CN103858874B - Application of natural compound as agricultural bactericide - Google Patents
Application of natural compound as agricultural bactericide Download PDFInfo
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- CN103858874B CN103858874B CN201410009288.9A CN201410009288A CN103858874B CN 103858874 B CN103858874 B CN 103858874B CN 201410009288 A CN201410009288 A CN 201410009288A CN 103858874 B CN103858874 B CN 103858874B
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Abstract
The invention discloses a natural compound Auraptenol used as a bactericide for preventing and treating agricultural fungal diseases, and the natural compound has a remarkable prevention and treatment effect especially on sclerotinia rot of colza, fusarium wilt of cucumber, a rice sheath blight disease, wheat scab, pepper anthracnose, a rice bakanae disease, wheat sharp eyespot, tomato late blight, gray mold and the like. As an agricultural bactericide, the Auraptenol has the characteristics of good effect, broad spectrum, low toxicity, no residues, no pollution and no harm, and is especially beneficial to environmental protection.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to the purposes of a kind of native compound Auraptenol as active fraction preparation agricultural bacteriocide.
Background technology
In agricultural production, the pathogen of damage to crops also has fungi, bacterium, virus etc., wherein the having the greatest impact of fungi, and crop production reduction is obvious, and the economic loss especially caused by fungus-caused disease because of plant disease can not be ignored.
Auraptenol, No. CAS: 1221-43-8, chemical name: (+)-8-(2-Hydroxy-3-methyl-3-butenyl)-7-methoxy-2H-1-benzopyran-2-one, molecular structure:
Auraptenol is colorless needle crystals, is dissolved in the organic solvents such as methyl alcohol, ethanol, acetone and chloroform, little to its activity research, does not have it so far as bactericide at the pertinent literature agriculturally applied or report.
Summary of the invention
The object of the invention is to explore Auraptenol and preparing the application of agricultural bacteriocide as active component.
The present invention is proved by large quantity research, Auraptenol also has excellent bactericidal activity as the agricultural bacteriocide that active fraction preparation obtains, can be applicable to the disease on the various crops of control, be particularly suitable for preventing and treating agricultural fungal diseases, as: sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold etc.
Auraptenol is that separation and purification obtains or synthesize obtaining from natural plants.Auraptenol is mixed with any one formulation that agricultural chemicals allows as active component and insecticides adjuvant, excipients.Auraptenol can also and other agriculture chemical compounding, be mixed and made into insecticides adjuvant, excipients any one formulation that agricultural chemicals allows.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Auxiliary agent used can adopt calcium dodecyl benzene sulfonate, pyrrolidones, the rare block compound of polyoxyethylene polyoxy third, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other agricultural chemicals used are organic phosphates, as: thimet (3911), demeton (1059, E1059), parathion (1605, E605), dichlorvos (DDV), Rogor, chlorophos etc.; Pyrethroid, as: cypermethrin, decamethrin, kill bacterium fat etc.; Microbial source insecticide, as bacillus thuringiensis,Bt (Bt), Avermectin, white muscardine fungi etc.
Beneficial effect of the present invention: Auraptenol is as bactericide compared with the prior art, and antimicrobial spectrum is wide, and the antibacterial time is short, and the lasting period is long, is agriculturally desirable bactericide.Compared with general chemical pesticide, Be very effective, Auraptenol derives from the extract of natural plants, has low toxicity, noresidue, pollution-free, non-harmful feature for economic crops such as various grain, melon and fruit, vegetables, more favorable environment protection.
Embodiment
Below by way of embodiment, effect of the present invention is described further:
Embodiment 1:Auraptenol is to the control of agricultural fungal diseases
Strains tested Fusarium graminearum (Fusarium.graminearm) is separated from wheat scab fringe and obtains; Fusarium oxysporum (F.oxysporum) is separated from cucumber fusarium axysporum diseased plant and obtains; Rhizoctonia solani Kuhn (Rhizoctonia solani) is separated from rice sheath blight disease leaf and obtains; Rhizoctonia cerealis (Rhizoctonia solani) is separated from wheat sharp eyespot leaf and obtains; Sclerotinite (Sclerotioia selerotiorum) is separated from sclerotinia rot of colza bacterial strain and obtains; Fusarium moniliforme (F.moniliform) is separated from bakanae disease of rice fringe; Capsicum anthrax-bacilus (Colletotrichum capsici) is separated from the sick leaf of capsicum; Botrytis cinerea (Botrytis cinerea) is separated from cucumber; Sporulation (Alternariasolani) is separated from the sick leaf of early blight of tomato; Phytophthora capsici (phytophthoracapsici) is separated from capsicum diseased plant.Reagent agent Auraptenol (being separated qualification to obtain from the samphire root of Dahurain angelica), be dissolved in acetone and make 1000 μ g/mL mother liquors, 4 DEG C save backup.PDA medium: 200g peeled potatoes is cut into block, after boiling 10min, gets juice, adds 20g agar, is heated to fusing, then adds 20g glucose, be settled to 1000mL with water.
Mycelial growth rate determination method is adopted to measure the suppression of Fungal hyphal growth, from PDA flat board, pre-incubated fungi edge is beaten and is got bacterium dish (diameter 5mm), by reagent agent mother liquor and medium Homogeneous phase mixing, make reagent agent ultimate density be respectively 0,1,2.5,5,10ppm, mixed liquor respectively impouring diameter is make flat board in the sterilizing culture dish of 9cm, a bacterium dish inoculated by every ware, and mycelia faces up, and often processes repetition 3 times.25 DEG C are cultured to contrast bacterium colony when being covered with culture dish more than 2/3rds, and right-angled intersection method is measured bacterium colony and increased diameter.Growth inhibition ratio is calculated by colony diameter mean value (mm).To the data DPS V3.01 professional version software analysis process of gained, draw calibration curve and EC
50value.Positive reference substance is Osthole (purchased from Nat'l Pharmaceutical & Biological Products Control Institute), calculates conk inhibiting rate with same concentration same method.
Can find out that from result Auraptenol can prevent and treat sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold etc. effectively, concentration EC during this several fungal disease is suppressed
50be 2.84 ~ 18.46ppm, specific experiment the results are shown in Table 1; And positive reference substance Osthole is to concentration EC in sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, these 5 kinds of fungal diseases suppression of wheat sharp eyespot
50be respectively 43.33,41.82,26.84,56.94,39.96ppm, fully demonstrated the remarkable result of agricultural chemicals of the present invention by contrast experiment.
Table 1Auraptenol is to agriculture fungi toxicity test result
Embodiment 2:
Take Auraptenol and the customary adjuvant such as lignin, diatomite conveniently technique, make the wetting powder that active ingredient concentration is 2%.
Embodiment 3:
Take Auraptenol and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, solvent wet goods customary adjuvant conveniently technique, make the missible oil that active ingredient concentration is 2%.
Embodiment 4:
Claim Auraptenol and the customary adjuvant such as wooden sodium sulfonate, kaolin conveniently technique, make the water dispersible granules that active ingredient concentration is 2%.
Embodiment 5:
Take Auraptenol and solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 1%.
Embodiment 6:
Take Auraptenol and the customary adjuvant such as calcium dodecyl benzene sulfonate, pyrrolidones conveniently technique, make the microemulsion that active ingredient concentration is 1%.
Embodiment 7:
Take Auraptenol and thimet according to the ratio of weight ratio 1: 5, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 12%.
Embodiment 8:
Take Auraptenol and decamethrin according to the ratio of weight ratio 6: 1, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 21%.
Embodiment 9:
Take Auraptenol and Avermectin according to the ratio of weight ratio 1: 3, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 4%.
Claims (3)
1. a native compound is as the purposes of bacteriostatic active ingredients for the preparation of agricultural bacteriocide, the agricultural disease of control is fungal disease, be specially sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold, wherein said native compound name is called Aurantenol, No. CAS: 1221-43-8, chemical name: 8-(2-Hydroxy-3-methyl-3-butenyl)-7-methoxy-2H-1-benzopyran-2-one, molecular structure is:
。
2. purposes according to claim 1, is characterized in that Auraptenol is mixed with any one formulation that agricultural chemicals allows as active component and insecticides adjuvant, excipients; Or Auraptenol is mixed with as active component and other agricultural chemicals and insecticides adjuvant, excipients any one formulation that agricultural chemicals allows.
3. purposes according to claim 2, is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule.
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CN201410009288.9A CN103858874B (en) | 2013-04-16 | 2013-04-16 | Application of natural compound as agricultural bactericide |
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CN103858874B true CN103858874B (en) | 2015-04-08 |
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CN105028047A (en) * | 2015-06-30 | 2015-11-11 | 云南天质网络科技有限公司 | Control method for rice gibberellic disease |
CN105432631B (en) * | 2015-11-24 | 2017-12-12 | 江苏省中国科学院植物研究所 | A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide |
CN106748787A (en) * | 2016-04-23 | 2017-05-31 | 何华琼 | A kind of agricultural bacteriocide and preparation method thereof |
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CN102093322A (en) * | 2010-12-31 | 2011-06-15 | 厦门大学 | Application of osthole in preparation of marine antifouling paint |
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Address after: 211225 Lishui City, Nanjing province Baima Town National Agricultural Science and Technology Park plant base Applicant after: Institute of Botany, Jiangsu Chinese Academy of Sciences Address before: 210014, No. 1, former village, Xuanwu District, Xuanwu District, Jiangsu, Nanjing Applicant before: Institute of Botany, Jiangsu Chinese Academy of Sciences |
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