CN106234442A - A kind of Fructus Litchi antibacterial and preparation method thereof - Google Patents
A kind of Fructus Litchi antibacterial and preparation method thereof Download PDFInfo
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- CN106234442A CN106234442A CN201610650586.5A CN201610650586A CN106234442A CN 106234442 A CN106234442 A CN 106234442A CN 201610650586 A CN201610650586 A CN 201610650586A CN 106234442 A CN106234442 A CN 106234442A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
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Abstract
The present invention relates to technical field of pesticide, disclose a kind of Fructus Litchi antibacterial and preparation method thereof.This antibacterial is made up of the raw material of following parts by weight: Radix Mirabilis extract 12 22 parts, Mancozeb 5 12 parts, Eupatorium cannabinum L. (Eupatorium coelestinum L.) 5 10 parts, 39 parts of carbonic acid, 27 parts of Pericarpium Zanthoxyli, solvent 15 35 parts.Fructus Litchi antibacterial of the present invention has low toxicity, environmental protection, and Fructus Litchi fungicidal spectrum is wide, and the lasting period is long, effectively slows down drug-fast advantage, and is comprehensively utilized by invasive species, turns waste into wealth, and is to utilize in disease on agricultural production preventing and treating Fructus Litchi.
Description
Technical field
The present invention relates to technical field of pesticide, particularly relate to a kind of Fructus Litchi antibacterial and preparation method thereof.
Background technology
Fructus Litchi (formal name used at school:Litchi chinensis Sonn.) Sapindaceae, Fructus Litchi belongs to aiphyllium, high
About 10 meters.Peel has fish-scale shape projection, scarlet, purplish red.Ripe up to cerise；Seed is all wrapped up by meat aril.Flower
Spring phase, really summer phase.Translucent lipoid shape when sarcocarp produces fresh, taste perfume (or spice) is beautiful, but not storage endurance.Be distributed in China the west and south,
South and the southeast, Guangdong and south Fujian cultivation are contained most.Also there is cultivation the southeast, Asia, and Africa, America and Oceania have draws
The record planted.Fructus Litchi and Fructus Musae, Fructus Ananadis comosi, Arillus Longan are together known as " the big fruit in the southern part of the country four ".Fructus Litchi sweet in the mouth, acid, warm in nature, enter the heart,
Spleen, Liver Channel；Can stop singultus, only diarrhoea, is the dietetic therapy good merchantable brand of intractable singultus and diarrhea before dawn person, has supplementing the brain body-building simultaneously, appetizing
Strengthening the spleen, has the effect promoting appetite.Because of property heat, polyphagia is easily got angry.Fructus Litchi timber is solid, and texture is graceful, and corrosion resistant, is always first-class
Name material.
The pest and disease damage of Fructus Litchi is more, affects yield and the quality of Fructus Litchi, hampers the development that Fructus Litchi produces, therefore, Fructus Litchi
The prevention and control of plant diseases, pest control become the upper urgent problem of production.It is currently used for the antibacterial of Fructus Litchi controlling disease, the most anti-
Controlling single disease, prevention effect is the most extensive.
Mirabilis jalapa (Mirabilis jalapa Linnaeus) be commonly called as Radix primulae maximowiczii, snowball, Rhizoma Anemones flaccidae flower,
Four-o'clock flower etc., genus Nyctaginaceae (Nyctaginaceae), for annual herb plant, cold in nature, sweet in the mouth, root,
Stem, leaf, flower, seed all can be used as medicine.There are heat-clearing and toxic substances removing, effect through dredging collateral inducing diuresis and reducing edema, alive.Purple jasmine is proved through pharmacological research
The root of jasmine has anticancer, antipathogenic composition, and leaf has diuresis, catecholamine effect, and seed has contraception, haemolysis and hemostatic composition.So far, portion
Dividing adventitious plant the most worldwide to cause serious disaster, agricultural and other related industry are not only caused danger greatly by it
Evil, and ecological environment especially bio-diversity in native country is constituted serious threat, its potential negative effect is also difficult to estimate
Amount.And Mirabilis jalapa introduces China and has shown obvious wildness or invasive.
In recent years, due to the people's unreasonable use to chemical pesticide and the inherent defect such as fungicidal spectrum of medicament itself thereof
Single, it is used for multiple times and is easily generated Drug resistance, medicine component toxicity is the strongest, and it is a series of that these make pesticide in use create
Pollution problem, such as environmental pollution, person poultry poisoning, killing natural enemy, destroy ecological balance, " 3R " (Residue, Resistance,
The serious problems such as Resurgence), these harmful effects have caused the common concern of people.In recent years on pesticide market, domestic
Antibacterial is sold and is glided always, mainly can not show a candle to the preventive effect of external antibacterial because of its preventive effect controlling disease.And develop in China
A kind of novel pesticide composition, Meteorological is high, and technology is immature.Though external novel pesticide composition drug effect is good, but price is high.Therefore,
It is to develop pesticide new product at present and prevent and treat the one of resistance disease that the pesticide active ingredient utilizing the different mechanism of action carries out compounding
Plant effective quick way.Different pesticide active ingredients carry out compounding after, three kinds of type of action can be shown: summation action, increasing
Effect effect and antagonism.Generally, only by substantial amounts of test just can learn different pesticide active ingredient compounding after effect
Type.
Although the antibacterial containing Mirabilis jalapa composition being studied at present and achieves some achievements, but great majority only all existing
Being that indivedual a few class diseases are carried out effect experiment, range is limited, and has only focused on preventive effect, whether produces its Drug resistance
Raw, use the problems such as harm that biological invasion itself had how alleviation not make and further study.Such as China
Patent CN201410027118.3 mono-kind prevents and treats the Radix Mirabilis extract preparation method and applications of Agricultural Mites, send elsewhere bright very
A kind of efficient miticide is prepared in the effect of the repellent parasite killing being had in the Radix Mirabilis extract of good utilization, to a certain degree
On serve the effect of preventing and treating Agricultural Mites, but the preventive effect of acaricide itself is simply illustrated by this patent of invention, its
Not relevant experiment of whether developing immunity to drugs after use proves, it is difficult to ensure that the holding effect of medicament, in addition purple jasmine
Jasmine is the instruction plant that a class hazard ratio is more serious, for this kind of for Mirabilis jalapa instruction plant is comprehensive as the main component of pesticide
Utilizing, this mode with great economic benefit and social benefit that turns waste into wealth seldom is having been reported that.And for litchi
The subject matter that the research of branch antibacterial presently, there are is exactly that broad spectrum activity is poor, such as Chinese patent CN201510774022.8 mono-
Planting Fructus Litchi pesticide of anti-sour rot and preparation method thereof, this patent is for other disease on Fructus Litchi such as Fructus Litchi anthrax, Fructus Litchi
The diseases controls such as powdery mildew, Fructus Litchi graywall, Fructus Litchi shot hole, Fructus Litchi algae pinta are unknown, therefore wide for research Fructus Litchi disease
Compose, slow down the useful direction that drug-fast antibacterial is the research and development of current industry.
Summary of the invention
It is an object of the invention to: for the problem of above-mentioned existence, it is provided that a kind of preventive effect phase is long, and Fructus Litchi fungicidal spectrum is wide, low
Poison, can effectively slow down the generation of disease drug resistance, and the Fructus Litchi antibacterial of pollution reducing environment and preparation method thereof.
In order to realize foregoing invention purpose, the technical solution used in the present invention is as follows:
A kind of Fructus Litchi antibacterial, is made up of the raw material of following parts by weight: Radix Mirabilis extract 12-22 part, Mancozeb 5-12
Part, Eupatorium cannabinum L. (Eupatorium coelestinum L.) 5-10 part, carbonic acid 3-9 part, Pericarpium Zanthoxyli 2-7 part, solvent 15-35 part.
Further, Fructus Litchi antibacterial of the present invention, it is made up of the raw material of following parts by weight: Radix Mirabilis extract 15-20
Part, Mancozeb 7-10 part, Eupatorium cannabinum L. (Eupatorium coelestinum L.) 6-8 part, carbonic acid 5-7 part, Pericarpium Zanthoxyli 4-6 part, solvent 24-31 part.
Further, Fructus Litchi antibacterial of the present invention, it is made up of the raw material of following parts by weight: Radix Mirabilis extract 18 parts,
Mancozeb 8 parts, Eupatorium cannabinum L. (Eupatorium coelestinum L.) 7 parts, 6 parts of carbonic acid, 5 parts of Pericarpium Zanthoxyli, solvent 28 parts.
Further, described solvent be Oleum Arachidis hypogaeae semen, Oleum Brassicae campestris, soybean oil, Oleum sesami, Petiolus Trachycarpi oil, olive oil, Semen Maydis oil and
One or more in mineral oil, preferably Oleum sesami.
Present invention also offers the preparation method of above-mentioned Fructus Litchi antibacterial, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Mirabilis jalapa, wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48 h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Radix Mirabilis extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Radix Mirabilis extract serosity of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Radix Mirabilis extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Eupatorium cannabinum L. (Eupatorium coelestinum L.) and Pericarpium Zanthoxyli are crushed to 3-5mm with plant pulverizer is minced, coarse powder is minced
Proportionally it is mixed and added into solvent with Mancozeb and carbonic acid and Radix Mirabilis extract that step (2) gained has been centrifuged is carried out
Dissolve, mix homogeneously, then grind 20-40 minute with colloid mill, continue on colloid mill, grind 20-40 after adding antifreezing agent and divide
Clock, obtains Fructus Litchi antibacterial colloid；
(4) stirring: Fructus Litchi antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi antibacterial
The 3/4 of colloid full dose, stirs under the rotating speed of 2000-18000 rev/min 20-40 minute, i.e. obtains Fructus Litchi antibacterial water and milk
Agent.
Further, in step (4), described stirring means is as follows: move in emulsifying kettle by Fructus Litchi antibacterial colloid,
Add distilled water to the 3/4 of Fructus Litchi antibacterial colloid full dose, stir 30 minutes under the rotating speed of 8000 revs/min, i.e. obtain Fructus Litchi
Disinfectant aqueous emulsion.
In described raw material, each component brief introduction is as follows:
Mirabilis jalapa (Mirabilis jalapa Linnaeus) it is commonly called as Radix primulae maximowiczii, snowball, Rhizoma Anemones flaccidae flower, careless jasmine
Jasmine etc., genus Nyctaginaceae (Nyctaginaceae).The root of Mirabilis jalapa has anticancer, antipathogenic composition, leaf have diuresis,
Catecholamine effect, seed has contraception, haemolysis and hemostatic composition.Part adventitious plant is the most worldwide caused seriously at present
Disaster, agricultural and other related industry not only cause greatly harm by it, and the most biological to native country ecological environment
Multiformity constitutes serious threat, and its potential negative effect is also difficult to estimate.And Mirabilis jalapa introduces China's the most table
Obvious wildness or invasive are revealed.Carry after the use compounding with Mancozeb of Mirabilis jalapa albumen in Radix Mirabilis extract
Bactericidal activity under the high temperature of high Mancozeb, because of the inefficacy apt to deteriorate of Mancozeb high temperature, and mixes with Radix Mirabilis extract
This shortcoming can well be overcome afterwards.It is right that the interior absorption that Radix Mirabilis extract can also utilize Mancozeb to be had increases
The model of action of disease, increases further and kills the target of disease, the most only Fructus Litchi anthrax is had preventive effect basis it
On, add the preventive effect to Fructus Litchi diseases such as branch powdery mildew, Fructus Litchi graywall, Fructus Litchi shot hole, Fructus Litchi algae pintas, expand litchi
Branch fungicidal spectrum.
Mancozeb sterling is white powder, and industrial goods are canescence or pale yellow powder, have rotten-egg odour, are insoluble in
Water, insoluble in most of organic solvents, but can be dissolved in pyridine, to light, heat, moist instability, easily decomposites Carbon bisulfide, meets
Alkaline matter or the material such as copper, hydrargyrum the most easily decompose to give off Carbon bisulfide and decreasing effect, and volatility is little.So-called protection, it is simply that for plant
Zn element is provided, in addition to solving the symptom of zinc deficiency, strengthens the ability of opposing disease to plant, thus relatively play sterilization and make
With.Mancozeb is the wide-spectrum bactericide that agriculturally controlling disease is conventional, and remaining alkaline matter is used with and may be produced antagonism
Effect, containing carbonic acid in Fructus Litchi antibacterial of the present invention, its effect is the state that medicament keeps non-alkaline, it is ensured that maneb
The drug effect of zinc can play.It is used alone easily inefficacy under Mancozeb high temperature, with the Mirabilis jalapa egg in Radix Mirabilis extract simultaneously
Improve bactericidal activity after white mixing, overcome the shortcoming can not being used alone under high temperature.
Eupatorium cannabinum L. (Eupatorium coelestinum L.) (Eupatorium adenophora Spreng.) belong to a kind of invasion life
Thing, is constantly subjected to global extensive concern, studies the most always and how can utilize the favourable of Eupatorium cannabinum L. (Eupatorium coelestinum L.)
Composition, can eliminate again its adverse influence.Eupatorium cannabinum L. (Eupatorium coelestinum L.) is not only a kind of spice having Utilization prospects, and also by sending out
Ferment detoxification is made feedstuff, is manufactured fibre board and biogas.Purple stem boneset extract has certain bacteriostatic activity, and in Radix Mirabilis extract
Mirabilis jalapa albumen can speed up the synthesis having antibacterial peptide in purple stem boneset extract, improve bactericidal activity.
Carbonic acid (H2CO3) it is a kind of weak diacid, ionization constant is the least.But also have and think that it is middle strong acid, because of according to
Mineral acid Acidity judges that formula (OH) nROm can determine whether that it is acid similar to phosphoric acid.Under room temperature, normal pressure, carbon dioxide is saturated
It is 5.6, pKa=6.37 that the concentration of solution is about 0.033mol/L, pH.(pKa1=6.38, pKa2=10.33, p represent and take H2CO3
Negative common logarithm.) unsaturated carbonate solution (pure CO2, pressure is 1 atm) pH be about 4, and CO under field conditions (factors)2Content is
0.03%, dissolve pH=5.6 when reaching saturated.Mancozeb the most easily produces antagonism, and add carbonic acid it
After Fructus Litchi antibacterial of the present invention is in the environment of a non-alkaline, it is ensured that the performance of Mancozeb drug effect.
Pericarpium Zanthoxyli (Zanthoxylum bungeanum Maxim.), another name:, big green pepper,
Pericarpium Zanthoxyli, another name for Sichuan Province green pepper, Fructus Zanthoxyli or Pericarpium Zanthoxyli.For Rutaceae, Zanthoxylum machaka or dungarunga, can isolated planting can make again to protect thorn hedge.
Pericarpium Zanthoxyli is warm in nature, acrid in the mouth, can play the effect of mediation in Fructus Litchi antibacterial of the present invention, neutralizes medicine Acidity of Aikalinity, prevents medicament inclined
Acid or meta-alkali and Litchi Leaves and fruit are damaged.Extract with the Mirabilis jalapa in seasonal Fructus Litchi microbiocidal composition of the present invention
In thing composition, Mirabilis jalapa albumen solubility improves, and prevents the loss of Fructus Litchi antibacterial effective ingredient.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:
(1) scientific compatibility: a kind of Fructus Litchi antibacterial of the present invention make use of the principle of scientific compatibility, the purple in Radix Mirabilis extract
After the use compounding with Mancozeb of jasmine albumen, improve the bactericidal activity under the high temperature of Mancozeb, overcome Mancozeb
The shortcoming of single use inefficacy apt to deteriorate under high temperature.Radix Mirabilis extract can also utilize the interior absorption that Mancozeb is had
Increase the model of action to disease, increase the target killing disease further, the most only Fructus Litchi anthrax is had preventive effect
On basis, add the preventive effect to Fructus Litchi diseases such as branch powdery mildew, Fructus Litchi graywall, Fructus Litchi shot hole, Fructus Litchi algae pintas, expand
Big Fructus Litchi fungicidal spectrum, in the test of pesticide effectiveness, Fructus Litchi antibacterial of the present invention is average anti-for common Fructus Litchi disease Fructus Litchi anthrax etc.
Effect has reached 85.95%.And Mancozeb the most easily produces antagonism, and make this after adding carbonic acid
Bright Fructus Litchi antibacterial is in the environment of a non-alkaline, it is ensured that the performance of Mancozeb drug effect, in the Drug resistance of the present invention
In experiment, after using first 6 months, preventive effect is averagely reduced only by 1.92%, illustrates that using of Fructus Litchi antibacterial of the present invention will not
Affected by disease drug resistance.Fructus Litchi antibacterial of the present invention utilize the Mirabilis jalapa albumen in Radix Mirabilis extract accelerate purple
In stem Herba Lycopi, the synthesis of Antagonistic protein, improves bactericidal activity.Pericarpium Zanthoxyli can play the effect of mediation, neutralizes medicine Acidity of Aikalinity, anti-
Stop medicament meta-acid or meta-alkali and litchi fruits and blade are damaged.With purple in seasonal Radix Mirabilis extract composition of the present invention
Jasmine albumen solubility improves, and prevents the loss of antibacterial effective ingredient.
(2) low toxicity, environmental protection.Solvent in Fructus Litchi antibacterial of the present invention is soybean oil, and primary raw material source is Mirabilis jalapa,
Natural materials in the natures such as Pericarpium Zanthoxyli, different from chemical pesticide composition conventional on the market, unartificial chemosynthesis, because spraying
Use rear degradable, ecological environment will not be had a negative impact.Although main component purple stem pool in Fructus Litchi antibacterial of the present invention
Orchid has certain toxicity, but possibly together with carbonic acid in present component, carbonic acid has the effect of regulation ph, can neutralize Eupatorium cannabinum L. (Eupatorium coelestinum L.)
The toxicity of alkaloid in composition, improves use safety coefficient.Having is exactly the Pericarpium Zanthoxyli effect that can play mediation again, neutralizes pharmaceutical acid
Alkalescence, pesticide control meta-acid or meta-alkali and to plant leaf produce destroy.Therefore reach to prevent the diffusion of toxicity, control poison
Power, the purpose preserved the ecological environment.
(3) comprehensively utilize, turn waste into wealth: Mirabilis jalapa belongs to instruction plant in China, extensively invade various herbage fields, to agriculture
Animal husbandry produces and causes tremendous economic to lose.But originate using Mirabilis jalapa as primary raw material, on the one hand, utilize and itself had
Bactericidal action, after adding use compounding with Mancozeb, can overcome and under Mancozeb high temperature, be used alone easily change
The shortcoming that matter lost efficacy, has reached potentiation.In the effect experiment of the present invention, Fructus Litchi antibacterial of the present invention is compared in reference examples
Mancozeb, Eupatorium cannabinum L. (Eupatorium coelestinum L.) and Radix Mirabilis extract single dose use it that control rate of disease to improve 29.21%, especially
It is to improve 27.49% than Mancozeb, improves 30.63% than Eupatorium cannabinum L. (Eupatorium coelestinum L.), improve 29.49% than Radix Mirabilis extract.The opposing party
Face, tradition uses physical method or chemical agent to control the invasion danger of Mirabilis jalapa, simply cures the symptoms, not the disease, and utilize purple
Jasmine is as the main component of agriculture chemicals fungicide, by being comprehensively utilized by Mirabilis jalapa, is recycled profit while subduction Mirabilis jalapa
With, produce economic benefit and social benefit so that this biological invasion of Mirabilis jalapa is turned waste into wealth.
(4) Fructus Litchi fungicidal spectrum is wide, the lasting period is long.Mancozeb can be passed through after Mirabilis jalapa use compounding with Mancozeb
The interior absorption being had increases the model of action for Fructus Litchi disease, and then increases the target to Fructus Litchi disease, expands sterilization
Spectrum, on the basis of the most only Fructus Litchi anthrax being killed activity, increases Fructus Litchi powdery mildew, Fructus Litchi graywall, Fructus Litchi skin ulcer
The bactericidal activity of the diseases such as scall, Fructus Litchi algae pinta, in the test of pesticide effectiveness, Fructus Litchi antibacterial of the present invention is for common farmland disease litchi
The average preventive effects such as branch anthrax have reached 85.95%.Mancozeb can also increase Fructus Litchi antibacterial of the present invention to litchi simultaneously
The real adsorptivity with leaves, prevents rain drop erosion, extends the lasting period, in the Drug resistance of the present invention is tested, uses first 6 months
Preventive effect is averagely reduced only by 1.92% afterwards.
Detailed description of the invention
Embodiment 1
A kind of Fructus Litchi antibacterial, is made up of the raw material of following parts by weight: Radix Mirabilis extract 12 parts, Mancozeb 12 parts, purple
Stem Herba Lycopi 5 parts, 9 parts of carbonic acid, 2 parts of Pericarpium Zanthoxyli, solvent 35 parts.
Present invention also offers the preparation method of above-mentioned antibacterial, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Mirabilis jalapa, wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48 h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Radix Mirabilis extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Radix Mirabilis extract serosity of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Radix Mirabilis extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Eupatorium cannabinum L. (Eupatorium coelestinum L.) and Pericarpium Zanthoxyli are crushed to 3mm with plant pulverizer is minced, coarse powder is minced with
The Radix Mirabilis extract that Mancozeb and carbonic acid are proportionally mixed and added into solvent and step (2) gained has been centrifuged carries out molten
Solve, mix homogeneously, then grind 20 minutes with colloid mill, continue after adding antifreezing agent to grind 40 minutes on colloid mill, obtain
Fructus Litchi antibacterial colloid；
(4) stirring: Fructus Litchi antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi antibacterial
The 3/4 of colloid full dose, stirs under the rotating speed of 2000 revs/min 20 minutes, i.e. obtains Fructus Litchi disinfectant aqueous emulsion.
Embodiment 2
A kind of Fructus Litchi antibacterial, is made up of the raw material of following parts by weight: Radix Mirabilis extract 22 parts, Mancozeb 12 parts, purple
Stem Herba Lycopi 5 parts, 9 parts of carbonic acid, 2 parts of Pericarpium Zanthoxyli, solvent 35 parts.
Its preparation method is same as in Example 1.
Embodiment 3
A kind of Fructus Litchi antibacterial, is made up of the raw material of following parts by weight: Radix Mirabilis extract 15 parts, Mancozeb 10 parts, purple
Stem Herba Lycopi 6 parts, 7 parts of carbonic acid, 4 parts of Pericarpium Zanthoxyli, solvent 31 parts.
Present invention also offers the preparation method of above-mentioned antibacterial, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Mirabilis jalapa, wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48 h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Radix Mirabilis extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Radix Mirabilis extract serosity of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Radix Mirabilis extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Eupatorium cannabinum L. (Eupatorium coelestinum L.) and Pericarpium Zanthoxyli are crushed to 5mm with plant pulverizer is minced, coarse powder is minced with
The Radix Mirabilis extract that Mancozeb and carbonic acid are proportionally mixed and added into solvent and step (2) gained has been centrifuged carries out molten
Solve, mix homogeneously, then grind 40 minutes with colloid mill, continue after adding antifreezing agent to grind 20 minutes on colloid mill, obtain
Fructus Litchi antibacterial colloid；
(4) stirring: Fructus Litchi antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi antibacterial
The 3/4 of colloid full dose, stirs under the rotating speed of 15000 revs/min 40 minutes, i.e. obtains Fructus Litchi disinfectant aqueous emulsion.
Embodiment 4
A kind of Fructus Litchi antibacterial, is made up of the raw material of following parts by weight: Radix Mirabilis extract 20 parts, Mancozeb 7 parts, purple stem
Herba Lycopi 8 parts, 5 parts of carbonic acid, 6 parts of Pericarpium Zanthoxyli, solvent 24 parts.
Present invention also offers the preparation method of above-mentioned antibacterial, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Mirabilis jalapa, wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48 h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Radix Mirabilis extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Radix Mirabilis extract serosity of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Radix Mirabilis extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Eupatorium cannabinum L. (Eupatorium coelestinum L.) and Pericarpium Zanthoxyli are crushed to 3.5mm with plant pulverizer is minced, coarse powder is minced
Proportionally it is mixed and added into solvent with Mancozeb and carbonic acid and Radix Mirabilis extract that step (2) gained has been centrifuged is carried out
Dissolve, mix homogeneously, then grind 30 minutes with colloid mill, continue after adding antifreezing agent to grind 35 minutes on colloid mill,
To Fructus Litchi antibacterial colloid；
(4) stirring: Fructus Litchi antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi antibacterial
The 3/4 of colloid full dose, stirs under the rotating speed of 10000 revs/min 25 minutes, i.e. obtains Fructus Litchi disinfectant aqueous emulsion.
Embodiment 5
A kind of Fructus Litchi antibacterial, is made up of the raw material of following parts by weight: Radix Mirabilis extract 18 parts, Mancozeb 8 parts, purple stem
Herba Lycopi 7 parts, 6 parts of carbonic acid, 5 parts of Pericarpium Zanthoxyli, solvent 28 parts.
Present invention also offers the preparation method of above-mentioned antibacterial, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Mirabilis jalapa, wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48 h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Radix Mirabilis extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Radix Mirabilis extract serosity of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Radix Mirabilis extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Eupatorium cannabinum L. (Eupatorium coelestinum L.) and Pericarpium Zanthoxyli are crushed to 4.5mm with plant pulverizer is minced, coarse powder is minced
Proportionally it is mixed and added into solvent with Mancozeb and carbonic acid and Radix Mirabilis extract that step (2) gained has been centrifuged is carried out
Dissolve, mix homogeneously, then grind 35 minutes with colloid mill, continue after adding antifreezing agent to grind 30 minutes on colloid mill,
To Fructus Litchi antibacterial colloid；
(4) stirring: Fructus Litchi antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi antibacterial
The 3/4 of colloid full dose, stirs under the rotating speed of 8000 revs/min 30 minutes, i.e. obtains Fructus Litchi disinfectant aqueous emulsion.
Below by following application experiment, the effect of Fructus Litchi antibacterial of the present invention is further described:
Experiment 1: select above-described embodiment 1 to 5 to carry out having a competition with Mancozeb, Eupatorium cannabinum L. (Eupatorium coelestinum L.), Radix Mirabilis extract respectively
Test.Disease collection on litchi in orchard being classified from Silin town, Bose City Tiandong County peasant household Litchi Garden, classification obtains
Fructus Litchi anthrax, Fructus Litchi powdery mildew, Fructus Litchi graywall, Fructus Litchi shot hole, Fructus Litchi algae pinta.Reference examples use Mancozeb,
Eupatorium cannabinum L. (Eupatorium coelestinum L.), Radix Mirabilis extract, result of the test is shown in Table 1
Table 1 Fructus Litchi of the present invention antibacterial and the efficiency test correction data of reference examples antibacterial single dose
| Fructus Litchi anthrax control rate (%) | Fructus Litchi graywall control rate (%) | Fructus Litchi controlling powdery mildew rate (%) | Fructus Litchi shot hole control rate (%) | Fructus Litchi algae pinta control rate (%) | |
| Embodiment 1 | 80.25 | 82.68 | 82.57 | 84.63 | 85.85 |
| Embodiment 2 | 82.3 | 85.78 | 82.34 | 84.27 | 84.31 |
| Embodiment 3 | 84.25 | 85.17 | 83.54 | 88.68 | 86.67 |
| Embodiment 4 | 86.54 | 88.58 | 85.28 | 87.46 | 88.19 |
| Embodiment 5 | 89.3 | 90.25 | 88.53 | 92.75 | 88.46 |
| Mancozeb | 60.22 | 60.17 | 58.44 | 54.85 | 58.62 |
| Eupatorium cannabinum L. (Eupatorium coelestinum L.) | 56.64 | 54 | 55.83 | 53.35 | 56.76 |
| Radix Mirabilis extract | 64.78 | 52.85 | 56.85 | 59.96 | 47.81 |
Higher preventive effect, average preventive effect is all had to reach five kinds for examination disease by Fructus Litchi antibacterial of the present invention seen from table 1 data
85.95%, and using the average preventive effect of Mancozeb is 58.46%, less than the average preventive effect of embodiment 27.49%；Eupatorium cannabinum L. (Eupatorium coelestinum L.)
Average preventive effect 55.32%, less than the average preventive effect of embodiment 30.63%；The average preventive effect of Radix Mirabilis extract is 56.45%, is less than
The average preventive effect of embodiment 29.49%.Seeing on the whole, the average preventive effect of reference examples is 56.74%, and the average preventive effect of the present embodiment is higher than
Reference examples 29.21%.Although reference examples also has preventive effect to reach 64.78%, but is only limitted to single disease, other disease is prevented
Effect is general, and fungicidal spectrum is limited.And the preventive effect of Fructus Litchi antibacterial of the present invention is not limited only to test five that a provided embodiment is corresponding
Plant disease, other litchi disease such as Fructus Litchi melasma, Fructus Litchi early blight and Fructus Litchi bleeding disease etc. are all had above experiment effect
Suitable preventive effect.
Experiment 2: select above-described embodiment 1 to 5 to carry out contrast test with Mancozeb, Eupatorium cannabinum L. (Eupatorium coelestinum L.), carbendazim respectively.From
Disease collection on litchi in orchard is classified by Silin town, Bose City Tiandong County peasant household Litchi Garden, has obtained Fructus Litchi anthrax
Disease, Fructus Litchi powdery mildew and Fructus Litchi shot hole, reference examples uses Mancozeb, Eupatorium cannabinum L. (Eupatorium coelestinum L.), Radix Mirabilis extract, respectively at head
The secondary later moon, three months and six months used are again to the Fructus Litchi anthrax collected, Fructus Litchi powdery mildew and Fructus Litchi
Shot hole carries out effect experiment, and contrast obtains the Drug resistance power that several drugs produces.Result of the test is shown in Table 2.
Table 2 Fructus Litchi of the present invention antibacterial and reference examples antibacterial single dose are for the resistant test of Fructus Litchi anthrax
By table 2 experimental data, the Drug resistance of Fructus Litchi anthrax is tested, by observing it is found that embodiment is executed first
Averagely declining 2.13% by six months afterwards preventive effects, reference examples Mancozeb medicament preventive effect after using six months first averagely drops
Low by 17.98%, compare embodiment and to exceed 15.65%；Reference examples Eupatorium cannabinum L. (Eupatorium coelestinum L.) preventive effect after using six months first averagely drops
Low by 16.18%, compare embodiment and to exceed 14.05%；Reference examples Radix Mirabilis extract preventive effect after using six months first
Averagely reduce 22.31%, compare embodiment and to exceed 20.18%.See that the preventive effect of three reference examples is used first on the whole
Averagely have dropped 18.83%. explanation after six months and use rear Fructus Litchi antibacterial of the present invention first to the not generation of Fructus Litchi anthrax
Significantly Drug resistance, and three reference examples all receive drug-fast impact for the preventive effect of Fructus Litchi anthrax and in various degree
Reduce.
Table 3 Fructus Litchi of the present invention antibacterial and reference examples antibacterial single dose are for the resistant test of Fructus Litchi powdery mildew
By table 3 experimental data, the Drug resistance of Fructus Litchi powdery mildew is tested, by observing it is found that embodiment is executed first
Averagely declining 1.74% by six months afterwards preventive effects, reference examples Mancozeb medicament preventive effect after using six months first averagely drops
Low by 15.59%, compare embodiment and to exceed 13.85%；Reference examples Eupatorium cannabinum L. (Eupatorium coelestinum L.) preventive effect after using six months first averagely drops
Low by 14.96%, compare embodiment and to exceed 13.22%；Reference examples Radix Mirabilis extract preventive effect after using six months first
Averagely reduce 21.53%, compare embodiment and to exceed 19.79%.See that the preventive effect of three reference examples is used first on the whole
17.36% is averagely have dropped after six months.Illustrate that using rear Fructus Litchi antibacterial of the present invention first does not produce Fructus Litchi powdery mildew
Significantly Drug resistance, and three reference examples all receive drug-fast impact for the preventive effect of Fructus Litchi powdery mildew and in various degree
Reduce.
Table 4 Fructus Litchi of the present invention antibacterial and reference examples antibacterial single dose are for the resistant test of Fructus Litchi shot hole
By table 4 experimental data, the Drug resistance of Fructus Litchi shot hole is tested, by observing it is found that embodiment is executed first
Averagely declining 1.87% by six months afterwards preventive effects, reference examples Mancozeb medicament preventive effect after using six months first averagely drops
Low by 14.53%, compare embodiment and to exceed 12.66%；Reference examples Eupatorium cannabinum L. (Eupatorium coelestinum L.) preventive effect after using six months first averagely drops
Low by 15.63%, compare embodiment and to exceed 13.58%；Reference examples Radix Mirabilis extract preventive effect after using six months first
Averagely reduce 27.54%, compare embodiment and to exceed 25.67%.See that the preventive effect of three reference examples is used first on the whole
18.87% is averagely have dropped after six months.Illustrate that using rear Fructus Litchi antibacterial of the present invention first does not produce Fructus Litchi shot hole
Significantly Drug resistance, and three reference examples all receive drug-fast impact for the preventive effect of Fructus Litchi shot hole and in various degree
Reduce.
Tables 2, table 3, the data of table 4 that experiment two is drawn are not limited only to Fructus Litchi anthrax, Fructus Litchi powdery mildew and Fructus Litchi skin ulcer
Scall, for other litchi disease, such as Fructus Litchi melasma, Fructus Litchi early blight and Fructus Litchi bleeding disease etc. all can effectively be alleviated
Drug-fast generation.
The invention is not limited in aforesaid detailed description of the invention.The present invention expands to any disclose in this manual
New feature or any new combination, and the arbitrary new method that discloses or the step of process or any new combination.
Claims (7)
1. a Fructus Litchi antibacterial, it is characterised in that be made up of the raw material of following parts by weight: Radix Mirabilis extract 12-22 part,
Mancozeb 5-12 part, Eupatorium cannabinum L. (Eupatorium coelestinum L.) 5-10 part, carbonic acid 3-9 part, Pericarpium Zanthoxyli 2-7 part, solvent 15-35 part.
A kind of Fructus Litchi antibacterial the most according to claim 1, it is characterised in that be made up of the raw material of following parts by weight: purple
Jasmine extract 15-20 part, Mancozeb 7-10 part, Eupatorium cannabinum L. (Eupatorium coelestinum L.) 6-8 part, carbonic acid 5-7 part, Pericarpium Zanthoxyli 4-6 part, solvent 24-31
Part.
A kind of Fructus Litchi antibacterial the most according to claim 1, it is characterised in that be made up of the raw material of following parts by weight: purple
Jasmine extract 18 parts, Mancozeb 8 parts, Eupatorium cannabinum L. (Eupatorium coelestinum L.) 7 parts, 6 parts of carbonic acid, 5 parts of Pericarpium Zanthoxyli, solvent 28 parts.
4. according to a kind of Fructus Litchi antibacterial described in claim 1 or 2 or 3, it is characterised in that: the solvent in described raw material is flower
One or more in oil generation, Oleum Brassicae campestris, soybean oil, Oleum sesami, Petiolus Trachycarpi oil, olive oil, Semen Maydis oil and mineral oil.
A kind of Fructus Litchi antibacterial the most according to claim 4, it is characterised in that: described solvent is Oleum sesami.
6. according to the preparation method of a kind of Fructus Litchi antibacterial described in claim 1 or 2 or 3, it is characterised in that comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Mirabilis jalapa, wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48h, after extracting solution filter 23 time, then is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Radix Mirabilis extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Radix Mirabilis extract serosity of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Radix Mirabilis extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Eupatorium cannabinum L. (Eupatorium coelestinum L.) and Pericarpium Zanthoxyli are crushed to 3-5mm with plant pulverizer is minced, coarse powder is minced
Proportionally it is mixed and added into solvent with Mancozeb and carbonic acid and Radix Mirabilis extract that step (2) gained has been centrifuged is carried out
Dissolve, mix homogeneously, then grind 20-40 minute with colloid mill, continue on colloid mill, grind 20-40 after adding antifreezing agent and divide
Clock, obtains Fructus Litchi antibacterial colloid；
(4) stirring: Fructus Litchi antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi antibacterial
The 3/4 of colloid full dose, stirs under the rotating speed of 2000-18000 rev/min 20-40 minute, i.e. obtains Fructus Litchi antibacterial water and milk
Agent.
The preparation method of a kind of Fructus Litchi antibacterial, it is characterised in that in step (4), described in stir
Mix method as follows: move in emulsifying kettle by Fructus Litchi antibacterial colloid, add distilled water to the 3/4 of Fructus Litchi antibacterial colloid full dose,
Stir under the rotating speed of 8000 revs/min 30 minutes, i.e. obtain Fructus Litchi disinfectant aqueous emulsion.
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