CN102047883B - Plant virus disease-resistant composition containing lentinan and brassinolide and application thereof - Google Patents

Plant virus disease-resistant composition containing lentinan and brassinolide and application thereof Download PDF

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CN102047883B
CN102047883B CN2011100278988A CN201110027898A CN102047883B CN 102047883 B CN102047883 B CN 102047883B CN 2011100278988 A CN2011100278988 A CN 2011100278988A CN 201110027898 A CN201110027898 A CN 201110027898A CN 102047883 B CN102047883 B CN 102047883B
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composition
lentinan
brassin lactones
plant
virus
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CN102047883A (en
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魏来忠
孟凡顺
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Shandong Shengpeng Technology Co ltd
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SHANDONG SHENGPENG PESTICIDE CO Ltd
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Abstract

The invention relates to the field of plant virus disease-resistant agents, in particular to a plant virus disease-resistant composition and application thereof. In the composition, 0.5 to 5 weight percent of lentinan and 0.0002 to 0.02 weight percent of brassinolide are used as active ingredients. The composition is prepared into water aqua by adding ethanol, sodium dodecylsulfate and water and is prepared into soluble powder or wettable powder by adding the sodium dodecylsulfate, white carbon black, sodium methylenedinaphthalene disulphonate (NNO) and kaoline. The plant virus disease-resistant composition is applied to prevention and control of virus diseases of tomato or tobacco. The composition has good prevention and control effect on plant virus diseases and can induce and enhance plant immunity so as to suppress infection of virus and achieve antivirus and production increasing effects; the composition can be prepared into the water aqua, the soluble powder or the wettable powder, has various formulations and is flexible in use; the composition has effects of promoting growth, and preventing and treating the virus diseases, is a plant agent which has high cost performance and has obvious growth-promoting effect; and the composition has the advantages of simple preparation method, readily available raw materials and broad market prospect.

Description

A kind of anti-plant virus composition and application thereof that contains lentinan and brassin lactones
Technical field
The present invention relates to anti-plant viral disease medicament field, particularly a kind of anti-plant virus composition that contains lentinan and brassin lactones also relates to the application of said composition.
Background technology
Mushroom (Lentinus edodes) is the basidiomycetes of Pleurotaceae (Pleara taco), contains multiple effective medicinal ingredient.(Lentinan LNT), is the effective active composition that from the quality xianggu fruit body, extracts to lentinan, is a kind of host immunity reinforcing agent, and that lentinan has is antiviral, antitumor, regulate immunologic function and stimulate functions such as interferon formation.The composition that lentinan improves phyto-immunity is that molecular weight surpasses 10,000 polysaccharide, is main with β-1,3 glucan, contains a spot of wood sugar and mannose, and China is used for medicine with lentinan at present, and Mushroom polysaccharide is used for the control of the viroses of plant.The application number of my unit application is 201010226519.3; Name is called the application for a patent for invention of " a kind of anti-plant virus composition and application thereof that contains lentinan and ribavirin ", discloses a kind of anti-plant virus composition that contains lentinan and ribavirin.The residual of composition is enriched in the human body for a long time, and particularly ribavirin can cause that leukopenia, anaemia, serum transaminase and bilirubin raise.
(brassinolide, BR) have another name called brassinosteroid is a kind of new plant endogenous hormones to brassin lactones, regulates and the growth that promotes plant comprehensively, gives full play to growth vigor.This compound is as a kind of novel steroidal plant hormone, and its main effect improves seed vitality for improving the activity of plant corpus endoenzyme, promotes early development; Strengthen photosynthetic efficiency, improve crop yield, improve crop quality; Improve cold-resistant, drought-enduring, waterlogging, the salt tolerant alkali ability of crop, improve anti-adversity ability.Brassin lactones mainly is to use as plant growth regulating promoter at present.
Summary of the invention
In order to overcome the above problems, the invention provides a kind of composition that contains the anti-plant viral disease of lentinan and brassin lactones.Said composition has improved the effect of lentinan and brassin lactones anti-plant viral disease, and effect is remarkable preventing and treating aspect the viroses of plant.
Another object of the present invention provides the application of this anti-plant virus composition in control tomato virus disease or tobacco virus.
The present invention realizes through following measure:
A kind of anti-plant virus composition that contains lentinan and brassin lactones is an active ingredient with lentinan and brassin lactones, and wherein, the content of lentinan is 0.5-5wt%, and the content of brassin lactones is 0.0002-0.02wt%.
Said composition is an aqua, comprises the component of following percetage by weight:
Lentinan 0.5-5%
Brassin lactones 0.0002-0.02%
Ethanol 10%
Lauryl sodium sulfate 3%
Water surplus.
Said composition is soluble powder or wetting powder, comprises the component of following percetage by weight:
Lentinan 0.5-5%
Brassin lactones 0.0002-0.02%
Lauryl sodium sulfate 3%
White carbon 5-6%
NNO 5-10%
The kaolin surplus.
Described Antiphytoviral composition preferably includes the component of following percetage by weight:
Lentinan 3-4%
Brassin lactones 0.01-0.02%
Ethanol 10%
Lauryl sodium sulfate 3%
Water surplus.
Described Antiphytoviral composition preferably includes the component of following percetage by weight:
Lentinan 3-4%
Brassin lactones 0.01-0.02%
Lauryl sodium sulfate 3%
White carbon 6%
NNO 8%
The kaolin surplus.
The mass ratio of lentinan and brassin lactones is 100-500:1.
The mass ratio of lentinan and brassin lactones is preferably 200-300:1.
The described application of anti-plant virus composition in control tomato virus disease or tobacco virus that contains lentinan and brassin lactones with said composition dilute with water 500-1000 doubly, is sprayed on the crop at the virus disease initial stage of origination.
The interval is 7-10 days said composition sprays twice, twice on crop between.
The preparation method of above-mentioned composition:
Aqua: use the dissolve with ethanol brassin lactones earlier, add the female medicine mixing of entry and lentinan, adding lauryl sodium sulfate dissolving and mixing more promptly becomes aqua.
Soluble powder or wetting powder: lentinan, brassin lactones, lauryl sodium sulfate, white carbon, NNO and kaolin are mixed by proportioning.Wherein, as diffusant, kaolin is as auxiliary material as wetting agent, NNO for white carbon, and lentinan, brassin lactones and lauryl sodium sulfate are effective ingredient.After each component mixes, they are crushed to the 300-400 order, promptly get the present composition.
NNO: dispersant, molecular formula: CH 2(C 10H 6SO 3Na) 2Or C 21H 14O 6S 2Na 2, molecular weight: 472.44, CAS number: 36290-04-7.
The invention has the beneficial effects as follows:
1, finds that through a large amount of experiments the composition that lentinan and brassin lactones make is good to viroses of plant control efficiency, can induce and enhancement of plant immunity, thereby suppress infecting of virus, reach effect antiviral and raising the output;
2, spray in vegetative period, virus disease is had the excellent prevention effect, fall ill period, good result of treatment is arranged in virus disease;
3, can be prepared into aqua, soluble powder or wetting powder, the formulation variation is used flexibly;
4, composition of the present invention has the effect that promotes growth, raising the output, prevention and treatment virus disease concurrently, is that a kind of cost performance is high, increases significant plant medical agent, and the preparation method is simple, and raw material is easy to get, and has vast market prospect.
Embodiment
Through specific embodiment the present invention is set forth in detail below, following explanation only is exemplary, does not limit the invention.If no special instructions, following percentage is percetage by weight.
Instance 1
Component and content: lentinan 1%, brassin lactones 0.001%, ethanol 10%, lauryl sodium sulfate 3%, surplus are water (solvent).
Method for making: use the dissolve with ethanol brassin lactones earlier, add the female medicine mixing of entry and lentinan, adding lauryl sodium sulfate dissolving and mixing more promptly becomes aqua.
Instance 2
Component and content: lentinan 0.5%, brassin lactones 0.0002%, ethanol 10%, lauryl sodium sulfate 3%, surplus are water (solvent).
The preparation method is with embodiment 1.
Instance 3
Component and content: lentinan 3%, brassin lactones 0.01%, ethanol 10%, lauryl sodium sulfate 3%, surplus are water (solvent).
The preparation method is with embodiment 1.
Instance 4
Component and content: lentinan 4%, brassin lactones 0.02%, lauryl sodium sulfate 3%, white carbon 6%, NNO 8%, surplus are kaolin.
Method for making: lentinan, brassin lactones, lauryl sodium sulfate, white carbon, NNO and kaolin are mixed by proportioning, after each component mixes, they are crushed to the 300-400 order, promptly get composition.
Instance 5
Component and content: lentinan 5%, brassin lactones 0.02%, lauryl sodium sulfate 3%, white carbon 6%, NNO 5%, surplus are kaolin.
The preparation method is with embodiment 4.
Instance 6
Component and content: lentinan 1%, brassin lactones 0.0015%, lauryl sodium sulfate 3%, white carbon 5%, NNO 10%, surplus are kaolin.
The preparation method is with embodiment 4.
Instance 7
Component and content: lentinan 2.5%, brassin lactones 0.005%, ethanol 10%, lauryl sodium sulfate 3%, surplus are water (solvent).
The preparation method is with embodiment 1.
Comparative Examples 1
Compare with embodiment 1, component is: lentinan 1%, lauryl sodium sulfate 3%, surplus are water (solvent).
Comparative Examples 2
Application number is 201010226519.3 the disclosed composition of Chinese invention patent application.
The method for using of the composition that the foregoing description and Comparative Examples make is following: with 500 times and 1000 times of the above-mentioned composition difference dilute with waters that makes, spray at tomato virus disease or tobacco virus their early stage, at a distance from spraying once after 7-10 days again.
The measure of merit experiment
The virus of harm tobacco is mainly tobacco mosaic virus (TMV), also is TOMV (TOMV).The virus of harm tomato mainly contains TOMV (TOMV), cucumber mosaic virus (CMV), potato virus X (PVY), and being example with tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) below describes the control efficiency of the present composition.Tobacco virus (TMV) control efficiency is seen table 1, tomato virus disease (CMV) control efficiency is seen table 2.
Table 1 pair tobacco virus (TMV) control efficiency
Figure DEST_PATH_IMAGE001
Table 2 pair tomato virus disease (CMV) control efficiency
Figure 946087DEST_PATH_IMAGE002
* the data heel with different letters, the expression variance analysis is at F 0.05Significant difference on the level.(DMRT method).
The present composition is also close with cucumber mosaic virus (CMV) to the control efficiency of potato virus X (PVY).
Can find out from last table 1 and table 2; Comparative Examples 2 (application number is 201010226519.3 the disclosed composition of Chinese invention patent application) is put down in writing according to patent application specification the control efficiency of tobacco and tomato virus disease has only 78.75% and 79.9% preferably; Use separately the control efficiency of lentinan all to have only 41.25%, and composition of the present invention has only been added brassin lactones, mostly reached more than 80% the control efficiency of virus disease; Embodiment 3 and 4 has reached 90%; Had significantly and obviously improved, the control efficiency that lentinan and brassin lactones are used with is good, and especially the effect of embodiment 3 and embodiment 4 is best.Embodiment 4 adds after the filler auxiliary agent, and composition time of staying on plant is more of a specified duration, and the drug effect performance is more lasting, so effect is also better.
Analyze reason, brassin lactones has the effect that promotes plant growing, can improve via plant immunity, sprays in the virus disease period of disease; Use with lentinan, not only sterilization but also from nursing one's health has inside reached a well synergy; Therefore virus disease is had good control efficiency, more effective than the outside bactericide of independent use, good to viroses of plant control efficiency; Can induce and enhancement of plant immunity, thereby suppress infecting of virus, reach effect antiviral and raising the output.

Claims (11)

1. anti-plant virus composition that contains lentinan and brassin lactones, it is characterized in that: with lentinan and brassin lactones is active ingredient, and wherein, the content of lentinan is 0.5-5wt%, and the content of brassin lactones is 0.0002-0.02wt%,
The mass ratio of said lentinan and brassin lactones is 200-300:1.
2. anti-plant virus composition that contains lentinan and brassin lactones, it is characterized in that: said composition is an aqua, is made up of the component of following percetage by weight:
Lentinan 0.5-5%
Brassin lactones 0.0002-0.02%
Ethanol 10%
Lauryl sodium sulfate 3%
Water surplus.
3. anti-plant virus composition that contains lentinan and brassin lactones, it is characterized in that: said composition is soluble powder or wetting powder, is made up of the component of following percetage by weight:
Lentinan 0.5-5%
Brassin lactones 0.0002-0.02%
Lauryl sodium sulfate 3%
White carbon 5-6%
NNO 5-10%
The kaolin surplus.
4. anti-plant virus composition according to claim 2 is characterized in that being made up of the component of following percetage by weight:
Lentinan 3-4%
Brassin lactones 0.01-0.02%
Ethanol 10%
Lauryl sodium sulfate 3%
Water surplus.
5. anti-plant virus composition according to claim 3 is characterized in that being made up of the component of following percetage by weight:
Lentinan 3-4%
Brassin lactones 0.01-0.02%
Lauryl sodium sulfate 3%
White carbon 6%
NNO 8%
The kaolin surplus.
6. the application of anti-plant virus composition in control tomato virus disease or tobacco virus that contains lentinan and brassin lactones is characterized in that: with said composition dilute with water 500-1000 doubly, be sprayed on the crop at the virus disease initial stage of origination;
Said anti-plant virus composition is an active ingredient with lentinan and brassin lactones, and wherein, the content of lentinan is 0.5-5wt%, and the content of brassin lactones is 0.0002-0.02wt%.
7. application according to claim 6 is characterized in that: said composition is an aqua, is made up of the component of following percetage by weight:
Lentinan 0.5-5%
Brassin lactones 0.0002-0.02%
Ethanol 10%
Lauryl sodium sulfate 3%
Water surplus.
8. application according to claim 6 is characterized in that: said composition is soluble powder or wetting powder, is made up of the component of following percetage by weight:
Lentinan 0.5-5%
Brassin lactones 0.0002-0.02%
Lauryl sodium sulfate 3%
White carbon 5-6%
NNO 5-10%
The kaolin surplus.
9. application according to claim 6 is characterized in that being made up of the component of following percetage by weight:
Lentinan 3-4%
Brassin lactones 0.01-0.02%
Ethanol 10%
Lauryl sodium sulfate 3%
Water surplus.
10. application according to claim 6 is characterized in that being made up of the component of following percetage by weight:
Lentinan 3-4%
Brassin lactones 0.01-0.02%
Lauryl sodium sulfate 3%
White carbon 6%
NNO 8%
The kaolin surplus.
11. according to each described application among the claim 6-10, it is characterized in that: the interval is 7-10 days said composition sprays twice, twice on crop between.
CN2011100278988A 2011-01-26 2011-01-26 Plant virus disease-resistant composition containing lentinan and brassinolide and application thereof Active CN102047883B (en)

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CN102826906B (en) * 2012-08-29 2014-04-16 广东植物龙生物技术有限公司 Composition comprising seaweed fertilizer and brassinolide
CN102823594B (en) * 2012-08-31 2014-07-09 广东植物龙生物技术有限公司 Plant virus disease resistance composition comprising diethyl aminoethyl hexanoate and lentinan
CN103918647A (en) * 2013-01-15 2014-07-16 中国农业科学院蔬菜花卉研究所 Biological pesticide dust agent
CN104871954B (en) * 2014-02-28 2017-06-27 卢德莉 A kind of breezing and producing method of red Chinese toon pollination liquid and red Chinese toon seed
CN109984133A (en) * 2019-04-03 2019-07-09 江西鑫邦科技有限责任公司 A kind of composition pesticide of the prevention and treatment virosis containing brassin lactones class compound
CN109938027A (en) * 2019-04-03 2019-06-28 江西鑫邦科技有限责任公司 A kind of composition pesticide of anti-plant viral disease and its preparation method and application
CN110896947A (en) * 2019-12-16 2020-03-24 江西省农业科学院植物保护研究所 Composition for preventing and treating plant virus diseases and preparation method and application thereof
CN111903702A (en) * 2020-08-14 2020-11-10 南京轩凯生物科技有限公司 Combined growth regulator containing pantoea polysaccharide and application thereof
CN115299445B (en) * 2022-10-08 2023-02-03 云南省烟草公司昆明市公司 Compound solution for improving induced disease resistance of lentinan to plants, preparation method and method thereof, and method for preventing and controlling viruses in plants in field

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CN101647438B (en) * 2009-09-07 2012-09-05 山东农业大学 Anti-plant virus composition containing lentinan
CN101869111B (en) * 2010-07-14 2012-10-31 山东圣鹏农药有限公司 Composition comprising lentinan and ribavirin for resisting plant virus diseases and application thereof
CN101941869B (en) * 2010-09-01 2013-08-28 山东圣鹏农药有限公司 Plant growth accelerator composition containing brassinolide and potassium dihydrogen phosphate and application
CN101953338A (en) * 2010-10-13 2011-01-26 山东农业大学 Plant cold-resistant antifreezing agent composition

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Address after: 272000 Yutun Town Residence, Rencheng District, Jining City, Shandong Province (Yutun Industrial Park)

Patentee after: SHANDONG SHENGPENG TECHNOLOGY CO.,LTD.

Address before: 272063 resident of yutun Town, Rencheng District, Jining City, Shandong Province

Patentee before: SHANDONG SHENGPENG PESTICIDE Co.,Ltd.

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Denomination of invention: Anti plant virus disease composition containing lentinan and brassinolide and its application

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