KR101484301B1 - rice plant cultivating method for prevention of disease and rice reinforced riboflavin - Google Patents

Abstract

The present invention relates to a method for cultivating rice and, more specifically, to a method for cultivating rice, wherein the rice is cultivated by spraying riboflavin, thereby significantly increasing the riboflavin content of the harvested rice and preventing disease in the rice, such as rice blast or bacterial blight, thus increasing productivity. The method for cultivating rice to allow disease control and produce rice enhanced with riboflavin is characterized in that riboflavin is sprayed to the rice when the rice is cultivated.

Description

[0001] The present invention relates to a rice plant cultivating method and a rice rib plant,

The present invention relates to a method of cultivating rice, and more particularly, to a method for cultivating rice by spraying riboflavin to increase the riboflavin content of harvested rice and to improve productivity by controlling rice blight such as blast disease or blight of blight The present invention relates to a method for growing rice.

Rice has been the ancestor of our ancestors and has formed the food culture of our country as the basis of our eating habits. In the past, when rice self-sufficiency was difficult, the increase of rice was an important task of agriculture, and efforts have been made to increase rice cultivation policies such as development of large variety varieties and development of cultivation techniques.

At the same time, in order to supplement deficient rice, wheat grain and marriage were strongly encouraged at once. Besides lack of nutritional research on rice in addition to seasoning exercise, wheat nutrition was once emphasized.

Therefore, the correct information on the nutrition and health of rice has not been provided, and misperception about rice has spread. Since then, rice has been self-sufficient since the 1980s due to the continuous promotion of rice and the development of breeding and cultivation technologies. Since then, the production of rice has continued to increase.

However, compared with the increasing supply of rice, the consumption of rice has been rapidly declining as our diet has been westernized recently. Therefore, the decrease in the consumption of rice caused the decrease of the rice price, and the farmers suffered a great difficulty. In addition, due to the considerable economic loss of domestic farmers and related companies due to the opening of the international rice market, the necessity of institutionalization for rice consumption measures, It is necessary to develop a new technology that can be differentiated.

In response to these demands, the development of functional rice has recently emerged. The emergence of such functional rice has changed the concept of rice as a stock from quantity to quality, .

In recent years, functional rice has been developed which has various flavors and nutrients in rice. For example, functional rice coated with ginseng, chitosan, germanium, mushroom, kelp, etc. has been developed recently . However, such conventional coated rice is obtained by coating functional materials on the surface of rice after harvesting, which is insufficient to meet consumers' preference in sensual taste such as taste and aroma, There is a problem that it is uneconomical because of expensive functional material.

Korean Patent Laid-Open Publication No. 2006-0015684 discloses a method of cultivating rice in which vitamins are increased so that infants in growing stage and vitamins that are likely to lack sufficient maternal nutrition during lactation can be consumed as food for rice.

The above-described conventional technique is not a method of coating rice, but a technique of increasing vitamin by folinic acid fertilization during the cultivation of rice, but it only increases the content of folic acid in harvested rice by about 30%. In addition, there is a problem in that the cultivation process is very complicated and practical.

On the other hand, the components of rice usually consist of 70 ~ 85% of carbohydrate, 6.5 ~ 8% of protein, 1 ~ 2% of fat, 22.5 ~ 5% of various minerals and 0.33mg of vitamin B1 per 100g of minerals, , vitamin B6 0.19mg, vitamin C 0.15mg, vitamin E 0.51mg, niacin 24.57mg, folic acid 40.30mg, pantothenic acid 2.07mg, magnesium 83.60mg, phosphorus 230.08mg, potassium 161.30mg, calcium 18.04mg.

Among the many nutrients in rice, riboflavin, called vitamin B2, is one of the important vitamins involved in various metabolisms. High fat, such as triglycerides and cholesterol, can cause arteriosclerosis. Deepening can cause myocardial infarction and stroke. At this time, vitamin B2 acts to reduce triglyceride and cholesterol. Another function of vitamin B2 is to prevent obesity by inhibiting the production of lipid peroxides. When Vitamin B2 is deficient, it can cause acne salt, keratitis, keratitis, seborrheic dermatitis, dry eye, eye irritation, cataract, and anemia.

The present invention has been made to overcome the above problems, and it is an object of the present invention to increase the content of riboflavin contained in rice by spraying riboflavin during the growing process of rice and to prevent the disease of rice such as blast or blight of blight, The purpose of this research is to provide a method for growing rice.

In order to accomplish the above object, the present invention provides a method for controlling disease and rice cultivation for producing riboflavin-enriched rice, wherein riboflavin is sprayed on rice and cultivated.

The application of the riboflavin is carried out at least once in the nutrition or growth and reproduction period of the rice.

Wherein the spraying is a leaf-surface spraying.

The disease is characterized by being a blast or a blight of blight.

As described above, according to the present invention, the content of riboflavin contained in the rice harvested can be greatly increased by spraying the rice with rice and cultivating the rice during the growing period.

In addition, riboflavin is sprayed on rice to increase the content of vitamins, and rice blight, such as blast or blight of blight, can be prevented, so that the productivity of rice can be improved by increasing the yield.

FIG. 1 is a graph showing the content of riboflavin contained in rice according to Example 1,
FIG. 2 is a graph showing the content of riboflavin contained in the stem of rice according to Experimental Example 2,
FIG. 3 is a graph showing the content of riboflavin contained in rice according to Experimental Example 3,
FIG. 4 is a graph showing a photograph of the seedlings according to the fourth experimental example,
FIG. 5 is a graph showing a photograph of a favorable rice according to the fourth experimental example,
6 is a photograph of rice according to the fourth experimental example,
FIG. 7 is a graph showing the bottle-growing distance of the rice seedlings according to the fifth experimental example,
FIG. 8 is a graph showing the bottle advance distance of a favorable rice according to the fifth experimental example,
9 is a graph showing a disease incidence rate according to the seventh experimental example,
10 is a graph showing the content of riboflavin contained in rice hulls and rice husks according to the seventh experimental example.

Hereinafter, a method for controlling disease according to a preferred embodiment of the present invention and cultivating rice for producing riboflavin-enriched rice will be described in detail with reference to the accompanying drawings.

In the rice cultivation method of the present invention, riboflavin is cultivated by spraying on rice. Riboflavin can be sprayed in diluted form in water. For example, 0.01 to 1.0 part by weight of riboflavin is added to 100 parts by weight of water and diluted. If the added amount of riboflavin is less than 0.01 part by weight, the effect is insignificant. If the added amount is more than 1.0 part by weight, the increase in the effect is lower than the added amount.

The amount of riboflavin added can be adjusted appropriately according to the time of spraying and the occurrence of the disease. In the case of spraying at the preventive level of disease, 0.1 part by weight of riboflavin is added to dilute in water, and 0.2 part by weight of riboflavin may be added to dilute in water when it is sprayed at the early stage of the disease.

The rice cultivation method of the present invention can be applied to both the conventional transplanting method and the direct seeding method. It is a method of cultivating rice seeds by germinating rice seeds in a mating area and transferring them to an answer in a certain manner. Most of the rice cultivation methods in this country are rice cultivation.

Direct seed cultivation is a method of seeding seeds directly into the seedlings without seedling seeds and allowing the seeds to grow until they are harvested. The seeds can be divided into dry seed cultivation method and direct water direct cultivation method according to water management.

Spraying of riboflavin occurs during the growth of rice in this answer. Riboflavin is preferably sprayed at least once on the nutritive or reproductive growth stages of the rice. The nutritive and reproductive growth periods of rice can be classified based on the bud formation time. The nutrient growth period of rice is a period in which rice grows vigorously in a year, which is also referred to as a period of growth (growth period), which means the period from the germination of rice to the formation of flower buds. The nutrient growth period can be divided into the seedling atmosphere, which is the period from germination to the time when the rice is germinated, and the tillering stage, where the flower bud is formed after the planting.

The term "reproductive growth period" refers to the period from the time when the flower bud is formed to the time when it blooms and disappears. The reproductive growth period is divided into the panicle formation stage, which is the period of the young spike, the booting stage, which is the egg-laying period for rice frying, and the grain filling stage, have.

The application of riboflavin is preferably applied to the nutrient growth or reproductive growth of rice plants. Riboflavin can be added to the irrigation water or sprayed directly onto the soil, but leaf spray is most effective. Foliar spraying is beneficial in reducing the changes in physico - chemical properties of soils and in absorbing riboflavin components in crops such as rice, which have a long growing period.

As described above, the content of riboflavin contained in the rice harvested can be greatly increased by spraying riboflavin on the nutrient growth or reproductive growth stage of rice grown in the present invention. The content of riboflavin contained in the rice cultivated and cultivated by a conventional method is 0.16 to 0.24 ppm, whereas the content of riboflavin in rice produced from the rice cultivated by the cultivation method of the present invention is as high as 0.3 to 0.8 ppm.

At the same time, the present invention is effective for controlling diseases caused in rice, especially blast or blight of white blossom by cultivating rice by spraying riboflavin. Therefore, the present invention has an advantage that the content of riboflavin of harvested rice is greatly increased, and rice paddy is prevented without using chemical pesticides to increase yield.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited by these examples.

<1. Riboflavin Content of Rice According to Number of Spreads>

 In - flight experiments were conducted to determine the optimal frequency of riboflavin use. A first sample (500X) in which 0.2 parts by weight of riboflavin was dissolved in 100 parts by weight of distilled water and a second sample (1000X) in which 0.1 parts by weight of riboflavin was dissolved in 100 parts by weight of distilled water and diluted 1000-fold was used Riboflavin content of rice was investigated according to the number of treatments.

During the growth period of rice, total 1 to 3 samples were sprayed on the leaf surface, and the final riboflavin content in rice grain was examined by harvesting at harvest time.

The experimental group consisted of a first group which was sprayed once in the tillering period of the nutrition and growth period and a second group which was sprayed once in the ripening period of the nutrition and growth period and twice in total, The seeds were divided into three groups: one spraying on the tillering stage and one spraying on the watering and maturing stages of the reproductive growth period, and spraying three times in total.

The experimental results are shown in Table 1 and FIG. 1, and the riboflavin content is expressed in ppm.

 division  One 2 3 Average
The first group
No treatment 0.207 0.221 0.198 0.208 ± 0.006 500 × 0.371 0.409 0.392 0.391 0.001 1000 × 0.393 0.392 0.312 0.365 + 0.026
The second group
No treatment 0.203 0.211 0.209 0.208 0.002 500 × 0.545 0.514 0.535 0.531 ± 0.009 1000 × 0.441 0.431 0.412 0.428 ± 0.008
Group 3
No treatment 0.211 0.221 0.234 0.222 ± 0.006 500 × 0.567 0.558 0.587 0.571 ± 0.008 1000 × 0.884 0.811 0.817 0.837 + 0.023

Referring to Table 1 and FIG. 1, in the case of the first group, about 0.2 ppm of riboflavin was detected in the untreated rice not sprayed with the sample (hereinafter referred to as the untreated rice), but the treated rice treated with the first sample diluted 500- (Hereinafter, referred to as a 500-fold dilution treatment) and a 1,000-fold diluted second sample (hereinafter referred to as a 1000-fold dilution) were detected as 0.39 ppm and 0.37 ppm, respectively. This is a result of about 1.9 times the difference between the control and non-treatment.

In the second group, the amount of riboflavin was about 2.9 times higher than that of untreated group at 0.53 ppm and 0.43 ppm at 500 and 1,000 dilution treatments, respectively.

In the third group, the difference between control and non - treatment was 0.22ppm and 0.57ppm and 0.84ppm, respectively.

As a result of the above test results, when riboflavin was sprayed onto rice, it was possible to obtain better results in terms of the content of riboflavin by spraying two or three times rather than only once, and about 4 times Of the total vitamin content.

<2. Riboflavin Content of Rice Stem According to Number of Sprays>

On the other hand, in order to determine the effect of riboflavin on the stem other than the grain in the case of cultivating rice, the stem content of the rice grown in the above experiment was sampled to investigate the vitamin content. The experimental results are shown in Table 2 and FIG.

 division  One 2 3 Average
The first group
No treatment 0.207 0.213 0.221 0.213 ± 0.004 500 × 1.021 0.984 1.029 1.011 + 0.013 1000 × 0.843 0.842 0.789 0.824 + 0.017
The second group
No treatment 0.188 0.201 0.199 0.196 + 0.041 500 × 0.895 0.987 0.996 0.959 + 0.032 1000 × 0.765 0.563 0.767 0.986 + 0.067
Group 3
No treatment 0.188 0.201 0.199 0.196 + 0.004 500 × 0.994 1.011 0.988 0.998 ± 0.006 1000 × 0.657 0.789 0.887 0.778 ± 0.006

Referring to Table 2 and FIG. 2, it was confirmed that riboflavin content was about 4 to 5 times higher in the first, second, and third groups in the 500-fold dilution and 1,000-fold dilution than the untreated group.

The results of this experiment showed that the vitamin content of the riboflavin was about 4 times higher than that of the untreated control, but the content of the vitamin was 4 times higher than that of the untreated control. Rice stem obtained as a by-product is expected to increase added value when it is used as feed for livestock because riboflavin content is high.

<3. Riboflavin Content of Rice According to Spraying Time>

 In order to determine optimal spraying time of riboflavin, experiments were carried out by dividing into nutritive and reproductive growth plants. A sample (500x) diluted 500 times and a second sample (1000x) diluted 1000x were used as in the above experiment.

The fourth group was harvested after spraying three times in 15 days intervals at intervals of 15 days. The fifth group harvested 3 times at 10 days intervals during the growing period of the reproductive growth period, The final contents of riboflavin in the wheat were examined, and rice varieties were favorably used.

The results of the experiment are shown in Table 3 and FIG. 3, and the riboflavin content is expressed in ppm.

 division  One 2 3 Average
Group 4
No treatment 0.207 0.221 0.198 0.208 ± 0.006 500 × 0.371 0.409 0.392 0.391 0.001 1000 × 0.393 0.392 0.312 0.365 + 0.026
Group 5
No treatment 0.211 0.221 0.234 0.222 ± 0.006 500 × 0.668 0.781 0.871 0.773 + - 0.058 1000 × 0.881 0.811 0.816 0.836 + 0.022

Referring to Table 3 and FIG. 3, in the fourth group, the untreated group contained about 0.2 ppm of riboflavin, whereas in the 500-fold and 1,000-fold diluted treatments, it increased to about 0.39 ppm and 0.37 ppm, respectively, about 1.6 to 1.8 times Could know.

In the 5th group, 0.25ppm of the untreated group, whereas the 500 and 1,000 dilution treatments of 0.77ppm and 0.84ppm, respectively, showed that the content of riboflavin was about 3.5 to 3.7 times higher.

Based on these results, it was confirmed that it is more effective to concentrate the reproductive growth period than the nutrition growing period to increase the content of riboflavin, and that the reproductive growth period is the optimum period for spraying the riboflavin.

<4. Experimental test of blast fungus>

Riboflavin produced vitamin - containing functional rice, and in - flight experiments were carried out to investigate the effect of controlling rice blast fungus.

Pyricularia causing blight ( Pyricularia oryzae strains were inoculated into PDA medium and cultured for one week at 25 ℃ incubator. Two rice varieties were used, which were susceptible to rice blast. One week after the rice was grown in the greenhouse for 3 months, the sample was sprayed once at the tillering stage of the nutrition and growth cycle. Pyricularia oryzae spores were prepared at 1 × 10 ⁵ spores / ml and sprayed so that rice could be sufficiently wetted. To prevent the leaves from drying immediately after spraying, rice seedlings inoculated with the strain were covered with vinyl for 2 days to increase the disease incidence. As in the above experiment, a first sample (500x) diluted with distilled water to 500x and a second sample (1000x) diluted to 1000x were used.

Two weeks after the inoculation of the pathogens, the percentage of lesion area in the blasted rice leaves was measured. The average lesion area ratio of the stem was examined as the percentage of diseased area per diseased area / number of irradiated specimens, and the ratio of the diseased areas with diseased areas was counted.

FIG. 4 is a graph showing the photographs of the rice seedlings after the experiment, and the graph showing the yield of the rice seedlings. FIG.

Referring to FIG. 4, in the case of the rice leaf cultivars, the blast ratio was about 40% at the 500-fold and 1,000-fold dilution compared to the control. The ratio of Lee, Byung - Joo according to the dilution factor was 40% level, respectively.

As shown in FIG. 5, the Hyeongpyong rice, which showed about 80% level of Leebyeongju in the non-treatment area, was found to be more vulnerable to blast disease than Ilyiri. And the 500 - fold and 1,000 - fold dilution treatment showed a 30% decrease in the number of the blastocysts compared to the control.

Table 4 below shows the results of the area ratio and control of the disease of the blast.

Percentage of lesion area (%) Control (%)
reputation
No treatment 69.0 + - 2.77 - 500 × 31.5 ± 3.34 54.35 1000 × 31.0 ± 3.79 55.07
Japan
No treatment 73.3 ± 1.67 - 500 × 40.0 + - 5.00 45.45 1000 × 38.3 + - 4.41 47.73

Referring to Table 4, it was confirmed that the area ratio of the lesion lesion was reduced by about 50% in both the US and the US. As compared with the 500 - and 1,000 - fold dilution, the percentage of lesion area as well as lesion area was twice as high in the untreated area, and the photograph of FIG. 6 showed that the untreated area did not grow taller due to the lesion.

<5. Experiment to control blight of blight of white blossom>

In - flight experiments were carried out to investigate the effect of controlling the blight of blight in rice cultivation. Xanthomonas causing blight of blight oryzae pv . Oryzae K3 race was cultured in NB medium. Culturing conditions were incubated for 48 hours at 28 ℃ shaking incubator (200 rpm).

One week after spraying the samples with rice grown in the greenhouse for 3 months, Xanthomonas oryzae pv . The Oryzae K3 race was inoculated with sterile water at OD (600 nm) = 1.0. As in the above experiment, the first sample (500 ×) and the second sample (1000 ×) diluted 1000 times were used, in which riboflavin was diluted 500 times with distilled water. The experiment was divided into six groups: one group was sprayed once during the ripening period of the nutritional genitalia, and the seventh group was sprayed three times at weekly intervals.

One week after the spraying, 15 pathogens were inoculated with scissors and 15 leaves per week. The distance of the disease progression was measured from the point cut by the scissors to the distance advancing to the lower section. Two varieties were used for the rice varieties.

Experimental results of the sixth group are shown in Table 5, Fig. 7, and Fig. FIG. 7 is a graph showing the bottle advance distance of the rice seedlings, and FIG. 8 is a graph showing the bottle advance distance of the popular rice.

Progression distance (cm) Control (%)
reputation
No treatment 17.4 ± 1.013 - 500 × 13.47 ± 0.930 22.61 1000 × 14.47 ± 1.081 16.82
Japan
No treatment 12.87 ± 1.280 - 500 × 8.27 ± 0.805 45.45 1000 × 8.33 + - 0.785 47.73

Referring to Table 5 and FIG. 7 and FIG. 8, in the 500-fold and 1,000-fold dilution treatments (bottle development distance: about 8 cm), the thermal conductivity of the rice was lower than that of the untreated rice (about 13 cm). The distances of disease progression were lower in 500 - fold and 1,000 - fold dilution treatments (bottle development distance: about 13cm ~ 14cm) compared to control treatment (disturbance distance: about 17.5cm) The 500 - fold and 1,000 - fold dilution treatments showed no significant difference between the 500 - fold and 1,000 - fold dilutions.

On the other hand, results for the seventh group were not shown, but similar results were obtained for the sixth group. Therefore, it can be confirmed that the treatment effect is only one treatment.

<6. Comparative analysis of components of rice>

In order to investigate the changes of the components and various other substances in comparison with the rice cultivated by the rice cultivation method of the present invention, general component analysis, heavy metal analysis, nutrient component analysis, constituent sugar analysis, amino acid analysis And fatty acid analysis.

Rice harvested by treating a first sample (500X) diluted with distilled water to 500 times of the third group of Experiment 1 was used as a test sphere. And rice cultivated and harvested without treatment with riboflavin as a control.

(1) General composition analysis

   The moisture content, protein content, amylose content and whiteness and the taste of rice contained in the rice were analyzed by the general composition analysis. The analysis was commissioned to the analysis institution of the NACF, and the results are shown in Table 6 below.

division Moisture (wt%) Protein (wt%) Amylose (wt%) Whiteness (%) Food (%) Control 14.3 6.9 15.8 40.6 57 Test section 14.3 6.9 15.7 40.3 59

Referring to Table 6, there was no significant difference in whiteness and graininess, including moisture content, protein content and amylose content in the test and control groups.

(2) Analysis of inorganic components

The pretreatment for inorganic component analysis was applied by wet decomposition method (Method 3051, EPA, USA) of US Environmental Protection Agency (EPA). (10 ml) of nitric acid for hazardous heavy metals (Junsei Chemical Co., Ltd., Tokyo, Japan) was added to the sample and 0.5 g of the powder sample was accurately weighed. The sample was digested with high performance microwave digestion unit (mls 1200 mega, milestone srl, USA) . After the decomposed samples were finally adjusted to 25 mL, the inorganic content was analyzed using ICP-OES (Inductively coupled plasma optical emission spectrometer, Optima 7000DV, Perkin-Elmer, CT, USA) The inorganic components were analyzed by heavy metals and nutrients. Heavy metals such as arsenic, lead, cadmium and mercury were analyzed. Iron, magnesium, zinc and calcium were analyzed as nutrients. The analytical results of inorganic components (ppm) are shown in Table 7 below.

division As Pb CD Hg Fe Mg Zn Ca Control 0.700 0.500 - - 39.400 1155.100 21.100 184,000 Test section 0.600 0.700 - - 70.400 1280,000 19.400 169.100

Referring to Table 7, it was confirmed that there were no significant differences in heavy metals and nutrients between the test group and the control group.

(3) analysis per constitution

The constituent sugars were tested by Gancedo et al., And 1 g of the powder sample was weighed, and 50 mL of 80% ethanol was added thereto. The mixture was refluxed for 2 hours at 80 ° C., filtered through a filter paper (Whatman No. 2) And the mixture was adjusted to 10 mL. After centrifugation (5,000 rpm, 15 min), the supernatant was collected and filtered through a 0.45 μm membrane filter (G3, Milipore, USA) and analyzed by ion chromatography (Dionex 600, Dionex, Germany). The results of the analysis (mg / L) per composition are shown in Table 8 below.

division Galactose Mannose Glucose Fructose Ribose Lactose Control 18.019 - - - 477.012 - Test section 19.396 - - - 463.793 -

Referring to Table 8, it was confirmed that there was no significant difference in the values per constitution of the test group and the control group even in the case of the constituent sugar.

(4) Analysis of amino acids

Amino acid analysis was performed by weighing 0.5 g of powder sample accurately in 18 mL test tube, adding 3 mL of 6N HCl and sealing the test tube with a vacuum pump. The sealed test tube was hydrolyzed for 24 hours in a heating block set at 121 ° C. The hydrolyzed samples were removed with a rotary evaporator (N-1000, Eyela, Tokyo, Japan) equipped with a cooling aspirator (Thermo-circulator, Daihan Labtech Co., Ltd., Korea) , And 1 mL of the solution was filtered with 0.2 uL of membrane filter and quantitatively analyzed with an amino acid analyzer (Pharmacia Biochrom 20, Li + type high performance ultra-pack, UK). The results of amino acid (mg / kg) analysis are shown in Table 9 below.

amino acid Control Test section Aspatic acid 611.151 622.056 Threonine 228.842 239.006 Serine 354.212 362.869 Glutamic acid 1225.308 1230.931 Proline 290.628 276.897 Glycine 302.246 301.115 Alanine 358.587 354.766 Cystine 137.314 127.819 Valine 384.060 390.286 Methionine 110.834 115.976 Isoleucine 224.446 241.242 Leucine 532.610 533.397 Tyrosine 75.716 90.688 Phenylalanine 342.987 343.301 Histidine 225.040 243.907 Lysine 245.581 252.386 Ammonia 530.315 537.810 Arginine 479.880 511.523 Total 6659.757 6775.975

Referring to Table 9, the content of amino acid components in the test group and the control group was not significantly different.

(5) Fatty acid analysis

For analysis of fatty acids, 5 g of sample is collected in a beaker and homogenized with a blander (Waring Products, Inc.). After adding 10 mL of chloroform and 20 mL of methanol, the mixture was homogenized again for about 2 minutes and injected with 10 mL of chloroform solution for about 30 seconds. Watman No. 1 filter paper and transfer the filtrate to a 50 ml measuring cylinder and wait for about 30 minutes until the layer is separated. After separating the two layers, remove the upper layer (water layer) and add Na ₂ CO ₄ reagent to the lower layer After removing the water completely, chloroform in the sample was completely removed by using a rotary evaporator. When the remaining fat component remained, it was dissolved in 5 ml of toluene and subjected to methylation analysis. The results are shown in Table 10 below.

Fattyacid Control Test section Butyric Acid (C4: 0) - - Caproic Acid (C6: 0) - - Caprylic Acid (C8: 0) - - Capric acid (C10: 0) - - Undecanoic acid (C11: 0) - - Lauric acid (C12: 0) - - Tridecanoic acid (C13: 0) - - Myristic acid (C14: 0) 0.54 0.56 Pentadecanoic acid (C15: 0) - - Palmitic acid (C16: 0) 19.69 19.76 Heptadecanoic acid (C17: 0) - - Stearic acid (C18: 0) 1.41 1.55 Arachidic acid (C20: 0) 0.33 0.38 Heneicosanoic acid (C21: 0) - - Behenic acid (C22: 0) - - Tricosanoic acid (C23: 0) - - Lignoceric acid (C24: 0) 0.43 0.47 Saturates 21.88 21.12 Myristoleic acid (C14: 1) - - cis-10-Pentadecenoic acid (C15: 1) - - Palmitoleic acid (C16: 1) - - cis-10-Heptadecenoic acid (C17: 1) - - Elaidic acid (C18: 1n9t) - - Oleic acid (C18: 1n9c) 30.66 33.67 cis-11-Eicosenoic acid (C20: 1) 0.32 0.00 Erucic acid (C22: 1n9) - - Nervonic acid (C24: 1) - - Monoenes 30.25 31.31 Linolelaidic acid (C18: 2n6t) Linoleic acid (C18: 2n6c) 47.12 49.58 cis-11,14-Eicosadienoic acid (C20: 2) - - cis-13,16-Docosadienoic acid (C22: 2) - - γ-Linolenic acid (C18: 3n6) - - Linolenic acid (C18: 3n3) 1.57 1.58 cis-8,11,14-Eicosatrienoic acid (C20: 3n6) - - cis-11,14,17-Eicosatienoic acid (C20: 3n3) 0.32 0.00 Arachidonic acid (C20: 4n6) - - cis-5,8,11,14,17-Eicosapentaenoic acid (C20: 5n3) - - cis-4,7,10,13,16,19-Docosahexaenoic acid (C22: 6n3) - - Polyenes 47.87 47.57

As shown in Table 10, the contents of fatty acid components in the test group and the control group were not significantly different from each other.

<7. Farmhouse Package Test>

In order to investigate the effect of rice blast prevention and the content of riboflavin in rice paddies, we tested the effect of the pesticide - free rice paddy in Daedong - myeon, Hampyeong - gun, Korea (growers: Chung Young - Joo and Kwak, Owon). Packing experiments were carried out from June 2011 to October 2011 and divided into treatments with no treatment and samples. The treatments were divided into two treatments: one treatment in which the sample was once treated at the tillering stage of the nutrition and growth stages, two treatments in which the treatments were carried out twice in total, Three treatments were carried out three times, one for each of the flowering period and the watering period of the reproductive organs, and four times for four times, one time each for the flowering period of the vegetative growth period, Respectively.

A first sample (500x) in which riboflavin was dissolved in distilled water and diluted 500-fold was used as a sample.

The disease incidence rate was measured according to the degree of disease in the leaves of the blast lesions occurred in each treatments, and the results of the incontinence control were examined. The results are shown in FIG. 9 and Table 11.

division Average disease incidence (%) Control (%) Non-treatment 26.8 - 1 time treatment 15.2 43.48 2 times treatment 10.5 60.87 3 times treatment 9.2 65.84 4 times treatment 10.7 60.25

Referring to Table 11 and FIG. 9, the disease incidence of the single treatments was about 15.2%, and the control value was about 43.48% as compared with the untreated ones. The incidence of disease in the 2 treatments was 10.5% and the control value was about 60.87% compared to the control. In addition, the treatments of 3 times and 4 times treatments showed more than 60% of control, compared with untreated treatments, but did not show significant results compared with 2 treatments. As a result, it is anticipated that more than 60% of blast fungus treatment will be effective when treated at least twice in actual packaging.

Then, the content of riboflavin was measured in rice hulls and white rice harvested from the 3-treatments and the untreated harvests, and the results are shown in FIG.

Referring to FIG. 10, the content of riboflavin was increased about 1.2 times as compared with that of the untreated rice, and the content of riboflavin was increased about 1.83 times as compared with the control untreated rice.

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (4)

A method for cultivating paddy rice for the production of riboflavin-enriched rice and a disease control system characterized in that rice is cultivated by spraying riboflavin on rice. [2] The method of claim 1, wherein the application of the riboflavin is performed at least once in the nutrition or reproduction / growth period of the rice, and the method of growing rice for producing the riboflavin-enriched rice. [Claim 3] The method according to claim 1, wherein the spraying is a foliar spraying, and a method for growing rice for producing riboflavin-enriched rice. [Claim 3] The method according to claim 1, wherein the disease is blast disease or blight of blight, and a method for growing rice for producing riboflavin-enriched rice.

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