxiliary agent, composite mixture dissolves through water dilution, obtains the aqua that tetramycin complex mass percentage content is 10% in this way.
B) dispersible agent
Be dissolved in 70 weight portion cyclohexanone by the composite mixture of 20 weight portion, and add 10 parts per weight dispersing agent as PVP(polyvinyl pyrrolidone), dilute with water obtains dispersion, obtains the dispersible agent that tetramycin complex mass percentage content is 20% in this way.
C) missible oil
The composite mixture of 15 weight portion to be dissolved in 75 parts by weight of xylene and to add calcium dodecyl benzene sulfonate and castor oil ethoxylate (being 5 weight portions in each case), dilute with water obtains emulsion, obtains the missible oil that tetramycin complex mass percentage content is 15% in this way.
D) microemulsion
The composite mixture of 10 weight portion to be dissolved in 20 weight portion cyclohexanones and to add calcium dodecyl benzene sulfonate (being 20 weight portions in each case), dilute with water obtains microemulsion, obtains the microemulsion that tetramycin complex mass percentage content is 5% in this way.
E) emulsion
The composite mixture of 25 weight portion to be dissolved in 35 parts by weight of xylene and to add calcium dodecyl benzene sulfonate and castor oil ethoxylate (being 5 weight portions in each case).This mixture to be introduced in 30 weight parts waters by emulsifier (Ultraturrax) and make equal phase emulsion.Dilute with water obtains emulsion, obtains the emulsion that tetramycin complex mass percentage content is 25% in this way.
F) suspension
In the ball mill stirred, the composite mixture of 20 weight portion is pulverized and adds 10 parts per weight dispersing agent and wetting agent and 70 weight parts waters or organic solvent, obtain reactive compound suspension in small, broken bits, dilute with water obtains stable reactive compound suspension, obtains the suspension that tetramycin complex mass percentage content is 20% in this way.
G) gel formulation
In ball mill, the composite mixture of 20 weight portion, 10 parts per weight dispersing agent, 1 weight portion gelling agent and 70 weight parts waters or organic solvent grinding are obtained delicate suspensions, dilute with water is obtained the stabilized with mixture suspension that tetramycin complex mass percentage content is 20% in this way.
H) ultra low volume solvent
The composite mixture of 10 weight portion is dissolved in 90 weight parts organic solvent as in dimethylbenzene, obtains the product used without dilution that the composite mixture quality degree of tetramycin is 10%.
This tetramycin complex can be used for the mode controlling disease similar to other preparations, insect pest and weeds etc.Simultaneously compared with unitary agent, can controlling disease, insect pest and weeds better, play the effect of Synergistic.Both tetramycin complex can be used and also tetramycin and active substance can be used successively.
In the application of this tetramycin complex, may be used for for controlling plant diseases, insect pest and weeds, also for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
This tetramycin complex can be used as disease on the plant in control different season and plant part (fruit, flower, leaf, stem, stem tuber, root), insect pest, weeds, and also protective plant late growing stage part provides growth institute energy requirement from germ infringement and for plant.
The application target crop of the tetramycin complex of the present invention's protection generally includes but is not limited to following plants kind: cereal (wheat, barley, rye, oat, paddy rice, corn, Chinese sorghum and related species); Beet (sugar beet and fodder beet); The operatic circle, drupe and berry (kiwi fruit, strawberry, raspberry, gooseberry, fruit mulberry, fig, pomegranate, carambola, guava etc.); Leguminous plant (Kidney bean, little inclined beans, pea, soybean, red bean); Oil plants (rape, shepherd's purse, opium poppy, olive, sunflower, coconut, castor oil plant, cocoa bean, peanut); Mellon plant (pumpkin, cucumber, muskmelon, balsam pear, wax gourd, watermelon); Fibre plant (cotton, flax, hemp, jute); Citrus fruit (orange, lemon, glucose shaddock, bar tangerine); Vegetables (spinach, asparagus lettuce, asparagus, wild cabbage, carrot, onion, tomato, potato, red pepper); Lauraceae (avocado, Chinese cassia tree, camphor) or such as tobacco, nut, coffee, eggplant, sugarcane, tea, pepper, comprise grape, banana, the vining plant of grass roots and natural rubber plant and ornamental plants (flowers, shrub, broad-leaved tree and evergreen plant) etc.It should be noted that, this inventory does not represent any restriction.
Tetramycin complex of the present invention also can be used for control industrial materials fungal infection (especially by mould microbial), bacterium or insect pest, and safeguard industries material infects from fungal attack with at this fungal infection rear reduction occurring or eradicates industrial materials.Industrial materials include but not limited to organic and inorganic material timber, paper, leather, natural or synthetic fibers and composite, such as particle board, plywood, wallboard etc.The present invention can be applied to this material with the consumption effectively suppressed.
Composite mixture of the present invention is used for the Prevention and Curation that disease, worm, crop smothering occur in plant, seed, soil, region, material and spatial dimension, simultaneously for plant provides growth institute energy requirement.
In use, its application form is this tetramycin complex: any one in preparaton, spray solution, emulsion, paste, oil.Type of service depends on and is specifically intended to object; Under certain conditions, should guarantee that tetramycin complex is meticulous and distribute equably.
In certain embodiments, when the application form of tetramycin complex is preparaton, tetramycin complex and solvent and/or carrier and/or emulsifier and/or the blended rear use of surfactant.
In certain embodiments, when tetramycin complex and the blended rear use of solvent, solvent comprise following one or more: water, arsol (as toluene, dimethylbenzene), paraffin (as mineral oil fractions), alcohols (as methyl alcohol, butanols, amylalcohol, benzylalcohol), ketone (as cyclohexanone, gamma-butyrolacton), pyrrolidones (NMP, NOP), acetic acid esters (ethylene acetate), dihydroxylic alcohols, fatty acid dimethylamides, fatty acid and fatty acid ester.
In certain embodiments, when tetramycin complex and the blended rear use of emulsifier, emulsifier comprise following one or more: nonionic and anion emulsifier (as polyoxyethylene aliphatic alcohol ether, alkylsulfonate and arylsulphonate);
In certain embodiments, when tetramycin complex and the blended rear use of surfactant, surfactant comprise following one or more: lignosulphonic acid, naphthalene sulfonic acids, phenolsulfonic acid, the alkali metal salt of dibutyl naphthalenesulfonic acid, alkali salt, ammonium salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, aliphatic alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, the condensation product of naphthalene or naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, alkyl phenyl polyglycol ether, tributyl phenyl polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, alcohol and fatty alcohol/ethylene oxide condensation product, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylation polyoxypropylene, laruyl alcohol polyglycol ether acetal, sorbitol ester, lignin sulfite waste liquor, methylcellulose.
When tetramycin complex acts on plant seed, the mode of preparaton can be made into, except above-mentioned each blending constituent, can also mix with adhesive and/or gelling agent and/or colouring agent.
When the application form of tetramycin complex is spray solution, emulsion, paste, oil, the material of dispersion is that mid-boiling point arrives high boiling mineral oil fractions, as kerosene or diesel oil, in addition the oil of coal tar and plant or animal origin is also had, aliphatic series, ring-type and aromatic hydrocarbon, such as toluene, dimethylbenzene, paraffin, tetrahydronaphthalene, alkylated naphthalene or derivatives thereof, methyl alcohol, ethanol, propyl alcohol, butanols, cyclohexanol, cyclohexanone, isophorone, intensive polar solvent, such as dimethyl sulfoxide (DMSO), 1-METHYLPYRROLIDONE and water.
Tetramycin complex adopts moisture type of service to make emulsion concentrates, paste by adding water in the application.For preparation emulsion, paste or oil dispersion, can by wetting agent, thickener, dispersant or emulsifier by this material directly or to be dissolved in after in oil or solvent homogenizing in water.But, the concentrate that is made up of composite mixture, wetting agent, thickener, dispersant or emulsifier and suitable solvent or oil can also be prepared and this concentrate is suitable for dilute with water.
In certain embodiments, tetramycin complex can be used for controlling disease comprise following one or more: fungal disease, bacterial disease, virus disease, nematodosis.
When tetramycin complex is for preventing and treating fungal disease, fungal disease comprise following one or more: Deuteromycotina fungi, Basidiomycotina fungi, Ascomycotina fungi, Zygomycotina fungi, Mastigomycotina fungi;
When tetramycin complex is for preventing and treating bacterial disease, bacterial disease comprise following one or more: pseudomonas, bacillus;
When tetramycin complex is used for preventing and treating virus disease, virus disease comprise following one or more: double-stranded DNA virus, single-stranded DNA viruses, diplornavirus, single strand RNA virus;
When tetramycin complex be used for pest control time, insect pest comprise following one or more: insect, mite class, snail, muroid;
When tetramycin complex be used for controlling weeds time, weeds comprise following one or more: grass, cyperus, broad-leaf weeds;
Provide the specific embodiment of several tetramycin complex below, should understand, these embodiments are only for further illustrating of the present invention, and be not used in and limit the scope of the invention, without departing from the idea case in the present invention described above, the various replacement made according to ordinary skill knowledge and customary means or change, all should comprise within the scope of the invention.
Example one tetramycin and procymidone composite
Wherein, active material component selects procymidone.Procymidone is interior absorption fungicide, has special efficacy to Botrytis and Sclerotinia fungi, can prevent and treat fruit tree, the gray mold of vegetable crop, stalk break, and fungi benzimidazole being produced to resistance is also effective.Use that rear protected effect is good, the lasting period is long, scab developmenting spread can be stoped.
The embodiment of the present invention is the method adopting Toxicity Determination and field trial to combine.First pass through Toxicity Determination, synergy ratio (SR) after clear and definite two kinds of medicaments are composite by a certain percentage, SR < 0.5 is antagonism, 0.5≤SR≤1.5 are summation action, SR > 1.5 is synergistic effect, on this basis, then carry out field trial.
Determine that after the effective inhibition concentration scope of each medicament, medicament establishes 5 dosage process respectively by active constituent content through prerun, if clear water contrast.Carry out with reference to " farm-chemical indoor determination test rule bactericide ", adopt mycelial growth rate method to measure medicament to the virulence of crop germ.Measure bacterium colony footpath by right-angled intersection method after 72h, calculate each process net growth, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter-5
Mycelial growth inhibition rate (%)=control group net growth-processed group net growth/control group net growth
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) converts logarithm value (x) to, try to achieve virulence regression equation (y=a+bx) with method of least squares, and calculate the EC50 value of often kind of medicament thus.Calculate two medicament different ratio synergies ratio (SR) according to Wadley method, SR < 0.5 is antagonism, and 0.5≤SR≤1.5 are summation action simultaneously, and SR > 1.5 is synergistic effect.
Computing formula is as follows:
SR＝EC
50(th)/EC
50(ob)
Wherein: a, b are respectively tetramycin and the ratio of procymidone shared by combination; A is tetramycin, and B is procymidone.
Tetramycin and procymidone composite to cucumber downy mildew Toxicity Determination.
Experimental scheme: through preliminary experiment determination tetramycin, effective inhibition concentration scope of procymidone and the two different ratio mixture.
Toxicity test result
The composite toxicity test analysis of results table to cucumber downy mildew of table 10.15% tetramycin, procymidone
As shown in Table 1, tetramycin, the EC50 of procymidone to cucumber downy mildew is respectively 2.57mg/L and 0.51mg/L.Tetramycin, when procymidone joins 80: 1 to 1: 80, synergy ratio SR is all greater than 1.5, illustrate that both tetramycin procymidones mixture in 80: 1 to 1: 80 scopes all shows synergistic effect, especially when the proportioning of the two is 20: 1 to 1: 10 time, synergistic effect is more obviously given prominence to, and wherein when tetramycin procymidone weight ratio is 10: 1, synergy ratio SR is maximum, and synergistic effect is the most obvious.
For tetramycin complex-42% tetramycin procymidone
Formula: tetramycin 40%, procymidone 2%, 3-methyl cyclohexanol ketenes 5%, diethylene glycol (DEG) 6%, xanthans 3%, fatty alcohol-polyoxyethylene ether 5%, aryl phenol polyoxyethylene succinate sulfonate 4%, silicone 0.5%, deionized water supplies 100%, obtained 42% tetramycin procymidone microemulsion.
Tetramycin, procymidone and the 42% tetramycin procymidone compound control fresh kidney beans gray mold test of pesticide effectiveness thereof
This experimental establishment is in Chaoyang County, Chaoyang City, and test medicine is researched and developed by Liaoning Wei Ke bio-engineering corporation and provided, contrast medicament 0.3% tetramycin aqua, 50% procymidone wetting powder (commercial).
The investigation fresh kidney beans gray mold state of an illness before medicine, in the first time dispenser of state of an illness initial stage, dispenser in every 7 days once, is total to dispenser 2 times.Within after second time dispenser 3 days, 7 days, 14 days, investigate disease index respectively and calculate preventive effect.Experimental result is as follows:
Table 2 tetramycin, procymidone and the compound control fresh kidney beans gray mold test of pesticide effectiveness thereof
Thus table can find out, tetramycin and procymidone composite after can effectively prevent and treat fresh kidney beans gray mold, control efficiency is better than the preventive effect of single dose, and the preventive effect phase is long.In test scope of medication, target crop is had no adverse effects.
After draw through tests different local throughout the country, tetramycin and procymidone, chlozolinate, sclex, isopropyl are fixed, the preventive effect of the Common Diseases such as the composite rear powdery mildew to all kinds of crop such as myclozolin, vinclozolin, fusarium wilt, downy mildew, gray mold, epidemic disease, anthracnose, ring spot, banded sclerotial blight, spot defoliation, scab, brown rot, leaf blight, rust is all more than 95%, be better than single dose preventive effect, synergistic effect is obvious.
Example two tetramycin and azoxystrobin compound
Wherein, active material component selects Fluoxastrobin
Embodiment 2-1 preparation method: 18% tetramycin Fluoxastrobin missible oil:
Take 6% tetramycin, 12% Fluoxastrobin, 4% agriculture breast 500# (calcium dodecyl benzene sulfonate), 3%Span-60# (sorbitan monostearate), 3% agriculture breast 2201#, 2% triphenyl phosphite, dimethylbenzene mends 100%.Fully dissolve after above-mentioned raw materials mixing, the obtained 18% tetramycin Fluoxastrobin missible oil of mixing.
Embodiment 2-2 preparation method: 24% tetramycin Fluoxastrobin suspending agent:
Take 4% tetramycin, 20% Fluoxastrobin, 3% agriculture breast 600,3% agriculture breast 2201,3% lignosulfonates, 0.2% xanthans, 6% ethylene glycol, 0.5% silicone defoaming agent, add deionized water and mend to 100%.After above-mentioned raw materials is mixed, through the obtained 24% tetramycin Fluoxastrobin suspending agent of high speed shear emulsification.
Embodiment 2-3 preparation method: 12% tetramycin azoxystrobin microemulsion:
Take 3% tetramycin, 9% Fluoxastrobin, 6% emulsifier if agriculture breast 500# (calcium dodecyl benzene sulfonate), 2% agriculture breast 2201#, 3% agriculture breast 600#, 1% methyl alcohol, 6% dimethylbenzene, 1.2% epoxychloropropane, 0.4% aceticanhydride, deionized water benefit are to 100%, above-mentioned raw materials is fully dissolved the obtained 12% tetramycin azoxystrobin microemulsion of mixing.
Assays Example 2-1: to the indoor colony growth inhibition test of rice sheath blight disease.
Test method is carried out with reference to Wu Wenjun " chemical protection of plant experimental technique introduction " (1993).The tetramycin mother liquor of 15%, the former medicinal acetone of 95.4% Fluoxastrobin, Tween-80 are made 1.0 μ g/mL mother liquors, utilize that mother liquor becomes Fluoxastrobin, tetramycin mass ratio is respectively 3:1 ~ 5:1, gradient be respectively 0.01 μ g/mL, 0.02 μ g/mL, 0.04 μ g/mL, 0.08 μ g/mL PDA dull and stereotyped.The concrete data of result of the test are in table 3.
Table 3 Fluoxastrobin, tetramycin indoor antibacterial test data
Calculate the toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture according to the abundant method of Sun Yun, when CTC≤80 are antagonism, 80 ﹤ CTC ﹤ 120 are summation action, and CTC >=120 are synergistic effect.
Found by indoor bacterium colony Developing restraint test, Fluoxastrobin, tetramycin long all have synergy inhibitory action according to composite rear to be born to Rhizoctonia solani Kuhn of the mass ratio of 3:1 ~ 5:1, wherein obvious with tetramycin, Fluoxastrobin 1:2 synergy, co-toxicity coefficient is 234.57, the two compound synergic is remarkable, more effectively can suppress the expansion of bacterium colony.
Assays Example 2-2: the field control effectiveness test of the water prevention rice sheath blight.
Reagent agent and dispenser dosage: (1) 24% tetramycin Fluoxastrobin suspending agent (preparation method embodiment 8-2), mu 40 grams, preparation; (2) 12% tetramycin azoxystrobin microemulsion (preparation method embodiment 2-3), mu 80 grams, preparation.
Contrast medicament and dispenser dosage: (3) 1.5% tetramycin aquas, mu 40 grams, preparation; (4) 10% Fluoxastrobin wetting powders, mu 20 grams, preparation,
Spraying time and application method: the dispenser of rice sheath blight disease their early stage, is watered even spraying.
The field control effectiveness test result of the table 4 water prevention rice sheath blight
| Reagent agent | Preparation usage amount (g/ mu) | 7 days preventive effects (%) after medicine | 14 days preventive effects (%) after medicine |
| 1 | 40 | 74.3 | 81.3 |
| 2 | 80 | 73.8 | 80.7 |
| 3 | 40 | 66.5 | 72.5 |
| 4 | 20 | 61.5 | 71.9 |
Result of the test: in table 4.As can be seen from the test results: after dispenser 7 days, tetramycin of the present invention and Fluoxastrobin mixture composition to rice sheath blight disease preventive effect all more than 70%, significantly better than the single dose of contrast; After dispenser 14 days, tetramycin of the present invention and Fluoxastrobin mixture composition to rice sheath blight disease preventive effect all more than 80%, also significantly better than the single dose of contrast; In process of the test, each medicament is showed no poisoning generation, to crop safety.Illustrate that tetramycin of the present invention and Fluoxastrobin mixture composition are better than single dose, improve actual control efficiency, reduce dosage, reduce costs, reduce medicament to the adverse effect of environment, delay the generation of disease drug resistance, agricultural synthesis is administered and has great significance.
Example three tetramycin and chlorine worm benzamide composite
Wherein, active material component selects chlorine worm benzamide.Tetramycin chlorine worm benzamide compound can effectively prevent and treat rice blast and rice leaf roller, facilitates peasant to use.Use tetramycin chlorine worm benzamide compound, while treatment rice blast, effectively prevent rice leaf roller, reduce the medicament that the later stage uses control rice leaf roller; Even if two kinds of diseases occur simultaneously, complex preparation also has good therapeutic action.
The preparation of suspending agent:
According to the proportion requirement of formula, add wetting agent, dispersant, former medicine, bleeding agent, defoamer, thickener, antifreezing agent respectively, sleeping mixes, be fully ground to product average grain diameter and be less than 2 microns, obtain suspending agent product, see example below.
The weight ratio of each component in embodiment 3-1 suspending agent
Chlorine worm benzamide 6%, tetramycin 18%, sodium dodecylbenzenesulfonate 7%, JFC4.5%, polyvinyl alcohol 2.5%, NNO 6%, xanthans 0.5%, propane diols 1%, S-29,0.5%, water complements to 100%.
The weight ratio of each component in example 3-2 suspending agent
Chlorine worm benzamide 29%, tetramycin 20%, Nonyl pheno base ether 5.5%, JFC4.5%, polyvinyl alcohol 2.5%, NNO8%, xanthans 0.5%, S-290.5%, water complements to 100%.
Field trial embodiment:
In July, 2008, paddy rice carries out the test of pesticide effectiveness, it is the occurrent time of rice blast during test, blade there is scab, the medium generation of rice leaf roller, medicament selects 20% chlorine worm benzamide suspending agent and 0.3% tetramycin aqua, interval second time dispenser afterwards in 10 days after first time dispenser, 15 days investigation insecticidal effects and bactericidal effect after second time dispenser.
The investigation method of rice blast is: 5 fixed point observations are selected in each community, often two clumps of paddy rice, and before dispenser, investigation disease refers to, within after last dispenser 15 days, investigates disease leaf rate, disease index, calculates the control efficiency of each medicament.
Rice blast grade scale:
0 grade: anosis
1 grade: leaf spot lesion is less than 5, and length is less than 1cm;
3 grades: leaf spot lesion 6-10, part scab length is greater than 1cm;
5 grades: leaf spot lesion 11-25, part scab is linked to be sheet, accounts for blade area 10-25%;
7 grades: leaf spot lesion more than 26, scab is linked to be sheet, accounts for blade area 26-50%;
9 grades: scab is linked to be sheet, account for blade area more than 50% or all withered.
Disease index=100 × ∑ (the sick number of sheets at different levels × progression relatively)/(investigating total number of sheets × total progression)
Control efficiency (%)=[1-(the sick index of the sick index/CK1 × Pt0 of CK0 × Pt1)] × 100
CK0 is the disease index of the front check plot of dispenser; Ck1 is the disease index of check plot after dispenser;
Pt0 is the disease index before treatment region dispenser; Pt1 is the disease index of dispenser post processing zone;
The investigation method of rice leaf roller: 15 days investigation leaf roll numbers after dispenser the last time, calculate control efficiency.
Leaf roll rate (%)=(investigation leaf roll number/investigate total leaf tree) × 100
Control efficiency (%)=[(blank district leaf roll rate-treatment region leaf roll rate)/blank district leaf roll rate] × 100
Table 5 chlorine worm benzamide, tetramycin test of pesticide effectiveness result
From test, after chlorine worm benzamide and tetramycin are composite, have good control efficiency to rice blast and rice leaf roller, after dispenser for the second time 15 days to the preventive effect of rice blast and rice leaf roller all more than 90%.Peasant's a drug can effectively preventing rice blast and rice leaf roller, because of chlorine worm benzamide and tetramycin all have efficient in absorption conduction, can lasting period of significant prolongation medicament, reduce times for spraying, reduce planting cost.Find in test, complex preparation, except having good control efficiency to rice leaf roller, also has rice fulgorid and certain doublely controls effect, rice fulgorid and propagate paddy rice multiple diseases, reduces rice fulgorid insect population radix, is also conducive to the control of other disease of later stage.
Example four tetramycin and carboxin composite
Wherein, active material component selects carboxin, its structural formula following (I), chemical name: 5,6-dihydro-2-Methyl-N-phenyl-Isosorbide-5-Nitrae-oxathiene-3-formamide, a kind of heterocyclic nitrogen-group fungicide with systemic action, it can infiltrate the seed of rudiment and kill intraseminal germ, has higher systemic activity to load guiding principle fungi.
Embodiment 4-1: tetramycin and carboxin compound measure the indoor harmacological effect of cotton seedling blight.
Subjects: cotton rhizoctonia solani
Test method: suppress disease fungus mycelial growth test Plating to carry out with reference to People's Republic of China (PRC) farm-chemical indoor determination test rule NYT 1156.2-2006.Cotton rhizoctonia solani cultivation temperature is 28 DEG C, adopts medium to be PDA medium.
Connect bacterium to measure after 1 week, statistical analysis, result of the test is see table 7.
Table 6 tetramycin and carboxin compound are to cotton rhizoctonia solani measurement result
Result of the test shows, 20:1 ~ 1:1 is composite by weight for tetramycin and carboxin, and when preventing and treating cotton rhizoctonia solani, co-toxicity coefficient is all more than 120, has synergistic effect; Wherein, when proportioning is 10:1, co-toxicity coefficient is the highest.
Embodiment 4-2:21% tetramycin carboxin suspending agent
Formula: tetramycin 20%, carboxin 1%, methyl naphthalene sulfonate formaldehyde condensate 10%, xanthans 0.4%, bentonite 1%, glycerine 5%, water complements to 100%.
Embodiment 4-3:16.5% tetramycin carboxin suspending agent seed coat agent
Composition: tetramycin 15%, carboxin 1.5%, rose-red 0.3%, methyl naphthalene sulfonate formaldehyde condensate 10%, xanthans 0.4%, bentonite 1%, glycerine 5%, water complements to 100%.
Embodiment 4-4:12% tetramycin carboxin suspending agent seed coat agent
Composition: tetramycin 10%, carboxin 2%, rose-red 0.3%, methyl naphthalene sulfonate formaldehyde condensate 10%, xanthans 0.4%, bentonite 1%, glycerine 5%, water complements to 100%.
By the pesticidal preparations seed dressing control cotton seedling blight field trial that above-described embodiment is obtained.Concrete using method is: above-mentioned each preparation is blended into appropriate water and stirs, more evenly mixes sowing on wheat seed and after drying in the shade.This test medicine consumption establishes two concentration for the treatment of respectively according to the difference of each composition.
Result of the test is as table 7.
Table 7 tetramycin and the control effect testing of carboxin complex preparation seed dressing to cotton seedling blight
Note: the significance of difference (p < 0.05) representing variance analysis with a, b, the c after column data.
Field efficacy result display in table, tetramycin carboxin composition has good control efficiency to cotton seedling blight, and under same dose, preventive effect is better than single dose, and when wherein tetramycin carboxin proportioning is 10: 1, activity is the highest.
Conclusion: above-described embodiment shows, tetramycin carboxin is more alone after combining to be compared, and is significantly improved, shows obvious synergistic effect to the control efficiency of cotton seedling blight.Wherein the highest with synergistic effect during tetramycin carboxin 10: 1.
Example the May 4th mycin and dimethomorph composite
Wherein, active material component selects dimethomorph.Tetramycin, the former medicine of dimethomorph are made 20% suspending agent respectively and carried out indoor pot experiment.Test method is carried out with reference to Wu Wenjun " chemical protection of plant experimental technique introduction " (1993).According to requirement of experiment, make single dose respectively, and the type of respective quality ratio.The concrete data of result of the test are in table 8.
Table 8 tetramycin and dimethomorph indoor pot experiment data.
Calculate the toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture according to the abundant method of Sun Yun, when CTC≤80 are antagonism, 80 ﹤ CTC ﹤ 120 are summation action, and CTC >=120 are synergistic effect.
Found by the test of indoor bacterium colony Developing restraint, tetramycin and dimethomorph all have synergistic effect to cucumber downy mildew afterwards according to the ratio of 3:1 ~ 1:5 is composite, and wherein obvious with 1:3 synergy, co-toxicity coefficient is 227.68, and the two compound synergic is remarkable.
20% tetramycin dimethomorph suspending agent:
0.3% tetramycin 4%, dimethomorph 16%, methyl naphthalene sulfonate formaldehyde condensate (dispersant) 5%, 0203B (surfactant) 6%, white carbon (filler) 5%, aluminium-magnesium silicate (suspending agent) 0.4%, ethylene glycol (antifreezing agent) 3%, tributyl phosphate (defoamer) 1.5%, deionized water complements to 100%.Above-mentioned formula is pulverized in proportion in advance, then adds in sand mill and grind, the obtained 20% tetramycin dimethomorph suspending agent of allotment after high speed shear mixing.
This formula control cucumber downy mildew is by 2000 times of dilution sprayings, and after medicine, 7 days control efficiency are 91.3%.6% tetramycin aqua is by 1500 times of dilution sprayings, and after medicine, 7 days control efficiency are that 81.2%, 40% dimethomorph water dispersion granula dilutes spraying by 1500 times, and after medicine, 7 days control efficiency are 69.7%.Test shows that 20% tetramycin dimethomorph suspending agent has good control efficiency to cucumber downy mildew, is better than corresponding single dose.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a tetramycin complex, is characterized in that, comprising: tetramycin and active substance; Described active substance is amide-type, described amide-type comprise following one or more: benzamides, dicarboximide class, benzamide type, strobilurins, N-halogenmethylthio-dicarboximide class, dimethomorph.
2. tetramycin complex according to claim 1, is characterized in that, the mass concentration of described tetramycin in described tetramycin complex is 0.15-99%.
3. tetramycin complex according to claim 1, is characterized in that, described tetramycin complex is any one or more in aqua, dispersible agent, missible oil, microemulsion, emulsion, suspension, gel formulation, ultra low volume solvent.
4. the tetramycin complex according to any one of claim 1-3, it is characterized in that, when described active substance comprises benzamides, described benzamides comprise following one or more: M 9834, metalaxyl, R-metalaxyl, Wakil, chlorine worm benzamide;
When described active substance comprises dicarboximide class, described dicarboximide class comprise following one or more: chlozolinate, sclex, isopropyl are fixed, myclozolin, procymidone, vinclozolin;
When described active substance comprises benzamide type, described benzamide type comprise following one or more: carboxin, first furan anilide, flutolanil, mebenil, oxycarboxin or thifluzamide, alachlor, Acetochlor, the third careless amine;
When described active substance comprises strobilurins, described strobilurins comprise following one or more: nitrile Fluoxastrobin, imines bacterium, fork amine benzamide, pyraclostrobin, ZEN 90160,2 [(2-trifluoromethyl)-pyridine-6-base oxygen ylmethyl]-3-methoxy group-methyl acrylate, Lonster, Flumiclorac pentyl, oxime bacterium ester;
When described active substance comprises N-halogenmethylthio-dicarboximide class, described N-halogenmethylthio-dicarboximide class comprise following one or more: difoltan, captan, Euparen, fluoromide, folpet or Tolylfluanid.
5. the application of the tetramycin complex described in any one of claim 1-4, is characterized in that, for controlling plant diseases, insect pest, weeds; And/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
6. the application of tetramycin complex according to claim 5, is characterized in that, the application form of described tetramycin complex is: any one in preparaton, spray solution, emulsion, paste, oil.
7. the application of tetramycin complex according to claim 6, it is characterized in that, when the application form of described tetramycin complex is preparaton, described tetramycin complex and solvent and/or carrier and/or emulsifier and/or the blended rear use of surfactant.
8. the application of tetramycin complex according to claim 7, is characterized in that,
When described tetramycin complex and the blended rear use of solvent, described solvent comprise following one or more: water, arsol, paraffin, alcohols, ketone, pyrrolidones, acetic acid esters, dihydroxylic alcohols, fatty acid dimethylamides, fatty acid and fatty acid ester;
When described tetramycin complex and the blended rear use of carrier, described carrier comprise following one or more: the kaolin ground, the clay ground, the talcum ground, the chalk ground, the silica ground, the silicate ground;
When described tetramycin complex and the blended rear use of emulsifier, described emulsifier comprise following one or more: nonionic and anion emulsifier;
When described tetramycin complex and the blended rear use of surfactant, described surfactant comprise following one or more: lignosulphonic acid, naphthalene sulfonic acids, phenolsulfonic acid, the alkali metal salt of dibutyl naphthalenesulfonic acid, alkali salt, ammonium salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, aliphatic alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, the condensation product of naphthalene or naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, alkyl phenyl polyglycol ether, tributyl phenyl polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, alcohol and fatty alcohol/ethylene oxide condensation product, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylation polyoxypropylene, laruyl alcohol polyglycol ether acetal, sorbitol ester, lignin sulfite waste liquor, methylcellulose.
9. the application of tetramycin complex according to claim 5, is characterized in that, for described disease comprise following one or more: fungal disease, bacterial disease, virus disease, nematodosis.
10. the application of tetramycin complex according to claim 9, is characterized in that,
When described tetramycin complex is for preventing and treating fungal disease, described fungal disease comprise following one or more: Deuteromycotina fungi, Basidiomycotina fungi, Ascomycotina fungi, Zygomycotina fungi, Mastigomycotina fungi;
When described tetramycin complex is for preventing and treating bacterial disease, described bacterial disease comprise following one or more: pseudomonas, bacillus;
When described tetramycin complex is used for preventing and treating virus disease, described virus disease comprise following one or more: double-stranded DNA virus, single-stranded DNA viruses, diplornavirus, single strand RNA virus;
When described tetramycin complex be used for pest control time, described insect pest comprise following one or more: insect, mite class, snail, muroid;
When described tetramycin complex be used for controlling weeds time, described weeds comprise following one or more: grass, cyperus, broad-leaf weeds.
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| CN105638769A (en) * | 2016-04-13 | 2016-06-08 | 储志强 | Method for preparing pesticide |
| CN113367146A (en) * | 2021-05-11 | 2021-09-10 | 贵州省亚热带作物研究所 | Compound bactericidal preparation for preventing and treating coix seed leaf blight and use method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696652A (en) * | 2012-06-29 | 2012-10-03 | 青岛星牌作物科学有限公司 | Sterilizing composite for controlling pyricularia oryzae |
| CN103300024A (en) * | 2013-06-19 | 2013-09-18 | 北京燕化永乐农药有限公司 | Sterilization composition containing tetramycin |
-
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696652A (en) * | 2012-06-29 | 2012-10-03 | 青岛星牌作物科学有限公司 | Sterilizing composite for controlling pyricularia oryzae |
| CN103300024A (en) * | 2013-06-19 | 2013-09-18 | 北京燕化永乐农药有限公司 | Sterilization composition containing tetramycin |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638769A (en) * | 2016-04-13 | 2016-06-08 | 储志强 | Method for preparing pesticide |
| CN113367146A (en) * | 2021-05-11 | 2021-09-10 | 贵州省亚热带作物研究所 | Compound bactericidal preparation for preventing and treating coix seed leaf blight and use method thereof |
| CN113367146B (en) * | 2021-05-11 | 2022-08-05 | 贵州省亚热带作物研究所 | Compound bactericidal preparation for preventing and treating coix seed leaf blight and use method thereof |
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