CN107801723A - Prevent and treat composition pesticide and its application of the viroses of plant - Google Patents
Prevent and treat composition pesticide and its application of the viroses of plant Download PDFInfo
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- CN107801723A CN107801723A CN201710916751.1A CN201710916751A CN107801723A CN 107801723 A CN107801723 A CN 107801723A CN 201710916751 A CN201710916751 A CN 201710916751A CN 107801723 A CN107801723 A CN 107801723A
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- plant
- viroses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to composition pesticide, specifically provides a kind of composition pesticide for preventing and treating the viroses of plant and its application.The composition pesticide of the present invention preventing and treating viroses of plant, its active component is Physcion and the β carboline class compounds containing acylhydrazone and bishydrazide structure, and each component is environmentally friendly, will not cause environmental pollution；It is good to Control of Plant Virus Disease effect meanwhile Physcion and the β carboline class compounds containing acylhydrazone and bishydrazide structure have synergistic function, it can induce and strengthen via plant immunity, so as to suppress infecting for virus, have the function that antiviral and increase production.
Description
Technical field
The present invention relates to pesticide field, and in particular to prevents and treats composition pesticide and its application of the viroses of plant.
Background technology
The viroses of plant are by the parasitogenic disease of plant virus.Plant virus must be sought parasitic raw in host cell
Living, specialization is strong, and a certain virus can only infect a certain or certain plants.The common extensive viroses of plant of harm have tobacco
Mosaic virus and cucumber mosaic virus.
General plant virus is only in vivo just active in host；Only a small number of plant viruses can protect in diseased plant residuum
Active several days, some months, even several years are held, also there are a small number of plant viruses in vivo can survive or breed in insect.Plant virus
The duplication of nucleic acid (RNA or DNA) and protein coat is carried out in host cell, forms new virion.Phytopathy virion
Body or viral nucleic acid transfer velocity between plant cell are very slow, and then can be fast with the nutrition flow direction of plant in vascular bundle
Speed transfer, the plant whole body is set to fall ill.
The Chinese patent application of Application No. 201310322255.5 discloses a kind of special effect agent for preventing and treating plant virus
And preparation method thereof, and specifically disclose the agent for prevention and control of virus and be made up of Moroxydine Hydrochloride, cantharidin, ammonium sulfate and DA-6,
But its component is more complicated, cost is also higher.
The Chinese patent application of Application No. 201310733788.2 discloses a kind of biological source for preventing and treating the viroses of plant
Composition pesticide and its application, the active component of the biogenic pesticide composition are made up of chitosan and Physcion,
But its preventive effect is not satisfactory, only up to 82.5%.
Therefore, the composition pesticide for finding the preventing and treating viroses of plant that component is simple, cost is cheap, preventive effect is excellent seems outstanding
To be important.
The content of the invention
To solve above technical problem, the invention provides a kind of composition pesticide for preventing and treating the viroses of plant and its answer
With.The composition pesticide of the preventing and treating viroses of plant of the present invention, its active component is Physcion and compound A, its
In, wherein compound A is (1S, 3S)-N '-(4- chlorobenzenes methylene) -1- methyl -2,3, and 4,9- tetrahydropyridines are simultaneously [3,4-b]
Indoles -3- formylhydrazines, (1S, 3S)-N '-(cyclohexylmethylene) -1- methyl -2,3,4,9- tetrahydropyridines simultaneously [3,4-b] indoles -
One or both of 3- formylhydrazines, structural formula is as follows：
(1S, 3S)-N '-(4- chlorobenzenes methylene) -1- methyl -2,3,4,9- tetrahydropyridines simultaneously [3,4-b] indoles -3- first
Hydrazides：
(1S, 3S)-N '-(cyclohexylmethylene) -1- methyl -2,3,4,9- tetrahydropyridines simultaneously [3,4-b] indoles -3- formyls
Hydrazine：
By weight, Physcion:Compound A=1~100:0.1~500；Preferably, Physcion:Chemical combination
Thing A=1~50:1~100；More preferably, Physcion:Compound A=1~10:1~50；The preventing and treating viroses of plant
Composition pesticide in the content of active component be 0.1~50wt%.
Wherein, compound A is the B-carboline class compound containing acylhydrazone and bishydrazide structure, to the resistance of tobacco mosaic virus (TMV)
Significantly larger than virazole, and have higher inhibitory action to apple wheel line, withered, the rape sclerotium of wheat line etc..
Physcion is protective fungicide, and induction crop produces defense reaction, suppresses pathogen spore germination, mycelia
Growth, the formation of haustorium so that infringement of the crop from pathogen.Both can produce synergistic function at combination.
In order to improve the activity of composition pesticide of the present invention, while it is easy to store and transports, the preventing and treating viroses of plant
Composition pesticide can add suitable auxiliary agent and be prepared into conventional any formulations of pesticide, these compositions can be according to anti-plant
The dosage for the dosage form selection routine that virus composition is prepared, this is known to those skilled in the art, will not be repeated here.
Further, the composition pesticide preferred dosage form of the present invention preventing and treating viroses of plant is aqueous emulsion or aqua or solvable
Property pulvis or missible oil or microemulsion or suspending agent or or wettable powder or water dispersible granules.
Preferably, the composition pesticide of the preventing and treating viroses of plant：
Aqueous emulsion composition is as follows：By weight, Physcion：5 parts, compound A：10 parts, agriculture breast 500#：2.8 parts,
Agriculture breast 602#：7.2 parts, ethylene glycol：20 parts, water：55 parts；
Soluble powder composition is as follows：By weight, Physcion：5 parts, compound A；0.1 part, lignin sulfonic acid
Sodium：1 part, lauryl sodium sulfate：1 part, potassium nitrate：92.9 parts；
Aqua composition is as follows：By weight, Physcion：0.1 part, compound A：10 parts, DBSA
Sodium：2 parts, OPEO：1 part, ethylene glycol：20 parts, water：66.9 parts.
Further, the invention also discloses the composition pesticide of the preventing and treating viroses of plant in the preventing and treating viroses of plant
Application：The virus includes tobacco mosaic virus (TMV) (TMV), TOMV (TOMV), cucumber mosaic virus (CMV), horse
Bell potato X viruses (PVX).
Application method：Preferably, in use, it is 10~100ppm to be diluted with water to active component concentration, preferably
For 50ppm.In use, composition pesticide of the present invention is sprayed in plant blade face, to occur water membrane on blade face, decoction not under
Drop is advisable；Carry out secondary dispenser after being preferable over 7~10 days, one season the preferred continuous administration of crop 2~3 times.
Compared with prior art, the present invention has advantages below：
1st, the composition pesticide of the present invention preventing and treating viroses of plant is used as activity using Physcion and compound A compoundings
Component, each component is environmentally friendly, will not cause environmental pollution.
2nd, Physcion and compound A have synergistic function in composition pesticide of the present invention, to the viroses of plant
Prevention effect is good, can induce and strengthens via plant immunity, so as to suppress infecting for virus, has the function that antiviral and increases production.
3rd, the composition pesticide of the present invention preventing and treating viroses of plant can be prepared into aqua, soluble powder or wettable powder
It is flexible and convenient to use Deng regular dosage form, formulation variation.
4th, the composition pesticide of the present invention preventing and treating viroses of plant, which has concurrently, promotes growth, the effect of prevention and treatment virosis
Fruit, it is cost-effective, increase significant plant medical agent, preparation method is simple, and raw material is easy to get, and has wide market prospects.
Embodiment
The embodiment of the present invention is further described with reference to embodiment, therefore not limited the present invention
System is among described scope of embodiments.All technologies realized based on the above of the present invention belong to the scope of the present invention,
Unless otherwise specified, completed in following embodiments using conventional prior.
Embodiment
The preparation of the composition pesticide of the viroses of plant is prevented and treated, each component is weighed according to table 1, is mixed, it is anti-to produce the present invention
Control the composition pesticide of the viroses of plant.
Table 1：
Test example 1
Composition pesticide of the present invention is tested for Tomato mosaic virus
1 test material
1.1 for studying thing and object：Tomato, tobacco mosaic virus (TMV) (TMV)
1.2 medicament
Test medicine：Prepared composition pesticide SY-1~SY-8 in embodiment；Physcion single dose (D1)；Chemical combination
Thing A single doses (D2)；Clear water compares (CK)；
Wherein, the preparation method of Physcion single dose (D1) is：Take 5Kg Physcions, sodium lignin sulfonate 1kg,
Lauryl sodium sulfate 1kg, mix, complement to 100kg with potassium nitrate, soluble powder is made.
The preparation method of compound A single doses (D2) is：5Kg compound A are taken, add agriculture breast 500#2.8kg, agriculture breast 602#
7.2kg, ethylene glycol 20kg, add water to mend to 100kg, aqueous emulsion is made.
In use, it is 50ppm that above-mentioned medicament is diluted into active component concentration.
2 experimental designs and method
Test method is with reference to NYT1464.8-2007《The part of pesticide field efficacy medicine test criterion the 8th：Bactericidal agent for preventing and treating tomato
Virosis》.
Tomato virus disease：It is that the diseased plant as caused by tobacco mosaic virus (TMV) is in system floral leaf, sick leaf is less than normal, slightly Zou contracts；Diseased plant
Stem, branch, petiole, carpopodium even there is long and short unsuitable brown necrosis streak on vein, a large amount of fallen leaves, when serious only
Remain a small number of blades.There are yellow spot or necrotic plaque in disease fruit surface, and pulp is thinning, the tomato virus disease for the symptom such as come off ahead of time.
Disease investigation：
Control time：Carry out within 10 days after 2nd dispenser.
State of an illness partition of the level：
0 grade：It is asymptomatic；
1 grade：The vein of lobus cardiacus is bright arteries and veins, or acute small withered spot occurs in inoculation blade；
3 grades：A small number of light floral leaves of blade, or inoculation leaf abscission, stem produce small necrotic spot；
5 grades：Most blade floral leaves, a small number of leaf malformations, shrinkage, or stem produce downright bad streak；
7 grades：Most blades are attached most importance to floral leaf, deformity, shrinkage, and plant is downgraded, or stem, branch and vein have system necrotic plaque；
9 grades：Most blades are attached most importance to floral leaf, deformity, shrinkage, and plant is downgraded obvious；Or plant serious systemic necrosis.
Investigation method：
Every part of expert evidence inoculation strain incidence is investigated, is described according to Disease symptoms, material is investigated portionwise, is recorded
State of an illness rank, disease index PT is calculated according to formula 1：
PT=[∑ (Ni*i)/(N*9)] * 100 (1)
In formula：
PT-disease index；
Ni-sick the number of sheets at different levels；
I-relative value of series；
N-investigation total number of sheets.
If having carried out the investigation of state of an illness radix before dispenser, prevention effect is calculated by formula 2：
P=[1- (CK0/CK1)*(PT1/PT0)]*100········(2)
P-prevention effect (%)；
CK0Disease index before the dispenser of-blank district；
CK1Disease index after the dispenser of-blank district；
PT0Disease index before the dispenser of-chemicals treatment district；
PT1Disease index after the dispenser of-chemicals treatment district；
If not carrying out state of an illness radix investigation before dispenser, prevention effect is calculated by formula 3：
P=(1-PT1/PT0)*100·······(3)
Virosis is inoculated with the last week, and dispenser once, is inoculated with latter week, repeats dispenser once, and common dispenser is twice；Each test group
Formulation rate and disease index result see the table below：
Table 2：
| Treatment agent | Active component concentration (ppm) | 10 days preventive effects (%) after second of dispenser |
| SY-1 | 50 | 86.84 |
| SY-2 | 50 | 84.57 |
| SY-3 | 50 | 89.74 |
| SY-4 | 50 | 93.30 |
| SY-5 | 50 | 87.56 |
| SY-6 | 50 | 74.25 |
| SY-7 | 50 | 78.46 |
| SY-8 | 50 | 85.33 |
| D1 | 50 | 60.21 |
| D2 | 50 | 71.90 |
| CK | —— | —— |
As can be seen from the above table, there is higher preventive effect to tobacco mosaic virus (TMV) using composition pesticide of the present invention, wherein,
SY-4 pharmacy effects are best, and preventive effect has reached 93.3% after secondary medicine.Meanwhile compared with D1, D2 single dose, its preventive effect substantially carries
It is high, it is seen then that composition pesticide of the present invention has synergistic function.
Test example 2
Pharmaceutical composition of the present invention is used for the efficiency test of cucumber virosis
1 test material
1.1 for studying thing and object：Cucumber cucumber mosaic virus (CMV)
1.2 medicament
Using with identical medicament in test example 1.
2nd, experimental design and method
Test method is with reference to GB/T 17980.26-2000《Field control effectiveness test criterion (one)：Bactericidal agent for preventing and treating cucumber cream
Mildew》.
8 medicaments of this experiment, 2 single dose controls, 1 blank control, four repetitions, totally 32 supply examination cell.Minizone
And surrounding leaves isolation strip, plot area 30m2, cell respectively handles random alignment.
With reference to the investigation method of test example 1, show that each test group formulation rate and disease index result see the table below：
Table 3：
| Treatment agent | Active component concentration (ppm) | 10 days preventive effects (%) after second of dispenser |
| SY-1 | 50 | 83.77 |
| SY-2 | 50 | 87.30 |
| SY-3 | 50 | 91.18 |
| SY-4 | 50 | 94.01 |
| SY-5 | 50 | 92.53 |
| SY-6 | 50 | 80.26 |
| SY-7 | 50 | 93.98 |
| SY-8 | 50 | 79.20 |
| D1 | 50 | 55.85 |
| D2 | 50 | 67.55 |
| CK | —— | —— |
From table 3 it is observed that there is higher preventive effect to cucumber virosis using composition pesticide of the present invention, wherein, SY-
4 pharmacy effects are best, and preventive effect has reached 94.01%.Meanwhile compared with D1, D2 single dose, its preventive effect significantly improves, it is seen then that this hair
Bright composition pesticide has synergistic function.
Claims (7)
1. prevent and treat the composition pesticide of the viroses of plant, it is characterised in that：Its active component is Physcion and compound A,
Wherein, by weight, Physcion:Compound A=1~100:0.1~500；The content of active component be 0.1~
50wt%；
Wherein, compound A is (1S, 3S)-N '-(4- chlorobenzenes methylene) -1- methyl -2,3,4,9- tetrahydropyridines simultaneously [3,4-
B] indoles -3- formylhydrazines, (1S, 3S)-N '-(cyclohexylmethylene) -1- methyl -2,3,4,9- tetrahydropyridines simultaneously [3,4-b] Yin
One or both of diindyl -3- formylhydrazines.
2. the composition pesticide of the preventing and treating viroses of plant according to claim 1, it is characterised in that：In its active component,
By weight, Physcion:Compound A=1~50:1~100.
3. the composition pesticide of the preventing and treating viroses of plant according to claim 2, it is characterised in that：Its active component, is pressed
Weight ratio, Physcion:Compound A=1~10:1~50.
4. the composition pesticide of the preventing and treating viroses of plant according to claim 1, it is characterised in that：Described pesticide combination
Thing is aqueous emulsion or aqua or soluble powder or missible oil or microemulsion or suspending agent or wettable powder or water dispersible granules.
5. the application of the composition pesticide of the preventing and treating viroses of plant described in claim 1, it is characterised in that the phytopathy of preventing and treating
Poison includes tobacco mosaic virus (TMV), TOMV, cucumber mosaic virus, potato virus X.
6. the application of the composition pesticide of the preventing and treating viroses of plant according to claim 5, it is characterised in that：In use,
It is 10~100ppm to be diluted with water to active component concentration.
7. the application of the composition pesticide of the preventing and treating viroses of plant according to claim 6, it is characterised in that：In use,
It is 50ppm to be diluted with water to active component concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710916751.1A CN107801723A (en) | 2017-09-30 | 2017-09-30 | Prevent and treat composition pesticide and its application of the viroses of plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710916751.1A CN107801723A (en) | 2017-09-30 | 2017-09-30 | Prevent and treat composition pesticide and its application of the viroses of plant |
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| Publication Number | Publication Date |
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| CN107801723A true CN107801723A (en) | 2018-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710916751.1A Pending CN107801723A (en) | 2017-09-30 | 2017-09-30 | Prevent and treat composition pesticide and its application of the viroses of plant |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104744460A (en) * | 2013-12-30 | 2015-07-01 | 南开大学 | B-carboline, dihydro-B-carboline and tetrahydro-B-carboline alkaloid derivative as well as preparation method and application in aspects of plant virus prevention and cure, sterilization and insecticide |
-
2017
- 2017-09-30 CN CN201710916751.1A patent/CN107801723A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104744460A (en) * | 2013-12-30 | 2015-07-01 | 南开大学 | B-carboline, dihydro-B-carboline and tetrahydro-B-carboline alkaloid derivative as well as preparation method and application in aspects of plant virus prevention and cure, sterilization and insecticide |
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Application publication date: 20180316 |