CN113207878B - Application of magnolol as agricultural bactericide - Google Patents
Application of magnolol as agricultural bactericide Download PDFInfo
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- CN113207878B CN113207878B CN202110454042.2A CN202110454042A CN113207878B CN 113207878 B CN113207878 B CN 113207878B CN 202110454042 A CN202110454042 A CN 202110454042A CN 113207878 B CN113207878 B CN 113207878B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses application of a compound magnolol as an agricultural bactericide. The compound is used as a bactericide for preventing and treating agricultural fungal diseases, and can effectively prevent and treat crop diseases such as sclerotinia rot, banded sclerotial blight, gray mold, gibberellic disease, anthracnose, bakanae disease, gummy stem blight, fusarium wilt, verticillium wilt and the like. The agricultural bactericide prepared by magnolol as an active ingredient has the characteristics of good prevention and treatment effect, broad spectrum, low toxicity, no residue, no pollution and no public nuisance, and is particularly beneficial to environmental protection.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to application of a compound magnolol as an active ingredient in preparation of an agricultural bactericide.
Background
Diseases of crops are not easy to be detected by people in time like pests and weeds, so that neglect and difficulty in prevention and control are caused, and the damage is more serious. It is reported that plant diseases caused by pathogenic fungi alone account for as many as ten thousand in the world, and the loss caused accounts for 10% -30% of the annual total loss of crops. If plant diseases caused by viruses, bacteria, nematodes and the like are counted, the loss is more remarkable.
In order to meet the increasing demand for foodstuffs and other living materials, chemicals have to be used to protect crops from attack by harmful organisms, of which fungicides play an important role. Natural products with agricultural bactericidal activity are generally easy to be absorbed and conducted by plants, and have little or no phytotoxicity to plants, so that the natural products are paid much attention.
Magnolol, english name: magnol, english name: 2,2' -Bichavicol, CAS number: 528-43-8, molecular formula: C 18 H 18 O 2 Molecular weight 266.34, molecular structure:
the compound is dissolved in organic solvent such as methanol, ethanol, acetone, ethyl acetate, chloroform, etc. The research on the activity of the compound for inhibiting the phytopathogen is less.
Disclosure of Invention
The invention aims to explore the application of a compound magnolol as an active ingredient in preparing agricultural fungicides.
A large number of researches prove that the agricultural bactericide prepared by taking the compound as an active ingredient has excellent bactericidal activity and wide bacteriostatic spectrum, and can be applied to preventing and treating fungal diseases on various crops, such as: sclerotinia rot, banded sclerotial blight, gray mold, gibberellic disease, anthracnose, bakanae disease, gummy stem blight, fusarium wilt and verticillium wilt.
The compound magnolol can be obtained by separating and purifying natural plants, or by chemical synthesis. The compound can be used as an active ingredient, a pesticide auxiliary agent and an excipient to be prepared into any formulation allowed in pesticides. It can also be compounded with other pesticides, and mixed with pesticide adjuvant and excipient to make any dosage form allowable on pesticides. The preparation formulation can be missible oil, a suspending agent, an aqueous emulsion, wettable powder, a microemulsion, water dispersible granules and the like. The used auxiliary agent can adopt calcium dodecyl benzene sulfonate, pyrrolidone, polyoxyethylene polyoxypropylene block compound, nonylphenol polyoxyethylene ether and the like, and the excipient can be cyclohexanone, solvent oil, water, propylene glycol, deionized water and the like. Other pesticides used for compounding can be organic bactericides, such as triazole bactericides triadimenol; botanical bactericides such as allicin, osthole, etc.
Compared with the prior art, the invention has the beneficial effects that: the compound magnolol is used as a bactericide, has a wide antibacterial spectrum and a long persistent period, and is an ideal bactericide in agriculture. Compared with common chemical pesticides, the plant extract is derived from natural plants, has the characteristics of low toxicity, no residue, no pollution and no public nuisance for various economic crops such as grains, melons, fruits, vegetables and the like, and is more beneficial to environmental protection.
Detailed Description
The effects of the present invention are further illustrated by the following specific embodiments:
example 1: prevention and treatment of agricultural diseases by compound magnolol
Test strains Rhizoctonia solani (A)Thanatephorus cucumeris)Separating from rice sheath blight disease leaves; sclerotinia sclerotiorum (A) and (B)Sclerotioia selerotiorum) Separating from sclerotinia rot of colza strain; botrytis cinerea (A. Cinerea)Botrytis cinerea) Separating from strawberry gray mold; fusarium graminearum (F.), (Fusarium graminearum) Separating from wheat scab; colletotrichum gloeosporioides (B)Colletotrichum gloeosporioides) Separating from grape anthracnose disease plants; fusarium moniliforme (F.), (Fusarium moniliforme) Is separated from the rice bakanae disease strain. The six strains are provided by the plant protection institute of Nanjing university of agriculture. Shell fungus of black rot melon (Didymella bryoniae) Is separated from a watermelon gummy stem blight disease plant and is provided by plant research institute of Chinese academy of sciences of Jiangsu province. Fusarium oxysporum (F.), (Fusarium oxysporum) Verticillium dahliae separated from cotton wilt disease plants: (Verticillium dahliae) Is separated from cotton verticillium wilt disease plants and is provided by the plant protection of agricultural academy of Jiangsu province.
The test compound magnolol is dissolved in DMSO to prepare a mother solution of 1000 mug/mL, and the mother solution is stored for later use. PDA culture medium, 200g peeled potato is cut into blocks, boiled for 10min, juice is taken, 20g agar is added, heating is carried out until melting, 20g glucose is added, and water is added for constant volume until 1000mL.
The inhibition of hypha growth is determined by hypha growth rate determination, a bacterial dish (diameter 5 mm) is punched from the edge of a test bacterium pre-cultured on a PDA plate, the mother liquor of the test reagent is uniformly mixed with a culture medium, the final concentration of the test reagent is respectively 30, 20, 10, 5, 2.5 and 0ppm, the mixed liquor is respectively poured into a sterilized culture dish with the diameter of 9cm to prepare a plate, each dish is inoculated with a bacterial dish, the hypha face upwards, and each treatment is repeated for 3 times. And (4) culturing at 25 ℃ until the control colonies are more than two thirds of the full culture dish, and measuring the colony growing diameter by a cross method. The growth inhibition rate was calculated from the average colony diameter (mm). The obtained data is analyzed and processed by DPS V3.01 professional version software to obtain a standard curve and EC 50 The value is obtained.
TABLE 1 bacteriostatic rate of the compounds on agricultural diseases
| Plant pathogenic bacteria | Regression equation | R | EC 50 (mg/L) | 95% confidence interval |
| Rhizoctonia solani | y=4.2584+0.8990x | 0.9796 | 6.68 | 5.08~8.79 |
| Sclerotinia sclerotiorum | y=3.4603+1.6299x | 0.9681 | 8.80 | 6.63~11.70 |
| Fusarium graminearum | y=4.1936+0.9652x | 0.9952 | 6.85 | 6.02~7.79 |
| Colletotrichum gloeosporioides | y=3.7011+1.4000x | 0.9841 | 8.47 | 6.91~10.38 |
| Botrytis cinerea | y=2.4670+3.1238x | 0.9763 | 6.47 | 4.06~10.31 |
| Fusarium moniliforme | y=3.6031+1.3527x | 0.9581 | 10.78 | 6.99~16.63 |
| Fusarium oxysporum | y=2.6826+2.0258x | 0.9686 | 13.93 | 10.47~18.53 |
| Melon Eichhornia melanosporum | y=0.9989+3.3072x | 0.9822 | 16.21 | 13.42~19.59 |
| Verticillium dahliae | y=1.9460+2.4025x | 0.9736 | 18.67 | 14.55~23.96 |
The results show that the compound magnolol can effectively prevent and treat bacteriaNuclear disease, banded sclerotial blight, gray mold, gibberellic disease, anthracnose, bakanae disease, gummy stem blight, fusarium wilt and verticillium wilt, and the moderate concentration EC for inhibiting the diseases 50 6.47-18.67 ppm, and the specific experimental results are shown in Table 1. The obvious effect of the pesticide is fully embodied through experiments.
Example 2: weighing the compound and conventional auxiliary materials such as lignin, diatomite and the like, and preparing the wettable powder with the effective component concentration of 2% according to a conventional process.
Example 3: the compound is weighed and mixed with conventional auxiliary materials such as calcium dodecyl benzene sulfonate, phenethyl phenol polyoxyethylene ether, solvent oil and the like according to a conventional process to prepare missible oil with the concentration of the effective component of 2 percent.
Example 4: the compound and conventional auxiliary materials such as sodium lignosulphonate, kaolin and the like are weighed and prepared into water dispersible granules with the effective component concentration of 2% according to a conventional process.
Example 5: weighing the compound, solvent oil, pyrrolidone, xanthan gum and other conventional auxiliary materials, and preparing the mixture into an aqueous emulsion with the concentration of an effective component of 1% according to a conventional process.
Example 6: weighing the compound and conventional auxiliary materials such as calcium dodecyl benzene sulfonate, pyrrolidone and the like, and preparing the microemulsion with the concentration of the effective component of 1% according to the conventional process.
Example 7: according to the weight ratio of 1:5, weighing the compound and the triadimenol, and preparing the compound and the triadimenol with conventional auxiliary materials such as solvent oil, pyrrolidone, xanthan gum and the like into the aqueous emulsion with the effective component concentration of 5 percent according to a conventional process.
Example 8: according to the weight ratio of 6:1, weighing the compound and the allicin, and preparing the compound and the allicin with conventional auxiliary materials such as solvent oil, pyrrolidone, xanthan gum and the like into an aqueous emulsion with the concentration of an effective component of 5 percent according to a conventional process.
Example 9: according to the weight ratio of 5:1, weighing the compound and osthole, and preparing the compound and osthole into an aqueous emulsion with the concentration of 5 percent of effective components with conventional auxiliary materials such as solvent oil, pyrrolidone, xanthan gum and the like according to a conventional process.
Claims (3)
1. Magnolol as active ingredient for preparing water melonThe application of the bactericide for gummy stem blight is characterized in that the pathogen of gummy stem blight is cucurbitaceous nigrosporaDidymella bryoniae。
2. The use according to claim 1, wherein the active ingredient and the pesticide adjuvant are mixed to make any formulation allowed on pesticides; or the active ingredient is mixed with other pesticides and pesticide auxiliary agents to prepare any formulation allowed on pesticides.
3. Use according to claim 2, characterized in that the formulation is an emulsifiable concentrate, a suspension, an aqueous emulsion, a wettable powder, a microemulsion or a water dispersible granule; the other pesticides are triazole fungicide triadimenol or botanical fungicides allicin and osthole.
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| CN114209678B (en) * | 2021-12-24 | 2023-05-02 | 北京市农林科学院 | Application of magnolol in relieving fumonisin B1 induced cytotoxicity |
| CN116138255B (en) * | 2023-01-09 | 2024-09-17 | 河南科技学院 | Biopesticide composition for preventing and treating wheat stripe rust |
| CN116616286B (en) * | 2023-05-25 | 2024-06-21 | 河南科技学院 | Pesticide composition for preventing and treating banded sclerotial blight of wheat |
| CN118415175B (en) * | 2024-07-03 | 2024-09-06 | 云南省农业科学院药用植物研究所 | Method for preventing and treating rhizome rot of paris polyphylla |
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| CN102369956A (en) * | 2011-10-18 | 2012-03-14 | 浙江农林大学 | Plant essential oil containing agricultural fungicide and sterilization applications thereof |
| CN103503928A (en) * | 2013-01-24 | 2014-01-15 | 杜新明 | Biological insecticidal and bactericidal agent |
| CN103961285A (en) * | 2014-05-21 | 2014-08-06 | 韩一波 | Formula of plant active liquid shampoo and preparation method thereof |
| CN107691480A (en) * | 2017-08-30 | 2018-02-16 | 北京联合大学 | A kind of magnolol synergy fungicide for being used to prevent and treat ring spot |
| CN110583766A (en) * | 2019-10-29 | 2019-12-20 | 浙江黛君生物医药科技有限公司 | Fruit preservative and preparation method thereof, fruit preservation method and application |
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