CN104886089A - Application of active polysaccharides in corn top rot control - Google Patents
Application of active polysaccharides in corn top rot control Download PDFInfo
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- CN104886089A CN104886089A CN201510355685.6A CN201510355685A CN104886089A CN 104886089 A CN104886089 A CN 104886089A CN 201510355685 A CN201510355685 A CN 201510355685A CN 104886089 A CN104886089 A CN 104886089A
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Abstract
The invention relates to application of active polysaccharides in corn top rot control. Active polysaccharide 1.0-361ppm is used dependently foliar spray, combined with soluble salt containing plant nutrition in use or combined with pesticide in use, and is evident in corn top rot control effect. The active polysaccharide comprises chitosan oligosaccharide and glucosan and has evident resistance induction activity on crops, environmental stress resistance is enhanced by inducing basic resistance, and occurrence and development of corn top rot are prevented and controlled.
Description
Technical field
The present invention relates to the novelty teabag of active polysaccharide, particularly relate to the application of active polysaccharide in control of maize top rot.
Background technology
Corn top-rotten disease is corn new expression, can be divided into symptom bacillaryly to infect, fungal infections, the mixed infection of fungi bacterium.Corn top-rotten disease Symptoms complexity is various, can infect morbidity whole vegetative period at corn.Wheat seeding is that plant strain growth is slow, and yellow streak, appears in blade edge chlorosis, leaf malformation, shrinkage or distortion, and grave illness strain is withered or dead; The middle and late growth stage shows as leaf base and rots, only deposits master pulse, the complete but how deformity in middle and upper part, rots in rear raw young leaves top, causes that blade is short and small, blade tip is withered or incomplete, and blade edge often occurs cutting incising and yellow striped of shape like cutter; Strain shows as appearance and downgrades in various degree, and top vane is short and small, tissue is incomplete or gauffer distortion, and basal part of stem internode is short, often has channel-like cracking of seemingly damaging by worms, the visible brown stain of vertical section, and the slight disease plant later stage can take out male knot fringe, but how shaky female fringe is little.Susceptible strain root system is undeveloped, and main root is short, root hair is many and thin in velvet-like, and root cap rots brown stain.Within 1998, at Fuxin, Liaoning Province Late Cambrian, onset area accounts for 1/3 of maize sown area to corn top-rotten disease then, and very fast at China's developmenting spread in recent years, the state of an illness increases the weight of year by year, and occurrence scope constantly expands.The provinces and cities such as current Jilin, Liaoning, Heilungkiang, Xinjiang, Gansu, Ningxia, Hebei, Shandong, Henan, Beijing, the incidence of disease is 5%-34%, and plot, local is up to more than 70%.Corn top-rotten disease occurs extensively, and harm is serious, and morbidity strain is had no harvest substantially, and Field diseases is production loss rate, progressively becomes the important disease of corn.
The domestic and international research to corn top-rotten disease is less at present, lack systematic research and effectively preventing measure, pathogenesis is failed to understand, control difficulty, the control of maize top rot medicament carried out is bactericide, fungicide or the two mixing, carry out symptomatic treatment, all without obvious control efficiency, there is no the report of control of maize top rot Be very effective medicament.The present inventor passes through study of incident mechanism for many years, first corn top-rotten disease is classified as environment-stress venereal disease evil by pathogenicity disease at home, by induction basal resistance, improve diseased plant resistance against diseases and opposing environment-stress ability, slow down and eliminate the allergy that pathogen causes, facilitate pathogen symbiosis compatible with corn, the generation of prevention and corntrol corn top-rotten disease and development, be the new discovery of corn top-rotten disease study of incident mechanism and chemical control research, there is not yet relevant report at home and abroad.
Summary of the invention
The object of this invention is to provide the novelty teabag of active polysaccharide.
The invention provides the application of active polysaccharide in the pesticide control preparing corn top-rotten disease.
Above-mentioned application:
Described pesticide control contains active polysaccharide.
Preferably, described pesticide control is active polysaccharide, or the mixture of active polysaccharide and the soluble-salt containing plant nutrition, or the mixture of active polysaccharide and agricultural chemicals.
Described active polysaccharide is one or both of chitosan oligosaccharide or Portugal's polyene sugar.
Described active polysaccharide is preferably the mixture of Portugal's polyene sugar or chitosan oligosaccharide and Portugal's polyene sugar.
The described soluble-salt containing plant nutrition is the soluble inorganic salt containing plant macroelement, moderate-element, trace element or organic salt.
Of the present invention for mixed agricultural chemicals, be insecticide, bactericide, plant growth regulator.
Application of the present invention, the consumption of active polysaccharide is 1.0-361ppm, and using method is foliar spray.
The application of active polysaccharide provided by the invention has the following advantages:
1, active polysaccharide of the present invention is chitosan oligosaccharide or Portugal's polyene sugar, wherein:
Chitosan oligosaccharide (Oligosaccharins), chemical name β-Isosorbide-5-Nitrae-oligomerization-gucosamine, belonging to polysaccharose bioactivator, is a kind of plant resistance to environment stress derivant, soluble in water, stable in weak acid and weak base solution, and micro-poison, is easy to degraded in environment.Chitosan oligosaccharide can not only activate plant cell, short root strong sprout, Promoting plant growth, and can activated plant immune system, the activity that the raising defensive ferment relevant to disease-resistant and degeneration-resistant metabolism and Active oxygen eliminating enzyme system are united, the resistance such as the disease resistance of inducing plant, cold resistance, high temperature resistance, fighting drought, anti-saline and alkaline, anti-fertilizer damage, prevent, alleviate and repair the injury of adverse circumstance to plant cell.
Portugal's polyene sugar belongs to polysaccharose bioactivator, is a kind of novel plant resistance to environment stress derivant, and micro-poison is soluble in water.Portugal's polyene sugar can produce the protective plant protecting agent killing pathogenic microorganism by inducing plant as external source regulatory factor, reduces the generation of various crop disease; Can also as the effective Promoting plant growth of growth regulator, branch, bloom, every metabolic activity such as result, improve crop yield.
2, inventor is through corn top-rotten disease study of incident mechanism for many years, find that the immediate cause that corn top-rotten disease is fallen ill is bad environmental condition, for environment-stress venereal disease evil, the essence of aobvious disease is the systemic disease resistance that bad environmental condition reduces corn, induce pathologic disease, show as fungal infection, bacteriological infection or fungi bacteria mixed infection, effectively preventing approach should be induction of resistance.
3, compared with the prior art promoted, the corn top-rotten disease pesticide control carried out at present is fungicide and bactericide, and carry out symptomatic treatment, control efficiency is very poor; The present invention utilizes the induction of resistance effect of active polysaccharide, and induction basal resistance, improves disease resistance and resistance, and strengthen the ability resisting environment-stress, slow down corn allergy, the generation of prevention and corntrol corn top-rotten disease and development, control efficiency is remarkable.
4, the novelty teabag of active polysaccharide provided by the invention, using method is the common practise of this area, as foliar spray, fills with root, drip irrigation etc., preferred foliar spray.Not only can spray the generation of prevention corn top-rotten disease in advance, and can treat after corn top-rotten disease occurs.Those skilled in the art can adjust consumption as required.
Embodiment
Following examples and experimental example further illustrate content of the present invention, but should not be construed as limitation of the present invention.
In the following examples, the particle size range of all raw materials is not limit, and in order to meet the mobility of preparation, the needs of the uniformity, preferred size is the raw material components of 0.01-0.99mm, but should not be construed as limiting the invention.
In addition, the present invention's active polysaccharide used is the higher raw material of commercially available purity, and purity does not reach 100%, but can not produce other impact to control efficiency.
Embodiment 1 chitosan oligosaccharide
The chitosan oligosaccharide of 90% is diluted 4500 times of liquid-9 ten thousand times of liquid, be mixed with the solution that chitosan oligosaccharide concentration is 10-200ppm, foliage-spray control of maize top rot.
Embodiment 2 Portugal polyene sugar
95% Portugal's polyene malt sugar is released 20,000 times of liquid-95 ten thousand times of liquid, being mixed with Portugal's polyene sugar concentration is the solution of 1.0-47.5ppm, foliage-spray control of maize top rot.
Embodiment 3 chitosan oligosaccharides+Portugal's polyene sugar
Take 90% chitosan oligosaccharide 95.5g, 95% Portugal polyene sugar 4.5g, mix, the mixture preparation of the Viability polyoses content 90.2% (chitosan oligosaccharide and Portugal's polyene sugar ratio are 20:1) of mixture.Mixture is diluted 2500 times of liquid-5 ten thousand times of liquid, be mixed with the solution (chitosan oligosaccharide+Portugal's polyene sugar=20+1) that active polysaccharide concentration is 18.0-361ppm, foliage-spray control of maize top rot.
Embodiment 4 chitosan oligosaccharides+copper chloride+manganese chloride
Take 90% chitosan oligosaccharide 22.2g, copper chloride 38.9g, manganese chloride 38.9g, mix, mixture becomes the mixture preparation of chitosan oligosaccharide content 20%.Mixture is diluted 1000 times of liquid-2 ten thousand times of liquid, be mixed with the solution that chitosan oligosaccharide concentration is 10-200ppm, foliage-spray control of maize top rot.
Embodiment 5 Portugal polyene sugar+calcium acetate
Take 95% Portugal polyene sugar 10.5g, calcium acetate 89.5g, mix, mixture becomes the mixture preparation of Portugal's polyene sugar content 10%.Mixture is diluted 2000 times of liquid-10 ten thousand times of liquid, being mixed with Portugal's polyene sugar concentration is the solution of 1-50ppm, foliage-spray control of maize top rot.
Embodiment 6 chitosan oligosaccharides+potassium dihydrogen phosphate+potassium sulfate
Take 90% chitosan oligosaccharide 11.1g, potassium dihydrogen phosphate 40.0g, potassium sulfate 48.9g, mix, mixture becomes the mixture preparation of chitosan oligosaccharide content 10%.Mixture is diluted 500 times of liquid-1 ten thousand times of liquid, be mixed with the solution that chitosan oligosaccharide concentration is 10-200ppm, foliage-spray control of maize top rot.
Embodiment 7 Portugal polyene sugar+carbendazim
Take 95% Portugal polyene sugar 10.5g, 50% carbendazol wettable powder 89.5g, mix, mixture becomes the mixture preparation of Portugal's polyene sugar content 10%.Mixture is diluted 2000 times of liquid-10 ten thousand times of liquid, being mixed with Portugal's polyene sugar concentration is the solution of 1-50ppm, foliage-spray control of maize top rot.
Embodiment 8 chitosan oligosaccharides+Imidacloprid
Take 90% chitosan oligosaccharide 11.1g, 20% imidacloprid soluble powder agent 88.9g, mix, mixture becomes the mixture preparation of Portugal's polyene sugar content 10%.Mixture is diluted 500 times of liquid-1 ten thousand times of liquid, be mixed with the solution that chitosan oligosaccharide concentration is 10-200ppm, foliage-spray control of maize top rot.
Experimental example 1
1 test method
Test process and spraying concentration are in table 1.Wherein, process 1-8 is respectively solution prepared by embodiment 1-8; Process 9 is medicament contrast " the solvable pulvis of 50% carbendazol wettable powder+72% agricultural streptomycin " (1+1) ck
1; Process 10 is clear water contrast ck
0.
Experimental field select at Qingfeng County willow lattice.Corn variety is for dredging single 20, and density 4500 plants/acre, waits line-spacing to plant, line-spacing 0.6 meter.Corn top-rotten disease initial stage of origination, each marks for treatment diseased plant 3 groups, often organizes 100 strains, within 3 days, sprays once, sprays 2 times altogether.Second time sprays 4 days " Invest, Then Investigate " incidences.
Grade scale: healthy tree, normal strain or diseased plant restore normal growth; Diseased plant, display diseased plant symptom, does not recover or does not restore normal growth completely.
Control efficiency computing formula is as follows:
Diseased plant rate (%)=diseased plant number/investigate total strain number × 100
Control efficiency (%)=(1-process diseased plant rate/contrast diseased plant rate) × 100
Process tested by table 1
2 result of the tests: in table 2.
Table 2 control of maize top rot result of the test
Table 2 result shows:
1) effect processing 1-8 control of maize top rot is 53.3%-78.7%, is all extremely significantly better than the preventive effect 41.0% of bactericidal agent contrast.Show that active polysaccharide and mixture preparation thereof can be effective to control of maize top rot.
2) process the control efficiency 71.3% of 3, be significantly better than the control efficiency 53.3%, 62.0% of process 1, process 2.Process 3 is the mixture of active polysaccharide chitosan oligosaccharide and Portugal's polyene sugar, active ingredient spraying concentration is respectively 71.5ppm and 3.5ppm, and the active ingredient spraying concentration of its correspondence is all lower than the active ingredient spraying concentration 100ppm of process 1, the active ingredient spraying concentration 5ppm of process 2.Show that chitosan oligosaccharide and the coupling of Portugal's polyene sugar have synergy.
3) processing 4, processing the control efficiency of 5 is 77.7%, 78.7%, is significantly better than chitosan oligosaccharide single dose process 1, Portugal's polyene sugar single dose process 2.Process 4, process 5 are respectively chitosan oligosaccharide, Portugal's polyene sugar and moderate-element, micro-mixture.Result shows, chitosan oligosaccharide, Portugal's polyene sugar and moderate-element, has synergy between trace element.
4) process the mixture that 6,7,8 are respectively active polysaccharide and macroelement, insecticide, bactericide, control efficiency and corresponding active polysaccharide single dose are without significant difference.Result shows, macroelement, insecticide, bactericide without synergistic effect, but can meet other demand of farming to active polysaccharide.
Experimental example 2
1 test process
Process 1-4 is respectively the solvable pulvis of 0.5% Portugal's polyene sugar, 5% chitosan oligosaccharide aqua, 6% oligosaccharide element aqua, 2% lentinan aqua, commercially available product; Process 5 is clear water contrast ck
0.
2 test methods
Test site, method are with experimental example 1.
3 result of the tests: in table 3.
Table 3 polysaccharose control of maize top rot comparative trial
Table 3 result shows, the effect of process 1-4 Portugal polyene sugar 5ppm, chitosan oligosaccharide 100ppm, oligosaccharide element 100ppm, lentinan 100ppm control of maize top rot is respectively 63.3%, 55.3%, 46.0%, 44.0%, the preventive effect of Portugal's polyene sugar is best, is significantly better than chitosan oligosaccharide, oligosaccharide element, lentinan; The preventive effect of chitosan oligosaccharide is significantly better than oligosaccharide element, lentinan; Preventive effect and the lentinan of oligosaccharide element are suitable.
Although above with general explanation, embodiment and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (7)
1. the application of active polysaccharide in the pesticide control preparing corn top-rotten disease.
2. application according to claim 1, is characterized in that, described pesticide control contains active polysaccharide.
3. application according to claim 2, described pesticide control is active polysaccharide, or the mixture of active polysaccharide and the soluble-salt containing plant nutrition, or the mixture of active polysaccharide and agricultural chemicals.
4. the application according to any one of claim 1-3, is characterized in that, described active polysaccharide is one or both of chitosan oligosaccharide or Portugal's polyene sugar.
5. application according to claim 3, is characterized in that the described soluble-salt containing plant nutrition, is the soluble inorganic salt containing plant macroelement, moderate-element, trace element or organic salt.
6. application according to claim 3, is characterized in that, described agricultural chemicals is insecticide, bactericide or plant growth regulator.
7. the application according to any one of claim 1-6, is characterized in that, using method is active polysaccharide 1.0-361ppm foliar spray.
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Cited By (6)
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| CN106719665A (en) * | 2016-11-21 | 2017-05-31 | 许卫峰 | A kind of bactericide of the sugar of polyene containing Portugal and Xinjunan acetate |
| CN109704889A (en) * | 2018-12-29 | 2019-05-03 | 沃稞生物科技(大连)有限公司 | A kind of fertilizer synergistic auxiliary agent that root fertilizer is synchronous |
| CN109956813A (en) * | 2017-12-25 | 2019-07-02 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer and preparation method thereof improving fruit corn fruit quality |
| CN109956801A (en) * | 2017-12-25 | 2019-07-02 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer and preparation method thereof for fruit corn flower bud development |
| CN110105133A (en) * | 2019-04-28 | 2019-08-09 | 湖北工程学院 | A kind of dedicated degeneration-resistant foliar fertilizer of Brassica rapa L and its application method |
| CN113040148A (en) * | 2021-03-23 | 2021-06-29 | 四川百事东旺生物科技有限公司 | Agricultural composition containing glucan |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106719665A (en) * | 2016-11-21 | 2017-05-31 | 许卫峰 | A kind of bactericide of the sugar of polyene containing Portugal and Xinjunan acetate |
| CN109956813A (en) * | 2017-12-25 | 2019-07-02 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer and preparation method thereof improving fruit corn fruit quality |
| CN109956801A (en) * | 2017-12-25 | 2019-07-02 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer and preparation method thereof for fruit corn flower bud development |
| CN109704889A (en) * | 2018-12-29 | 2019-05-03 | 沃稞生物科技(大连)有限公司 | A kind of fertilizer synergistic auxiliary agent that root fertilizer is synchronous |
| CN110105133A (en) * | 2019-04-28 | 2019-08-09 | 湖北工程学院 | A kind of dedicated degeneration-resistant foliar fertilizer of Brassica rapa L and its application method |
| CN113040148A (en) * | 2021-03-23 | 2021-06-29 | 四川百事东旺生物科技有限公司 | Agricultural composition containing glucan |
| CN113040148B (en) * | 2021-03-23 | 2022-03-25 | 四川百事东旺生物科技有限公司 | Agricultural composition containing glucan |
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Inventor after: Guo Hongfu Inventor after: Liu Yaping Inventor after: Yue Zhenguo Inventor after: Gao Hongze Inventor after: Sun Gangqiang Inventor after: Wang Liumei Inventor after: Xue Zhongli Inventor after: Feng Zili Inventor after: Wang Xiangping Inventor after: Liu Hongjun Inventor after: Hu Yuxiang Inventor after: Wang Min Inventor after: Chen Luting Inventor after: Liu Xiaofan Inventor after: Guo Hui Inventor after: Zhou Juanli Inventor after: Zhao Ping Inventor after: Qi Xiaoguang Inventor after: Zhang Xiaoying Inventor after: Liang Bing Inventor after: Su Dongsheng Inventor after: Li Na Inventor after: Zhu Heqin Inventor after: Han Bing Inventor after: Jia Xinghua Inventor after: Wang Suna Inventor after: Wang Zhaoliang Inventor after: Kang Hailin Inventor after: Tang Huijuan Inventor after: Zhang Shujie Inventor after: Guo Hongfu Wang Min Zhu Heqin Wang Qinglian Li Zhengdao Cao Yunhui Sun Huatian Qin Haiying Liu Yaping Yue Zhenguo Gao Hongzesun Ren party should be Wang Liumei Xue Zhongli Feng Zili Wang Xiangping Liu Hongjun Hu Yuxiang and Chen Luting Liu Xiaofan Guo Hui Zhou Juanli Zhang Xiaoying Liang Bing Su Dongsheng Qi Ping Zhao Xiaoguang Li Na Han Bingjia Wang Suna Wang Zhaoliang Kang Hailin Tang Huijuan Zhang Shujie Wang Yating Xinghua Inventor after: Wang Qinglian Inventor after: Li Zhengdao Inventor after: Cao Junhui Inventor after: Sun Huatian Inventor after: Ren Yingdang Inventor after: Qin Haiying Inventor before: Guo Hongfu Inventor before: Xue Zhongli Inventor before: Feng Zili Inventor before: Wang Xiangping Inventor before: Liu Hongjun Inventor before: Hu Yuxiang Inventor before: Chen Luting Inventor before: Liu Xiaofan Inventor before: Guo Hui Inventor before: Zhou Juanli Inventor before: Zhao Ping Inventor before: Zhu Heqin Inventor before: Zhang Xiaoying Inventor before: Liang Bing Inventor before: Li Zhengdao Inventor before: Cao Junhui Inventor before: Sun Huatian Inventor before: Ren Yingdang Inventor before: Gao Hongze Inventor before: Sun Gangqiang Inventor before: Wang Liumei |
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