CN111296439B - Magnolia officinalis phenol bactericide and preparation method and application thereof - Google Patents
Magnolia officinalis phenol bactericide and preparation method and application thereof Download PDFInfo
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- CN111296439B CN111296439B CN202010266342.3A CN202010266342A CN111296439B CN 111296439 B CN111296439 B CN 111296439B CN 202010266342 A CN202010266342 A CN 202010266342A CN 111296439 B CN111296439 B CN 111296439B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention belongs to the field of crop disease control, and discloses a magnolol bactericide and application thereof. The bactericide comprises the following components in percentage by weight: 0.5-30% of active ingredient, 5-10% of cosolvent, 10-15% of emulsifier, 1-5% of antifreezing agent and the balance of solvent. Wherein the active ingredient is selected from one or more of magnolol, honokiol, and honokiol. The bactericide prepared by the invention has good control effects on tea anthracnose, tea round spot, tea brown bud disease, tea white star disease and tea cake disease. The preparation can prevent and treat tea tree diseases by soaking roots of tea seedlings, spraying stems and leaves of tea trees, irrigating tea tree planting fields and the like, and has the advantages of safety, environmental protection and high efficiency.
Description
Technical Field
The invention belongs to the field of crop disease control, relates to a magnolol bactericide and application of the bactericide in tea tree disease control, and particularly relates to application of a bactericide containing magnolol, honokiol and/or honokiol in tea tree disease control.
Technical Field
Tea trees are perennial economic crops, and after the tea industry of China enters an intensification stage in recent decades, the planting area of a tea garden is rapidly enlarged, the trend of a tea area ecological system is simplified, the tea garden is low in biological diversity, a food chain is simple, ecological functions and components are simplified, and the outbreak of plant diseases and insect pests is easily caused. 72 kinds of tea tree fungal diseases are recorded in China. Only take tea anthracnose as an example, the outbreak of tea anthracnose in a tea garden in Fuding county in 1991 causes the number of diseased leaves to be nearly 4000 leaves/m2The yield of the tea leaves is reduced by 25-30%, the serious yield reduction rate is 50%, the average yield reduction of the tea leaves in the next year is 20%, and hundreds of millions of tea seedlings are damaged.
At present, the main prevention and treatment means of tea tree diseases still is chemical pesticide. Although some problems are solved by chemical pesticides, the chemical pesticides have long residual time, are harmful to human and livestock and have destructive influence on the environment. In addition, resistance of tea plant pathogens to chemical fungicides is also an important issue. At present, the natural products have been researched and reported to prevent and treat the tea tree diseases, but the magnolol compounds have not been reported to prevent and treat the tea tree diseases.
The technical problem to be solved by the invention is as follows: the natural product bactericide and the production method of the preparation thereof are provided for preventing and treating the fungal diseases of tea trees, the natural product magnolol, honokiol and honokiol prepared by the method are taken as main raw materials and are scientifically prepared, and the pesticide is nontoxic and harmless to human and livestock, is environment-friendly, can replace the currently used chemical and chemical synthetic pesticides, and can meet the sterilization requirements of crops such as tea leaves and the like.
Disclosure of Invention
The invention aims to provide a bactericide which is efficient and safe, has no pesticide residue and can prevent and treat tea tree diseases, a preparation method thereof and application thereof in preventing and treating tea tree diseases.
In order to achieve the purpose, the invention adopts the following technical scheme:
a magnolol bactericide comprises an active ingredient, wherein the active ingredient comprises one or more of magnolol, honokiol and honokiol.
Furthermore, the bactericide also comprises a cosolvent, an emulsifier, an antifreezing agent and a solvent.
Further, the active ingredient accounts for 0.5 to 30 percent of the mass of the bactericide; the mass fraction of the cosolvent in the bactericide is 5-10%, the mass fraction of the emulsifier in the bactericide is 10-15%, the mass fraction of the antifreeze in the bactericide is 1-5%, and the balance is solvent.
Further, the cosolvent is one or more of ethylene glycol, isopropanol, cyclohexanone, N-methyl pyrrolidone and sodium salicylate.
Further, the emulsifier is one or more of alkylphenol ethoxylates, polyol fatty acid ester and ethylene oxide adduct thereof, sulfonate, fatty alcohol polyoxyethylene ether and tea saponin.
Further, the antifreezing agent is one or more of propylene glycol, glycerol and polyethylene glycol; the solvent is ethanol.
The invention also discloses a method for preparing any of the magnolol fungicide, which comprises the following steps:
adding the active ingredients into the cosolvent and a part of the solvent, stirring until the active ingredients are completely dissolved, adding the emulsifier, uniformly stirring, adding the antifreezing agent, and finally adding the rest solvent, and fully and uniformly stirring to obtain the magnolol bactericide.
The invention also discloses the magnolol bactericide prepared by the preparation method, which comprises active ingredients, wherein the active ingredients comprise one or more of magnolol, honokiol and honokiol.
Use of a magnolol fungicide according to any one of the preceding claims for the control of tea plant diseases including, but not limited to, tea anthracnose, tea round spot, tea brown bud disease, tea star disease, tea cake disease.
The application of the magnolol fungicide prepared according to the preparation method in controlling tea plant diseases including but not limited to tea anthracnose, tea round spot, tea brown bud disease, tea white star disease and tea cake disease.
Further, the application method of the magnolol bactericide comprises the following steps: mixing magnolol bactericide and pesticide, soaking tea seedling root, spraying tea stem and leaf, and irrigating tea plant.
Compared with the prior art, the invention has the following advantages:
1. the bactericide produced by the invention has good effect of preventing and treating tea tree diseases, and the preparation is mixed with other pesticides for preventing and treating the bactericide of the tea tree diseases, mainly preventing and treating tea anthracnose, tea round spot, tea brown bud disease, tea white star disease and tea cake disease; the preparation can be applied by conventional sprayer spray, root soaking and root irrigation. The test results in tables 4-8 show that the invention has obvious control effects on tea anthracnose, tea leaf spot, tea brown bud disease, tea leaf spot and tea cake disease, and the lasting period of the preparation reaches more than 14 days.
2. The field control effect of the preparation on tea anthracnose is mostly more than 70 percent; the best field prevention rate of tea wheel spot disease exceeds 80 percent; the best field control effect on the tea white spot reaches 69 percent; the best field control effect on the tea brown bud disease reaches 89%; the best field prevention rate for the tea cake diseases reaches 82 percent; the control effect is far better than that of the chemical pesticide commonly used in the control tea garden.
3. The preparation has the advantages of safe formulation, low toxicity and environmental protection. Magnolol, honokiol and honokiol, the main chemically active ingredients of the formulations of the present invention are plant secondary metabolites that are easily degraded in the environment and used in modern clinical practice in asia, while in the us and europe they can be used as cosmetic additives, metabolized by the hepatic pathway to produce glucuronic acid derivatives, which are generally considered harmless to humans and animals.
Detailed Description
Example 1
Preparation of 5% magnolol, honokiol and/or honokiol formulation:
in this example, the preparation of 8 5% magnolol (HPF) formulations is described in detail, with the contents of the respective target components shown in Table 1.
TABLE 1.8 content (%)% of the components of the 5% HPF formulations
According to the results in table 1, in the HPF nos. 1-8, the cosolvent is sodium salicylate and N-methylpyrrolidone (mass ratio is 3: 2), the emulsifier is alkylphenol ethoxylates, sulfonate and tea saponin (mass ratio is 1: 2), and the antifreeze is propylene glycol and polyethylene glycol (mass ratio is 1: 1). The solvents of the HPF No. 1-8 preparations are all ethanol.
The preparation of the formulations No. 1 to 8 of example 1 is as follows: weighing magnolol, honokiol and/or the total amount of honokiol (0.5kg) according to the proportion, adding 0.5kg cosolvent and 5kg solvent, stirring until the solvent is completely dissolved, adding 1kg emulsifier, stirring uniformly, adding 0.2kg antifreeze, adding the rest solvent, and stirring uniformly to obtain 10kg HPF 1-8 preparation.
Example 2
Preparation of 15% magnolol, honokiol and/or honokiol formulation:
in this example, the preparation of 7 15% magnolol (HPF) formulations is described in detail, with the contents of the respective target components shown in Table 2.
TABLE 2.7 contents (%)% of the components of the 15% HPF formulations
According to the results in table 2, in the formulation No. 9-15, the cosolvent is sodium salicylate and N-methyl pyrrolidone (mass ratio is 3: 2), the emulsifier is alkylphenol ethoxylate, sulfonate and tea saponin (mass ratio is 1: 2), and the antifreeze is propylene glycol and polyethylene glycol (mass ratio is 1: 1). The solvents of the HPF No. 9-15 preparation are all ethanol.
The preparation of formulation nos. 9 to 15 in example 2 was as follows: weighing magnolol, honokiol and/or honokiol according to a ratio (1.5kg), adding 0.5kg cosolvent and 5kg solvent, stirring until the solvent is completely dissolved, adding 1kg emulsifier, stirring uniformly, adding 0.2kg antifreeze, and finally adding the rest solvent, and stirring uniformly to obtain 10kg HPF 9-15 preparation.
Example 3
Preparation of 30% magnolol, honokiol and/or honokiol formulation:
in this example, the preparation of 7 30% HPF formulations is described in detail, with the contents of the respective target components as shown in table 3.
TABLE 3.7 contents (%) -of the constituents of the 30% HPF preparations
According to the results in Table 3, it can be seen that the cosolvent in the HPF16-22 preparation is sodium salicylate and N-methylpyrrolidone (mass ratio is 4: 1), the emulsifier is alkylphenol ethoxylates, sulfonate and tea saponin (mass ratio is 1: 1), and the antifreeze is propylene glycol and polyethylene glycol (mass ratio is 1: 2). The solvent of HPF16-22 preparation is ethanol.
The preparation of formulation nos. 16 to 22 in example 3 was as follows: weighing magnolol, honokiol and/or honokiol according to a ratio (3kg), adding 0.5kg cosolvent and 5kg solvent, stirring until the solvent is completely dissolved, adding 1kg emulsifier, stirring uniformly, adding 0.2kg antifreeze, adding the rest solvent, and stirring uniformly to obtain 10kg HPF16-22 preparation.
Example 4
Test of prevention effect of the preparation of the invention on related tea tree diseases
The invention has the effect of preventing and treating the anthracnose of tea
(1) Test agents and concentrations thereof: the total concentration of the HPF1 to 8 preparations is 100mg/L for testing magnolol, honokiol and/or honokiol;
(2) the test method comprises the following steps: the method is implemented when anthracnose is flourishing on tea trees. Each treatment is provided with 4 repetitions, the cell area is 20m2And (3) randomly arranging the medicines in blocks, adding water according to the test concentration of each medicine, spraying by a manual sprayer, taking spray clear water as a control, examining the control effect 7 days and 14 days after the medicine is taken before the medicine, and carrying out difference significance analysis (New Duncan's double range method).
(3) The control effect is as follows: the control results for anthracnose on tea trees are shown in table 4.
TABLE 4 prevention of anthracnose of tea with the preparation
From the results in table 4, it can be seen that: the preparation disclosed by the invention has an obvious control effect on the tea anthracnose, the lasting period is longer than 14 days, most of the field control effects of the preparation disclosed by the embodiment 1 on the tea anthracnose are more than 70%, the control effect is far higher than that of chlorothalonil, and the control effect and the difference are obvious.
Example 5
Test of preventive effect on tea leaf spot
(1) Test agents and concentrations thereof: the total concentration of 7 HPFs 9 to 15 of the invention is 100mg/L for testing magnolol, honokiol and/or honokiol;
(2) the test method comprises the following steps: the method is implemented when the tea plant has strong alternaria leaf spot. Each treatment is provided with 4 repetitions, the cell area is 20m2And (3) randomly arranging the medicines in blocks, adding water according to the test concentration of each medicine, spraying by a manual sprayer, taking spray clear water as a control, examining the control effect 7 days and 14 days after the medicine is taken before the medicine, and carrying out difference significance analysis (New Duncan's double range method).
(3) The control effect is as follows: the control results for tea leaf spot are shown in table 5.
TABLE 5 prevention of tea leaf spot by the formulations
From the results in table 5, it can be seen that: the preparation has obvious control effect on tea leaf spot, the lasting period is more than 14 days, the best field control rate of the preparation in the example 2 on the tea leaf spot is more than 80%, and compared with the common chemical pesticide benomyl, the preparation has obvious control effect difference which is far higher than that of the benomyl.
Example 6
The invention is used for the prevention and treatment of the tea leaf spot
(1) Test agents and concentrations thereof: the total concentration of 7 HPF16-22 preparations of the invention is 100mg/L for testing magnolol, honokiol and/or honokiol;
(2) the test method comprises the following steps: it is applied when the white-star disease is profuse. Each treatment is provided with 4 repetitions, the cell area is 20m2And (3) randomly arranging the medicines in blocks, adding water according to the test concentration of each medicine, spraying by a manual sprayer, taking spray clear water as a control, examining the control effect 7 days and 14 days after the medicine is taken before the medicine, and carrying out difference significance analysis (New Duncan's double range method).
(3) The control effect is as follows: the control results of the leaf scald disease on tea trees are shown in table 6.
TABLE 6 prevention of tea leaf spot by the preparation
From the results in table 6, it can be seen that: the preparation has obvious control effect on the tea leaf spot, the lasting period is more than 14 days, the best field control rate of the preparation in the embodiment 3 on the tea leaf spot reaches 69%, and compared with the common chemical pesticide thiophanate-methyl, the preparation has obvious control effect difference which is far higher than that of the thiophanate-methyl.
Example 7
The invention provides a test for the prevention effect of tea brown bud disease
(1) Test agents and concentrations thereof: the total concentration of 7 HPF16-22 preparations of the invention is 100mg/L for testing magnolol, honokiol and/or honokiol;
(2) the test method comprises the following steps: it is applied when the tea brown bud disease is developed. Each treatment is provided with 4 repetitions, the cell area is 20m2And (3) randomly arranging the medicines in blocks, adding water according to the test concentration of each medicine, spraying by a manual sprayer, taking spray clear water as a control, examining the control effect 7 days and 14 days after the medicine is taken before the medicine, and carrying out difference significance analysis (New Duncan's double range method).
(3) The control effect is as follows: the control results for brown bud disease are shown in table 7.
TABLE 7 preventive effect of formulations on tea brown bud disease
From the results in table 7, it can be seen that: the preparation has obvious prevention and treatment effects on the tea brown bud disease, the lasting period is longer than 14 days, the best field prevention and treatment rate of the preparation in the embodiment 4 on the tea brown bud disease reaches 89%, and compared with a common chemical pesticide carbendazim, the preparation has obvious prevention and treatment effects, and is far higher than the carbendazim.
Example 8
The invention is used for the prevention and treatment of tea cake diseases
(1) Test agents and concentrations thereof: in total, 7 preparations are prepared from 1 to 7 of the invention, and the total concentration of the tested magnolol, honokiol and/or honokiol is 100 mg/L;
(2) the test method comprises the following steps: the tea cake is applied when the tea cake on the tea plant is frequently suffered from diseases. Each treatment is provided with 4 repetitions, the cell area is 20m2And (3) randomly arranging the medicines in blocks, adding water according to the test concentration of each medicine, spraying by a manual sprayer, taking spray clear water as a control, examining the control effect 7 days and 14 days after the medicine is taken before the medicine, and carrying out difference significance analysis (New Duncan's double range method).
(3) The control effect is as follows: the control results for tea cake disease are shown in table 8.
TABLE 8 prevention of tea cake disease by formulations
From the results in table 8, it can be seen that: the preparation has obvious control effect on tea cake diseases, the lasting period is longer than 14 days, the best field control effect of the preparation in example 5 on the tea cake diseases reaches 82%, and compared with the common chemical pesticide Bordeaux mixture, the preparation has obvious control effect difference which is far higher than that of the Bordeaux mixture.
Claims (8)
1. The application of the magnolol fungicide in the prevention and treatment of tea plant diseases is characterized in that the diseases comprise tea anthracnose, tea round spot, tea brown bud disease, tea leaf spot and tea cake disease; the active ingredient in the magnolol bactericide is composed of one or more of magnolol, honokiol and honokiol.
2. The use of claim 1, wherein the bactericide further comprises a cosolvent, an emulsifier, an antifreeze, and a solvent.
3. The use as claimed in claim 2, wherein the active ingredient is present in an amount of 0.5 to 30% by weight of the magnolol-type fungicide; the cosolvent accounts for 5-10% of the magnolol bactericide, the emulsifier accounts for 10-15% of the magnolol bactericide, the antifreezing agent accounts for 1-5% of the magnolol bactericide, and the balance is a solvent.
4. Use according to claim 3, wherein the co-solvent is one or more of ethylene glycol, isopropanol, cyclohexanone, N-methylpyrrolidone and sodium salicylate.
5. The use of claim 3, wherein the emulsifier is one or more of alkylphenol ethoxylates, polyol fatty acid esters and ethylene oxide adducts thereof, sulfonates, fatty alcohol ethoxylates and tea saponin.
6. The use according to claim 3, wherein the anti-freezing agent is one or more of propylene glycol, glycerol and polyethylene glycol; the solvent is ethanol.
7. The use as claimed in claim 3, wherein the method of preparation of the magnolol fungicide comprises the steps of: adding the active ingredients into the cosolvent and a part of the solvent, stirring until the active ingredients are completely dissolved, adding the emulsifier, uniformly stirring, adding the antifreezing agent, and finally adding the rest solvent, and fully and uniformly stirring to obtain the magnolol bactericide.
8. Use according to any one of claims 1 to 7, wherein the magnolol fungicide is used by spraying, root soaking and root drenching.
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112120024A (en) * | 2020-10-09 | 2020-12-25 | 青岛农业大学 | Application of honokiol in preparation of bactericide for preventing and treating plant diseases caused by downy mildew |
| CN112438260B (en) * | 2020-12-08 | 2022-06-24 | 中国农业科学院烟草研究所 | Application of honokiol in preparation of natural environment-friendly pesticide |
| CN113170788B (en) * | 2021-04-26 | 2022-04-26 | 江苏省中国科学院植物研究所 | Application of honokiol as agricultural bactericide |
| CN113207878B (en) * | 2021-04-26 | 2022-11-29 | 江苏省中国科学院植物研究所 | Application of magnolol as agricultural bactericide |
| CN116138255B (en) * | 2023-01-09 | 2024-09-17 | 河南科技学院 | Biopesticide composition for preventing and treating wheat stripe rust |
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