CN103975922B - A kind of anti-plant virus composition and application thereof - Google Patents
A kind of anti-plant virus composition and application thereof Download PDFInfo
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- CN103975922B CN103975922B CN201410166856.6A CN201410166856A CN103975922B CN 103975922 B CN103975922 B CN 103975922B CN 201410166856 A CN201410166856 A CN 201410166856A CN 103975922 B CN103975922 B CN 103975922B
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- oligosaccharide
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- triacontanol
- plant virus
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- 239000000203 mixture Substances 0.000 title claims abstract description 36
- 241000700605 Viruses Species 0.000 title claims abstract description 23
- REZQBEBOWJAQKS-UHFFFAOYSA-N triacontan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO REZQBEBOWJAQKS-UHFFFAOYSA-N 0.000 claims abstract description 82
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 39
- 241000209094 Oryza Species 0.000 claims abstract description 17
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 17
- 235000009566 rice Nutrition 0.000 claims abstract description 17
- 201000010099 disease Diseases 0.000 claims abstract description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 16
- 239000007900 aqueous suspension Substances 0.000 claims abstract description 9
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 8
- 238000009736 wetting Methods 0.000 claims abstract description 8
- 239000004615 ingredient Substances 0.000 claims abstract description 4
- 230000002265 prevention Effects 0.000 claims abstract description 4
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 239000005995 Aluminium silicate Substances 0.000 claims description 20
- 235000012211 aluminium silicate Nutrition 0.000 claims description 20
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 6
- 230000003612 virological effect Effects 0.000 abstract description 5
- 238000002791 soaking Methods 0.000 abstract description 4
- 230000000857 drug effect Effects 0.000 abstract description 3
- 230000010496 root system development Effects 0.000 abstract description 2
- 239000007921 spray Substances 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 7
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 208000036142 Viral infection Diseases 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000005648 plant growth regulator Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000256844 Apis mellifera Species 0.000 description 1
- 241000415246 Callisia navicularis Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 108010001682 Dextranase Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000233679 Peronosporaceae Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- MSWZFWKMSRAUBD-QZABAPFNSA-N beta-D-glucosamine Chemical compound N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-QZABAPFNSA-N 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 206010016165 failure to thrive Diseases 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000002015 leaf growth Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Abstract
The invention discloses a kind of anti-plant virus composition and application thereof, belong to anti-plant viral disease pharmaceutical arts.Said composition with amino-oligosaccharide and triacontanol for effective ingredient, wherein, the weight percentage of amino-oligosaccharide is 0.2-2%, the weight percentage of triacontanol is 0.05-2%, make aqueous suspension agent, wetting powder etc., use at rice seed soaking or in seedling stage and rice seedling bed, root system development can be promoted and tiller, increase yield, very remarkable to bakanae disease control efficiency.Compared with prior art, composition of the present invention, with the mixture of amino-oligosaccharide and triacontanol, significantly improves drug effect, can produce good prevention and treatment effect on plant viral diseases compared with the amino-oligosaccharide of low dosage.
Description
Technical field
The present invention relates to anti-plant viral disease pharmaceutical arts, specifically a kind of containing amino-oligosaccharide and the anti-plant virus composition of triacontanol and the application of said composition.
Background technology
Current cosmopolitian plant viral species has reached 909 kinds, China has reported kind more than 100, most economic crops are all because the harm being subject to virus disease makes plant part or whole growth failure, serious obstruction plant growth, affect quality, reduce output, common sympton has withered, mottled and rotten.Viroses of plant kind is many, endangers wide, control difficulty.
Amino-oligosaccharide (chitosan oligosaccharide) refers to the oligosaccharide that D-Glucosamine connects with β-1.4 glycosidic bond, and after obtaining shitosan by chitin degrading, degraded obtains again, or the low toxicity bactericide extracted by fermentable.Amino-oligosaccharide (agriculture level chitosan oligosaccharide) can produce inhibitory action to the growth of some germs, affect fungus spore germination, brings out that hypha form morphs, biochemistry changes in spore.Can excite plant vivo gene, produce and have the chitinase of resistant effect, dextranase, guarantor's element and PR albumen etc., and have cell activation effect, the recovery of the plant that contributes to being injured, short root strong sprout, strengthen the resistance of crop, Promoting plant growth is grown.Amino-oligosacchride cellulose solution, has virus killing, kills bacterium, fungicidal action.Not only to fungi, bacterium, virus, there is extremely strong control and eradicant action, but also there is nutrition, adjustment, removing toxic substances, antibacterial effect.The disease such as virus, bacterium, fungus-caused mosaic disease, little leaf, spot disease, anthracnose, downy mildew, epidemic disease, blight dis-ease, yellow dwarf, rice blast, bacterial wilt, soft rot of control fruit tree, vegetables, underground rhizome, tobacco, traditional Chinese medicine and grain and cotton crop can be widely used in.
Present invention applicant has developed the female medicine of amino-oligosaccharide and aqua product, and popularization is used for preventing and treating the viroses of plant, but when finding that single use Mushroom polysaccharide dosage is low, drug effect is also lower, and when using dosage is excessive, cost is too high.
Triacontanol, English name: triacontanol, CAS accession number: 593-50-0.Triacontanol is a kind of natural Long carbon chain plant growth regulator, chemical name N-triacontanol, structural formula CH3(CH2) 28CH2OH, also known as melissyl alcohol, is the natural biological product of purification from honeybee wax.To person poultry harmless and side effect, environmentally safe.Flourishing agricultural country, spray triacontanol as far back as the 1970s and 1980s in 20th century comprehensively, as the good harvest of agricultural high yield, optimize the plant growth regulator of quality structure, triacontanol can absorb via the stem of plant, leaf, then promote the growth of plant, increase dry matter accumulation, improve cell membrane permeability, increase chlorophyllous content, improve photosynthetic intensity, strengthen amylase, polyoxygenated enzyme, peroxidase activity.Although single use triacontanol can stratification, take root, cauline leaf growth and blooming, make crops precocious, improve ripening rate, strengthen cold-resistant, drought-resistant ability, increase yield, improve product quality, be difficult to prevent and treat the infringement of crop from the viroses of plant.
Summary of the invention
Technical assignment of the present invention is for above-mentioned the deficiencies in the prior art, provides a kind of anti-plant virus composition.
The further technical assignment of the present invention is to provide the application of above-mentioned composition.
Technical assignment of the present invention realizes in the following manner: a kind of anti-plant virus composition, be characterized in amino-oligosaccharide and triacontanol as effective ingredient, wherein, the weight percentage of amino-oligosaccharide is 0.2-2%, and the weight percentage of triacontanol is 0.05-2%.
The weight percentage of amino-oligosaccharide is preferably 1-2%, and the weight percentage of triacontanol is preferably 1-2%.
Above-mentioned composition can be aqueous suspension agent or wetting powder.
When above-mentioned composition is aqueous suspension agent, be made up of the component of following weight percentage:
Amino-oligosaccharide 0.2-2%
Triacontanol 0.05-2%
Lauryl sodium sulfate 3%
NNO8%
Kaolin 2%
Water surplus.
Be preferably:
Amino-oligosaccharide 1-2%
Triacontanol 1-2%
Lauryl sodium sulfate 3%
NNO8%
Kaolin 2%
Water surplus.
Aqueous suspension agent preparation method is as follows: after amino-oligosaccharide, triacontanol, lauryl sodium sulfate, NNO, kaolin being mixed in proportion, be crushed to 300--400 order, fill up water, and namely mixing becomes aqueous suspension agent.
When above-mentioned composition is wetting powder, be made up of the component of following weight percentage:
Amino-oligosaccharide 0.5-2%
Triacontanol 0.05-2%
Lauryl sodium sulfate 3%
White carbon 5-6%
NNO5-10%
Kaolin surplus.
Be preferably:
Amino-oligosaccharide 1.5-2%
Triacontanol 1-2%
Lauryl sodium sulfate 3%
White carbon 6%
NNO8%
Kaolin surplus.
The preparation method of wetting powder is as follows: amino-oligosaccharide, triacontanol, lauryl sodium sulfate, white carbon, NNO, kaolin are mixed in proportion, wherein lauryl sodium sulfate is as dispersant, white carbon is as wetting agent, NNO is as diffusant, kaolin is auxiliary material, and amino-oligosaccharide and triacontanol are as effective ingredient.After each component mixing, they are crushed to 300--400 order, obtain present composition wetting powder.
The application of above-mentioned anti-plant virus composition in the water prevention rice bakanae disease.
The application method of above-mentioned application: anti-plant virus composition dilute with water 200-300 is doubly soaked seed, or by anti-plant virus composition dilute with water 500-1000 doubly, within 8-12 days, be sprayed on crop at rice seedling or after transplanting.
Preferred seed soaking time is 24 hours.
Preferred spray method is: spray twice on crop, interval 7-10 days between twice.
Anti-plant virus composition of the present invention and application thereof compared with prior art have following outstanding beneficial effect:
(1) mixture of amino-oligosaccharide and triacontanol, significantly improves drug effect, can produce good prevention and treatment effect on plant viral diseases, have application value well compared with the amino-oligosaccharide of low dosage;
(2) aqueous suspension agent or wetting powder, is convenient to spray, easy to use;
(3) while preventing and treating the infringement of crop from the viroses of plant, root system development can be promoted and tiller, increase yield.
Embodiment
With specific embodiment, test example, anti-plant virus composition of the present invention and application thereof are described in detail below.
If no special instructions, the content of following each composition used is weight percentage content.
Example 1
Component and content: amino-oligosaccharide 0.2%, triacontanol 0.25%, lauryl sodium sulfate 3%, NNO8%, kaolin 2%, water is supplied.
Method for making: be crushed to 300--400 order after amino-oligosaccharide, triacontanol, lauryl sodium sulfate, NNO, kaolin being mixed in proportion, fill up water, namely dissolving, mixing become aqueous suspension agent.
Example 2
Component and content: amino-oligosaccharide 0.25%, triacontanol 0.5%, lauryl sodium sulfate 3%, NNO8%, kaolin 2%, water is supplied.
Preparation method is with example 1.
Example 3
Component and content: amino-oligosaccharide 0.5%, triacontanol 2%, lauryl sodium sulfate 3%, NNO8%, kaolin 2%, water is supplied.
Preparation method is with example 1.
Example 4
Component and content: amino-oligosaccharide 2%, triacontanol 2%, lauryl sodium sulfate 3%, NNO8%, kaolin 2%, water is supplied.
Preparation method is with example 1.
Example 5
Component and content: amino-oligosaccharide 1%, triacontanol 1%, lauryl sodium sulfate 3%, white carbon 6%, NNO5%, kaolin surplus.
Method for making: amino-oligosaccharide, triacontanol, lauryl sodium sulfate, white carbon, NNO, kaolin are mixed in proportion, is crushed to 300--400 order, obtains the present composition.
Example 6
Component and content: amino-oligosaccharide 2%, triacontanol 0.1%, lauryl sodium sulfate 3%, white carbon 6%, NNO5%, kaolin surplus.
Preparation method is with example 5.
Example 7
Component and content: amino-oligosaccharide 2%, triacontanol 0.5%, lauryl sodium sulfate 3%, white carbon 6%, NNO5%, kaolin surplus.
Preparation method is with example 5
Comparative example 1
Component is: amino-oligosaccharide 0.2%, lauryl sodium sulfate 3%, ethanol 10%, and water is supplied (solvent).
Comparative example 2
Component is: triacontanol 0.25%, lauryl sodium sulfate 3%, ethanol 10%, and water is supplied (solvent).
The using method of the composition that above-mentioned example and comparative example obtain is as follows: by above-mentioned obtained composition difference dilute with water 250 times and 500 times, seed soaking is carried out 24 hours to paddy rice, or dilute with water 500 times and 1000 times, spray at rice seedling, every 8 days, after spray again once.
Table 1 dilute with water 250 times and 500 times carry out seed soaking 24 hours to paddy rice, shoot transplanting equipment inspection its control efficiency to bakanae disease of rice of contrast (totally 17 test units, every unit 1.5m × 10m) in first 10 days:
Table 2 dilute with water 500 times and 1000 times, spray at rice seedling, every 8 days, after spray again once.Control efficiency to rice seedling, rice seedling bed bakanae disease:
As can be seen from the above table, the amino-oligosaccharide control efficiency mixed with triacontanol is good, as can be seen from contrast with comparative example 1 and 2 of example 1, composition of the present invention is than being used alone the amino-oligosaccharide of identical dose or triacontanol to rice seedling and rice seedling bed, and the control efficiency of bakanae disease is remarkable.
Claims (9)
1. an anti-plant virus composition, is characterized in that: with amino-oligosaccharide and triacontanol for effective ingredient, wherein, the weight percentage of amino-oligosaccharide is 0.2-2%, and the weight percentage of triacontanol is 0.05-2%.
2. anti-plant virus composition according to claim 1, is characterized in that: described composition is aqueous suspension agent, is made up of the component of following weight percentage:
Amino-oligosaccharide 0.2-2%
Triacontanol 0.05-2%
Lauryl sodium sulfate 3%
NNO8%
Kaolin 2%
Water surplus.
3. anti-plant virus composition according to claim 2, is characterized in that: described aqueous suspension agent is made up of the component of following weight percentage:
Amino-oligosaccharide 1-2%
Triacontanol 1-2%
Lauryl sodium sulfate 3%
NNO8%
Kaolin 2%
Water surplus.
4. anti-plant virus composition according to claim 1, is characterized in that: described composition is wetting powder, is made up of the component of following weight percentage:
Amino-oligosaccharide 0.5-2%
Triacontanol 0.05-2%
Lauryl sodium sulfate 3%
White carbon 5-6%
NNO5-10%
Kaolin surplus.
5. anti-plant virus composition according to claim 4, is characterized in that: described wetting powder, is made up of the component of following weight percentage:
Amino-oligosaccharide 1.5-2%
Triacontanol 1-2%
Lauryl sodium sulfate 3%
White carbon 6%
NNO8%
Kaolin surplus.
6. the application of anti-plant virus composition in the water prevention rice bakanae disease described in claim 1,2,3,4 or 5.
7. application according to claim 6, it is characterized in that: anti-plant virus composition dilute with water 200-300 is doubly soaked seed, or by anti-plant virus composition dilute with water 500-1000 doubly, within 8-12 days, be sprayed on crop at rice seedling or after transplanting.
8. application according to claim 7, is characterized in that: soak seed 24 hours.
9. application according to claim 7, is characterized in that: within 5-15 days, to be sprayed on crop twice at rice seedling or after transplanting, interval 7-10 days between twice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410166856.6A CN103975922B (en) | 2014-04-24 | 2014-04-24 | A kind of anti-plant virus composition and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410166856.6A CN103975922B (en) | 2014-04-24 | 2014-04-24 | A kind of anti-plant virus composition and application thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN103975922A CN103975922A (en) | 2014-08-13 |
| CN103975922B true CN103975922B (en) | 2016-01-13 |
Family
ID=51268299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410166856.6A Active CN103975922B (en) | 2014-04-24 | 2014-04-24 | A kind of anti-plant virus composition and application thereof |
Country Status (1)
| Country | Link |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112913842A (en) * | 2021-02-01 | 2021-06-08 | 渭南东旺农华生物科技有限公司 | Functional agricultural composition containing triacontanol and glycan |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4420329A (en) * | 1981-06-15 | 1983-12-13 | The Procter & Gamble Company | Stable colloidal dispersions of triacontanol |
| CN1792164A (en) * | 2006-01-06 | 2006-06-28 | 胡小安 | Plant growth regulator |
| CN101306964A (en) * | 2008-07-09 | 2008-11-19 | 厦门大学 | Winter green-guarding promoting agent for lawn of warming season type and method for preparing same |
-
2014
- 2014-04-24 CN CN201410166856.6A patent/CN103975922B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4420329A (en) * | 1981-06-15 | 1983-12-13 | The Procter & Gamble Company | Stable colloidal dispersions of triacontanol |
| CN1792164A (en) * | 2006-01-06 | 2006-06-28 | 胡小安 | Plant growth regulator |
| CN101306964A (en) * | 2008-07-09 | 2008-11-19 | 厦门大学 | Winter green-guarding promoting agent for lawn of warming season type and method for preparing same |
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| CN103975922A (en) | 2014-08-13 |
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