A kind of anti-plant virus composition and application thereof
Technical field
The present invention relates to anti-plant viral disease pharmaceutical arts, specifically a kind of containing mushroom proteoglycan and the anti-plant virus composition of triacontanol and the application of said composition.
Background technology
Current cosmopolitian plant viral species has reached 909 kinds, China has reported kind more than 100, most economic crops are all because the harm being subject to virus disease makes plant part or whole growth failure, serious obstruction plant growth, affect quality, reduce output, common sympton has withered, mottled and rotten.Viroses of plant kind is many, endangers wide, control difficulty.
Mushroom proteoglycan is the effective active components extracted from high-quality mushroom class entity, is a kind of host immunity reinforcing agent, has the function that antiviral, antitumor, function and stimulation interferon are formed.The composition that mushroom proteoglycan improves immunity is the polysaccharide of molecular weight more than 10,000, and mushroom proteoglycan has been used for the control of the viroses of plant by current China.
Present invention applicant has developed the female medicine of mushroom proteoglycan and aqua product, and popularization is used for preventing and treating the viroses of plant, but when finding that single use Mushroom polysaccharide dosage is low, drug effect is also lower, and when using dosage is excessive, cost is too high.
Triacontanol, English name: triacontanol, CAS accession number: 593-50-0.Triacontanol is a kind of natural Long carbon chain plant growth regulator, chemical name N-triacontanol, structural formula CH3(CH2) 28CH2OH, also known as melissyl alcohol, is the natural biological product of purification from honeybee wax.To person poultry harmless and side effect, environmentally safe.Flourishing agricultural country, spray triacontanol as far back as the 1970s and 1980s in 20th century comprehensively, as the good harvest of agricultural high yield, optimize the plant growth regulator of quality structure, triacontanol can absorb via the stem of plant, leaf, then promote the growth of plant, increase dry matter accumulation, improve cell membrane permeability, increase chlorophyllous content, improve photosynthetic intensity, strengthen amylase, polyoxygenated enzyme, peroxidase activity.Although single use triacontanol can stratification, take root, cauline leaf growth and blooming, make crops precocious, improve ripening rate, strengthen cold-resistant, drought-resistant ability, increase yield, improve product quality, be difficult to prevent and treat the infringement of crop from the viroses of plant.
Summary of the invention
Technical assignment of the present invention is for above-mentioned the deficiencies in the prior art, provides a kind of anti-plant virus composition.
The further technical assignment of the present invention is to provide the application of above-mentioned composition.
Technical assignment of the present invention realizes in the following manner: a kind of anti-plant virus composition, be characterized in mushroom proteoglycan and triacontanol as effective ingredient, wherein, the weight percentage of mushroom proteoglycan is 0.5-2%, and the weight percentage of triacontanol is 0.05-2%.
The weight percentage of mushroom proteoglycan is preferably 1-2%, and the weight percentage of triacontanol is preferably 1-2%.
Above-mentioned composition can be aqueous suspension agent or wetting powder (soluble powder).
When above-mentioned composition is aqueous suspension agent, be made up of the component of following weight percentage:
Mushroom proteoglycan 0.5-2%
Triacontanol 0.05-2%
Lauryl sodium sulfate 3%
NNO8%
Water surplus.
Be preferably:
Mushroom proteoglycan 1-2%
Triacontanol 1-2%
Lauryl sodium sulfate 3%
NNO8%
Water surplus.
Aqueous suspension agent preparation method is as follows: after mushroom proteoglycan, triacontanol, lauryl sodium sulfate, NNO, kaolin being mixed in proportion, be crushed to 300--400 order, fill up water, and namely dissolving, mixing become aqueous suspension agent.
When above-mentioned composition is wetting powder (soluble powder), be made up of the component of following weight percentage:
Mushroom proteoglycan 0.5-2%
Triacontanol 0.05-2%
Lauryl sodium sulfate 3%
White carbon 5-6%
NNO5-10%
Kaolin surplus.
Be preferably:
Mushroom proteoglycan 1-2%
Triacontanol 1-2%
Lauryl sodium sulfate 3%
White carbon 6%
NNO8%
Kaolin surplus.
The preparation method of wetting powder is as follows: mushroom proteoglycan, triacontanol, lauryl sodium sulfate, white carbon, NNO, kaolin are mixed in proportion, wherein lauryl sodium sulfate is as dispersant, white carbon is as wetting agent, NNO is as diffusant, kaolin is auxiliary material, and mushroom proteoglycan and triacontanol are as effective ingredient.After each component mixing, they are crushed to 300--400 order, obtain present composition wetting powder.
The application of above-mentioned anti-plant virus composition in control tomato virus disease or tobacco virus.
Embody rule method: by described composition dilute with water 500-1000 doubly, be sprayed on crop at virus disease initial stage of origination.(time that namely seedling strain is transplanted latter about 10 days mostly is virus disease initial phase.)
Preferred spray method is: spray twice on crop, interval 7-10 days between twice.
Anti-plant virus composition of the present invention and application thereof compared with prior art have following outstanding beneficial effect:
(1) mixture of mushroom proteoglycan and triacontanol, significantly improves drug effect, can produce good prevention and treatment effect on plant viral diseases, have application value well compared with the mushroom proteoglycan of low dosage;
(2) aqueous suspension agent or wetting powder, is convenient to spray, easy to use;
(3) while the infringement of control crop from the viroses of plant, stratification, take root, cauline leaf growth and blooming, improve ripening rate, improve product quality.
Embodiment
With specific embodiment, test example, anti-plant virus composition of the present invention and application thereof are described in detail below.
If no special instructions, the content of following each composition used is weight percentage content.
Example 1
Component and content:
Mushroom proteoglycan 0.5%, triacontanol 1%, lauryl sodium sulfate 3%, NNO8%, water is supplied.
Method for making:
By mushroom proteoglycan, triacontanol, they, after NNO mixes in proportion, are crushed to 300 by lauryl sodium sulfate---and 400 orders, fill up water, namely dissolving, mixing become aqueous suspension agent.
Example 2
Component and content:
Mushroom proteoglycan 0.5%, triacontanol 0.5%, lauryl sodium sulfate 3%, NNO8%, water is supplied.
Preparation method is with example 1.
Example 3
Component and content: mushroom proteoglycan 1%, triacontanol 2%, lauryl sodium sulfate 3%, NNO8%, water is supplied.
Preparation method is with example 1.
Example 4
Component and content: mushroom proteoglycan 2%, triacontanol 2%, lauryl sodium sulfate 3%, NNO8%, water is supplied.
Preparation method is with example 1.
Example 5
Component and content: mushroom proteoglycan 1%, triacontanol 1%, lauryl sodium sulfate 3%, white carbon 6%, NNO5%, kaolin surplus.
Method for making:
Mushroom proteoglycan, triacontanol, lauryl sodium sulfate, white carbon, NNO, kaolin being mixed in proportion, are crushed to 300---400 orders, obtain present composition wetting powder.
Example 6
Component and content: mushroom proteoglycan 2%, triacontanol 1%, lauryl sodium sulfate 3%, white carbon 6%, NNO5%, kaolin surplus.
Preparation method is with example 5
Example 7
Component and content: mushroom proteoglycan 2%, triacontanol 0.5%, lauryl sodium sulfate 3%, white carbon 6%, NNO5%, kaolin surplus.
Preparation method is with example 5
Comparative example 1
Component is: mushroom proteoglycan 0.5%, lauryl sodium sulfate 3%, ethanol 10%, and water is supplied (solvent).
Comparative example 2
Component is: triacontanol 1%, lauryl sodium sulfate 3%, ethanol 10%, and water is supplied (solvent).
The using method of the composition that above-mentioned example and comparative example obtain is as follows: by above-mentioned obtained composition difference dilute with water 500 times and 1000 times, at tomato virus disease or tobacco virus their early stage, namely seedling strain is transplanted latter 10 days, sprays, every 8 days, after spray again once.
The virus of harm tobacco is mainly tobacco mosaic virus (TMV), is also Tomato mosaic virus (TOMV), and the virus of harm tomato mainly contains Tomato mosaic virus (TOMV), cucumber mosaic virus (CWV), potato virus X (PVY).Be described for tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) control efficiency to the present composition below.
Table 1 pair tobacco virus (TMV) control efficiency
Table 2 pair Cucumber Mosaic Virus (CMV) control efficiency
* data heel with difference letter, to represent in variance analysis significant difference (DMRT method) in F0.05 level.
The present composition is also close with cucumber mosaic virus (CMV) to the control efficiency of potato virus X (PVY).As can be seen from the above table, the mushroom proteoglycan control efficiency mixed with triacontanol is good, as can be seen from contrast with comparative example 1 and 2 of example 1, composition of the present invention is more remarkable than the mushroom proteoglycan or the control efficiency of triacontanol to tomato virus disease being used alone identical dose.