CN111903688A - Application of 6-pentyl-2H-pyran-2-one in preparation of anti-Sclerotinia delphinii pathogenic bacteria agent - Google Patents
Application of 6-pentyl-2H-pyran-2-one in preparation of anti-Sclerotinia delphinii pathogenic bacteria agent Download PDFInfo
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- CN111903688A CN111903688A CN202010896607.8A CN202010896607A CN111903688A CN 111903688 A CN111903688 A CN 111903688A CN 202010896607 A CN202010896607 A CN 202010896607A CN 111903688 A CN111903688 A CN 111903688A
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- pyran
- southern blight
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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Abstract
The invention discloses an application of a 6-pentyl-2H-pyran-2-one compound in preparation of an anti-Sclerotinia delphinii pathogenic bacteria agent. The 6-amyl-2H-pyran-2-ketone compound is called 6-PP for short, and the CAS number of the compound is as follows: CAS No.27593-23-3, having the formula:
the invention has the technical effects that: the invention discovers that the 6-amyl-2H-pyran-2-one compound has a remarkable inhibition effect on the growth of the southern blight of the dendrobium officinale, and has a good prevention and treatment effect on the southern blight of the dendrobium officinale. The 6-amyl-2H-pyran-2-one compound is a natural product with high efficiency and no toxicity, namely, the invention provides a natural pesticide with high efficiency and no toxicity for preventing and treating southern blight.
Description
Field of the invention
The invention relates to the technical field of natural medicines, in particular to application of a 6-pentyl-2H-pyran-2-one compound in preparation of an anti-Sclerotinia delphinii pathogenic bacteria agent.
Background
The dendrobium officinale is a traditional rare medicinal material and has the effects of nourishing yin, clearing heat, benefiting stomach, promoting fluid production and the likeAnd (5) effect. With the increase of market demand and the transformation and upgrading of industries, the wild cultivation of the dendrobium officinale can be rapidly developed. However, in the case of high humidity in the plum rain season, the bacteria are caused by Sclerotinia delphiniiSclerotium delphiniiThe caused southern blight becomes a destructive disease of the dendrobium officinale, and usually causes great economic loss in a main production area of the dendrobium officinale, but no effective prevention and control means exists so far.
The 6-amyl-2H-pyran-2-one compound is abbreviated as '6-PP', and the CAS number of the compound is as follows: CAS No.27593-23-3, a known compound, is commonly used as a food additive or flavor (flavorant) and also as a pharmaceutical intermediate. In the aspect of agricultural utilization, most researches on '6-PP' mainly focus on promoting plant growth, a few research reports on resisting plant diseases exist, and researches prove that the compound has inhibitory activity on various pathogenic bacteria, including fusarium oxysporum, sclerotinia sclerotiorum, aspergillus niger, rhizoctonia solani, alternaria solani, botrytis cinerea and the like. The structural formula is as follows:
trichoderma fungi produced by the inventors of the present invention (Trichoderma) Trichoderma fungi (Trichoderma) found in biological control of plant diseasesTrichoderma) Can produce a plurality of secondary metabolites, some metabolites have the function of antagonizing pathogenic microorganisms, and 6-amyl-2H-pyran-2-one ('6-PP') has better bacteriostatic effect. "6-PP" was originally found in Trichoderma viride culture and was subsequently obtained in Trichoderma harzianum, Trichoderma koningii and Trichoderma asperellum. Most researches on 6-PP mainly focus on promoting plant growth, and few research reports on resisting plant diseases exist, and the researches prove that the compound has inhibitory activity on various pathogenic bacteria, including fusarium oxysporum, sclerotinia sclerotiorum, aspergillus niger, rhizoctonia solani, alternaria solani, botrytis cinerea and the like.
Disclosure of Invention
In view of the above, the present invention aims to provide the use of a 6-pentyl-2H-pyran-2-one compound in the preparation of an anti-sclerotium delphinii pathogenic bacterial agent.
Experiments show that the '6-PP' has an obvious bacteriostatic action on sclerotium delphinii which is a pathogenic bacterium of southern blight, so that the 6-pentyl-2H-pyran-2-one compound can be applied to preparation of an anti-pathogenic bacterium agent of southern blight.
The structural formula of the 6-pentyl-2H-pyran-2-one compound is shown as the following formula:
the pathogenic bacteria of the dendrobium candidum southern blight is sclerotium delphiniiS. delphinii。
Preferably, the dendrobium candidum southern blight pathogenic bacterium resistant agent also comprises an agriculturally and pharmaceutically acceptable auxiliary agent.
Preferably, the anti-sclerotium rolfsii pathogenic bacteria agent also comprises other anti-sclerotium rolfsii medicines.
Preferably, the formulation of the sclerotium delphinium agent for resisting southern blight pathogenic bacteria is wettable powder, water dispersible granule, suspending agent or missible oil.
Preferably, the 6-pentyl-2H-pyran-2-one compound is 1 to 99% by weight of the anti-southern blight pathogenic agent.
More preferably, the 6-pentyl-2H-pyran-2-one compound accounts for 10 to 90 percent of the weight of the southern blight pathogenic bacteria resistant agent.
The invention has the technical effects that: the invention discovers that the 6-amyl-2H-pyran-2-one compound has a remarkable inhibition effect on the growth of the southern blight of the dendrobium officinale, and has a good prevention and treatment effect on the southern blight of the dendrobium officinale. The 6-amyl-2H-pyran-2-one compound is a natural product with high efficiency and no toxicity, namely, the invention provides a natural pesticide with high efficiency and no toxicity for preventing and treating southern blight.
Drawings
FIG. 1: the result of the qualitative test of 6-amyl-2H-pyran-2-ketone on the micronucleus delphinii;
FIG. 2: 6-amyl-2H-pyran-2-ketone and micronucleus delphinii quantitative test results;
FIG. 3: effect of different concentrations of 6-pentyl-2H-pyran-2-one on the growth of sclerotium delphinii.
Detailed Description
The invention discloses application of a 6-pentyl-2H-pyran-2-one compound in preparation of an anti-southern blight agent, and a person skilled in the art can use the content for reference and appropriately improve process parameters to realize the application. It is expressly intended that all such similar substitutes and modifications which would be obvious to those skilled in the art are deemed to be included within the invention.
Example 1: the 6-amyl-2H-pyran-2-ketone has an inhibiting effect on sclerotium delphinii which is a pathogen of the dendrobium candidum southern blight:
and (3) qualitative test: the inhibition effect of DCR2 metabolite '6-PP' on the growth of sclerotium delphinii hypha is measured by a paper sheet method. Respectively placing 5 mm sterile filter paper containing 6-PP and 5 mm Sclerotinia deltoides fungus cake on two points of PDA flat plate 30 mm away from center on the same straight line, culturing at 25 deg.C for 5 d, observing growth condition of Sclerotinia deltoides fungus colony, and repeating each treatment for 3 times.
Quantitative test: the inhibition effect of 6-PP on the growth of the sclerotium delphinii hyphae is measured by a colony diameter method. The final concentrations of "6-PP" in the prepared medium were 300, 200, 100 and 50. mu.g.mL, respectively-1. Activating Sclerotinia deltoides, extracting cake with diameter of 5 mm from the edge of cultured Sclerotinia deltoides colony, inoculating to plates with different concentrations, repeating each treatment for 3 times, culturing at 25 deg.C for 5 days, and measuring the radius R corresponding to the colony growth of the treated group with vernier caliper1And a reference radius R0And calculating an inhibition rate:
inhibition ratio (%) { 1- (R)0-R1）/R0｝100%。
Disease resistance results for "6-PP": through a paper sheet method, the '6-PP' is found to have obvious bacteriostatic effect on the sclerotinia delphinii (figure 1), a control group is a clean sterile filter paper sheet, the sclerotinia delphinii grows over the whole flat plate, the paper sheet is covered,while the group treated with the sterile filter paper containing the "6-PP" was significantly inhibited from Sclerotinia delphinii. Further investigation revealed that the "6-PP" concentration was 50, 100, 200. mu.g/mL, the more strongly the "6-PP" concentration inhibited Sclerotinia delphinii (FIG. 2)-1The inhibition rates of the strains on the Sclerotinia delphinii are respectively 60.3%, 67.5% and 83.0% (FIG. 3), and when the concentration of the "6-PP" reaches 300. mu.g/mL-1When the growth of the sclerotium delphinii mycelia is completely inhibited.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
- The application of the 6-pentyl-2H-pyran-2-one compound in preparing the agent for resisting the pathogenic bacteria of the dendrobium candidum southern blight is disclosed, wherein the structural formula of the 6-pentyl-2H-pyran-2-one compound is shown as the following formula:。
- 2. the use as claimed in claim 1, wherein the pathogenic bacterium of southern blight of Dendrobium officinale is Sclerotinia deltoideaS. delphinii。
- 3. The use of claim 1, wherein the anti-southern blight pathogenic agent of Dendrobium officinale further comprises an agriculturally and pharmaceutically acceptable adjuvant.
- 4. The use of claim 1, wherein the anti-southern blight pathogenic agent of Dendrobium officinale comprises other anti-southern blight drugs.
- 5. The use according to claim 1, 2, 3 or 4, wherein the anti-southern blight pathogenic agent of Dendrobium officinale is in the form of wettable powder, water dispersible granules, a suspending agent or emulsifiable concentrate.
- 6. The use according to claim 1, 2, 3 or 4, wherein the 6-pentyl-2H-pyran-2-one is present in an amount of 1 to 99% by weight of the agent against southern blight of Dendrobium officinale.
- 7. The use of claim 6, wherein the 6-pentyl-2H-pyran-2-one is present in an amount of 10 to 90% by weight of the agent against southern blight of Dendrobium officinale.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114258924A (en) * | 2021-12-30 | 2022-04-01 | 新疆农业科学院微生物应用研究所(中国新疆—亚美尼亚生物工程研究开发中心) | Application of trichoderma hedgehog in preparation of medicine for preventing and treating pear black spot |
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| US6060507A (en) * | 1994-02-07 | 2000-05-09 | Horticulture And Food Research Institute Of New Zealand Limited | Use of massoialactone for inhibition of fungal growth |
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- 2020-08-31 CN CN202010896607.8A patent/CN111903688A/en active Pending
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| US6060507A (en) * | 1994-02-07 | 2000-05-09 | Horticulture And Food Research Institute Of New Zealand Limited | Use of massoialactone for inhibition of fungal growth |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114258924A (en) * | 2021-12-30 | 2022-04-01 | 新疆农业科学院微生物应用研究所(中国新疆—亚美尼亚生物工程研究开发中心) | Application of trichoderma hedgehog in preparation of medicine for preventing and treating pear black spot |
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