CN1788570A - Purple stem boneset extract for prevention and treatment of plant disease - Google Patents
Purple stem boneset extract for prevention and treatment of plant disease Download PDFInfo
- Publication number
- CN1788570A CN1788570A CN 200510048759 CN200510048759A CN1788570A CN 1788570 A CN1788570 A CN 1788570A CN 200510048759 CN200510048759 CN 200510048759 CN 200510048759 A CN200510048759 A CN 200510048759A CN 1788570 A CN1788570 A CN 1788570A
- Authority
- CN
- China
- Prior art keywords
- extract
- eupatorium adenophorum
- plant disease
- control
- stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The present invention relates to plant disease prevention and control with plant extract, and is especially purplestem eupatorium extract for plant disease prevention and control. The n-hexane and petroleum ether extract of purplestem eupatorium has high inhibiting activity on the pathogenic bacteria causing foot rot of broad bean, early blight of tomato, rice blast and pear scab. The purplestem eupatorium extract may be used to prepare germicide with other natural plant extracts, and the germicide has excellent prevention and control effect on plant diseases. The present invention utilizes purplestem eupatorium extract in preventing and controlling plant diseases for the first time.
Description
Technical field
The present invention relates to purple stem boneset extract and be used for control of plant disease, belong to the extraction and the application of botanical pesticide.
Background technology
Eupatorium adenophorum (Eupatouium adenophorum) English name Crofton weed or Pamakani have another name called and destroy grass, fragrant eupatorium herb, liberation grass etc., are a kind of herbaceos perennials of composite family Eupatorium.Eupatorium adenophorum has that the ecologic adaptation scope is wide, fast growth, fertility is strong, gregariousness is high, aggressiveness and strong stress resistance, consume characteristic such as soil fertility in a large number, can breed in a large number at short notice, form the excellent plant community of list of intensive Cheng Cong, cause many plant growings slow, some species even endangered, and grain, high quality forage etc. drops in production over a large area, and is a kind of global malignant weed.
About the control of Eupatorium adenophorum, that usually adopts has physical mechanical method, manually anti-division, chemical control method, a measure of biotic control etc.Because Eupatorium adenophorum is a perennial weed, fast growth has a very wide distribution, and only depends on passive preventing and kill off, and it spreads and endangers to be difficult to control.Fundamentally control this malignant weed of Eupatorium adenophorum, should set about from its positive purposes, how research will prevent and kill off with utilization and combine, and change evil and be profit, turns waste into wealth.
Research about the Eupatorium adenophorum purposes, the doctor Wang Yiding of Sichuan University studies Eupatorium adenophorum being used for anti-eliminating aphis, think that the plain A of the Eupatorium adenophorum of extracting has tangible virulence activity to aphid from Eupatorium adenophorum, and in its thesis for the doctorate, introduced result of study.Simultaneously, Wang Yiding also mentions the expensive people's of grade of Sichuan University's bio-engineering research Liu of institute generation research and thinks in its thesis for the doctorate, purple stem boneset extract yet is not used for purple stem boneset extract the precedent of control of plant disease both at home and abroad at present to fungi unrestraint effect.
Summary of the invention
The present invention has overcome the deficiency to purple stem boneset extract purposes understanding and practice, provides the control of plant disease of purple stem boneset extract to use and the preparation method.
The application of purple stem boneset extract in control of plant disease.Described plant disease is crops root rot, crops early blight, crops late blight, crop powdery mildew, crops downy mildew, crops eqpidemic disease, crops black spot, crops brown spot, rice blast, pear scab.
Described purple stem boneset extract is the extract of Eupatorium adenophorum root or stem or leaf or flower or seed.
A kind of preparation method of purple stem boneset extract the steps include:
1. resolve into root, stem, leaf, flower, seed after the earth Ex-all with the Eupatorium adenophorum root, in 40～60 ℃ baking oven dry 5～72 hours respectively, with dried Eupatorium adenophorum tissue with the pulverizing of plant cracker;
2. the weight that the Eupatorium adenophorum tissue powder minced and solvent volume ratio are 1: 2～25 solvent, soak and extract 1～5 filter cleaner, and extraction time is 5～72 hours, and extracting temperature is 20～95 ℃;
3. the concentrating under reduced pressure under 30～90 ℃ temperature of the extract after will filtering is produced purple stem boneset extract medicinal extract.
Described solvent is one or more of n-hexane, benzinum, carrene, chloroform, ethyl acetate, toluene, dimethylbenzene, acetone, methyl alcohol, ethanol, water.
The purple stem boneset extract medicinal extract that obtains by above preparation method can be directly used in the control of plant disease or with being processed into preparation after the purple stem boneset extract medicinal extract separation purification, be used for the control of plant disease.
The present invention has started the precedent that purple stem boneset extract is used for control of plant disease, has remedied the deficiency of Eupatorium adenophorum being utilized understanding, has negated the viewpoint of thinking that in the past purple stem boneset extract is invalid to plant disease.Think that in the past purple stem boneset extract is invalid to plant disease, be because extracting method and technology defectiveness, particularly solvent ratios in the extracting method and the selection of other technical factors are improper, the present invention has selected appropriate extraction process and method, makes purple stem boneset extract be used for control of plant disease and becomes a reality.Purple stem boneset extract is used for control of plant disease has control efficiency good, the low characteristics of control cost.Effectively Eupatorium adenophorum is prevented and kill off with utilization and combines, change evil for sharp, turn waste into wealth, have good environment and ecological benefits.
Embodiment
Embodiment one
The Eupatorium adenophorum stem 1kg that root, leaf, flower are removed in taking-up in 40 ℃ baking oven dry 5 hours, pulverizing the back with the plant cracker, to add mass volume ratio be that 1: 2 n-hexane soaks and extracts 3 filter cleaners, and each extraction time is 5 hours, and the extraction temperature is 20 ℃.Extract after filtering is directly obtained the n-hexane extract medicinal extract of Eupatorium adenophorum stem after concentrating under 30 ℃ the temperature.
The n-hexane extract medicinal extract of the Eupatorium adenophorum stem that embodiment one makes is to the control efficiency of plant disease
| Test medicine and using dosage | Medication after 10 days to the control efficiency (%) of disease | |
| Early blight of tomato | Rice blast | |
| The n-hexane extract medicinal extract 80 gram/mus 10 polyoxin wettables of the n-hexane extract medicinal extract 60 gram/mu Eupatorium adenophorum stems of the n-hexane extract medicinal extract 40 gram/mu Eupatorium adenophorum stems of Eupatorium adenophorum stem divide agent 50 gram/mus | 62.4 73.2 78.5 75.3 | 65.3 75.6 77.1 71.6 |
Embodiment two
The Eupatorium adenophorum stem 1kg that root, leaf, flower are removed in taking-up in 55 ℃ baking oven dry 24 hours, pulverizing the back with the plant cracker, to add mass volume ratio be that 1: 4 n-hexane soaks and extracts 3 filter cleaners, and each extraction time is 24 hours, and the extraction temperature is 40 ℃.Extract after filtering is directly obtained the n-hexane extract medicinal extract of Eupatorium adenophorum stem after concentrating under 45 ℃ the temperature.
The n-hexane extract medicinal extract of the Eupatorium adenophorum stem that embodiment two makes is to the control efficiency of plant disease
| Test medicine and using dosage | Medication after 10 days to the control efficiency (%) of disease | |
| Early blight of tomato | Rice blast | |
| The n-hexane extract medicinal extract 80 gram/mus 10 polyoxin wettables of the n-hexane extract medicinal extract 60 gram/mu Eupatorium adenophorum stems of the n-hexane extract medicinal extract 40 gram/mu Eupatorium adenophorum stems of Eupatorium adenophorum stem divide agent 50 gram/mus | 68.6 75.8 78.5 77.1 | 69.5 77.2 80.9 74.2 |
Embodiment three
The Eupatorium adenophorum stem 1kg that root, leaf, flower are removed in taking-up in 60 ℃ baking oven dry 72 hours, pulverizing the back with the plant cracker, to add mass volume ratio be that 1: 25 n-hexane soaks and extracts 3 filter cleaners, each extraction time is 72 hours, and extracting temperature is 95 ℃.Extract after filtering is directly obtained the n-hexane extract medicinal extract of Eupatorium adenophorum stem after concentrating under 90 ℃ the temperature.
The n-hexane extract medicinal extract of the Eupatorium adenophorum stem that embodiment three makes is to the control efficiency of plant disease
| Test medicine and using dosage | Medication after 10 days to the control efficiency (%) of disease | |
| Early blight of tomato | Rice blast | |
| The n-hexane extract medicinal extract 80 gram/mus 10 polyoxin wettables of the n-hexane extract medicinal extract 60 gram/mu Eupatorium adenophorum stems of the n-hexane extract medicinal extract 40 gram/mu Eupatorium adenophorum stems of Eupatorium adenophorum stem divide agent 50 gram/mus | 59.7 62.1 65.4 78.5 | 61.4 63.9 67.8 73.1 |
Embodiment four
The Eupatorium adenophorum stem 1kg that root, leaf, flower are removed in taking-up in 40 ℃ baking oven dry 5 hours, pulverizing the back with the plant cracker, to add mass volume ratio be that 1: 2 benzinum soaks and extracts 3 filter cleaners, and each extraction time is 5 hours, and the extraction temperature is 20 ℃.Extract after filtering is directly obtained the ligroin extraction medicinal extract of Eupatorium adenophorum stem after concentrating under 30 ℃ the temperature.
The ligroin extraction medicinal extract of the Eupatorium adenophorum stem that embodiment four makes is to the control efficiency of plant disease
| Test medicine and using dosage | Medication after 10 days to the control efficiency (%) of disease | |
| Early blight of tomato | Rice blast | |
| The ligroin extraction medicinal extract 80 gram/mus 10 polyoxin wettables of the ligroin extraction medicinal extract 60 gram/mu Eupatorium adenophorum stems of the ligroin extraction medicinal extract 40 gram/mu Eupatorium adenophorum stems of Eupatorium adenophorum stem divide agent 50 gram/mus | 64.5 72.7 75.2 74.7 | 62.7 73.6 74.8 72.8 |
Embodiment five
The Eupatorium adenophorum stem 1kg that root, leaf, flower are removed in taking-up in 55 ℃ baking oven dry 24 hours, pulverizing the back with the plant cracker, to add mass volume ratio be that 1: 4 benzinum soaks and extracts 3 filter cleaners, and each extraction time is 24 hours, and the extraction temperature is 40 ℃.Extract after filtering is directly obtained the ligroin extraction medicinal extract of Eupatorium adenophorum stem after concentrating under 45 ℃ the temperature.
The ligroin extraction medicinal extract of the Eupatorium adenophorum stem that embodiment five makes is to the control efficiency of plant disease
| Test medicine and using dosage | Medication after 10 days to the control efficiency (%) of disease | |
| Early blight of tomato | Rice blast | |
| The ligroin extraction medicinal extract 80 gram/mus 10 polyoxin wettables of the ligroin extraction medicinal extract 60 gram/mu Eupatorium adenophorum stems of the ligroin extraction medicinal extract 40 gram/mu Eupatorium adenophorum stems of Eupatorium adenophorum stem divide agent 50 gram/mus | 66.8 74.3 76.9 76.9 | 65.4 76.1 78.2 73.6 |
Embodiment six
The Eupatorium adenophorum stem 1kg that root, leaf, flower are removed in taking-up in 60 ℃ baking oven dry 72 hours, pulverizing the back with the plant cracker, to add mass volume ratio be that 1: 25 benzinum soaks and extracts 3 filter cleaners, each extraction time is 72 hours, and extracting temperature is 95 ℃.Extract after filtering is directly obtained the ligroin extraction medicinal extract of Eupatorium adenophorum stem after concentrating under 90 ℃ the temperature.
The ligroin extraction medicinal extract of the Eupatorium adenophorum stem that embodiment six makes is to the control efficiency of plant disease
| Test medicine and using dosage | Medication after 10 days to the control efficiency (%) of disease | |
| Early blight of tomato | Rice blast | |
| The ligroin extraction medicinal extract 80 gram/mus 10 polyoxin wettables of the ligroin extraction medicinal extract 60 gram/mu Eupatorium adenophorum stems of the ligroin extraction medicinal extract 40 gram/mu Eupatorium adenophorum stems of Eupatorium adenophorum stem divide agent 50 gram/mus | 62.4 64.6 69.3 79.3 | 63.9 65.3 70.4 76.9 |
Purple stem boneset extract is to the indoor bioassay of plant disease:
Measure 1
According to preparation method with malicious medium, the PDA that makes content and be the n-hexane extract medicinal extract of 0.1% Eupatorium adenophorum stem is with malicious medium, the pathogen (Fusarium Solani) of inoculation broad bean root rot, the n-hexane extract medicinal extract of Eupatorium adenophorum stem is 100% to the inhibiting rate of broad bean root rot pathogen (Fusarium Solani).
Measure 2
According to preparation method with malicious medium, the PDA that makes content and be the n-hexane extract medicinal extract of 0.1% Eupatorium adenophorum stem is with malicious medium, the pathogen of inoculating tomato early blight (Alternaria Solani), the n-hexane extract medicinal extract of Eupatorium adenophorum stem is 100% to the inhibiting rate of early blight of tomato pathogen (Alternaria Solani).
Measure 3
According to preparation method with malicious medium, the PDA that makes content and be the n-hexane extract medicinal extract of 0.1% Eupatorium adenophorum stem is with malicious medium, the pathogen (Venturia pyrina) of inoculation pear scab, the n-hexane extract medicinal extract of Eupatorium adenophorum stem is 100% to the inhibiting rate of the pathogen (Venturia pyrina) of pear scab.
Measure 4
According to preparation method with malicious medium, the PDA that makes content and be the n-hexane extract medicinal extract of 0.1% Eupatorium adenophorum stem is with malicious medium, the pathogen (Pyricularia oryzae) of inoculation rice blast, the n-hexane extract medicinal extract of Eupatorium adenophorum stem is 100% to the inhibiting rate of the pathogen (Pyricularia oryzae) of rice blast.
Measure 5
According to preparation method with malicious medium, the PDA that makes content and be the ligroin extraction medicinal extract of 0.1% Eupatorium adenophorum stem is with malicious medium, the pathogen (Fusarium solani) of inoculation broad bean root rot, the ligroin extraction medicinal extract of Eupatorium adenophorum stem is 100% to the inhibiting rate of broad bean root rot pathogen (Fusarium solani).
Measure 6
According to preparation method with malicious medium, the PDA that makes content and be the ligroin extraction medicinal extract of 0.1% Eupatorium adenophorum stem is with malicious medium, the pathogen of inoculating tomato early blight (Alternaria solani), the ligroin extraction medicinal extract of Eupatorium adenophorum stem is 100% to the inhibiting rate of early blight of tomato pathogen (Alternaria solani).
Measure 7
According to preparation method with malicious medium, the PDA that makes content and be the ligroin extraction medicinal extract of 0.1% Eupatorium adenophorum stem is with malicious medium, the pathogen (Venturia pyrina) of inoculation pear scab, the ligroin extraction medicinal extract of Eupatorium adenophorum stem is 100% to the inhibiting rate of the pathogen (Venturia pyrina) of pear scab.
Measure 8
According to preparation method with malicious medium, the PDA that makes content and be the ligroin extraction medicinal extract of 0.1% Eupatorium adenophorum stem is with malicious medium, the pathogen (Pyricularia oryzae) of inoculation rice blast, the ligroin extraction medicinal extract of Eupatorium adenophorum stem is 100% to the inhibiting rate of the pathogen (Pyricularia oryzae) of rice blast.
Claims (5)
1. purple stem boneset extract is used for control of plant disease, it is characterized in that: the application of purple stem boneset extract in control of plant disease.
2. purple stem boneset extract according to claim 1 is used for control of plant disease, it is characterized in that: described plant disease is crops root rot, crops early blight, crops late blight, crop powdery mildew, crops downy mildew, crops eqpidemic disease, crops black spot, crops brown spot, rice blast, pear scab.
3. purple stem boneset extract according to claim 1 is used for control of plant disease, it is characterized in that: described purple stem boneset extract is the extract of Eupatorium adenophorum root or stem or leaf or flower or seed.
4. the preparation method of a purple stem boneset extract the steps include:
(1) resolve into root, stem, leaf, flower, seed after the earth Ex-all with the Eupatorium adenophorum root, in 40～60 ℃ baking oven dry 5～72 hours respectively, with dried Eupatorium adenophorum tissue with the pulverizing of plant cracker;
(2) the Eupatorium adenophorum tissue powder is minced weight and solvent volume ratio is 1: 2～25 solvent, soaks and extracts 1～5 filter cleaner, and extraction time is 5～72 hours, and extracting temperature is 20～95 ℃;
(3) the extract concentrating under reduced pressure under 30～90 ℃ temperature after will filtering is produced purple stem boneset extract medicinal extract.
5. purple stem boneset extract according to claim 4 is used for control of plant disease, it is characterized in that: described solvent is one or more of n-hexane, benzinum, carrene, chloroform, ethyl acetate, toluene, dimethylbenzene, acetone, methyl alcohol, ethanol, water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100487598A CN100340171C (en) | 2005-12-27 | 2005-12-27 | Purple stem boneset extract for prevention and treatment of plant disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100487598A CN100340171C (en) | 2005-12-27 | 2005-12-27 | Purple stem boneset extract for prevention and treatment of plant disease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1788570A true CN1788570A (en) | 2006-06-21 |
| CN100340171C CN100340171C (en) | 2007-10-03 |
Family
ID=36786848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100487598A Expired - Fee Related CN100340171C (en) | 2005-12-27 | 2005-12-27 | Purple stem boneset extract for prevention and treatment of plant disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100340171C (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100463604C (en) * | 2006-08-09 | 2009-02-25 | 云南师范大学 | Method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice |
| CN101525508A (en) * | 2008-03-06 | 2009-09-09 | 中国农业科学院植物保护研究所 | Application of Eupatorium adenophorum extract as anti-fouling paint antifoulant |
| CN101933533A (en) * | 2010-07-20 | 2011-01-05 | 华南农业大学 | Plant source preservative and preparation method and application thereof |
| CN101947327A (en) * | 2010-08-30 | 2011-01-19 | 江门市和馨香精技术有限公司 | Deodorant |
| CN101664046B (en) * | 2009-09-23 | 2013-01-30 | 云南农业大学 | Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof |
| CN103461326A (en) * | 2013-09-10 | 2013-12-25 | 苏州市丰澄农业专业合作社 | Chromolaena odorata plant based nano slow-release suspending agent |
| CN104402638A (en) * | 2014-12-22 | 2015-03-11 | 攀枝花市西宇生物科技有限公司 | Method for preparing special leaf fertilizer for mangoes from ageratina adenophora |
| CN104872198A (en) * | 2015-05-11 | 2015-09-02 | 河北工程大学 | High-efficiency compound pesticide composition containing plant extract, and preparation method of composition |
| CN105409920A (en) * | 2015-11-25 | 2016-03-23 | 云南农业大学 | Repelling method for nasonia nitripennis |
| CN106234442A (en) * | 2016-08-10 | 2016-12-21 | 吴远宁 | A kind of Fructus Litchi antibacterial and preparation method thereof |
| CN106900754A (en) * | 2017-01-22 | 2017-06-30 | 嵊州市派特普科技开发有限公司 | Lawn-care galenical and preparation method thereof |
| CN107711906A (en) * | 2017-10-24 | 2018-02-23 | 柳州华晟科技有限公司 | A kind of botanical fungicide for preventing and treating rice blast |
| CN107821450A (en) * | 2017-11-15 | 2018-03-23 | 湖南农业大学 | A kind of botanical fungicide composition containing purple stem boneset extract and its preparation method and application |
| CN108402096A (en) * | 2018-01-30 | 2018-08-17 | 广西壮歌农业科技桑博园有限责任公司 | A kind of mulberry sapling graft union agent |
| CN108496998A (en) * | 2018-01-30 | 2018-09-07 | 广西壮歌农业科技桑博园有限责任公司 | A kind of consolidant improving mulberry sapling graft survival rate |
| CN109662105A (en) * | 2018-11-20 | 2019-04-23 | 山西农业大学 | A method of extracting Fungicidal active substance from Eupatorium adenophorum plant leaf |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1287786A (en) * | 2000-10-08 | 2001-03-21 | 四川九章生物化工科技发展有限公司 | Method for preparing biological broad spectrum insecticide |
| CN1389437A (en) * | 2002-07-06 | 2003-01-08 | 欧阳华 | Production process of biological organic fertilizer with purplestem eupatorium |
| CN1218643C (en) * | 2002-12-11 | 2005-09-14 | 四川大学 | Eupatorine pesticide and its prepn |
| CN100426963C (en) * | 2005-01-28 | 2008-10-22 | 南京农业大学 | Use of metabolite of eupatorium in biologically herbicidal |
-
2005
- 2005-12-27 CN CNB2005100487598A patent/CN100340171C/en not_active Expired - Fee Related
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100463604C (en) * | 2006-08-09 | 2009-02-25 | 云南师范大学 | Method for inhibiting plant pathogenic bacteria by using Eupatorium adenophorum juice |
| CN101525508A (en) * | 2008-03-06 | 2009-09-09 | 中国农业科学院植物保护研究所 | Application of Eupatorium adenophorum extract as anti-fouling paint antifoulant |
| CN101525508B (en) * | 2008-03-06 | 2013-07-24 | 中国农业科学院植物保护研究所 | Application of Eupatorium adenophorum extract as anti-fouling paint antifoulant |
| CN101664046B (en) * | 2009-09-23 | 2013-01-30 | 云南农业大学 | Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof |
| CN101933533A (en) * | 2010-07-20 | 2011-01-05 | 华南农业大学 | Plant source preservative and preparation method and application thereof |
| CN101933533B (en) * | 2010-07-20 | 2013-06-05 | 华南农业大学 | Plant source preservative and preparation method and application thereof |
| CN101947327A (en) * | 2010-08-30 | 2011-01-19 | 江门市和馨香精技术有限公司 | Deodorant |
| CN101947327B (en) * | 2010-08-30 | 2013-11-06 | 江门市和馨香精技术有限公司 | Deodorant |
| CN103461326A (en) * | 2013-09-10 | 2013-12-25 | 苏州市丰澄农业专业合作社 | Chromolaena odorata plant based nano slow-release suspending agent |
| CN104402638A (en) * | 2014-12-22 | 2015-03-11 | 攀枝花市西宇生物科技有限公司 | Method for preparing special leaf fertilizer for mangoes from ageratina adenophora |
| CN104872198A (en) * | 2015-05-11 | 2015-09-02 | 河北工程大学 | High-efficiency compound pesticide composition containing plant extract, and preparation method of composition |
| CN105409920A (en) * | 2015-11-25 | 2016-03-23 | 云南农业大学 | Repelling method for nasonia nitripennis |
| CN105409920B (en) * | 2015-11-25 | 2017-11-21 | 云南农业大学 | A kind of repellent method of Pupal parasite |
| CN106234442A (en) * | 2016-08-10 | 2016-12-21 | 吴远宁 | A kind of Fructus Litchi antibacterial and preparation method thereof |
| CN106900754A (en) * | 2017-01-22 | 2017-06-30 | 嵊州市派特普科技开发有限公司 | Lawn-care galenical and preparation method thereof |
| CN107711906A (en) * | 2017-10-24 | 2018-02-23 | 柳州华晟科技有限公司 | A kind of botanical fungicide for preventing and treating rice blast |
| CN107821450A (en) * | 2017-11-15 | 2018-03-23 | 湖南农业大学 | A kind of botanical fungicide composition containing purple stem boneset extract and its preparation method and application |
| CN108402096A (en) * | 2018-01-30 | 2018-08-17 | 广西壮歌农业科技桑博园有限责任公司 | A kind of mulberry sapling graft union agent |
| CN108496998A (en) * | 2018-01-30 | 2018-09-07 | 广西壮歌农业科技桑博园有限责任公司 | A kind of consolidant improving mulberry sapling graft survival rate |
| CN109662105A (en) * | 2018-11-20 | 2019-04-23 | 山西农业大学 | A method of extracting Fungicidal active substance from Eupatorium adenophorum plant leaf |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100340171C (en) | 2007-10-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100340171C (en) | Purple stem boneset extract for prevention and treatment of plant disease | |
| CN102835245B (en) | Bionic culture method for cordyceps sobolifera | |
| CN101054565A (en) | Biological control strain capable of preventing and curing root knot nematode disease for greenhouse vegetable | |
| CN106801014A (en) | A kind of endogenetic fungus and its application for improving red sage root yield and its active constituent content | |
| CN109266559B (en) | A kind of application of Trichoderma harzianum LTR-2 | |
| Chen et al. | Botrytis cinerea causing gray mold of Polygonatum sibiricum (Huang Jing) in China | |
| CN113956983A (en) | Atractylodes macrocephala endophytic fungus, fermentation product and application thereof | |
| CN102604857A (en) | Biological control pseudomonas monteilii strain against tobacco mosaic virus (TMV) | |
| CN115044487A (en) | Marine fungus strain with tobacco mosaic virus prevention and control effect | |
| CN110178857B (en) | Application of seabuckthorn endophytic fungus strain SJ1 fermentation extract | |
| CN114540205A (en) | Armillaria mellea strain and application thereof | |
| Senapati et al. | Evaluation of antimicrobial activity of Azadirachta indica bark extract | |
| CN101790996A (en) | Antiviral biopesticide sourcing from Eupatorium adenophorum spreng and preparation method thereof | |
| CN107475123B (en) | Anoectochilus roxburghii endophytic fungus and application thereof | |
| CN107494603B (en) | Natural plant growth regulator and preparation method and application thereof | |
| CN111418595A (en) | Application of C21 steroid compounds 1-8 in paniculate swallowwort root in preparing anti-TSWV active product | |
| Rizk et al. | Biological Control of Fusarium Oxysporum and Verticillium dahliaeBy Trichoderma harzianum and Gliocladium virens of Two Mint Species | |
| CN113684137B (en) | Radix scrophulariae endophytic fungus and application thereof | |
| CN114946895B (en) | Application of amomum tsao-ko essential oil | |
| CN115152798B (en) | Application and composition of Rapex baileyi PR2 extract | |
| CN111406750A (en) | Use of F01 polysaccharide for antivirus | |
| CN115399345B (en) | Composition containing paris polyphylla extract and preparation method and application thereof | |
| CN110477037B (en) | Plant source pesticide composition and preparation method and application thereof | |
| CN114921358B (en) | Geobacillus strain and application thereof | |
| CN113502227B (en) | Fusarium vine, microbial inoculum and herbicide containing same and application of Fusarium vine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20071003 Termination date: 20101227 |