CN105875622A - Pesticide composition for preventing tomato yellow leaf curl disease and preparation method thereof and application - Google Patents
Pesticide composition for preventing tomato yellow leaf curl disease and preparation method thereof and application Download PDFInfo
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- CN105875622A CN105875622A CN201610262471.9A CN201610262471A CN105875622A CN 105875622 A CN105875622 A CN 105875622A CN 201610262471 A CN201610262471 A CN 201610262471A CN 105875622 A CN105875622 A CN 105875622A
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- lentinan
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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Abstract
The invention relates to the field of pesticides and particularly discloses a pesticide composition for preventing tomato yellow leaf curl disease and a preparation method thereof and application. Active ingredients of the pesticide composition are matrine and lentinan. When the mass ratio of matrine and lentinan is 1-2:1-2, the pesticide composition has a remarkable synergistic effect on prevention of the tomato yellow leaf curl disease. In order to bring sterilization performances of the active ingredients into better play, the invention provides an aqueous solution pesticide composition which is composed of, by weight parts, 1-5 parts of matrine, 1-5 parts of lentinan, 1-5 parts of stabilizer, 1-5 parts of antifreezing agent and 80-96 parts of water. The pesticide composition improves disease resistance of plants, multiple effects of insect killing, sterilization and enhancement of plant disease resistance are embodied, and the pesticide composition has a very good synergistic effect on prevention of the tomato yellow leaf curl disease and is a very good agent for preventing the tomato yellow leaf curl disease.
Description
Technical field
The present invention relates to pesticide field, specifically, relate to preventing and treating tomato yellow leaf curl
Composition pesticide.
Background technology
Tomato yellow leaf curl (tomato yellow leaf curl disease, TYLCD) is kind
A kind of destructive disease on eggplant crop, the production loss caused can reach 100%.Cause
The pathogen of TYLCD be tomato yellow leaf curl virus (tomato yellow leaf curl virus,
TYLCV), this virus is mainly propagated by omnivorousness aleyrodid, belongs to geminivirus infection section.
TYLCV characteristics of incidence mainly has 2 points: after being Virus entry, performance is not sick
Levy, there is certain incubation period, become hidden symptom state, when environment is suitable, show symptom,
It is referred to as aobvious symptom state；Two is this disease and virosis (such as floral leaf, streak etc.) phase on other vegetables
For Bi, have burst, extension is rapid, hazardness is strong, treatment difficulty is big, is a kind of kind
Eggplant produces upper a kind of destructive vegetalitas disease.
The host main You Luo section of TYLCV, Compositae, pulse family, Malvaceae, Solanaceae, umbrella
Shape sections etc., mainly infect Solanaceae, especially Fructus Lycopersici esculenti.This disease symptom on Fructus Lycopersici esculenti mainly shows
For: Primary Production is slow or stagnates, delay of blooming, and flower reduces, and fruit reduces.Plant is bright
Aobvious downgrading, internode shortens, and blade diminishes, thickening, and the food value of leaf is brittle, and blade has rough
Shrinkage or deformation, upsweep, leaf near blade edge to the yellow of vein district, vein and vein
Color depth green glow is bright.Plant upper blade symptom is obvious, and bottom Lao Ye symptom is inconspicuous.Along with planting
The growth of strain, the state of an illness is increasingly severe, and the later stage mainly shows as vein purpling color, deformable blade,
Shrivelled, there is yellowish green uneven speckle, Post flowering result difficulty in young leaves, bears fruit few, and fruit diminishes,
Speed of expanding is slow, deformity.The fruit annesl in period of maturation is slow and uneven, and sarcocarp is hard, water content
Low, pulp acid, part cracking of fruit or surface brownization, lose commodity value.
TYLCV found in Israel first in nineteen thirty-nine～1940, and nineteen ninety-five, this disease was incoming
China, existing 15 provinces (autonomous region, municipality directly under the Central Government) in the whole nation find this disease.Since 2000,
This disease in Zhejiang, Chongqing, Guangxi, Guangdong, Yunnan, Shanghai, Shandong, the ground phase such as Henan
Secondary is raw, and tomato production causes serious threat, the danger to Fructus Capsici, Fructus Cucurbitae moschatae, Nicotiana tabacum L. etc.
Evil also has been reported that, according to the incomplete statistics of various places plant protection department, this disease is at present in China
Occurring area more than 200,000 hectares, year economic loss be up to billions of unit, and its occurring and damage
Just developed to face by point, sheet, to the Fructus Lycopersici esculenti industry of China's output value nearly hundred billion yuan be one the biggest
Threaten.And current, preventing and treating tomato yellow leaf curl mainly can rely on large-scale use chemical pesticide
Preventing and treating.Therefore, need a kind of Nantural non-toxic badly and can effectively prevent and treat the agriculture of tomato yellow leaf curl
Medicine.
Summary of the invention
In order to solve problems of the prior art, it is an object of the invention to provide a kind of preventing and treating
Composition pesticide of tomato yellow leaf curl and preparation method and application.
In order to realize the object of the invention, technical scheme is as follows:
First aspect, the present invention provides a kind of composition pesticide preventing and treating tomato yellow leaf curl,
Its active component includes matrine and lentinan.
Lentinan application agriculturally is mainly reflected in that it has well suppression to virus
And killing action, the resistance against diseases of plant can be improved simultaneously.Matrine is bent to propagating tomato yellowing
The aleyrodid of leaf disease has good insecticidal effect.The present invention has been surprisingly found that through experimental study, matrine
With lentinan compatibility, it is possible to Synergistic, tomato yellow leaf curl is realized significantly sterilization
Effect.
As preferably, The present invention gives the two feasible proportioning, i.e. matrine and lentinan
Mass ratio according to 1～5:1～5 forms.
More preferably, when the mass ratio of described matrine and lentinan is 1～2:1～2, two
The synergistic function of person becomes apparent from.
Composition pesticide of the present invention includes but are not limited to: water preparation and dissolvable agents, but is
Preferably play the bactericidal potency of active component, preferred water preparation and dissolvable agents.
When described composition pesticide is water preparation, in order to keep product stable, the present invention provides one
Plant preferable water preparation formula, be made up of the raw material of following weight portion: matrine 1-5 part, Lentinus Edodes
Polysaccharide 1-5 part, stabilizer 1-5 part, antifreezing agent 1-5 part, water 80-96 part.
As preferably, it is made up of the raw material of following weight portion: matrine 1-4 part, lentinan
1-4 part, stabilizer 2-5 part, antifreezing agent 2-5 part, water 82-94 part.
More preferably, be made up of the raw material of following weight portion: matrine 2 parts, lentinan 2
Part, stabilizer 4 parts, antifreezing agent 7 parts, deionized water 85 parts.
Wherein, one or more in ethylene glycol, propylene glycol and glycerol of antifreezing agent；Stable
Agent is carbamide or ethanol；Water is deionized water.Mentioned reagent is this area conventional reagent, belongs to
In commercially product, the present invention does not do restriction in detail.
Further, between preferred ingredient composition of the present invention and each component after proportioning, it is provided that one is
Good pesticide combination composition formula, it is made up of the raw material of following weight portion: matrine 2 parts, fragrant
Mushroom polysaccharide 2 parts, ethylene glycol 4 parts, 7 parts of carbamide, 85 parts of water.The present invention is through a large amount of objective tests
Finding, use this formula to prevent and treat tomato yellow leaf curl, effect is particularly excellent and notable.
Second aspect, the invention provides the preparation method of aforementioned composition pesticide (water preparation),
First by stabilizer and active component (matrine and lentinan) according to formula proportion mix and blend
Uniformly, mixing temperature controls at 25 DEG C-35 DEG C, and pH value controls at 5-7, adds antifreeze afterwards
Agent stirring 30min-1h, preparation overall process stirs under 60-80r/min rotating speed and carries out.
Method made above may insure that prepared water preparation has preferable water solublity and stablizes
Property.
The third aspect, present invention also offers aforementioned composition pesticide at preventing and treating tomato yellowing Qu Ye
Disease or be used in preparation preventing and treating tomato yellow leaf curl evil pesticide in application.
Preventing and treating tomato yellow leaf curl evil is particularly as follows: utilize described composition pesticide to obtain activity one-tenth
Point mass concentration is the water preparation of 4%, or yellow leaf curl disease morbidity early stage prosperous long-term at tomato plant,
Spray application on tomato leaf, sprayed once at interval of 8-15 days, spray 3-5 time.
The beneficial effects of the present invention is:
Matrine and lentinan compatibility are used by the present invention, can prevent disease from disease source
Occur.On the other hand, the resistance against diseases of plant is also improved.Embody parasite killing, sterilize, increase
The multi-efficiency of strong plant disease-resistant ability, has fabulous potentiation to imitate preventing and treating tomato yellow leaf curl
Really.The two is mixed have efficient, quick-acting, lasting period length, the Drug resistance that delays, simple to operate,
With low cost, to features such as person poultry safeties, be therefore extraordinary preventing and treating tomato yellow leaf curl
Medicament.The present invention confirms above-mentioned formula on the basis of lot of experiments is studied, this formula pair
The selection of active component and consumption and the selection of auxiliary agent and consumption thereof are really established a capital and are optimized
Screening, and has finally given a kind of preferable compound plant source pesticide, and its activity even can be with
Existing chemical pesticide is suitable.
The composition pesticide that the present invention relates to is active skull cap components formula, and various active composition is assisted
Same-action, is not likely to produce resistance, and in test, preventive effect is the highest can reach 85.71%, for organic
Agricultural provides efficient biological pesticide and selects.Additionally, composition pesticide of the present invention exists
Also having good stability during preservation, the shelf-life can reach 2 years.
Detailed description of the invention
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.Need
Being understood by providing merely to play descriptive purpose of following example, it is right to be not used to
The scope of the present invention limits.Those skilled in the art without departing substantially from spirit of the invention and
In the case of spirit, the present invention can be carried out various amendment and replacement.
Experimental technique used in following embodiment if no special instructions, is routine side
Method.
Material used in following embodiment, reagent etc., if no special instructions, all can be from business
Industry approach obtains.
Embodiment 1
Formula: matrine 2g, lentinan 2g, ethylene glycol 4g, carbamide 7g, water 85g.
Preparation method:
First require to add in preparation still according to formula proportion by stabilizer and active component, prepared
Journey stirs, and mixing temperature controls at 25 DEG C-35 DEG C so that active component fully dissolves, and controls
PH value at about 5-7, keeps active component stablizing, to ensure the matter of product in the solution
Amount.Being eventually adding antifreezing agent, be stirred for 30min-1h, preparation overall process is at 60-80r/min
Under rotating speed, stirring is carried out.After having prepared, product is carried out active component content control, content
Detect qualified rear packaging, i.e. prepare 4% aqueous pesticide compositions.
Embodiment 2
The present embodiment is with the difference of embodiment 1, and formula is: matrine 1g, and Lentinus Edodes is many
Sugar 3g, propylene glycol 6g, carbamide 6g, water 84g.
Embodiment 3
The present embodiment is with the difference of embodiment 1, and formula is: matrine 3g, and Lentinus Edodes is many
Sugar 4g, glycerol 5g, ethanol 6g, water 82g.
Embodiment 4
The present embodiment is with the difference of embodiment 1, and formula is: matrine 2g, and Lentinus Edodes is many
Sugar 4g, ethylene glycol 6g, ethanol 6g, water 82g.
Embodiment 5-8
It is likewise possible to the pesticide group of following different formulations can be prepared according to embodiment 1 method
Compound:
The formula of embodiment 5-8 is as follows:
| Embodiment | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 |
| Matrine | 2 | 5 | 3 | 4 |
| Lentinan | 5 | 1 | 5 | 5 |
| Antifreezing agent | Propylene glycol 3 | Glycerol 2 | Ethylene glycol 6 | Glycerol 4 |
| Stabilizer | Ethanol 6 | Carbamide 7 | Carbamide 6 | Ethanol 8 |
| Water | 84 | 85 | 80 | 79 |
Experimental example 1
This experimental example is for verifying the performance of plant pesticide composition of the present invention.
As a example by embodiment 1, verify the gained water preparation preparation field medicine to tomato yellow leaf curl
Effect.
Spraying time: prosperous for a long time at tomato plant, the morbidity early stage medication first of yellow leaf curl disease,
At interval of medication in about 10 days the most once, 3 times are sprayed continuously.After last dispenser 10
My god, a situation arises for investigation tomato yellow leaf curl, and classification is recorded.
Investigation method: every random 5 samplings in community, every randomly selects 10 strains, and investigation is every
Whole blades of strain, every community investigates 50 strains, and in units of strain, total strain number, each investigated in record
Level diseased plant number.
Grade scale:
0 grade: without any symptom；
1 grade: newborn blade is little, has aetiolation；
3 grades: newborn blade and 4-6, top yellowing leaf, and roll up on edge；
5 grades: upper blade yellow, blade is little, and plant strain growth is slow；
7 grades: middle and upper part yellowing leaf, growth stops, hence it is evident that downgrading；
9 grades: plant strain growth stops, downgrading, Post flowering is beared fruit difficulty, and fruit is little, and fruit is abnormal
Shape.
Computational methods:
In formula: Pt-chemicals treatment disease refers to, CK-clear water comparison disease refers to.
Table 1 embodiment 1 gained 4% water preparation preventing and treating Fructus Lycopersici esculenti Hemerocallis citrina Baroni leaf curl result of the test
As shown in Table 1, tomato yellow leaf curl is had well by embodiment 1 composition pesticide
Preventive effect, effect can match in excellence or beauty with related chemistry pesticide.Compared with chemical bactericide, institute of the present invention
State composition pesticide and take natural active component completely, there are wide material sources, cheap,
The more important thing is that said composition applies the not side effect such as residual, it is ensured that food safety, have
It is widely applied prospect.
Comparative example 1
This comparative example is for proving the synergistic function of matrine and lentinan.
Experimental group 1: formula: lentinan 4g, ethylene glycol 4g, carbamide 7g, water 85g.
Preparation method is with embodiment 1.
Experimental group 2: formula: matrine 4g, ethylene glycol 4g, carbamide 7g, water 85g.System
Preparation Method is with embodiment 1.
Experimental group 3: embodiment 1.
Experimental group 1-2 carries out prevent and treat tomato yellow leaf curl test test, statistical result, as
Shown in table 2-3.
Table 2 experimental group 1 gained 4% lentinan water preparation preventing and treating tomato yellow leaf curl result of the test
Table 3 experimental group 2 gained 4% aqueous matrine solution preventing and treating tomato yellow leaf curl result of the test
By contrast table 1, table 2, table 3, matrine or the single formula of lentinan
Preventing and treating tomato yellow leaf curl effect is all not as the prevention effect of formula of test example 1 of the present invention.
Comparative example 2
This comparative example is for investigating the different ratio between matrine and lentinan to synergism
Impact.
This comparative example carries out the efficiency test of Fructus Lycopersici esculenti Hemerocallis citrina Baroni leaf curl to embodiment 1-8, and result is such as
Shown in table 4.
The impact on the preventive effect of Fructus Lycopersici esculenti Hemerocallis citrina Baroni leaf curl of the table 4 embodiment 1-8 different ratio composition pesticide
As shown in Table 4, under same active component dosage, lentinan and matrine
When proportioning is 1:1, both synergism are best.Two respective advantages of active component can
To obtain maximum performance.Meanwhile, proportioning 1:1 is from the point of view of reality application angle, the most both economical.
Although, the most with a general description of the specific embodiments the present invention has been made in detail
Most description, but on the basis of the present invention, it can be made some modifications or improvements, this is to this
It is apparent from for skilled person.Therefore, on the basis without departing from spirit of the present invention
Upper these modifications or improvements, belong to the scope of protection of present invention.
Claims (10)
1. the composition pesticide preventing and treating tomato yellow leaf curl, it is characterised in that it is lived
Property composition includes matrine and lentinan.
Composition pesticide the most according to claim 1, it is characterised in that described pesticide
The active component of compositions is made up of according to the mass ratio of 1～5:1～5 matrine and lentinan.
Composition pesticide the most according to claim 2, it is characterised in that described Radix Sophorae Flavescentis
The mass ratio of alkali and lentinan is 1～2:1～2.
4. according to the composition pesticide described in any one of claim 1-3, it is characterised in that institute
The dosage form stating composition pesticide is water preparation.
Composition pesticide the most according to claim 4, it is characterised in that it is by weighing as follows
The raw material composition of amount part: matrine 1-5 part, lentinan 1-5 part, stabilizer 1-5 part, antifreeze
Agent 1-5 part, water 80-96 part.
Composition pesticide the most according to claim 5, it is characterised in that it is by weighing as follows
The raw material composition of amount part: matrine 2 parts, lentinan 2 parts, stabilizer 4 parts, antifreezing agent 7
Part, deionized water 85 parts.
7. according to the composition pesticide described in claim 5 or 6, it is characterised in that antifreezing agent
One or more in ethylene glycol, propylene glycol and glycerol；Stabilizer is carbamide or second
Alcohol；Water is deionized water.
Composition pesticide the most according to claim 7, it is characterised in that it is by weighing as follows
The raw material composition of amount part: matrine 2 parts, lentinan 2 parts, ethylene glycol 4 parts, 7 parts of carbamide,
85 parts of water.
9. the preparation method of composition pesticide described in any one of claim 1～8, its feature exists
In, first by stabilizer and active component according to formula proportion mixing and stirring, mixing temperature control
System is at 25 DEG C-35 DEG C, and pH value controls at 5-7, adds antifreezing agent stirring 30min-1h afterwards,
Preparation overall process stirs under 60-80r/min rotating speed and carries out.
10. composition pesticide described in any one of claim 1～8 is at preventing and treating tomato yellow leaf curl
The application of aspect.
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| CN201610262471.9A CN105875622B (en) | 2016-04-25 | 2016-04-25 | A kind of composition pesticide for preventing and treating tomato yellow leaf curl and preparation method and application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770879A (en) * | 2018-06-05 | 2018-11-09 | 蚌埠市刘兴桃种植农民专业合作社 | A kind of pesticide and preparation method thereof of prevention tomato yellow leaf curl |
| CN109497095A (en) * | 2019-01-03 | 2019-03-22 | 临沂大学 | A kind of biological pesticide |
| CN112125980A (en) * | 2020-08-31 | 2020-12-25 | 中国科学院华南植物园 | Opuntia ficus-indica acidic polysaccharide with antiviral effect, and preparation method and application thereof |
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| CN101081240A (en) * | 2006-05-30 | 2007-12-05 | 黄振华 | Medicinal composition of oxymatrine and polysaccharide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108770879A (en) * | 2018-06-05 | 2018-11-09 | 蚌埠市刘兴桃种植农民专业合作社 | A kind of pesticide and preparation method thereof of prevention tomato yellow leaf curl |
| CN109497095A (en) * | 2019-01-03 | 2019-03-22 | 临沂大学 | A kind of biological pesticide |
| CN112125980A (en) * | 2020-08-31 | 2020-12-25 | 中国科学院华南植物园 | Opuntia ficus-indica acidic polysaccharide with antiviral effect, and preparation method and application thereof |
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