KR102243071B1 - composition for preventing Tomato yellow leaf curl virus disease and promoting growth of tomato, tomato cultivating method using the same - Google Patents

Abstract

The present invention relates to a composition for promoting tomato growth and controlling sulfide virus, and a method of cultivating tomato using the same, and more particularly, containing riboflavin and baekduong extract to promote the growth of tomatoes and have an effect on the control of tomato sulfide virus. The present invention relates to a cultivation method capable of increasing the content of riboflavin contained in fruits while promoting the growth of tomatoes by cultivating tomatoes using the composition and at the same time.

Description

Composition for preventing Tomato yellow leaf curl virus disease and promoting growth of tomato, tomato cultivating method using the same}

The present invention relates to a composition for promoting tomato growth and controlling sulfide virus, and a method of cultivating tomato using the same, and more particularly, containing riboflavin and baekduong extract to promote the growth of tomatoes and have an effect on the control of tomato sulfide virus. The present invention relates to a cultivation method capable of increasing the content of riboflavin contained in fruits while promoting the growth of tomatoes by cultivating tomatoes using the composition and at the same time.

About 2,000 species of plant viruses have been isolated and reported worldwide, and about 120 species of plant viruses have been reported in Korea. Plant viruses cause diseases in many plants and cause serious economic damage throughout agricultural production, such as a decrease in the production of various crops and flowers, decrease in quality, and deterioration of varieties.

In particular, as the volume of international trade in food and various plants has increased in recent years, foreign pathogenic plant viruses that did not exist in the country have been introduced, and the resulting damage is gradually increasing. Therefore, the prevention, treatment and quarantine of viruses on cultivated plants is an important task in agriculture.

Tomato yellow leaf curl virus (TYLCV), a kind of tomato sulfide virus, is a DNA virus belonging to the genus Geminiviridae and Begomovirus, and is a plant virus having a circular single-stranded DNA genome (ssDNA) of 27 kb (Brunt and Cohen). , 1988; Lee et al, 2010).

TYLCV is one of the plant viruses that occurred in the Middle East in the 1960s, and is now occurring almost all over the world, causing serious economic damage. It is mainly spread by the medium of cigarette powder (Whitefly). It occurs in various tomato species, and the growth point of tomatoes is yellowed at the beginning of the onset and the leaves are dried. After the onset, the growth of shoots stops, and if it occurs during the growth, the fruiting rate decreases, causing a serious loss in tomato production.

In recent years, research on plant extracts with anti-plant virus active substances is active.

Plant virus control composition containing the blackcurrant extract of Korean Patent No. 10-1457869, the plant virus control composition containing the oak tree larva extract of Registration Patent No. 10-1287490, and the five baeja extract of Registration Patent No. 10-0738647 Various plant extracts, such as plant virus control agents, are used as control agents.

However, in the case of tomato sulfide virus, only studies on the development of tomato cultivar resistant to viruses or studies for detection or diagnosis of viruses have been focused on, but studies on the control of tomato sulfide virus are still insignificant.

1. Republic of Korea Patent Registration No. 10-1457869: Plant virus control composition containing blackcurrant extract 2. Republic of Korea Patent Registration No. 10-1287490: Plant virus control composition containing the oak tree lodge extract 3. Republic of Korea Patent Registration No. 10-0738647: Plant virus control agent containing five baeja extract

The present invention was created to improve the above problems, and promotes the growth of tomatoes by containing riboflavin and baekduong extract, and provides a composition effective in controlling tomato sulfide virus, and at the same time, the growth of tomatoes by cultivating tomatoes using the same Its purpose is to provide a cultivation method capable of increasing the content of riboflavin contained in fruits with promotion.

The composition for promoting tomato growth and controlling yellowing virus of the present invention for achieving the above object contains riboflavin and baekduong extract, and has the effect of promoting growth of tomato and controlling yellowing virus.

The Baek Duong extract is extracted with at least one extraction solvent selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof.

And the tomato cultivation method of the present invention for achieving the above object is to cultivate the tomato by spraying the above composition.

The fruit of the tomato has an increased content of riboflavin.

As described above, the composition of the present invention contains riboflavin and baekduong extract to promote the growth of tomatoes and at the same time have excellent control effects against tomato sulfide virus.

In addition, since the content of riboflavin contained in fruits can be greatly increased during tomato cultivation by using the composition of the present invention, a tomato having excellent functionality can be produced.

1 to 5 are photographs showing drug efficacy and ineffectiveness test results,
6 to 8 are graphs showing the results of the growth test on the 30th day after planting according to the farmhouse proof packaging test,
9 to 11 are graphs showing the results of the growth test on the 50th day after planting according to the farmhouse proof packaging test,
12 to 14 are graphs showing the results of the control on the 50th day after planting according to the farmhouse proof packaging test,
15 is a test report showing the results of riboflavin content analysis of the untreated group,
16 is a test report showing the results of the riboflavin content analysis of the treatment group.

Hereinafter, a composition for promoting tomato growth and controlling yellowing virus according to a preferred embodiment of the present invention and a method of cultivating a tomato using the same will be described in detail.

The composition for promoting tomato growth and controlling yellowing virus of the present invention contains riboflavin and baekduong extract.

For example, the composition may contain 20 to 80% by weight of a riboflavin solution in which riboflavin is dissolved, and 20 to 80% by weight of a baekduong extract extracted from baekduong.

Riboflavin can be dissolved in water to obtain a riboflavin solution. For example, it can be dissolved by adding 0.01 to 10 parts by weight of riboflavin based on 100 parts by weight of water.

Baekduong extract is extracted from Baekduong.

Baek Duong is the herbal name for Pulsatilla Koreana , which also refers to only the root of the Paekmi flower. About 30 species of pasqueflowers grow wild in East Asia and Europe, and the leaves of pasqueflowers are gathered at the roots, and they are deeply divided like birds' hairs. The flowers are reddish purple and bloom in April-May, and white long hairs are attached.

Baekduong, the root of pasqueflower, is rich in saponin, a compound of hederagenin glucoside, and has been used as a medicinal for pain relief, hemostasis, anti-inflammation, geonwi, ichthyosis, pungsang, birthgi, convergence, dysentery, branch, neuralgia, etc. In addition, it is a medicinal plant with a variety of uses, such as using the insecticidal effect of the roots as a repellent.

Baekduong extract can be extracted in a variety of ways. For example, after adding an extraction solvent to Baekduong by weight ratio of 2 to 40 times, it may be extracted by hot water extraction, cold needle extraction, or hot needle extraction at 10 to 150°C for 1 to 40 hours.

As the extraction solvent, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used. Methanol, ethanol, etc. can be used as the lower alcohol having 1 to 4 carbon atoms, and butylene glycol, propylene glycol, pentylene glycol, glycerol, and the like can be used as the polyhydric alcohol. And as the mixture, a mixture of water and a lower alcohol, a mixture of water and a polyhydric alcohol, a mixture of a lower alcohol and a polyhydric alcohol, or a mixture of water and a lower alcohol and a polyhydric alcohol may be used.

In addition to the liquid extract, the Baek Duong extract may also include active fractions obtained through various purification methods additionally performed, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography, and the like.

The composition for promoting tomato growth and controlling yellowing virus of the present invention may be a mixture of a riboflavin solution and Baekduong extract, or may further include a known excipient in the mixture. For example, a solid carrier, a liquid carrier, a liquid diluent, a liquefied gas diluent, a solid diluent, or other suitable auxiliaries, such as an emulsifier, a dispersant, or a foaming agent, may be further included as known excipients.

The composition for promoting tomato growth and controlling yellowing virus of the present invention may be formulated and used in various formulations known in the agrochemical field, and any formulation method commonly used in the agrochemical field may be used for formulation. For example, it may be formulated as a wettable powder, granule, powder, emulsion, spray, smoke, capsule and gel.

The composition for promoting tomato growth and controlling yellowing virus of the present invention may be used as an undiluted solution or diluted 100 to 1000 times, and the concentration may be appropriately adjusted in consideration of the degree of growth, the environment of the cultivated land, and the degree of onset of plant diseases. .

The composition for promoting tomato growth and controlling yellowing virus of the present invention can promote growth by spraying treatment on the soil in which tomatoes are growing or leaves of growing tomatoes during cultivation of tomatoes. In addition, the present invention can control diseases caused by plant viruses. In particular, the present invention has excellent control effects against tomato yellow leaf curl virus (TYLCV) as a plant virus.

By using the composition for promoting tomato growth and controlling yellowing virus of the present invention, it is possible to greatly increase the content of riboflavin contained in fruits along with the effect of promoting growth and controlling when growing tomatoes.

Riboflavin, called vitamin B2, is one of the important vitamins involved in various metabolism. Vitamin B2 works to reduce triglycerides and cholesterol. In addition, another function of vitamin B2 is to prevent obesity, such as inhibiting the production of lipid peroxide. Lack of vitamin B2 can cause stomatitis, stomatitis, glossitis, seborrheic dermatitis, dry eye, red eye, cataract, and anemia.

By increasing the riboflavin content of tomato fruit, it is useful for health when eating fruit.

The composition for promoting tomato growth and controlling yellowing virus of the present invention can be cultivated by spraying on the foliar surface at intervals of 5 to 10 days during the growth period of the tomato. The amount of riboflavin contained in the tomato fruit grown in this way is increased by about 50% compared to the content of riboflavin contained in tomato fruit grown by a conventional method.

Hereinafter, the present invention will be described through examples. However, the following examples are for explaining the present invention in detail, and are not intended to limit the scope of the present invention to the following examples.

(Example)

1. Riboflavin solution

A riboflavin solution was obtained by dissolving 1.0 part by weight of riboflavin based on 100 parts by weight of water.

2. Baekduong extract

The dried baekduong was pulverized to a particle size of 100 mesh, water was added 20 times by weight, heated at 80° C. for 8 hours, and filtered to obtain a liquid baekduong extract.

3. Composition

The prepared riboflavin solution and Baekduong extract were mixed in a weight ratio of 1:1 to prepare a composition for promoting tomato growth and controlling sulfur virus.

<Pharmaceutical and Ineffective Test>

In order to evaluate the efficacy of the composition against tomato growth promotion and tomato sulfide virus, drug efficacy and ineffectiveness tests were performed.

In order to induce tomato sulfide virus disease in tomatoes as a test subject, a method of artificially inoculating the powdered powdered leaves of tomatoes infected with tomato yellow leaf curl virus (TYLCV) into the test subject tomato seedlings was chosen. And Pseudomonas chlororaphis known to have antiviral activity as a positive control was used.

1. Test method

end. Subject: Tomato growth promotion effect, tomato sulfur virus

I. Test crop (variety): Tomato (drop tomato)

Everything . Target Disease Occurrence Situation: Tomato seedlings are purchased and infected powders infected with tomato yellowing virus are artificially inoculated and tested. The incidence rate of tomato sulfide virus in the untreated group was 71.85% on average, indicating that it was sufficient for the weak test.

D. Ineffectiveness test: Investigation of fresh weight and dry weight of tomato seedlings

hemp. Processing contents

The experimental group was treated with the composition of Example 1, the comparative group 1 was treated with the riboflavin solution of the example, and the comparative group 2 was treated with Baekduong extract. And Pseudomonas chlororaphis (Pseudomonas chlororaphis) fungicide culture solution was treated as a positive control, and water was treated as a negative control. The treatment contents are summarized in Table 1 below.

division Test drug Dilution factor Treatment method Comparative phrase 1 Riboflavin solution 500 times

Foliar spraying 3 times at 7 days intervals, Comparative phrase 2 Baekduong extract 100 times Experiment Composition 500 times Positive control Pseudomonas chlororapis culture solution 100 times No treatment area (voice control area) water -

2. Investigation method

The investigation method is shown in Table 2 below.

division Survey item Number of investigations Investigation method Drug efficacy test Incidence 1 time 5-week incidence survey per treatment area Ineffectiveness test Live weight, dry weight 1 time 5-week growth rate survey per treatment area

The test results are shown in Tables 3 and 4, and FIGS. 1 to 5 below. FIG. 1 is a picture of Comparative Group 1, FIG. 2 is a picture of Comparative Group 2, FIG. 3 is a picture of an experimental group, FIG. 4 is a picture of a positive control group, and FIG. 5 is a picture of an untreated group.

3. Drug efficacy test results

The results of the drug efficacy test 7 days after the final treatment of the test drug are shown in Table 3 below.

division Incidence (%)
Control (%) Repeat 1 2 repetitions 3 repetitions Average Comparative phrase 1 32.33±0.58 30.11±1.0 34.26±1.15 33.33±1.28 53.5±1.74 Comparative phrase 2 37.57±0.58 38.16±0.58 36.49±1.15 37.41±1.31 47.9±3.86 Experiment 17.78±0.58 15.56±0.58 11.11±0.58 14.81±1.96 79.0±3.77 Positive control 24.44±1.0 26.67±0.58 28.89±1.53 26.67±1.28 62.8±0.79 No treatment 66.67±0.58 66.89±1.53 80.0±0.58 71.85±4.12 -

Referring to the results in Table 3, the incidence (area area) of the untreated group was 71.85%, which was sufficient to investigate the drug efficacy against tomato sulfide virus. The experimental group showed a control effect of 79.0% compared to the untreated group. This is a remarkably increased result compared to Comparative Group 1 and Comparative Group 2. Through this, it was found that using a mixture of the two rather than using riboflavin or Baek Duong extract alone has a great synergistic effect in terms of the control effect.

4. Ineffective test results

The results of the ineffectiveness test 7 days after the final treatment of the test drug are shown in Table 4 below.

division Live weight Out of the building Biomass (g) Indices(%) Dry matter (g) Indices(%) Comparative phrase 1 136.6 ^b 113.46 7.88 ^b 110.36 Comparative phrase 2 128.6 ^b 106.81 7.48 ^b 104.76 Experiment 142.6 ^c 118.44 8.32 ^c 116.53 Positive control 133.6 ^b 110.96 7.68 ^b 107.56 No treatment 120.4 ^a 100 7.14 ^a 100

Referring to the results of Table 4, it was confirmed that the biomass increased by about 18.44% in the experimental group compared to the untreated group, and about 16.53% increased in the experimental group compared to the untreated group even when comparing dry weight.

<Drug efficacy and weakness test>

The composition of the example was named'BioDoctor Plus', and in order to test the efficacy of the product, an experiment on tomato sulfur virus was requested to the (Re) Eco-friendly Agricultural Life Research Center, an authorized testing institution.

1. Test method

end. Target disease: Tomato yellow leaf curl virus (TYLCV)

I. Test crop (variety): Tomato (drop tomato)

Everything . Target disease occurrence situation: The incidence of untreated groups was 42.41%, which was sufficient for drug efficacy review.

la. Contents of treatment: Tomato sulfur virus drug was not separately registered, so there was no separate control. The treatment contents are shown in Table 5 below.

Disclosure Fee Medicinal effect Weak exam
Year Processing time and method Reference quantity Double Reference quantity Double Bio Doctor Plus 1000 times 500 times 1000 times 500 times 1st year Foliar spraying three times every 7 days during growth after planting (4/12, 4/19, 4/26) No treatment - - - - - -

hemp. Overview of seedlings: House facility cultivation, planting interval 40cm, no other drugs during the test period, seedling official date: March 18

bar. Arrangement and area of test area: 3 repeats of egg mass method

ｏ Ineffective: 3 repetitions with a total of 3 treatments: no treatment, quantification of treatment, and ration of treatment

ｏ Comparison: Quantification of treatment zones, doubling of treatment zones, 3 repetitions with a total of 2 treatment zones

The test area layout and area are shown in Table 6 below.

division Treated water Number of repetitions Total number Gudang area Total area required Medicinal effect 3 3 9 20m ² 180m ² Weak 2 3 6 5m ² 30m ²

four. Weather conditions before and after spraying of the publicly disclosed sample: cultivation of house facilities does not affect ineffectiveness and comparison. The weather conditions are shown in Table 7 below.

Month/day Rainfall (mm) Highest/lowest temperature (℃) Average temperature (℃) ^● March 18 - 13.8/2.2 6.6 March 19 - 19.4/-0.7 10.5 * April 12 - 17.7/2.0 10.3 April 13 - 18.1/1.8 9.5 * April 19 - 17.7/9.9 13.3 April 20 - 22.6/7.2 14.5 * April 26 0.2 13.0/8.0 9.7 April 27 - 18.4/5.3 11.2

( ^● official date, *: drug treatment date)

2. Investigation method

end. Medicinal efficacy: Total incidence survey of survey specimens

I. Weakness: Investigation of whether there is any weakness in appearance

The investigation method is shown in Table 8 below.

division Survey item Number of investigations Date of investigation Investigation method Drug efficacy test Leaf length, leaf width,
Live weight, dry weight 1 time 5/10 Investigation for 20 weeks per ward 14 days after the last drug treatment Weakness test In appearance
Weakness or not 3rd time 4/15, 4/17, 4/19 3, 5, 7 days after initial drug treatment

3. Test results

end. Tomato sulfur virus drug efficacy test result (14 days after treatment with test drug)

The drug efficacy test results are shown in Table 9 below.

Test drug Incidence (%) valence Control (%) Repeat 1 2 repetitions 3 repetitions Average Bio Doctor Plus 20.0 20.56 13.33 17.96±2.32 a 57.65 No treatment 43.33 42.22 41.67 42.41±0.49 b 0

Referring to Table 9, the onset of 42.41% or more in the untreated group was sufficient to investigate the drug efficacy, and BioDoctor Plus showed a control effect of 57.65% compared to the untreated group. Therefore, it was confirmed that the control effect of BidoDoctor Plus was excellent.

I. Weakness test result

The results of the weakness test are shown in Table 10 below.

Disclosure Fee Number of investigations Date of investigation Weak degree (0~5) Remark Reference quantity Double
Baido Doctor Plus
3 April 15 0 0
No weakness April 17 0 0 April 19 0 0

Referring to the results of Table 10, it was found that there was no weakness in tomatoes when treated with a reference amount and a double amount of the test sample.

<Farmhouse proof packaging efficacy test>

The experiment was conducted by visiting the field where the disease was caused by tomato yellow leaf curl virus (TYLCV).

In addition, through an in-flight experiment, BioDoctor Plus and BioDoctor were compared to analyze the effect of improving the growth of crops and the effect on tomato sulfur virus, and the riboflavin content was requested to be tested by an accredited testing agency. The'biodoctor' described below means the riboflavin solution used in the examples.

1. Packing location: Cherry tomato farm located in Pyeong-dong, Gwangsan-gu, Gwangju

2. Purpose of the experiment: Through an experiment that compares BioDoctor Plus in which the riboflavin solution and Baekduong extract are mixed in a weight ratio of 1:1 with the biodoctor consisting only of riboflavin solution, the effect of promoting crop growth and controlling effect against tomato yellowing virus and This experiment was conducted to investigate the riboflavin content contained in the final fruit.

3. Cultivated crops: Cherry tomatoes

4. Treatment zone setting: No treatment zone, BioDoctor treatment zone, and BioDoctor Plus treatment zone, and 3 repetitions were performed for each treatment zone.

5. Treatment method

-Processing unit repetition number: 3 repetitions with random block designation

-Product spraying frequency: 3 times treatment

-Treatment area area: 30m ^{2 for each treatment area}

-Treatment method: foliar spray

-Official Date: 2019-03-04

-Processing time:

1st processing: 2019-03-18, 2nd processing: 2019-03-25, 3rd processing: 2019-04-15

-Survey period:

Growth survey was conducted on April 03, 2019 (30 days after planting) and April 23, 2019 (50 days after planting), and control investigation was conducted on April 23, 2019 (50 days after planting).

4. Test result

(1) Growth survey

The results of the growth survey on the 30th day after planting are shown in FIGS. 6 to 8 and Table 11 below.

division No treatment Bio Doctor Bio Doctor Plus plant height(cm) 158.04±1.06 166.43±1.84 171.73±1.59 number of flowers/plant 5.38±0.06 5.80±0.23 6.10±0.22 number of fruits/flowers 6.68±0.19 8.23±0.15 8.63±0.09

6 to 8 and referring to the results of Table 11, it was found that the growth of the treatment group treated with BioDoctor and BioDoctor Plus was improved compared to the untreated group. In particular, in the case of BioDoctor Plus, it was found to be excellent in fresh weight, flower water, and fruit water.

The results of the growth survey on the 60th day after planting are shown in FIGS. 9 to 11 and Table 12 below.

division No treatment Bio Doctor Bio Doctor Plus brix(%) 158.04±1.06 166.43±1.84 171.73±1.59 fruits diameter(mm) 5.38±0.06 5.80±0.23 6.10±0.22 yield(kg/plot) 6.68±0.19 8.23±0.15 8.63±0.09

Referring to the results of FIGS. 9 to 11 and Table 12, it was found that the sugar content, fruit size, and yield of the treated groups treated with BioDoctor and BioDoctor Plus were all improved compared to the untreated groups. In particular, in the case of BioDoctor Plus, it was confirmed that the test results were the best.

(2) control

The results of the control test on the 50th day after planting are shown in FIGS. 12 to 14 and Table 13 below. Fig. 12 is a result of the control in one treatment, Fig. 13 is a result of the control in two treatments, and Fig. 14 is a result of the control in three treatments. In FIGS. 12 to 14, the black graph represents the incidence (%), and the white graph represents the control (%).

division No treatment Bio Doctor Bio Doctor Plus Controlling once treatment - 42.4±0.6 47.6±1.1 Controlling in two treatments - 46.3±0.6 51.5±1.1 Controlling in 3 treatments - 54.1±1.4 57.6±2.3

Referring to the results of FIGS. 12 to 14 and Table 13, it was found that BioDoctor Plus has superior control over that of BioDoctor.

(3) Riboflavin content

The fruits of tomatoes grown by treatment three times with untreated and BioDoctor Plus were submitted to KOTITI Testing and Research Institute to analyze the content of riboflavin. The analysis results are shown in FIGS. 15 and 16. Fig. 15 is a test report of the untreated group, and Fig. 16 is a test report of the BioDoctor Plus treatment group.

15 and 16, it was found that the content of riboflavin contained in the fruit was increased by about 50% in the treated group.

In the above, the present invention has been described with reference to an exemplary embodiment, but this is only exemplary, and those of ordinary skill in the art will appreciate that various modifications and equivalent embodiments are possible therefrom. Therefore, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (4)

It contains 20 to 80% by weight of riboflavin solution and 20 to 80% by weight of Baekduong extract,
It has the effect of promoting tomato growth and controlling sulfur virus,
The sulfide virus is a tomato sulfide leaf curl virus (Tomato yellow leaf curl virus; TYLCV) A composition for promoting tomato growth and controlling sulfide virus, characterized in that. The composition of claim 1, wherein the Baek Duong extract is extracted with at least one extraction solvent selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, or mixtures thereof. . A tomato cultivation method, characterized in that the tomato is cultivated by spraying the composition of claim 1 or 2. The method of claim 3, wherein the tomato fruit has an increased riboflavin content.

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