CN104621147A - Tetramycin compound and application - Google Patents
Tetramycin compound and application Download PDFInfo
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- CN104621147A CN104621147A CN201510064126.XA CN201510064126A CN104621147A CN 104621147 A CN104621147 A CN 104621147A CN 201510064126 A CN201510064126 A CN 201510064126A CN 104621147 A CN104621147 A CN 104621147A
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Abstract
The invention relates to the fields of search and development of biopesticide preparations, and specifically relates to a tetramycin compound and an application. The invention is the divisional application applied on 26th, November, 2013 with the application number of 2013106143470, and is named tetramycin compound and an application. The tetramycin compound is prepared from tetramycin and active substances, wherein the active substances are esters, including one or a plurality of the following substances: dithiocarbamate, carbamate and pyrethroid. Compared with the prior art, the tetramycin compound and an application have the advantages that the danger of resistant fungi strain selection is reduced, the service lives of the active components are prolonged, and the prevention and control scope is expanded; the disease preventing effect is improved; the drug resistance of bacteria can be delayed or overcome; the cost is saved; and the times of application of pesticides can be reduced.
Description
The divisional application that the present patent application is application number is 2013106143470, the applying date is on November 26th, 2013, denomination of invention is " tetramycin complex and application ".
Technical field
The present invention relates to biopesticide preparaton research and development field, in particular to tetramycin complex and application.
Background technology
At present, the pesticide species for plant protection is various, and mostly pesticide species is meddlesome, but also brings a lot of problem.Mostly agricultural chemicals is that a worm one is controlled, a medicine one is controlled, thus agricultural chemicals can only be prevented and treated for a kind of insect pest or disease, does not produce effect, even cause the accident of the underproduction to other murder by poisoning or disease, and replay in field, No kernels or seeds are gathered, as in a year of scarcity for even ruining of having.The plant protection technology that a this worm one is controlled, a medicine one is controlled is not suitable with the needs of agricultural development, and peasant controls in the urgent need to a medicine more, sick worm is doublely controlled, economize medicine saving of labor, protect the dispenser new technology of producing volume increase, easy to use, remarkable benefit.
Summary of the invention
The object of the present invention is to provide tetramycin complex and application, to solve the above problems.
Embodiments providing a kind of tetramycin complex, comprising: tetramycin and active substance; Described active substance is ester class, described ester class comprise following one or more: dithiocarbamate, carbamates, pyrethroid.
The embodiment of the present invention additionally provides a kind of application of tetramycin complex, for controlling plant diseases, insect pest and weeds, and/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
The tetramycin complex that the embodiment of the present invention provides and application are compared with prior art, tetramycin possesses the effect of sterilization, control germ, disease, tetramycin and active substance composite after, by by having the active substance of the different mechanism of action and its combination, reduce refractory fungal bacterial strain optionally dangerous, service life of extending active component, add the fungicidal spectrum of single component, expand prevention and treatment range; Improve protection effect; Can delay or overcome the pesticide resistance of germ; Both saved the application times that cost again reduces agricultural chemicals, active response country advocates environmental protection agricultural chemicals.
Embodiment
Be described in further detail the present invention below by specific embodiment, content mentioned in embodiment is not limitation of the invention, and material prescription is selected to suit measures to local conditions and on result without substantial impact.In these embodiments, unless otherwise indicated, all percentage is all weight percentage.
Tetramycin complex comprises: tetramycin and active substance; Described active substance is ester class, described ester class comprise following one or more: dithiocarbamate, carbamates, pyrethroid.
The embodiment of the present invention additionally provides a kind of application of tetramycin complex, for controlling plant diseases, insect pest and weeds, and/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
Biopesticide tetramycin is a kind of high-effective broad-spectrum fungicide; to the plurality of plant diseases on the crops such as cereal, fruit tree, vegetables, all there is protection and therapeutic activity, especially to fungal diseases such as gray mold crops such as () tomato, cucumber, capsicums, powdery mildew (wheat class, melon, fruit tree etc.), scabs (apple, pears etc.), there is good control efficiency.But this series bactericidal agent action site is single, and germ easily produces adaptive variation to medicament, the preventive effect of medicament is reduced.Tetramycin and active substance composite after can play synergistic function, by by having the active substance of the different mechanism of action and its combination, reduce refractory fungal bacterial strain optionally dangerous, service life of extending active component, add the fungicidal spectrum of single component, expand prevention and treatment range; Improve protection effect; Can delay or overcome the pesticide resistance of germ; Both saved the application times that cost again reduces agricultural chemicals, active response country advocates environmental protection agricultural chemicals.
Next, the present invention will describe this tetramycin complex in detail by some specific embodiments, and the embody rule situation of tetramycin complex:
Tetramycin complex, comprising: tetramycin and active substance; Active substance comprise following one or more: dithiocarbamate, carbamates, pyrethroid.
In certain embodiments, when active substance comprises dithiocarbamate, dithiocarbamate comprise following one or more: ferbam, mancozeb, maneb, Carbatene, Propineb, tmtd, zineb, ziram;
In certain embodiments, when active substance comprises carbamates, carbamates comprise following one or more: sevin, carbofuran, Aphox, Pyramat, Mobucin, Bassa, Aldicarb, Methomyl, ordram, benthiocarb;
In certain embodiments, when active substance comprises pyrethroid, pyrethroid comprise following one or more: decis, cypermethrin, cyfloxylate, sumicidin, trichlorine cypermethrin, beta-cypermethrin;
In certain embodiments, when active substance also comprise following one or more other there is the compound of different structure: diazosulfide, irritated pathogenicity proteins, anilazine, blasticidin-S, chinomethionat, chloroneb, tpn, cymoxanil, dichlone, diclomezin, botran, the mould prestige of second, dimethomorph, Delan, Grandox fumigant, famoxadone, Fenamidone, fentin, ferimzone, fluazinam, flusulfamide, microbial inoculum is phonetic, aliette, dislike mould spirit, kasugarnycin, methasulfocarb, Pencycuron, rice blast peptide, polyoxin, probenazole, Propamocarb, pyroquilon, quinoxyfen, pcnb, Cosan, triazoxide, tricyclazole, triforine, valida, Propineb,
Wherein the mass concentration of tetramycin in tetramycin complex can adjust arbitrarily in 0.15-99%, according to the type of compound and required effect, reaches better balanced toxicity and residual by adjustment mass concentration (or the concentration that weighs).
The rate of application of inventive mixture is 0.6-400 gram/ha.
Such as: in certain embodiments, the mass concentration of tetramycin is 0.15%, and concentration is lower.
In certain embodiments, the mass concentration of tetramycin is 15%, or 35%, or 50%, or 70%.
In certain embodiments, the mass concentration of tetramycin is 99%, and concentration is higher, in tetramycin complex, play Main Function.
The finished product of this tetramycin complex can make in various forms one or more, such as: aqua, dispersible agent, missible oil, microemulsion, emulsion, suspension, gel formulation, ultra low volume solvent (ULV).Be made into various forms and facilitate the storage of tetramycin complex, transport and follow-up use.
Such as:
A) aqua
The composite mixture of 10 weight portion is dissolved in 90 weight parts waters or water-soluble solvent.Or add wetting agent or other auxiliary agent, composite mixture dissolves through water dilution, obtains the aqua that tetramycin complex mass percentage content is 10% in this way.
B) dispersible agent
Be dissolved in 70 weight portion cyclohexanone by the composite mixture of 20 weight portion, and add 10 parts per weight dispersing agent as PVP(polyvinyl pyrrolidone), dilute with water obtains dispersion, obtains the dispersible agent that tetramycin complex mass percentage content is 20% in this way.
C) missible oil
The composite mixture of 15 weight portion to be dissolved in 75 parts by weight of xylene and to add calcium dodecyl benzene sulfonate and castor oil ethoxylate (being 5 weight portions in each case), dilute with water obtains emulsion, obtains the missible oil that tetramycin complex mass percentage content is 15% in this way.
D) microemulsion
The composite mixture of 10 weight portion to be dissolved in 20 weight portion cyclohexanones and to add calcium dodecyl benzene sulfonate (being 20 weight portions in each case), dilute with water obtains microemulsion, obtains the microemulsion that tetramycin complex mass percentage content is 5% in this way.
E) emulsion
The composite mixture of 25 weight portion to be dissolved in 35 parts by weight of xylene and to add calcium dodecyl benzene sulfonate and castor oil ethoxylate (being 5 weight portions in each case).This mixture to be introduced in 30 weight parts waters by emulsifier (Ultraturrax) and make equal phase emulsion.Dilute with water obtains emulsion, obtains the emulsion that tetramycin complex mass percentage content is 25% in this way.
F) suspension
In the ball mill stirred, the composite mixture of 20 weight portion is pulverized and adds 10 parts per weight dispersing agent and wetting agent and 70 weight parts waters or organic solvent, obtain reactive compound suspension in small, broken bits, dilute with water obtains stable reactive compound suspension, obtains the suspension that tetramycin complex mass percentage content is 20% in this way.
G) gel formulation
In ball mill, the composite mixture of 20 weight portion, 10 parts per weight dispersing agent, 1 weight portion gelling agent and 70 weight parts waters or organic solvent grinding are obtained delicate suspensions, dilute with water is obtained the stabilized with mixture suspension that tetramycin complex mass percentage content is 20% in this way.
H) ultra low volume solvent
The composite mixture of 10 weight portion is dissolved in 90 weight parts organic solvent as in dimethylbenzene, obtains the product used without dilution that the composite mixture quality degree of tetramycin is 10%.
This tetramycin complex can be used for the mode controlling disease similar to other preparations, insect pest and weeds etc.Simultaneously compared with unitary agent, can controlling disease, insect pest and weeds better, play the effect of Synergistic.Both tetramycin complex can be used and also tetramycin and active substance can be used successively.
In the application of this tetramycin complex, may be used for for controlling plant diseases, insect pest and weeds, also for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
This tetramycin complex can be used as disease on the plant in control different season and plant part (fruit, flower, leaf, stem, stem tuber, root), insect pest, weeds, and also protective plant late growing stage part provides growth institute energy requirement from germ infringement and for plant.
The application target crop of the tetramycin complex of the present invention's protection generally includes but is not limited to following plants kind: cereal (wheat, barley, rye, oat, paddy rice, corn, Chinese sorghum and related species); Beet (sugar beet and fodder beet); The operatic circle, drupe and berry (kiwi fruit, strawberry, raspberry, gooseberry, fruit mulberry, fig, pomegranate, carambola, guava etc.); Leguminous plant (Kidney bean, little inclined beans, pea, soybean, red bean); Oil plants (rape, shepherd's purse, opium poppy, olive, sunflower, coconut, castor oil plant, cocoa bean, peanut); Mellon plant (pumpkin, cucumber, muskmelon, balsam pear, wax gourd, watermelon); Fibre plant (cotton, flax, hemp, jute); Citrus fruit (orange, lemon, glucose shaddock, bar tangerine); Vegetables (spinach, asparagus lettuce, asparagus, wild cabbage, carrot, onion, tomato, potato, red pepper); Lauraceae (avocado, Chinese cassia tree, camphor) or such as tobacco, nut, coffee, eggplant, sugarcane, tea, pepper, comprise grape, banana, the vining plant of grass roots and natural rubber plant and ornamental plants (flowers, shrub, broad-leaved tree and evergreen plant) etc.It should be noted that, this inventory does not represent any restriction.
Tetramycin complex of the present invention also can be used for control industrial materials fungal infection (especially by mould microbial), bacterium or insect pest, and safeguard industries material infects from fungal attack with at this fungal infection rear reduction occurring or eradicates industrial materials.Industrial materials include but not limited to organic and inorganic material timber, paper, leather, natural or synthetic fibers and composite, such as particle board, plywood, wallboard etc.The present invention can be applied to this material with the consumption effectively suppressed.
Composite mixture of the present invention is used for the Prevention and Curation that disease, worm, crop smothering occur in plant, seed, soil, region, material and spatial dimension, simultaneously for plant provides growth institute energy requirement.
In use, its application form is this tetramycin complex: any one in preparaton, spray solution, emulsion, paste, oil.Type of service depends on and is specifically intended to object; Under certain conditions, should guarantee that tetramycin complex is meticulous and distribute equably.
In certain embodiments, when the application form of tetramycin complex is preparaton, tetramycin complex and solvent and/or carrier and/or emulsifier and/or the blended rear use of surfactant.
In certain embodiments, when tetramycin complex and the blended rear use of solvent, solvent comprise following one or more: water, arsol (as toluene, dimethylbenzene), paraffin (as mineral oil fractions), alcohols (as methyl alcohol, butanols, amylalcohol, benzylalcohol), ketone (as cyclohexanone, gamma-butyrolacton), pyrrolidones (NMP, NOP), acetic acid esters (ethylene acetate), dihydroxylic alcohols, fatty acid dimethylamides, fatty acid and fatty acid ester.
In certain embodiments, when tetramycin complex and the blended rear use of emulsifier, emulsifier comprise following one or more: nonionic and anion emulsifier (as polyoxyethylene aliphatic alcohol ether, alkylsulfonate and arylsulphonate);
In certain embodiments, when tetramycin complex and the blended rear use of surfactant, surfactant comprise following one or more: lignosulphonic acid, naphthalene sulfonic acids, phenolsulfonic acid, the alkali metal salt of dibutyl naphthalenesulfonic acid, alkali salt, ammonium salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, aliphatic alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, the condensation product of naphthalene or naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, alkyl phenyl polyglycol ether, tributyl phenyl polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, alcohol and fatty alcohol/ethylene oxide condensation product, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylation polyoxypropylene, laruyl alcohol polyglycol ether acetal, sorbitol ester, lignin sulfite waste liquor, methylcellulose.
When tetramycin complex acts on plant seed, the mode of preparaton can be made into, except above-mentioned each blending constituent, can also mix with adhesive and/or gelling agent and/or colouring agent.
When the application form of tetramycin complex is spray solution, emulsion, paste, oil, the material of dispersion is that mid-boiling point arrives high boiling mineral oil fractions, as kerosene or diesel oil, in addition the oil of coal tar and plant or animal origin is also had, aliphatic series, ring-type and aromatic hydrocarbon, such as toluene, dimethylbenzene, paraffin, tetrahydronaphthalene, alkylated naphthalene or derivatives thereof, methyl alcohol, ethanol, propyl alcohol, butanols, cyclohexanol, cyclohexanone, isophorone, intensive polar solvent, such as dimethyl sulfoxide (DMSO), 1-METHYLPYRROLIDONE and water.
Tetramycin complex adopts moisture type of service to make emulsion concentrates, paste by adding water in the application.For preparation emulsion, paste or oil dispersion, can by wetting agent, thickener, dispersant or emulsifier by this material directly or to be dissolved in after in oil or solvent homogenizing in water.But, the concentrate that is made up of composite mixture, wetting agent, thickener, dispersant or emulsifier and suitable solvent or oil can also be prepared and this concentrate is suitable for dilute with water.
In certain embodiments, tetramycin complex can be used for controlling disease comprise following one or more: fungal disease, bacterial disease, virus disease, nematodosis.
When tetramycin complex is for preventing and treating fungal disease, fungal disease comprise following one or more: Deuteromycotina fungi, Basidiomycotina fungi, Ascomycotina fungi, Zygomycotina fungi, Mastigomycotina fungi;
When tetramycin complex is for preventing and treating bacterial disease, bacterial disease comprise following one or more: pseudomonas, bacillus;
When tetramycin complex is used for preventing and treating virus disease, virus disease comprise following one or more: double-stranded DNA virus, single-stranded DNA viruses, diplornavirus, single strand RNA virus;
When tetramycin complex be used for pest control time, insect pest comprise following one or more: insect, mite class, snail, muroid;
When tetramycin complex be used for controlling weeds time, weeds comprise following one or more: grass, cyperus, broad-leaf weeds;
Provide the specific embodiment of several tetramycin complex below, should understand, these embodiments are only for further illustrating of the present invention, and be not used in and limit the scope of the invention, without departing from the idea case in the present invention described above, the various replacement made according to ordinary skill knowledge and customary means or change, all should comprise within the scope of the invention.
Example one tetramycin and dimethomorph composite
Wherein, active material component selects dimethomorph.Tetramycin, the former medicine of dimethomorph are made 20% suspending agent respectively and carried out indoor pot experiment.Test method is carried out with reference to Wu Wenjun " chemical protection of plant experimental technique introduction " (1993).According to requirement of experiment, make single dose respectively, and the type of respective quality ratio.The concrete data of result of the test are in table 1.
Table 1 tetramycin and dimethomorph indoor pot experiment data.
Calculate the toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture according to the abundant method of Sun Yun, when CTC≤80 are antagonism, 80 ﹤ CTC ﹤ 120 are summation action, and CTC >=120 are synergistic effect.
Found by the test of indoor bacterium colony Developing restraint, tetramycin and dimethomorph all have synergistic effect to cucumber downy mildew afterwards according to the ratio of 3:1 ~ 1:5 is composite, and wherein obvious with 1:3 synergy, co-toxicity coefficient is 227.68, and the two compound synergic is remarkable.
20% tetramycin dimethomorph suspending agent:
0.3% tetramycin 4%, dimethomorph 16%, methyl naphthalene sulfonate formaldehyde condensate (dispersant) 5%, 0203B (surfactant) 6%, white carbon (filler) 5%, aluminium-magnesium silicate (suspending agent) 0.4%, ethylene glycol (antifreezing agent) 3%, tributyl phosphate (defoamer) 1.5%, deionized water complements to 100%.Above-mentioned formula is pulverized in proportion in advance, then adds in sand mill and grind, the obtained 20% tetramycin dimethomorph suspending agent of allotment after high speed shear mixing.
This formula control cucumber downy mildew is by 2000 times of dilution sprayings, and after medicine, 7 days control efficiency are 91.3%.6% tetramycin aqua is by 1500 times of dilution sprayings, and after medicine, 7 days control efficiency are that 81.2%, 40% dimethomorph water dispersion granula dilutes spraying by 1500 times, and after medicine, 7 days control efficiency are 69.7%.Test shows that 20% tetramycin dimethomorph suspending agent has good control efficiency to cucumber downy mildew, is better than corresponding single dose.
Example two tetramycin and azoxystrobin compound
Wherein, active material component selects Fluoxastrobin
Embodiment 2-1 preparation method: 18% tetramycin Fluoxastrobin missible oil:
Take 6% tetramycin, 12% Fluoxastrobin, 4% agriculture breast 500# (calcium dodecyl benzene sulfonate), 3%Span-60# (sorbitan monostearate), 3% agriculture breast 2201#, 2% triphenyl phosphite, dimethylbenzene mends 100%.Fully dissolve after above-mentioned raw materials mixing, the obtained 18% tetramycin Fluoxastrobin missible oil of mixing.
Embodiment 2-2 preparation method: 24% tetramycin Fluoxastrobin suspending agent:
Take 4% tetramycin, 20% Fluoxastrobin, 3% agriculture breast 600,3% agriculture breast 2201,3% lignosulfonates, 0.2% xanthans, 6% ethylene glycol, 0.5% silicone defoaming agent, add deionized water and mend to 100%.After above-mentioned raw materials is mixed, through the obtained 24% tetramycin Fluoxastrobin suspending agent of high speed shear emulsification.
Embodiment 2-3 preparation method: 12% tetramycin azoxystrobin microemulsion:
Take 3% tetramycin, 9% Fluoxastrobin, 6% emulsifier if agriculture breast 500# (calcium dodecyl benzene sulfonate), 2% agriculture breast 2201#, 3% agriculture breast 600#, 1% methyl alcohol, 6% dimethylbenzene, 1.2% epoxychloropropane, 0.4% aceticanhydride, deionized water benefit are to 100%, above-mentioned raw materials is fully dissolved the obtained 12% tetramycin azoxystrobin microemulsion of mixing.
Assays Example 2-1: to the indoor colony growth inhibition test of rice sheath blight disease.
Test method is carried out with reference to Wu Wenjun " chemical protection of plant experimental technique introduction " (1993).The tetramycin mother liquor of 15%, the former medicinal acetone of 95.4% Fluoxastrobin, Tween-80 are made 1.0 μ g/mL mother liquors, utilize that mother liquor becomes Fluoxastrobin, tetramycin mass ratio is respectively 3:1 ~ 5:1, gradient be respectively 0.01 μ g/mL, 0.02 μ g/mL, 0.04 μ g/mL, 0.08 μ g/mL PDA dull and stereotyped.The concrete data of result of the test are in table 2.
Table 2 Fluoxastrobin, tetramycin indoor antibacterial test data.
Calculate the toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture according to the abundant method of Sun Yun, when CTC≤80 are antagonism, 80 ﹤ CTC ﹤ 120 are summation action, and CTC >=120 are synergistic effect.
Found by indoor bacterium colony Developing restraint test, Fluoxastrobin, tetramycin long all have synergy inhibitory action according to composite rear to be born to Rhizoctonia solani Kuhn of the mass ratio of 3:1 ~ 5:1, wherein obvious with tetramycin, Fluoxastrobin 1:2 synergy, co-toxicity coefficient is 234.57, the two compound synergic is remarkable, more effectively can suppress the expansion of bacterium colony.
Assays Example 2-2: the field control effectiveness test of the water prevention rice sheath blight.
Reagent agent and dispenser dosage: (1) 24% tetramycin Fluoxastrobin suspending agent (preparation method embodiment 2-2), mu 40 grams, preparation; (2) 12% tetramycin azoxystrobin microemulsion (preparation method embodiment 2-3), mu 80 grams, preparation.
Contrast medicament and dispenser dosage: (3) 1.5% tetramycin aquas, mu 40 grams, preparation; (4) 10% Fluoxastrobin wetting powders, mu 20 grams, preparation,
Spraying time and application method: the dispenser of rice sheath blight disease their early stage, is watered even spraying.
The field control effectiveness test result of the table 3 water prevention rice sheath blight.
| Reagent agent | Preparation usage amount (g/ mu) | 7 days preventive effects (%) after medicine | 14 days preventive effects (%) after medicine |
| 1 | 40 | 74.3 | 81.3 |
| 2 | 80 | 73.8 | 80.7 |
| 3 | 40 | 66.5 | 72.5 |
| 4 | 20 | 61.5 | 71.9 |
Result of the test: in table 3.As can be seen from the test results: after dispenser 7 days, tetramycin of the present invention and Fluoxastrobin mixture composition to rice sheath blight disease preventive effect all more than 70%, significantly better than the single dose of contrast; After dispenser 14 days, tetramycin of the present invention and Fluoxastrobin mixture composition to rice sheath blight disease preventive effect all more than 80%, also significantly better than the single dose of contrast; In process of the test, each medicament is showed no poisoning generation, to crop safety.Illustrate that tetramycin of the present invention and Fluoxastrobin mixture composition are better than single dose, improve actual control efficiency, reduce dosage, reduce costs, reduce medicament to the adverse effect of environment, delay the generation of disease drug resistance, agricultural synthesis is administered and has great significance.
Example three tetramycin and carbendazim composite
Wherein, active material component selects carbendazim.The technical scheme adopted: a kind of tetramycin carbendazim suspending agent, comprises the component of following mass percent: tetramycin 21-27%, jinggangmeisu 2-6%, ethylene glycol 2-8%, n-octyl alcohol 0.45-0.55%, phenethyl phenol polyethenoxy ether 5-11%, xanthans 0.05-0.15%, water 53.4-63.4%.
The present invention adopts carbendazim, tetramycin, ethylene glycol, n-octyl alcohol, phenethyl phenol polyethenoxy ether, xanthans, water to be raw material, is mixed into tetramycin complex-tetramycin carbendazim suspending agent in proportion at normal temperatures.
Beneficial effect: the present invention compared with prior art, its remarkable advantage is: the active ingredient of product of the present invention is carbendazim and tetramycin, carbendazim, tetramycin and filler are re-dubbed by a certain percentage tetramycin carbendazim Recompounded pesticide, delay the pesticide resistance of crop, improve the control efficiency of agricultural chemicals, played the wherein strong point of various medicament, reduced cost, saving of labor's remarkable benefit, improves economic benefit.
Embodiment 3-1
The carbendazim of 21%, the tetramycin of 2%, ethylene glycol, the n-octyl alcohol of 0.45%, phenethyl phenol polyethenoxy ether, the xanthans of 0.15%, the water of 63.4% of 11% of 2% to be dropped in reactor mixing and stirring at normal temperatures and can obtain 23% described tetramycin carbendazim suspending agent.
Embodiment 3-2
The carbendazim of 24%, the tetramycin of 4%, ethylene glycol, the n-octyl alcohol of 0.5%, phenethyl phenol polyethenoxy ether, the xanthans of 0.1%, the water of 58.4% of 8% of 5% to be dropped in reactor mixing and stirring at normal temperatures and can obtain 28% described tetramycin carbendazim suspending agent.
Embodiment 3-3
The carbendazim of 27%, the tetramycin of 6%, ethylene glycol, the n-octyl alcohol of 0.55%, phenethyl phenol polyethenoxy ether, the xanthans of 0.05%, the water of 53.4% of 5% of 8% to be dropped in reactor mixing and stirring at normal temperatures and can obtain 33% described tetramycin carbendazim suspending agent.
Tetramycin, carbendazim and the compound control rice blast test of pesticide effectiveness thereof
This experimental establishment is at Jinan City, Shandong Province Pingying County, and test medicine is researched and developed by Liaoning Wkioc Bioengineering Co., Ltd., provided, contrast medicament 90% carbendazol wettable powder (commercial), 0.3% tetramycin aqua (company has by oneself).
Investigation rice blast state of an illness radix, the total number of sheets, the sick number of sheets and sick leaf-size class number before medicine, in their early stage dispenser, once, dispenser 2 times altogether, investigates disease index and calculates preventive effect for after second time dispenser 7 days, 14 days respectively in dispenser in every 7 days.Experimental result is as follows:
Table 4 tetramycin, carbendazim and the sick test of pesticide effectiveness of compound control rice blast thereof
As can be seen from Table 4, tetramycin carbendazim compound effectively can be prevented and treated rice blast and occur, and control efficiency is all better than contrasting medicament, has no adverse effects in this test scope of medication to target crop.
Example four tetramycin and mancozeb composite
Wherein, active material component selects mancozeb.
The described bactericidal composition containing tetramycin and mancozeb, the synergistic activity proportioning of tetramycin and mancozeb is 1: 1 ~ 1: 60, and preferred proportioning is 1: 1 ~ 1: 30, and preferred proportioning is 1: 5 ~ 1: 20.
The described bactericidal composition containing tetramycin and mancozeb, wherein effective active component content accounts for 1 ~ 85% of gross weight, is preferably 10 ~ 60%.
Composition comprises following constituent content when making suspending agent: tetramycin 1 ~ 15%, mancozeb 10 ~ 50%, dispersant 2 ~ 10%, wetting agent 2 ~ 10%, defoamer 0.1 ~ 1%, thickener 0.1 ~ 2%, antifreeze 0.1 ~ 8%, emulsifier 0.5% ~ 5%, deionized water add to 100%.
Dispersant, wetting agent, defoamer, thickener, antifreeze, emulsifier in above-mentioned formulation material are mixed through high speed shear, add tetramycin, mancozeb, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μm, obtained tetramycin mancozeb suspending agent product.
This tetramycin complex is mainly used in preventing and treating the fusarium wilt of vegetables and fruit tree, bacterial angular leaf spot, downy mildew, leaf mold and rice blast.
This tetramycin complex effective active composition tetramycin and mancozeb belong to the bactericide of two kinds of different mechanisms of action, and both can not produce conflict at mutual mixture;
This tetramycin complex has good synergistic effect within the specific limits, and preventive effect is higher than single dose, and dosage is little;
This tetramycin complex not with an organic solvent, not easily produces poisoning, is convenient to transport and storage;
This tetramycin complex has special efficacy to the fusarium wilt of vegetables and fruit tree, downy mildew, leaf mold and rice blast.
The method that the embodiment of the present invention adopts Toxicity Determination and field trial to combine.
Implementation of an application example 4-1: tetramycin and mancozeb compound are to cucumber fusarium axysporum indoor virulence test
Determine that after the effective inhibition concentration scope of each medicament, each medicament establishes 5 dosage process respectively by active constituent content through prerun, if clear water contrast.Carry out with reference to " farm-chemical indoor determination test rule bactericide ", adopt mycelial growth rate method to measure medicament to the virulence of cucumber fusarium axysporum.Measure colony diameter by right-angled intersection method after 72h, calculate each process net growth, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter-5
Mycelial growth inhibition rate (%)=control group net growth-processed group life only/control group net growth × 100
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) converts logarithm value (x) to, try to achieve virulence regression equation (y=a+bx) with method of least squares, and calculate the EC50 value of often kind of medicament thus.Calculate two medicament different ratio synergies ratio (SR) according to Wadley method, SR < 0.5 is antagonism, and 0.5≤SR≤1.5 are summation action simultaneously, and SR > 1.5 is synergistic effect.Computing formula is as follows:
SR=EC50 (theoretical value)/EC50 (observed value) × 100
EC50 (theoretical value)=a+b/ (EC50 of the EC50+b/B of a/A)
Wherein: a, b are respectively tetramycin and the ratio of mancozeb shared by combination.A is tetramycin, and B is mancozeb
Result of the test is as shown in table 5:
Table 5 tetramycin, mancozeb and compound thereof are to the toxicity test result of cucumber fusarium axysporum
As shown in Table 5, tetramycin, the EC50 of mancozeb to cucumber fusarium axysporum is respectively 2.35mg/L and 113.94mg/L.The virulence of tetramycin is higher than the virulence of mancozeb.The synergy ratio of tetramycin and mancozeb proportioning 1: 1,1: 15,1: 30,1: 45,1: 60 is respectively 1.56,1.89,1.91,1.73,1.51, illustrates that tetramycin and mancozeb mixture in 1: 1 ~ 1: 60 scopes all shows obvious synergistic effect.
Implementation of an application example 4-2: implement formula
Embodiment 4-2-1 48% tetramycin mancozeb suspending agent
Tetramycin 8%, mancozeb 40%, alkyl benzene sulfonate calcium salt 5%, Sodium Polyacrylate 2%, DBSA 4%, silicone oil 0.1%, xanthan gum 0.3%, phenyl phenol polyoxyethyl ether 2%, deionized water adds to 100%, the obtained 48% tetramycin mancozeb suspending agent of mixing.
Embodiment 4-2-2 40% tetramycin mancozeb suspending agent
Tetramycin 6%, mancozeb 34%, polycarboxylate 4%, soapberry powder 5%, silicone compound 0.1%, hydroxyethylcellulose 0.1%, ethylene glycol 2%, alkyl benzene calcium sulfonate 2%, deionized water adds to 100%, the obtained 40% tetramycin mancozeb suspending agent of mixing.
Embodiment 4-2-3 30% tetramycin mancozeb suspending agent
Tetramycin 15%, mancozeb 15%, polycarboxylate 2%, neopelex 2%, silicone compound 0.1%, polyethylene glycol 0.5%, glycerine 4%, phenethyl phenol polyoxyethanyl polypropylene-base ether 1%, deionized water adds to 100%, the obtained 30% tetramycin mancozeb suspending agent of mixing.
Implementation of an application example 4-3: tetramycin and mancozeb and compositely prevent and treat the cladosporium leaf and fruit mould of tomato test of pesticide effectiveness
This experimental establishment is in suburb, Lingyuan City, Liaoning Province, and test medicine is researched and developed by Liaoning Wkioc Bioengineering Co., Ltd., provided, contrast medicament 80% mancozeb wettable powder (commercial), 1.5% tetramycin aqua (company provides).
The investigation cladosporium leaf and fruit mould of tomato state of an illness before medicine, in the first time dispenser of state of an illness initial stage, dispenser in every 7 days once, is total to dispenser 3 times.Within after third time dispenser 7 days, 14 days, 30 days, investigate cladosporium leaf and fruit mould of tomato feelings index respectively and calculate preventive effect.Experimental result is as follows:
Table 6 tetramycin, mancozeb and compound thereof prevent and treat the cladosporium leaf and fruit mould of tomato test of pesticide effectiveness
As can be seen from Table 6, tetramycin and mancozeb composite after effectively can prevent and treat cladosporium leaf and fruit mould of tomato, control efficiency be all better than contrast medicament, the lasting period is long.In test scope of medication, target crop is had no adverse effects.
Implementation of an application example 4-4: tetramycin and mancozeb and compound thereof prevent and treat the cucumber downy mildew test of pesticide effectiveness
This experimental establishment is at Jinan City, Shandong Province suburban area, and test medicine is researched and developed by Liaoning Wkioc Bioengineering Co., Ltd., provided, contrast medicament 80% mancozeb wettable powder (commercial), 1.5% tetramycin aqua (company provides).
The investigation cucumber downy mildew state of an illness before medicine, in the first time dispenser of state of an illness initial stage, dispenser in every 7 days once, is total to dispenser 2 times.Within after second time dispenser 7 days, 14 days, 30 days, investigate cucumber downy mildew disease index respectively and calculate preventive effect.Experimental result is as follows:
Table 7 tetramycin, mancozeb and the compound control cucumber downy mildew test of pesticide effectiveness thereof
As can be seen from Table 7, tetramycin and mancozeb composite after can effectively preventing cucumber downy mildew, control efficiency is all better than contrasting medicament, and dosage is little, and the lasting period is long.Test scope of medication in target crop without harm.
Implementation of an application example 4-5: tetramycin, mancozeb and the compound control cucumber bacterial angular leaf spot test of pesticide effectiveness thereof
This experimental establishment is fated in Lingyuan City, Liaoning Province four town, test medicine is researched and developed by Liaoning Wkioc Bioengineering Co., Ltd., is provided, contrast medicament 80% mancozeb wettable powder (commercial), 1.5% tetramycin aqua (company has by oneself).
Investigation cucumber bacterial angular leaf spot state of an illness radix, the total number of sheets, the sick number of sheets and sick leaf-size class number before medicine, in their early stage dispenser, once, dispenser 2 times altogether, investigates disease index and calculates preventive effect for after second time dispenser 7 days, 14 days respectively in dispenser in every 7 days.
Experimental result is as follows:
Table 8 tetramycin, mancozeb and the compound control cucumber bacterial angular leaf spot test of pesticide effectiveness thereof
As can be seen from Table 8, tetramycin and mancozeb composite after effectively can prevent and treat cucumber bacterial angular leaf spot and occur, control efficiency is all better than contrasting medicament, has no adverse effects in this test scope of medication to target crop.
Example the May 4th mycin and chlorothalonil compound
Wherein, active material component selects tpn, its chemical name: daconil M (2,4,5,6 one tetrachloro one 1,3 one benzene dicarbonitrile) is wide spectrum, protective fungicide.The mechanism of action can be had an effect with the glyceraldehyde 3-phosphate dehydrogenase in fungal cell, ties mutually entirely, thus destroy this enzymic activity, the metabolism of fungal cell is damaged and the power that loses one's life with the protein containing cysteine in this enzyme.Tpn does not have Uptake and translocation effect, but after being sprayed onto on plant corpus, can have good tackness on body surface, not easily be fallen by rain drop erosion, therefore the drug effect phase is longer.
For preventing and treating fruit tree, vegetables, the multiple fungal disease of cereal crop, be particularly useful for preventing and treating wheat powdery mildew.
With embodiment, content of the present invention is further described below, but the present invention is not limited thereto.
Embodiment 5-1
Tetramycin and tpn mixture are to the toxicity test of wheat powdery mildew
1) medicament preparation
Tetramycin solution: accurately take tetramycin mother liquor be diluted to 25,12.5,6.25,3.125,1.5625mg/L concentration is for subsequent use.
Tpn solution: accurately taking 0.01g weight concentration is the former medicine of 97% tpn, dissolve with 0.2ml dimethyl formamide, add the clear water 97ml containing 0.1% Tween 80 surfactant, stir, be mixed with tpn 100mg/L mother liquor, then with being diluted to 50 containing the clear water of 0.1% Tween 80 surfactant, 25,12.5,6.25,3.125mg/L concentration is for subsequent use.
Each mixture solution: get 5 respectively, 3,3,3,2ml 100mg/L tetramycin solution, add 5 respectively, 9,15,21, make 50mg/L tetramycin in 18ml 100mg/L tpn solution: tpn 1:1,1:3,1:5,1:7,1:9 solution, then with the clear water containing 0.1% Tween 80 surfactant be diluted to 25,12.5,6.25,3.125,1.5625mg/L concentration is for subsequent use.
Contrast solution: 0.2ml dimethyl formamide, adds the clear water of 96ml containing 0.1% Tween 80 surfactant.
2) experimental technique: with reference to romaine lettuce standard method NY/T 1156.4-2006, adopt pot-culture method: sprayed by the dissolution homogeneity prepared on wheat seedling for subsequent use, after liquid natural air drying, after chemicals treatment 24 hours, the wheat powdery mildew Fresh spores produced in morbidity wheat leaf blade upper 24 hour is evenly shaken off to be inoculated on the wheat seedling of process.Each process is no less than 3 basins, the strain of every basin 10.Experiment establishes the process not containing medicament as blank.Inoculation is placed on temperature 25 DEG C, cultivates in the greenhouse of relative moisture 70 ~ 90%.7 days " Invest, Then Investigate " sick numbers of sheets at different levels, SAS6.12 statistical software is adopted to analyze, experimentally data calculate disease index and control efficiency, obtain virulence regression equation, correlation coefficient (r) and 95% confidence interval (EC50), with reference to NY/T 1156.6-2006, the synergistic effect of pharmacy mix is evaluated according to the co-toxicity coefficient method (CTC) of Sun & Johnson (1960), namely CTC≤80 are antagonism, 80 < CTC < 120 summation actions, CTC >=120 synergistic effect.
3) evaluation method and evaluation criterion
Tetramycin and tpn and the combination of the two different proportion mixture the results are shown in Table 9 to the toxicity test of wheat powdery mildew.From table 9, in the mixture of 1:1,1:3,1:5,1:7,1:9 of tetramycin and tpn combines, all synergistic effect is showed to wheat powdery mildew, especially combine synergistic effect with the mixture of tetramycin and tpn 1:5 the most obvious.
Table 9 tetramycin and tpn mixture are to the toxicity test result of wheat powdery mildew
Formula: (percentage by weight) tpn 25%, tetramycin 5%, polycarboxylate 2%, aliphatic acid polyethenoxy ether 4%, dodecyl phenenyl sulfate 4%, organosilicon ketone 0.2%, carboxymethyl cellulose 1%, glycerine 1%, deionized water 57.8%.Mix, through ball milling, obtain 50% tetramycin tpn suspending agent.
Tetramycin and tpn and composite control wheat powdery mildew field efficacy thereof are tested
The investigation fresh kidney beans gray mold state of an illness before medicine, at wheat powdery mildew their early stage, once, dispenser twice altogether, observes disease index and calculates preventive effect, and adding up end product quality and output for after last medicine 14 days in dispenser in every 7 days.Experimental result is in table 10.
Table 10 tetramycin and tpn and complex preparation thereof prevent and treat the effect experiment of wheat powdery mildew
Field efficacy explanation, the complex preparation of tetramycin of the present invention and tpn, to wheat powdery mildew, there is good control efficiency, comparatively single dose has obvious synergistic effect, and the lasting period is long, wheatear grain is fuller, and wheat yield obviously increases production, amount of increase in production reaches more than 10%, is comparatively ideal bactericide.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a tetramycin complex, is characterized in that, comprising: tetramycin and active substance; Described active substance is ester class, described ester class comprise following one or more: dithiocarbamate, carbamates, pyrethroid.
2. tetramycin complex according to claim 1, is characterized in that, the mass concentration of described tetramycin in described tetramycin complex is 0.15-99%.
3. tetramycin complex according to claim 1, is characterized in that, described tetramycin complex is any one or more in aqua, dispersible agent, missible oil, microemulsion, emulsion, suspension, gel formulation, ultra low volume solvent.
4. the tetramycin complex according to any one of claim 1-3, it is characterized in that, when described active substance comprises dithiocarbamate, described dithiocarbamate comprise following one or more: ferbam, mancozeb, maneb, Carbatene, Propineb, tmtd, zineb, ziram;
When described active substance comprises carbamates, described carbamates comprise following one or more: sevin, carbofuran, Aphox, Pyramat, Mobucin, Bassa, Aldicarb, Methomyl, ordram, benthiocarb;
When described active substance comprises pyrethroid, described pyrethroid comprise following one or more: decis, cypermethrin, cyfloxylate, sumicidin, trichlorine cypermethrin, beta-cypermethrin.
5. the application of the tetramycin complex described in any one of claim 1-4, is characterized in that, for controlling plant diseases, insect pest, weeds; And/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
6. the application of tetramycin complex according to claim 5, is characterized in that, the application form of described tetramycin complex is: any one in preparaton, spray solution, emulsion, paste, oil.
7. the application of tetramycin complex according to claim 6, it is characterized in that, when the application form of described tetramycin complex is preparaton, described tetramycin complex and solvent and/or carrier and/or emulsifier and/or the blended rear use of surfactant.
8. the application of tetramycin complex according to claim 7, is characterized in that,
When described tetramycin complex and the blended rear use of solvent, described solvent comprise following one or more: water, arsol, paraffin, alcohols, ketone, pyrrolidones, acetic acid esters, dihydroxylic alcohols, fatty acid dimethylamides, fatty acid and fatty acid ester;
When described tetramycin complex and the blended rear use of carrier, described carrier comprise following one or more: the kaolin ground, the clay ground, the talcum ground, the chalk ground, the silica ground, the silicate ground;
When described tetramycin complex and the blended rear use of emulsifier, described emulsifier comprise following one or more: nonionic and anion emulsifier;
When described tetramycin complex and the blended rear use of surfactant, described surfactant comprise following one or more: lignosulphonic acid, naphthalene sulfonic acids, phenolsulfonic acid, the alkali metal salt of dibutyl naphthalenesulfonic acid, alkali salt, ammonium salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, aliphatic alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, the condensation product of naphthalene or naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, alkyl phenyl polyglycol ether, tributyl phenyl polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, alcohol and fatty alcohol/ethylene oxide condensation product, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylation polyoxypropylene, laruyl alcohol polyglycol ether acetal, sorbitol ester, lignin sulfite waste liquor, methylcellulose.
9. the application of tetramycin complex according to claim 5, is characterized in that, for described disease comprise following one or more: fungal disease, bacterial disease, virus disease, nematodosis.
10. the application of tetramycin complex according to claim 9, is characterized in that,
When described tetramycin complex is for preventing and treating fungal disease, described fungal disease comprise following one or more: Deuteromycotina fungi, Basidiomycotina fungi, Ascomycotina fungi, Zygomycotina fungi, Mastigomycotina fungi;
When described tetramycin complex is for preventing and treating bacterial disease, described bacterial disease comprise following one or more: pseudomonas, bacillus;
When described tetramycin complex is used for preventing and treating virus disease, described virus disease comprise following one or more: double-stranded DNA virus, single-stranded DNA viruses, diplornavirus, single strand RNA virus;
When described tetramycin complex be used for pest control time, described insect pest comprise following one or more: insect, mite class, snail, muroid;
When described tetramycin complex be used for controlling weeds time, described weeds comprise following one or more: grass, cyperus, broad-leaf weeds.
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| CN105665434A (en) * | 2016-01-20 | 2016-06-15 | 潍坊市新友吉土壤消毒服务专业合作社 | Soil disinfection and soil remediation combined technology |
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