CN105432631B - A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide - Google Patents
A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide Download PDFInfo
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- CN105432631B CN105432631B CN201510819082.7A CN201510819082A CN105432631B CN 105432631 B CN105432631 B CN 105432631B CN 201510819082 A CN201510819082 A CN 201510819082A CN 105432631 B CN105432631 B CN 105432631B
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- libanorin
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- agricultural chemicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of natural purposes of the angle-furocoumarins compound libanorin as agricultural bacteriocide.Compound libanorin is used to prevent and treat agricultural fungal evil as bactericide, the corps diseases such as sclerotinia sclerotiorum, rice sheath blight disease, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato can be effectively prevented and treated, it is especially obvious to fungal disease sclerotinia sclerotiorum, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato prevention effect.Native compound libanorin has that prevention effect is good as agricultural bacteriocide made of active component, broad spectrum activity and low toxicity, noresidue, pollution-free, non-harmful feature, is particularly conducive to environmental protection.
Description
Technical field
The invention belongs to agricultural technology field, and in particular to a kind of natural angle-furocoumarins compound libanorin
The purposes of agricultural bacteriocide is prepared as active component.
Background technology
The disease of crops due to easily being discovered in time unlike insect and weeds by people, so often resulting in preventing and treating
On ignorance and difficulty, thus its harm just it is even more serious.It is reported that whole world list is the plant disease as caused by disease fungus
Just up to 10,000 kinds, caused loss accounts for the 10%~30% of crop year total losses.If virus, bacterium and nematode etc. are drawn
Including the plant disease risen is also calculated, its loss is just more considerable.
In order to meet people's needs growing to grain and other living materials, it has to use chemicals to protect
Crop is not attacked by harmful organism, and wherein bactericide plays an important role.Natural products with agricultural bactericidal activity, typically
Easily it is absorbed by plants and conducts, it is small to phyto toxicity or without poisoning, therefore by attention.
Compound English name libanorin, molecular formula：C19H20O5, molecular weight 328.3591, CAS No. 27303-
26-0, from samphire Kingsoft Radix Angelicae Sinensis (Angelica valida Diels) molecular structure：
Compound libanorin, it is dissolved in the organic solvents such as methanol, ethanol, acetone, ethyl acetate, chloroform.Its activity is made
With study it is less, so far without as agricultural bacteriocide in the pertinent literature or report agriculturally applied.
The content of the invention
The purpose of the present invention is to explore compound libanorin to prepare the application of agricultural bacteriocide as active component.
The present invention proves that compound libanorin sterilizes as the agricultural that active component is prepared by numerous studies
Agent has excellent bactericidal activity, can be applicable to the fungal disease prevented and treated on various crops, such as：Sclerotinia sclerotiorum, rice
Banded sclerotial blight, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato etc..Especially to fungal disease rape sclerotium
Disease, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato prevention effect are obvious.
Compound libanorin is to isolate and purify to obtain from natural plants, can also be obtained by chemical synthesis process.Change
Any one formulation that compound libanorin is configured to allow on agricultural chemicals as active component and insecticides adjuvant, excipients.It is also
Any one formulation allowed on agricultural chemicals can be mixed and made into insecticides adjuvant, excipients with other agriculture chemical compoundings.Described agent
Type can be missible oil, suspending agent, aqueous emulsion, wettable powder, microemulsion or water dispersion granule etc..Auxiliary agent used can be adopted
With calcium dodecyl benzene sulfonate, pyrrolidones, polyoxyethylene polyoxy propylene block compound, NPE etc., assign
Shape agent can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc..Compounding other agricultural chemicals used can be organic
Bactericide class, such as triazole bactericidal agent Triadimenol；Botanical fungicide, such as allicin.
Beneficial effects of the present invention compared with the prior art：For compound libanorin as bactericide, antimicrobial spectrum is wide, holds
The effect phase is grown, and is agriculturally preferable bactericide.Learned to farm medicine phases ratio with vague generalization, it is right due to the extract from natural plants
There is low toxicity, noresidue, pollution-free, non-harmful feature, more favorable environment in industrial crops such as various grains, melon fruits and vegetables
Protection.
Embodiment
Effect of the present invention is described further below by way of embodiment：
Embodiment 1：Preventing and treatings of the compound libanorin to agricultural disease
Strains tested sclerotinite (Sclerotioia selerotiorum) is isolated from sclerotinia sclerotiorum bacterial strain；It is vertical
Withered silk kernel fungus (Thanatephorus cucumeris) separate from rice sheath blight disease disease leaf；Botrytis cinerea (Botrytis
), cinerea also known as the pathogen of Botrytis cinerea is isolated from grey mould fruit rot of strawberry；Fusarium graminearum (Fusarium.graminearm)
It is isolated from wheat scab fringe；Rhizoctonia cerealis（Rhizoctonia solani）Separated from wheat sharp eyespot leaf
Arrive；Phytophthora capsici (phytophthora capsici) separates from capsicum diseased plant；Capsicum thorn disk spore [Colletotrichum
Capsici (syd.) Butl.] it is isolated from pepper anthracnose fruit；Sporulation（Alternaria
solani.）It is isolated from early blight of tomato leaf.
Test compound libanorin（Samphire Kingsoft Radix Angelicae Sinensis is located away from, is compound through Spectral Identification
Libanorin, it is dissolved in methanol and 1000 μ g/mL mother liquors is made, 4 DEG C save backup.PDA culture medium:200g peeled potatoes
Block is cut into, after boiling 10min, juice is taken, adds 20g agar, be heated to melting, add 20g glucose, be settled to water
1000mL。
Determined using suppression of the mycelial growth rate determination method to mycelial growth, preculture supplies examination bacterium from PDA plate
Edge, which is beaten, takes bacterium dish (diameter 5mm), and reagent agent mother liquor is uniformly mixed with culture medium, makes the reagent agent ultimate density be
30ppm, mixed liquor are poured into a diameter of 9cm sterilizing culture dish and flat board are made, and a bacterium dish are inoculated with per ware, mycelia is face-up, often
Processing is repeated 3 times.When 25 DEG C of cultures to control bacterium colony is covered with culture dish more than 2/3rds, crossing method measurement bacterium colony increases
Diameter.Growth inhibition ratio is calculated by colony diameter average value (mm).
Using Osthole Osthol as positive reference substance, compound libanorin can be effectively as can be seen from the results
Prevent and treat sclerotinia sclerotiorum, rice sheath blight disease, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato etc., concentration
30ppm is respectively 70.00%, 26.88%, 68.79%, 54.27%, 42.87%, 38.43% to this several disease suppression rate, specific real
Test and the results are shown in Table 1；The remarkable result of agricultural chemicals of the present invention is fully demonstrated by experiment, especially to fungal disease rape sclerotium
Disease, rice sheath blight disease and pepper anthracnose prevention effect are obvious.
The compound libanorin of table 1（Concentration is 30ppm）To the bacteriostasis rate of agricultural disease
Embodiment 2：
Active ingredient is made according to common process in the customary adjuvant such as Weigh Compound libanorin and lignin, diatomite
Concentration is 2% wettable powder.
Embodiment 3：
Weigh Compound libanorin and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, solvent naphtha etc. are conventional
The missible oil that effective constituent concentration is 2% is made according to common process in auxiliary material.
Embodiment 4：
The customary adjuvant such as Weigh Compound libanorin and wooden sodium sulfonate, kaolin is made effectively according to common process
Constituent concentration is 2% water dispersible granules.
Embodiment 5：
Weigh Compound libanorin is with the customary adjuvant such as solvent naphtha, pyrrolidones, xanthans according to common process, system
Into the aqueous emulsion that active ingredient concentration is 1%.
Embodiment 6：
The customary adjuvant such as Weigh Compound libanorin and calcium dodecyl benzene sulfonate, pyrrolidones according to common process,
The microemulsion that effective constituent concentration is 1% is made.
Embodiment 7：
According to weight than 1：5 ratio Weigh Compound libanorin and Triadimenol, with solvent naphtha, pyrrolidones, xanthan
The aqueous emulsion that effective constituent concentration is 5% is made according to common process in the customary adjuvants such as glue.
Embodiment 8：
According to weight than 6：1 ratio Weigh Compound libanorin and allicin, with solvent naphtha, pyrrolidones, xanthan
The aqueous emulsion that effective constituent concentration is 5% is made according to common process in the customary adjuvants such as glue.
Claims (3)
1. compound libanorin as active component prepare preventing and treating sclerotinia sclerotiorum, rice sheath blight disease, grey mould fruit rot of strawberry,
Purposes in the agricultural bacteriocide of wheat scab, pepper anthracnose and early blight of tomato.
2. purposes according to claim 1, it is characterised in that compound libanorin helps as active component and agricultural chemicals
Agent, excipients are configured to any one formulation allowed on agricultural chemicals；Or compound libanorin is as active component and other
Agricultural chemicals and insecticides adjuvant, excipients are configured to any one formulation allowed on agricultural chemicals.
3. purposes according to claim 2, it is characterised in that described formulation is missible oil, suspending agent, aqueous emulsion, wettable
Pulvis, microemulsion or water dispersion granule；Other described agricultural chemicals are that triazole bactericidal agent Triadimenol or botanical fungicide are big
Allicin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510819082.7A CN105432631B (en) | 2015-11-24 | 2015-11-24 | A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide |
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| CN201510819082.7A CN105432631B (en) | 2015-11-24 | 2015-11-24 | A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide |
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| CN105432631A CN105432631A (en) | 2016-03-30 |
| CN105432631B true CN105432631B (en) | 2017-12-12 |
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| CN113632799A (en) * | 2020-04-27 | 2021-11-12 | 兰州大学 | Application of praeruptorin A in preventing and treating agricultural diseases |
| CN114656428A (en) * | 2020-12-23 | 2022-06-24 | 浙江清华长三角研究院 | New structural compound for resisting plant pathogenic fungi |
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| CN103141491B (en) * | 2013-03-26 | 2015-05-27 | 江苏省中国科学院植物研究所 | Applications of natural compound as agriculture bactericide |
| CN103858874B (en) * | 2013-04-16 | 2015-04-08 | 江苏省中国科学院植物研究所 | Application of natural compound as agricultural bactericide |
| CN105028435B (en) * | 2015-07-01 | 2017-03-01 | 江苏省中国科学院植物研究所 | A kind of native compound is as the purposes of agricultural bacteriocide |
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