CN113180042A

CN113180042A - Application of paeonol as agricultural bactericide

Info

Publication number: CN113180042A
Application number: CN202110453925.1A
Authority: CN
Inventors: 宋萍萍; 王雨; 韦敏; 颜露; 钱怡云
Original assignee: Institute of Botany of CAS
Current assignee: Institute of Botany of CAS
Priority date: 2021-04-26
Filing date: 2021-04-26
Publication date: 2021-07-30

Abstract

The invention discloses application of paeonol compound as an agricultural bactericide. The compound is used as a bactericide for preventing and treating agricultural fungal diseases, and can effectively prevent and treat crop diseases such as fusarium wilt, gray mold, gibberellic disease, gummy stem blight and the like. The agricultural bactericide prepared by paeonol as an active ingredient has the characteristics of good prevention and treatment effect, broad spectrum, low toxicity, no residue, no pollution and no public nuisance, and is particularly beneficial to environmental protection.

Description

Application of paeonol as agricultural bactericide

Technical Field

The invention belongs to the technical field of agriculture, and particularly relates to application of a compound paeonol as an active ingredient in preparation of an agricultural bactericide.

Background

Diseases of crops are not easy to be detected by people in time like pests and weeds, so that neglect and difficulty in prevention and control are caused, and the damage is more serious. It is reported that plant diseases caused by pathogenic fungi alone account for as many as ten thousand in the world, and the loss caused accounts for 10% -30% of the annual total loss of crops. If plant diseases caused by viruses, bacteria, nematodes and the like are counted, the loss is more remarkable.

In order to meet the increasing demand of people for food and other living materials, chemicals have to be applied to protect crops from harmful organisms, of which bactericides play an important role. Natural products with agricultural bactericidal activity are generally easy to be absorbed and conducted by plants, and have little or no phytotoxicity to plants, so that the natural products are paid much attention.

The compound paeonol has CAS number of 552-41-0 and molecular formula of C₉H₁₀O₃166.18, molecular weight:

the paeonol compound has a melting point of 49-51 ℃, special smell and slightly spicy taste, is easily dissolved in ethanol and methanol, is dissolved in acetone, benzene, chloroform and carbon disulfide, is slightly dissolved in water, is dissolved in hot water, is insoluble in cold water, and can volatilize along with water vapor. The research on the activity of the compound for inhibiting the phytopathogen is less.

Disclosure of Invention

The invention aims to explore the application of paeonol as an active ingredient in preparing agricultural bactericides.

A large number of researches prove that the agricultural bactericide prepared by using the compound as an active ingredient has excellent bactericidal activity, can be applied to control fungal diseases on various crops, and has wide bacteriostatic spectrum, such as: blight, gray mold, head blight, gummy stem blight.

Paeonol can be obtained by separating and purifying natural plant, or by chemical synthesis. The compound is used as an active ingredient, a pesticide auxiliary agent and an excipient to be prepared into any formulation allowed on pesticides. It can also be compounded with other pesticides, and mixed with pesticide adjuvant and excipient to make any dosage form allowable on pesticides. The preparation formulation can be missible oil, a suspending agent, an aqueous emulsion, wettable powder, a microemulsion, water dispersible granules and the like. The used auxiliary agent can adopt calcium dodecyl benzene sulfonate, pyrrolidone, polyoxyethylene polyoxypropylene block compound, nonylphenol polyoxyethylene ether and the like, and the excipient can be cyclohexanone, solvent oil, water, propylene glycol, deionized water and the like. Other pesticides used for compounding can be organic bactericides, such as triazole bactericides triadimenol; botanical bactericides such as allicin, osthole, etc.

Compared with the prior art, the invention has the beneficial effects that: the paeonol as the bactericide has wide antibacterial spectrum and long lasting period, and is an ideal bactericide in agriculture. Compared with common chemical pesticides, the plant extract is derived from natural plants, has the characteristics of low toxicity, no residue, no pollution and no public nuisance for various economic crops such as grains, melons, fruits, vegetables and the like, and is more beneficial to environmental protection.

Detailed Description

The effects of the present invention are further illustrated by the following specific embodiments:

example 1: paeonol for preventing and treating agricultural diseases

Test strains Rhizoctonia solani (A)Thanatephorus cucumeris)Separating from rice sheath blight disease leaves; sclerotinia sclerotiorum (A) and (B)Sclerotioia selerotiorum) Separating from sclerotinia rot of colza strain; botrytis cinerea (A. cinerea)Botrytis cinerea) Separating from strawberry gray mold; fusarium graminearum (F.), (Fusarium graminearum) Separating from wheat scab; colletotrichum gloeosporioides (B)Colletotrichum gloeosporioides) Separating from grape anthracnose disease plants; fusarium moniliforme (F.), (Fusarium moniliforme) Is separated from the rice bakanae disease strain. The six strains are provided by the plant protection institute of Nanjing university of agriculture. Shell fungus of black rot melon (Didymella bryoniae) Magnaporthe grisea separated from gummy stem blight of watermelon (Magnaporthe oryzae) Is separated from rice blast disease plants, and the two strains are provided by plant research institute of China academy of sciences in Jiangsu province. Fusarium oxysporum (F.), (Fusarium oxysporum) Verticillium dahliae separated from cotton wilt disease plant (A), (B), (C)Verticillium dahliae) Is separated from cotton verticillium wilt disease plants and is provided by the plant protection of agricultural academy of Jiangsu province.

The test compound paeonol is dissolved in DMSO to prepare a mother solution of 1000 mu g/mL, and the mother solution is stored for later use. PDA culture medium, 200g peeled potato is cut into blocks, boiled for 10min, juice is taken, 20g agar is added, heating is carried out until melting, 20g glucose is added, and water is added for constant volume until 1000 mL.

Inhibition of hyphal growth by hyphal growth rate assayTaking a bacterium dish (diameter is 5mm) from the edge of a test bacterium pre-cultured on a PDA plate, uniformly mixing a test reagent mother liquor with a culture medium to ensure that the final concentrations of the test reagents are respectively 70, 60, 50, 40, 30, 20 and 0ppm, pouring the mixed liquor into sterilized culture dishes with the diameters of 9cm to prepare the plate, inoculating one bacterium dish in each dish, and repeating the treatment for 3 times, wherein the hypha faces upwards. And (4) culturing at 25 ℃ until the control colonies are more than two thirds of the full culture dish, and measuring the colony growing diameter by a cross method. The growth inhibition rate was calculated from the average colony diameter (mm). The obtained data is analyzed and processed by DPS V3.01 professional version software to obtain a standard curve and EC₅₀The value is obtained.

The results show that the compound paeonol can effectively prevent and treat fusarium wilt, gray mold, gibberellic disease and gummy stem blight, has good effect on the fusarium wilt and inhibits medium-concentration EC₅₀The concentration of the active component is 27.0894 mg/L, and the specific experimental result is shown in Table 1. Has weak inhibiting effect on other test bacteria, such as sclerotinia sclerotiorum, banded sclerotial blight, anthracnose, bakanae disease, verticillium wilt and rice blast.

TABLE 1 bacteriostatic rate of the compounds on agricultural diseases

Plant pathogenic bacteria	Regression equation	R	EC₅₀(mg/L)	95% confidence interval
					Fusarium oxysporum	y=0.2747+3.6814x	0.9793	27.0894	24.1761~30.3538
Botrytis cinerea	y=2.4663+4.1279x	0.9961	64.3753	58.6911~70.6099
					Fusarium graminearum	y=2.7890+1.0365x	0.9925	135.8639	98.9134~186.6178
Pediococcus cucurbitae	y=2.4391+1.1365x	0.9808	179.1505	114.0002~281.5339

Example 2:

weighing the compound and conventional auxiliary materials such as lignin, diatomite and the like, and preparing the wettable powder with the effective component concentration of 2% according to a conventional process.

Example 3:

the compound is weighed and mixed with conventional auxiliary materials such as calcium dodecyl benzene sulfonate, phenethyl phenol polyoxyethylene ether, solvent oil and the like according to a conventional process to prepare missible oil with the concentration of the effective component of 2 percent.

Example 4:

the compound and conventional auxiliary materials such as sodium lignosulphonate, kaolin and the like are weighed and prepared into water dispersible granules with the effective component concentration of 2% according to a conventional process.

Example 5:

weighing the compound, solvent oil, pyrrolidone, xanthan gum and other conventional auxiliary materials, and preparing the mixture into an aqueous emulsion with the concentration of an effective component of 1% according to a conventional process.

Example 6:

weighing the compound and conventional auxiliary materials such as calcium dodecyl benzene sulfonate, pyrrolidone and the like, and preparing the microemulsion with the concentration of the effective component of 1% according to the conventional process.

Example 7:

according to the weight ratio of 1: 5, weighing the compound and the triadimenol, and preparing the compound and the triadimenol with conventional auxiliary materials such as solvent oil, pyrrolidone, xanthan gum and the like into the aqueous emulsion with the effective component concentration of 5 percent according to a conventional process.

Example 8:

according to the weight ratio of 6: 1, weighing the compound and the allicin, and preparing the compound and the allicin with conventional auxiliary materials such as solvent oil, pyrrolidone, xanthan gum and the like into an aqueous emulsion with the concentration of an effective component of 5 percent according to a conventional process.

Example 9:

according to the weight ratio of 5: 1, weighing the compound and osthole, and mixing with solvent oil, pyrrolidone, xanthan gum and other conventional auxiliary materials according to a conventional process to prepare the aqueous emulsion with the effective component concentration of 5%.

Claims

1. Application of paeonol as active ingredient in preparing agricultural bactericide is provided.

2. The use according to claim 1, characterized in that the compound is used as an active ingredient and pesticide adjuvant and excipient to be prepared into any one of pesticide allowable dosage forms; or the compound is used as an active ingredient and is prepared into any one of pesticide allowable dosage forms together with other pesticides and pesticide auxiliary agents and excipients.

3. Use according to claim 2, characterized in that the formulation is an emulsifiable concentrate, a suspension, an aqueous emulsion, a wettable powder, a microemulsion or a water dispersible granule; the other pesticides are triazole fungicide triadimenol or botanical fungicides allicin and osthole.

4. The use according to claim 1, characterized in that the fungicide is used for controlling agricultural fungal diseases.

5. The use according to claim 4, characterized in that the agricultural fungal diseases are blight, gray mold, head blight, gummy stem blight.