CN102726394A - Sterilization composition containing propamidine and chitosan oligosaccharides - Google Patents
Sterilization composition containing propamidine and chitosan oligosaccharides Download PDFInfo
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- CN102726394A CN102726394A CN2012102081517A CN201210208151A CN102726394A CN 102726394 A CN102726394 A CN 102726394A CN 2012102081517 A CN2012102081517 A CN 2012102081517A CN 201210208151 A CN201210208151 A CN 201210208151A CN 102726394 A CN102726394 A CN 102726394A
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Abstract
The invention discloses a sterilization composition containing propamidine and chitosan oligosaccharides, wherein the mass ratio of the propamidine to the chitosan oligosaccharides is 4:1-8:1. The composition can be missible oil, a microemulsion, a soluble liquid or an aqua, can be used for controlling fruit or vegetable crop diseases, and can effectively control cucumber fusarium wilt, cucumber downy mildew, tomato gray mold and the like. The composition has the advantages of environmental protection, wide application range, low cost and good effect.
Description
Technical field
The present invention relates to pesticidal preparations and preparation thereof, be specifically related to a kind of bactericidal composition that contains propamidine and chitosan oligosaccharide, be applicable in fungal diseases of plants such as control cucumber fusarium axysporum, cucumber downy mildew, graw mold of tomato.
Background technology
1, propamidine
Propamidine (Propamidine), chemical name are 1, and 3-two (4-amidino groups phenoxy group) propane is systemic fungicide, can in plant corpus, absorb to distribute and metabolism, have protection and treatment double effects.This medicine is mainly used in the plurality of plant diseases of vegetables, fruit tree and economic crops in control field and the booth.According to China's pesticide toxicity grading criteria, the propamidine bactericide belongs to the low toxicity bactericide, to environmental organism safety.
According to the data-searching that the applicant did; The Mixed Pharmacy that with the propamidine is the basis at present mainly contains: Zhang Qiufang etc. have applied for the patent of " a kind of bactericidal composition that contains propamidine and fourth pyrrole morpholine "; Publication number is CN101965843A; Its method is that the weight ratio of propamidine and fourth pyrrole morpholine is 1 ~ 60:80 ~ 1, and the effective active composition adds auxiliary agent and excipients is processed wetting powder, water dispersible granules, suspending agent, can effectively prevent and treat the gray mold, downy mildew, eqpidemic disease of gourd, fruit and vegetable etc.; Yang Shouhai etc. have applied for the patent of " a kind of bactericidal composition and application thereof that contains propamidine and fine bacterium azoles "; Publication number is CN1930972A; The weight ratio of propamidine and fine bacterium azoles is 5 ~ 45:1 ~ 40; The effective active composition adds auxiliary agent and excipients is re-dubbed pulvis, wetting powder, suspending agent, missible oil or granule, and said composition is used to prevent and treat cereal class, melon and fruit class and vegetable crop disease; Yang Shouhai etc. have applied for the patent of " a kind of bactericidal composition and application thereof that contains propamidine and thiophanate-methyl "; Publication number is CN1930980A; The weight ratio of propamidine and thiophanate-methyl is 5 ~ 30:1 ~ 60 in the said composition, and it can be used for preventing and treating cereal class, melon and fruit class and vegetable crop disease; Guo Shenghu etc. have applied for the patent of " preparation method and the application thereof of a kind of natural pond liquid composite sterilizer liquid "; Publication number is CN102047913A; This natural pond liquid compound disinfectant is composited by the constant weight percentage composition by bactericidal composition and the natural pond liquid for preparing, and wherein the component of bactericidal composition and weight percentage are: propamidine 0.1% ~ 2%, metalaxyl 5% ~ 10%; Solvent and insecticides adjuvant 94.9% ~ 88%; Said bactericidal composition can be processed missible oil, aqueous emulsion or soluble liquid, with the bactericidal composition for preparing and natural pond liquid according to percentage by weight: natural pond liquid 99.5 ~ 99.7%, bactericidal composition 0.3 ~ 0.5% mix homogenize and get final product.
2, chitosan oligosaccharide
Amino-oligosaccharide is also referred to as agriculture special-purpose chitosan oligosaccharide.Chitosan oligosaccharide is as a kind of exciton, inducing plant disease resistance effectively, and enhancement of plant is to the defence capability of damage by disease and insect.Chitosan oligosaccharide is used characteristics such as have trace (PPM level), efficient, low-cost, nuisanceless on agricultural, significant to China's agricultural sustainability development.
Making a general survey of above-mentioned patent, mostly is to carry out compositely with propamidine and existing bactericide, finds in early stage practice that propamidine and chitosan oligosaccharide are composite and has a synergistic effect.Also not seeing at present has propamidine and chitosan oligosaccharide is composite or with propamidine, the composite bactericide that is mixed that is the basis of chitosan oligosaccharide.
Summary of the invention
The objective of the invention is to, utilize propamidine and chitosan oligosaccharide to be primary raw material, process a kind of bactericidal composition that contains propamidine and chitosan oligosaccharide, and add other auxiliary agent on this basis and process the bactericide that is mixed.
In order to realize above-mentioned task, the present invention adopts following technical solution:
A kind of bactericidal composition that contains propamidine and chitosan oligosaccharide
A kind of bactericidal composition that contains propamidine and chitosan oligosaccharide is characterized in that, the active ingredient of said composition is propamidine and chitosan oligosaccharide, and the mass ratio of said propamidine and chitosan oligosaccharide is 4:1 ~ 8:1, and the preparation form is missible oil, microemulsion, soluble liquid or aqua; Wherein:
Missible oil is formulated according to percentage by weight by following component; Propamidine: 0.01%～10%, chitosan oligosaccharide: 0.01%～5%, emulsifier: 5%～20%, synergist: 1%～10%, solvent is mended to 100%;
Microemulsion is formulated according to percentage by weight by following component; Propamidine: 0.01%～10%, chitosan oligosaccharide: 0.01～5%, emulsifier: 10%～40%, synergist: 1%～10%; Cosolvent: 5～40%; Antifoaming agent: 0.02%, water is mended to 100%;
Soluble liquid is formulated according to percentage by weight by following component; Propamidine: 0.01%～10%, chitosan oligosaccharide: 0.01～5%, emulsifier: 10%～40%, synergist: 1%～10%, solvent is mended to 100%.
Aqua is formulated according to percentage by weight by following component; Propamidine: 0.01%～10%, chitosan oligosaccharide: 0.01%～5%, emulsifier: 10%～40%, synergist: 1%～10%, cosolvent: 1%～40%; Antifoaming agent: 0.02%, water is mended to 100%.
Emulsifier, solvent and synergist used in the said missible oil are respectively:
Emulsifier: one or more mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Solvent: the mixture of one or more in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, the N-Methyl pyrrolidone;
Synergist: the mixture of one or more among oil-soluble azone, AEO, the fast T.
Emulsifier, synergist, cosolvent and antifoaming agent used in the said microemulsion are respectively:
Emulsifier: one or more mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Synergist: the mixture of one or more among oil-soluble azone, AEO, the fast T;
Cosolvent: the mixture of one or more in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, N-Methyl pyrrolidone, the pyroligneous liquor,
Antifoaming agent is selected silicone defoaming agent.
Used emulsifier, synergist and the solvent of said soluble liquid is respectively:
Emulsifier: one or more mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Synergist: the mixture of one or more among oil-soluble azone, AEO, the fast T;
Solvent: the mixture of one or more in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, the N-Methyl pyrrolidone, in the pyroligneous liquor, acetate, water.
Used emulsifier, synergist, cosolvent and the antifoaming agent of said aqua is respectively:
Emulsifier: one or more mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Cosolvent: the mixture of one or more in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, the N-Methyl pyrrolidone, in the pyroligneous liquor, acetate;
Synergist: the mixture of one or more among oil-soluble azone, AEO, the fast T; Solvent is a water.
Antifoaming agent is selected silicone defoaming agent.
The bactericidal composition that contains propamidine and chitosan oligosaccharide of the present invention; Applied widely, cost is low, effective, be environmentally friendly agricultural chemicals of new generation, can effectively prevent and treat various diseases; Comparatively outstanding to cucumber fusarium axysporum, cucumber downy mildew, graw mold of tomato effect, be a kind of novel agrochemical.
Embodiment
The preparation of indication of the present invention is meant the preparation that contains propamidine and chitosan oligosaccharide; The mass ratio of propamidine and chitosan oligosaccharide is 4:1 ~ 8:1; Its preparation method is with solvent propamidine and chitosan oligosaccharide to be dissolved, and adds auxiliary agent and processes missible oil, microemulsion, soluble liquid or aqua by the method for routine.
Through applicant's evidence, the present invention preparation contain propamidine and chitosan oligosaccharide bactericidal composition, remarkable to downy mildew, gray mold effect, bactericidal activity all has obvious synergistic effect than single agent and bactericidal composition.
Wherein, when described bactericidal composition was mixed with missible oil, described missible oil was formulated according to percentage by weight by following component: propamidine: 0.01%～10%, and chitosan oligosaccharide: 0.01～5%.Emulsifier: 5%～20%, synergist: 1%～10%, solvent is mended to 100%.
Described emulsifier is one or more a mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Solvent is one or more the mixture in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, the N-Methyl pyrrolidone;
Synergist is one or more the mixture among oil-soluble azone, AEO, the fast T.
When described bactericidal composition was mixed with microemulsion, described microemulsion was formulated according to percentage by weight by following component:
Propamidine: 0.01%～10%, chitosan oligosaccharide: 0.01～5%, emulsifier: 10%～40%, synergist: 1%～10%, cosolvent: 5%～40%, antifoaming agent: 0.02%, water is mended to 100%.
Described emulsifier is one or more a mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Cosolvent is one or more the mixture in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, N-Methyl pyrrolidone, the pyroligneous liquor;
Synergist is one or more the mixture among oil-soluble azone, AEO, the fast T;
Antifoaming agent is selected silicone defoaming agent.
When described bactericidal composition is mixed with soluble liquid, described formulated according to percentage by weight by following component:
Propamidine: 0.01%～10%, chitosan oligosaccharide: 0.01～5%, emulsifier: 10%～40%, synergist: 1%～10%, solvent is mended to 100%.
Described emulsifier is one or more a mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Solvent is one or more the mixture in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, N-Methyl pyrrolidone, pyroligneous liquor, acetate, the water;
Synergist is one or more the mixture among oil-soluble azone, AEO, the fast T.
When described bactericidal composition was mixed with aqua, described aqua was formulated according to percentage by weight by following component:
Propamidine: 0.01%～10%, chitosan oligosaccharide: 0.01%～5%, emulsifier: 10%～40%, synergist: 1%～10%, cosolvent: 1%～40%; Antifoaming agent: 0.02%, water is mended to 100%.
Described emulsifier is one or more a mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Cosolvent can be in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, the N-Methyl pyrrolidone, one or more the mixture in the pyroligneous liquor, acetate;
Synergist is one or more the mixture among oil-soluble azone, AEO, the fast T.
Antifoaming agent is selected silicone defoaming agent.
Microemulsion and aqua are medium with water, have reduced the use of organic solvent in a large number, have lowered the murder by poisoning to environment; And active ingredient exists with nanoscale, and dispersity is high, through adding suitable building component, has improved the active component effective rate of utilization greatly.
Therefore, in practical application, propamidine of the present invention and chitosan oligosaccharide bactericidal composition, preferably processing formulation is microemulsion and aqua.
In order better to understand essence of an invention, test the technology contents that specifies invention below in conjunction with embodiment with concrete, but the present invention is not limited to these embodiment.
The preparation of preparation embodiment 1:4.5% propamidine chitosan oligosaccharide (4:0.5) soluble liquid
Under 20 ℃ ~ 25 ℃; Get 4 parts of propamidines; 0.5 part chitosan oligosaccharide adds 30 parts of dimethyl sulfoxide (DMSO)s, 10 parts of methyl alcohol and 5 parts of cyclohexanone stirrings it is fully dissolved, and in said mixture, adds 10 parts of OP-10 successively then, surplus is a pyroligneous liquor; Stirred 10～30 minutes for 800～1000 rev/mins with mixing speed, can make 4.5% propamidine chitosan oligosaccharide soluble liquid.
The preparation of preparation embodiment 2:4.5% propamidine chitosan oligosaccharide (4:0.5) missible oil
Under 20 ~ 25 ℃; Get 4 parts of propamidines; 0.5 part chitosan oligosaccharide adds 60 parts of methyl alcohol stirrings it is fully dissolved, and in said mixture, adds 10 parts of OP-10 then successively, 0.2 part of oil-soluble azone, surplus are ethanol; Stirred 10～30 minutes for 800～1000 rev/mins with mixing speed, can make 4.5% propamidine chitosan oligosaccharide missible oil.
The preparation of preparation embodiment 3:2.5% propamidine chitosan oligosaccharide (2:0.5) microemulsion
Under 20 ℃ ~ 25 ℃; Get 2 parts of propamidines; 0.5 part chitosan oligosaccharide adds 30 parts of methyl alcohol stirrings it is fully dissolved, and in said mixture, adds 15 parts of OP-10 successively then, surplus is a water; Stirred 10～30 minutes for 800～1000 rev/mins with mixing speed, can make 2.5% propamidine chitosan oligosaccharide microemulsion.
The preparation of preparation embodiment 4:10% propamidine chitosan oligosaccharide (8:2) soluble liquid
Under 20 ~ 25 ℃; Get 8 parts of propamidines; 2 parts of chitosan oligosaccharides add 20 parts of N-Methyl pyrrolidone, 10 parts of dimethyl formamides stir it is fully dissolved, and in said mixture, adding 2 parts of 500#, 5 parts of 700#, 5 parts of NP-10,0.2 part of oil-soluble azone, surplus then successively is dimethyl sulfoxide (DMSO); Stirred 10～30 minutes for 800～1000 rev/mins with mixing speed, can make 10% propamidine chitosan oligosaccharide soluble liquid.
The results from pot experiment test of 1: four kind of propamidine chitosan oligosaccharide of test example complex preparation control cucumber fusarium axysporum
1, materials and methods
1.1 supply the reagent agent
2.5% propamidine chitosan oligosaccharide SL, 4.5% propamidine chitosan oligosaccharide SL, 4.5% propamidine chitosan oligosaccharide EC, 10% propamidine chitosan oligosaccharide SL, 50% carbendazim WP.
1.2 supply to study thing
Grind No. 4 in cucumber Tianjin, potted plant growth
1.3 controlling object
Cucumber fusarium axysporum (Fusarium oxysporum)
1.4 experimental scheme
Cucumber fusarium axysporum belongs to soil-borne disease, adopts root-pouring method when therefore handling.Cucumber seeds is preserved moisture under room temperature shading condition at warm bubbly water 4h then, treats that seed shows money or valuables one carries unintentionally the back sowing in flowerpot, and cucumber seedling begins test when growing 3 true leaves.The assay method of protective effect is around first perfusion liquid to the root system of plant to be measured, and manufacture-yellow cucurbit wilt bacterium spore suspension was with 107 spore/ml after high temperature, high humidity were preserved 48h, and the amount of 5ml zymotic fluid/strain is seeded in germ in the plant rhizosphere soil on every side.Therapeutic action is the first spore suspension of inoculation cucumber fusarium axysporum fungal pathogens, perfusion liquid again behind the 48h that preserves moisture.Every processing repetition 5 times, every basin 1 strain is checked incidence after cultivating 20d under the hot and humid condition.(reach 1998 in the side; Yue Dongxia etc. 2008; Cheng Zhihui etc. 2008)
1.5 investigation method
The incidence of cucumber seedling fusarium wilt is 5 grades by criteria:
0 grade: complete stool is anosis, does not show the disease symptom;
1 grade: complete stool has the 1/4 withered and morbidity with lower blade;
2 grades: complete stool has the withered morbidity of 1/4～1/2 blade;
3 grades: complete stool has 1/2 with the withered morbidity of blade;
4 grades: plant is due to illness withered.
Blank is the clear water contrast, and the medicament contrast is the carbendazol wettable powder of 1000 times of dilutions.
Disease index and control efficiency are calculated according to following formula:
2, result and analysis
With the cucumber fusarium axysporum is to supply the examination object, has carried out pot experiment to supplying four kinds of propamidine chitosan oligosaccharide complex preparations of examination, and concrete outcome is seen table 1.
Table 1: supply the reagent agent to cucumber fusarium axysporum protective effect results from pot experiment test
Annotate: above test data is 6 times a repetition mean value, uses Duncan ' s duncan's new multiple range method to analyze 5% significance level difference.
Can be known that by table 1 four kinds of propamidine chitosan oligosaccharide complex preparations have all shown higher pot experiment control efficiency, wherein 4.5% propamidine chitosan oligosaccharide complex preparation has shown the control efficiency that is superior to other preparation.4.5% propamidine chitosan oligosaccharide EC is in the cucumber fusarium axysporum protective effect test; Preventive effect is 53.79% behind 10d under 100 times of liquid of dilution; And this proportioning soluble liquid shows excellence in therapeutic action; Preventive effect is 52.20% behind the 10d, and significance difference opposite sex analytical table is bright, does not have the significance difference opposite sex with contrast medicament 80% carbendazim.And other proportioning preparation has also shown higher control efficiency.2: four kinds of propamidine chitosan oligosaccharides of test example complex preparation control graw mold of tomato field control effectiveness test
1, materials and methods
1.1 supply the reagent agent
2.5% propamidine chitosan oligosaccharide SL, 4.5% propamidine chitosan oligosaccharide SL, 4.5% propamidine chitosan oligosaccharide EC, 10% propamidine chitosan oligosaccharide SL (self-control), 40% execute good happy SC, German Ai Gefu company.
1.2 supply to study thing
Tomato (Lycopersicon esculentum), the green house plantation.
1.3 controlling object
Graw mold of tomato (Botrytis cinerea)
1.4 dosage
After confirming every sub-district water consumption, adopt double dilution method to carry out the soup configuration.
Every sub-district water consumption: 1.85L
1.5 investigation method
Respectively before for the first time spraying medicine, spray medicine for the second time before, for the third time spray medicine before, carried out the incidence investigation before the 4th spray medicine and behind the 4th spray medicine in 7 days.Every sub-district is 5 samplings at random, whenever click 2 strains, investigate the whole blades of every strain, and are investigating fruit incidence (the same blade of investigation method) in 7 days for the second time with behind the 4th spray medicine.According to the investigation records of graw mold of tomato state of an illness grade scale, calculate disease index and control efficiency.
Following standard is pressed in state of an illness classification:
1. the killed stage division of leaf portion (is unit with the blade):
0 grade, no scab;
1 grade, single blade has 3 of scabs;
3 grades, single blade has 4 ~ 6 of scabs;
5 grades, single blade has 7 ~ 10 of scabs;
7 grades, single blade has 11 ~ 20 of scabs, and part is intensive in flakes;
9 grades, single blade has the intensive blade face that accounts for of scab more than 1/4.
2. the killed stage division of fruit portion (is unit with the single fruit):
0 grade, no scab;
1 grade, residual petal morbidity or column cap morbidity;
3 grades, sepal rots or the column cap morbidity spreads to areola portion;
5 grades, there is the spot of infiltration in areola portion but does not have mould layer;
7 grades, there is mould layer in areola portion but does not expand to other positions;
9 grades, the mould layer of areola portion expands to other positions.
The disease index computing formula:
The control efficiency computing formula:
In the formula: CK
0, CK
1Be the forward and backward disease index of blank district dispenser, PT
0, PT
1Be the forward and backward disease index of treatment region dispenser.
2, result and analysis
2.1 the propamidine complex preparation is to graw mold of tomato field medicine effect test result (blade)
The propamidine complex preparation is seen table 2: to graw mold of tomato field medicine effect test result (blade):
Table 2: the propamidine complex preparation is to graw mold of tomato field medicine effect test result (blade)
Annotate: above test data is 4 times a repetition mean value, uses Duncan ' s duncan's new multiple range method to analyze 5% significance level difference.
2.2 the propamidine complex preparation is to graw mold of tomato field medicine effect test result (fruit)
The propamidine complex preparation is seen table 3 to graw mold of tomato field medicine effect test result (fruit):
Table 3: the propamidine complex preparation is to graw mold of tomato field medicine effect test result (fruit)
Annotate: above test data is 4 times a repetition mean value, uses Duncan ' s duncan's new multiple range method to analyze 5% significance level difference.
Find out that by table 2 and table 3 four kinds of complex preparations have shown graw mold of tomato blade and the higher field efficacy of fruit.2.5%, the propamidine chitosan oligosaccharide complex preparation of 4.5% and 10% 3 proportioning has shown with contrast medicament 40% graw mold of tomato and fruit and has executed the suitable preventive effect of good happy SC.SL200 times of liquid of 4.5% propamidine chitosan oligosaccharide is under conventional spray, and in to the blade disease control, preventive effect is respectively 52.44% and 58.46% after the second time and the 4th dispenser, is superior to contrasting 47.90% and 43.41% of medicament; And the significance difference opposite sex shows, with contrast medicament there were significant differences property.
And in the tamato fruit disease control, 2.5% propamidine chitosan oligosaccharide SL is under 50 times of liquid, and preventive effect is 44.05% and 57.86% after the second time and the 4th dispenser, is superior to contrasting 38.20% and 54.87% of medicament.Other compound drug has also shown higher control efficiency.Explain that both have higher preventive effect at composite grey mold disease to control tomato leaf and fruit.
3: four kinds of propamidine chitosan oligosaccharides of test example complex preparation control cucumber downy mildew field control effectiveness test
1 materials and methods
1.1 supply the reagent agent
2.5% propamidine chitosan oligosaccharide SL, 4.5% propamidine chitosan oligosaccharide SL, 4.5% propamidine chitosan oligosaccharide EC, 10% propamidine chitosan oligosaccharide SL, contrast medicament: 250g/L Fluoxastrobin SC
1.2 supply to study thing
Assorted No. 4 of cucumber Tianjin, the green house plantation.
1.3 controlling object
Cucumber downy mildew (Pseudoperonspora cubensis))
1.4 sub-district design
If 10 processing repeat for 4 times, totally 40 sub-districts, district's group is arranged at random, sub-district area: 6.2m * 1.0m=6.2m
2
1.5 dosage
After confirming every sub-district water consumption, adopt double dilution method to carry out the soup configuration.
Every sub-district water consumption: 1.3L
1.6 investigation method
4 investigation are got in every sub-district, look into two strains at every, and whole blades are investigated in every strain
Stage division (is unit with the blade):
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-l0% of whole leaf area;
5 grades: lesion area accounts for 11%-25% of whole leaf area;
7 grades: lesion area accounts for 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
The disease index computing formula:
The control efficiency computing formula:
In the formula: CK
0, CK
1Be the forward and backward disease index of blank district dispenser, PT
0, PT
1Be the forward and backward disease index of treatment region dispenser.
2 result of the tests
The propamidine complex preparation is seen table 4 to cucumber downy mildew field medicine effect test result (blade):
Table 4: the propamidine complex preparation is to cucumber downy mildew field medicine effect test result (blade)
Can find out that by table 4 four kinds of complex preparations have all shown higher control efficiency to cucumber downy mildew.Preventive effect is all up to more than 50% after the dispenser second time for the compounded combination of 4.5% and 10% content, and effect is also higher after the 4th dispenser, explains that this complex preparation lasting period is longer.Execute good happy SC and review contrast medicament 40%, not good to disease-controlling effect, significance difference opposite sex analytical table is bright, propamidine chitosan oligosaccharide complex preparation and contrast medicament there were significant differences property.
4: four kinds of propamidine chitosan oligosaccharides of test example complex preparation is prevented and treated the sigatoka test of pesticide effectiveness
1, test method
Each handles repetition 4 times, and district's group randomized arrangement prepares soup by concentration, and soup evenly, is thoughtfully sprayed application to the banana tow sides, investigation morbidity radix before the dispenser, and incidence of 7d and 15d investigation calculates disease index and control efficiency after the dispenser.Investigation method is every sub-district random inspection 5 strains, and every all blades of strain banana investigation write down total number of sheets, the sick number of sheets and sick level.According to survey data, calculate control efficiency by (1).
2, sick leaf classification:
0 grade: anosis;
1 grade: scab accounts for leaf area below 1/6;
2 grades: scab accounts for leaf area 1/6-1/3;
3 grades: scab accounts for leaf area 1/3-1/2;
4 grades: scab accounts for leaf area more than 1/2.
3, result of the test
Table 5: four kinds of propamidine chitosan oligosaccharide complex preparations are prevented and treated the sigatoka result
Can be known that by table 5 propamidine chitosan oligosaccharide complex preparation has shown excellent control efficiency in preventing and treating the sigatoka test, 2.5% propamidine chitosan oligosaccharide SL is under 100 times, and 7d and 15d preventive effect are 74.47% and 70.65% after the dispenser; 10% propamidine chitosan oligosaccharide SL is under 400 times, and 7d and 15d preventive effect are respectively 75.42% and 74.88% after the dispenser, all shows to be superior to contrasting the control efficiency that medicament 30% is liked seedling EC.
5: four kinds of propamidine chitosan oligosaccharides of test example complex preparation is prevented and treated the rice sheath blight disease test of pesticide effectiveness
1, testing program
Each handles repetition 4 times, and district's group randomized arrangement prepares soup by concentration, and soup evenly, is thoughtfully sprayed application to the banana tow sides, investigation morbidity radix before the dispenser, incidence of 30d and 50d investigation after the dispenser.Inspection method and computational methods are the same.
2, rice sheath blight disease grade scale:
0 grade: anosis;
1 grade: a small amount of fragmentary morbidity;
3 grades: scab rises to down 3 leaves;
5 grades: scab rises to down 2 leaves;
7 grades: scab rises to down 1 leaf;
9 grades: scab rises to sword-like leave or complete stool is withered.
3, result of the test
Table 6: four kinds of propamidine chitosan oligosaccharide complex preparations are prevented and treated the rice sheath blight disease result
Can find out that by table 6 propamidine chitosan oligosaccharide complex preparation has certain preventive effect to rice sheath blight disease, but effect is a little less than the control efficiency of contrast medicament 5% jinggangmycin A S.4.5% propamidine chitosan oligosaccharide SL is better to the rice sheath blight disease controlling effect, and under 100 times of liquid, 30d and 50d control efficiency are respectively 51.41% and 54.93% after the dispenser, and to carry out significance difference opposite sex analytical table bright with the contrast medicament, and no significance difference is different in nature on 5% level.2.5% and 10% propamidine chitosan oligosaccharide complex preparation has also shown preventive effect preferably.
6: four kinds of propamidine chitosan oligosaccharides of the test example complex preparation control rice blast test of pesticide effectiveness
1, experimental program
Experimental scheme and method are the same.
2, grade scale
2.1 (1) rice blast (panicle blast) grade scale
0 grade: anosis
1 grade: every fringe loss is (morbidity of indivedual branch stalk) below 5%;
3 grades: (branch stalk morbidity about 1/3) below every fringe loss 6%-20%;
5 grades: (ear stem or main shaft morbidity, grain half is flat) below every fringe loss 21-50%;
7 grades: (morbidity of fringe neck, big portion shrivelled kernel) below every fringe loss 51-70%;
9 grades: below every fringe loss 71-100% (morbidity of fringe neck causes dead ears).
2.2 (2) rice blast (leaf pest) classification:
0 grade: anosis.
1: the brown point of syringe needle size.
2: brown point is bigger, and band is circular.
3: be with circular stigma slightly to extend, form the grey scab of edge brown, diameter 1-2 millimeter.
4: typical spindle scab is arranged, and the area of causing harm is less than 2% of leaf area.
5: typical spindle scab is arranged, and hazard area accounts for the 2-10% of leaf area.
6: typical spindle scab is arranged, and hazard area accounts for the 11-25% of leaf area.
7: typical spindle scab is arranged, and hazard area accounts for the 26-50% of leaf area.
8: typical spindle scab is arranged, and hazard area accounts for the 51-75% of leaf area.
9: typical spindle scab is arranged, and hazard area is greater than 75% of leaf area.
3, result of the test
Table 7: four kinds of propamidine chitosan oligosaccharide complex preparation control paddy rice panicle blast results
Table 8: four kinds of propamidine chitosan oligosaccharide complex preparations are prevented and treated the leaf blast in rice result
Can be drawn by data in table 7 and the table 8, propamidine chitosan oligosaccharide complex preparation has certain control efficiency to paddy rice panicle blast and leaf seasonal febrile diseases.4.5% propamidine chitosan oligosaccharide missible oil and soluble liquid are comparatively outstanding to the panicle blast control efficiency, and both are under 100 times of liquid, and 30 days preventive effects are 58.32% and 56.22% after the dispenser; 4.5% propamidine chitosan oligosaccharide SL is in preventing and treating the sick field trial of leaf blast in rice, and preventive effect is respectively 59.41% and 56.93% after 30 days and 50 days.Contrast medicament 5% jinggangmycin A S effect is better, slightly is superior to above medicament.
Other propamidine chitosan oligosaccharide complex preparation also has certain control efficiency to paddy rice panicle blast and leaf seasonal febrile diseases, and for example, 10% propamidine chitosan oligosaccharide SL is under 400 times of dilutions of two types of diseases, and 30 days and 50 days preventive effects all reach more than 50% after the dispenser, have shown good lasting effect.
7: four kinds of propamidine chitosan oligosaccharides of the test example complex preparation control rice green smut test of pesticide effectiveness
1 testing program
Testing program is the same.
2 rice green smut grade scales:
0 grade, not morbidity;
1 grade, 1 rice song;
2 grades, 2 rice songs;
3 grades, 3-5 rice is bent;
4 grades, 6-9 rice is bent;
5 grades: 10 above rice are bent.
3 result of the tests
Four kinds of propamidine chitosan oligosaccharides of table 9 complex preparation control rice green smut result
Can find out that by table 9 four kinds of propamidine chitosan oligosaccharide complex preparations obviously are superior to contrasting medicament 5% jinggangmycin A S to the rice green smut preventive effect.Under SL50 times of liquid of 2.5% propamidine chitosan oligosaccharide and 100 times, after the dispenser 30 days and 50 days preventive effects be 47.47% and 51.09%, 4.5% propamidine chitosan oligosaccharide SL and missible oil under 100 and 200 times, control efficiency also obviously is superior to contrasting medicament.10% propamidine chitosan oligosaccharide is combined under 200 times best to disease-controlling effect, and 30 days and 50 days preventive effects are respectively 51.42% and 50.88%, have also shown the lasting period preferably.
8: four kinds of propamidine chitosan oligosaccharides of test example complex preparation is prevented and treated the cladosporium leaf and fruit mould of tomato test of pesticide effectiveness
1, test method
Arrange immediately the experimental plot, and every processing repetition 4 times was executed medicine one time in 7 days, dispenser twice continuously, 7d check result after the dispenser for the second time.
2, cladosporium leaf and fruit mould of tomato grade scale
0 grade: anosis;
1 grade: scab accounts for whole leaf area below 1/4;
2 grades: scab accounts for whole leaf area below 1/2;
3 grades: scab accounts for whole leaf area below 3/4;
4 grades: scab accounts for whole leaf area more than 3/4;
3, result of the test
Table 10: four kinds of propamidine chitosan oligosaccharide complex preparations are prevented and treated the cladosporium leaf and fruit mould of tomato result
Data show in the table 10, propamidine chitosan oligosaccharide not only can effectively be prevented and treated graw mold of tomato, and cladosporium leaf and fruit mould of tomato is also had good control efficiency.2.5% propamidine chitosan oligosaccharide SL and 4.5% propamidine chitosan oligosaccharide EC and 10% propamidine chitosan oligosaccharide SL are under 50 times, 100 times, 100 times concentration; 15 days control efficiency do not have the significance difference opposite sex all above 60% with preventive effect under the contrast medicament 50% Sukeling WP500 times concentration after the dispenser.Explain that propamidine chitosan oligosaccharide control spectrum is comparatively extensive, multiple diseases is all had certain control efficiency, and prescription is rationally, environmental protection, the noresidue risk is worthy of further application.
4, to other biotic influence
4.1 direct influence to crop
Duration of test does not find that test medicine is to supplying to study deposits yields poisoning phenomenon.
4.2 other biological is influenced
4.2.1 influence to other damage by disease and insect
Do not find that test medicine produces obviously influence to other damage by disease and insect.
4.2.2 influence to beneficial organism
Do not find that test medicine produces obviously influence to beneficial organism.
Though, the present invention has been done detailed description in the preceding text with general explanation and specific embodiments, on basis of the present invention, technical scheme of the present invention to be revised or improved, this will be apparent to those skilled in the art.Therefore, these modifications or the improvement on the basis of not departing from spirit of the present invention, made all belong to the scope that requirement of the present invention is protected.
Claims (5)
1. a bactericidal composition that contains propamidine and chitosan oligosaccharide is characterized in that, the active ingredient of said composition is propamidine and chitosan oligosaccharide, and the mass ratio of said propamidine and chitosan oligosaccharide is 4:1 ~ 8:1, and the preparation form is missible oil, microemulsion, soluble liquid or aqua; Wherein:
Missible oil is formulated according to percentage by weight by following component: propamidine: 0.01%～10%, and chitosan oligosaccharide: 0.01%～5%, emulsifier: 5%～20%, synergist: 1%～10%, solvent is mended to 100%;
Microemulsion is formulated according to percentage by weight by following component; Propamidine: 0.01%～10%, chitosan oligosaccharide: 0.01～5%, emulsifier: 10%～40%, synergist: 1%～10%; Cosolvent: 5～40%; Antifoaming agent: 0.02%, water is mended to 100%;
Soluble liquid is formulated according to percentage by weight by following component: propamidine: 0.01%～10%, and chitosan oligosaccharide: 0.01～5%, emulsifier: 10%～40%, synergist: 1%～10%, solvent is mended to 100%.
Aqua is formulated according to percentage by weight by following component: propamidine: 0.01%～10%, and chitosan oligosaccharide: 0.01%～5%, emulsifier: 10%～40%, synergist: 1%～10%, cosolvent: 1%～40%; Antifoaming agent: 0.02%, water is mended to 100%.
2. the bactericidal composition that contains propamidine and chitosan oligosaccharide as claimed in claim 1 is characterized in that, emulsifier, solvent and synergist used in the said missible oil are respectively:
Emulsifier: one or more mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Solvent: the mixture of one or more in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, the N-Methyl pyrrolidone;
Synergist: the mixture of one or more among oil-soluble azone, AEO, the fast T.
3. the bactericidal composition that contains propamidine and chitosan oligosaccharide as claimed in claim 1 is characterized in that, emulsifier, synergist, cosolvent and antifoaming agent used in the said microemulsion are respectively:
Emulsifier: one or more mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Synergist: the mixture of one or more among oil-soluble azone, AEO, the fast T;
Cosolvent: the mixture of one or more in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, N-Methyl pyrrolidone, the pyroligneous liquor,
Antifoaming agent is selected silicone defoaming agent.
4. the bactericidal composition that contains propamidine and chitosan oligosaccharide as claimed in claim 1 is characterized in that, used emulsifier, synergist and the solvent of said soluble liquid is respectively:
Emulsifier: one or more mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Synergist: the mixture of one or more among oil-soluble azone, AEO, the fast T;
Solvent: the mixture of one or more in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, the N-Methyl pyrrolidone, in the pyroligneous liquor, acetate, water.
5. the bactericidal composition that contains propamidine and chitosan oligosaccharide as claimed in claim 1 is characterized in that, used emulsifier, synergist, cosolvent and the antifoaming agent of said aqua is respectively:
Emulsifier: one or more mixture among agricultural newborn 500#, 600#, 1601#, 1602#, 33#, OP-10, NP-10, OP-15, the 700# etc.;
Cosolvent: the mixture of one or more in dimethyl sulfoxide (DMSO), butyl acetate, citric acid, ethanol, cyclohexanone, acetone, the N-Methyl pyrrolidone, in the pyroligneous liquor, acetate;
Synergist: the mixture of one or more among oil-soluble azone, AEO, the fast T; Solvent is a water.
Antifoaming agent is selected silicone defoaming agent.
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| CN2012102081517A CN102726394A (en) | 2012-06-22 | 2012-06-22 | Sterilization composition containing propamidine and chitosan oligosaccharides |
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| CN2012102081517A CN102726394A (en) | 2012-06-22 | 2012-06-22 | Sterilization composition containing propamidine and chitosan oligosaccharides |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472484A (en) * | 2014-11-27 | 2015-04-01 | 广东中迅农科股份有限公司 | Fungicide composition containing eugenol and propamidine |
| CN105994384A (en) * | 2016-05-21 | 2016-10-12 | 张素香 | Sterilizing composition |
| CN107484781A (en) * | 2017-08-22 | 2017-12-19 | 惠州市锦恒工业模具设计合伙企业(普通合伙) | A kind of bactericidal composition for being used to prevent and treat watermelon anthrax |
| CN110250178A (en) * | 2019-07-02 | 2019-09-20 | 山东亿嘉农化有限公司 | A kind of composite bactericide and preparation method thereof containing bronopol and amino-oligosaccharide |
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2012
- 2012-06-22 CN CN2012102081517A patent/CN102726394A/en active Pending
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| 胡志鹏: "壳寡糖的研究进展", 《中国生化药物杂志》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472484A (en) * | 2014-11-27 | 2015-04-01 | 广东中迅农科股份有限公司 | Fungicide composition containing eugenol and propamidine |
| CN104472484B (en) * | 2014-11-27 | 2017-07-28 | 广东中迅农科股份有限公司 | A kind of bactericidal composition containing eugenol and propamidine |
| CN105994384A (en) * | 2016-05-21 | 2016-10-12 | 张素香 | Sterilizing composition |
| CN107484781A (en) * | 2017-08-22 | 2017-12-19 | 惠州市锦恒工业模具设计合伙企业(普通合伙) | A kind of bactericidal composition for being used to prevent and treat watermelon anthrax |
| CN110250178A (en) * | 2019-07-02 | 2019-09-20 | 山东亿嘉农化有限公司 | A kind of composite bactericide and preparation method thereof containing bronopol and amino-oligosaccharide |
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