CN104430349A - Pesticide composition capable of preventing and treating plant virus diseases and application of pesticide composition - Google Patents
Pesticide composition capable of preventing and treating plant virus diseases and application of pesticide composition Download PDFInfo
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- CN104430349A CN104430349A CN201410802854.1A CN201410802854A CN104430349A CN 104430349 A CN104430349 A CN 104430349A CN 201410802854 A CN201410802854 A CN 201410802854A CN 104430349 A CN104430349 A CN 104430349A
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- plant
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Abstract
The invention relates to a pesticide composition capable of preventing and treating plant virus diseases and application of the pesticide composition, belongs to the pesticide compositions, and aims at providing a pesticide composition capable of preventing and treating plant virus diseases. The active components of the pesticide composition capable of preventing and treating plant virus diseases comprises lentinan and emodin monomethyl ether according to a weight ratio of (1-100) to (0.1-500). Furthermore, the invention also discloses application of the pesticide composition capable of preventing and treating plant virus diseases in prevention and treatment of plant virus diseases. According to the pesticide composition capable of preventing and treating plant virus diseases, lentinan and emodin monomethyl ether are compounded and used as active components; the components are environmentally friendly; the pollution to the environment is avoided; meanwhile, lentinan and emodin monomethyl ether have synergetic effects, have a good effect of preventing and treating the plant virus diseases and are capable of inducing and enhancing plant immunity, so that the infection of the virus can be inhibited; the effects of resisting virus and increasing yield can be achieved.
Description
Technical field
The present invention relates to the composition pesticide and application thereof of preventing and treating the viroses of plant, belong to composition pesticide.
Background technology
The viroses of plant are by the parasitogenic disease of plant virus.Plant virus must seek parasitic life in host cell, and specialization is strong, and a certain virus can only infect a certain or certain plants.The common harm widely viroses of plant has tobacco mosaic virus and cucumber mosaic virus.
General plant virus only in vivo just has activity host; Only minority plant virus can keep active several days, some months, even several years in the residual body of diseased plant, also has minority plant virus in vivo to survive insect or to breed.Plant virus carries out copying of nucleic acid (RNA or DNA) and protein coat in host cell, forms new virion.Plant virus plastochondria or viral nucleic acid transfer velocity between plant cell is very slow, then can shift rapidly with the nutrition flow direction of plant in vascular bundle, makes the morbidity of the plant whole body.
Application number be 201310322255.5 Chinese patent application disclose a kind of special effect agent preventing and treating plant virus and preparation method thereof, and specifically disclose described agent for prevention and control of virus and be made up of Moroxydine Hydrochloride, cantharidin, ammonium sulfate and DA-6, but its component is more complicated, cost is also higher.
Application number be 201310733788.2 Chinese patent application disclose a kind of the biogenic pesticide composition and the application thereof that prevent and treat the viroses of plant, the active component of described biogenic pesticide composition is made up of chitosan and lentinan, but its preventive effect is not satisfactory, only up to 82.5%.
Therefore, the composition pesticide preventing and treating the viroses of plant that in searching, component is simple, with low cost, preventive effect is excellent seems particularly important.
Summary of the invention
First technical problem that the present invention will solve is to provide a kind of composition pesticide preventing and treating the viroses of plant.
The present invention prevents and treats the composition pesticide of the viroses of plant, and its active component is lentinan and Physcion, wherein, by weight, and lentinan: Physcion=1 ~ 100:0.1 ~ 500; Be preferably, lentinan: Physcion=10 ~ 50:1 ~ 100; Be more preferably, lentinan: Physcion=1 ~ 50:1 ~ 10; Most preferably be, lentinan: Physcion=1 ~ 5:10.
Further, preferably, the present invention prevents and treats in the composition pesticide of the viroses of plant and also comprises auxiliary agent.The composition pesticide preferred dosage form that the present invention prevents and treats the viroses of plant is aqua, wetting powder, missible oil, microemulsion, suspending agent or water dispersible granules.
Preferably, the described content preventing and treating active component in the composition pesticide of the viroses of plant is 0.1 ~ 50wt%.
Preferably, the described composition pesticide aqueous emulsion preventing and treating the viroses of plant is made up of following parts by weight of component: lentinan 0.1 part, Physcion 50 parts, agriculture breast No. 500 2.8 parts, agriculture breast No. 601 7.2 parts, ethylene glycol 20 parts, 19.9 parts, water.
Further, the invention also discloses described prevent and treat the composition pesticide of the viroses of plant preventing and treating the application of the viroses of plant; Described virus is tobacco mosaic virus (TMV), Tomato mosaic virus (TOMV), cucumber mosaic virus (CMV), potato virus X (PVY).
Preferably, during use, being diluted with water to active component concentration is 10 ~ 100ppm, is preferably 50ppm.During use, sprayed by composition pesticide of the present invention in plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 7 ~ 10 days.
Compared with prior art, the present invention has the following advantages:
1, the present invention prevent and treat the viroses of plant composition pesticide adopt lentinan and Physcion composite as active component, each component is all environmentally friendly, can not cause environmental pollution.
2, in composition pesticide of the present invention, lentinan and Physcion have synergistic function, effective to Control of Plant Virus Disease, can induce and strengthen via plant immunity, thus suppress infecting of virus, reach antiviral and the effect of volume increase.
3, the composition pesticide that the present invention prevents and treats the viroses of plant can be prepared into the conventional types such as aqua, soluble powder or wetting powder, formulation variation, uses flexibly;
4, the composition pesticide that the present invention prevents and treats the viroses of plant has the effect of growth promoting effects, prevention and therapy virus disease concurrently, and cost performance is high, increases significant plant medical agent, and preparation method is simple, and raw material is easy to get, and has wide market prospects.
Embodiment
The present invention prevents and treats the composition pesticide of the viroses of plant, and its active component is lentinan and Physcion, wherein, by weight, and lentinan: Physcion=1 ~ 100:0.1 ~ 500.
Further, in order to improve the activity of composition pesticide of the present invention, preferably, in mass ratio, lentinan: Physcion=10 ~ 50:1 ~ 100; More preferably, in mass ratio, lentinan: Physcion=1 ~ 50:1 ~ 10; Most preferably, in mass ratio, lentinan: Physcion=1 ~ 5:10.
Further, in order to improve the activity of composition pesticide of the present invention, be convenient to store and transport simultaneously, the described composition pesticide preventing and treating the viroses of plant can add suitable auxiliary agent and be prepared into conventional any formulations of pesticide, makes aqua as added ethanol, lauryl sodium sulfate and water on the basis of active component; Add lauryl sodium sulfate, white carbon, dispersant NNO, kaolin makes soluble powder or wetting powder etc.
Wherein, preferably, reasonable formulation is preferably aqua, wetting powder, missible oil, microemulsion, suspending agent or water dispersible granules.
Further, preferably, the described content preventing and treating active component in the composition pesticide of the viroses of plant is 0.1 ~ 50wt%.
The Second Problem that the present invention will solve is to provide the above-mentioned application preventing and treating the composition pesticide of the viroses of plant.
The present invention prevent and treat the viroses of plant composition pesticide adopt lentinan and Physcion composite as active component, there is synergistic function, may be used for the disease that control plant causes due to virus, described virus is tobacco mosaic virus (TMV), Tomato mosaic virus (TOMV), cucumber mosaic virus (CMV), potato virus X (PVY) etc.
When the present invention prevents and treats the composition pesticide use of the viroses of plant, being diluted with water to active component concentration is 10 ~ 100ppm, is preferably 50ppm.During use, sprayed by composition pesticide of the present invention in plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 7 ~ 10 days.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 the present invention prevents and treats the preparation of the composition pesticide of the viroses of plant
Take each component according to component in table 1, mixing, obtains the composition pesticide that the present invention prevents and treats the viroses of plant.
Table 1
Test example 1 pharmaceutical composition of the present invention is used for the test of capsicum resisting tobacco mosaic virus disease
1 test material
1.1 for studying thing and object: eggplant door pimento tobacco mosaic virus (TMV)
1.2 medicament
Test medicine: XD-1 ~ XD-8;
Lentinan single dose (D1); Physcion single dose (D2);
Clear water contrast (CK).
Wherein, the preparation method of lentinan single dose (D1) is: get 2g lentinan, sodium lignin sulfonate 1kg, lauryl sodium sulfate 1kg, and mixing, complements to 100kg with potassium nitrate, make soluble powder.
The preparation method of Physcion single dose (D2) is: get 1g Physcion, adds agriculture breast 500#2.8kg, and agriculture breast 601#7.2kg, ethylene glycol 20kg, add water and mend to 100kg, make aqueous emulsion.
During use, above-mentioned medicament being diluted to active component concentration is 50ppm.
2, experimental scheme and method
Test method is with reference to NY/T2060.3-2011 " pepper disease resistance authenticate technology code the 3rd part: the sick authenticate technology code of capsicum resisting tobacco mosaic virus ".
Capsicum TMV virus disease: be the diseased plant caused by tobacco mosaic virus be system floral leaf, sick leaf is less than normal, slightly Zou's contracting; The stem of diseased plant, branch, petiole, carpopodium even there is the downright bad streak of long and short unsuitable brown in vein, fall leaves in a large number, only surplus minority blade time serious, are the most especially with pimento.There are yellow spot or necrotic plaque in sick fruit surface, and pulp is thinning, the pepper virus disease of the symptom such as to come off ahead of time.
Disease investigation:
Control time: carry out for 10 days after the 2nd dispenser.
State of an illness partition of the level:
0 grade: asymptomatic;
1 grade: the vein of lobus cardiacus is bright arteries and veins, or there is acute little withered spot in inoculation blade;
3 grades: the light floral leaf of minority blade, or inoculation leaf abscission, stem produces little necrotic spot;
5 grades: most blade floral leaf, minority leaf malformation, shrinkage, or stem produces downright bad streak;
7 grades: most blade is attached most importance to floral leaf, deformity, shrinkage, plant is downgraded, or stem, branch and vein have system necrotic plaque;
9 grades: most blade is attached most importance to floral leaf, deformity, shrinkage, plant is downgraded obviously; Or plant serious systemic is downright bad.
Investigation method:
Investigate every part of expert evidence inoculation strain incidence, describe according to Disease symptoms, investigate by part material, record state of an illness rank, calculate disease index PT according to formula 1:
PT＝[∑(N
i*i)/(N*9)]*100……(1)
In formula:
PT-disease index;
N
i-sick the number of sheets at different levels;
I-relative level numerical value;
N-investigate total number of sheets.
If carried out the investigation of state of an illness radix before dispenser, control efficiency has calculated by formula 2:
P＝[1-(CK
0/CK
1)*(PT
1/PT
0)]*100……(2)
P-control efficiency (%);
CK
0disease index before the dispenser of-blank district;
CK
1disease index after the dispenser of-blank district;
PT
0disease index before the dispenser of-chemicals treatment district;
PT
1disease index after the dispenser of-chemicals treatment district;
If do not carry out the investigation of state of an illness radix before dispenser, control efficiency calculates by formula 3:
P＝(1-PT
1/PT
0)*100…………(3)
Virus disease inoculates the last week, and once, latter one week of inoculation, repeats dispenser once, altogether dispenser twice in dispenser; Each experimental group formulation rate and disease index the results are shown in following table:
Table 2
| Treatment agent | Mu formulation rate | 10 days preventive effects (%) after second time dispenser |
| XD-1 | 30g | 83.64 |
| XD-2 | 30g | 81.2 |
| XD-3 | 30g | 93.82 |
| XD-4 | 30g | 86.91 |
| XD-5 | 30g | 86.7 |
| XD-6 | 30g | 88.2 |
| XD-7 | 30g | 93.82 |
Continued 2
| XD-8 | 30g | 80.9 |
| D1 | 30g | 57.45 |
| D2 | 30g | 33.27 |
| CK | 30g | —— |
As can be seen from the above table, adopt composition pesticide of the present invention to have higher preventive effect to capsicum tobacco mosaic virus, wherein, XD-3 and XD-7 pharmacy effect is best, and after secondary medicine, preventive effect all reaches 93.82%.Meanwhile, compared with D1, D2 single dose, its preventive effect significantly improves, visible, and composition pesticide of the present invention has synergistic function.
Test example 2 pharmaceutical composition of the present invention is used for the efficiency test of tomato virus disease
1 test material
1.1 for studying thing and object: tomato tomato virus disease (CMV)
1.2 medicament
Adopt with the medicament in test example 1.
2, experimental scheme and method
Test method is with reference to NYT1464.8-2007 " pesticide field efficacy medicine test criterion the 8th part: bactericidal agent for preventing and treating tomato virus disease ".
This test 4 medicaments, 2 single dose contrasts, 1 blank, repeats for four times, Gong32Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
With reference to example 1 investigation method, show that each experimental group formulation rate and disease index the results are shown in following table:
Table 3
| Treatment agent | Mu formulation rate | Preventive effect (%) |
| XD-1 | 30g | 83.77 |
| XD-2 | 30g | 87.30 |
| XD-3 | 30g | 93.83 |
| XD-4 | 30g | 91.01 |
| XD-5 | 30g | 92.53 |
Continued 3
| XD-6 | 30g | 80.26 |
| XD-7 | 30g | 96.27 |
| XD-8 | 30g | 79.2 |
| D1 | 30g | 60.85 |
| D2 | 30g | 67.55 |
| CK | 30g | —— |
As can be seen from Table 3, adopt composition pesticide of the present invention to have higher preventive effect to tomato virus disease, wherein, XD-7 pharmacy effect is best, and preventive effect reaches 96.27%.Meanwhile, compared with D1, D2 single dose, its preventive effect significantly improves, visible, and composition pesticide of the present invention has synergistic function.
Claims (10)
1. prevent and treat the composition pesticide of the viroses of plant, it is characterized in that: its active component is lentinan and Physcion, wherein, by weight, lentinan: Physcion=1 ~ 100:0.1 ~ 500.
2. the composition pesticide preventing and treating the viroses of plant according to claim 1, is characterized in that: in its active component, in mass ratio, lentinan: Physcion=10 ~ 50:1 ~ 100.
3. the composition pesticide preventing and treating the viroses of plant according to claim 2, is characterized in that: in its active component, in mass ratio, lentinan: Physcion=1 ~ 50:1 ~ 10; Be preferably lentinan: Physcion=1 ~ 5:10.
4. the composition pesticide preventing and treating the viroses of plant according to any one of claims 1 to 3, is characterized in that: described control in the composition pesticide of the viroses of plant also comprises auxiliary agent.
5. the composition pesticide preventing and treating the viroses of plant according to any one of Claims 1 to 4, is characterized in that: the described composition pesticide preventing and treating the viroses of plant is aqua, wetting powder, missible oil, microemulsion, suspending agent or water dispersible granules.
6. the composition pesticide preventing and treating the viroses of plant according to any one of Claims 1 to 5, is characterized in that: the described content preventing and treating active component in the composition pesticide of the viroses of plant is 0.1 ~ 50wt%.
7. the composition pesticide preventing and treating the viroses of plant according to claim 6, it is characterized in that: the described composition pesticide aqueous emulsion preventing and treating the viroses of plant is made up of following parts by weight of component: lentinan 0.1 part, Physcion 50 parts, agriculture breast No. 500 2.8 parts, agriculture breast No. 601 7.2 parts, ethylene glycol 20 parts, 19.9 parts, water.
8. described in any one of claim 1 ~ 7 prevent and treat the composition pesticide of the viroses of plant preventing and treating the application of the viroses of plant.
9. according to claim 8 prevent and treat the composition pesticide of the viroses of plant preventing and treating the application of the viroses of plant, it is characterized in that: described virus is tobacco mosaic virus (TMV), Tomato mosaic virus (TOMV), cucumber mosaic virus (CMV) or potato virus X (PVY).
10. according to claim 8 or claim 9 prevent and treat the composition pesticide of the viroses of plant preventing and treating the application of the viroses of plant, it is characterized in that: during use, being diluted with water to active component concentration is 10 ~ 100ppm, is preferably 50ppm.
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|---|---|---|---|
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Cited By (5)
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| CN106962349A (en) * | 2017-04-17 | 2017-07-21 | 广西南宁益土生物科技有限责任公司 | A kind of antiviral composition containing eugenol and lentinan |
| CN107360906A (en) * | 2017-08-04 | 2017-11-21 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of method for mitigating sweet potato SPVD harm |
| CN108338165A (en) * | 2017-03-31 | 2018-07-31 | 江苏辉丰农化股份有限公司 | A kind of microbicide compositions including emodin derivates and polysaccharide |
| CN108990989A (en) * | 2018-09-19 | 2018-12-14 | 西安绿海生物科技有限公司 | It is a kind of improve plant virus resistance ability immune inducing agent and its application |
| CN115299445A (en) * | 2022-10-08 | 2022-11-08 | 云南省烟草公司昆明市公司 | Compound solution for improving induced disease resistance of lentinan to plants, preparation method and method thereof, and method for preventing and controlling viruses in plants in field |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108338165A (en) * | 2017-03-31 | 2018-07-31 | 江苏辉丰农化股份有限公司 | A kind of microbicide compositions including emodin derivates and polysaccharide |
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| CN107360906A (en) * | 2017-08-04 | 2017-11-21 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of method for mitigating sweet potato SPVD harm |
| CN108990989A (en) * | 2018-09-19 | 2018-12-14 | 西安绿海生物科技有限公司 | It is a kind of improve plant virus resistance ability immune inducing agent and its application |
| CN115299445A (en) * | 2022-10-08 | 2022-11-08 | 云南省烟草公司昆明市公司 | Compound solution for improving induced disease resistance of lentinan to plants, preparation method and method thereof, and method for preventing and controlling viruses in plants in field |
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Application publication date: 20150325 |